Publications by authors named "Rahul Dubey"

11 Publications

  • Page 1 of 1

Molecular typing of canine parvovirus strains circulating from 2008 to 2012 in an organized kennel in India reveals the possibility of vaccination failure.

Infect Genet Evol 2014 Apr 30;23:1-6. Epub 2014 Jan 30.

Dte Gen Remount Veterinary Services, QMG's Branch, IHQ of MoD (Army), New Delhi, India.

Canine parvovirus-2 (CPV-2), which emerged in 1978, is considered as the major viral enteric pathogen of the canine population. With the emergence of new antigenic variants and incidences of vaccine failure, CPV has become one of the dreaded diseases of the canines worldwide. The present study was undertaken in an organized kennel from North India to ascertain the molecular basis of the CPV outbreaks in the vaccinated dogs. 415 samples were collected over a 5year period (2008-2012). The outbreak of the disease was more severe in 2012 with high incidence of mortality in pups with pronounced clinical symptoms. Molecular typing based on the VP2 gene was carried out with the 11 isolates from different years and compared with the CPV prototype and the vaccine strains. All the isolates in the study were either new CPV-2a (2012 isolates) or new CPV-2b (2008 and 2011 isolates). There were amino acid mutations at the Tyr324Ile and at the Thr440Ala position in five isolates from 2012 indicating new CPV mutants spreading in India. The CPV vaccines used in the present study failed to generate protective antibody titer against heterogeneous CPV antigenic types. The findings were confirmed when the affected pups were treated with hyper-immune heterogeneous purified immunoglobulin's against CPV in dogs of different antigenic types.
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April 2014

Assessment of adequate removal of ophthalmic viscoelastic device with irrigation/aspiration by quantifying intraocular lens 'Judders'.

Clin Exp Ophthalmol 2013 Jul;41(5):450-4

The University of New South Wales, Sydney, Australia.

Background: To assess the efficacy of 'Judders' as a technique reflecting adequacy of removal of ophthalmic viscoelastic device in cataract surgery.

Design: Prospective, consecutive, single surgeon study.

Participants: Cohort of 223 patients undergoing phacoemulsification.

Methods: 'Judders' are periodic, abrupt, horizontal displacements of the intraocular lens causing balanced salt solution to displace retropseudophakic ophthalmic viscoelastic device. The number of 'Judders' was correlated with axial length, anterior chamber depth, and preoperative and postoperative intraocular pressure.

Main Outcome Measures: Number of 'Judders', axial length, anterior chamber depth, day 1 postoperative intraocular pressure.

Results: The mean number of 'Judders' was 3.2. There was a positive association between the number of 'Judders' and axial length, but not between number of 'Judders' and anterior chamber depth. Mean preoperative intraocular pressure was 14.5 mmHg; mean day 1 postoperative intraocular pressure was 15.6 mmHg. Intraocular pressure rose in 47% of cases. In six cases (5%), intraocular pressure rose greater than 10 mmHg (range 11-23 mmHg) from the preoperative level.

Conclusions: Aspirating ophthalmic viscoelastic device with the irrigation/aspiration tip posterior to the intraocular lens may be associated with the risk of a posterior capsule tear. Maintaining the irrigation/aspiration tip anterior to the intraocular lens may offer a significant safety advantage. The number of 'Judders', usually 3-4, appears to be a safe and reliable end-point of complete ophthalmic viscoelastic device removal. There were significantly more 'Judders' in eyes with a longer axial length. The safety and efficacy of 'Judders' are reflected by the stable mean postoperative intraocular pressure.
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July 2013

Improved refractive outcome for ciliary sulcus-implanted intraocular lenses.

Ophthalmology 2012 Feb 23;119(2):261-5. Epub 2011 Dec 23.

The University of Sydney, Department of Ophthalmology, Sydney Eye Hospital, Save Sight Institute, 8 Macquarie Street, Sydney, NSW 2000 Australia.

Objective: To investigate the ideal correction of intraocular lens (IOL) power for sulcus implantation.

Design: Retrospective, comparative case series.

Participants: The records of 679 patients undergoing cataract surgery from June 2007 to June 2008 were reviewed.

Intervention: Eyes in this series underwent phacoemulsification and IOL implantation with local anesthesia. Patients in our study population had their IOL power reduced by 0.5 or 1 diopter (D) from that calculated by the SRK-T formula for in-the-bag implantation. The IOL implanted was the foldable 3-piece acrylic Acrysof MA60AC (Alcon Laboratories Inc., Fort Worth, TX).

Main Outcome Measures: In each case, the difference between actual spherical equivalent (SE) refraction and that predicted by biometry using the SRK-T formula was calculated.

Results: Posterior capsule tears requiring implantation of IOL in the ciliary sulcus occurred in 36 eyes. When comparing eyes in which the power was reduced by 0.5 D with those in which the reduction was 1.0 D, those with a power reduction of 1.0 D had significantly less unexpected error (0.49 vs. 1.01 D SE). After stratifying eyes by axial length (AL), we found higher unexpected refractive error in short eyes (<22 mm AL). Likewise, eyes with a predicted IOL power >25 D had a greater postoperative refractive error.

Conclusions: This is the first comparative clinical review examining adjustment of power of the sulcus-implanted IOL. We found that the IOL power should be adjusted according to the measured AL and predicted IOL power. For patients with a predicted IOL power from 18 to 25 D, power should be reduced by at least 1 D; for lenses >25 D, power should be reduced by 1.5 to 2 D.
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February 2012