Publications by authors named "Ricardo de Souza Magini"

18 Publications

  • Page 1 of 1

Effect of dexamethasone as osteogenic supplementation in in vitro osteogenic differentiation of stem cells from human exfoliated deciduous teeth.

J Mater Sci Mater Med 2021 Jan 19;32(1). Epub 2021 Jan 19.

Center for Research on Dental Implants, Federal University of Santa Catarina, Delfino Conti Street, Florianópolis, 88040-900, Brazil.

In in vitro culture systems, dexamethasone (DEX) has been applied with ascorbic acid (ASC) and β-glycerophosphate (βGLY) as culture media supplementation to induce osteogenic differentiation of mesenchymal stem cells. However, there are some inconsistencies regarding the role of DEX as osteogenic media supplementation. Therefore, this study verified the influence of DEX culture media supplementation on the osteogenic differentiation, especially the capacity to mineralize the extracellular matrix of stem cells from human exfoliated deciduous teeth (SHED). Five groups were established: G1-SHED + Dulbecco's Modified Eagles' Medium (DMEM) + fetal bovine serum (FBS); G2-SHED + DMEM + FBS + DEX; G3-SHED + DMEM + FBS + ASC + βGLY; G4-SHED + DMEM + FBS + ASC + βGLY + DEX; G5-MC3T3-E1 + α Minimal Essential Medium (MEM) + FBS + ASC + βGLY. DNA content, alkaline phosphatase (ALP) activity, free calcium quantification in the extracellular medium, and extracellular matrix mineralization quantification through staining with von Kossa, alizarin red, and tetracycline were performed on days 7 and 21. Osteogenic media supplemented with ASC and β-GLY demonstrated similar effects on SHED in the presence or absence of DEX for DNA content (day 21) and capacity to mineralize the extracellular matrix according to alizarin red and tetracycline quantifications (day 21). In addition, the presence of DEX in the osteogenic medium promoted less ALP activity (day 7) and extracellular matrix mineralization according to the von Kossa assay (day 21), and more free calcium quantification at extracellular medium (day 21). In summary, the presence of DEX in the osteogenic media supplementation did not interfere with SHED commitment into mineral matrix depositor cells. We suggest that DEX may be omitted from culture media supplementation for SHED osteogenic differentiation in vitro studies.
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http://dx.doi.org/10.1007/s10856-020-06475-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815568PMC
January 2021

PLGA+HA/βTCP Scaffold Incorporating Simvastatin: A Promising Biomaterial for Bone Tissue Engineering.

J Oral Implantol 2021 Apr;47(2):93-101

Department of Dentistry, Federal University of Santa Catarina, Santa Catarina, Brazil.

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite/β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m/m) of this medication. Scaffolds were synthesized in a cylindric shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in phosphate-buffered saline at 37°C under constant stirring for 7, 14, 21, and 28 days. Nondegraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro-, meso-, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.
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http://dx.doi.org/10.1563/aaid-joi-D-19-00148DOI Listing
April 2021

Implantoplasty Enhancing Peri-implant Bone Stability Over a 3-Year Follow-up: A Case Series.

Int J Periodontics Restorative Dent 2020 Jan/Feb;40(1):e1-e8

Implantoplasty has been claimed as a promising strategy to treat peri-implantitis and prevent progressive peri-implant bone loss. Consequently, the aim of the present case series is to exhibit the clinical outcomes of a 3-year-follow-up resective and implantoplasty therapy applying a novel platform-switch concept to preserve peri-implant tissue integrity and counteract progressive bone loss. Four patients who underwent dental implant therapy and were diagnosed with peri-implantitis were treated through access flap surgery, a modified implantoplasty, bone recontouring, and surface decontamination. The radiographic and clinical parameters recorded before and during the 3-year follow-up were: marginal bone loss (MBL) as the primary endpoint, bleeding on probing index (BOP), probing depth (PD), presence of suppuration, pain, mobility, and fracture. The 3-year follow-up exhibited peri-implant bone stability in all cases (100%) showing radiographically an MBL reduction (mean) of 0.8 ± 0.5 mm (mesial) and 0.5 ± 0.3 mm (distal). Mean PD reduction was 4.75 ± 1 mm and mean BOP was reduced by 71%. Pain and suppuration were resolved in all cases. None of the cases reported implant fracture or mobility after the modified implantoplasty therapy. The present case series demonstrated that this modified implantoplasty can be more than a surface decontamination therapy where the narrow and smooth exposed implant surface can counteract peri-implantitis alterations providing favorable biologic conditions to maintain stability of peri-implant tissues.
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http://dx.doi.org/10.11607/prd.3849DOI Listing
December 2019

Retinoic acid increases the effect of bone morphogenetic protein type 2 on osteogenic differentiation of human adipose-derived stem cells.

