Publications by authors named "Ran Yan"

147 Publications

Ligustrazine protects against chronic hypertensive glaucoma in rats by inhibiting autophagy via the PI3K-Akt/mTOR pathway.

Mol Vis 2021 12;27:725-733. Epub 2021 Dec 12.

Department of Ophthalmology, The Affiliated Traditional Chinese Medicine Hospital of Guangzhou Medical University, Guangzhou, China.

Purpose: Glaucoma is a leading cause of global irreversible blindness, and characterized by the progressive loss of retinal ganglion cells (RGCs). Ligustrazine (TMP) is a natural product that has shown beneficial effects on various diseases. This study aimed to determine whether ligustrazine produces a therapeutic effect on glaucoma and to investigate its underlying mechanisms.

Methods: A rat chronic hypertensive glaucoma model was induced by episcleral vein cauterization (EVC). Adult Sprague-Dawley (SD) rats were intraperitoneally administered TMP at a dose of 80 mg/kg once a day, from two days before EVC to one month after EVC. To elucidate the role of the mammalian target of rapamycin (mTOR) and phosphoinositide 3-kinase (PI3K), TMP-treated experimental rats were co-treated with the mTOR inhibitor rapamycin (5 mg/kg) or the PI3K inhibitor Ly294002 (10 mg/kg). The intraocular pressure (IOP) of the experimental and control rats was measured every six days. Retinal cells were examined by hematoxylin-eosin and terminal deoxynucleotidyltransferase-mediated biotinylated UTP nick end labeling (TUNEL) staining, as well as transmission electron microscopy. Immunohistochemistry and western blot analysis were performed to measure proteins involved in apoptosis and autophagy.

Results: Ligustrazine protected retinal cells from death in experimental glaucoma rats, which was not due to the lowering of IOP, but could be attributable to direct suppression of retinal cell apoptosis. In glaucoma rats, autophagy was markedly activated in retina cells, as evidenced by increased numbers of autophagosomes and the expression of autophagy-related proteins (ATG5 and LC3-II/I). Notably, such alterations in glaucoma rats were almost completely reversed by ligustrazine. The suppressive effects of ligustrazine on apoptosis and autophagy of retina cells were markedly attenuated by the mTOR inhibitor rapamycin or the PI3K inhibitor Ly294002. Additionally, ligustrazine significantly increased the protein levels of phosphorylated PI3K (p-PI3K), protein kinase B (p-Akt), and mTOR (p-mTOR) in glaucoma rats, whereas such increases were attenuated by rapamycin or Ly294002.

Conclusions: These results demonstrate that ligustrazine is protective in experimental glaucoma by inhibiting autophagy via the activation of the PI3K-Akt/mTOR pathway, providing compelling evidence that ligustrazine is potentially therapeutic for patients with glaucoma.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8711580PMC
December 2021

Long-acting recombinant human interleukin-7, NT-I7, increases cytotoxic CD8 T cells and enhances survival in mouse glioma models.

Clin Cancer Res 2022 Jan 14. Epub 2022 Jan 14.

Radiation Oncology, Washington University in St. Louis School of Medicine.

Purpose: Patients with glioblastoma (GBM) are treated with radiation therapy (RT) and temozolomide (TMZ). These treatments may cause prolonged systemic lymphopenia, which itself is associated with poor outcomes. NT-I7 is a long-acting IL-7 that expands CD4 and CD8 T cell numbers in humans and mice. We tested whether NT-I7 prevents systemic lymphopenia and improves survival in mouse models of GBM.

Experimental Design: C57BL/6 mice bearing intracranial tumors (GL261 or CT2A) were treated with RT (1.8 Gy/day x 5 days), TMZ (33 mg/kg/day x 5 days), and/or NT-I7 (10 mg/kg on the final day of RT). We followed the mice for survival while serially analyzing levels of circulating T lymphocytes. We assessed regulatory T cells (Treg) and cytotoxic T lymphocytes in the tumor microenvironment, cervical lymph nodes, spleen, and thymus; and hematopoietic stem and progenitor cells (HSPCs) in the bone marrow.

Results: GBM tumor-bearing mice treated with RT+NT-I7 increased T lymphocytes in the lymph nodes, thymus, and spleen, enhanced IFNγ production, and decreased T cells in the tumor which was associated with a significant increase in survival. NT-I7 also enhanced central memory and effector memory CD8 T cells in lymphoid organs and tumor. Depleting CD8 T cells abrogated the effects of NT-I7. Furthermore, NT-I7 treatment decreased progenitor cells in the bone marrow.

Conclusion: In orthotopic glioma-bearing mice, NT-I7 mitigates radiation-related lymphopenia, increases cytotoxic CD8 T lymphocytes systemically and in the tumor, and improves survival. A phase I/II trial to evaluate NT-I7 in patients with high-grade gliomas is ongoing (NCT03687957).
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http://dx.doi.org/10.1158/1078-0432.CCR-21-0947DOI Listing
January 2022

Targeting Amyloids with [F]AV-45 for Medullary Thyroid Carcinoma Positron Emission Tomography/Computed Tomography Imaging: A Pilot Clinical Study.

Mol Pharm 2022 Jan 4. Epub 2022 Jan 4.

Department of Nuclear Medicine, First Affiliated Hospital of Dalian Medical University, Liaoning 116021, People's Republic of China.

Medullary thyroid carcinoma (MTC) is a malignant neuroendocrine tumor with a high recurrence rate. Amyloid plaques formed from the misfolding of calcitonin are the key characteristics of MTC. Herein, we conducted a first-in-human pilot clinical study by applying a β-amyloid-specific radiotracer, [F]AV-45, to positron emission tomography (PET)/computed tomography (CT) imaging of MTC. The presence of amyloid plaques in the tumor tissue sections from five MTC patients was confirmed by hematoxylin and eosin (H&E) and Congo Red staining. [F]AV-45 selectively accumulated in the amyloid plaques in the continued tumor tissue sections with similar distribution patterns to the H&E and Congo Red staining. In addition, the [F]AV-45 uptake can be largely blocked by its nonradioactive reference compound. The [F]AV-45 accumulation in the thyroid, neck lymph nodes, and muscles in healthy human subjects is close to the background indicated by PET/CT imaging. In the comparison PET/CT imaging study of a recurrent MTC patient, 2-deoxy-2-[F]fluoro-d-glucose ([F]FDG) showed an elevated uptake by multiple neck lymph nodes. In contrast, only one of these neck lymph nodes had increased [F]AV-45 uptake. Postoperative histopathological analysis confirmed the [F]AV-45 PET-positive lymph node as MTC with amyloid deposition, while other [F]FDG positive lymph nodes were free from MTC and amyloid plaques. Thus, [F]AV-45 showed the promise for the clinical PET/CT imaging of MTC.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00680DOI Listing
January 2022

Notoginsenoside R1 Protects Against High Glucose-Induced Cell Injury Through AMPK/Nrf2 and Downstream HO-1 Signaling.

