Publications by authors named "Ran Ding"

101 Publications

[Association of different stages of histological chorioamnionitis with respiratory distress syndrome in preterm infants with a gestational age of < 32 weeks].

Zhongguo Dang Dai Er Ke Za Zhi 2021 Mar;23(3):248-253

Qingdao Women and Children's Hospital, Qingdao University, Qingdao, Shangdong 266000, China.

Objective: To study the association of different stages of histological chorioamnionitis (HCA) with the incidence rate and severity of respiratory distress syndrome (RDS) in preterm infants.

Methods: Related data were collected from the infants and their mothers who were treated in the Neonatal Intensive Care Unit of Qingdao Women and Children's Hospital, Qingdao University, from January 2018 to June 2020. According to the presence or absence of HCA and its stage, the infants were divided into four groups: control (=109), early-stage HCA (=126), middle-stage HCA (=105), and late-stage HCA (=36). The four groups were compared in terms of gestational age, birth weight, sex, maternal age, placental abruption, prenatal use of antibiotics, and incidence rate of RDS. The correlation between HCA stage and RDS severity was analyzed.

Results: Compared with the control and late-stage HCA groups, the early-stage HCA group had a significantly lower incidence rate of placental abruption and a significantly higher rate of prenatal use of antibiotics ( < 0.05), and the early-stage HCA group had a significantly lower incidence rate of RDS than the control group ( < 0.05). The multivariate logistic regression analysis showed that early-, middle-, and late-stage HCA were protective factors against RDS ( < 0.05). The Spearman test showed that the severity of RDS in preterm infants was not correlated with the HCA stage ( > 0.05).

Conclusions: Early-, middle-, and late-stage HCA can reduce the incidence rate of RDS in preterm infants. HCA stage may not be correlated with RDS severity in preterm infants, which needs to be verified by further research.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969184PMC
March 2021

P2X4 receptor in the dorsal horn contributes to BDNF/TrkB and AMPA receptor activation in the pathogenesis of remifentanil-induced postoperative hyperalgesia in rats.

Neurosci Lett 2021 04 24;750:135773. Epub 2021 Feb 24.

Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin Research Institute of Anesthesiology, Tianjin, 300052, China. Electronic address:

The mechanism underlying the high incidence of remifentanil-induced postoperative hyperalgesia is unclear. Also, no effective prevention method exists. Inflammatory pain-related studies showed that P2X4 purinergic receptors (P2X4Rs) in the dorsal horn of the spinal cord and dorsal root ganglia are essential for maintaining allodynia caused by inflammation. However, little is known about its role in opioid-induced hyperalgesia. This study aimed to determine the role of P2X4R and related signaling pathways in the remifentanil-induced postoperative hyperalgesia (RIH) model. The study simulated the remifentanil infusion and surgical incision during general anesthesia. The mRNA and protein expression level of P2X4R in rats with RIH model increased from 2 h to 48 h after the surgery. The administration of P2X4R inhibitors prevented the occurrence of RIH, resulting in a reduction in mechanical and thermal pain. Moreover, P2X4R was involved in RIH in male and female rats, indicating no sex-specific difference. P2X4R also increased the expression of AMPA receptor subunit GluA1 in a brain-derived neurotrophic factor (BDNF) / tyrosine receptor kinase B (TrkB) dependent manner. The results from whole-cell patch-clamp recording suggested that P2X4R also regulated AMPA receptor-mediated miniature excitatory postsynaptic currents and participated in the synaptic plasticity of spinal dorsal horn neurons. In summary, P2X4R was involved in AMPAR expression, electrophysiological function, and synaptic plasticity of spinal dorsal horn neurons through BDNF/TrkB signaling. This might be the mechanism underlying RIH, and hence inhibition of P2X4R might be a potential treatment strategy.
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http://dx.doi.org/10.1016/j.neulet.2021.135773DOI Listing
April 2021

Sleep Deprivation Aggravates Cognitive Impairment by the Alteration of Hippocampal Neuronal Activity and the Density of Dendritic Spine in Isoflurane-Exposed Mice.

Front Behav Neurosci 2020 23;14:589176. Epub 2020 Nov 23.

Laboratory of Neurobiology, School of Biomedical Engineering, Tianjin Medical University, Tianjin, China.

Isoflurane contributes to cognitive deficits when used as a general anesthetic, and so does sleep deprivation (SD). Patients usually suffer from insomnia before an operation due to anxiety, fear, and other factors. It remains unclear whether preoperative SD exacerbates cognitive impairment induced by isoflurane. In this study, we observed the effects of pretreated 24-h SD in adult isoflurane-exposed mice on the cognitive behaviors, the Ca signals of dorsal hippocampal CA1 (dCA1) neurons with fiber photometry, and the density of dendritic spines in hippocampal neurons. Our results showed that in cognitive behavior tasks, short-term memory damages were more severe with SD followed by isoflurane exposure than that with SD or isoflurane exposure separately, and interestingly, severe long-term memory deficits were induced only by SD followed by isoflurane exposure. Only the treatment of SD followed by isoflurane exposure could reversibly decrease the amplitude of Ca signals when mice were freely moving and increase the duration of Ca signals during the long-term memory behavior test. The density of dendritic spines with both SD and isoflurane exposure was lower than that with SD alone. This study suggests that SD should be avoided preoperatively in patients undergoing elective surgery under isoflurane anesthesia.
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http://dx.doi.org/10.3389/fnbeh.2020.589176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719754PMC
November 2020

Theoretical Investigations on the Excited-State Dynamics of an Al Fluorescence Sensor.

