Publications by authors named "Ren Dong"

276 Publications

Maternal care in Mid-Cretaceous lagonomegopid spiders.

Proc Biol Sci 2021 Sep 15;288(1958):20211279. Epub 2021 Sep 15.

College of Life Sciences and Academy for Multidisciplinary Studies, Capital Normal University, 105 Xisanhuanbeilu, Haidian District, Beijing 100048, People's Republic of China.

Maternal care benefits the survival and fitness of offspring, often at a cost to the mother's future reproduction, and has evolved repeatedly throughout the animal kingdom. In extant spider species, this behaviour is very common and has different levels and diverse forms. However, evidence of maternal care in fossil spiders is quite rare. In this study, we describe four Mid-Cretaceous (approx. 99 Ma) amber specimens from northern Myanmar with an adult female, part of an egg sac and some spiderlings of the extinct family Lagonomegopidae preserved, which suggest that adult lagonomegopid females probably built and then guarded egg sacs in their retreats or nests, and the hatched spiderlings may have stayed together with their mother for some time. The new fossils represent early evidence of maternal care in fossil spiders, and enhance our understanding of the evolution of this behaviour.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1098/rspb.2021.1279DOI Listing
September 2021

Silencing ZIC2 abrogates tumorigenesis and anoikis resistance of non-small cell lung cancer cells by inhibiting Src/FAK signaling.

Mol Ther Oncolytics 2021 Sep 29;22:195-208. Epub 2021 May 29.

Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China.

Aberrant expression of the zinc finger protein (ZIC) family has been extensively reported to contribute to progression and metastasis in multiple human cancers. However, the functional roles and underlying mechanisms of ZIC2 in non-small cell lung cancer (NSCLC) are largely unknown. In this study, ZIC2 expression was evaluated using qRT-PCR, western blot, and immunohistochemistry, respectively. Animal experiments and functional assays were performed to investigate the role of ZIC2 in NSCLC. Luciferase assays and chromatin immunoprecipitation (ChIP) were carried out to explore the underlying target involved in the roles of ZIC2 in NSCLC. Here, we reported that ZIC2 was upregulated in NSCLC tissues, and high expression of ZIC2 predicted worse overall and progression-free survival of NSCLC patients. Silencing ZIC2 repressed tumorigenesis and reduced the anoikis resistance of NSCLC cells. Mechanical investigation further revealed that silencing ZIC2 transcriptionally inhibited Src expression and inactivated steroid receptor coactivator/focal adhesion kinase signaling, which further attenuated the anoikis resistance of NSCLC cells. Importantly, our results showed that the number of circulating tumor cells (CTCs) was positively correlated with ZIC2 expression in NSCLC patients. Collectively, our findings unravel a novel mechanism implicating ZIC2 in NSCLC, which will facilitate the development of anti-tumor strategies in NSCLC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.omto.2021.05.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424131PMC
September 2021

A Review of Hand-Arm Vibration Studies Conducted by US NIOSH since 2000.

Vibration 2021 Jun;4(2):482-528

Physical Effects Research Branch, Health Effects Laboratory Division (HELD), National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV 26505, USA.

Studies on hand-transmitted vibration exposure, biodynamic responses, and biological effects were conducted by researchers at the Health Effects Laboratory Division (HELD) of the National Institute for Occupational Safety and Health (NIOSH) during the last 20 years. These studies are systematically reviewed in this report, along with the identification of areas where additional research is needed. The majority of the studies cover the following aspects: (i) the methods and techniques for measuring hand-transmitted vibration exposure; (ii) vibration biodynamics of the hand-arm system and the quantification of vibration exposure; (iii) biological effects of hand-transmitted vibration exposure; (iv) measurements of vibration-induced health effects; (iv) quantification of influencing biomechanical effects; and (v) intervention methods and technologies for controlling hand-transmitted vibration exposure. The major findings of the studies are summarized and discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/vibration4020030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371562PMC
June 2021

Early Cretaceous mealybug herbivory on a laurel highlights the deep-time history of angiosperm-scale insect associations.

New Phytol 2021 Aug 11. Epub 2021 Aug 11.

College of Life Sciences and Academy for Multidisciplinary Studies, Capital Normal University, Beijing, 100048, China.

Insect fluid-feeding on fossil vascular plants is an inconspicuous and underappreciated mode of herbivory that can provide novel data on the evolution of deep-time ecological associations and indicate the host-plant preferences of ancient insect herbivores. Previous fossil studies have documented piercing-and-sucking herbivory but often are unable to identify culprit insect taxa. One line of evidence are punctures and scale-insect impression marks made by piercing-and-sucking insects that occasionally provide clues to the systematic identities and relationships of particular insect herbivores. We report here the earliest occurrences of piercing and sucking on early angiosperms as evidenced by scale insect covers, impression marks, punctures and body fossils - notably a mealybug - from the Lower Cretaceous Rose Creek Flora of the Dakota Formation (c. 103 Ma), in southeastern Nebraska, USA. The mealybug, two other scale insect taxa, and several distinctive damage types on laurel leaves and seed-plant stems at Rose Creek document a diverse guild of piercing-and-sucking insects on early angiosperms. The discovery of an Early Cretaceous female mealybug indicates an early herbivorous association with a laurel host. These data provide direct evidence for co-associations and possible coevolution of scale insects and their plant hosts during early angiosperm diversification.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.17672DOI Listing
August 2021

The first queen-worker association for Cretaceous Formicidae: the winged caste of .

Zookeys 2021 7;1048:69-78. Epub 2021 Jul 7.

College of Life Sciences and Academy for Multidisciplinary Studies, Capital Normal University, 105 Xisanhuanbeilu, Haidian District, Beijing 100048, China Capital Normal University Beijing China.

