Publications by authors named "Reza Ghanbari"

39 Publications

Seroprevalence of Immunoglobulin M and G Antibodies against SARS-CoV-2 Virus: A Systematic Review and Meta-Analysis Study.

Iran J Immunol 2021 Mar;18(1):34-46

Student Research Committee, Faculty of Medicine, Shahid Beheshti University of MedicalSciences, Tehran, Iran.

Background: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a new global health threat.

Objectives: to analyze the effectiveness of the measurement of specific antibodies to SARS-CoV2 (IgM and IgG) for the diagnosis of COVID-19 and to analyze the rate of SARS-CoV2 seroprevalence in the population.

Methods: 11 relevant studies, published before June 5, 2020, were included in this meta-analysis. These studies were identified by searching the MEDLINE and Scopus databases. The final selected studies were analyzed using STATA version 14. Publication bias was examined using both Egger's test and Funnel plots. Moreover, the I² statistic has been used to evaluate and verify heterogeneity.

Results: The 11 relevant studies selected for the present meta-analysis cover a total of 996 infection cases. According to the results, the average rate of positive cases for IgM (AU/mL) was 2.10 (95% CI: 1.65-2.55; I2=92.2%), and the sensitivity in individuals with positive IgM test was 63% (95% CI: 47-79; I2=94.9%). In addition, the average rate of positive cases for IgG (AU/mL) was 67.44 (95% CI: 28.79-106.09; I2=99.4%), and the sensitivity in individuals with positive IgG test was 79% (95% CI: 67-90; I2=89.5%).

Conclusions: According to this analysis, detection of anti-SARS-CoV-2 IgM and IgG antibodies may assist early detection of SARS-CoV2 infection. Whether antibodies against SARS-CoV-2 confer protective immunity warrants further studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.22034/iji.2021.87723.1824DOI Listing
March 2021

Identification of dimensions and indicators of spiritual health: A qualitative study.

J Educ Health Promot 2020 29;9:352. Epub 2020 Dec 29.

Department of Laboratory Sciences, Faculty of Paramedical Sciences, Aja University of Medical Sciences, Tehran, Iran.

Introduction: Spiritual health is considered one of the important dimensions of health and has been given serious attention by the scientific community, World Health Organization, health discourse, and medical education. However, despite these considerations, there is a controversy in defining the nature of this category and its dimensions and indicators. The purpose of this study is to identify the indicators and dimensions of spiritual health for medical students.

Materials And Methods: This qualitative study with exploratory approach utilized conventional content analysis and individual in-depth interviews with experts in the area of spiritual health and spirituality in medical sciences selected through purposeful sampling. The study was conducted at Aja University of Medical Sciences in Tehran in 2018. The semi-structured interview was a tool used to achieve data saturation, that interviewed with 19 experts. Member check, credibility, and reliability were measured to increase the validity and reliability of the results. Inductive content analysis was used for the analysis of data in three main phases: preparation, organization, and categories and creating categories.

Results: Based on the findings of the interviews, 52 indicators were identified.Then, the indicators of spiritual health were identified in the following four categories) relationship with God, relationship with himself, relationship with others, relation with the environment (and these categories were divided inthree dimentions (cognitive, attitudes and emotions, behavioral).

Conclusion: The findings of the present study showed that in order to have spiritual health, it is important to pay attention to one's relationship with God and one's relationship with oneself, one's relationship with others, and one's relationship with the environment, and it is necessary to pay attention to it in medical science curricula.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/jehp.jehp_448_20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7871966PMC
December 2020

Metabolomics reveals biomarkers of opioid use disorder.

Transl Psychiatry 2021 Feb 4;11(1):103. Epub 2021 Feb 4.

Department of Nutrition, Nutrition Research Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Opioid use disorder (OUD) is diagnosed using the qualitative criteria defined by the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5). Diagnostic biomarkers for OUD do not currently exist. Our study focused on developing objective biological markers to differentiate chronic opiate users with OUD from chronic opiate users without OUD. Using biospecimens from the Golestan Cohort Study, we compared the metabolomics profiles of high opium users who were diagnosed as OUD positive with high opium users who were diagnosed as OUD negative. High opium use was defined as maximum weekly opium usage greater than or equal to the median usage (2.4 g per week), and OUD was defined as having 2 or more DSM-5 criteria in any 12-month period. Among the 218 high opium users in this study, 80 were diagnosed as OUD negative, while 138 were diagnosed as OUD positive. Seven hundred and twelve peaks differentiated high opium users diagnosed as OUD positive from high opium users diagnosed as OUD negative. Stepwise logistic regression modeling of subject characteristics data together with the 712 differentiating peaks revealed a signature that is 95% predictive of an OUD positive diagnosis, a significant (p < 0.0001) improvement over a 63% accurate prediction based on subject characteristic data for these samples. These results suggest that a metabolic profile can be used to predict an OUD positive diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41398-021-01228-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862627PMC
February 2021

A novel ternary heterogeneous TiO/BiVO/NaY-Zeolite nanocomposite for photocatalytic degradation of microcystin-leucine arginine (MC-LR) under visible light.

Ecotoxicol Environ Saf 2021 Mar 8;210:111862. Epub 2021 Jan 8.

