Publications by authors named "R Zinovieva"

24 Publications

[Molecular-genetic mechanisms of cornea morphogenesis].

Izv Akad Nauk Ser Biol 2015 Mar-Apr(2):117-26

In this paper, we analyzed our own results and published data on the expression of regulatory genes encoding transcription factors Pax6/PAX6, Pitx2/PITX2, Fox1/FOXC1, Prox1/PROX1, Oct4/OCT4, Nanog/NANOG, and TGFβ2 signaling protein during morphogenesis of the cornea in vertebrates. We considered the results obtained for the cornea of model animals, primarily mice, and human fetal cornea. The main possibility of establishing common mechanisms of eye development in vertebrates in health and disease is comparative studies of eye morphogenesis of humans and animal models.
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June 2015

Expression of nucleostemin in proliferating and differentiating cells of the human retina during prenatal development.

Dokl Biol Sci 2012 Jul-Aug;445:244-6. Epub 2012 Sep 4.

Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.

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http://dx.doi.org/10.1134/S0012496612040084DOI Listing
October 2012

Temporal and spatial distribution of PAX6 gene expression in the developing human eye.

Dokl Biol Sci 2009 May-Jun;426:264-6

Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.

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http://dx.doi.org/10.1134/s0012496609030211DOI Listing
September 2009

Cell phenotypes in human amniotic fluid.

Acta Naturae 2009 Jul;1(2):98-103

Koltzov Institute of Developmental Biology, Russian Academy of Sciences;

Stem cells capable of long-term proliferation and differentiation into different cell types may be a promising source of cells for regenerative medicine. Recently, much attention has been paid to fetal stem cells, among which are cells from amniotic fluid (AF). We have isolated amniotic stem cells from 3 AF samples. Flow cytometry, RT -PCR and immunohistochemistry have shown that these cells express mesenchymal (CD90, CD73, CD105, CD13, CD29, CD44, and CD146), neural (≤3-tubulin, Nestin, and Pax6), epithelial (keratin 19 and p63) markers and also markers of pluripotency (Oct4, Nanog, and Rex-1). Transplantation of the cells to nude mice does not lead to tumor formation. Thus, putative stem/progenitor cells from AF are capable of long-term proliferation in vitro and the profile of gene expression led us to speculate that they have greater differentiation potential than mesenchymal stem cells and may be useful for cell therapy.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347518PMC
July 2009

Differentiation of embryonic stem cells after transplantation into peritoneal cavity of irradiated mice and expression of specific germ cell genes in pluripotent cells.

Transplant Proc 2005 Jan-Feb;37(1):295-8

Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.

Permanent embryonic stem cell lines (ES cells) are considered as one of the most promising cellular sources for regenerative medicine. ES cells have a high proliferative potency and ability to differentiate into all kinds of somatic and germ cells. However, transplantation of undifferentiated ES cells into adult recipient tissue results in the formation of teratomas. To understand the mechanisms underlying self-renewal and determination of pluripotent cells, we investigated differentiation potencies of undifferentiated ES cells and differentiating embryoid bodies (EB). ES cells and EBs growing on acetate-cellulose membranes were transplanted into the peritoneal cavity of irradiated mice. Behavior and differentiation of transplanted cells were studied within 1, 2, 3, and 6 weeks after transplantation. No differences in the cell composition were found in the teratomas formed by ES cells and differentiating EBs. The pattern of expression of the genes specific for pluripotent and germ cells was studied in all types of experimental teratomas. The expression of oct4, stella, fragilis was detected in the teratomas, but nanog was not expressed. We conclude that pluripotent cells are retained in the experimental teratomas formed after transplantation of ES cells and EBs but the pattern of expression of the studied genes underwent changes.
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http://dx.doi.org/10.1016/j.transproceed.2004.12.184DOI Listing
October 2005
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