Publications by authors named "R Tang"

3,196 Publications

  • Page 1 of 1

Different Roles of Subcortical Inputs in V1 Responses to Luminance and Contrast.

Eur J Neurosci 2021 Apr 13. Epub 2021 Apr 13.

State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.

Cells in the primary visual cortex (V1) generally respond weakly to large uniform luminance stimuli. Only a subset of V1 cells is thought to encode uniform luminance information. In natural scenes, local luminance is an important feature for defining an object that varies and coexists with local spatial contrast. However, the strategies used by V1 cells to encode local mean luminance for spatial contrast stimuli remain largely unclear. Here, using extracellular recordings in anaesthetized cats, we investigated the responses of V1 cells by comparing with those of retinal ganglion (RG) cells and lateral geniculate nucleus (LGN) cells to simultaneous and rapid changes in luminance and spatial contrast. Almost all V1 cells exhibited a strong monotonic increasing luminance tuning when they were exposed to high spatial contrast. Thus, V1 cells encode the luminance carried by spatial contrast stimuli with the monotonically increasing response function. Moreover, high contrast decreased luminance tuning of OFF cells but increased that of in ON cells in RG and LGN. The luminance and contrast tunings of LGN ON cells were highly separable as V1 cells, whereas those of LGN OFF cells were lowly separable. These asymmetrical effects of spatial contrast on ON/OFF channels might underlie the robust ability of V1 cells to perform luminance tuning when exposed to spatial contrast stimuli.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/ejn.15233DOI Listing
April 2021

MicroRNA-30 regulates left ventricular hypertrophy in chronic kidney disease.

JCI Insight 2021 Apr 13. Epub 2021 Apr 13.

National Clinical Research Center for Kidney Diseases, Nanjing University School of Medicine, Nanjing, China.

Left ventricular hypertrophy (LVH) is a primary feature of cardiovascular complications in chronic kidney disease (CKD) patients. MiRNA-30 is an important posttranscriptional regulator of LVH, but it is unknown whether miRNA-30 participates in the process of CKD-induced LVH. In the present study, we found that CKD not only results in LVH but also suppresses miRNA-30 expression in the myocardium. Rescue of cardiomyocyte-specific miRNA-30 attenuates LVH in CKD rats without altering CKD progression. Importantly, in vivo and in vitro knockdown of miRNA-30 in cardiomyocytes leads to cardiomyocyte hypertrophy by upregulating the calcineurin signalling directly. Furthermore, CKD-related detrimental factors, such as fibroblast growth factor-23 (FGF-23), uraemic toxin, angiotensin-II (Ang-II) and transforming growth factor-β (TGF-β), suppress cardiac miRNA-30 expression, while miRNA-30 supplementation blunts cardiomyocyte hypertrophy induced by such factors. These results uncover a novel mechanism of CKD-induced LVH and provide a potential therapeutic target for CKD patients with LVH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1172/jci.insight.138027DOI Listing
April 2021

MicroRNA‑524‑5p regulates the proliferation and invasion of HTR‑8/SVneo trophoblasts by targeting NUMB in the Notch signaling pathway.

Mol Med Rep 2021 Jun 13;23(6). Epub 2021 Apr 13.

Department of Obstetrics, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, P.R. China.

Preeclampsia is a pregnancy disorder that is primarily associated with maternal and neonatal or fetal morbidity and mortality. The discovery of dysregulated microRNAs (miRs) and their roles in preeclampsia has provided new insight into the mechanisms involved in pregnancy‑related disorders. In the present study, quantitative PCR demonstrated that the expression levels of miR‑524‑5p were lower in patients with preeclampsia than those in normal pregnant women. Cell Counting Kit‑8 and Transwell assays indicated that overexpression of miR‑524‑5p promoted the proliferation and invasion of HTR‑8/SVneo cells, whereas inhibition of miR‑524‑5p suppressed HTR‑8/SVneo cell proliferation and invasion. Furthermore, NUMB endocytic adaptor protein (NUMB), a negative regulator of the Notch signaling pathway and a target gene of miR‑524‑5p, limited the effects of miR‑524‑5p on HTR‑8/SVneo cell invasion and migration. The present study demonstrated that miR‑524‑5p regulated the proliferation and invasion of HTR‑8/SVneo cells at least partly by targeting NUMB to regulate the Notch signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2021.12075DOI Listing
June 2021

Wnt5a/PRP synergistically inhibits IL-1β induced inflammatory activity through NF-κB signaling pathway and prevents cartilage damage and promotes meniscus regeneration.

J Tissue Eng Regen Med 2021 Apr 11. Epub 2021 Apr 11.

Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, P.R. China.

Objective: Non-canonical Wnt5a is a particularly attractive growth factor to maintain chondrogenesis. Platelet rich plasma (PRP) is an autologous blood-derived product and a source of bioactive growth factors involved in tissue regeneration. The present study aimed to investigate the effect and inflammation reaction of Wnt5a/PRP on meniscus cells, and evaluate meniscus regeneration and osteoarthritis prevention by the application of Wnt5a/PRP gel in a rabbit model of massive meniscal defect.

Design: In vitro, the proliferation, migration, differentiation and IL-1β induced inflammation reaction of meniscus cells treated by Wnt5a and PRP was assessed. In vivo, the anterior half of the medial meniscus of 18 New Zealand Rabbits was excised and implanted with PRP gel, Wnt5a/PRP gel or untreated. After 6 and 12 weeks, the regenerated meniscus were evaluated.

Results: Wnt5a can promote the migration of meniscus cells. PRP and Wnt5a had synergistic effect in promoting the proliferation and chondrogenic differentiation of meniscus cells. The IL-1β induced meniscus cells study showed that PRP and Wnt5a had the anti-inflammatory actions through NF-κB signaling pathway. PRP and Wnt5a/PRP significantly inhibited the increase of the p-p65/p65 and p-IκB-α/IκB-α ratios. In vivo transplantation of Wnt5a/PRP gel was demonstrated to promote meniscus regeneration, while reducing osteoarthritis of knee joint.

Conclusions: Wnt5a with PRP had the anti-inflammatory activity in an IL-1β-induced inflammatory model. They can synergistically improve the chondorgenic differentiation of meniscus cells. Wnt5a/PRP gel treatment could potentially be developed into a new method for meniscus regeneration and the prevention of osteoarthritis. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/term.3198DOI Listing
April 2021

A clinical diagnostic model based on an eXtreme Gradient Boosting algorithm to distinguish type 1 diabetes.