Two queen ant specimens, one alate and one dealate, from mid-Cretaceous (Late Albian-Early Cenomanian) Burmese amber are herein reported as belonging Dlussky, 1996. This is the first discovery and documentation of an alate queen in . Compared with workers of , alate and dealate queens are larger in body size, have smaller compound eyes, a longer antennal scape, more complex mandibles, and a relatively large-sized metasoma. It is hypothesized that these differences are due to caste differences.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3897/zookeys.1048.66920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280096PMC
July 2021

Comparison between Acupuncture and Nicotine Replacement Therapies for Smoking Cessation Based on Randomized Controlled Trials: A Systematic Review and Bayesian Network Meta-Analysis.

Evid Based Complement Alternat Med 2021 16;2021:9997516. Epub 2021 Jun 16.

School of Public Health, Center for Evidence-Based Medicine, Gansu University of Chinese Medicine, Lanzhou, Gansu 730000, China.

Objectives: To evaluate the efficacy and/or safety of acupuncture therapy (AT) in quitting smoking.

Methods: Randomized controlled trials (RCTs) were searched in PubMed, Cochrane Library, Embase, Web of Science, and Chinese Biomedical Database (CBM). We used Cochrane Collaborative Quality Assessment to assess the risk of bias. Bayesian network meta-analysis was utilized to evaluate the efficacy and safety of different interventions. Data analyses were conducted using WinBUGS 1.4.3, Stata 14, and RevMan 5.3.5 software.

Results: A total of 2706 patients from 23 studies were included, involving 6 treatment arms. Network meta-analysis demonstrated that there was no significant difference in short-term abstinence rates or changes in Fagerstrom test for nicotine dependence (FTND) scores and daily smoking among these groups (AT, sham acupuncture therapy (SAT), auricular acupressure (AA), sham auricular acupressure (SAA), acupuncture plus auricular acupressure (APAA), and nicotine replacement therapy (NRT)). However, there was a significant difference between SAA and AA with risk ratio (RR) of 2.49 (95% CI 1.14, 5.97) in long-term abstinence rate. The probabilistic ranking results showed that APAA and AA were superior to other interventions in the comparison of abstinence rates. There was no obvious inconsistency between the direct comparison and indirect comparison, using the consistency test.

Conclusion: AA was superior to SAA in smoke quitting, but there was no difference among other interventions in long-term truncation rates. There was no difference in short-term abstinence rates among these selected groups. We need large sample RCTs to clarify the advantages of interventions such as APAA and AA. In addition, reporting of adverse events that may occur during treatment also should be enhanced to complement evidence-based medicine. The trial is registered with PROSPERO CRD42020164712.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/9997516DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225439PMC
June 2021

m A modification of lncRNA PCAT6 promotes bone metastasis in prostate cancer through IGF2BP2-mediated IGF1R mRNA stabilization.

Clin Transl Med 2021 06;11(6):e426

Department of Orthopaedic Surgery, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.

Background: Bone metastasis is the leading cause of tumor-related death in prostate cancer (PCa) patients. Long noncoding RNAs (lncRNAs) have been well documented to be involved in the progression of multiple cancers. Nevertheless, the role of lncRNAs in PCa bone metastasis remains largely unclear.

Methods: The expression of prostate cancer-associated transcripts was analyzed in published datasets and further verified in clinical samples and cell lines by RT-qPCR and in situ hybridization assays. Colony formation assay, MTT assay, cell cycle analysis, EdU assay, Transwell migration and invasion assays, wound healing assay, and in vivo experiments were carried out to investigate the function of prostate cancer-associated transcript 6 (PCAT6) in bone metastasis and tumor growth of PCa. Bioinformatic analysis, RNA pull-down, and RIP assays were conducted to identify the proteins binding to PCAT6 and the potential targets of PCAT6. The therapeutic potential of targeting PCAT6 by antisense oligonucleotides (ASO) was further explored in vivo.

Results: PCAT6 was upregulated in PCa tissues with bone metastasis and increased PCAT6 expression predicted poor prognosis in PCa patients. Functional experiments found that PCAT6 knockdown significantly inhibited PCa cell invasion, migration, and proliferation in vitro, as well as bone metastasis and tumor growth in vivo. Mechanistically, METTL3-mediated m A modification contributed to PCAT6 upregulation in an IGF2BP2-dependent manner. Furthermore, PCAT6 upregulated IGF1R expression by enhancing IGF1R mRNA stability through the PCAT6/IGF2BP2/IGF1R RNA-protein three-dimensional complex. Importantly, PCAT6 inhibition by ASO in vivo showed therapeutic potential against bone metastasis in PCa. Finally, the clinical correlation of METTL3, IGF2BP2, IGF1R, and PCAT6 was further demonstrated in PCa tissues and cells.

Conclusions: Our study uncovers a novel molecular mechanism by which the m A-induced PCAT6/IGF2BP2/IGF1R axis promotes PCa bone metastasis and tumor growth, suggesting that PCAT6 may serve as a promising prognostic marker and therapeutic target against bone-metastatic PCa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ctm2.426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181202PMC
June 2021

Treatment of posterolateral tibial plateau fractures with a rotational support plate and special pressurizer: technical note and retrospective case series.

J Orthop Surg Res 2021 Jun 23;16(1):407. Epub 2021 Jun 23.

Orthopaedic Trauma Service Center, Major Laboratory of Orthopaedic Biomechanics in Hebei Province, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China.

Background: In tibial plateau fractures, the posterolateral segment of the tibia plateau is frequently affected and challenging to treat. Although there are many surgical approaches and fixation methods for the treatment of these fractures, all of these methods have limitations. We designed a new rotational support plate (RSP) and a special pressurizer that can fix the fracture directly via the anterolateral approach. This method is advantageous because it leads to little trauma, involves a simple operation, and has a reliable fixation effect. This study details the technique of treating these fractures with the RSP and special pressurizer and provides the outcomes.

Methods: From May 2016 to January 2019, the data of 12 patients with posterolateral tibial plateau fractures treated with the RSP and special pressurizer in our hospital were retrospectively analyzed. Postoperative rehabilitation was advised, knee X-rays were taken at follow-ups, and fracture healing, complications, and knee range of motion were assessed. The Hospital for Special Surgery (HSS) knee score and Knee Injury and Osteoarthritis Outcome Score (KOOS) were used to evaluate knee function at the last follow-up.

