Publications by authors named "Reyhaneh Sadat Saberi"

3 Publications

  • Page 1 of 1

A label-free electrochemical aptasensor for breast cancer cell detection based on a reduced graphene oxide-chitosan-gold nanoparticle composite.

Bioelectrochemistry 2021 Mar 26;140:107807. Epub 2021 Mar 26.

Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran. Electronic address:

Regarding the cancer fatal consequences, early detection and progression monitoring are the most vital issues in patients' treatment and mortality reduction. Therefore, there is a great demand for fast, inexpensive, and selective detection methods. Herein, a graphene-based aptasensor was designed for sensitive human breast cancer cell detection. A reduced graphene oxide-chitosan-gold nanoparticles composite was used as a biocompatible substrate for the receptor stabilization. The significant function of the aptamer on this composite is due to the synergistic effects of the components in improving the properties of the composite, including increasing the electrical conductivity and effective surface area. After the aptasensor incubation in MCF-7 cancer cells, the cell membrane proteins interacted specifically with the three dimensional-structure of the AS1411 aptamer, resulting in the cell capture on the aptasensor. The aptasensor fabrication steps were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. The higher cell concentrations concluded to the higher captured cells on the aptasensor which blocked the Ferro/Ferricyanide access to the sensor, causing increases in the charge transfer resistances. This aptasensor shows a linear relationship with the cell concentration logarithm, high selectivity, a wide linear range of 1 × 10-1 × 10 cells/mL, and a low detection limit of 4 cells/mL.
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http://dx.doi.org/10.1016/j.bioelechem.2021.107807DOI Listing
March 2021

Graphene-family materials in electrochemical aptasensors.

Anal Bioanal Chem 2021 Jan 17;413(3):673-699. Epub 2020 Sep 17.

East Sage Investigative Corporation, Isfahan Science and Technology Town, Isfahan, 8415683111, Iran.

The study of graphene-based carbon nanocomposites has remarkably increased in recent years. Functionalized graphene-based nanostructures, including graphene oxide and reduced graphene oxide, have great potential as new innovative electrode materials in the fabrication of novel electrochemical sensors. Electrochemical sensors based on aptamers attracted great attention because of their high sensitivity and selectivity, and simple instrumentation, as well as low production cost. Aptamers as a potent alternative to antibodies are functional nucleic acids with a high tendency to specific analytes. Electrochemical aptasensors show specific recognition ability for a wide range of analytes. Although aptamers are selected in vitro in contrast to antibodies, they are interesting due to advantages like high stability, easy chemical modifications, and the potential to be employed in nanostructured device fabrication or electrochemical sensing devices. Recently, new nanomaterials have shown a significant impact on the production of electrochemical sensors with high efficiency and performance. This review aims to give an outline of electrochemical aptasensors based on the graphene family materials and discuss the detection mechanism in this type of aptasensors. The present review summarizes some of the recent achievements in graphene-based aptasensors and includes their recent electroanalytical applications. Graphical Abstract Graphical Abstract.
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http://dx.doi.org/10.1007/s00216-020-02915-yDOI Listing
January 2021

Highly sensitive voltammetric determination of lamotrigine at highly oriented pyrolytic graphite electrode.

Bioelectrochemistry 2012 Apr 4;84:38-43. Epub 2011 Nov 4.

Department of Chemistry, Sharif University of Technology, Tehran 11155-9516, Iran.

The electrochemical behavior of lamotrigine (LMT) at the pyrolytic graphite electrode (PGE) is investigated in detail by the means of cyclic voltammetry. During the electrochemical reduction of LMT, an irreversible cathodic peak appeared. Cyclic voltammetric studies indicated that the reduction process has an irreversible and adsorption-like behavior. The observed reduction peak is attributed to a two-electron process referring to the reduction of azo group. The electrode showed an excellent electrochemical activity toward the electro-reduction of LMT, leading to a significant improvement in sensitivity as compared to the glassy carbon electrode. The results of electrochemical impedance spectroscopy and cyclic voltammetry showed that edge-plane pyrolytic graphite electrode has excellent electrochemical response properties toward LMTs with respect to glassy carbon electrode modified with carbon nanotubes. High sensitivity, low detection limit and very good repeatability together with excellent recovery make the electrode as a powerful devise for accurate determination of LMT in pharmaceutical and biological samples.
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http://dx.doi.org/10.1016/j.bioelechem.2011.10.008DOI Listing
April 2012