Ann Transl Med 2021 Mar;9(5):409

Department of Metabolism and Endocrinology, the Second Xiangya Hospital, Central South University, Changsha, China.

Background: Accurate classification of type 1 diabetes (T1DM) and type 2 diabetes (T2DM) in the early phase is crucial for individual precision treatment. This study aimed to develop a classification model having fewer and easier to access clinical variables to distinguish T1DM in newly diagnosed diabetes in adults.

Methods: Clinical and laboratory data were collected from 15,206 adults with newly diagnosed diabetes in this cross-sectional study. This cohort represented 20 provinces and 4 municipalities in China. Types of diabetes were determined based on postprandial C-peptide (PCP) level and glutamic acid decarboxylase autoantibody (GADA) titer. We developed multivariable clinical diagnostic models using the eXtreme Gradient Boosting (XGBoost) algorithm. Classification variables included in the final model were based on their scores of importance. Model performance was evaluated by area under the receiver operating characteristic curve (ROC AUC), sensitivity, and specificity. The performance of models with different variable combinations was compared. Calibration intercept and slope were evaluated for the final model.

Results: Among the newly diagnosed diabetes cohort, 1,465 (9.63%) persons had T1DM and 13,741 (90.37%) had T2DM. Body mass index (BMI) contributed the most to the model, followed by age of onset and hemoglobin A1c (HbA1c). Compared with models with other clinical variable combinations, a final model that integrated age of onset, BMI and HbA1c had relatively higher performance. The ROC AUC, sensitivity, and specificity for this model were 0.83 (95% CI, 0.80 to 0.85), 0.77, and 0.76, respectively. The calibration intercept and slope were 0.02 (95% CI, -0.03 to 0.06) and 0.90 (95% CI, 0.79 to 1.02), respectively, which suggested a good calibration performance.

Conclusions: Our classification model that integrated age of onset, BMI, and HbA1c could distinguish T1DM from T2DM, which provides a useful tool in assisting physicians in subtyping and precising treatment in diabetes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-7115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033361PMC
March 2021

Glomerular Immune Deposition in MPO-ANCA Associated Glomerulonephritis Is Associated With Poor Renal Survival.

Front Immunol 2021 25;12:625672. Epub 2021 Mar 25.

Department of Nephrology, Xiangya Hospital, Central South University, Changsha, China.

Background: Rapidly progressive glomerulonephritis caused by antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is typically characterized as pauci-immune glomerulonephritis. However, immune complex (IC) deposition in the glomerulus has been reported in a growing number of studies. Here, we assess the presence of glomerular immune deposits alongside renal outcome in myeloperoxidase (MPO)-ANCA associated glomerulonephritis (MPO-ANCA GN).

Methods: Clinical and histopathologic characteristics of 97 patients with MPO-ANCA GN classified by renal biopsy from January 2008 to December 2019 were extracted retrospectively from electronic medical records. The extent of immune deposits in the kidney (C3, C4, C1q, IgA, IgG, IgM) at diagnosis were analyzed by immunofluorescence (IF). Patients were followed up for a median period of 15 months. The response to treatment and outcomes of renal and histological lesion changes were also assessed.

Results: In our study, 41% (40/97) of patients showed positive IF (≥2+) for at least one of the six immunoglobulin or complement components tested. Patients with IC deposits showed higher levels of serum creatinine (p=0.025), lower platelet counts (p=0.009), lower serum complement C3 (sC3) (≤790 ml/L) (p=0.013) and serum IgG (p=0.018) than patients with pauci-immune (PI) deposition at diagnosis. End-stage renal disease was negatively associated with eGFR (HR 0.885, 95% CI 0.837 to 0.935, p<0.0001), platelet count (HR 0.996, 95% CI 0.992 to 1.000, p=0.046) and serum globulin (HR 0.905, 95% CI 0.854 to 0.959, p=0.001). Patients with lower sC3 levels showed a worse renal outcome than the patients with normal sC3 at diagnosis (p=0.003). Analysis of the components of the renal deposits found that patients with IgG deposits exhibited a poorer renal outcome compared to patients that were IgG negative (p=0.028). Moreover, Bowman's capsule rupture occurred less frequently in patients with IgM deposition compared with IgM negative counterparts (p=0.028). Vascular lesions and granuloma-like lesions had been seen more frequently in cases with IgA deposition than those without IgA deposition (p=0.03 and 0.015, respectively).

Conclusion: In conclusion, patients with immune complex deposits in the kidney showed less platelet count, lower sC3 and sIgG levels, and higher serum creatinine levels. Patients with low sC3 at initial and with continued low sC3 during the treatment displayed a trend toward poorer kidney survival. Moreover, the IC group showed a worse renal outcome than the PI group, further enforcing the present strategy of introducing complement targeted therapies in AAV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.625672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027492PMC
March 2021

Longitudinal imaging of T-cells and inflammatory demyelination in a preclinical model of multiple sclerosis using F-FAraG PET and MRI.

J Nucl Med 2021 Apr 9. Epub 2021 Apr 9.

University of California San Francisco, United States.