Results: The average follow-up time of all patients was 16.5 months (range, 12-25 months). The average bony union time was 3.2 months (range, 3-4.5 months). At the last follow-up, the average knee range of motion was 138° (range, 107-145°). The average HSS score was 91 (range, 64-98). The average KOOS Symptoms score was 90 (range, 75-96). The average KOOS Pain score was 91 (range, 72-97). The average KOOS ADL score was 91 (range, 74-97). The average KOOS sport/recreation score was 83 (range, 70-90). The average KOOS QOL score was 88 (range, 69-93). Skin necrosis, incision infections, and fixation failure did not occur during the follow-up period.

Conclusions: With our newly designed RSP and special pressurizer, posterolateral tibial plateau fractures can be easily and effectively reduced and fixed through the anterolateral approach, which serves as a novel treatment for posterolateral tibial plateau fractures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-021-02544-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220735PMC
June 2021

Florivory of Early Cretaceous flowers by functionally diverse insects: implications for early angiosperm pollination.

Proc Biol Sci 2021 06 16;288(1953):20210320. Epub 2021 Jun 16.

College of Life Science and Academy for Multidisciplinary Studies, Capital Normal University, Beijing, People's Republic of China.

Florivory (flower consumption) occurs worldwide in modern angiosperms, associated with pollen and nectar consumption. However, florivory remains unrecorded from fossil flowers since their Early Cretaceous appearance. We test hypotheses that earliest angiosperms were pollinated by a diverse insect fauna by evaluating 7858 plants from eight localities of the latest Albian Dakota Formation from midcontinental North America, in which 645 specimens (8.2%) were flowers or inflorescences. Well-preserved specimens were categorized into 32 morphotypes, nine of which displayed 207 instances of damage from 11 insect damage types (DTs) by four functional-feeding groups of hole feeding, margin feeding, surface feeding and piercing-and-sucking. We assessed the same DTs inflicted by known florivores on modern flowers that also are their pollinators, and associated insect mouthpart types causing such damage. The diverse, Dakota florivore-pollinator community showed a local pattern at Braun's Ranch of flower morphotypes 4 and 5 having piercing-and-sucking as dominant and margin feeding as minor interactions, whereas at Rose Creek I and II had an opposite pattern. We found no evidence for nectar robbing. These data support the rapid emergence of early angiosperms of florivore and associated pollinator guilds expressed at both the local and regional community levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1098/rspb.2021.0320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207559PMC
June 2021

An investigation of the effectiveness of vibration-reducing gloves for controlling vibration exposures during grinding handheld workpieces.

Appl Ergon 2021 Sep 12;95:103454. Epub 2021 May 12.

Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, 26505, USA.

Prolonged and intensive vibration exposures during the grinding of handheld workpieces may cause hand-arm vibration syndrome. The objectives of this study are to develop an on-the-hand method for evaluating vibration-reducing (VR) gloves, and to determine whether VR gloves can significantly reduce the vibration exposures. A worker holding and pressing a typical workpiece (golf club head) against a grinding wheel or belt in order to shape the workpiece was simulated, and the input vibration and those on the workpiece and hand-arm system were measured. Ten human subjects participated in the experiment. The results demonstrate that VR gloves significantly reduced the vibrations at the palm, hand dorsum, and wrist. The grinding interface condition and hand feed force did not substantially affect glove effectiveness. The use of gloves slightly increased the workpiece resonant response, but the resonant response did not significantly affect glove effectiveness. This study concluded that the use of VR gloves can help control vibration exposures of workers performing grinding of handheld workpieces.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.apergo.2021.103454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262383PMC
September 2021

New species and material of Hagloidea (Insecta, Ensifera) from the Yanliao biota of China.

Zookeys 2021 22;1033:183-190. Epub 2021 Apr 22.

College of Life Sciences, Capital Normal University, 105 Xisanhuanbeilu, Haidian District, Beijing, 100048, China Capital Normal University Beijing China.

A new species of Cyrtophyllitinae, Gu, Yue & Ren, is described from the Middle Jurassic Jiulongshan Formation, Daohugou Village, Inner Mongolia, China. The species is characterized by its ScA reaching the anterior wing margin at the level of the divergence of M+ CuA, distally branched RP, lengths of free CuA and free M equal, and numerous branches of CuA + CuPaα. A new fossil of Gu & Ren, 2012 is described which increases knowledge of its wing venation and indicates that should be assigned to the Tuphellidae.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3897/zookeys.1033.63571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084857PMC
April 2021

Effects of endplate healing morphology on intervertebral disc degeneration after pedicle screw fixation for thoracolumbar fractures.

Medicine (Baltimore) 2021 Apr;100(17):e25636

Orthopaedic Trauma Service Center, The Third Hospital of Hebei Medical University, Major Laboratory of Orthopaedic Biomechanics in Hebei Province.

Abstract: The cartilage endplate plays an important role in the stress distribution and nutrition metabolism of the intervertebral disc. The healing morphology of the endplate after spinal fracture and its effect on the intervertebral disc degeneration are still unclear.This was a retrospective study. Patients with traumatic single-level thoracolumbar fractures treated in our orthopedic trauma service center from June 2011 to May 2019 were included and the relevant data were collected from the medical records. Based on combined computed tomography and MRI images, the endplate injury status was determined (no endplate injury, unilateral and bilateral endplate injury). According to the location of the injury, endplate injury was further divided into endplate central injury and endplate peripheral injury. The degree of posttraumatic disc lesions and disc degeneration during follow-up were classified based on the Sander classification and the Pfirrmann classification, respectively. According to the T1 image of MRI at the final follow-up, the healing morphology of endplates was determined and classified. Univariate analyses and correlation analyses were performed to evaluate the within- and between-group differences.There were in total 51 patients included in this study. Cartilage endplate fracture was significantly closely related to the degree of degeneration of the intervertebral disc (P = .003). Injuries in different parts of the endplate have no significant effect on the intervertebral disc degeneration (P = .204). The healing morphology after endplate fracture significantly affected the degree of intervertebral disc degeneration (P = .001). The comparisons of groups showed that the effects of irregular healing and traumatic Schmorl nodes on disc degeneration were not statistically significant, but were significantly significant with increased curvature.These results suggest that the irregular healing and the traumatic Schmorl nodes are closely related to intervertebral disc degeneration. The presence and severity of the endplate injury can provide valuable information for individualized clinical decision-making processes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084067PMC
April 2021

Jurassic scorpionflies (Mecoptera) with swollen first metatarsal segments suggesting sexual dimorphism.