Front Cell Dev Biol 2021 1;9:791643. Epub 2021 Dec 1.

Nanmingtang Clinic, Guizhou Provincial People's Hospital, Guiyang, China.

Notoginsenoside R1 (NGR1), the primary bioactive compound found in Panax notoginseng, is believed to have antihypertrophic and antiapoptotic properties, and has long been used to prevent and treat cardiovascular diseases. However, its potential role in prevention of diabetic cardiomyopathy remains unclear. The present study aimed to investigate the mechanism of NGR1 action in high glucose-induced cell injury. H9c2 cardiomyocytes were cultured in a high-glucose medium as an model, and apoptotic cells were visualized using TUNEL staining. Expression of Nrf2 and HO-1 was measured using Western blotting or reverse transcription-quantitative PCR (RT-qPCR). The Nrf2 small interfering (si) RNA was transfected into cardiomyocytes using Opti-MEM containing Lipofectamine RNAiMAX. NGR1 protected H9c2 cardiomyocytes from cell death, apoptosis and hypertrophy induced by high glucose concentration. Expression of auricular natriuretic peptide and brain natriuretic peptide was remarkably reduced in NGR1-treated H9C2 cells. Western blot analysis showed that high glucose concentration markedly inhibited AMPK, Nrf2 and HO-1, and this could be reversed by NGR1 treatment. However, the cardioprotective effect of NGR1 was attenuated by compound C, which reverses Nrf2 and HO-1 expression levels, suggesting that AMPK upregulates Nrf2 and HO-1 gene expression, protein synthesis and secretion. Transfection of H9C2 cells with Nrf2 siRNA markedly reduced the cardioprotective effect of NGR1 via reduced expression of HO-1. These results indicated that NGR1 attenuated high glucose-induced cell injury via AMPK/Nrf2 signaling and its downstream target, the HO-1 pathway. We conclude that the cardioprotective effects of NGR1 result from upregulation of AMPK/Nrf2 signaling and HO-1 expression in cardiomyocytes. Our findings suggest that NGR1 treatment might provide a novel therapy for diabetic cardiomyopathy.
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http://dx.doi.org/10.3389/fcell.2021.791643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672164PMC
December 2021

An Antioxidant Enzyme Therapeutic for Sepsis.

Front Bioeng Biotechnol 2021 23;9:800684. Epub 2021 Nov 23.

Department of Chemical and Biomolecular Engineering, The University of California, Los Angeles, CA, United States.

Sepsis is a systemic inflammatory response syndrome caused by infections that may lead to organ dysfunction with high mortality. With the rapid increase in the aging population and antimicrobial resistance, developing therapeutics for the treatment of sepsis has been an unmet medical need. Excessive production of reactive oxygen species (ROS) during inflammation is associated with the occurrence of sepsis. We report herein a treatment for sepsis based on PEGylated catalase, which can effectively break down hydrogen peroxide, a key component of ROS that is chemically stable and able to diffuse around the tissues and form downstream ROS. PEGylated catalase can effectively regulate the cytokine production by activated leukocytes, suppress the elevated level of AST, ALT, TNF-α, and IL-6 in mice with induced sepsis, and significantly improve the survival rate.
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http://dx.doi.org/10.3389/fbioe.2021.800684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8650590PMC
November 2021

VO/NaVOnanocomposites synthesized by molten salt method as a high-performances cathode material for aqueous zinc-ion batteries.

Nanotechnology 2021 Dec 22;33(11). Epub 2021 Dec 22.

National Center for International Research on Photoelectric and Energy Materials, Yunnan University, Kunming 650091, People's Republic of China.

Aqueous zinc-ion batteries (ZIBs) is a potential energy storage system due to its advantages of low cost, good safety, and high theoretical capacity (820 mAh g). However, the lack of cathode materials with long cycle stability severely restricts the development of ZIBs. In this paper, VO/ NaVOnanocomposites are synthesized by molten salt method in one step and used as cathode material for ZIBs, which have good electrochemical performances. The specific capacity of the materials remain 160 mAh gwhen the current density is 0.5 A gafter 1000 cycles, and the capacity retention rate is 102.03% when the current density is 5 A gfor 1000 cycles. This is mainly due to the large number of active sites generated by crystal defects and the synergistic interaction between the dual-phase materials, which reduces the stress of ions inserted/extracted during the Znstorage process and improves the electrochemical performance.
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http://dx.doi.org/10.1088/1361-6528/ac3fe1DOI Listing
December 2021

A Reactivity-Based F-Labeled Probe for PET Imaging of Oxidative Stress in Chemotherapy-Induced Cardiotoxicity.

Mol Pharm 2022 Jan 30;19(1):18-25. Epub 2021 Nov 30.

School of Biomedical Engineering & Imaging Sciences, King's College London, King's Health Partners, St Thomas' Hospital, London SE1 7EH, United Kingdom.