Lymphocytes and innate immune cells are key drivers of multiple sclerosis (MS) and are the main target of MS disease modifying therapies (DMT). Ex vivo analyses of MS lesions have revealed cellular heterogeneity and variable T-cell levels, which may have important implication for patient stratification and choice of DMT. Although magnetic resonance imaging (MRI) has proven valuable to monitor DMT efficacy, its lack of specificity for cellular subtypes highlights the need for complementary methods to improve lesion characterization. Here, we evaluated the potential of 2'-deoxy-2'-[F]fluoro-9-β-D-arabinofuranosylguanine (F-FAraG) PET imaging to non-invasively assess infiltrating T-cells and to provide, in combination with MRI, a novel tool to determine lesion types. We used a novel MS mouse model that combines cuprizone and experimental autoimmune encephalomyelitis (EAE) to reproducibly induce two brain inflammatory lesion types, differentiated by their T-cell content. F-FAraG PET imaging, T2-weighted MRI, and T1-weighted contrast-enhanced MRI were performed prior to disease induction, during demyelination with high levels of innate immune cells, and following T-cell infiltration. Fingolimod immunotherapy was used to evaluate the ability of PET and MRI to detect therapy response. Ex vivo immunofluorescence analyses for T-cells, microglia/macrophages, myelin and blood brain barrier (BBB) integrity were performed to validate the in vivo findings. F-FAraG signal was significantly increased in brain and spinal cord at the time point of T-cell infiltration. F-FAraG signal from white matter (corpus callosum), and grey matter (cortex, hippocampus) further correlated with T-cell density. T2-weighted MRI detected white matter lesions independently of T-cells. T1-weighted contrast-enhanced MRI indicated BBB disruption at the time point of T-cell infiltration. Fingolimod treatment prevented motor deficits and decreased T-cell and microglia/macrophage levels. In agreement, F-FAraG signal was decreased in brain and spinal cord of Fingolimod-treated mice, T1-weighted contrast-enhanced MRI revealed intact BBB, while T2-weighted MRI remained unchanged. The combination of MRI and F-FAraG PET enables detection of inflammatory demyelination and T-cell infiltration in a MS mouse model, providing a new way to evaluate lesion heterogeneity during disease progression and following DMT. Upon clinical translation, these methods hold great potential for patient stratification, monitoring MS progression and therapy responses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2967/jnumed.120.259325DOI Listing
April 2021

[Diagnosis and surgical treatment of sinonasal phosphaturic mesenchymal tumor].

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Apr;56(4):351-355

Department of Otorhinolaryngology Head and Neck Surgery, Shanghai JiaoTong University Affiliated Sixth People's Hospital, Shanghai 200233, China.

To investigate the diagnosis and surgical treatment of sinonasal phosphaturic mesenchymal tumor (PMT). The medical records of nine patients who had been diagnosed as sinonasal PMT in Department of Otorhinolaryngology Head and Neck Surgery, Shanghai JiaoTong University Affiliated Sixth People's Hospital between January 2015 and May 2020 were collected, including 4 males and 5 females, ranging from 36 to 59 years. The patient's previous history, clinical manifestations, imaging findings, laboratory results, surgical procedure, pathological results and postoperative follow-up data were analyzed by descriptive statistical analysis. All patients presented hypophosphatemia and tumor-induced osteomalacia (TIO) with a disease course of 1 to 19 years. The imaging examination and intraoperative findings identified two cases with peripheral tissue infiltration, two cases with contralateral nasal cavity invasion, and one case with intracranial invasion. Five patients underwent unilateral endoscopic resection while two patients underwent bilateral endoscopic resection, and the remaining two patients underwent unilateral transorbital ethmoid artery ligation plus endoscopic tumor resection and endoscopic combined with transfrontal tumor resection (=1 each). Expect for one case developed recurrence and intracranial involvement, the other patients achieved clinical remission and no recurrence was observed during the six-month follow-up. The diagnosis of sinonasal PMT needs combination of clinical manifestation, imaging, and pathological findings. Complete surgical excision and long-term postoperative follow-up are imperative.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3760/cma.j.cn115330-20200605-00477DOI Listing
April 2021

Finite-Time Synchronization of Markovian Coupled Neural Networks With Delays via Intermittent Quantized Control: Linear Programming Approach.

IEEE Trans Neural Netw Learn Syst 2021 Apr 8;PP. Epub 2021 Apr 8.

This article is devoted to investigating finite-time synchronization (FTS) for coupled neural networks (CNNs) with time-varying delays and Markovian jumping topologies by using an intermittent quantized controller. Due to the intermittent property, it is very hard to surmount the effects of time delays and ascertain the settling time. A new lemma with novel finite-time stability inequality is developed first. Then, by constructing a new Lyapunov functional and utilizing linear programming (LP) method, several sufficient conditions are obtained to assure that the Markovian CNNs achieve synchronization with an isolated node in a settling time that relies on the initial values of considered systems, the width of control and rest intervals, and the time delays. The control gains are designed by solving the LP. Moreover, an optimal algorithm is given to enhance the accuracy in estimating the settling time. Finally, a numerical example is provided to show the merits and correctness of the theoretical analysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNNLS.2021.3069926DOI Listing
April 2021

Role of tumor mutation burden-related signatures in the prognosis and immune microenvironment of pancreatic ductal adenocarcinoma.

Cancer Cell Int 2021 Apr 7;21(1):196. Epub 2021 Apr 7.

Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, No. 270 Dong'An Road, Shanghai, 200032, China.

Background: High tumor mutation burden (TMB) has gradually become a sensitive biomarker for predicting the response to immunotherapy in many cancers, including lung, bladder and head and neck cancers. However, whether high TMB predicts the response to immunotherapy and prognosis in pancreatic ductal adenocarcinoma (PDAC) remained obscure. Hence, it is significant to investigate the role of genes related to TMB (TRGs) in PDAC.

Methods: The transcriptome and mutation data of PDAC was downloaded from The Cancer Genome Atlas-Pancreatic Adenocarcinoma (TCGA). Five independent external datasets of PDAC were chosen to validate parts of our results. qRT-PCR and immunohistochemical staining were also performed to promote the reliability of this study.

Results: The median overall survival (OS) was significantly increased in TMB_low group compared with the counterpart with higher TMB score after tumor purity adjusted (P = 0.03). 718 differentially expressed TRGs were identified and functionally enriched in some oncogenic pathways. 67 TRGs were associated with OS in PDAC. A prognostic model for the OS was constructed and showed a high predictive accuracy (AUC = 0.849). We also found TMB score was associated with multiple immune components and signatures in tumor microenvironment. In addition, we identified a PDAC subgroup featured with TMBMicrosatellite instability (MSI) was associated with prolonged OS and a key molecule, ANKRD55, potentially mediating the survival benefits.

Conclusion: This study analyzed the biological function, prognosis value, implications for mutation landscape and potential influence on immune microenvironment of TRGs in PDAC, which contributed to get aware of the role of TMB in PDAC. Future studies are expected to investigate how these TRGs regulate the initiation, development or repression of PDAC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12935-021-01900-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028808PMC
April 2021

De novo full length transcriptome analysis of Arachis glabrata provides insights into gene expression dynamics in response to biotic and abiotic stresses.

Genomics 2021 Apr 2;113(3):1579-1588. Epub 2021 Apr 2.

Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, People's Republic of China; College of Life Sciences, Shandong Normal University, Jinan 250014, People's Republic of China. Electronic address:

The perennial ornamental peanut Arachis glabrata represents one of the most adaptable wild Arachis species. This study used PacBio combined with BGISEQ-500 RNA-seq technology to study the transcriptome and gene expression dynamics of A. glabrata. Of the total 109,747 unique transcripts obtained, >90,566 transcripts showed significant homology to known proteins and contained the complete coding sequence (CDS). RNA-seq revealed that 1229, 1039, 1671, 3923, 1521 and 1799 transcripts expressed specifically in the root, stem, leaf, flower, peg and pod, respectively. We also identified thousands of differentially expressed transcripts in response to drought, salt, cold and leaf spot disease. Furthermore, we identified 30 polyphenol oxidase encoding genes associated with the quality of forage, making A. glabrata suitable as a forage crop. Our findings presented the first transcriptome study of A. glabrata which will facilitate genetic and genomics studies and lays the groundwork for a deeper understanding of the A. glabrata genome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ygeno.2021.03.030DOI Listing
April 2021

Augmented Reality-Assisted Pancreaticoduodenectomy with Superior Mesenteric Vein Resection and Reconstruction.

Gastroenterol Res Pract 2021 17;2021:9621323. Epub 2021 Mar 17.

Hepatopancreatobiliary Center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Institute for Precision Medicine, Tsinghua University, Beijing 102218, China.

Introduction: Pancreaticoduodenectomy (PD) with superior mesenteric vein (SMV) reconstruction are often required to achieve complete (R0) resection for pancreatic head cancer (PHC) with tumor invasion of the SMV. Augmented reality (AR) technology can be used to assist in determining the extent of SMV involvement by superimposing virtual 3-dimensional (3D) images of the pancreas and regional vasculature on the surgical field.

Materials And Methods: Three patients with PHC and tumor invasion of the SMV underwent AR-assisted PD with SMV resection and reconstruction following preoperative computed tomography scanning. Preoperative imaging data were used to reconstruct 3D images of anatomical structures, including the tumor, portal vein (PV), SMV, and splenic vein (SV). Using AR software installed on a smart phone, the reconstructed 3D images were superimposed on the surgical field as viewed in a smart phone display to provide intermittent navigational assistance to the surgeon in identifying the boundaries of PHC tumor invasion for resection of the vessels involved.

Result: All patients successfully completed the operation. Intraoperative AR applications displayed virtual images of the pancreas, SMV, bile duct, common hepatic artery (CHA), and superior mesenteric artery (SMA). Two patients required end-to-end anastomosis for reconstruction of the SMV. One patient required allogenic vascular bypass to reconstruct the SMV-PV juncture with concomitant reconstruction of the SV-SMV confluence by end-to-side anastomosis of the SV and bypass vessel. Postoperative pathology confirmed R0 resections for all patients.

Conclusion: AR navigation technology based on preoperative CT image data can assist surgeons performing PD with SMV resection and reconstruction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/9621323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990556PMC
March 2021

High Blood Eosinophil and YKL-40 Levels, as Well as Low CXCL9 Levels, are Associated with Increased Readmission in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease.

Int J Chron Obstruct Pulmon Dis 2021 26;16:795-806. Epub 2021 Mar 26.

Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.

Background: Readmission after hospital discharge is common among patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Predictive biomarkers of readmission would facilitate stratification strategies and individualized prognosis. Therefore, this study aimed to investigate the utility of type 2 biomarkers (eosinophils, periostin, and YKL-40) and a type 1 biomarker (CXCL9) in predicting readmission events in patients with AECOPD.

Methods: This is a prospective observational study design. Blood levels of eosinophils, periostin, YKL-40, and CXCL9 were measured at admission. The clinical outcomes were 12-month COPD-related readmission, time to COPD-related readmission, and number of 12-month COPD-related readmissions. These outcomes were analyzed using logistic and Cox regression models and Spearman's rank test.

Results: A total of 123 patients were included, of whom 51 had experienced at least one readmission for AECOPD. High levels of eosinophils (≥200 cells/μL or 2% of the total white blood cell count, adjusted odds ratio [aOR] =3.138, =0.009) and YKL-40 (≥14.5 ng/mL, aOR =2.840, =0.015), as well as low CXCL9 levels (≤30.1 ng/mL, aOR =2.551, =0.028), were associated with an increased COPD-related readmission. The highest relative readmission rate was observed in patients with both high eosinophil and YKL-40 levels. Moreover, high eosinophil and YKL-40 levels were associated with a shorter time to first COPD-related readmission and an increased number of 12-month COPD-related readmissions.

Conclusion: High blood eosinophil and YKL-40 levels, as well as low CXCL9 levels, have predictive utility for the 12-month COPD-related readmission rate. Using eosinophils and YKL-40 together allows more precise identification of patients at high risk of COPD-related readmission.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/COPD.S294968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009765PMC
March 2021

5-Substituted isatin thiosemicarbazones as inhibitors of tyrosinase: Insights of substituent effects.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Mar 9;255:119669. Epub 2021 Mar 9.

College of Chemistry and Environmental Engineering, Institute of Environmental Toxicology and Environmental Ecology, Yancheng Teachers University, Xiwang Avenue South Rd. 2, Yancheng 224007, PR China. Electronic address:

Seven isatin-thiosemicarbazone analogues bearing different substituents (R) attached at C-5 of the indoline ring, TSC-ISA-R (R = -H, -CH, -OCH, -OCF, -F, -Cl and -NO), were synthesized and evaluated as inhibitors of mushroom tyrosinase (TYR). The inhibitory behaviour and performance of TSC-ISA-R were investigated spectroscopically in relation to the substituent modifications through examining their inhibition against the diphenolase activity of TYR using L-DOPA as a substrate. The IC values of TSC-ISA-R were determined to be in the range of 81-209 μM. The kinetic analysis showed that TSC-ISA-R were reversible and mixed type inhibitors. Three potential non-covalent interactions rather than complexation including the binding of TSC-ISA-R with free TYR, TYR-L-DOPA complex, and with substrate L-DOPA were found to be involved in the inhibition. The substituent modifications affected these interactions by varying the characters of the resulting TSC-ISA-R in different degrees. The thiosemicarbazido moiety of each TSC-ISA-R contributed predominantly to the inhibition, and the isatin moiety seemed to play a regulatory role in the binding of TSC-ISA-R to the target molecules. The results of theoretical calculations using density functional theory method indicated a different effect of -R on the electron distribution in HOMO of TSC-ISA-R. The LUMO-HOMO energy gap of TSC-ISA-R almost accords with the trend of their experimental inhibition potency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2021.119669DOI Listing
March 2021

Rat Locomotion Detection Based on Brain Functional Directed Connectivity from Implanted Electroencephalography Signals.