J Phys Chem A 2020 Dec 16;124(52):11093-11101. Epub 2020 Dec 16.

College of Chemical and Materials Engineering, Anhui Science and Technology University, Fengyang 233100, P. R. China.

Twisted internal charge transfer (TICT) states are of fundamental importance during the photo-physical processes of dyes and sensors. In this contribution, excited-state dynamics of an Al fluorescence sensor 1-{[(2-hydroxyphenyl)-imino]methyl}naphthalen-2-ol based on the turn-on signal is clarified. Two different dark TICT states are observed by exploring the excited-state potential energy surface. With the twist of the C2-N bond, the two dark states can be reached facilely, which induce the experimentally observed weak fluorescence of the sensor. The sensing mechanism is then uncovered by investigating the electronic coupling between the sensor and analyte. Al is proved to form strong coordination bonds with the sensor, which restricts the motion of the C2-N bond. Consequently, the TICT states are eliminated, which generate the turn-on signal. This sensing mechanism is trustworthy and intrinsically different from the previously proposed one, which would shed some light on the design of turn-on sensors.
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http://dx.doi.org/10.1021/acs.jpca.0c09977DOI Listing
December 2020

The facile and controllable synthesis of a bacterial cellulose/polyhydroxybutyrate composite by co-culturing Gluconacetobacter xylinus and Ralstonia eutropha.

Carbohydr Polym 2021 Jan 28;252:117137. Epub 2020 Sep 28.

State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan, 430070, PR China; School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan, 430070, PR China; School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, 430070, PR China. Electronic address:

Bacterial cellulose (BC) and polyhydroxybutyrate (PHB) are microbial polymers considered to be promising biodegradable alternatives to fossil fuel derivatives. BC and PHB can be combined into a composite with enhanced mechanical properties. The synthesis processes of BC/PHB composites described until now are complicated with multiple steps. Here, BC/PHB composites were synthesized by a facile Gluconacetobacter xylinus and Ralstonia eutropha co-culture method generating BC and PHB simultaneously in situ. This co-culture approach ensured a certain level of control over the synthesis process. By simply varying the R. eutropha inoculum, the weight ratio of PHB into BC/PHB was adjusted from 15.62 to 42.88 %. The fabricated composites were networks of BC fibers connecting PHB particles. BC/PHBs were characterized by thermal and mechanical analyses and exhibited a 2.6 times higher capacity for toxic copper adsorption than pure BC. The co-culture technique described here is a simple synthesis method to obtain BC/PHB with adjustable characteristics.
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http://dx.doi.org/10.1016/j.carbpol.2020.117137DOI Listing
January 2021

Crystalline CdS/MoS shape-controlled by a bacterial cellulose scaffold for enhanced photocatalytic hydrogen evolution.

Carbohydr Polym 2020 Dec 13;250:116909. Epub 2020 Aug 13.

State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070, PR China; School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, PR China; School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, PR China. Electronic address:

The conversion of sunlight into H by noble-metal-free photocatalysts is a promising approach for the production of easy-to-store chemical energy. For this purpose, higher efficiency is achieved by photocatalysts with heterojunctions preventing fast charge recombination. Most processes for the synthesis of high-performance heterojunction photocatalysts require solvents harmful to living organisms. Here, berry-shaped (b)-CdS/MoS particles were fabricated instead by a hydrothermal process where non-toxic bacterial cellulose was used to mold b-CdS into nanostructures with enhanced spatial arrangement. Subsequently, MoS was combined with b-CdS resulting in a composite with suitable shape and intimate semiconductor contacts beneficial for charge transfer. The photocatalytic H evolution (PHE) of b-CdS/1%MoS was 63.59 mmol g h. It was 61.1 times, 397 times, and 10.2 times higher than PHE with b-CdS, CdS fabricated without BC scaffold, and b-CdS doped with Pt, respectively. These results show the high potential of b-CdS/MoS and the associated synthesis method for PHE.
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http://dx.doi.org/10.1016/j.carbpol.2020.116909DOI Listing
December 2020

The relationship between platelet indices and patent ductus arteriosus in preterm infants: a systematic review and meta-analysis.

Eur J Pediatr 2021 Mar 19;180(3):699-708. Epub 2020 Sep 19.

Department of NICU, Qingdao Women and Children's Hospital, Qingdao University, Shibei District, Qingdao, 266000, Shandong province, China.

Patent ductus arteriosus (PDA), one of the most common disorders in newborns, is associated with many complications in premature infants such as respiratory distress syndrome (RDS) and bronchopulmonary dysplasia (BPD). However, the diagnosis of hemodynamically significant patent ductus arteriosus (hsPDA) is still an ongoing debate. The relationship between platelet parameters and hsPDA has been explored in many studies over the last decade, but there is still no definite conclusion. We aim to explain the relationship between platelet parameters and hsPDA through this meta-analysis. Therefore, we used PubMed, Embase, the Cochrane Library, and Web of Science databases as well as the Google Scholar to search for studies up to May 2020. Three reviewers independently screened the articles, evaluated the quality of the articles, and collected the data. The random-effects model and fixed-effects model were used to evaluate pooled results. We used the I-square (I) test to examine heterogeneity and the funnel plot; Egger's test and meta-regression analysis were used to test for publication bias. Influence analysis was also carried out in this study. Stata version 12.0 software was used for data analysis. Fourteen studies, which included 3330 newborns, were extracted from 986 studies. The weighted mean difference (WMD) of the platelet count was - 17.98 (p < 0.001), the platelet distribution width (PDW) was 0.27 (p = 0.266), the mean platelet volume (MPV) was 0.01 (p = 0.958), the plateletcrit (PCT) was - 0.03 (p < 0.001), and the platelet mass was - 150.10 (p = 0.001).Conclusion: Platelet count, PCT, and platelet mass of the first 3 days of life are potentially helpful in identifying premature infants at risk of hsPDA. More prospective studies on the relationship between different degrees of thrombocytopenia and platelet function and hsPDA should be conducted. What is Known: • Platelets are involved in the formation of thrombi during closure of the arterial duct. • The diagnosis of hsPDA by Doppler echocardiography and clinical signs is not precise enough. What is New: • Preterm newborns with hsPDA in the first week of life demonstrated a significant reduction in platelet count, platelet mass, and plateletcrit in the first 3 days of life. • No significant difference was shown between hsPDA and non-hsPDA infants in platelet distribution width and mean platelet volume in the first 3 days of life.
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http://dx.doi.org/10.1007/s00431-020-03802-5DOI Listing
March 2021

