Publications by authors named "Robinson Jeya Shakila"

14 Publications

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Dietary intake of trace elements from commercially important fish and shellfish of Thoothukudi along the southeast coast of India and implications for human health risk assessment.

Mar Pollut Bull 2021 Oct 9;173(Pt A):113020. Epub 2021 Oct 9.

Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Thoothukudi, India.

The concentrations of eight trace elements (chromium, cobalt, copper, zinc, arsenic, cadmium, mercury and lead) in14 commercially important fish and shellfish collected from Thoothukudi along the southeast coast of India was investigated using inductively coupled plasma mass spectrometry in order to assess the health risks associated with their consumption. The concentration of trace elements ranged from 0.001 to 39.5 μg/g. The estimated weekly intake of cadmium in seven fish and shellfish (0.0081-0.0996 mg/kg body weight) were above the provisional tolerable weekly intake set by the Joint FAO/WHO Expert Committee on Food Additives. The risk assessment analysis indicated that there was non- carcinogenic risk upon lifetime consumption of rock crab, C. natator (TTHQ >1) and carcinogenic risks upon lifetime consumption of S. jello, P. semisulcatus, P. sanguinolentus C. natator, Uroteuthis duvaceli, Sepia pharaonis and Cistopus indicus due to cadmium exposure indicating a potential health risk to the exposed consumers.
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http://dx.doi.org/10.1016/j.marpolbul.2021.113020DOI Listing
October 2021

Heavy metal concentrations in the macroalgae, seagrasses, mangroves, and crabs collected from the Tuticorin coast (Hare Island), Gulf of Mannar, South India.

Mar Pollut Bull 2021 Feb 24;163:111971. Epub 2021 Jan 24.

Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Tuticorin 628 008, Tamil Nadu, India.

This study investigates the concentration of heavy metals in the macroalgae, seagrasses, mangroves, and crabs collected from Hare Island, Gulf of Mannar Marine Biosphere Reserve. The concentration of heavy metals ranged between 0.06 (Hg)-259 (Fe) μg/g in macroalgae, 0.09 (Pb)-377 (Fe) μg/g in seagrasses, 0.112 (Cd)-122 (Fe) μg/g in mangroves, and 0.11 (Cd) -240 (Fe) μg/g in crabs. The levels of heavy metals in the analyzed samples were found below the maximum residual limits (MRLs) prescribed by various National and International agencies. The result suggests that exposure to the analyzed metals through macroalgae consumption does not cause potential health risks to consumers (target hazard quotient (THQ), estimated exposure dose (EED), and hazard index (HI) <1). Hence, this study concludes that macroalgae that grow in the Gulf of Mannar regions are safe for human consumption and are suitable to prepare food supplements and bioceutical products.
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http://dx.doi.org/10.1016/j.marpolbul.2021.111971DOI Listing
February 2021

Pesticides contamination in the Thamirabarani, a perennial river in peninsular India: The first report on ecotoxicological and human health risk assessment.

Chemosphere 2021 Mar 13;267:129251. Epub 2020 Dec 13.

Tamil Nadu Fisheries University, Nagapattinam, 611002, Tamil Nadu, India.

This study evaluates the distribution of pesticides and assesses the ecological and human health risks associated with pesticide residues concentration in the Thamirabarani River, the only perennial river in Tamil Nadu, India. Observed a variation in the pesticide concentration in the water (not detected (ND)-31.69 μg/L), sediments (ND-14.77 μg/kg), and fish (0.02-26.05 μg/kg). Endosulfan, aldrin, and endrin were the predominant organochlorine pesticides present in water, sediments, and fish. The average concentration of pesticides (except endosulfan) in water and sediments was found to be below the acceptable threshold as per the water and sediment quality guidelines, posing no ecological hazard to aquatic organisms. The calculated risk quotient and toxic unit (0.1 > TU/RQ ≤ 1) represent low-to-medium acute and chronic toxicity to the aquatic organisms inhabiting the river basin. The average concentration of pesticides in fish (Labeorohita) was also below the maximum residual limits set by the Codex Alimentarius Commission (CAC). However, the calculated daily intakes of endosulfan, aldrin, and endrin were above the CAC-acceptable daily intake guidelines. The human health risk assessment showed that children and adults exposed to pesticides in water and sediments through ingestion and dermal contact could have higher cancer risks (CR > 10) than inhalation. This study recommends implementing effective and routine pollution management schemes to avoid pesticide threats to aquatic and human health.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129251DOI Listing
March 2021

Heavy metal concentration in reef-associated surface sediments, Hare Island, Gulf of Mannar Marine Biosphere Reserve (southeast coast of India): The first report on pollution load and biological hazard assessment using geochemical normalization factors and hazard indices.