Department of Environmental Health Engineering, Maragheh University of Medical Sciences, Maragheh, Iran. Electronic address:

Microcystin-leucine arginine (MC-LR) is a carcinogenic toxin, produced by cyanobacteria. The release of this toxin into drinking water sources can threaten public health and environmental safety. Therefore, effective MC-LR removal from water resources is necessary. In the present study, the hydrothermal method was used to synthesize a novel ternary BiVO/TiO/NaY-Zeolite (B/T/N-Z) nanocomposite for MC-LR degradation under visible light. FESEM, FTIR, XRD, and DRS were performed for characterizing the nanocomposite structure. Also, the Response Surface Methodology (RSM) was applied to determine the impact of catalyst dosage, pH, and contact time on the MC-LR removal. High-performance liquid chromatography was performed to measure the MC-LR concentration. Based on the results, independent parameters, including contact time, catalyst dosage, and pH, significantly affected the MC-LR removal (P < 0.05). In other words, increasing the contact time, catalyst dosage, and acidic pH had positive effects on MC-LR removal. Among these variables, the catalyst dosage, with the mean square and F-value of 1041.37 and 162.84, respectively, had the greatest effect on the MC-LR removal efficiency. Apart from the interaction between the catalyst dosage and contact time, the interaction effects of other parameters were not significant. Also, the maximum MC-LR removal efficiency was 99.88% under optimal conditions (contact time = 120 min, catalyst dosage = 1 g/L, and pH = 5). According to the results, the B/T/N-Z nanocomposite, as a novel and effective photocatalyst could be used to degrade MC-LR from polluted water.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2020.111862DOI Listing
March 2021

Untargeted Metabolomics: Biochemical Perturbations in Golestan Cohort Study Opium Users Inform Intervention Strategies.

Front Nutr 2020 22;7:584585. Epub 2020 Dec 22.

Department of Nutrition, Nutrition Research Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

Over 50 million people worldwide are estimated to use opioids, of which ~30 million use opiates (opium and its derivatives). Use of opiates has been associated with a variety of adverse complications such as neurological and behavioral outcomes, addiction, cancers, diabetes, and cardiovascular disease. While it is well known that opiates exert their neurobiological effects through binding with mu, kappa, and delta receptors to exert analgesic and sedative effects, mechanistic links to other health effects are not well understood. Our study focuses on the identification of biochemical perturbations in Golestan Cohort Study (GCS) opium users. We used untargeted metabolomics to evaluate the metabolic profiles of 218 opium users and 80 non-users participating in the GCS. Urine samples were obtained from adult (age 40-75) opium users living in the Golestan Province of Iran. Untargeted analysis of urine was conducted using a UPLC-Q-Exactive HFx Mass Spectrometry and a 700 MHz NMR Spectrometry. These GCS opium users had a significantly higher intake of tobacco and alcohol and a significantly decreased BMI compared with non-users. Metabolites derived from opium (codeine, morphine, and related glucuronides), nicotine, and curing or combustion of plant material were increased in opium users compared with non-users. Endogenous compounds which differentiated the opium users and non-users largely included vitamins and co-factors, metabolites involved in neurotransmission, Kreb's cycle, purine metabolism, central carbon metabolism, histone modification, and acetylation. Our study reveals biochemical perturbations in GCS opium users that are important to the development of intervention strategies to mitigate against the development of adverse effects of substance abuse.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnut.2020.584585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783045PMC
December 2020

Existing antiviral options against SARS-CoV-2 replication in COVID-19 patients.

Future Microbiol 2020 12 6;15:1747-1758. Epub 2021 Jan 6.

Department of Nutrition, Nutrition Research Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 28081, USA.

COVID-19 caused by SARS-CoV-2, is an international concern. This infection requires urgent efforts to develop new antiviral compounds. To date, no specific drug in controlling this disease has been identified. Developing the new treatment is usually time consuming, therefore using the repurposing broad-spectrum antiviral drugs could be an effective strategy to respond immediately. In this review, a number of broad-spectrum antivirals with potential efficacy to inhibit the virus replication via targeting the virus spike protein (S protein), RNA-dependent RNA polymerase (RdRp), 3-chymotrypsin-like protease (3CLpro) and papain-like protease (PLpro) that are critical in the pathogenesis and life cycle of coronavirus, have been evaluated as possible treatment options against SARS-CoV-2 in COVID-19 patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2217/fmb-2020-0120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789744PMC
December 2020

Clinical characteristics, outcomes, and risk factors for mortality in hospitalized patients with COVID-19 and cancer history: a propensity score-matched study.

Infect Agent Cancer 2020 Dec 17;15(1):74. Epub 2020 Dec 17.

Internal Medicine, Digestive Diseases Research Center, Digestive Diseases Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Kargar Shomali Avenue, Tehran, Iran.

Background: COVID-19 has caused great concern for patients with underlying medical conditions. We aimed to determine the prognosis of patients with current or previous cancer with either a PCR-confirmed COVID-19 infection or a probable diagnosis according to chest CT scan.

Methods: We conducted a case control study in a referral hospital on confirmed COVID-19 adult patients with and without a history of cancer from February to April, 2020. Patients were matched according to age, gender, and underlying diseases including ischemic heart disease (IHD), diabetes mellitus (DM), and hypertension (HTN). Demographic features, clinical data, comorbidities, symptoms, vital signs, laboratory findings, and chest computed tomography (CT) images have been extracted from patients' medical records. Multivariable logistic regression was used to estimate odd ratios and 95% confidence intervals of each factor of interest with outcomes.

Results: Fifty-three confirmed COVID-19 patients with history of cancer were recruited and compared with 106 non-cancerous COVID-19 patients as controls. Male to female ratio was 1.33 and 45% were older than 65. Dyspnea and fever were the most common presenting symptoms in our population with 57.86 and 52.83% respectively. Moreover, dyspnea was significantly associated with an increased rate of mortality in the cancer subgroup (p = 0.013). Twenty-six patients (49%) survived among the cancer group while 89 patients (84%) survived in control (p = 0.000). in cancer group, patients with hematologic cancer had 63% mortality while patients with solid tumors had 37%. multivariate analysis model for survival prediction showed that history of cancer, impaired consciousness level, tachypnea, tachycardia, leukocytosis and thrombocytopenia were associated with an increased risk of death.