BMC Ecol Evol 2021 03 20;21(1):47. Epub 2021 Mar 20.

College of Life Sciences and Academy for Multidisciplinary Studies, Capital Normal University, 105 Xisanhuanbeilu, Haidian District, Beijing, 100048, China.

Background: Sexual dimorphism is widespread in insects. The certain specialized structures may be used as weapons in male-male combats or as ornaments to enhance mating opportunities.

Results: We report striking swollen first tarsal segments in two families, four genera and six species of scorpionflies from the Middle Jurassic Yanliao Biota of Northeastern China. Swollen tarsal segments are restricted to male specimens and to hind leg tarsi. The geometric morphometric analyses reveal that the degree of swelling within the orthophlebiid species possessing swollen first metatarsal segments is species-specific, which can be used as a diagnostic character for taxonomic and phylogenetic studies.

Conclusions: The new findings indicate that swollen first metatarsal segments are relatively common in the family Orthophlebiidae during the Middle Jurassic. The tarsal swellings are considered to be sexually dimorphic, potentially associated with sexually display by males and/or camouflage of a "nuptial gift" in the mating process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12862-021-01771-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981964PMC
March 2021

Temporal and Spatial Evolution of China's Human Development Index and Its Determinants: An Extended Study Based on Five New Development Concepts.

Soc Indic Res 2021 Mar 6:1-36. Epub 2021 Mar 6.

School of Statistics, Southwestern University of Finance and Economics, No.555, Liutai Road, Wenjiang District, Chengdu, 611130 China.

This paper innovatively expands China's human development index under the background of development concept in the new era, constructs the index system of China's human development index (CHDI) with four core dimensions of "life span, education, income and sustainable development" and measures the human development level of 31 provinces and the whole country from 1990 to 2017. Using the method of exploratory spatial data analysis (ESDA), this paper studies the spatiotemporal evolution characteristics of CHDI in 31 provinces from 1990 to 2017 and discusses the mechanism of CHDI in China. The results show that: ① CHDI in eastern China is obviously ahead of other regions and there is a big gap in human development among different regions. CHDI of 31 provinces had positive spatial correlation, showing significant spatial aggregation effect. ② In addition to its own influencing factors, the CHDI of a province is also affected by the CHDI of its "neighboring" provinces. ③ Urbanization rate, per capita financial expenditure on education and the number of health professionals are the three main positive driving factors of China's CHDI and the per capita carbon emission is the main reverse driving factor. This study provides policy suggestions for improving the level of human development in China and realizing regional balanced development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11205-021-02639-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936590PMC
March 2021

The occurrence rate and clinical application of Osteo-line on the femur neck.

J Orthop Surg Res 2021 Feb 25;16(1):158. Epub 2021 Feb 25.

Department of Orthopedic Surgery, The 3rd Hospital of Hebei Medical University, Shijiazhuang, People's Republic of China.

Background: This study was done to observe the incidence of Osteo-line on the femur neck and to explore the clinical application of Osteo-line in osteotomy.

Methods: Eighty-nine adult femur specimens were selected to observe the incidence of Osteo-line on the femur neck. From August 2015 to January 2019, a total of 278 patients who completed unilateral hip arthroplasty at the Third Hospital of Hebei Medical University were retrospectively included. Patients who accepted osteotomy via Osteo-line on the femur neck were defined as the experimental group (n = 139), and patients who accepted osteotomy via traditional method (The femoral distance 1.5 cm above the trochanter was retained for osteotomy by visual inspection.) were defined as the control group (n = 139). According to the postoperative pelvic X-ray, Photoshop was used to evaluate the leg length discrepancy (LLD) by the CFR-T-LT method.

Results: Among the 89 specimens, the incidence of anterior Osteo-line was 75.28%, and the incidence of posterior Osteo-line was 100%. According to the clinical application results, the incidence of anterior Osteo-line on the femur neck was 80%, and the incidence of posterior Osteo-line was 100%. The Osteo-line was clearer than those on the femoral specimens. Twenty-six cases had LLD greater than 1 cm (9.29%), including 2 cases in the experimental group and 24 cases in the control group. The average postoperative LLD in the experimental group (0.19 ± 0.38 mm) was significantly shorter than in the control group (0.54 ± 0.51 mm)(P = 0.005).

Conclusion: The incidence of Osteo-line on the femur neck was high, and patients who accepted osteotomy via Osteo-line on the femur neck can achieve shorter postoperative LLD than the control group.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-021-02289-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7905579PMC
February 2021

Subfamily Anischiinae (Coleoptera: Eucnemidae) in Early Cretaceous of Northeast China.

Insects 2021 Jan 26;12(2). Epub 2021 Jan 26.