Oxidative stress underlies the pathology of many human diseases, including the doxorubicin-induced off-target cardiotoxicity in cancer chemotherapies. Since current diagnostic procedures are only capable of monitoring cardiac function, a noninvasive means of detecting biochemical changes in redox status prior to irreversible functional changes is highly desirable for both early diagnosis and prognosis. We designed a novel F-labeled molecular probe, F-FPBT, for the direct detection of superoxide using positron emission tomography (PET). F-FPBT was radiosynthesized in one step by nucleophilic radiofluorination. , F-FPBT showed rapid and selective oxidation by superoxide (around 60% in 5 min) compared to other physiological ROS. In healthy mice and rats, F-FBPT is distributed to all major organs in the first few minutes post injection and is rapidly cleared via both renal and hepatobiliary routes with minimal background retention in the heart. In a rat model of doxorubicin-induced cardiotoxicity, F-FBPT showed significantly higher ( < 0.05) uptake in the hearts of treated animals compared to healthy controls. These results warrant further optimization of F-FBPT for clinical translation.
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http://dx.doi.org/10.1021/acs.molpharmaceut.1c00496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728736PMC
January 2022

Detecting Validated Intracellular ROS Generation with F-dihydroethidine-Based PET.

Mol Imaging Biol 2021 Nov 24. Epub 2021 Nov 24.

Division of Imaging Sciences and Biomedical Engineering, Kings College London, The Rayne Institute, St Thomas Hospital, London, SE1 7EH, UK.

Purpose: To determine the sensitivity of the F-radiolabelled dihydroethidine analogue ([F]DHE) to ROS in a validated ex vivo model of tissue oxidative stress.

Procedures: The sensitivity of [F]DHE to various ROS-generating systems was first established in vitro. Then, isolated rat hearts were perfused under constant flow, with contractile function monitored by intraventricular balloon. Cardiac uptake of infused [F]DHE (50-150 kBq.min) was monitored by γ-detection, while ROS generation was invoked by menadione infusion (0, 10, or 50 μm), validated by parallel measures of cardiac oxidative stress.

Results: [F]DHE was most sensitive to oxidation by superoxide and hydroxyl radicals. Normalised [F]DHE uptake was significantly greater in menadione-treated hearts (1.44 ± 0.27) versus control (0.81 ± 0.07) (p < 0.05, n = 4/group), associated with concomitant cardiac contractile dysfunction, glutathione depletion, and PKG1α dimerisation.

Conclusion: [F]DHE reports on ROS in a validated model of oxidative stress where perfusion (and tracer delivery) is unlikely to impact its pharmacokinetics.
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http://dx.doi.org/10.1007/s11307-021-01683-0DOI Listing
November 2021

Inter-phase 4D Cardiac MRI Registration with a Motion Prior Derived from CTA.

IEEE Trans Biomed Eng 2021 Nov 10;PP. Epub 2021 Nov 10.

Objective: Registration between phases in 4D cardiac MRI is essential for reconstructing high-quality images and appreciating the dynamics. Complex motion and limited image quality make it challenging to design regularization functionals. We propose to introduce a motion representation model (MRM) into a registration network to impose customized, site-specific, and spatially variant prior for cardiac motion.

Methods: We propose a novel approach to regularize deep registration with a DVF representation model using CTA. In the form of a convolutional auto-encoder, the MRM was trained to capture the spatially variant pattern of feasible DVF Jacobian. The CTA-derived MRM was then incorporated into an unsupervised network to facilitate MRI registration. In the experiment, 10 CTAs were used to derive the MRM. The method was tested on 10 0.35T scans in long-axis view with manual segmentation and 15 3T scans in short-axis view with tagging-based landmarks.

Results: Introducing the MRM improved registration accuracy and achieved 2.23, 7.21, and 4.42mm 80% Hausdorff distance on left ventricle, right ventricle, and pulmonary artery, respectively, and 2.23mm landmark registration error. The results were comparable to carefully tuned SimpleElastix, but reduced the registration time from 40 to 0.02s. The MRM presented good robustness to different DVF sample generation methods.

Conclusion: The model enjoys high accuracy as meticulously tuned optimization model and the efficiency of deep networks.

Significance: The method enables model to go beyond the quality limitation of MRI. The robustness to training DVF generation scheme makes the method attractive to adapting to the available data and software resources in various clinics.
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http://dx.doi.org/10.1109/TBME.2021.3127158DOI Listing
November 2021

Melatonin treatment delays ripening in mangoes associated with maintaining the membrane integrity of fruit exocarp during postharvest.

Plant Physiol Biochem 2021 Dec 29;169:22-28. Epub 2021 Oct 29.

College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China. Electronic address:

The effects of exogenous melatonin on postharvest ripening of mango (Mangifera indica L. cv. Keitt) were investigated after the fruit were dipped in 0 (as the control), 100, or 200 μM melatonin solution for 30 min, and then stored at room temperature (25 ± 1 °C). The results showed that melatonin treatments could delay the ripening process as indicated by inhibition to softening, respiration, color change and chlorophyll degradation in fruit during storage. Notably, 200 μM melatonin treatment delayed the degradation of phosphatidylglycerol (PG) and phosphatidylinositol (PI), and the accumulation of phosphatidylserine (PS) and phosphatidic acid (PA) in membrane phospholipids, inhibited the decrease in unsaturated fatty acids (IUFA) index and also decreased the contents of HO and malondialdehyde (MDA) in the exocarp of the fruit, which might collectively contribute to the integrity of the membrane associated with the delay in the ripening process of mango fruit during postharvest.
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http://dx.doi.org/10.1016/j.plaphy.2021.10.038DOI Listing
December 2021

A causal data fusion method for the general exposure and outcome.

Stat Med 2022 Jan 2;41(2):328-339. Epub 2021 Nov 2.

Department of Biostatistics, School of Public Health, Peking University, Beijing, P. R. China.

With the advent of the big data era, the need to combine multiple individual data sets to draw causal effects arises naturally in many medical and biological applications. Especially each data set cannot measure enough confounders to infer the causal effect of an exposure on an outcome. In this article, we extend the method proposed by a previous study to causal data fusion of more than two data sets without external validation and to a more general (continuous or discrete) exposure and outcome. Theoretically, we obtain the condition for identifiability of exposure effects using multiple individual data sources for the continuous or discrete exposure and outcome. The simulation results show that our proposed causal data fusion method has unbiased causal effect estimate and higher precision than traditional regression, meta-analysis and statistical matching methods. We further apply our method to study the causal effect of BMI on glucose level in individuals with diabetes by combining two data sets. Our method is essential for causal data fusion and provides important insights into the ongoing discourse on the empirical analysis of merging multiple individual data sources.
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http://dx.doi.org/10.1002/sim.9239DOI Listing
January 2022

Across demographics and recent history, most parents sing to their infants and toddlers daily.