Conclusion: In our study, cancer increased the mortality rate and hospital stay of COVID-19 patients and this effect remains significant after adjustment of confounders. Compared to solid tumors, hematologic malignancies have been associated with worse consequences and higher mortality rate. Clinical and para-clinical indicators were not appropriate to predict death in these patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13027-020-00339-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745169PMC
December 2020

Expression of Main Toll-Like Receptors in Patients with Different Types of Colorectal Polyps and Their Relationship with Gut Microbiota.

Int J Mol Sci 2020 Nov 26;21(23). Epub 2020 Nov 26.

Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran 1985717411, Iran.

Abnormal activation of Toll-like receptor (TLRs) signaling can result in colon cancer development. The aim of this study was to investigate the expression of important TLRs in different histological types of colorectal polyps and evaluate their relationship with intestinal microbiota. The expression levels of TLR2, 3, 4, and 5 were analyzed in intestinal biopsy specimens of 21 hyperplastic polyp (HP), 16 sessile serrated adenoma (SSA), 29 tubular adenoma (TA), 21 villous/tubulovillous (VP/TVP) cases, and 31 normal controls. In addition, selected gut bacteria including , , Enterotoxigenic (ETBF), , spp., spp., spp., and spp. were quantified in fecal samples using absolute qRT PCR, and, finally, the association between TLRs and these gut microbiota- was evaluated by Spearman's correlation coefficient. Higher expression of TLR2 and TLR4 in VP/TVP and TA, and lower expression levels of TLR3 and TLR5 in all type of polyps were observed. The differences in TLR expression patterns was not only dependent on the histology, location, size, and dysplasia grade of polyps but also related to the intestinal microbiota patterns. TLR2 and TLR4 expression was directly associated with the , , , and inversely to , , and quantity. Furthermore, TLR3 and TLR5 expression was directly associated with , , and quantity. Our results suggest a possible critical role of TLRs during colorectal polyp progression. An abnormal regulation of TLRs in relation to gut microbial quantity may contribute to carcinogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms21238968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7729598PMC
November 2020

The Khuzestan Comprehensive Health Study (KCHS): Methodology and Profile of Participants.

Arch Iran Med 2020 10 1;23(10):653-657. Epub 2020 Oct 1.

Liver and Pancreatobiliary Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.

Background: Non-communicable diseases (NCDs) are the leading cause of death worldwide, with a disproportionally rising burden among low- and middle-income populations. While preventable risk factors highly contribute to this burden, population-based studies assessing these factors and the health status of these populations, are scarce.

Methods: The Khuzestan Comprehensive Health Study (KCHS)-a cross sectional study-was conducted between 2016-2019, including 30,506 Iranians aged 20 to 65 years, from 27 counties of Khuzestan province, southwest of Iran. KCHS aimed to provide a comprehensive health overview by investigating the prevalence and risk factors of NCDs and psychological disorders, along with viral hepatitis as a common communicable disease. Upon registration, 15 mL of blood and anthropometric measurements were obtained from participants. Afterwards, several interviewer-administered questionnaires were completed to gather data on demographics, socioeconomic status, sleep quality, physical activity, lifestyle habits, nutrition, and medical history.

Results: The mean ± SD age of participants was 41.7 ± 11.9 years. The majority were female (64.3%), of the Arab ethnicity (49%), married (83%), and urban residents (73.1%). About 70% had an educational level below high school diploma. Overall, 10.8%, 5.2%, and 2.8% of participants had used cigarettes, hookah, and drugs at least once in their lifetime, respectively. While body mass index and serum cholesterol levels were higher in females, blood pressure was higher in males (<0.001).

Conclusion: KCHS assessed many aspects of health in the Khuzestan province. In addition to develop a biobank along with a comprehensive dataset, KCHS will serve as a valuable infrastructure for future research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.34172/aim.2020.82DOI Listing
October 2020

Exhaled breath condensate as a potential specimen for diagnosing COVID-19.

Bioanalysis 2020 09 15;12(17):1195-1197. Epub 2020 Apr 15.

Pharmaceutical Analysis Research Center & Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4155/bio-2020-0083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370802PMC
September 2020

Effects of doping zinc oxide nanoparticles with transition metals (Ag, Cu, Mn) on photocatalytic degradation of Direct Blue 15 dye under UV and visible light irradiation.

J Environ Health Sci Eng 2019 Jun 1;17(1):479-492. Epub 2019 Apr 1.

5Department of Water and Health, Faculty of Life Sciences, Jagadguru Sri Shivarathreeshwara University, Sri Shivarathreeshwara Nagara, Mysuru, Karnataka 570015 India.

Background: Azo dyes represent the most commonly used group of dyes in the textile industry. These organic dyes are mainly resistant to biodegradation and may exhibit toxic and carcinogenic properties. The purpose of this study was to investigate the effects of doping zinc oxide (ZnO) nanoparticles (NPs) with transition metals (silver, manganese, and copper) on the photocatalytic efficiency of ZnO NPs in the removal of Direct Blue 15 dye from aqueous environments under ultraviolet (UV) radiation and visible light irradiation.

Methods: One or two metals were used for doping the NPs. In total, seven types of undoped and transition metal-doped NPs were synthesized using the thermal solvent method with ZnO precursors and transition metal salts. The characteristics of the synthesized NPs were determined based on the scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), atomic force microscopy (AFM), and zeta potential measurements.