Subchondral bone cysts regress after correction of malalignment in knee osteoarthritis: comply with Wolff's law.

Int Orthop 2021 Feb 17;45(2):445-451. Epub 2020 Sep 17.

Department of Orthopaedic Surgery, University Paris East (UPEC), Hôpital Henri Mondor, Creteil, France.

Purpose: The purpose of this study is to discuss the mechanical function of subchondral bone cysts and its relationship with Wolff's law.

Methods: One hundred forty symptomatic knees (120 patients) with osteoarthritis were subjected to MRI before high tibial osteotomy (HTO). Subchondral bone cysts (SBCs) were detected on 72 knees of these 140 knees. SBCs, bone marrow lesion (BML), and hip-knee-ankle (HKA) axis were measured by using validated methods. After HTO, the evolution of cysts was evaluated on MRI performed with a five year follow-up on the 72 knees with pre-operative cysts.

Results: At baseline, 70 (97%) of these 72 knees had a BML surrounding the SBCs; the maximum cyst volume was 874 mm and the average cyst volume 9. 6 ± 4.1 mm. In the subregions where cysts were present, adjacent cartilage was still present without full thickness defects. The mean pre-operative hip-knee-ankle angle was 7.3 ± 3 degrees (0° to 14°) of varus and differed significantly (p = 0.01) between the 68 knees without cysts (average 3 ± 2 degrees) and the 72 knees with cysts (average 9.2 ± 4 degrees). Five years after HTO, the number of cysts had decreased, the maximum cyst volume was 532 mm, and the average cyst volume was 6.3 ± 2.8 mm.

Conclusion: Regression of subchondral bone cysts may be related to restoration of an appropriate load at the subchondral bone. With applying poroelasticity to bone mechaincs, this finding may suggest that SBCs and BMLs may be a physiological adaptation to mechanic overload. More basic research is needed to prove this matter.
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http://dx.doi.org/10.1007/s00264-020-04809-1DOI Listing
February 2021

Photo-augmented PHB production from CO or fructose by Cupriavidus necator and shape-optimized CdS nanorods.

Sci Total Environ 2021 Jan 31;753:142050. Epub 2020 Aug 31.

State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070, PR China; School of Chemistry, Chemical Engineering, and Life Science, Wuhan University of Technology, Wuhan 430070, PR China; School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, PR China. Electronic address:

Particulate photocatalysts developed for the solar energy-driven reduction of the greenhouse gas CO have a small product range and low specificity. Hybrid photosynthesis expands the number of products with photocatalysts harvesting sunlight and transferring charges to microbes harboring versatile metabolisms for bioproduction. Besides CO, abiotic photocatalysts have been employed to increase microbial production yields of reduced compounds from organic carbon substrates. Most single-reactor hybrid photosynthesis systems comprise CdS assembled in situ by microbial activity. This approach limits optimization of the morphology, crystal structure, and crystallinity of CdS for higher performance, which is usually done via synthesis methods incompatible with life. Here, shape and activity optimized CdS nanorods were hydrothermally produced and subsequently applied to Cupriavidus necator for the heterotrophic and autotrophic production of the bioplastic polyhydroxybutyrate (PHB). C. necator with CdS NR under light produced 1.5 times more PHB when compared to the same bacterium with suboptimal commercially-available CdS. Illuminated C. necator with CdS NR synthesized 1.41 g PHB from fructose over 120 h and 28 mg PHB from CO over 48 h. Interestingly, the beneficial effect of CdS NR was specific to C. necator as the metabolism of other microbes often employed for bioproduction including yeast and bacteria was negatively impacted. These results demonstrate that hybrid photosynthesis is more productive when the photocatalyst characteristics are optimized via a separated synthesis process prior to being coupled with microbes. Furthermore, bioproduction improvement by CdS-based photocatalyst requires specific microbial species highlighting the importance of screening efforts for the development of performant hybrid photosynthesis.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142050DOI Listing
January 2021

Single-neuron representation of learned complex sounds in the auditory cortex.

Nat Commun 2020 08 31;11(1):4361. Epub 2020 Aug 31.

Brain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, 400038, China.