J Appl Oral Sci 2019 Feb 21;27:e20180317. Epub 2019 Feb 21.

Universidade Federal de Santa Catarina, Programa de Pós-Graduação em Farmácia, Departamento de Ciências Farmacêuticas, Florianópolis, Santa Catarina,Brasil.

Background: Bone morphogenetic protein type 2 (BMP-2) and retinoic acid (RA) are osteoinductive factors that stimulate endogenous mechanisms of bone repair which can be applied on management of osseous defects in oral and maxillofacial fields.

Objective: Considering the different results of RA on osteogenesis and its possible use to substitute/potency BMP-2 effects, this study evaluated the outcomes of BMP-2, RA, and BMP-2+RA treatments on in vitro osteogenic differentiation of human adipose-derived stem cells (ASCs) and the signaling pathway(s) involved.

Material And Methods: ASCs were treated every other day with basic osteogenic medium (OM) alone or supplemented with BMP-2, RA, or BMP-2+RA. Alkaline phosphatase (ALP) activity was determined using the r-nitrophenol method. Extracellular matrix mineralization was evaluated using von Kossa staining and calcium quantification. Expression of osteonectin and osteocalcin mRNA were determined using qPCR. Smad1, Smad4, phosphorylated Smad1/5/8, BMP-4, and BMP-7 proteins expressions were analyzed using western blotting. Signaling pathway was evaluated using the IPA® software.

Results: RA promoted the highest ALP activity at days 7, 14, 21, and 28, in comparison to BMP-2 and BMP-2+RA. BMP-2+RA best stimulated phosphorylated Smad1/5/8 protein expression at day 7 and Smad4 expression at days 7, 14, 21, and 28. Osteocalcin and osteonectin mRNA expressions were best stimulated by BMP-2+RA at day 7. Matrix mineralization was most improved by BMP-2+RA at days 12 and 32. Additionally, BMP-2+RA promoted the highest BMP signaling pathway activation at days 7 and 14, and demonstrated more activation of differentiation of bone-forming cells than OM alone.

Conclusions: In summary, RA increased the effect of BMP-2 on osteogenic differentiation of human ASCs.
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http://dx.doi.org/10.1590/1678-7757-2018-0317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382324PMC
February 2019

Versatility and Importance of Bichat's Fat Pad in Dentistry: Case Reports of Its Use in Occlusal Trauma.

J Contemp Dent Pract 2018 Jul 1;19(7):888-894. Epub 2018 Jul 1.

Postgraduate Program in Dentistry, Center for Research and Education on Dental Implants, School of Dentistry, Federal University of Santa Catarina, UFSC Florianopolis, Santa Catarina, Brazil.

Introduction: The knowledge of the anatomy surrounding Bichat's fat pad, as well as its clinical applications, is essential to indicate and to safely perform its removal. This surgery is indicated not only for esthetic purposes, but also for functional reasons. When used properly, Bichat's fat pad is composed of stem cells that have a similar phenotype to adipose stem cells, useful in the treatment of pathologies and/or complications, such as maxillary sinus membrane perforation, oroantral/oronasal communications, peri-implantitis, ulcers, fibrosis of the oral mucosa, soft tissue reconstruction, among others. Due to its location, it is prone to suffer clinically significant pathologies, as well as constant trauma.

Aim: The aim of this study is to report two clinical cases and subsequent follow-ups, where bichectomy was performed to avoid dental trauma to mucosal tissues during the masticatory function. Also, literature review on the application of Bichat's fat pad in dentistry is provided.

Case Report: Two female patients (20 and 24 years) reported discomfort and constant pain in the oral mucosa caused by dental trauma. At the clinical examination, patient presented augmented and injured mucosa. The surgical sequence of Bichat's fat pad removal, as well as the extra-/intraoral photographic follow-up (8, 15, 30, and 180 days) of the patients is described.

