Publications by authors named "Rafat Murad"

4 Publications

  • Page 1 of 1

Micro RNA: an early diagnostic marker for osteoporosis in post-menopausal women.

J Pak Med Assoc 2019 09;69(9):1406-1407

Department of Biological and Biomedical Sciences, Aga Khan University, Hospital, Karachi.

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September 2019

Comparison of serum levels of vitamin D and vitamin D-binding protein in normal, osteopenic and osteoporotic postmenopausal women.

Pak J Med Sci 2019 Mar-Apr;35(2):543-548

Rozeena Baig, MSc. Department of Biological & Biomedical Sciences, Aga Khan University, Karachi, Pakistan.

Objective: To compare the serum levels of vitamin D, vitamin D binding protein (VDBP) calcium and phosphate in normal, osteopenic and osteoporotic postmenopausal women categorized on the basis of bone mineral density (BMD) scores.

Methods: A cross sectional study carried out from May 2017 to August 2018. BMD measured by Dual energy X-ray Absorptiometry categorized women (aged 20- 70 years) into normal (n=37) (T score ≥ -1.0) osteopenic (n=25) (-2.5< T score, < -1) and osteoporotic (n= 26) (T score < -2.5) according to WHO classification. Serum concentrations of vitamin D, VDBP, calcium, phosphate analyzed by enzyme linked immunosorbent assay were compared by Analysis of Variance.

Results: In normal females higher levels of vitamin D and VDBP were observed [15.82 (8 - 69.18), 469.9 (269.57 - 875.55)] vs. osteopenic [(7.45 (4.66 - 15.1), 296.05 (232.58 - 420.23)] and osteoporotic women [(7.25 (3.97 - 17.49), 272.94 (202.23 - 351.24)]; [median interquartile range]; p value < 0.0001.

Conclusion: Vitamin D and VDBP are linked with bone health and estimation of VDBP appears to be a valuable tool for the assessment of increased bone loss and possible risks of bone fractures especially in postmenopausal women.
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http://dx.doi.org/10.12669/pjms.35.2.714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500817PMC
May 2019

Serum tumour necrosis factor alpha in osteopenic and osteoporotic postmenopausal females: A cross-sectional study in Pakistan.

J Pak Med Assoc 2018 Mar;68(3):428-431

Aga Khan University.

Objective: To compare biochemical parameters serum tumour necrosis factor alpha, calcium, magnesium, bone-specific alkaline phosphatase and vitamin D in postmenopausal women.

Methods: This cross-sectional study was carried out from June 2015 to July 2016 at Jinnah Medical and Dental College, Karachi, and comprised postmenopausal women. Bone mineral density done by dual energy X-ray absorptiometryscan categorised subjects by World Health Organisation classification into normal (T score > -1) osteopenic (T score between -1 and -2.5) and osteoporotic (T score < -2.5). Biochemical parameters like tumour necrosis alpha, calcium, magnesium, bone-specific alkaline phosphatase and vitamin D were measured by solid phase enzyme amplified sensitivity immunoassay method. SPSS 16 was used to analyse the data.

Results: Of the 146 women, 34(23%) were normal, 93(67%) were osteopenic and 19(13%) were osteoporotic. There was significant difference in mean body mass index, serum tumour necrosis factor alpha and calcium in all the three groups (p<0.01). Significant mean difference was observed in serum calcium levels between normal and osteopenic, and between normal and osteoporotic group (p<0.05 each) without any significant mean difference between osteopenic and osteoporotic groups (p>0.05). A significant difference was observed for mean tumour necrosis factor alpha values between normal and osteoporotic groups (p<0.05). Tumour necrosis factor alpha showed negative correlation with bone mineral density in osteopenic and osteoporotic groups (p>0.05).

Conclusions: Increased bone turnover in postmenopausal osteopenic women can be predicted by increased serum cytokine.
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March 2018

Frequency of tetanus toxoid immunization among college/university female students of Karachi.

J Ayub Med Coll Abbottabad 2010 Jan-Mar;22(1):147-9

Department of Community Health Sciences, Hamdard College of Medicine and Dentistry, Karachi, Pakistan.

Background: Tetanus is a deadly infectious disease for which immunisation is available in EPI at both infant level and for females of reproductive age. More than 95% of patients who develop tetanus have not been previously immunised. Objectives of the study were to determine the frequency of tetanus vaccination and to access the awareness of immunisation among females studying in 11 girls' colleges of Karachi and University of Karachi.

Method: A cross sectional study was conducted among 1,407 females studying in colleges and University of Karachi from April to August 2007 using a prescribed questionnaire.

Result: Among 1,407 female students who were interviewed for the study, 232 (16.48%) were not aware about tetanus immunisation program for females of reproductive age. Only 560 students (39.80%) received at least 1 of 5 recommended doses. Only 41 female students (2.91%) received complete course of 5 doses.

Conclusion: Coverage of tetanus immunisation among literate females in most populous city of the country is far behind satisfactory. There is need for awareness and crash programs of tetanus immunisation.
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April 2011
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