College of Life Sciences and Academy for Multidisciplinary Studies, Capital Normal University, Xisanhuanbeilu 105, Haidian District, Beijing 100048, China.

new genus, type species new species (Eucnemidae, Anischiinae) is described from the Lower Cretaceous of Liaoning, China. The presence of this species in early Cretaceous deposits provides new insight into the evolution of basal lignicolous Eucnemidae clades. Both Anischiinae and Palaeoxeninae species diversified in a world dominated by gymnosperms, before the main radiation of angiosperms. More than 95% of modern eucnemid larvae have a -type highly modified head structure, but contrary to the larva, they develop in angiosperm wood. Anischiinae utilize angiosperms as well, but their head capsule shows no such modifications. These facts prove that highly specialized morphological features do not offer definite proof of similar way of life in the distant past, nor should non-modified structures be taken as proof for another kind of substrate choice. Eucnemidae have invaded angiosperms with two quite different morphological adaptations. This fact may have implications for the evolution of all clicking elateroids.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/insects12020105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910996PMC
January 2021

Functional Analysis of Estrogen Receptor 1 in Diabetic Wound Healing: A Knockdown Cell-Based and Bioinformatic Study.

Med Sci Monit 2020 Dec 18;26:e928788. Epub 2020 Dec 18.

Department of Hand Surgery, Wuhan Fourth Hospital; Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China (mainland).

BACKGROUND Diabetic wound (DW) treatment is a serious challenge for clinicians, and the underlying mechanisms of DWs remain elusive. We sought to identify the critical genes in the development of DWs and provide potential targets for DW therapies. MATERIAL AND METHODS Datasets of GSE38396 from the Gene Expression Omnibus (GEO) database were reviewed. Pathway analysis was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology term analyses were carried out, and Cytoscape software (Cytoscape 3.7.2) was used to construct the protein interaction network. Serum samples from patients with diabetes and control participants were collected, and the expression of estrogen receptor 1 (ESR1) was measured by quantitative reverse-transcription polymerase chain reaction. In addition, the function of ESR1 in human skin fibroblasts was investigated in vitro. RESULTS Eight samples were analyzed using the Morpheus online tool, which identified 637 upregulated and 448 downregulated differentially expressed genes. The top 5 KEGG pathways of upregulated differentially expressed genes were associated with sphingolipid metabolism, estrogen signaling, ECM-receptor interaction, MAPK signaling, and PI3K-Akt signaling. The hub genes for DWs were JUN, ESR1, CD44, SMARCA4, MMP2, BMP4, GSK3B, WDR5, PTK2, and PTGS2. JUN, MMP2, and ESR1 were the upregulated hub genes, and ESR1 was found to be consistently enriched in DW patients. Inhibition of ESR1 had a stimulative role in human skin fibroblasts. CONCLUSIONS ESR1 was identified as a crucial gene in the development of DWs, which suggests potential therapeutic targets for DW healing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12659/MSM.928788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7754692PMC
December 2020

The Clicking Elateroidea from Chinese Mesozoic Deposits (Insecta, Coleoptera).

Insects 2020 Dec 9;11(12). Epub 2020 Dec 9.

College of Life Sciences and Academy for Multidisciplinary Studies, Capital Normal University, Xisanhuanbeilu 105, Haidian District, Beijing 100048, China.

Recent molecular studies have suggested that the clicking beetle families Elateridae, Eucnemidae, Throscidae, and Cerophytidae evolved in the Jurassic and diversified in the Cretaceous. These studies paid little attention to fossils, using them only as dating tools. The identification of Elateridae fossils is challenging, as external synapomorphies are not known for this family. Elateridae can be identified only as something not belonging to the other related families, all of which have diagnostic synapomorphies. Most subfamilies and tribes of Elateridae do possess definite diagnostic characters, however, making their identification feasible. We checked the 28 Elateridae described from Chinese Mesozoic deposits. Twelve were Elateridae, seven were Eucnemidae, and one was a Throscidae. Three species could be Eucnemidae, but showed aberrant characters. Five species could not be placed and may not belong to Elateroidea at all. On the basis of these results we suggest that all previously described Elateridae fossils should be re-checked. They should be searched for synapomorphies defining Eucnemidae, Throscidae, and Cerophytidae. If such characters are not present, a click beetle type of fossil can be placed in Elateroidae . The Mesozoic Chinese Elateridae fossils all belong to clades that do not exist today, whereas the Mesozoic Eucnemidae subfamilies are extant ones. This may be the source of the disagreement between Elateridae fossil age and datings based on molecular studies. One new combination was made: (Chang, Kiretjshuk & Ren, 2009) NEW COMBINATION (= Chang, Kirejtshuk & Ren, 2009).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/insects11120875DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764174PMC
December 2020

New lance lacewings (Osmylidae: Kempyninae) from the Middle Jurassic of Inner Mongolia, China.

Zootaxa 2020 Aug 4;4822(1):zootaxa.4822.1.4. Epub 2020 Aug 4.

College of Life Sciences and Academy for multidisciplinary Studies, Capital Normal University, 105 Xisanhuanbeilu, Haidian District, Beijing 100048, China.

Contrary to the typical southern distribution of the extant Kempyninae, a subfamily of Osmylidae, its fossil group shows the high diversification in the northern hemisphere during the Mesozoic. Herein a new genus with a new species, Mirokempynus profundobifurcus gen. et sp. nov., and a new species, Jurakempynus loculosus sp. nov., of Kempyninae, are described from the Middle Jurassic Jiulongshan Formation of Daohugou Village, Inner Mongolia, China. The new species share the characteristic synapomorphies of Kempyninae, e.g. subcostal veinlets mostly irregularly forked in the forewing and the distinctly expanded intramedial area with multiple rows of cells in the hind wing. The new genus distinctly shows a particular condition of intramedial area and MP branching in the hind wing. However, the condition of the region between MA and MP significantly broadened in the hind wing appear distinctively different to other known kempynine genera. A key is provided for all the genera of documented kempynines, both fossil and extant.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11646/zootaxa.4822.1.4DOI Listing
August 2020

A new macrolepidopteran moth (Insecta, Lepidoptera, Geometridae) in Miocene Dominican amber.

Zookeys 2020 3;965:73-84. Epub 2020 Sep 3.

College of Life Sciences and Academy for Multidisciplinary Studies, Capital Normal University, 105 Xisanhuanbeilu, Haidian District, Beijing 100048, China Capital Normal University Beijing China.