Mar Pollut Bull 2021 Jan 19;162:111838. Epub 2020 Nov 19.

Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Tuticorin 628 008, Tamil Nadu, India.

In this study, reef-associated surface sediment samples were collected from Hare Island in the Gulf of Mannar Marine Biosphere Reserve, Bay of Bengal and analyzed for heavy metal concentration. The sediment quality was evaluated based on the geochemical, biological, and ecological hazard indices. The mean concentration of heavy metals in the sediments ranged from 0.02 (Cd) to 26,262.87 mg/kg (Fe). Except for Cd and Hg, all other elements were found to be below the sediment quality guidelines and contamination level. The biological and ecological hazard (BEHI) revealed that most sediment samples (80%) fell under the low-risk category with 9% probability of toxicity to the marine flora and fauna. The overall contamination level of heavy metals in Hare Island suggested that the sediment could be grouped under low-risk category. Hence, this study recommends the need for a routine monitoring program in this region to maintain a clean and sustainable ecosystem in future.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111838DOI Listing
January 2021

Trace element concentrations in the organs of fish along the southeast coast of India.

Mar Pollut Bull 2021 Jan 9;162:111817. Epub 2020 Nov 9.

Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Thoothukudi, Tamil Nadu, India.

Trace element pollution in the marine system is a global concern as the exposure of marine organisms to this pollution results in bioaccumulation and further transfer of the trace elements to humans through food chain. In the present study, the distribution of trace elements, namely chromium, cobalt, nickel, iron, copper, zinc, arsenic, cadmium, mercury, and lead, in gills, bone, liver, and muscle of eight commercially important fish collected along the southeast coast of India was analyzed using an inductively coupled plasma mass spectrometer. The liver was the main organ of accumulation for copper, zinc, arsenic, cadmium, and mercury; bone for chromium, cobalt, and lead; gills for copper; and muscle for arsenic and mercury. The concentration of toxic trace elements such as arsenic, cadmium, mercury, and lead in the edible portion of fish was lower than the recommended International Legislation limits, indicating that the fish of this region are safe for consumption.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111817DOI Listing
January 2021

Risk assessment of organochlorine pesticides in seaweeds along the Gulf of Mannar, Southeast India.

Mar Pollut Bull 2020 Dec 7;161(Pt B):111709. Epub 2020 Oct 7.

Department of Fisheries Economics and Statistics, Faculty of Fishery Science, Kolkata, India.

Bioaccumulation of organochlorine pesticides (OCPs) in red, green, and brown seaweeds from the Gulf of Mannar was investigated using gas chromatography-mass spectrometry (GC-MS). The concentration of OCPs in all the seaweeds was in the order of endosulfan > endrin > HCH > DDT > aldrin > heptachlor > methoxychlor. The concentrations of HCH, endrin, endosulfan, and DDT in Sargassum wightii and Gelidiella acerosa, and HCH and endosulfan in Gracilaria verrucosa were above the limits prescribed by the European Commission. However, in Ulva lactuca, except for endosulfan, all other OCPs were below the permissible level. The bioaccumulation of OCPs in seaweeds was in the order of S. wightii > G. acerosa > Gracilaria verrucosa > U. lactuca. The results of comparative analysis of the levels of the investigated pesticides against the standard human health risk assessment revealed that pesticide exposure from seaweeds is hazardous to human health and the marine environment of the Gulf of Mannar.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111709DOI Listing
December 2020

Human health risk assessment of heavy metals in aquatic sediments and freshwater fish caught from Thamirabarani River, the Western Ghats of South Tamil Nadu.

Mar Pollut Bull 2020 Oct 29;159:111496. Epub 2020 Jul 29.

Tamil Nadu Fisheries University, Nagapattinam 611 002, Tamil Nadu, India.

Industrialization and advancements in agriculture are increasingly the cause of environmental concerns and need to be addressed. This study was carried out at four sampling sites, viz. Manimuthar (site 1), Tirunelveli (site 2), Srivaikuntam (site 3), and Authoor (site 4), along the Thamirabarani river. The heavy metal concentration in fish and sediments were ranged from 0.001 to 9.505 mg kg and 0.294 to 106.25 mg kg. The lifetime cancer risk (LCR) values for children and adults were found to be above the acceptable threshold value (ATV) and children were found to be more susceptible to health risks. For all sites except site 4, the LCR of toxic metals except Cd were within the ATV (10-10); the value of Cd was above the ATV, and poses a high cancer risk to the downstream inhabitants. This study advises routine heavy metal monitoring on aquatic organisms along these sites to implement regulatory standards.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111496DOI Listing
October 2020

Concentrations of trace elements in the organs of commercially exploited crustaceans and cephalopods caught in the waters of Thoothukudi, South India.