The sensory responses of cortical neuronal populations following training have been extensively studied. However, the spike firing properties of individual cortical neurons following training remain unknown. Here, we have combined two-photon Ca imaging and single-cell electrophysiology in awake behaving mice following auditory associative training. We find a sparse set (~5%) of layer 2/3 neurons in the primary auditory cortex, each of which reliably exhibits high-rate prolonged burst firing responses to the trained sound. Such bursts are largely absent in the auditory cortex of untrained mice. Strikingly, in mice trained with different multitone chords, we discover distinct subsets of neurons that exhibit bursting responses specifically to a chord but neither to any constituent tone nor to the other chord. Thus, our results demonstrate an integrated representation of learned complex sounds in a small subset of cortical neurons.
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http://dx.doi.org/10.1038/s41467-020-18142-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459331PMC
August 2020

Common Gene Modules Identified for Chicken Adiposity by Network Construction and Comparison.

Front Genet 2020 29;11:537. Epub 2020 May 29.

College of Animal Science, Yangtze University, Jingzhou, China.

Excessive fat deposition can cause chicken health problem, and affect production efficiency by causing great economic losses to the industry. However, the molecular underpinnings of the complex adiposity trait remain elusive. In the current study, we constructed and compared the gene co-expression networks on four transcriptome profiling datasets, from two chicken lines under divergent selection for abdominal fat contents, in an attempt to dissect network compositions underlying adipose tissue growth and development. After functional enrichment analysis, nine network modules important to adipogenesis were discovered to be involved in lipid metabolism, PPAR and insulin signaling pathways, and contained hub genes related to adipogenesis, cell cycle, inflammation, and protein synthesis. Moreover, after additional functional annotation and network module comparisons, common sub-modules of similar functionality for chicken fat deposition were identified for different chicken lines, apart from modules specific to each chicken line. We further validated the lysosome pathway, and found and its downstream target genes showed similar expression patterns along with chicken preadipocyte differentiation. Our findings could provide novel insights into the genetic basis of complex adiposity traits, as well as human obesity and related metabolic diseases.
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http://dx.doi.org/10.3389/fgene.2020.00537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272656PMC
May 2020

MicroRNA-532-5p is implicated in the regulation of osteoporosis by forkhead box O1 and osteoblast differentiation.

BMC Musculoskelet Disord 2020 May 13;21(1):296. Epub 2020 May 13.

Orthopedic surgery of Wuhan General Hospital of People's Liberation Army, No.627 Wuluo Road, Wuhan City, Hubei Province, 430000, PR China.

Background: MicroRNAs (miRNAs) are critical regulators in osteogenesis and cartilage formation. This study was designed to investigate whether miR-532-5p plays a role in the regulation of osteoporosis.

Methods: Osteoporotic fractures (OP group, n = 10) or osteoarthritis without osteoporosis (control group, n = 10) were selected as subjects in this study. Quantitative analysis of gene expression was performed by RT-PCR. Western blot was used to determine the expression levels of protein forkhead O1 (FOXO1). Bioinformatics analyses and luciferase reporter assay were used to verify the downstream target of miR-532-5p.

Results: Compared with the non-osteoporotic controls, miR-532-5p was upregulated in osteoporotic samples, and expression of miR-532-5p was downregulated in the osteogenic C2C12 cell model. Overexpression of miR-532-5p resulted in decreased expression levels of key osteoblast markers, including alkaline phosphatase (ALP), osteocalcin (OC), and collagen type I alpha 1 (COL1A1). The inhibitory results of miR-532-5p were reversed. MiR-532-5p contained a putative FOXO1 binding site. Moreover, miR-532-5p inhibited the expression of FOXO1, and overexpression of FOXO1 inhibited the effect of miR-532-5p on osteoblast markers.

Conclusions: MiR-532-5p can provide references to osteoporosis by regulating the expression of FOXO1 and osteoblast differentiation. MiR-532-5p might serve as a therapeutic target for osteoporosis.
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http://dx.doi.org/10.1186/s12891-020-03317-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218624PMC
May 2020

The hidden chemolithoautotrophic metabolism of Geobacter sulfurreducens uncovered by adaptation to formate.

ISME J 2020 08 12;14(8):2078-2089. Epub 2020 May 12.

State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan, 430070, PR China.

Multiple Fe(III)-reducing Geobacter species including the model Geobacter sulfurreducens are thought to be incapable of carbon dioxide fixation. The discovery of the reversed oxidative tricarboxylic acid cycle (roTCA) for CO reduction with citrate synthase as key enzyme raises the possibility that G. sulfurreducens harbors the metabolic potential for chemolithoautotrophic growth. We investigate this hypothesis by transferring G. sulfurreducens PCA serially with Fe(III) as electron acceptor and formate as electron donor and carbon source. The evolved strain T17-3 grew chemolithoautotrophically with a 2.7-fold population increase over 48 h and a Fe(III) reduction rate of 417.5 μM h. T17-3 also grew with CO as carbon source. Mutations in T17-3 and enzymatic assays point to an adaptation process where the succinyl-CoA synthetase, which is inactive in the wild-type, became active to complete the roTCA cycle. Deletion of the genes coding for the succinyl-CoA synthetase in T17-3 prevented growth with formate as substrate. Enzymatic assays also showed that the citrate synthase can perform the necessary cleavage of citrate for the functional roTCA cycle. These results demonstrate that G. sulfurreducens after adaptation reduced CO via the roTCA cycle. This previously hidden metabolism can be harnessed for biotechnological applications and suggests hidden ecological functions for Geobacter.
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http://dx.doi.org/10.1038/s41396-020-0673-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368069PMC
August 2020

Effect of cell-extracellular matrix interaction on myogenic characteristics and artificial skeletal muscle tissue.

J Biosci Bioeng 2020 Jul 8;130(1):98-105. Epub 2020 Apr 8.

Graduate School of Human and Environmental, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8507, Japan; Division of Biological Chemistry, Radioisotope Research Center, Kyoto University, Yoshida-Konoe-Cho, Sakyo-ku, Kyoto 606-8507, Japan.