A new genus and species of fossil moth, Zhang, Shih & Shih, , assigned to Geometridae, is described from Miocene Dominican amber dating from 15-20 Mya. The new genus is characterized by the forewing without a fovea, R not anastomosing with Sc, no areole formed by veins R and Rs, R and Rs completely coincident, M arising midway between M and M, anal veins 1A and 2A fused for their entire lengths; and the hind wing with Rs running close to Sc + R and M absent.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3897/zookeys.965.54461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483395PMC
September 2020

Behaviors and Interactions of Insects in Mid-Mesozoic Ecosystems of Northeastern China.

Annu Rev Entomol 2021 01 11;66:337-354. Epub 2020 Sep 11.

College of Life Sciences and Academy for Multidisciplinary Studies, Capital Normal University, Beijing 100048, China; email:

During the past 20 years, more than 1,600 species of well-preserved fossil insects, including members of over 270 families within 24 orders, have been described from the Middle Jurassic Yanliao Entomofauna and Early Cretaceous Jehol Entomofauna in Northeastern China. Diversified fossil insects not only document the origin, systematics, and early evolution of many lineages, but also reveal these lineages' behaviors and interactions with coexisting plants, vertebrates, and other insects in their ecosystems. Fossil evidence has been documented, for example, regarding insects' feeding and pollination mutualism with gymnosperms; ectoparasitic feeding on blood of vertebrates; camouflage, mimicry of gymnosperm plants, and eyespot warning; sound stridulation for attracting potential mates; and sexual display, mating, egg-laying, and parental care. In this article, we review the diverse taxonomy of mid-Mesozoic insects of Northeastern China and elucidate their behaviors and interactions within their ecosystems, which have impacted their early evolution and development into extant insects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1146/annurev-ento-072720-095043DOI Listing
January 2021

MYC-associated protein X binding with the variant rs72780850 in RNA helicase DEAD box 1 for susceptibility to neuroblastoma.

Sci China Life Sci 2021 Jun 8;64(6):991-999. Epub 2020 Sep 8.

Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, MOE Key Laboratory of Major Diseases in Children, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China.

Neuroblastoma (NB) is one of the most common malignant tumors in children, with variable clinical behaviors and a 15% death rate of all malignancies in childhood. However, genetic susceptibility to sporadic NB in Han Chinese patients is largely unknown. To identify genetic risk factors for NB, we performed an association study on 357 NB patients and 738 control subjects among Han Chinese children. We focused on DEAD box 1 (DDX1), a putative RNA helicase, which is involved in NB carcinogenesis. The potential association of DDX1 polymorphisms with NB has not been discovered. Our results demonstrate that rs72780850 (NM_004939.2:c.-1555T>C) located in the DDX1 promoter region is significantly associated with higher expression of DDX1 transcript and increased NB risk (odds ratio=1.64, 95% confidence interval=1.03%-2.60%, P=0.004), especially in aggressive NB compared with ganglioneuroma and ganglioneuroblastoma in a dominant model (TC+CC vs. TT). Furthermore, the MYC-associated protein X (MAX) transcription factor showed stronger binding affinity to the DDX1 rs 72780850 CC allele compared with the TT allele, explaining the molecular mechanism of the increased NB risk caused by the rs72780850 polymorphism. Our results highlight the involvement of regulatory genetic variants of DDX1 in NB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11427-020-1784-7DOI Listing
June 2021

Effect of straw-derived dissolved organic matter on the adsorption of sulfamethoxazole to purple paddy soils.

Ecotoxicol Environ Saf 2020 Oct 21;203:110990. Epub 2020 Jul 21.

College of Environmental Science and Engineering, China West Normal University, Sichuan, 637009, China; Key Laboratory of Solid Waste Treatment and Resource Recycle, Ministry of Education, Southwest University of Science and Technology, Sichuan, 621010, China. Electronic address:

The presence of sulfamethoxazole (SMX) in croplands has become an international concern. The environmental behavior and fate of SMX in agricultural soils are not well understood, especially when the adsorption behavior is disturbed by the dissolved organic matter (DOM) released by crop straw. As canola straw is one of the biomasses widely returned to farmlands, we characterized DOM derived from pristine and decomposed canola straw, and explored the effects and mechanisms of the DOMs on regulating SMX adsorption to purple paddy soils. The spectral analysis showed that the molecular weight, aromaticity, and hydrophobicity of canola straw-derived DOM increased as decomposition proceeded. These physicochemical properties collectively determined the effects of the DOM on SMX adsorption. The DOM derived from pristine canola straw increased SMX maximum adsorption capacity of the soils by approximately 2.6 times, but this positive effect gradually decreased to a steady state by day 90 in the straw decomposition period. Nevertheless, the SMX adsorption behavior in the soils was invariably determined by the DOM extracts. These adsorption processes of SMX were well fitted by the double-chamber kinetics model and the Langmuir and Freundlich thermodynamic models. Thermodynamic parameters indicated that SMX adsorption onto the soils was spontaneous and endothermic, and this adsorption characteristics was not significantly (p > 0.05) changed by the DOM extracts. However, the adsorption kinetics were altered by those DOMs, i.e., the fast and slow adsorption processes were both diminished. Correspondingly, co-adsorption and cumulative adsorption were identified as the main mechanisms determining SMX adsorption to the purple paddy soils in the presence of the straw-derived DOMs. These results collectively indicated that the DOMs released by straw in croplands may decrease the ecological risks of organic pollutants by inhibiting their migration processes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2020.110990DOI Listing
October 2020

Upregulation of Sirt1 by tyrosol suppresses apoptosis and inflammation and modulates extracellular matrix remodeling in interleukin-1β-stimulated human nucleus pulposus cells through activation of PI3K/Akt pathway.

Int Immunopharmacol 2020 Nov 28;88:106904. Epub 2020 Aug 28.

Department of Spine Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, China.