Mar Pollut Bull 2020 May 11;154:111045. Epub 2020 Mar 11.

Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Thoothukudi, Tamil Nadu, India.

Concentrations of trace elements (arsenic, cadmium, mercury, and lead) in tissues (muscle, gills, and digestive gland) of three commercially exploited crustaceans (Portunus sanguinolentus, Charybdis natator, and Penaeus semisulcatus) and three cephalopods (Doryteuthis sibogae, Sepia pharaonis, and Cistopus indicus) were examined. The animals were captured in the waters of Thoothukudi, and the tissues of six individuals of each species were analyzed using ICP-MS. The highest concentrations of arsenic (16.5 μg/g) and mercury (0.052 μg/g) were recorded in the digestive gland of C. natator, and cadmium (69.9 μg/g) and lead (0.351 μg/g) in the digestive gland of S. pharaonis. The edible portion of the crustaceans and cephalopods contained lower concentrations of trace elements, and these were below allowable limits set by the European Union. The edible parts of the investigated samples are safe for human consumption, but accidental contamination of the edible tissues with material from the digestive glands could lead to concerns relating to metal toxicity.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111045DOI Listing
May 2020

A single enzyme PCR-RFLP protocol targeting 16S rRNA/tRNA region to authenticate four commercially important shrimp species in India.

Food Chem 2018 Jan 23;239:369-376. Epub 2017 Jun 23.

Department of Fisheries Environment, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukkudi 628 008, India.

Food authenticity is an issue of major concern for food authorities, as mislabeling represents one of the major commercial frauds. In this study, a novel PCR-RFLP protocol was developed as a tool to authenticate four shrimp products of commercial importance belonging to the family, Penaeidae, viz. Litopenaeus vannamei, Penaeus monodon, P. semisulcatus and Fenneropenaeus indicus. PCR amplification was performed targeting 16S rRNA/tRNA region having an amplicon size of 530bp using the specific primers for shrimps, 16S-Cru4/16S-Cru3. Subsequent restriction analysis with a single restriction enzyme, Tsp5091, yielded distinct RFLP pattern for each species of shrimps having fragment sizes below 150bp. The unique RFLP patterns were also obtained in processed shrimp products without any degradation or alteration in the major fragments. The method was also validated with commercial shrimp products. Thus, the developed protocol can be performed within 8h using a single enzyme to authenticate four shrimp products of commercial significance.
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http://dx.doi.org/10.1016/j.foodchem.2017.06.132DOI Listing
January 2018

Antioxidant activities of squid protein hydrolysates prepared with papain using response surface methodology.

Food Sci Biotechnol 2016 30;25(3):665-672. Epub 2016 Jun 30.

2Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukkudi, 628008 India.

Squid protein hydrolysates (SPH) were prepared from the Indian squid using papain. Response surface methodology (RSM) was used for optimization of hydrolysis conditions, including temperature, time, and the enzyme-substrate ratio using DPPH radical scavenging activity as a response. The amino acid composition of SPH was compared with raw squid muscle. antioxidant activities were evaluated based on reducing power, metal chelation, ABTS, hydroxyl radical, and superoxide anion radical scavenging assays. SPH exhibited good ABTS radical scavenging activities of 96.50±0.90%, superoxide anion radical scavenging activities of 96.4±0.89%, reducing powers of 0.71±0.02, moderate hydroxyl radical scavenging activities of 64.03±2.11%, and metal chelating activities of 52.04±1.02%. antioxidant activities determined using a sardine minced model system showed 42% reduction in formation of secondary oxidative products as thiobarbituric acid reactive substances (TBARS), almost equivalent to reduction by ascorbic acid of 41.42% at 400 ppm.
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http://dx.doi.org/10.1007/s10068-016-0117-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6049156PMC
June 2016

Rheological, biocompatibility and osteogenesis assessment of fish collagen scaffold for bone tissue engineering.

Int J Biol Macromol 2016 Oct 19;91:51-9. Epub 2016 May 19.

Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukudi 628008, India. Electronic address:

In the present investigation, an attempt was made to find an alternative to mammalian collagen with better osteogenesis ability. Three types of collagen scaffolds - collagen, collagen-chitosan (CCH), and collagen-hydroxyapatite (CHA) - were prepared from the cartilage of Blue shark and investigated for their physico-functional and mechanical properties in relation to biocompatibility and osteogenesis. CCH scaffold was superior with pH 4.5-4.9 and viscosity 9.7-10.9cP. Notably, addition of chitosan and HA (hydroxyapatite) improved the stiffness (11-23MPa) and degradation rate but lowered the water binding capacity and porosity of the scaffold. Interestingly, CCH scaffolds remained for 3days before complete in-vitro biodegradation. The decreased amount of viable T-cells and higher level of FAS/APO-1 were substantiated the biocompatibility properties of prepared collagen scaffolds. Osteogenesis study revealed that the addition of CH and HA in both fish and mammalian collagen scaffolds could efficiently promote osteoblast cell formation. The ALP activity was significantly high in CHA scaffold-treated osteoblast cells, which suggests an enhanced bone-healing process. Therefore, the present study concludes that the composite scaffolds prepared from fish collagen with higher stiffness, lower biodegradation rate, better biocompatible, and osteogenesis properties were suitable biomaterial for a bone tissue engineering application as an alternative to mammalian collagen scaffolds.
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http://dx.doi.org/10.1016/j.ijbiomac.2016.05.067DOI Listing
October 2016

Effect of additives in the shelflife extension of chilled and frozen stored Indian octopus (Cistopus indicus).

J Food Sci Technol 2016 Feb 14;53(2):1348-54. Epub 2015 Nov 14.

Department of Fish Quality Assurance and Management, Fish Quality Monitoring and Certification Centre, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Tuticorin, 628 008 India.

In this study, the effect of commercial additives viz. cafodos and altesa employed to treat Indian octopus (Cistopus indicus) was examined during chilled and frozen storage. Shelf lives of treated and untreated octopus in ice were 6 and 8 days, respectively in ice. Treated and untreated frozen octopus had a shelf life of 40 days. Autolytic and microbiological changes were not controlled by the additives, as evidenced through rapid reduction in non-protein nitrogen (NPN) and α-amino nitrogen (α-AN) compounds; as well as accumulation of water soluble ammoniacal nitrogen and total volatile base- nitrogen (TVB-N) compounds. Loss of texture and colour were the major quality defects noticed in treated octopus as a result of enhanced protein solubility. Therefore, the additives approved for use in octopus neither enhanced the shelf life nor improved the sensory quality.
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http://dx.doi.org/10.1007/s13197-015-1930-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4837716PMC
February 2016

Skin, bone and muscle collagen extraction from the trash fish, leather jacket (Odonus niger) and their characterization.

J Food Sci Technol 2013 Dec 6;50(6):1106-13. Epub 2011 Aug 6.

Department of Fish Processing Technology, Fisheries College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tuticorin, 628 008 India.

Acid soluble (ASC) and pepsin soluble (PSC) collagens were extracted from the skin, bone and muscle of a trash fish, leather jacket (Odonus niger) by three different extraction methods. Method I gave 46-50% yield for ASC, Method II gave 49-58% yield for both ASC and PSC and Method III gave 64-71% yield for PSC. The addition of pepsin had increased the yield by 30-45%. The yields of collagen from skin and bone were higher than muscle. SDS-PAGE pattern revealed that skin and bone collagen as Type I collagen with a typical (α1)2α2 chains and muscle collagen as Type V collagen with a typical α1α3α2 chains. Td values of bone and muscle collagen were high (30-32 °C) compared to skin collagen (27-28 °C). The higher imino acids (190 residues/1,000 residues) were found responsible for the higher Td values. The trash fish, leather jacket can therefore be exploited effectively for collagen as it has got good thermal properties for pharmaceutical and biomedical applications.
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http://dx.doi.org/10.1007/s13197-011-0440-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3791228PMC
December 2013

Multiplex polymerase chain reaction-based assay for the specific detection of toxin-producing Vibrio cholerae in fish and fishery products.

Appl Microbiol Biotechnol 2011 May 1;90(3):1111-8. Epub 2011 Mar 1.

Department of Fish Processing Technology, Fisheries College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tuticorin 628 008, India.

A multiplex polymerase chain reaction (MPCR)-based assay was developed for the simultaneous detection of Vibrios using the genus-specific RNA polymerase subunit A (rpoA) gene and specific detection of toxin-producing Vibrio cholerae strains using two sets of primer based on cholera toxin subunit A (ctxA) and repeat in toxin subunit A (RtxA)-producing genes. The MPCR method developed is applicable to both the simultaneous and the two-step detection of genus Vibrio total and toxigenic V. cholerae species. This assay was specific as no amplification occurred with the other bacterial pathogens tested. The sensitivity of the assay was tested by artificially spiking the shrimp homogenate with the toxigenic strain of V. cholerae (NICED 16582) in different dilutions. The developed MPCR assay could detect three cells of V. cholerae in 12 h pre-enrichment in APW. The proposed method is rapid, sensitive, and specific for the detection of Vibrio genus as well as toxin-producing V. cholerae strains in environmental samples.
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http://dx.doi.org/10.1007/s00253-011-3175-9DOI Listing
May 2011
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