Results: The produced ZnO NPs did not exhibit any particular photocatalytic activities under UV radiation and visible light irradiation. The highest removal efficiency under UV radiation was about 74% in the presence of silver-doped ZnO NPs, while the maximum efficiency under visible light was 70% in the presence of copper-doped ZnO NPs. The lowest removal efficiency was related to pure ZnO, which was 18.4% and 14.6% under UV and visible light irradiation, respectively. Although the efficiency of dye removal under visible light was not high compared to UV radiation, this efficiency was noteworthy in terms of both practical and economic aspects since it was achieved without the presence of ultraviolet radiation.

Conclusions: The synthesis of transition metal-doped ZnO nanophotocatalysts (with one or two metals) under UV radiation or visible light irradiation could be used as an efficient and promising technology for the photocatalytic removal of Direct Blue 15 dye from aqueous environments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s40201-019-00366-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6582272PMC
June 2019

Assessment of the impact of different fecal storage protocols on the microbiota diversity and composition: a pilot study.

BMC Microbiol 2019 06 28;19(1):145. Epub 2019 Jun 28.

Digestive Oncology Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran.

Background: Fecal samples are currently the most commonly studied proxy for gut microbiota. The gold standard of sample handling and storage for microbiota analysis is maintaining the cold chain during sample transfer and immediate storage at - 80 °C. Gut microbiota studies in large-scale, population-based cohorts require a feasible sample collection protocol. We compared the effect of three different storage methods and mock shipment: immediate freezing at - 80 °C, in 95% ethanol stored at room temperature (RT) for 48 h, and on blood collection card stored at RT for 48 h, on the measured composition of fecal microbiota of eight healthy, female volunteers by sequencing the V4 region of the 16S rRNA gene on an Illumina MiSeq.

Results: Shared operational taxonomic units (OTUs) between different methods were 68 and 3% for OTUs > 0.01 and < 0.01% mean relative abundance within each group, respectively. α and β-diversity measures were not significantly impacted by different storage methods. With the exception of Actinobacteria, fecal microbiota profiles at the phylum level were not significantly affected by the storage method. Actinobacteria was significantly higher in samples collected on card compared to immediate freezing (1.6 ± 1.1% vs. 0.4 ± 0.2%, p = 0.005) mainly driven by expansion of Actinobacteria relative abundance in fecal samples stored on card in two individuals. There was no statistically significant difference at lower taxonomic levels tested.

Conclusion: Consistent results of the microbiota composition and structure for different storage methods were observed. Fecal collection on card could be a suitable alternative to immediate freezing for fecal microbiota analysis using 16S rRNA gene amplicon sequencing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12866-019-1519-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599303PMC
June 2019

Data on THMs concentration and spatial trend in water distribution network (a preliminary study in center of Iran).

MethodsX 2019 3;6:760-763. Epub 2019 Apr 3.

Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

The Trihalomethanes (THMs) formed due to a reaction between water disinfection chlorine and some natural organic matters, as chlorinated by products. The aim of this study was determination of THMs values and spatial trend in Yazd city water distribution network, in center of Iran. Sampling of tap water was done in two autumn and winter seasons. The THMs value were measured by using a gas chromatograph-mass spectrometer (GC/MS), Agilent Company 6890 N. The spatial analysis of THMs values was carried out using ArcGIS 10.1 to show the spatial spreading. The Kriging method was used to draw distribution maps. Using the Kriging method to illustrate the difference or precision of forecasts is relatively easy compared to the other interpolation methods. Also, the acceptable level of % RMSE (Root mean square error) was calculated for Kriging method (% RMSE > 40). Thus, this protocol as integrated between data and geraphic could easily used for reporting of THMs level in studies of water distribution network. Finally, the maximum THMs value were obtained lower than USEPA and WHO guidelines for drinking water (THMs < 40 ppb).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mex.2019.03.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6462497PMC
April 2019

Impact of Molecular Partitioning and Partial Equilibration on the Estimation of Diffusion Coefficients from Release Experiments.

Langmuir 2019 04 12;35(16):5663-5671. Epub 2019 Apr 12.

Food and Soft Materials Science, Department of Health Science & Technology, Institute of Food, Nutrition & Health , ETH Zurich , Schmelzbergstrasse 9 , CH-8092 Zurich , Switzerland.

The present work addresses the effect of partial equilibration and molecular partitioning on the interpretation of release experiments. In this regard, it is shown how release profiles and the values of extracted transport parameters are affected by the time protocol chosen for sample collection by considering a series of experiments where the latter is systematically varied. Caffeine is investigated as a main model drug because of its similar affinity for water and lipids, while monolinolein-based lipid cubic phases are chosen as host matrices because of their wide employment in release studies. Our findings point to a progressive decline in diffusion rate upon increasing the time step, that is, the gap in time between two consecutive pickups, which is a signature of increasing equilibration of caffeine concentration between the lipidic mesophase and the water phase. Furthermore, the amount of released molecules at the first pickup displays negligible changes for large time steps, indicating complete equilibration in such cases. A model is introduced based on Fick's diffusion which goes beyond the assumption of perfect-sink conditions, a common feature of the typical theoretical approaches hitherto developed. The model is shown to account quantitatively for the experimental data and is subsequently employed to clarify the interplay of the adopted release protocol with the various transport parameters in determining the final outcome of the release process. Particularly, two additional molecular drugs are considered, namely glucose and proflavine, which are, respectively, more hydrophilic and hydrophobic than caffeine, thus allowing elucidating the role of molecular partitioning.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.9b00510DOI Listing
April 2019

The interplay of channel geometry and molecular features determines diffusion in lipidic cubic phases.

J Chem Phys 2019 Mar;150(9):094901

Department of Health Sciences and Technology, ETH Zurich, CH-8092 Zurich, Switzerland.