Although various types of artificial skeletal muscle tissue have been reported, the contractile forces generated by tissue-engineered artificial skeletal muscles remain to be improved for biological model and clinical applications. In this study, we investigated the effects of extracellular matrix (ECM) and supplementation of a small molecule, which has been reported to enhance α7β1 integrin expression (SU9516), on cell migration speed, cell fusion rate, myoblast (mouse C2C12 cells) differentiation and contractile force generation of tissue-engineered artificial skeletal muscles. When cells were cultured on varying ECM coated-surfaces, we observed significant enhancement in the migration speed, while the myotube formation (differentiation ratio) decreased in all except for cells cultured on Matrigel coated-surfaces. In contrast, SU9516 supplementation resulted in an increase in both the myotube width and differentiation ratio. Following combined culture with a Matrigel-coated surface and SU9516 supplementation, myotube width was further increased. Additionally, contractile forces produced by the tissue-engineered artificial skeletal muscles was augmented following combined culture. These findings indicate that regulation of the cell-ECM interaction is a promising approach to improve the function of tissue-engineered artificial skeletal muscles.
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http://dx.doi.org/10.1016/j.jbiosc.2020.02.008DOI Listing
July 2020

Chemical boundary engineering: A new route toward lean, ultrastrong yet ductile steels.

Sci Adv 2020 Mar 27;6(13):eaay1430. Epub 2020 Mar 27.

Key Laboratory for Advanced Materials of Ministry of Education, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

For decades, grain boundary engineering has proven to be one of the most effective approaches for tailoring the mechanical properties of metallic materials, although there are limits to the fineness and types of microstructures achievable, due to the rapid increase in grain size once being exposed to thermal loads (low thermal stability of crystallographic boundaries). Here, we deploy a unique chemical boundary engineering (CBE) approach, augmenting the variety in available alloy design strategies, which enables us to create a material with an ultrafine hierarchically heterogeneous microstructure even after heating to high temperatures. When applied to plain steels with carbon content of only up to 0.2 weight %, this approach yields ultimate strength levels beyond 2.0 GPa in combination with good ductility (>20%). Although demonstrated here for plain carbon steels, the CBE design approach is, in principle, applicable also to other alloys.
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http://dx.doi.org/10.1126/sciadv.aay1430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101205PMC
March 2020

Effect of preparation and reaction conditions on the performance of In/H-Beta for selective catalytic reduction of NO with CH.

Chemosphere 2020 Aug 11;252:126458. Epub 2020 Mar 11.

Shenzhen Key Laboratory of Organic Pollution Prevention and Control, Environmental Science and Engineering Research Center, Harbin Institute of Technology (Shenzhen), Shenzhen, 518055, PR China. Electronic address:

In/H-Beta catalyst was prepared by optimizing the support, concentration of ion exchange liquid and calcination temperature to investigate the effects of synthesis conditions on catalytic activity of selective catalytic reduction of NO with CH. The results showed that the In/H-Beta catalyst exhibited the superior activity when concentration of exchange liquid was 0.033 M and calcination temperature was 500 °C, the NO removal ratio could reach 97.6%. In addition, reaction conditions could affect the catalytic performance. When O concentration was 10%, CH:NO ratio was no less than one, space velocity was lower than 23600 h and NO initial concentration was no more than 700 ppm, In/H-Beta could exhibit superior catalytic activity. Moreover, the catalytic performances of In/H-Beta catalysts were discussed after enduring HO or/and SO. This novel strategy could open the door for selective catalytic reduction of NO with CH.
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http://dx.doi.org/10.1016/j.chemosphere.2020.126458DOI Listing
August 2020

A General Wet Transferring Approach for Diffusion-Facilitated Space-Confined Grown Perovskite Single-Crystalline Optoelectronic Thin Films.

Nano Lett 2020 Apr 19;20(4):2747-2755. Epub 2020 Mar 19.

Department of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, P.R. China.

Hybrid perovskite single-crystalline thin films are promising for making high-performance perovskite optoelectronic devices due to their superior physical properties. However, it is still challenging to incorporate them into multilayer devices because of their on-substrate growth. Here, a wet transfer method is used in transferring perovskite single-crystalline films perfectly onto various target substrates. More importantly, large millimeter-scaled single-crystalline films can be obtained via a diffusion-facilitated space-confined growth method as thin as a few hundred nanometers, which are capable of sustaining excellent crystalline quality and morphology after the transferring process. The availability of these crystalline films offers us a convenient route to further investigate their intrinsic properties of hybrid perovskites. We also demonstrate that the wet transfer method can be used for scalable fabrication of perovskite single-crystalline film-based photodetectors exhibiting a remarkable photoresponsivity. It is expected that this transferring strategy would promise broad applications of perovskite single-crystalline films for more complex perovskite devices.
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http://dx.doi.org/10.1021/acs.nanolett.0c00379DOI Listing
April 2020

Predisposition to Alzheimer's and Age-Related Brain Pathologies by PM2.5 Exposure: Perspective on the Roles of Oxidative Stress and TRPM2 Channel.

Front Physiol 2020 26;11:155. Epub 2020 Feb 26.

Sino-UK Joint Laboratory of Brain Function and Injury and Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, China.