Conclusion: In order to indicate and/or accomplish surgical procedures involving Bichat's fat pad, it is fundamental to know its anatomy and possible applications, not only for esthetic purposes, but also for functional purposes. The patients showed evident improvements following the removal of Bichat's fat pad.

Clinical Significance: Application of Bichat's fat and its removal should be evaluated, being an alternative in patients who constantly undergo mucosal injury during masticatory function.
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July 2018

Impact of laboratory treatment with coloring and fluorescent liquids on the optical properties of zirconia before and after accelerated aging.

J Prosthet Dent 2018 Aug 15;120(2):276-281. Epub 2018 Mar 15.

Professor, Department of Dentistry, Federal University of Santa Catarina, Florianópolis, Brazil.

Statement Of Problem: Laboratory procedures, such as dipping in coloring and fluorescent liquids, can be used to improve the optical properties of zirconia. However, information is lacking on the effect of these liquids.

Purpose: The purpose of this in vitro study was to evaluate the color differences and degree of fluorescence of zirconia (3Y-TZP) treated with coloring and fluorescent liquids before and after an accelerated aging protocol.

Material And Methods: Forty disk-shaped specimens of 3Y-TZP were fabricated by milling and separated according to the laboratory treatment performed: white zirconia (control group); zirconia treated with coloring liquid (A2 group); zirconia treated with fluorescent liquid (fluorescent group); and zirconia treated with both liquids (A2 fluorescent group). The L*a*b* coordinates before aging (T) were obtained with a spectrophotometer, and the degree of fluorescence was measured. The disks were subjected to accelerated aging for 1 hour (T) and 5 hours (T). Measurements were made before and after each time interval. Color differences (ΔE) were calculated using the CIEDE2000 formula and analyzed by 2-way ANOVA. Lightness (ΔL'), chroma (ΔC'), and hue differences (ΔH') were analyzed by multivariate ANOVA. Degrees of fluorescence were obtained as percentages and were analyzed by 2-way ANOVA. Multiple comparisons were performed by the Tukey HSD test (α=.05).

Results: Color differences were observed when 3Y-TZP disks were treated with coloring (7.91 ΔE), with fluorescent liquid (5.81 ΔE), and with both liquids (5.52 ΔE). Accelerated aging resulted in color differences in the T A2 group (6.74 ΔE) and at both times evaluated in the fluorescent group (T=8.59 ΔE and T=8.47 ΔE) (P<.001). In the A2 fluorescent group, the degree of fluorescence was not influenced significantly (P>.05). The use of fluorescent liquid influenced the degree of fluorescence in the fluorescent group (T=20%).

Conclusions: Significant differences in color, lightness, chroma, and hue were achieved in all tested groups before and after aging. The degree of fluorescence was statistically different only in the fluorescent group and was not influenced by accelerated aging.
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http://dx.doi.org/10.1016/j.prosdent.2017.10.017DOI Listing
August 2018

Effect of γ-lactones and γ-lactams compounds on Streptococcus mutans biofilms.

J Appl Oral Sci 2018 22;26:e20170065. Epub 2018 Feb 22.

Centro de Ensino e Pesquisa em Implantes Dentários, Departamento de Odontologia, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brasil.

Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses.

Objective: To assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation.

Material And Methods: We synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine. Compounds concentrations ranging from 0.17 up to 87.5 μg mL-1 were tested against S. mutans. We diluted the exponential cultures in TSB and incubated them (37°C) in the presence of different γ-lactones or γ-lactams dilutions. Afterwards, we measured the planktonic growth by optical density at 630 nm and therefore assessed the biofilm density by the crystal violet staining method.

Results: Twelve compounds were active against biofilm formation, showing no effect on bacterial viability. Only one compound was inactive against both planktonic and biofilm growth. The highest biofilm inhibition (inhibition rate above 60%) was obtained for two compounds while three other compounds revealed an inhibition rate above 40%.

Conclusions: Twelve of the thirteen compounds revealed effective inhibition of S. mutans biofilm formation, with eight of them showing a specific antibiofilm effect.
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http://dx.doi.org/10.1590/1678-7757-2017-0065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831975PMC
April 2018

Lip Repositioning Technique With Smile Elevator Muscle Containment - A Novel Cosmetic Approach for Gummy Smile: Case Report.