Intervertebral disc degeneration (IDD) is the major pathogenesis of lower back pain. Tyrosol is a polyphenolic compound that exhibits anti-oxidant, anti-apoptotic, and anti-inflammatory effects. Herein, we explored the effects and mechanisms of tyrosol on IDD progression in interleukin (IL)-1β-stimulated human nucleus pulposus cells (HNPCs). Cell viability and apoptosis were detected by CCK-8 and flow cytometry analysis, respectively. The production of tumor necrosis factor-α (TNF-α), IL-6, nitric oxide (NO), and prostaglandin E2 (PGE2) was examined to evaluate inflammation. The mRNA expression of matrix metalloproteinases (MMPs) (MMP-3/9/13), collagen type II, SRY-related high mobility group box 9 (SOX-9), and aggrecan was measured by qRT-PCR. Protein levels of silent information regulator 2 homolog 1 (Sirt1), phosphorylated protein kinase B (p-Akt), Akt, collagen type II, SOX-9, and aggrecan were determined by western blot. Results showed that tyrosol attenuated IL-1β-induced viability reduction, apoptosis, and caspase-3/7 activity in HNPCs. The increase in the production of TNF-α, IL-6, NO, and PGE2 in IL-1β-treated HNPCs was abolished by tyrosol treatment. Tyrosol treatment reversed IL-1β-induced upregulation of MMP-3, MMP-9, and MMP-13, and downregulation of collagen II, SOX-9, and aggrecan in HNPCs. Additionally, tyrosol treatment activated the phosphatidylinositol 3-kinase (PI3K)/Akt pathway in IL-1β-stimulated HNPCs. Sirt1 was upregulated by tyrosol, and Sirt1 silencing inhibited Akt phosphorylation in HNPCs. Sirt1 knockdown attenuated the effects of tyrosol on IL-1β-induced apoptosis, inflammation, and ECM remodeling in HNPCs. In summary, upregulation of Sirt1 by tyrosol suppressed apoptosis and inflammation and regulated ECM remodeling in IL-1β-stimulated HNPCs through activation of PI3K/Akt pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2020.106904DOI Listing
November 2020

A world key to the genera of Elcanidae (Insecta, Orthoptera), with a Jurassic new genus and species of Archelcaninae from China.

Zookeys 2020 29;954:65-74. Epub 2020 Jul 29.

College of Agronomy, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China Sichuan Agricultural University, Chengdu China.

A new fossil genus and species is described from the Middle Jurassic of China. The type of has body and legs preserved. It is distinguished from all other elcanids by the unique combination of wing venation and stout ovipositor. The sickle-shaped ovipositor suggests that the new species had a preference for oviposition on plant material. A world key to the genera of Elcanidae is provided based on the wing venation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3897/zookeys.954.52088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406534PMC
July 2020

Multiple roles of dissolved organic matter released from decomposing rice straw at different times in organic pollutant photodegradation.

J Hazard Mater 2021 01 8;401:123434. Epub 2020 Jul 8.

State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China. Electronic address:

Rice straw returning causes a considerable amount of dissolved organic matter (DOM) release into aquatic croplands in a relatively short-term. The presence of rice straw-derived DOM in cropland waters may alter the photochemical behaviors of organic pollutants. However, the photochemical activity and photosensitization role of the DOMs are poorly understood. Here, eight DOM samples were extracted from decomposing rice straw at different times in 49 days to explore their photosensitizing capacities toward diuron (DIU), 17β-estradiol (E2), and sulfamethoxazole (SMX). All of the DOMs were photosensitive and mainly composed of tryptophan-, tyrosine- and fulvic-like substances. Over the decomposition period, the amount of photochemically produced reactive intermediates (PPRIs) by the DOMs peaked on days 7 and 14. The evolution of the DOM photosensitizing capacity towards DIU and E2 was consistent with the variations of PPRIs, and HO· was confirmed as a critical factor. However, the influence of the DOMs on SMX photodegradation was opposite to that on DIU and E2. The positive role of the DOMs in SMX photodegradation was attributed to the tryptophan-like components. The results suggest that straw-derived DOM is an important photosensitizer and that its photosensitization towards organic pollutants is dependent on straw decomposing time and pollutant type.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2020.123434DOI Listing
January 2021

Lichen mimesis in mid-Mesozoic lacewings.

Elife 2020 07 29;9. Epub 2020 Jul 29.

College of Life Sciences and Academy for Multidisciplinary Studies, Capital Normal University, Beijing, China.

Animals mimicking other organisms or using camouflage to deceive predators are vital survival strategies. Modern and fossil insects can simulate diverse objects. Lichens are an ancient symbiosis between a fungus and an alga or a cyanobacterium that sometimes have a plant-like appearance and occasionally are mimicked by modern animals. Nevertheless, lichen models are almost absent in fossil record of mimicry. Here, we provide the earliest fossil evidence of a mimetic relationship between the moth lacewing mimic gen. nov. and its co-occurring fossil lichen model . We corroborate the lichen affinity of and document this mimetic relationship by providing structural similarities and detailed measurements of the mimic's wing and correspondingly the model's thallus. Our discovery of lichen mimesis predates modern lichen-insect associations by 165 million years, indicating that during the mid-Mesozoic, the lichen-insect mimesis system was well established and provided lacewings with highly honed survival strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7554/eLife.59007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7462608PMC
July 2020

Treatment of the tibial pilon fractures using the antero-medial fibula approach: Ten case series.

Medicine (Baltimore) 2020 Jul;99(28):e20576

Orthopedic Trauma Service Center, Third Hospital of Hebei Medical University, Major Laboratory of Orthopedic Biomechanics in Hebei Province, Shijiazhuang, Hebei Province, China.