Accumulating epidemiological evidence supports that chronic exposure to ambient fine particular matters of <2.5 μm (PM2.5) predisposes both children and adults to Alzheimer's disease (AD) and age-related brain damage leading to dementia. There is also experimental evidence to show that PM2.5 exposure results in early onset of AD-related pathologies in transgenic AD mice and development of AD-related and age-related brain pathologies in healthy rodents. Studies have also documented that PM2.5 exposure causes AD-linked molecular and cellular alterations, such as mitochondrial dysfunction, synaptic deficits, impaired neurite growth, neuronal cell death, glial cell activation, neuroinflammation, and neurovascular dysfunction, in addition to elevated levels of amyloid β (Aβ) and tau phosphorylation. Oxidative stress and the oxidative stress-sensitive TRPM2 channel play important roles in mediating multiple molecular and cellular alterations that underpin AD-related cognitive dysfunction. Documented evidence suggests critical engagement of oxidative stress and TRPM2 channel activation in various PM2.5-induced cellular effects. Here we discuss recent studies that favor causative relationships of PM2.5 exposure to increased AD prevalence and AD- and age-related pathologies, and raise the perspective on the roles of oxidative stress and the TRPM2 channel in mediating PM2.5-induced predisposition to AD and age-related brain damage.
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http://dx.doi.org/10.3389/fphys.2020.00155DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054442PMC
February 2020

Transient ischemia-reperfusion induces cortical hyperactivity and AMPAR trafficking in the somatosensory cortex.

Aging (Albany NY) 2020 03 9;12(5):4299-4321. Epub 2020 Mar 9.

School of Biomedical Engineering, Tianjin Medical University, Tianjin, China.

Brain ischemia results from cardiac arrest, stroke or head trauma. The structural basis of rescuing the synaptic impairment and cortical dysfunctions induced in the stage of ischemic-reperfusion can occur if therapeutic interventions are applied in time, but the functional basis for this resilience remains elusive. Here, we explore the changes in cortical activity and a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR) GluA1 subunit in spine (sGluA1) after transient ischemia-reperfusion for 28 days. Using two-photon microscopy in the mouse somatosensory cortex, we found that the average frequency of Ca transients in the spine (there was an unusual synchrony) was higher after 15 min of ischemia-reperfusion. In addition, the transient ischemia-reperfusion caused a reflective enhancement of AMPARs, which eventually restored to normal. The cortical hyperactivity (Ca transients) and the increase in AMPARs were successfully blocked by an NMDA receptor antagonist. Thus, the increase of AMPARs, cortical hyperactivity and the unusual synchrony might be the reason for reperfusion injury after short-term transient ischemia.
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http://dx.doi.org/10.18632/aging.102881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7093173PMC
March 2020

Characteristics of refractory organics in industrial wastewater treated using a Fenton-coagulation process.

Environ Technol 2020 Feb 28:1-9. Epub 2020 Feb 28.

State Key Lab of Environmental Aquatic Chem, Research Center for Eco-Environ. Sci., Chinese Academy of Sciences, Beijing, People's Republic of China.

It is a challenging environmental issue to develop a cost-efficient approach for the removal of low-concentration refractory organics in industrial wastewater. In this study, the Fenton-coagulation process was utilised to remove the organics from the industrial effluent. The operational conditions of the Fenton-coagulation process were optimised, and then, the molecular weight (MW) and resin fraction distribution of dissolved organic matter (DOM) were investigated before and after the Fenton-coagulation process. The results showed that the efficiency of organic matter removal was affected by the Fe/HO molar ratio, pH, and reaction time. The removal rate of chemical oxygen demand (COD) by Fenton-coagulation process reached 37.8% under the following conditions: pH = 4.0 - 5.0, HO concentration = 34 mg/L, Fe/HO molar ratio = 1.5, and reaction time = 120 min. The resin fraction distribution results showed that hydrophobic bases (HoB) were almost completely removed, and the removal rate of hydrophobic acids (HoA) reached 58%, while hydrophilic matter (HiM) became the dominant form in the final effluent after the Fenton-coagulation process due to the appearance of hydrophilic charged fractions (HiC). The results were explained by a two-step mechanism (Fenton oxidation and Fe coagulation). According to the molecular weight (MW), 35.7% removal of the main fractions of organic matter with MW < 1 kDa was achieved. Furthermore, a pilot test proved that the final effluent quality after the Fenton-coagulation process conformed to the first class of the A discharge standard of pollutants for municipal wastewater treatment plants in Tianjin.
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http://dx.doi.org/10.1080/09593330.2020.1732476DOI Listing
February 2020

Coordinated Actions Between p97 and Cullin-RING Ubiquitin Ligases for Protein Degradation.

Adv Exp Med Biol 2020 ;1217:61-78

Life Science Institute, Zhejiang University, HangZhou, China.

The cullin-RING ubiquitin ligases comprise the largest subfamily of ubiquitin ligases. They control ubiquitylation and degradation of a large number of protein substrates in eukaryotes. p97 is an ATPase domain-containing protein segregase. It plays essential roles in post-ubiquitylational events in the ubiquitin-proteasome pathway. Together with its cofactors, p97 collaborates with ubiquitin ligases to extract ubiquitylated substrates and deliver them to the proteasome for proteolysis. Here we review the structure, functions, and mechanisms of p97 in cellular protein degradation in coordination with its cofactors and the cullin-RING ubiquitin ligases.
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http://dx.doi.org/10.1007/978-981-15-1025-0_5DOI Listing
February 2020

A GSH Fluorescent Probe with a Large Stokes Shift and Its Application in Living Cells.

Sensors (Basel) 2019 Dec 4;19(24). Epub 2019 Dec 4.

Institute of Biomedical and Health Science, Anhui Science and Technology University, Bengbu 233030, China.