Brain Sci 2021 Mar 9;11(3). Epub 2021 Mar 9.

School of Mechatronical Engineering, Beijing Institute of Technology, Beijing 100081, China.

Previous findings have suggested that the cortex involved in walking control in freely locomotion rats. Moreover, the spectral characteristics of cortical activity showed significant differences in different walking conditions. However, whether brain connectivity presents a significant difference during rats walking under different behavior conditions has yet to be verified. Similarly, whether brain connectivity can be used in locomotion detection remains unknown. To address those concerns, we recorded locomotion and implanted electroencephalography signals in freely moving rats performing two kinds of task conditions (upslope and downslope walking). The Granger causality method was used to determine brain functional directed connectivity in rats during these processes. Machine learning algorithms were then used to categorize the two walking states, based on functional directed connectivity. We found significant differences in brain functional directed connectivity varied between upslope and downslope walking. Moreover, locomotion detection based on brain connectivity achieved the highest accuracy (91.45%), sensitivity (90.93%), specificity (91.3%), and F1-score (91.43%). Specifically, the classification results indicated that connectivity features in the high gamma band contained the most discriminative information with respect to locomotion detection in rats, with the support vector machine classifier exhibiting the most efficient performance. Our study not only suggests that brain functional directed connectivity in rats showed significant differences in various behavioral contexts but also proposed a method for classifying the locomotion states of rat walking, based on brain functional directed connectivity. These findings elucidate the characteristics of neural information interaction between various cortical areas in freely walking rats.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/brainsci11030345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998315PMC
March 2021

Air Quality During COVID-19 Lockdown in the Yangtze River Delta and the Pearl River Delta: Two Different Responsive Mechanisms to Emission Reductions in China.

Environ Sci Technol 2021 Apr 2. Epub 2021 Apr 2.

Joint International Research Laboratory of Atmospheric and Earth System Sciences, School of Atmospheric Sciences, Nanjing University, Nanjing 210023, China.

Despite the large reduction in anthropogenic activities due to the outbreak of COVID-19, air quality in China has witnessed little improvement and featured great regional disparities. Here, by combining observational data and simulations, this work aims to understand the diverse air quality response in two city clusters, Yangtze River Delta region (YRD) and Pearl River Delta region (PRD), China. Though there was a noticeable drop in primary pollutants in both the regions, differently, the maximum daily 8 h average ozone (O) soared by 20.6-76.8% in YRD but decreased by 15.5-28.1% in PRD. In YRD, nitrogen oxide (NOx) reductions enhanced O accumulation and hence increased secondary aerosol formation. Such an increment in secondary organic and inorganic aerosols under stationary weather reached up to 36.4 and 10.2%, respectively, which was further intensified by regional transport. PRD was quite the opposite. The emission reductions benefited PRD air quality, while regional transport corresponded to an increase of 17.3 and 9.3% in secondary organic and inorganic aerosols, respectively. Apart from meteorology, the discrepancy in O-VOCs-NOx relationships determined the different O responses, indicating that future emission control shall be regionally specific, instead of one-size-fits-all cut. Overall, the importance of regionally coordinated and balanced control strategy for multiple pollutants is highly emphasized.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.0c08383DOI Listing
April 2021

lncRNA SNHG16 Mediates Cell Proliferation and Apoptosis in Cholangiocarcinoma by Directly Targeting miR-146a-5p/GATA6 Axis.

Biochem Genet 2021 Apr 2. Epub 2021 Apr 2.

Department of Hepatobiliary Surgery, Zhangjiajie People's Hospital, No.192 Guyong Road, Yongding District, Zhangjiajie, 427000, Hunan Province, People's Republic of China.

Cholangiocarcinoma (CCA) is a malignant tumour with high recurrence and mortality rates and poor prognosis. However, the pathogenic mechanism remains unclear. In the present study, we aimed to investigate the roles and regulatory mechanism of SNHG16 in the occurrence and development of CCA. Gene Expression Profiling Interactive Analysis (GEPIA) was used to predict the expressions of SNHG16 and GATA6 in CCA samples from TCGA database. The levels of SNHG16, miR-146a-5p and GATA6 were evaluated using qRT-PCR. CCK-8 and flow cytometry assays were conducted to evaluate cell proliferation and apoptosis, respectively. Western blotting was applied to analyse the protein levels of GATA6 and apoptosis-related proteins. SNHG16 was significantly elevated in CCA tissues from TCGA database and CCA cell lines. Moreover, downregulation of SNHG16 restricted cell proliferation and increased apoptotic rate of RBE and HuCCT1 cells. miR-146a-5p, a downstream target of SNHG16, was shown to be an intermediate mediator of GATA6 expression regulated by SNHG16. In addition, either the miR-146a-5p inhibitor or overexpression of GATA6 obviously impaired the regulatory effects of SNHG16 downregulation in RBE and HuCCT1 cells. These data demonstrated that SNHG16 promoted cell proliferation and repressed apoptosis by regulating the miR-146a-5p/GATA6 axis, which provides some helpful insights for the diagnosis and treatment of CCA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10528-021-10059-6DOI Listing
April 2021

Implications of gut microbiota in autoimmune liver diseases.

Minerva Gastroenterol (Torino) 2021 Apr 1. Epub 2021 Apr 1.