Pilon fractures are always results of the high-energy trauma. They are often accompanied with serious soft tissue injury, and tension blisters happened in most cases. For comminuted fractures and poor soft tissue, how to select the incision is challenging. This study aimed to explore the outcomes of the treatment of ten 43-B/C pilon fractures using an anteromedial fibula approach.Ten closed pilon fractures combined with fibula fractures were treated in our hospital from January 2015 to July 2016. Six cases were AO/OTA type 43-B and 4 cases were 43-C, including 9 males and 1 female with a mean age of 36.3 years (range: 20-60 years). When the skin wrinkled, all patients were treated by the senior authors with open reduction and internal fixation using an anteromedial fibula approach. Postoperatively, patients were followed up at 1 month, 3 months, 6 months, 12 months, and 18 months, respectively. The incision healing, the American Orthopedic Foot and Ankle Society scores and fracture healing were recorded to get a comprehensive evaluation of the effect for the incision.All patients were followed from 9 to 18 months (average: 14.1 months). Anatomic reduction was achieved in 7 cases and satisfactory in 3 cases by the Burwell-Charnley radiological criteria evaluation. All patients had complete retention of the dorsal extensor tendon sheath. The most incisions had a good healing without necrosis at 2 weeks after surgery except 1 case. The factures were healed at a range of 12 to 18 weeks (average: 13.7 ± 1.2 weeks). The American Orthopedic Foot and Ankle Society scores were excellent in 7 cases and good in 3 cases at 1 year after surgery (average: 85.6 ± 4.2 points). The satisfactory outcomes were achieved in most patients.The anteromedial fibula approach used for pilon fractures can lead to an effective exposure and allow fixation of tibia and fibula fractures with minimal soft tissue injury. It is a safe, simple, and effective approach that allows for satisfactory functional rehabilitation of the ankle joint. LEVEL OF EVIDENCE:: therapeutic Level IV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000020576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360227PMC
July 2020

Spatial distribution, risk assessment, and source identification of heavy metals in water from the Xiangxi River, Three Gorges Reservoir Region, China.

Environ Geochem Health 2021 Feb 13;43(2):915-930. Epub 2020 Jun 13.

College of Hydraulic and Environmental Engineering, China Three Gorges University, Yichang, 443002, Hubei, China.

Heavy metals (HMs) contamination in rivers has attracted wide concern due to its persistence and potential risks to the natural environment and human health. In this study, eight HMs (As, Hg, Cu, Pb, Ca, Zn, Mn, and Ni) were measured by inductively coupled plasma mass spectrometry in 24 water samples to investigate HMs contamination levels in the Xiangxi River of the Yangtze River basin. A geographic information systems kriging interpolation method was used to reveal the spatial distribution of HMs contamination. The results indicate that most HMs occurred at acceptable levels below the Surface Water Quality Standard (GB 3838-2002), with the highest concentration (23.23 mg kg) of Mn being observed at sampling site X20. The values of the potential ecological risk index (RI) suggest that high potential ecological risks were present at sampling sites X1, X3, X4, X14, X16, X17, and X24, which reached moderate risk level. The highest value of RI (279.56) was observed at site X17. HM spatial distributions show that upstream pollution is more severe than downstream. The hazard index was below 1 for all HMs except for Mn, indicating that HMs in Xiangxi River pose a low risk to human health. HM source identification was accomplished using principal component analysis and Pearson's correlation. Cu, Cd, Ni, and Hg originate primarily from agriculture, while Pb, Zn, and As originate primarily from transportation and mining. This research provides a reference on the risks posed by HMs in Xiangxi River and will support efforts to protect and improve water quality in Xiangxi River.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10653-020-00614-2DOI Listing
February 2021

Heavy metal accumulation and health risk assessment of crayfish collected from cultivated and uncultivated ponds in the Middle Reach of Yangtze River.

Sci Total Environ 2020 Oct 5;739:139963. Epub 2020 Jun 5.

College of Hydraulic and Environmental Engineering, China Three Gorges University, Yichang 443002, Hubei, China; Innovation Center for Geo-Hazards and Eco-Environment in Three Gorges Area, Hubei Province, Yichang 443002, China; Engineering Research Center of Eco-environment in Three Gorges Reservoir Region, Ministry of Education, China Three Gorges University, Yichang 443002, Hubei, China. Electronic address:

Crayfish, an aquatic food consumed globally, has been reported to accumulate heavy metal. To assess the health risk to the crayfish-consuming population, we investigated heavy metals (Cu, Pb, Zn, Fe, Mn, Cd, Cr, Ba, and As) in crayfish tissues (exoskeleton, abdominal muscle, gills, and hepatopancreas) and sediments from uncultivated and cultivated ponds in the middle reach of the Yangtze River. The metals, except for As, were measured by Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) and As was measured by atomic fluorescence spectrometry. The distribution of heavy metals differed significantly among tissues and concentrations were generally highest in the hepatopancreas. The average concentrations of heavy metals were higher in crayfish from uncultivated ponds than in crayfish from cultivated ponds. Heavy metal concentrations in crayfish correlated positively with sediment concentrations in uncultivated ponds, indicating that heavy metals can accumulate in crayfish. However, this positive correlation between sediment concentration and crayfish concentration was not observed in cultivated ponds. Source identification, using correlation analysis, indicated that heavy metals in the sediments derive from transportation and agriculture. Based on correlations between heavy metal concentration and body weight, and data on metal distribution in organs, heavy metals accumulate in the hepatopancreas of crayfish in uncultivated ponds, but not in cultivated ponds. Heavy metal concentrations were somewhat higher in female crayfish than in male crayfish and the difference was significant for Fe and Pb. The target hazard quotient (THQ) was used to evaluate non-carcinogenic health risk, with THQ < 1 considered to be safe. All THQ values were < 1, except for Cu in the hepatopancreas (THQ = 1.38) of cultured crayfish and Pb (THQ = 1.01) and Cd (THQ = 1.69) in the hepatopancreas of wild crayfish. Thus, the health risk posed by consuming crayfish, particularly cultured crayfish is low. Our research was designed to protect food safety and the results indicate that the health risk posed by heavy metals in crayfish is low.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.139963DOI Listing
October 2020
-->