Intracellular GSH is the most abundant non-protein biothiol and acts as a central antioxidant to defend against aging toxins and radicals. Meanwhile abnormal level of intracellular GSH concentration is directly related to some diseases. In this case, detecting intracellular GSH rapidly and sensitively is of great significance. We synthesize a simple fluorescent probe (named GP) which can discriminate GSH from Cys (cysteine) or Hcy (homocysteine) and presents a 50-fold fluorescence increasing. The response time of to GSH was only 5 min and the product (the product of GP after reacting with GSH) after reacting with GSH possesses a larger Stokes shift for 135 nm than that in reported work. Probe can detect intracellular effectively and shows obvious yellow fluorescence. Briefly, probe can detect intracellular GSH rapidly and effectively both in vitro and in living cells.
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http://dx.doi.org/10.3390/s19245348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6960701PMC
December 2019

A disulfiram-loaded electrospun poly(vinylidene fluoride) nanofibrous scaffold for cancer treatment.

Nanotechnology 2020 Mar 25;31(11):115101. Epub 2019 Nov 25.

International Research Centre for Nano Handing and Manufacturing of China (CNM), Changchun University of Science and Technology, Changchun 130022, People's Republic of China.

Disulfiram (DSF), an FDA approved drug for the treatment of alcoholism, has shown its effectiveness against diverse cancer types. Thus, we developed a disulfiram-loaded scaffold using the electrospinning method to enhance the stability of DSF and to facilitate its appropriate distribution to tumor tissues. The drug release profile of the disulfiram-loaded scaffold was examined by high-performance liquid chromatography. We obtained mechanical and morphological characterizations of A549 cells treated with different scaffolds by various techniques to evaluate its antitumor properties. This work revealed that the cells after the treatment with the disulfiram-loaded scaffold exhibited a lower height and a larger elastic modulus compared with the untreated cells and those treated with the neat electrospun fibers. The changes were the indicators of cell apoptosis. Taken collectively, the results indicate that DSF was successfully incorporated into the electrospun fibers, and the disulfiram-loaded scaffold has great potential for inhibiting the regional recurrence of cancer.
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http://dx.doi.org/10.1088/1361-6528/ab5b35DOI Listing
March 2020

Cortical Plasticity Induced by Anodal Transcranial Pulsed Current Stimulation Investigated by Combining Two-Photon Imaging and Electrophysiological Recording.

Front Cell Neurosci 2019 29;13:400. Epub 2019 Aug 29.

Laboratory of Neurobiology, School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin, China.

Anodal-transcranial pulsed current stimulation (a-tPCS) has been used in human studies to modulate cortical excitability or improve behavioral performance in recent years. Multiple studies show crucial roles of astrocytes in cortical plasticity. The calcium activity in astrocytes could regulate synaptic transmission and synaptic plasticity. Whether the astrocytic activity is involved in a-tPCS-induced cortical plasticity is presently unknown. The purpose of this study is to investigate the calcium responses in neurons and astrocytes evoked by a-tPCS with different current intensities, and thereby provides some indication of the mechanisms underlying a-tPCS-induced cortical plasticity. Two-photon calcium imaging was used to record the calcium responses of neurons and astrocytes in mouse somatosensory cortex. Local field potential (LFP) evoked by sensory stimulation was used to assess the effects of a-tPCS on plasticity. We found that long-duration a-tPCS with high-intensity current could evoke large-amplitude calcium responses in both neurons and astrocytes, whereas long-duration a-tPCS with low-intensity current evoked large-amplitude calcium responses only in astrocytes. The astrocytic Ca elevations are driven by noradrenergic-dependent activation of the alpha-1 adrenergic receptors (A1ARs), while the intense Ca responses of neurons are driven by action potentials. LFP recordings demonstrated that low-intensity a-tPCS led to enhancement of cortical excitability while high-intensity a-tPCS resulted in diminution of cortical excitability. The results provide some evidence that the enhancement of a-tPCS-induced cortical excitability might be partly associated with calcium elevation in astrocytes, whereas the diminution of a-tPCS-induced cortical excitability might be caused by excessive calcium activity in neurons. These findings indicate that the appropriate current intensity should be used in the application of a-tPCS.
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http://dx.doi.org/10.3389/fncel.2019.00400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727068PMC
August 2019

Recent advances in synthesis and biosensors of two-dimensional MoS.

Nanotechnology 2019 Dec 10;30(50):502004. Epub 2019 Sep 10.

International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, People's Republic of China.

Two-dimensional (2D) transition metal dichalcogenides (TMDCs) have attracted tremendous research interests due to their exciting optical properties, large surface area, intercalatable morphologies and excellent electrochemically catalytic activity. Acting as the most typical member in TMDCs family, layer-dependent molybdenum disulfide (MoS) with particular direct bandgap of 1.8 eV in monolayer has been widely applied in various biosensors with high sensitivity and selectivity. In this review, the preparation methods of MoS, together with MoS-based biosensors for detecting cells and biomolecules (such as glucose, DNA and antigens) would be summarized. In addition, the current challenges and future perspectives are outlined for the applications of biosensors based on 2D MoS.
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http://dx.doi.org/10.1088/1361-6528/ab42feDOI Listing
December 2019

Electron transfer and intersystem crossing triggered fluorescence quenching detection of mercury ions.

Phys Chem Chem Phys 2019 Aug 18;21(30):16676-16685. Epub 2019 Jul 18.

College of Chemical and Materials Engineering, Anhui Science and Technology University, Fengyang, 233100, China.