Division of Gastroenterology and Hepatology, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, State Key Laboratory for Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, Shanghai, China -

Autoimmune liver diseases (AILD) is a group of immune-mediated liver inflammatory diseases with three major forms including autoimmune hepatitis (AIH), primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). Interaction of both genetic and environmental factors leads to the breakdown of self-tolerance, hence resulting in hyper-responsive of autoantibodies and aggressive autoreactive immune cells. Genetic studies have identified dozens of risk loci associated with initiation and development of AILD. However, the role of exogenous factors remains unclear. Recently, both infectious and inflammatory diseases have been associated with microbiota, which colonizes multiple mucosal surfaces and participates in human physiological process and function in immune system, particularly influencing liver and biliary system via gut-liver axis. Emerging evidence on the role of gut microbiota has expanded our knowledge of AILD in both pathogenesis and potential therapeutic targets, along with putative diagnosis biomarkers. Herein we review the relationship between host and gut microbiota, discuss their potential roles in disease onset and progression, and summarize the compositional and functional alterations of the microbiota in AILD. We also highlight the microbiotabased therapeutics such as antibiotics and fecal microbiota transplantation (FMT).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S2724-5985.21.02860-9DOI Listing
April 2021

Study on the Reparative Effect of PEGylated Growth Hormone on Ovarian Parameters and Mitochondrial Function of Oocytes From Rats With Premature Ovarian Insufficiency.

Front Cell Dev Biol 2021 15;9:649005. Epub 2021 Mar 15.

Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Premature ovarian insufficiency (POI) is a heterogeneous disorder and lacks effective interventions in clinical applications. This research aimed to elucidate the potential effects of recombinant human PEGylated growth hormone (rhGH) on follicular development and mitochondrial function in oocytes as well as ovarian parameters in POI rats induced by the chemotherapeutic agent. The impacts of rhGH on ovarian function before superovulation on follicles, estrous cycle, and sex hormones were evaluated. Oocytes were retrieved to determine oocyte quality and oxidative stress parameters. Single-cell sequencing was applied to investigate the latent regulatory network. This study provides new evidence that a high dosage of rhGH increased the number of retrieved oocytes even though it did not completely restore the disturbed estrous cycle and sex hormones. rhGH attenuated the apoptosis of granulosa cells and oxidative stress response caused by reactive oxygen species (ROS) and mitochondrial superoxide. Additionally, rhGH modulated the energy metabolism of oocytes concerning the mitochondrial membrane potential and ATP content but not mtDNA copy numbers. Based on single-cell transcriptomic analysis, we found that rhGH directly or indirectly promoted the balance of oxidative stress and cellular oxidant detoxification. Four hub genes, Pxmp4, Ehbp1, Mt-cyb, and Enpp6, were identified to be closely related to the repair process in oocytes as potential targets for POI treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.649005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8005617PMC
March 2021

Different Chinese herbal medicine therapy for idiopathic thrombocytopenic purpura: A protocol for systematic review and Bayesian network meta-analysis.

Medicine (Baltimore) 2021 Apr;100(13):e25341

Department of Hematology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Xiuying District, Haikou City, Hainan Province, China.

Background: Idiopathic thrombocytopenic purpura (ITP) is a common immune system and blood system disease in clinical practice, and it is a hemorrhagic disease caused by immune factors causing platelet destruction and decreasing number of platelets. There is currently no effective treatment plan for ITP. At this stage, glucocorticoid and gamma globulin are mostly used in the treatment of ITP, and some patients use splenectomy to achieve therapeutic purposes, but the various treatment methods are inadequate. At this stage, a large number of randomized controlled studies have reported that Chinese herbal medicine has achieved good curative effect in the treatment of ITP. However, due to the variety of Chinese herbal medicine, there has been no evidence of the effectiveness and safety of Chinese herbal medicine in the treatment of ITP. Because of the above reasons, this study uses the network meta-analysis method based on Bayesian method to compare the clinical efficacy and safety of different kinds of Chinese herbal medicine in the treatment of ITP through direct and indirect comparison, in order to provide evidence-based medical support for the treatment of ITP with Chinese herbal medicine.

Methods: This study uses the method of combining free words with theme words, and using computer to retrieve PubMed, EMbase, The Cochrane Library, WANFANG Database, CNKI, and VIP Database, etc, to collect the randomized control studies on Chinese herbal medicine in the treatment of ITP. The retrieval time is from the establishment of each database to January 2021, and the retrieval languages are Chinese and English. Two researchers independently read the title, abstract and full text of the article to determine whether it is included in the literature; In the event of a disagreement, a third researcher will join the discussion to resolve the disagreement; For the literature that lacks information, trying to contact the original author of the document to supplement it. The literature quality evaluation carried out by using the Stata 14.0 software to draw network and funnel plots, in accordance with the quality evaluation criteria of version 5.1.0 of the Cochrane System Evaluation Manual. Statistical analysis is performed by using ADDIS 1.16.8 software based on the Bayesian model.

Results: This study will compare the clinical efficacy and safety of different types of Chinese herbal medicine in the treatment of idiopathic thrombocytopenic purpura through the method of network meta-analysis, and rank the different types of Chinese herbal medicine according to their effectiveness, and the results will be published in a peer-reviewed, high-quality academic journal.

Conclusion: This study will find effective and safe Chinese herbal medicine for clinical treatment of ITP from the perspective of evidence-based medicine, and benefit more ITP patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021348PMC
April 2021

Sanjie Yiliu Formula Inhibits Colorectal Cancer Growth by Suppression of Proliferation and Induction of Apoptosis.

ACS Omega 2021 Mar 9;6(11):7761-7770. Epub 2021 Mar 9.

Institute of Chemistry, University of Sargodha, Sargodha 40100, Pakistan.

Colorectal cancer (CRC) is one of the most common malignancies worldwide. As current therapies toward CRC, including chemotherapy and radiotherapy, pose limitations, such as multidrug resistance (MDR) as well as the intrinsic and potential cytotoxic effects, necessitating to find more effective treatment options with fewer side effects, traditional Chinese medicine (TCM) has an advantage in complementary therapies. In the present study, 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assays), trypan blue staining, colony formation, 4,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining, cell cycle determination, and Annexin V-FITC/PI staining were used to examine the efficacy of Sanjie Yiliu Formula (SJYLF) against CRC proliferation and to investigate its underlying molecular mechanisms through protein expression of various proapoptotic factors by quantitative polymerase chain reaction (q-PCR) and Western blotting. This four-herb-TCM SJYLF can be suggested as one of the decoctions clinically effective in late-stage cancer treatment. Our results suggest that SJYLF robustly decreased the viability of only CRC cell lines (HCT-8, SW-480, HT-29, and DLD-1) and not the normal human kidney cells (HK-2). Moreover, SJYLF significantly suppressed proliferation and induced apoptosis in HCT-8 and downregulated cyclin D1, CDK4, and BCL-2, while Bax expression was upregulated at both mRNA and protein expression levels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.0c05565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992181PMC
March 2021

Deciphering the Prognostic Implications of the Components and Signatures in the Immune Microenvironment of Pancreatic Ductal Adenocarcinoma.