Philos Trans R Soc Lond B Biol Sci 2021 Dec 1;376(1840):20210089. Epub 2021 Nov 1.

Department of Psychology, Harvard University, 33 Kirkland Street, Cambridge, MA 02138, USA.

Music is universally prevalent in human society and is a salient component of the lives of young families. Here, we studied the frequency of singing and playing recorded music in the home using surveys of parents with infants ( = 945). We found that most parents sing to their infant on a daily basis and the frequency of infant-directed singing is unrelated to parents' income or ethnicity. Two reliable individual differences emerged, however: (i) fathers sing less than mothers and (ii) as infants grow older, parents sing less. Moreover, the latter effect of child age was specific to singing and was not reflected in reports of the frequency of playing recorded music. Last, we meta-analysed reports of the frequency of infant-directed singing and found little change in its frequency over the past 30 years, despite substantial changes in the technological environment in the home. These findings, consistent with theories of the psychological functions of music, in general, and infant-directed singing, in particular, demonstrate the everyday nature of music in infancy. This article is part of the theme issue 'Voice modulation: from origin and mechanism to social impact (Part I)'.
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http://dx.doi.org/10.1098/rstb.2021.0089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558774PMC
December 2021

Joint Analysis of Genetic Correlation, Mendelian Randomization and Colocalization Highlights the Bi-Directional Causal Association Between Hypothyroidism and Primary Biliary Cirrhosis.

Front Genet 2021 4;12:753352. Epub 2021 Oct 4.

Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.

Hypothyroidism and primary biliary cirrhosis (PBC) are often co-existed in observational epidemiological studies. However, the causal relationship between them remains unclear. Genetic correlation, Mendelian randomization (MR) and colocalization analysis were combined to assess the potential causal association between hypothyroidism and PBC by using summary statistics from large-scale genome-wide association studies. Various sensitivity analyses had been conducted to assess the robustness and the consistency of the findings. The linkage disequilibrium score regression demonstrated significant evidence of shared genetic architecture between hypothyroidism and PBC, with the genetic correlation estimated to be 0.117 ( = 0.006). The OR of hypothyroidism on PBC was 1.223 (95% CI, 1.072-1.396; = 2.76 × 10) in MR analysis with inverse variance weighted (IVW) method. More importantly, the results from other 7MR methods with different model assumptions, were almost identical with that of IVW, suggesting the findings were robust and convincing. On the other hand, PBC was also causally associated with hypothyroidism (OR, 1.049; 95% CI, 1.010-1.089; = 0.012), and, again, similar results can also be obtained from other MR methods. Various sensitivity analyses regarding the outlier detection and leave-one-out analysis were also performed. Besides, colocalization analysis suggested that there existed shared causal variants between hypothyroidism and PBC, further highlighting the robustness of the results. Our results suggest evidence for the bi-directional causal association between hypothyroidism and PBC, which may provide insights into the etiology of hypothyroidism and PBC as well as inform prevention and intervention strategies directed toward both diseases.
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http://dx.doi.org/10.3389/fgene.2021.753352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521021PMC
October 2021

Enzyme Therapeutic for Ischemia and Reperfusion Injury in Organ Transplantation.

Adv Mater 2022 Jan 6;34(1):e2105670. Epub 2021 Nov 6.

Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.

Ischemia-reperfusion injury (IRI) remains as a critical challenge for organ transplantation. Herein, an enzyme therapeutic based on superoxide dismutase and catalase for effective mitigation of IRI and pathogen-induced liver injury is reported, providing a therapeutic for organ transplantation and other diseases.
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http://dx.doi.org/10.1002/adma.202105670DOI Listing
January 2022

Metformin attenuates angiotensin II-induced cardiomyocyte hypertrophy by upregulating the MuRF1 and MAFbx pathway.

Exp Ther Med 2021 Nov 31;22(5):1231. Epub 2021 Aug 31.

Department of Cardiology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, P.R. China.

Pathological cardiac hypertrophy induced by aging and neurohumoral activation, such as angiotensin II (Ang II) activation, is an independent risk factor for heart failure. The muscle really interesting new gene-finger protein-1 (MuRF1) and muscle atrophy F-box (MAFbx) pathway has been previously reported to be an important mechanism underlying the pathogenesis of cardiac hypertrophy. Metformin is currently the first-line blood glucose-lowering agent that can be useful for the treatment of cardiovascular diseases. However, the potential role of metformin in the modulation of MuRF1 and MAFbx in cardiomyocyte hypertrophy remains poorly understood. The present study used H9c2 cells, a cardiomyocyte cell model. The surface area of cultured rat H9c2 myoblasts was measured and the expression levels of MuRF1 and MAFbx were quantified using western blot or reverse transcription-quantitative PCR. H9c2 cells were transfected with MuRF1 and MAFbx small interfering (si) RNA. The present study revealed that Ang II treatment significantly increased the cell surface area of model cardiomyocytes. Additionally, atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) mRNA and protein expression was increased following this treatment. Ang II also downregulated MuRF1 and MAFbx protein and mRNA expression. In the H9C2, treatment with metformin attenuated hypertrophic remodeling. In addition, expression of ANP and BNP was significantly reduced in metformin-treated H9C2 cells. The results indicated that metformin increased the activity of MuRF1 and MAFbx and upregulated their expression, the knockdown of which resulted in deteriorative Ang II-induced cell hypertrophy, even following treatment with metformin. Taken together, data from the present study suggest that metformin can prevent cardiac hypertrophy through the MuRF1 and MAFbx pathways.
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http://dx.doi.org/10.3892/etm.2021.10665DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438677PMC
November 2021

Single-Incision vs. Conventional Laparoscopic Surgery for Colorectal Cancer: An Update of a Systematic Review and Meta-Analysis.

