Arch Med Sci 2019 Mar 31;15(2):504-512. Epub 2017 Jul 31.
Department of Genetics, Chair of Molecular Medicine, Faculty of Medicine, University of Rzeszow, Rzeszow, Poland.
Introduction: and alterations play a crucial role in glioblastoma (GB) pathogenesis. and function is affected by several pathologic mechanisms, such as point mutations or promoter methylation, which are well characterized. Expression of both genes can be regulated by other mechanisms as well, e.g., microRNAs (miRNAs). Moreover, cross-talk among various pathologic processes may occur, further affecting and functionality.
Material And Methods: In 49 GB patients, we analyzed the possible associations between and its miRNA regulators , , and , as well as and its miRNA regulators and . We evaluated the possible influence of mutational and methylation status on the pre-identified associations.
Results: In patients with immunohistochemistry-detected overexpression, expression levels of and were negatively correlated ( = -0.56, = 0.0195), and in patients with mutations, expression levels of and were negatively correlated ( = -0.67, = 0.0330). In patients with methylation, expression levels of were negatively correlated with and expression levels ( = -0.61, = 0.0269 and = -0.34, = 0.0727, respectively).
Conclusions: Our findings demonstrate that selected miRNAs are significantly correlated with and levels, but the extent of this correlation differs regarding the and mutational and promoter methylation status.