Publications by authors named "Ravi Mani"

3 Publications

  • Page 1 of 1

Susceptibility of betanodavirus in a newly established vertebra-derived cell line from Mosquitofish (Gambusia affinis).

J Fish Dis 2020 Feb 16;43(2):263-273. Epub 2019 Dec 16.

Centre for Ocean Research, Col. Dr Jeppiaar Research Park, Sathyabama Institute of Science and Technology, Chennai, India.

In the present study, a new cell line from the vertebra of mosquitofish Gambusia affinis was successfully established and characterized. The cell line is named as bone Gambusia affinis (BGA) and subcultured for more than 55 passages in Leibovitz's/L15 medium supplemented with 15% FBS at 28°C. The cell line has a modal chromosome number of 48. Molecular characterization of the partial sequence of the coi gene confirmed the origin of the BGA cell line from mosquitofish. These cells exhibited epithelial morphology confirmed by the cytokeratin marker. The BGA cells showed mineralization of their extracellular matrix when stained with alizarin red and von Kossa stain. BGA cells were found to be susceptible to RGNNV and SJNNV strains of betanodavirus (NNV) showing cytopathic effect with multiple vacuolations in the cells. The RT-PCR confirmed the betanodavirus infections in BGA cells. The SEM micrograph showed the morphological changes observed in the cell during virus infection. The in vivo challenge experiment also showed the viral replicating efficiency in the Gambusia affinis with increasing viral titre. Thus, our present results show that the BGA cell line is a useful tool for isolating betanodavirus and could be used to investigate bone cell differentiation and extracellular matrix mineralization.
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http://dx.doi.org/10.1111/jfd.13127DOI Listing
February 2020

Maternal Obesity during Pregnancy Alters Daily Activity and Feeding Cycles, and Hypothalamic Clock Gene Expression in Adult Male Mouse Offspring.

Int J Mol Sci 2019 Oct 30;20(21). Epub 2019 Oct 30.

School of Human Development and Health, Faculty of Medicine, University of Southampton, Southampton General Hospital, Southampton SO16 6YD, UK.

An obesogenic diet adversely affects the endogenous mammalian circadian clock, altering daily activity and metabolism, and resulting in obesity. We investigated whether an obese pregnancy can alter the molecular clock in the offspring hypothalamus, resulting in changes to their activity and feeding rhythms. Female mice were fed a control (C, 7% kcal fat) or high fat diet (HF, 45% kcal fat) before mating and throughout pregnancy. Male offspring were fed the C or HF diet postweaning, resulting in four offspring groups: C/C, C/HF, HF/C, and HF/HF. Daily activity and food intake were monitored, and at 15 weeks of age were killed at six time-points over 24 h. The clock genes , , , and in the suprachiasmatic nucleus (SCN) and appetite genes and in the arcuate nucleus (ARC) were measured. Daily activity and feeding cycles in the HF/C, C/HF, and HF/HF offspring were altered, with increased feeding bouts and activity during the day and increased food intake but reduced activity at night. Gene expression patterns and levels of , , and in the SCN and and in the ARC were altered in HF diet-exposed offspring. The altered expression of hypothalamic molecular clock components and appetite genes, together with changes in activity and feeding rhythms, could be contributing to offspring obesity.
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http://dx.doi.org/10.3390/ijms20215408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6862679PMC
October 2019

Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon.

Fish Shellfish Immunol 2017 Jun 8;65:88-95. Epub 2017 Apr 8.

Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Road, Bangkok 10330, Thailand. Electronic address:

Spätzle is a signaling ligand in innate immune response that signals pathogenic infection via Toll receptor and Toll pathway into the cells for the synthesis of antimicrobial proteins. Herein, three PmSpӓtzle isoforms were identified in Penaeus monodon, namely PmSpz1, 2 and 3. The PmSpz1 was chosen for detailed study. The PmSpz1 gene was expressed in all nine tissues tested including the hemocytes, stomach, hepatopancreas, gill, lymphoid tissue, eyestalk, muscle, intestine and heart. Its expression was up-regulated upon white spot syndrome virus (WSSV) infection. Western blot analysis of hemolymph showed that the PmSpz1 mostly existed as a cleaved active form awaiting to activate the Toll pathway. Injection of a recombinant PmSpz1 rendered the shrimp less susceptible to the WSSV infection. Injection of a recombinant active form of PmSpz1 into a normal shrimp activated the synthesis of crustinPm1, crustinPm7, ALFPm3, penaeidin3 but not penaeidin5 indicating that the expression of all antimicrobial proteins but not penaeidin5 was under the regulation of Toll pathway.
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http://dx.doi.org/10.1016/j.fsi.2017.04.005DOI Listing
June 2017