Publications by authors named "Richard Tagne Simo"

6 Publications

  • Page 1 of 1

A new flavonoid glycoside from (Loranthaceae) and evaluation of anticancer activity of extract and some isolated compounds.

Nat Prod Res 2021 Aug 12:1-9. Epub 2021 Aug 12.

UFR Sciences Fondamentales et Appliquées, Team Récepteurs, Régulations, Cellules Tumoraales (2RCT)-EA 3842 CAPTuR, Pôle Biologie Santé-Bât. B36/B37, University of Poitiers, Poitiers, France.

The present work describes the isolation and anticancer activity of which is a hemi parasitic plant harvested on , the host plant. Phytochemical study afforded a new flavonoid glycoside, tapinantoside () isolated for the first time from natural source, alongside six known compounds (-). Structure of compounds were elucidated by extensive spectroscopic analyses including 1 D and 2 D NMR, mass spectrometry and by comparison with literature data. The anticancer activity of extract and some isolated compounds were evaluated on glioblastoma (U87MG, C6) and prostate (PC-3) cancer cells. The methanol leaves extract showed good anticancer activity against U87 (IC = 21.40 µg/mL) and PC-3 cells (IC = 10.26 µg/mL). Compound powerfully inhibits the proliferation of C6 (IC = 38.84 µM) and PC-3 cells (IC = 21.33 µM), while its effect was moderated on U87MG cells. Compound and were not active on all tested cancer cell lines.
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http://dx.doi.org/10.1080/14786419.2021.1963243DOI Listing
August 2021

Awareness of Breast Cancer Screening among the Medical and General Population of the North Region of Cameroon.

Int J Breast Cancer 2021 27;2021:6663195. Epub 2021 Jul 27.

Department of Biochemistry University of Dschang, Cameroon.

Breast cancer has become a real public health problem in Cameroon, particularly in rural areas due to late diagnosis, resulting partly from the absence of national screening programs. This work is aimed at assessing breast cancer awareness in the North Region of Cameroon. Participants were selected in six health centers surrounding the rural area of Garoua, North Region, Cameroon, and administered a questionnaire aimed at assessing their awareness about breast cancer risk factors and screening. Out of the 475 women (including 37 medical personnel) interviewed, 45.5% attended at least secondary school; 91.3% were aware of the disease with the main sources of information from those around them (64.8%), media (46.5%), and health professionals in health facilities (42.7%). 23.3% had misconceptions and myth-based ideas on the origin of the disease. Ignorance was the main reason preventing the performance of breast self-examination, and the high cost prevents individuals from going for mammography. The highest awareness rate was observed in employed women with higher level of education. Our study highlights the need to raise awareness among the populations in North Region, Cameroon, about the risk factors and clinical signs of breast cancer and the importance of screening practice for early diagnosis of breast cancer.
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http://dx.doi.org/10.1155/2021/6663195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8331314PMC
July 2021

Anticancer Activity of Combretum fragrans F. Hoffm on Glioblastoma and Prostate Cancer Cell Lines.

Asian Pac J Cancer Prev 2021 Apr 1;22(4):1087-1093. Epub 2021 Apr 1.

UFR Sciences Fondamentales et Appliquées, Team "Récepteurs, Régulations, Cellules Tumorales" (2RCT)-EA 3842 CAPTuR, Pôle Biologie Santé-Bât. B36/B37, University of Poitiers, 1 rue Georges Bonnet-TSA, France.

Background: Cancer incidence has been growing in an alarming rate worldwide and new therapeutics are needed, particularly for intractable and chemoresistant cases. We evaluated the cytotoxic effects of Combretum fragrans F. Hoffm (Combretaceae) on glioblastoma (U87MG and C6) and prostate (PC-3) cancer cell lines.

Methods: The cytotoxic effect of the methanolic extract of the stem bark of Combretum fragrans was assessed using XTT (2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide) test. Expressions of Akt and ERK1/2 were determined using Western blot technique, while Caspase-3/7 kits were used to evaluate caspase-3/7 activity.