Front Immunol 2021 10;12:648917. Epub 2021 Mar 10.

Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.

The treatment modalities for pancreatic ductal adenocarcinoma (PDAC) are limited and unsatisfactory. Although many novel drugs targeting the tumor microenvironment, such as immune checkpoint inhibitors, have shown promising efficacy for some tumors, few of them significantly prolong the survival of patients with PDAC due to insufficient knowledge on the tumor microenvironment. A single-cell RNA sequencing (scRNA-seq) dataset and seven PDAC cohorts with complete clinical and bulk sequencing data were collected for bioinformatics analysis. The relative proportions of each cell type were estimated using the gene set variation analysis (GSVA) algorithm based on the signatures identified by scRNA-seq or previous literature. A meta-analysis of 883 PDAC patients showed that neutrophils are associated with worse overall survival (OS) for PDAC, while CD8+ T cells, CD4+ T cells, and B cells are related to prolonged OS for PDAC, with marginal statistical significance. Seventeen cell categories were identified by clustering analysis based on single-cell sequencing. Among them, CD8+ T cells and NKT cells were universally exhausted by expressing exhaustion-associated molecular markers. Interestingly, signatures of CD8+ T cells and NKT cells predicted prolonged OS for PDAC only in the presence of "targets" for pyroptosis and ferroptosis induction. Moreover, a specific state of T cells with overexpression of ribosome-related proteins was associated with a good prognosis. In addition, the hematopoietic stem cell (HSC)-like signature predicted prolonged OS in PDAC. Weighted gene co-expression network analysis identified 5 hub genes whose downregulation may mediate the observed survival benefits of the HSC-like signature. Moreover, trajectory analysis revealed that myeloid cells evolutionarily consisted of 7 states, and antigen-presenting molecules and complement-associated genes were lost along the pseudotime flow. Consensus clustering based on the differentially expressed genes between two states harboring the longest pseudotime span identified two PDAC groups with prognostic differences, and more infiltrated immune cells and activated immune signatures may account for the survival benefits. This study systematically investigated the prognostic implications of the components of the PDAC tumor microenvironment by integrating single-cell sequencing and bulk sequencing, and future studies are expected to develop novel targeted agents for PDAC treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.648917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987951PMC
March 2021

Gene Expression Differences Between Developmental Stages of the Fall Armyworm ().

DNA Cell Biol 2021 Apr 23;40(4):580-588. Epub 2021 Mar 23.

Key Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, China.

The fall armyworm () is one of the most significant agricultural pests in the world and invaded China in early 2019. We sampled and sequenced RNA-seq data from 15 individuals across different developmental stages. Developmental stages were the larval stage (5th instar larvae and 6th instar larvae), chrysalis stage, and adult stage (female adult and male adult). Individual samples were mainly clustered by developmental stages and we then identified variation between developmental stages of differentially expressed transcripts (DETs). There were 2136 upregulated DETs and 1391 downregulated DETs in the larval stage when comparing larval and chrysalis stages. In the comparison between the chrysalis and adult stages, there were 2033 upregulated DETs and 1391 downregulated DETs in the chrysalis stage. In total, 19,195 abundantly expressed transcripts were obtained and 10% of them were DETs. We then obtained stage-specific DETs to investigate the potential function of the fall armyworm during different developmental stages. We also constructed our annotation background set for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. This indicated that the fall armyworm may undergo active metabolism during its lifespan, even in the chrysalis stage. And it also may experience detoxifying and xenobiotic metabolism throughout its life, especially in the larval stage, which partially explains the difficulty to eradicate using chemical control. Our study is the first insight into the developmental patterns of the fall armyworm and we also provide the fundamental information about enhanced drug resistance at the level of transcriptome. These results are beneficial for a future investigation related to the eradication and/or control stage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/dna.2020.6191DOI Listing
April 2021

Smoothing inertial neurodynamic approach for sparse signal reconstruction via L-norm minimization.

Neural Netw 2021 Feb 27;140:100-112. Epub 2021 Feb 27.

Key Laboratory of Dependable Services Computing in Cyber-Physical Society (Chongqing) Ministry of Education, College of Computer Science, Chongqing University, Chongqing, 400044, China.

In this paper, we propose a smoothing inertial neurodynamic approach (SINA) which is used to deal with L-norm minimization problem to reconstruct sparse signals. Note that the considered optimization problem is nonsmooth, nonconvex and non-Lipschitz. First, the problem is transformed into a smooth optimization problem based on smoothing approximation method, and the Lipschitz property of gradient of the smooth objective function is discussed. Then, SINA based on Karush-Kuhn-Tucker (KKT) condition, smoothing approximation and inertial dynamical approach, is designed to handle smooth optimization problem. The existence, uniqueness, global convergence and optimality of the solution of the SINA are discussed by the Cauchy-Lipschitz-Picard theorem, energy function and KKT condition. In addition, for p=1, the SINA has a mean sublinear convergence rate O1∕t under some mild conditions. Finally, some numerical examples on sparse signal reconstruction and image restoration are given to illustrate the theoretical results and the efficiency of SINA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neunet.2021.02.006DOI Listing
February 2021

Spore Polysaccharide Inhibits the Growth of Hepatocellular Carcinoma Cells by Altering Macrophage Polarity and Induction of Apoptosis.

J Immunol Res 2021 5;2021:6696606. Epub 2021 Mar 5.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.

Background: has certain components with known pharmacological effects, including strengthening immunity and anti-inflammatory activity. seeds inherit all its biological characteristics. spore polysaccharide (GLSP) is the main active ingredient to enhance these effects. However, its specific biological mechanisms are not exact. Our research is aimed at revealing the specific biological mechanism of GLSP to enhance immunity and inhibit the growth of H22 hepatocellular carcinoma cells.