The transport behavior of inverse bicontinuous cubic phases is experimentally investigated as the combined outcome of solute molecular structure and geometrical details of the confining symmetry. Molecular diffusion is discussed in relation to curvature, bottlenecks, and interfacial properties of each cubic phase. Point-like molecules show faster diffusion across the double diamond (Pn3¯m) symmetry, while unfolded macromolecules display better performance inside the double primitive (Im3¯m) cubic phase. The former observation is in agreement with previous simulation work, whereas the latter indicates that dedicated theory needs to be developed for diffusing polymers. Furthermore, the effect of electrostatic interactions is assessed by a study of diffusion of nanoparticles and is rationalized via a combination of simulations and theoretical considerations as the result of a competition between water mobility and geometrical features of the channel.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1063/1.5080929DOI Listing
March 2019

Decolorization and biodegradation of reactive Red 198 Azo dye by a new Enterococcus faecalis-Klebsiella variicola bacterial consortium isolated from textile wastewater sludge.

World J Microbiol Biotechnol 2019 Feb 9;35(3):38. Epub 2019 Feb 9.

Environmental Science and Technology Research Center, Department of Environmental Health Engineering, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

The present study investigated biodegradation and removal of Reactive Red 198 (RR198) dye from aqueous environments using a new bacterial consortium isolated from textile wastewater sludge on laboratory scale via batch study. Two bacterial species, Enterococcus faecalis (EF) and Klebsiella variicola (KV), were identified after isolation, through biochemical assays, Polymerase chain reaction (PCR), and 16S rRNA gene sequencing. To determine their ability to biodegrade RR198 dye, physicochemical parameters, including bacterial concentration, time, pH, and temperature, were tested; the results showed that the best conditions included a bacterial concentration of 3.5 mL × 10 cells/mL and incubation time of 72 h. Under such conditions, the removal efficiency of RR198 dye at an initial concentration of 10-25 mg/L was more than 98%; however, for concentrations of 50, 75, and 100 mg/L, removal efficiency was reduced to 55.62%, 25.82%, and 15.42%, respectively (p = 0.005). The highest removal efficiency occurred at pH 8.0, reaching 99.26% after 72 h of incubation. With increasing the incubation temperature from 25 °C to 37 °C, removal efficiency increased from 71.71 to 99.26% after 72 h of incubation, and increasing the temperature from 37 to 45 °C, the removal efficiency was reduced (p ≤ 0.001). Therefore, the EF-KV bacterial consortium can be used for efficient removal of RR198 dye from textile effluent.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11274-019-2608-yDOI Listing
February 2019

Toxicity evaluation and management of co-composting pistachio wastes combined with cattle manure and municipal sewage sludge.

Ecotoxicol Environ Saf 2019 Apr 17;171:798-804. Epub 2019 Jan 17.

Environmental Science and Technology Research Center, Department of Environmental Health Engineering, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Electronic address:

To manage the pistachio de-hulling waste (PW), investigated the co-composting process using a mixture of PW and cattle manure (CM) (5.5:10 dry weight) as well as PW and municipal dewatering sewage sludge (DSS) (1:10 dry weight) at the laboratory scale for 60 days. Compost toxicity was evaluated using the seed germination index (GI). The maximum temperatures in co-composting processes of PW+CM (51.9 °C) and PW+DSS (49.9 °C) were reported on the seventh day. The increase of temperature was higher in PW+CM and remained in the thermophilic phase for five days. In both reactors, the pH rates decreased, increased, and finally remained neutral. The C/N ratio decreased in both reactors, but the reduction rate was faster in the PW+DSS reactor. Sodium (Na%) and potassium (K%) contents were increased, while the concentrations of Cu, Zn, Fe, and Mn were decreased during the processes. The numbers of parasite eggs in the final composts of the reactors containing PW+CM and PW+DSS were zero and 8 Number/4gDW, respectively. The amounts of Salmonella were zero in the final products of reactors containing PW+CM and PW+DSS. The results of GI showed that the final compost of PW+CM process was not toxic for the plants, whereas the PW+DDS final compost was toxic. In conclusion, the co-compost product of the PW+CM reactor had higher quality than the PW+DSS reactor. So, it is suitable for PW management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2019.01.056DOI Listing
April 2019

Dataset on comparing the corrosion indices of alum and ferric chloride coagulants with phosphate dose elevation.

Data Brief 2018 Oct 9;20:281-288. Epub 2018 Aug 9.

Department of Environmental Health Engineering, Faculty of Health, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

The aim of this data was to assess and compare the corrosion indices of alum and ferric chloride coagulants in conventional coagulation process of water with elevation of phosphate dose. After preparing synthetic water samples, jar experiments were performed with elevation of different phosphate doses using alum and ferric chloride coagulants. Then, corrosion indices of Ryznar and Langelier of water samples were calculated. The results indicated that the values of Ryznar and Langelier index in the experimental samples were 7 and less than zero, and the water conditions were under saturated. The corrosion and precipitation indices indicated that the water samples can be considered as corrosive waters.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.dib.2018.08.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6106702PMC
October 2018

Potential Correlation Between Circulating Fetuin-A and Pentraxin-3 With Biochemical Parameters of Calcification in Hemodialysis Patients.

Arch Iran Med 2017 Dec 1;20(12):752-755. Epub 2017 Dec 1.

School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.

Background: The strong correlation between vascular calcification and cardiovascular risk, which is a major cause of mortality in hemodialysis (HD) patients, has been well established. Fetuin-A is an inhibitor of vascular calcification, and pentraxin 3 (PTX3) is produced at the site of inflammation, which is associated with cardiovascular disease (CVD). The main purpose of this study was evaluating the correlation between fetuin-A and PTX3 with some biochemical parameters effective upon vascular calcification in HD patients.