Front Surg 2021 23;8:704986. Epub 2021 Aug 23.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Although the advantages of single-incision laparoscopic surgery have been reported in several meta-analyses, the low quality of studies included in the meta-analyses limits the reliability of such a conclusion. In recent years, the number of randomized controlled trials on the efficacy of SILS in colorectal cancer has been on the rise. This update systematic review and meta-analysis of RCTs aims to compare efficacy and safety of SILS and CLS in the patients with colorectal cancer. Relevant data was searched on the CNKI, Wanfang, VIP, Sinomed, PubMed, Embase, and Cochrane CENTRAL databases from inception until February 5th, 2021. All RCTs comparing SILS and CLS were included. The main outcomes were 30 days of mortality, postoperative complications, intraoperative complications, whereas secondary outcomes were the number of lymph nodes removed, duration of hospital stay, intraoperative blood loss, abdominal incision length, reoperation, readmission, conversion to laparotomy, operation time and anastomotic leakage. A total of 10 RCTs were included, involving 1,133 participants. The quality of the included studies was generally high. No significant difference was found between SILS and CLS in the 30 days mortality rate. The results showed that SILS group had a lower rate of postoperative complications (RR = 0.67, 95% CI: 0.49-0.92), higher rate of intraoperative complications (RR = 2.26, 95%CI: 1.00-5.10), shorter length of abdominal incision (MD = -2.01, 95% CI:-2.42-1.61) (cm), longer operation time (MD = 11.90, 95% CI: 5.37-18.43) (minutes), shorter hospital stay (MD = -1.12, 95% CI: -1.89-0.34) (days) compared with CLS group. However, intraoperative blood loss (MD = -8.23, 95% CI: -16.75-0.29) (mL), number of lymph nodes removed (MD = -0.17, 95% CI: -0.79-0.45), conversion to laparotomy (RR=1.31, 95% CI: 0.48-3.60), reoperation (RR = 1.00, 95% CI: 0.30-3.33) and readmission (RR =1.15, 95% CI: 0.12-10.83) and anastomotic leakage were not significantly different between the two groups. These results indicate that SILS did not has a comprehensive and obvious advantage over the CLS. Surgeons and patients should carefully weigh the pros and cons of the two surgical procedures. Further RCTs are needed to prove long-term outcomes of SILS in colorectal cancer.
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http://dx.doi.org/10.3389/fsurg.2021.704986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419430PMC
August 2021

Genetically Predicted Insomnia in Relation to 14 Cardiovascular Conditions and 17 Cardiometabolic Risk Factors: A Mendelian Randomization Study.

J Am Heart Assoc 2021 08 28;10(15):e020187. Epub 2021 Jul 28.

Department of Biostatistics School of Public Health Cheeloo College of MedicineShandong University Jinan Shandong China.

Background This Mendelian randomization study aims to investigate causal associations between genetically predicted insomnia and 14 cardiovascular diseases (CVDs) as well as the potential mediator role of 17 cardiometabolic risk factors. Methods and Results Using genetic association estimates from large genome-wide association studies and UK Biobank, we performed a 2-sample Mendelian randomization analysis to estimate the associations of insomnia with 14 CVD conditions in the primary analysis. Then mediation analysis was conducted to explore the potential mediator role of 17 cardiometabolic risk factors using a network Mendelian randomization design. After correcting for multiple testing, genetically predicted insomnia was consistent significantly positively associated with 9 of 14 CVDs, those odds ratios ranged from 1.13 (95% CI, 1.08-1.18) for atrial fibrillation to 1.24 (95% CI, 1.16-1.32) for heart failure. Moreover, genetically predicted insomnia was consistently associated with higher body mass index, triglycerides, and lower high-density lipoprotein cholesterol, each of which may act as a mediator in the causal pathway from insomnia to several CVD outcomes. Additionally, we found very little evidence to support a causal link between insomnia with abdominal aortic aneurysm, thoracic aortic aneurysm, total cholesterol, low-density lipoprotein cholesterol, glycemic traits, renal function, and heart rate increase during exercise. Finally, we found no evidence of causal associations of genetically predicted body mass index, high-density lipoprotein cholesterol, or triglycerides on insomnia. Conclusions This study provides evidence that insomnia is associated with 9 of 14 CVD outcomes, some of which may be partially mediated by 1 or more of higher body mass index, triglycerides, and lower high-density lipoprotein cholesterol.
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http://dx.doi.org/10.1161/JAHA.120.020187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475657PMC
August 2021

Primary Pleural Squamous Cell Carcinoma, Highly Positive PD-L1, Shows Marked Response to Camrelizumab: A Case Report.

Clin Med Insights Oncol 2021 12;15:11795549211028571. Epub 2021 Jul 12.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, China.

Here, we reported the rare case of primary pleural squamous cell carcinoma (PPSCC) in a 71-year-old male patient. After chemo and targeted therapies, the patient showed continuous tumor progression and clinical deterioration. Fortunately, the patient had a high expression level of PD-L1 (80%) in the tumor tissues. Ultimately, the patient survived for additional 6 months with camrelizumab treatment. In summary, camrelizumab may be a good candidate for the treatment of PPSCC, especially in tumors with high PD-L1 expression.
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http://dx.doi.org/10.1177/11795549211028571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278452PMC
July 2021

Intravenous thrombolysis in Chinese patients with mild acute ischemic stroke.

Ann Transl Med 2021 May;9(9):767

Vascular Neurology, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: Mild stroke accounts for more than a half of all stroke patients, and short-term outcomes after treatment with intravenous (IV) recombinant tissue plasminogen activator (rtPA) have not been fully investigated in this group.

Methods: Our study investigated short-term outcomes and predictors for a favorable functional outcome at discharge in mild stroke patients with IV rtPA. 6,752 mild stroke patients in the China Stroke Center Alliance with a clinical diagnosis of acute ischemic stroke, within 4.5 hours from symptom onset, with a baseline National Institutes of Health Stroke Scale score ≤5 and received rt-PA treatment were included in this retrospective analysis. Univariable and multivariable analyses were performed to identify factors independently associated with a favorable functional outcome.