Compend Contin Educ Dent 2017 Nov/Dec;38(10):e9-e12

Associate Professor, School of Dentistry, São Leopoldo Mandic, Campinas, Brazil.

Excessive gingival display (EGD) is a challenge for dentists attempting to provide their patients a pleasant smile. EGD associated with hyperactivity of the smile elevator muscles can be treated with various surgical techniques; regardless of which technique is used, to achieve a predictable result with long-term stability limiting upper lip movement when the patient smiles, a firm muscle containment is imperative. This report describes an innovative suture procedure associated with a lip repositioning technique aimed at maintaining the traction and containment of the smile elevator muscles. This case demonstrates a successful and stable result for excessive gingival exposure, addressing and satisfying a patient's esthetic concerns.
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October 2018

Ridge Preservation After Maxillary Third Molar Extraction Using 30% Porosity PLGA/HA/β-TCP Scaffolds With and Without Simvastatin: A Pilot Randomized Controlled Clinical Trial.

Implant Dent 2017 Dec;26(6):832-840

Full Professor, Post-graduate Program in Dentistry (PPGO), Center for Research and Education on Dental Implants (CEPID), School of Dentistry (ODT), Federal University of Santa Catarina, UFSC, Florianópolis, Santa Catarina, Brazil.

Objective: To evaluate clinically and radiographically, in humans, the healing of maxillary third molars postextraction sockets after application of different ridge preservation techniques 3 months after tooth extraction.

Materials And Methods: Twenty-six sockets (13 patients) were randomly assigned to 4 treatment modalities: deproteinized bovine bone mineral with 10% collagen (DBBM-C), poly(D,L-lactide-co-glycolide) with hydroxyapatite/β-TCP scaffold (PLGA/HA), PLGA/HA/β-TCP with 2.0% simvastatin scaffold (PLGA/HA/S), and spontaneous healing (control). Clinical complications were assessed, and cone-beam computed tomographies were taken in 5 patients 3 months after surgeries. For statistical purposes, the Fisher exact test was used (P < 0.05).

Results: After 3 months, 6 of 9 grafts from the PLGA/HA group were lost (P < 0.05). PLGA/HA/S' loss was only 2 of 8 (P > 0.05), but no loss was observed in the DBBM-C group. Pain was present in 3 of 8 sites that lost the graft (37.5%) (P > 0.05) and infection in 1 of 8 (12.5%) (P > 0.05), with these only occurring in the PLGA/HA group.

Conclusions: Poly (D, L-lactide-co-glycolide) with hydroxyapatite/β-TCP (PLGA/HA/β-TCP) scaffolds, with and without simvastatin, failed to obtain the initial expected results and presented more complications. Scaffolds with simvastatin showed to be superior, with less clinical complications than scaffolds without simvastatin.
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http://dx.doi.org/10.1097/ID.0000000000000655DOI Listing
December 2017

Biofilm Analysis of Retrieved Dental Implants after Different Peri-Implantitis Treatments.

Case Rep Dent 2017 9;2017:8562050. Epub 2017 Apr 9.

School of Dentistry, Center for Research on Dental Implants, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

The aim of the current study was to analyse the planktonic growth of on the surfaces of three implants retrieved after three different peri-implantitis treatments. Three implants from a male patient with high levels of bone loss were treated by mechanical debridement, chemical decontamination, and implantoplasty. After 4 months of follow-up, the implants were removed. The growth and biofilm formation were measured by spectrophotometry (OD) and scanning electron microscopy (SEM), after 48 hours of incubation. Results showed an average of planktonic growth over the implants of 0.21 nm (mechanical debridement), 0.16 nm (chemical decontamination), and 0.15 nm (implantoplasty). Data were analysed by ANOVA and Tukey's test ( < 0.05 for chemical decontamination and implantoplasty). Implantoplasty and chemical decontamination showed the lowest levels of planktonic growth, indicating a possible influence of the modification procedures on the titanium surface on the initial biofilm attachment.
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http://dx.doi.org/10.1155/2017/8562050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5401748PMC
April 2017

Modified Double-Papillae Flap Technique With Subepithelial Connective Tissue Graft for Root Coverage in the Esthetic Zone: Case Report.

Compend Contin Educ Dent 2017 Mar;38(3):e4-e8

Associate Professor, School of Dentistry-Federal University of Santa Catarina, Florianópolis, SC, Brazil.