Methods: We included 84 HD patients and 84 healthy controls matched for age, gender, and body mass index (BMI) in this study. Blood samples were drawn from all subjects and the serum levels of creatinine, urea, albumin, calcium (Ca), phosphorus (P), lowdensity lipoprotein cholesterol (LDL-C), parathyroid hormone, fetuin-A, high sensitive C-reactive protein, and PTX3 were measured by biochemical methods.

Results: We found that the serum levels of PTX3, C-reactive protein (CRP), parathyroid hormone (PTH), Ca, and P in the patient group were significantly higher than the control group but the serum levels of fetuin-A and albumin were significantly lower in the patient group. Also, fetuin-A had a significant correlation with high sensitive CRP (hs-CRP) as well as duration of dialysis. In addition, it was shown that the correlation between PTX3 and PTH was significant only in the patient group.

Conclusion: In this study, increased PTX3 and decreased fetuin-A levels were observed in the HD patients. According to our results, these 2 parameters may potentially serve as suitable markers for inflammation and prediction of vascular complications in these patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
December 2017

Rheology of Ultraswollen Bicontinuous Lipidic Cubic Phases.

Langmuir 2018 05 20;34(17):5052-5059. Epub 2018 Apr 20.

Department of Health Science & Technology , ETH Zürich , Schmelzbergstrasse 9, LFO, E23 , 8092 Zürich , Switzerland.

Rheological studies of liquid crystalline systems based on monopalmitolein and 5 or 8% of 1,2 distearoylphosphatidylglycerol are reported. Such cubic phases have been shown to possess unusually large water channels because of their ability of accommodating up to 80 wt % of water, a feature that renders these systems suitable for crystallizing membrane proteins with large extracellular domains. Their mechanical properties are supposed to be substantially different from those of traditional cubic phases. Rheological measurements were carried out on cubic phases of both Pn3 m and Ia3 d symmetries. It was verified that these ultraswollen cubic phases are less rigid than the normal cubic phases, with the Pn3 m being softer that the Ia3 d ones. Furthermore, for the Pn3 m case, the longest relaxation time is shown to decrease logarithmically with increasing surface area per unit volume, proving the critical role of the density of interfaces in establishing the macroscopic viscoelastic properties of the bicontinuous cubic phases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.8b00737DOI Listing
May 2018

Using Metabolomics to Investigate Biomarkers of Drug Addiction.

Trends Mol Med 2018 02 31;24(2):197-205. Epub 2018 Jan 31.

Department of Nutrition, Nutrition Research Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address:

Drug addiction has been associated with an increased risk for cancer, psychological complications, heart, liver, and lung disease, as well as infection. While genes have been identified that can mark individuals at risk for substance abuse, the initiation step of addiction is attributed to persistent metabolic disruptions occurring following the first instance of narcotic drug use. Advances in analytical technologies can enable the detection of thousands of signals in body fluids and excreta that can be used to define biochemical profiles of addiction. Today, these approaches hold promise for determining how exposure to drugs, in the absence or presence of other environmentally relevant factors, can impact human metabolism. We posit that these can lead to candidate biomarkers of drug dependence, treatment, withdrawal, or relapse.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.molmed.2017.12.005DOI Listing
February 2018

Gut microbiota, epigenetic modification and colorectal cancer.

Iran J Microbiol 2017 Apr;9(2):55-63

Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Micro-organisms contain 90% of cells in human body and trillions foreign genes versus less than 30 thousand of their own. The human colon host various species of microorganisms, appraised at more than 10 microbiota and contained of over a thousand species. Although each one's profile is separable, the relative abundance and distribution of bacterial species is the same between healthy ones, causing conservation of each person's overall health. Germline DNA mutations have been attributed to the less than 5% of CRC occurrence while more than 90% is associated with the epigenetic regulation. The most ubiquitous environmental factor in epigenetic modification is gut microbiota. Disruptive changes in the gut microbiome strongly contributed to the improvement of colorectal cancer. Gut microbiota may play critical role in progression of CRC via their metabolite or their structural component interacting with host intestinal epithelial cell (IEC). Herein we discuss the mechanism of epigenetic modification and its implication in CRC development, progression even metastasis by gut microbiota induction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5715278PMC
April 2017

Providing an imputation algorithm for missing values of longitudinal data using Cuckoo search algorithm: A case study on cervical dystonia.

Electron Physician 2017 Jun 25;9(6):4648-4654. Epub 2017 Jun 25.

Ph.D., Associate Professor, Faculty of Mathematical Sciences, Department of Applied Mathematics, Ferdowsi University of Mashhad, Mashhad, Iran.

Background: Missing values in data are found in a large number of studies in the field of medical sciences, especially longitudinal ones, in which repeated measurements are taken from each person during the study. In this regard, several statistical endeavors have been performed on the concepts, issues, and theoretical methods during the past few decades.

Methods: Herein, we focused on the missing data related to patients excluded from longitudinal studies. To this end, two statistical parameters of similarity and correlation coefficient were employed. In addition, metaheuristic algorithms were applied to achieve an optimal solution. The selected metaheuristic algorithm, which has a great search functionality, was the Cuckoo search algorithm.

Results: Profiles of subjects with cervical dystonia (CD) were used to evaluate the proposed model after applying missingness. It was concluded that the algorithm used in this study had a higher accuracy (98.48%), compared with similar approaches.

Conclusion: Concomitant use of similar parameters and correlation coefficients led to a significant increase in accuracy of missing data imputation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.19082/4648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5557148PMC
June 2017

Diffusion of Polymers through Periodic Networks of Lipid-Based Nanochannels.