Results: Only 18.5% had an unfavorable functional outcome at discharge, 91.1% were discharged home, 89.9% could ambulate independently, 95.9% had a length of stay of 3 days or longer and 1.9% had sICH. A multivariable Logistic regression model identified that age >80 years [adjusted odds ratio (aOR): 1.57 (1.1-2.25)], diabetes mellitus [aOR: 1.35 (1.16-1.58)], 3-4.5 h time window [aOR: 1.43 (1.26-1.63)] and NIHSS score [3 0, aOR: 1.49 (1.05-2.11); 4 0, aOR: 2.36 (1.68-3.33); 5 0, aOR: 2.51 (1.77-3.56)] were independent risk factors for mRS >2 with hospital region, hospital level and hypertension as covariates.

Conclusions: Our findings suggest that tPA is safe and effective in mild stroke patients with age ≤80 within the 3 hour time window and in those without diabetes mellitus, further studies are needed to confirm the findings.
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http://dx.doi.org/10.21037/atm-21-40DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246215PMC
May 2021

Dietary Fibre Intervention for Gut Microbiota, Sleep, and Mental Health in Adults with Irritable Bowel Syndrome: A Scoping Review.

Nutrients 2021 Jun 23;13(7). Epub 2021 Jun 23.

School of Medical and Health Sciences, Edith Cowan University, Joondalup Drive, Perth 6027, Australia.

Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder affecting 4-5% of the global population. This disorder is associated with gut microbiota, diet, sleep, and mental health. This scoping review therefore aims to map existing research that has administrated fibre-related dietary intervention to IBS individuals and reported outcomes on at least two of the three following themes: gut microbiota, sleep, and mental health. Five digital databases were searched to identify and select papers as per the inclusion and exclusion criteria. Five articles were included in the assessment, where none reported on all three themes or the combination of gut microbiota and sleep. Two studies identified alterations in gut microbiota and mental health with fibre supplementation. The other three studies reported on mental health and sleep outcomes using subjective questionnaires. IBS-related research lacks system biology-type studies targeting gut microbiota, sleep, and mental health in patients undergoing diet intervention. Further IBS research is required to explore how human gut microbiota functions (such as short-chain fatty acids) in sleep and mental health, following the implementation of dietary pattern alteration or component supplementation. Additionally, the application of objective sleep assessments is required in order to detect sleep change with more accuracy and less bias.
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http://dx.doi.org/10.3390/nu13072159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308461PMC
June 2021

A novel non-enzymatic glucose electrochemical sensor with high sensitivity and selectivity based on CdInOnanoparticles on 3D Ni foam substrate.

Nanotechnology 2021 Jul 14;32(40). Epub 2021 Jul 14.

Key Lab of Quantum Information of Yunnan Province, Yunnan University, 650091 Kunming, People's Republic of China.

Due to the poor conductivity of Fe based, Cu based and Co based electrode materials commonly used in the electrochemical detection of glucose, and the uneven stirring and poor conductivity of the traditional preparation method based on glassy carbon electrode. In order to solve the above problems, in this work, CdInOwith high electrical conductivity was directly grown on three-dimensional (3D) Ni foam to prepare electrode materials for non-enzymatic glucose sensors. CdInOnanoparticles is prepared from cadmium acetate and indium nitrate hydrate in benzyl alcohol by non-aqueous sol-gel method. The electrocatalytic oxidation performances of CdInOelectrode material for non-enzymatic glucose are studied. The results show that the proposed CdInOelectrode material has good electrochemical properties and sensing performance for glucose detection. The electrochemical response of CdInOelectrode material to glucose is recorded that calibration plot for glucose concentrations ranging from 1.0M to 1.0 mM ( = 0.99), a limit detection of 0.08M, an excellent sensitivity of 3.2925 mA.mM.cm, a rapid response time of 1.58 s, a good selectivity and a good long-term stability. These demonstrate the significant potential of CdInOelectrode material based on 3D Ni foam as non-enzymatic glucose sensors, which makes it possible to use it as a practical glucose detector. This work could introduce a new concept of nanoparticles modified electrode material grown directly on 3D Ni foam, thus a simple and reliable electrochemical glucose sensor platform is realized. This study was completed in 2019 in the school of materials and energy, Yunnan University.
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http://dx.doi.org/10.1088/1361-6528/ac0fa2DOI Listing
July 2021

Protocol for a pharmacogenomic study on individualised antipsychotic drug treatment for patients with schizophrenia.

BJPsych Open 2021 Jun 29;7(4):e121. Epub 2021 Jun 29.

Institute of Mental Health, The Sixth Hospital of Peking University, China; and Key Laboratory of Mental Health, Ministry of Health & National Clinical Research Center for Mental Disorders (Peking University), China.

Background: Schizophrenia is a severe and complex psychiatric disorder that needs treatment based on extensive experience. Antipsychotic drugs have already become the cornerstone of the treatment for schizophrenia; however, the therapeutic effect is of significant variability among patients, and only around a third of patients with schizophrenia show good efficacy. Meanwhile, drug-induced metabolic syndrome and other side-effects significantly affect treatment adherence and prognosis. Therefore, strategies for drug selection are desperately needed. In this study, we will perform pharmacogenomics research and set up an individualised preferred treatment prediction model.

Aims: We aim to create a standard clinical cohort, with multidimensional index assessment of antipsychotic treatment for patients with schizophrenia.

Method: This trial is designed as a randomised clinical trial comparing treatment with different kinds of antipsychotics. A total sample of 2000 patients with schizophrenia will be recruited from in-patient units from five clinical research centres. Using a computer-generated program, the participants will be randomly assigned to four treatment groups: aripiprazole, olanzapine, quetiapine and risperidone. The primary outcomes will be measured as changes in the Positive and Negative Syndrome Scale of schizophrenia, which reflects the efficacy. Secondary outcomes include the measure of side-effects, such as metabolic syndromes. The efficacy evaluation and side-effects assessment will be performed at baseline, 2 weeks, 6 weeks and 3 months.

Results: This trial will assess the efficacy and side effects of antipsychotics and create a standard clinical cohort with a multi-dimensional index assessment of antipsychotic treatment for schizophrenia patients.

Conclusion: This study aims to set up an individualized preferred treatment prediction model through the genetic analysis of patients using different kinds of antipsychotics.
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http://dx.doi.org/10.1192/bjo.2021.945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269926PMC
June 2021

Sinapic Acid Reduces Oxidative Stress and Pyroptosis via Inhibition of BRD4 in Alcoholic Liver Disease.