Via a specific fluorescence quenching response, organic compound L serves as an excellent probe for Hg in aqueous solution. The underlying detection mechanism is proposed based on the analyses of orbital interactions between probe L and Hg, which is new and intrinsically different from the previously proposed one. By investigating the excitation process and the excited state deactivation process of the organometallic compound formed by L and Hg, two non-emissive channels, namely intermolecular electron transfer and intersystem crossing, are observed. The co-effect of the two channels leads to the significant fluorescence quenching of L. Hopefully, the newly proposed mechanism can inspire experimentalists to encapsulate the two channels into one probe to achieve accurate detection of metal ions.
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http://dx.doi.org/10.1039/c9cp02879jDOI Listing
August 2019

Reconsider the fluorescence properties of 5-ASA and its sensing mechanism for iodide ion.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Dec 8;223:117369. Epub 2019 Jul 8.

College of Chemical and Materials Engineering, Anhui Science and Technology University, Fengyang 233100, China.

5-ASA shows multiple fluorescence peaks at 408 nm, 480 nm and 500 nm in methanol solution, which can serve as ratiometric sensor for I. However, fluorescence mechanism of 5-ASA remains controversial and I detecting mechanism is unclear. With the aid of density functional theory and time-dependent density functional theory, this paper has carried out detailed investigations on both aspects at the molecular level. The origins of all the fluorescence peaks of 5-ASA have been assigned and a new fluorescence mechanism is proposed, which is convincing and intrinsically different from previously proposed mechanisms. Based on the new fluorescence mechanism, the I detecting mechanism is fully addressed. Two I detecting mechanisms have been proposed and are likely to coexist which lead to the sensitive detection of I.
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http://dx.doi.org/10.1016/j.saa.2019.117369DOI Listing
December 2019

Extended Fenton's process: toward improving biodegradability of drilling wastewater.

Water Sci Technol 2019 May;79(9):1790-1797

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China E-mail: University of Chinese Academy of Sciences, Beijing, 100049, China.

In this study, an extended Fenton process was used to improve biodegradability of the waste drilling mud containing bio-refractory polymers. Variation of biodegradability and organics with different molecular weights with the oxidation time were investigated during the Fenton oxidation process. Although the residual total organic carbon (TOC) arrived at a stable level soon after oxidation reaction, organics with the lower molecular weight increased and its biodegradability was improved significantly in the extended oxidation process, which originated from decomposition of residual HO catalyzed by transformation of the Fe/Fe and organoradicals. Under the conditions that follow: pH 3.0, HO 500 mg L, Fe 250 mg L, oxidation time 120 min, further TOC removal of 35.9% and biochemical oxygen demand and total organic carbon (BOD/TOC) ratio of 0.83 was achieved. At the biological test, a substantial increase in TOC degradation by biological treatment with extension of Fenton oxidation time was observed. Finally, more than 90% biological removal of the TOC was achieved for the 120 min oxidation treatment. The experimental results highlight that an extended process can be adopted to improve the biodegradability of wastewater by utilization of the slow reaction of hydrogen peroxide with Fe and organoradicals.
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http://dx.doi.org/10.2166/wst.2019.179DOI Listing
May 2019

Unconscious versus conscious thought in creative science problem finding: Unconscious thought showed no advantage!

Conscious Cogn 2019 05 16;71:109-113. Epub 2019 Apr 16.

Department of Psychology, Education and Child Studies, Erasmus University, Rotterdam, the Netherlands.

Unconscious thought theory (UTT) suggests that creativity benefits more from unconscious thought than conscious thought. However, previous studies have only focused on creative problem solving. This study aims to explore the effect of unconscious thought and conscious thought in creative science problem finding (CSPF). The ability of CSPF was measured by fluency, flexibility and originality. Participants accomplished the CSPF task after 3 min of distraction, during which unconscious thought was supposed to take place, or after 3 min of conscious thought. Results showed that unconscious thought had no advantage over conscious thought on CSPF. For the CSPF task with open instructions, conscious thought was comparable to unconscious thought in fluency, flexibility and originality. What's more, for the CSPF task with closed instructions, unconscious thought was even overtaken by conscious thought in fluency, flexibility and originality. These findings extend the unconscious thought theory and provide practical guidance on how to propose a creative science problem.
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http://dx.doi.org/10.1016/j.concog.2019.03.010DOI Listing
May 2019

Large eddy simulation of turbulent flow structure in a rectangular embayment zone with different population densities of vegetation.

Environ Sci Pollut Res Int 2019 May 15;26(14):14583-14597. Epub 2019 Mar 15.

State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan, 430072, China.

Dead-water zones (DWZs) in natural open channels, formed by embayment or consecutive groins, can provide favorable growth conditions for aquatic organisms. Although flow hydrodynamics in side-cavity zones have been well studied, the impact of vegetation on recirculating flow is rarely considered. This study adopts large eddy simulation (LES) to examine the flow field in a rectangular embayment zone with different population densities of vegetation. The numerical model is validated by mean streamwise velocity data collected near mid-depth in the physical experiment. Vegetation rearranges the circulation structure in the DWZ and weakens the velocity and turbulent kinetic energy. This negative effect increases with increasing population density. With the development of the shedding vortex induced in the front edge of the channel-embayment interface, the large-scale coherent structure forms in the mixing layer and is hardly affected by the variation of population density. As the population density increases, the mean retention time first decreases and then increases as a result of the combined action of three factors, namely, the large-scale coherent structure, the plant-induced Karman vortex street, and the blocking effect of dense vegetation.
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http://dx.doi.org/10.1007/s11356-019-04709-xDOI Listing
May 2019