Langmuir 2017 04 28;33(14):3491-3498. Epub 2017 Mar 28.

Department of Health Sciences & Technology, ETH Zurich , Schmelzbergstrasse 9, CH-8092 Zurich, Switzerland.

We present an experimental investigation of the diffusion of unfolded polymers in the triply-periodic water-channel network of inverse bicontinuous cubic phases. Depending on the chain size, our results indicate the presence of two different dynamical regimes corresponding to Zimm and Rouse diffusion. We support our findings by scaling arguments based on a combination of blob and effective-medium theories and suggest the presence of a third regime where dynamics is driven by reptation. Our experimental results also show an increasing behavior of the partition coefficient as a function of the polymer molecular weight, indicative of a reduction in the conformational degrees of freedom induced by the confinement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.7b00437DOI Listing
April 2017

Expression Analysis of Previously Verified Fecal and Plasma Dow-regulated MicroRNAs (miR-4478, 1295-3p, 142-3p and 26a-5p), in FFPE Tissue Samples of CRC Patients.

Arch Iran Med 2017 Feb;20(2):92-95

Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.

Background: Colorectal cancer (CRC) is one of the most common causes of cancer-related mortality worldwide. Early diagnosis of this neoplasm is critical and may reduce patients' mortality. MicroRNAs are small non-coding RNA molecules whose expression pattern can be altered in various diseases such as CRC.

Methods: In this study, we evaluated the expression levels of miR-142-3p, miR-26a-5p (their reduced expression in plasma samples of CRC patients was previously confirmed), miR-4478 and miR-1295-3p (their reduced expression in stool samples of CRC patients was previously confirmed) in tissue samples of CRC patients in comparison to healthy subjects. To achieve this purpose, total RNA including small RNA was extracted from 53 CRC and 35 normal subjects' Formalin-fixed, Paraffin-embedded (FFPE) tissue samples using the miRNeasy FFPE Mini Kit. The expression levels of these four selected miRNAs were measured using quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR).

Results: We found that the expression levels of miR-4478 and miR-1295b-3p (two previously down-regulated fecal miRNAs) were significantly decreased in FFPE samples of CRC patients compared to healthy controls. On the other hand, no significant differences were seen in expression levels of miR-142-3p and miR-26a-5p (two previously down-regulated circulating miRNAs) in FFPE samples between these two groups.

Conclusion: Regarding current findings, it may be concluded that to diagnose CRC patients based on the miRNAs approach, stool samples are more likely preferable to plasma samples; nevertheless, additional studies with more samples are needed to confirm the results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/0172002/AIM.006DOI Listing
February 2017

Investigation of MicroRNA-21 Expression Levels in Serum and Stool as a Potential Non-Invasive Biomarker for Diagnosis of Colorectal Cancer.

Iran Biomed J 2017 03 19;21(2):106-13. Epub 2016 Jul 19.

Dept. of Molecular Medicine and Genetics, Research Center for Molecular Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Background: Most cancer studies focus on exploring non-invasive biomarkers for cancer detection. In the present study, we sought to investigate the expression level of microRNA-21 (miR-21), as a potential diagnostic marker, in serum and stool samples from 40 patients with colorectal cancer (CRC) and 40 healthy controls.

Methods: Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 in serum and stool. At the same time, the sensitivity and specificity of this marker was evaluated by receiver operating characteristic (ROC) curve analysis.

Results: miR-21 expression levels of serum and stool were up-regulated 12.1 (P<0.05, 95% CI: 5.774-34.045) and 10.0 (P<0.05, 95% CI: 0.351-16.260) times in CRC patients, respectively, when compared to the control group. The sensitivity and specificity of miR-21 was found to be 86.05% and 72.97%, respectively (an area under the ROC curve [AUC] of 0.783). The stool miR-21 level in CRC patients was much higher than that in the healthy controls, showing a sensitivity of 86.05% and a specificity of 81.08% (AUC: 0.829). The expression level of miR-21 in stool was able to significantly distinguish CRC tumor, node, metastasis stages III-IV from stages I-II, with a sensitivity and specificity of 88.1% and 81.6%, respectively (AUC: 0.872).

Conclusion: The results of this study indicated that miR-21 expression levels in serum and stool can be considered as a potential diagnostic biomarker for the diagnosis of CRC patients. However, more studies are required to confirm the validity of miR-21 as a valuable non-invasive diagnostic tool for CRC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18869/acadpub.ibj.21.2.106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5274709PMC
March 2017

Inverse Association of Plasma Level of Glutathione Peroxidase with Liver Fibrosis in Chronic Hepatitis B: Potential Role of Iron.

Middle East J Dig Dis 2016 Apr;8(2):122-30

Liver and Pancreatobiliary Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.

BACKGROUND Oxidative stress has a major pathogenic role for liver damage following chronic hepatitis B. Glutathione peroxidase (Gpx) is necessary in oxidative state mechanism that is generally down-regulated by Hepatitis B virus (HBV) infection. On the other hand, disorders of iron homeostasis have been found out in HBV infected patients. Therefore, the objective of this study was to assess the interplay of Gpx and serum iron on clinical and virological features of patients with chronic HBV infection. METHODS One hundred and fifty adult, treatment-naïve, patients with chronic hepatitis B were randomly designated from an ongoing cohort of patients with HBV. Plasma Gpx1 concentration and HBV DNA quantity were measured. Liver stiffness was measured by transient elastography. RESULTS Serum iron had a positive association with HBV DNA count in the total population. Serum iron was not associated with liver stiffness. However, HBV DNA was significantly associated with liver stiffness only in male patients. Serum Gpx was inversely associated with liver stiffness. Serum iron and Gpx had indirect effects on liver stiffness via HBV DNA count. We observed dissimilar effects of serum iron on HBV DNA and Gpx on liver stiffness in male and female patients. CONCLUSION We identified interplay of serum iron and Gpx1 in relation to level of liver fibrosis in patients with chronic hepatitis B. Our results propose that oxidative stress and serum iron are differentially implicated in the progression of chronic hepatitis B in male and female patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.15171/mejdd.2016.17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885611PMC
April 2016

Driver Gene Mutations in Stools of Colorectal Carcinoma Patients Detected by Targeted Next-Generation Sequencing.