Front Pharmacol 2021 4;12:668708. Epub 2021 Jun 4.

Department of Pharmacy, The Second Hospital of Dalian Medical University, Dalian, China.

Alcoholic liver disease (ALD) is one of the main causes of death in chronic liver disease. Oxidative stress and pyroptosis are important factors leading to ALD. Bromodomain-containing protein 4 (BRD4) is a factor that we have confirmed to regulate ALD. As a phenolic acid compound, sinapic acid (SA) has significant effects in antioxidant, anti-inflammatory and liver protection. In this study, we explored whether SA regulates oxidative stress and pyroptosis through BRD4 to play a protective effect in ALD. Male C57BL/6 mice and AML-12 cells were used for experiments. We found that SA treatment largely abolished the up-regulation of BRD4 and key proteins of the canonical pyroptosis signalling in the liver of mice fed with alcohol, while conversely enhanced the antioxidant response. Consistantly, both SA pretreatment and BRD4 knockdown inhibited oxidative stress, pyroptosis, and liver cell damage . More importantly, the expression levels of BRD4 and pyroptosis indicators increased significantly in ALD patients. Molecule docking analysis revealed a potent binding of SA with BRD4. In conclusion, this study demonstrates that SA reduces ALD through BRD4, which is a valuable lead compound that prevents the ALD process.
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http://dx.doi.org/10.3389/fphar.2021.668708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212038PMC
June 2021

Identification of microenvironment related potential biomarkers of biochemical recurrence at 3 years after prostatectomy in prostate adenocarcinoma.

Aging (Albany NY) 2021 06 16;13(12):16024-16042. Epub 2021 Jun 16.

Department of Biostatistics, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China.

Prostate adenocarcinoma is one of the leading adult malignancies. Identification of multiple causative biomarkers is necessary and helpful for determining the occurrence and prognosis of prostate adenocarcinoma. We aimed to identify the potential prognostic genes in the prostate adenocarcinoma microenvironment and to estimate the causal effects simultaneously. We obtained the gene expression data of prostate adenocarcinoma from TCGA project and identified the differentially expressed genes based on immune-stromal components. Among these genes, 68 were associated with biochemical recurrence at 3 years after prostatectomy in prostate adenocarcinoma. After adjusting for the minimal sets of confounding covariates, 14 genes (, and ) related to the microenvironment were identified as prognostic biomarkers using the targeted maximum likelihood estimation. Both the average and individual causal effects were obtained to measure the magnitude of the effect. CIBERSORT and gene set enrichment analyses showed that these prognostic genes were mainly associated with immune responses. and were correlated with androgen receptor expression, a main driver of prostate adenocarcinoma progression. Finally, five genes were validated in another prostate adenocarcinoma cohort (GEO: GSE70770). These findings might lead to the improved prognosis of prostate adenocarcinoma.
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http://dx.doi.org/10.18632/aging.203121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266350PMC
June 2021

Hyperhomocysteinemia exacerbates ischemia-reperfusion injury-induced acute kidney injury by mediating oxidative stress, DNA damage, JNK pathway, and apoptosis.

Open Life Sci 2021 29;16(1):537-543. Epub 2021 May 29.

Division of Nephrology, Guizhou Provincial People's Hospital, Guizhou Provincial Institute of Nephritic & Urinary Disease, No. 83 East ZhongShan Road, Guiyang 550002, Guizhou, People's Republic of China.

Background: Hyperhomocysteinemia (HHcy) plays an important role in the progression of many kidney diseases; however, the relationship between HHcy and ischemia-reperfusion injury (IRI)-induced acute kidney injury (IRI-induced AKI) is far from clear. In this study, we try to investigate the effect and possible mechanisms of HHcy on IRI-induced AKI.

Methods: Twenty C57/BL6 mice were reared with a regular diet or high methionine diet for 2 weeks (to generate HHcy mice); after that, mice were subgrouped to receive sham operation or ischemia-reperfusion surgery. Twenty four hour after reperfusion, serum creatinine, blood urea nitrogen, and Malondialdehyde (MDA) were measured. H&E staining for tubular injury, western blot for γH2AX, JNK, p-JNK, and cleaved caspase 3, and TUNEL assay for tubular cell apoptosis were also performed.

Results: Our results showed that HHcy did not influence the renal function and histological structure, as well as the levels of MDA, γH2AX, JNK, p-JNK, and tubular cell apoptosis in control mice. However, in IRI-induced AKI mice, HHcy caused severer renal dysfunction and tubular injury, higher levels of oxidative stress, DNA damage, JNK pathway activation, and tubular cell apoptosis.

Conclusion: Our results demonstrated that HHcy could exacerbate IRI-induced AKI, which may be achieved through promoting oxidative stress, DNA damage, JNK pathway activation, and consequent apoptosis.
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http://dx.doi.org/10.1515/biol-2021-0054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165256PMC
May 2021

The Analgesic Benefits of Ketorolac to Local Anesthetic Wound Infiltration Is Statistically Significant But Clinically Unimportant: A Comprehensive Systematic Review and Meta-Analysis.

Adv Wound Care (New Rochelle) 2021 11 5;10(11):583-595. Epub 2021 Jul 5.

TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Even though ketorolac-infiltration is said to provide superior postoperative analgesic benefits in different surgical procedures, its safety and efficacy remain to be validated because of the lack of high-quality evidence. We aimed to summarize the efficacy and safety of ketorolac-infiltration based on published randomized-controlled trials (RCTs). This work followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, assessing the methodological quality of systematic reviews and the Cochrane Collaboration recommendations. We searched for RCTs evaluating the efficacy of ketorolac-infiltration in adults in the PubMed, Web of Science, Embase, Cochrane Library, Chinese databases, and Google Scholar. The two co-primary outcomes of this meta-analysis were rescue analgesic consumption in the 24-h postoperative period and rest pain scores. Twelve trials (761 patients) were analyzed. Ketorolac-infiltration provided a clinically unimportant benefit in morphine consumption (mean difference, -2.81 mg; 95% confidence interval [CI], -5.11 to -0.50;  = 0.02; moderate-quality evidence). Low-to-moderate quality evidence supported a brief (2-6 h), clinically subtle, but statistically consistent effect of surgical site ketorolac-infiltration in reducing wound pain at rest. High-quality evidence supported shorter hospital stays for surgical patients receiving local ketorolac-infiltration when compared to controls (mean difference, -0.12 days; 95% CI, -0.17 to -0.08;  < 0.00001). Further, ketorolac-infiltration does not improve any opioid-related side effects. Ketorolac-infiltration provides statistically significant but clinically unimportant benefits for improving postoperative wound pain. Overall, despite the fact that current moderate-to-high quality of evidence does not support routine using of ketorolac as an adjuvant to local anesthetic for wound infiltration, these findings underscore the importance of optimizing agents and sustained delivery parameters in postoperative local anesthetic practice. Clinical Trials.gov ID: CRD42021229095.
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http://dx.doi.org/10.1089/wound.2021.0067DOI Listing
November 2021

One-dimensional InOnanorods as sensing material for ppb-level n-butanol detection.

Nanotechnology 2021 Jun 22;32(37). Epub 2021 Jun 22.

School of Materials and Energy, Yunnan University, Kunming 650091, People's Republic of China.

Effectively and quantificationally detecting hazardous gas n-butanol is very significant in daily life, which can bring about a safe living condition for humans. In this study, the one-dimensional InOnanorods were successfully synthesized via hydrothermal route and post-heat treatment. Noticeably, one-dimensional nanorods structures were obtained and the products presented a superior growth orientation along with (222) plane. Additionally, systematical gas-sensing measurements of the sensor made from InOnanorods towards hazardous n-butanol gas were conducted. Results exhibited that the fabricated sensor showed excellent n-butanol sensing properties, with aspects to a superior response value of 342.20 with concentration 100 ppm at 240 °C, remarkable selectivity, fast response/recovery times (77.5/34.2 s) and good stability. More interestingly, the detection limit of sensor as low as 500 ppb and a good linearity relationship between response values and n-butanol concentrations was presented. Gas-sensitive properties of this sensor are better than previously reported in n-butanol detection. All results demonstrate that one-dimensional InOnanorod is a promising sensor material to practical applications in effectively detecting n-butanol gas.
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http://dx.doi.org/10.1088/1361-6528/ac06f6DOI Listing
June 2021

An Additive Consistency and Consensus Approach for Group Decision Making With Probabilistic Hesitant Fuzzy Linguistic Preference Relations and Its Application in Failure Criticality Analysis.

IEEE Trans Cybern 2021 May 25;PP. Epub 2021 May 25.

In this article, probabilistic hesitant fuzzy linguistic preference relations (PHFLPRs) are proposed to present the qualitative pairwise preference information of decision makers (DMs) with hesitation and probability uncertainty assessments. The measurements and improvements of additive consistency and consensus of PHFLPRs are investigated in group decision making (GDM). First, a new concept of probabilistic hesitant fuzzy linguistic term sets is defined. Second, the consistency and consensus measurements are established to survey the additive consistency and consensus levels of PHFLPRs. Subsequently, an optimization model is developed to improve the unacceptably additive consistent PHFLPR. By optimizing the unacceptable consensual PHFLPRs with repeating additive consistency improvement, the acceptably additive consistent and consensual PHFLPRs are obtained, based on which DMs' weights are determined objectively and then, the collective PHFLPR is aggregated from individual PHFLPRs. Alternatives' priority weights are derived from the collective PHFLPR as GDM. Finally, an example about failure criticality analysis is given, and a comparison analysis is presented.
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http://dx.doi.org/10.1109/TCYB.2021.3072364DOI Listing
May 2021

Temperature self-calibrated pH sensor based on GO/PVA-coated MZI cascading FBG.

Opt Express 2021 Apr;29(9):13530-13541

A temperature self-calibrated potential of hydrogen (pH) sensor based on the single mode fiber-tapered dual core photonic crystal fiber-single mode fiber (SMF-TDCPCF-SMF) structure cascaded with a fiber Bragg grating (FBG) is proposed and demonstrated. The TDCPCF structure formed Mach-Zehnder interferometer (MZI) is modified with a coating of graphene oxide/polyvinyl alcohol (GO/PVA) hybrid hydrogel to realize the measurement of pH, and the uncoated FBG is used to calibrate temperature. In our experiment, the sensitivity coefficient of 0.69 nm/pH with R=0.99 and the hysteresis loss of less than 0.007 are achieved within the pH range from pH 4.00 to pH 9.85. The measured response time from pH 7.00 to pH 4.00, 6.00 and 9.85 are no higher than 10s. Moreover, the resonant wavelengths of MZI and FBG also exhibit good linear relationship with the temperature sensitivity coefficient of 0.15 nm/°C (R=0.99) and 0.09 nm/°C (R=0.97) respectively. It is demonstrated successfully that the proposed sensor has broad application prospects in the field of environmental monitoring, biological sensing and chemical analysis, due to the good performance of the temperature self-calibrated pH monitoring, repeatability, linearity, response time and reversibility.
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http://dx.doi.org/10.1364/OE.421782DOI Listing
April 2021

Dosimetric impact from cardiac motion to heart substructures in thoracic cancer patients treated with a magnetic resonance guided radiotherapy system.

Phys Imaging Radiat Oncol 2021 Jan 18;17:8-12. Epub 2020 Dec 18.

Department of Radiation Oncology, University of California, Los Angeles, CA, USA.

Few studies have examined the cardiac volume and radiation dose differences among cardiac phases during radiation therapy (RT). Such information is crucial to dose reconstruction and understanding of RT related cardiac toxicity. In a cohort of nine patients, we studied the changes in the volume and doses of several cardiac substructures between the end-diastolic and end-systolic phases based on the clinical magnetic resonance-guided RT (MRgRT) treatment plans. Significant differences in the volume and dose between the two phases were observed. Onboard cardiac cine MRI holds promise for patient-specific cardiac sparing treatment designs.
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http://dx.doi.org/10.1016/j.phro.2020.11.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057956PMC
January 2021
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