Results: C. fragrans extract inhibited the proliferation of U87 (IC50 = 20.13 µg/mL), C6 (IC50 = 12.17 µg/mL), and PC-3 (IC50 = 11.50 µg/mL) cells. Treatment with the extract resulted in lower levels (p < 0.001) of phospho-ERK1/2 and phospho-Akt in U87 cells, and instead, higher levels of phospho-ERK1/2 (p < 0.001) in C6 and PC-3 cells. An increase in caspase-3/7 activity was observed, mainly after 24 hours of treatment, indicating the activation of apoptotic processes.

Conclusion: Altogether, these results suggest that C. fragrans have potent anticancer properties. This plant should be further investigated for developing new anticancer drugs.
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http://dx.doi.org/10.31557/APJCP.2021.22.4.1087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325120PMC
April 2021

Emerging data supporting stromal cell therapeutic potential in cancer: reprogramming stromal cells of the tumor microenvironment for anti-cancer effects.

Cancer Biol Med 2020 11 15;17(4):828-841. Epub 2020 Dec 15.

Department of Medicine, University of Verona, Section of Hematology, Stem Cell Research Laboratory, Verona 37134, Italy.

After more than a decade of controversy on the role of stromal cells in the tumor microenvironment, the emerging data shed light on pro-tumorigenic and potential anti-cancer factors, as well as on the roots of the discrepancies. We discuss the pro-tumorigenic effects of stromal cells, considering the effects of tumor drivers like hypoxia and tumor stiffness on these cells, as well as stromal cell-mediated adiposity and immunosuppression in the tumor microenvironment, and cancer initiating cells' cellular senescence and adaptive metabolism. We summarize the emerging data supporting stromal cell therapeutic potential in cancer, discuss the possibility to reprogram stromal cells of the tumor microenvironment for anti-cancer effects, and explore some causes of discrepancies on the roles of stromal cells in cancer in the available literature.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0133DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721102PMC
November 2020

Mesenchymal stromal cells' role in tumor microenvironment: involvement of signaling pathways.

Cancer Biol Med 2017 May;14(2):129-141

Department of Biomedical Sciences, University of Ngaoundere, Ngaoundere 454, Cameroon.

Mesenchymal stromal cells (MSCs) are adult multipotent stem cells residing as pericytes in various tissues and organs where they can differentiate into specialized cells to replace dying cells and damaged tissues. These cells are commonly found at injury sites and in tumors that are known to behave like " wounds that do not heal." In this article, we discuss the mechanisms of MSCs in migrating, homing, and repairing injured tissues. We also review a number of reports showing that tumor microenvironment triggers plasticity mechanisms in MSCs to induce malignant neoplastic tissue formation, maintenance, and chemoresistance, as well as tumor growth. The antitumor properties and therapeutic potential of MSCs are also discussed.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2016.0033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5444925PMC
May 2017

Developmental pathways associated with cancer metastasis: Notch, Wnt, and Hedgehog.

Cancer Biol Med 2017 May;14(2):109-120

Department of Biomedical Sciences, University of Ngaoundere, Ngaoundere 454, Cameroon.

Master developmental pathways, such as Notch, Wnt, and Hedgehog, are signaling systems that control proliferation, cell death, motility, migration, and stemness. These systems are not only commonly activated in many solid tumors, where they drive or contribute to cancer initiation, but also in primary and metastatic tumor development. The reactivation of developmental pathways in cancer stroma favors the development of cancer stem cells and allows their maintenance, indicating these signaling pathways as particularly attractive targets for efficient anticancer therapies, especially in advanced primary tumors and metastatic cancers. Metastasis is the worst feature of cancer development. This feature results from a cascade of events emerging from the hijacking of epithelial-mesenchymal transition, angiogenesis, migration, and invasion by transforming cells and is associated with poor survival, drug resistance, and tumor relapse. In the present review, we summarize and discuss experimental data suggesting pivotal roles for developmental pathways in cancer development and metastasis, considering the therapeutic potential. Emerging targeted antimetastatic therapies based on Notch, Wnt, and Hedgehog pathways are also discussed.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2016.0032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5444923PMC
May 2017
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