Gingival recession can compromise the esthetic appearance, leading to functional problems, hypersensitivity, and root caries. Several techniques have been implicated for root coverage, which includes pedicle grafts, free gingival grafts, connective tissue grafts, and guided-tissue regeneration. The double-papillae flap associated with subepithelial connective tissue is a predictable technique to cover isolated areas with insufficient attached gingiva apical to a recession. This case report demonstrates a surgical alternative to the technique using a sling periosteal suture to stabilize the connective tissue and pedicle flap during the initial phase of healing, increasing the potential of this periodontal procedure for gingival recession coverage.
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March 2017

A Step-by-Step Management of Extraction Sites in Areas of Maxillary Sinus Pneumatization: A Literature Review and a Case Presentation of a New Surgical Technique.

J Int Acad Periodontol 2016 Oct 7;18:102-108. Epub 2016 Oct 7.

School of Dentistry, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.

Introduction: The rehabilitation of the posterior maxilla with implant-supported prosthesis is often complicated by pneumatization of the maxillary sinus. Bone grafting is commonly required in these cases. Over the years, a number of techniques have been developed for this type of reconstruction.

Aim: Present and discuss the possibility of alveolar bone regeneration for subsequent placement of oral implants using Fugazzotto's technique in combination with particulate autograft harvested from the mandibular ramus and a connective tissue pedicle flap to cover the graft.

Methods: A case of a 37-year-old woman with a molar perforated during endodontic treatment and indicated for extraction and implant placement is reported.

Result And Conclusion: The clinical case showed the possibility of grafting of extraction sites combined with atraumatic elevation of the maxillary sinus floor can be achieved using non-conventional techniques such as Fugazzotto's technique associated with alveolar bone regeneration.
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October 2016

Does the number of implants have any relation with peri-implant disease?

J Appl Oral Sci 2014 Sep-Oct;22(5):403-8

Department of Periodontics, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

Objective: The aim of this study was to evaluate the relationship between the number of pillar implants of implant-supported fixed prostheses and the prevalence of periimplant disease.

Material And Methods: Clinical and radiographic data were obtained for the evaluation. The sample consisted of 32 patients with implant-supported fixed prostheses in function for at least one year. A total of 161 implants were evaluated. Two groups were formed according to the number of implants: G1) ≤5 implants and G2) >5 implants. Data collection included modified plaque index (MPi), bleeding on probing (BOP), probing depth (PD), width of keratinized mucosa (KM) and radiographic bone loss (BL). Clinical and radiographic data were grouped for each implant in order to conduct the diagnosis of mucositis or peri-implantitis.

Results: Clinical parameters were compared between groups using Student's t test for numeric variables (KM, PD and BL) and Mann-Whitney test for categorical variables (MPi and BOP). KM and BL showed statistically significant differences between both groups (p<0.001). Implants from G1 - 19 (20.43%)--compared with G2 - 26 (38.24%)--showed statistically significant differences regarding the prevalence of peri-implantitis (p=0.0210).

Conclusion: It seems that more than 5 implants in total fixed rehabilitations increase bone loss and consequently the prevalence of implants with periimplantitis. Notwithstanding, the number of implants does not have any influence on the prevalence of mucositis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245752PMC
http://dx.doi.org/10.1590/1678-775720140055DOI Listing
August 2015

Creeping attachment involving dental implants: two case reports with a two-year follow-up from an ongoing clinical study.

Case Rep Dent 2014 3;2014:756908. Epub 2014 Sep 3.

Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.

Introduction. This paper describes case reports where coronal growth of soft tissue on implant threads was observed after surgery for soft tissue graft. This phenomenon is known as "creeping attachment." Methods. Two patients were submitted to gingival graft procedure including subepithelial connective tissue graft and masticatory mucosal graft. A two-year follow-up appointment was performed. Results. After a two-year follow-up gingival growth over titanium surfaces characterizing the "creeping attachment" phenomenon was observed. This gingival growth happened over abutment and threads surfaces. Conclusion. The creeping attachment phenomenon is possible over titanium surfaces and has not yet been reported in the relevant literature over this kind of structure.
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http://dx.doi.org/10.1155/2014/756908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4168148PMC
October 2014

The use of zygomatic implants for the rehabilitation of atrophic maxillas with 2 different techniques: Stella and Extrasinus.

Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011 Dec 8;112(6):e49-53. Epub 2011 Sep 8.

PhD program in Implantology, University Federal of Santa Catarina, Santa Catarina, Brazil.

The zygomatic implant anchorage is a surgical technique that provides a new perspective for patients with severe maxillary atrophy, increasing predictability and reduced cost of treatment, besides being a tool for the hardships of the rehabilitation of such a challenging region. This article describes 2 clinical cases with zygomatic implants with different techniques (Stella and Extrasinus) and both with immediate loading and accompanying clinical radiographic follow-up procedures of 12 and 24 months, respectively.
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http://dx.doi.org/10.1016/j.tripleo.2011.05.008DOI Listing
December 2011

Viability analysis of subepithelial connective tissue grafts subjected to a mechanical expansion process: a histologic study in dogs.

Int J Periodontics Restorative Dent 2011 Sep-Oct;31(5):e37-44

Department of Periodontics, Dentistry College of Federal University of Santa Catarina, Florianópolis, SC, Brazil.

The aim of this study was to histologically evaluate the viability of mechanically expanded subepithelial connective tissue grafts. Sixteen samples of palatal connective tissue were collected from eight beagle dogs. Half of the samples were subjected to the expansion procedure and used as subepithelial grafts in the canine region, and the samples not subjected to expansion were grafted at the contralateral side. After 60 days, biopsies were collected and examined histologically by light and confocal laser microscopy and immunohistochemically with anti-CD31 antibody for endothelial cells. There were no significant differences between the control and test groups. It was concluded that this new method to expand the area of connective tissue grafts was not only viable biologically, but also decreased surgical risks without increasing processing time.
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February 2012

Evaluation of the stress distribution in CFR-PEEK dental implants by the three-dimensional finite element method.

J Mater Sci Mater Med 2010 Jul 13;21(7):2079-85. Epub 2010 May 13.

Department of Pos-Graduation Course of Implantology, Universidade Federal do Paraná, Curitiba, Brazil.

CFR-PEEK (carbon fiber reforced-poly ether ether ketone) has been demonstrated to be excellent substitute titanium in orthopedic applications and can be manufactured with many physical, mechanical, and surface properties, in several shapes. The aim of this study was to compare, using the three-dimensional finite element method, the stress distribution in the peri-implant support bone of distinct models composed of PEEK components and implants reinforced with 30% carbon fiber (30% CFR-PEEK) or titanium. In simulations with a perfect bonding between the bone and the implant, the 30% CFR-PEEK presented higher stress concentration in the implant neck and the adjacent bone, due to the decreased stiffness and higher deformation in relation to the titanium. However, 30% CFR-PEEK implants and components did not exhibit any advantages in relation to the stress distribution compared to the titanium implants and components.
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http://dx.doi.org/10.1007/s10856-010-4084-7DOI Listing
July 2010

Platelet-rich plasma influence on human osteoblasts growth.

Clin Oral Implants Res 2005 Aug;16(4):456-60

Department of Dental Implantology, University of Santa Catarina, Florianópolis, Brazil.

Objective: The influence of progressively high concentrations of platelet-rich plasma (PRP) on human osteoblast hFOB1.19 proliferation was evaluated.

Material And Methods: The PRP was obtained from a human source. Two experiments were conducted. In the first one, PRP was diluted to 50%, 25%, 12.5% and 6.125% (v/v) with culture medium (Modified Eagle's Medium (MEM) : Ham's F12 Medium (HAM-F12%) and 1% antibiotics-antimicotic) supplemented with 10% of fetal bovine serum (FBS). In the second experiment, all conditions were identical except for the absence of FBS in the culture medium.

Results: The results of the osteoblast proliferation test were higher when stimulated by the 50% PRP dilution, with or without FBS. A further study is suggested to determine if concentrations above 50% could cause higher rates of osteoblast proliferation. In this study, the results were not statistically different (P<0.05) with 12.5% and 6.125% PRP dilutions. Additionally, it was shown that FBS is not necessary for PRP-mediated induction of osteoblast proliferation.

Conclusion: This study concluded that PRP promotes osteoblast proliferation and suggested its clinical application to bone graft procedures in implant dentistry.
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http://dx.doi.org/10.1111/j.1600-0501.2005.01145.xDOI Listing
August 2005