J Mol Diagn 2016 07 4;18(4):471-9. Epub 2016 May 4.

Department of Pathology, Faculty of Medicine, University of Helsinki, Helsinki, Finland. Electronic address:

Detection of driver gene mutations in stool DNA represents a promising noninvasive approach for screening colorectal cancer (CRC). Amplicon-based next-generation sequencing (NGS) is a good option to study mutations in many cancer genes simultaneously and from a low amount of DNA. Our aim was to assess the feasibility of identifying mutations in 22 cancer driver genes with Ion Torrent technology in stool DNA from a series of 65 CRC patients. The assay was successful in 80% of stool DNA samples. NGS results showed 83 mutations in cancer driver genes, 29 hotspot and 54 novel mutations. One to five genes were mutated in 75% of cases. TP53, KRAS, FBXW7, and SMAD4 were the top mutated genes, consistent with previous studies. Of samples with mutations, 54% presented concomitant mutations in different genes. Phosphatidylinositol 3-kinase/mitogen-activated protein kinase pathway genes were mutated in 70% of samples, with 58% having alterations in KRAS, NRAS, or BRAF. Because mutations in these genes can compromise the efficacy of epidermal growth factor receptor blockade in CRC patients, identifying mutations that confer resistance to some targeted treatments may be useful to guide therapeutic decisions. In conclusion, the data presented herein show that NGS procedures on stool DNA represent a promising tool to detect genetic mutations that could be used in the future for diagnosis, monitoring, or treating CRC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jmoldx.2016.01.008DOI Listing
July 2016

Simultaneous Underexpression of let-7a-5p and let-7f-5p microRNAs in Plasma and Stool Samples from Early Stage Colorectal Carcinoma.

Biomark Cancer 2015 12;7(Suppl 1):39-48. Epub 2016 Jan 12.

Department of Pathology, Faculty of Medicine, The University of Helsinki, Helsinki, Finland.

Colorectal cancer (CRC) is the third most common malignancy and the second most common cause of cancer death worldwide. Early detection of CRC can improve patient survival rates; thus, the identification of noninvasive diagnostic markers is urgently needed. MicroRNAs (miRNAs) have extensive potential to diagnose several diseases, including cancer. In this study, we compared the expression pattern of miRNAs from plasma and stool samples of patients with early stages of CRC (I, II) with that of healthy subjects. We performed miRNA profiling using microarrays on plasma and stool samples of eight patients with CRC and four healthy subjects. Seven miRNAs were found to be underexpressed in both plasma and stool samples of patients with CRC versus healthy subjects. Then, we aimed to verify two out of these seven differentially expressed miRNAs (let-7a-5p and let-7f-5p) by quantitative reverse transcriptase polymerase chain reaction on a larger set of plasma and stool samples of 51 patients with CRC and 26 healthy subjects. We confirmed the results of microarray analysis since their expression was significantly lower in stool and plasma samples of patients with CRC. Moreover, receiver operating characteristic curve analysis demonstrated that fecal let-7f expression levels have significant sensitivity and specificity to distinguish between patients with CRC and healthy subjects. In conclusion, if the results are confirmed in larger series of patients, underexpressed let-7a-5p and let-7f-5p miRNAs in both plasma and stool samples of patients with CRC may serve potentially as noninvasive molecular biomarkers for the early detection of CRC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4137/BIC.S25252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711391PMC
January 2016

Downregulation of Plasma MiR-142-3p and MiR-26a-5p in Patients With Colorectal Carcinoma.

Iran J Cancer Prev 2015 May 22;8(3):e2329. Epub 2015 May 22.

Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IR Iran.

Background: Colorectal cancer is one of the most commonly diagnosed cancers and cancer- related death worldwide. Identification of new specific biomarkers could be helpful to detection of this malignancy. Altered plasma microRNA expression has been identified in many cancers, including colorectal cancer.

Objectives: The main objective of this study was to identify the circulating microRNAs with the most expression changes in colorectal cancer patients compared with neoplasm free healthy individuals.

Materials And Methods: MicroRNA expression profiling was performed on plasma samples of 37 colorectal cancer patients and 8 normal subjects using microRNA microarray. Quantitative real-time reverse transcription polymerase chain reaction was used to validate the two selected altered microR NAs. Plasma samples from 61 colorectal cancer patients and 24 normal subjects were used in our validation study.

Results: In profiling study we found a panel of six plasma microRNAs with significant downregulation. MicroRNA-142-3p and microRNA-26a-5p were selected and validated by polymerase chain reaction. Our results demonstrated that expression levels of plasma microRNA-142-3p and microRNA-26a-5p were significantly downregulated in patients with colorectal cancer when compared to control group.

Conclusions: Our findings suggest that downregulation of plasma microRNA-142-3p and microRNA-26a-5p might serve as novel noninvasive biomarkers in the diagnosis of colorectal cancer, although more studies are needed to highlight the theoretical strengths.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.17795/ijcp2329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581368PMC
May 2015