Methods: We extracted primary macrophages (M) from BALB/c mice and treated them with GLSP (800 g/mL, 400 g/mL, and 200 g/mL) to observe its effects on macrophage polarization and cytokine secretion. We used GLSP and GLSP-intervened macrophage supernatant to treat H22 tumor cells and observed their effects using MTT and flow cytometry. Moreover, real-time fluorescent quantitative PCR and western blotting were used to observe the effect of GLSP-intervened macrophage supernatant on the PI3K/AKT and mitochondrial apoptosis pathways.

Results: In this study, GLSP promoted the polarization of primary macrophages to M1 type and the upregulation of some cytokines such as TNF-, IL-1, IL-6, and TGF-1. The MTT assay revealed that GLSP+M at 400 g/mL and 800 g/mL significantly inhibited H22 cell proliferation in a dose-dependent manner. Flow cytometry analysis revealed that GLSP+Mø induced apoptosis and cell cycle arrest at the G2/M phase, associated with the expression of critical genes and proteins (PI3K, p-AKT, BCL-2, BAX, and caspase-9) that regulate the PI3K/AKT pathway and apoptosis. GLSP reshapes the tumor microenvironment by activating macrophages, promotes the polarization of primary macrophages to M1 type, and promotes the secretion of various inflammatory factors and cytokines.

Conclusion: Therefore, as a natural nutrient, GLSP is a potential agent in hepatocellular carcinoma cell treatment and induction of apoptosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6696606DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954632PMC
March 2021

Prevalence and Antifungal Susceptibility of Species Complex in Eastern China: A 15-Year Retrospective Study by ECIFIG.

Front Microbiol 2021 4;12:644000. Epub 2021 Mar 4.

Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

complex is one of the most common non- species that cause candidemia, especially invasive candidiasis. The purpose of this study was to evaluate the antifungal susceptibilities of both colonized and invasive clinical complex isolates to 10 drugs: amphotericin (AMB), anidulafungin (AFG), caspofungin (CAS), micafungin (MFG), fluconazole (FLZ), voriconazole (VRZ), itraconazole (ITZ), posaconazole (POZ), 5-flucytosine (FCY), and isaconazole (ISA). In total, 884 species complex isolates were gathered between January 2005 and December 2020. , , and accounted for 86.3, 8.1, and 5.5% of the cryptic species, respectively. The resistance/non-wild-type rate of bloodstream to the drugs was 3.5%, of to AFG and CAS was 7.7%, and of to FLZ and VRZ was 15% and to CAS, MFG, and POZ was 5%. The geometric mean (GM) minimum inhibitory concentrations (MICs) of non-bloodstream for CAS (0.555 mg/L), MFG (0.853 mg/L), FLZ (0.816 mg/L), VRZ (0.017 mg/L), ITZ (0.076 mg/L), and POZ (0.042 mg/L) were significantly higher than those of bloodstream , for which the GM MICs were 0.464, 0.745, 0.704, 0.015, 0.061, and 0.033 mg/L, respectively ( < 0.05). The MIC distribution of the bloodstream strains collected from 2019 to 2020 for VRZ, POZ, and ITZ were 0.018, 0.040, and 0.073 mg/L, significantly higher than those from 2005 to 2018, which were 0.013, 0.028, and 0.052 mg/L ( < 0.05). Additionally, MIC distributions of with FLZ and the distributions of with ITZ and POZ might be higher than those in Clinical and Laboratory Standards Institute studies. Furthermore, a total of 143 complex isolates showed great susceptibility to ISA. Overall, antifungal treatment of the non-bloodstream complex isolates should be managed and improved. The clinicians are suggested to pay more attention on azoles usage for the complex isolates. In addition, establishing the epidemiological cutoff values (ECVs) for azoles used in Eastern China may offer better guidance for clinical treatments. Although ISA acts on the same target as other azoles, it may be used as an alternative therapy for cases caused by FLZ- or VRZ-resistant complex strains.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.644000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969513PMC
March 2021

[Spectral Characteristics and Sources of Dissolved Organic Matter from Landscape River During Flood Season in Suzhou Based on EEMs and UV-vis].

Huan Jing Ke Xue 2021 Apr;42(4):1889-1900

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.

Based on excitation emission matrix spectroscopy (EEMs) technology combined with the parallel factor analysis (PARAFAC) and UV-vis spectra, the spectral characteristics and sources of dissolved organic matter (DOM) from a landscape river were analyzed during different periods of the flood season in Suzou. Four fluorescent components were identified using the PARAFAC model, including two humus-like components (C1, C4) and two protein-like components (C2, C3), with a significant correlation coefficient (<0.01) in C2 and C3/C4 and C3 and C4, respectively. During the early flood season, the total fluorescence intensity of the DOM in the river was relatively higher due to the influence of initial rainwater but reduced significantly towards the middle and late flood season. The fluorescence characteristic parameters indicated that the autochthonous contribution of DOM were substantial during the early stages of the flood season. On the contrary, there were increased levels of DOM largely from terrestrial origins during the middle flooding period. During the entire flood season, SUVA, SUVA, and E2/E3 exhibited the same trend, that is, decreasing first and then increasing. As a result of the continuous heavy rainfall during the flood season, the nitrogen and phosphorus nutrient content in the channel increased. The algae population did not proliferate in large quantities because of the strong hydrodynamic conditions experienced throughout the flood season. The fluorescence components C2, C3, and C4 exhibited a significant correlation coefficient (<0.01) with the characteristic parameters (FI, HIX, BIX, and :α). All the fluorescence components had a high correlation (<0.05) with DOC. There was a considerable correlation between fluorescence component C1 and Chla. The principal component analysis revealed that the DOM components in the landscape river during different periods of the flood season exhibited notable differences, and the continuous heavy rainfall during the flood season has a substantial influence on the content of C2, C3, and C4 components in the water body.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13227/j.hjkx.202009087DOI Listing
April 2021

Incidence and predictors of hospitalization in patients with atrial fibrillation: results from the Chinese atrial fibrillation registry study.

BMC Cardiovasc Disord 2021 Mar 19;21(1):146. Epub 2021 Mar 19.

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, No. 2 Beijing Anzhen Road, Chaoyang District, Beijing, 100029, People's Republic of China.

Background: Patients with atrial fibrillation (AF) underwent a high risk of hospitalization, which has not been paid much attention to in practice. Therefore, we aimed to assess the incidence, causes and predictors of hospitalization in AF patients.

Methods: From August 2011 to December 2017, a total number of 20,172 AF patients from the Chinese Atrial Fibrillation Registry (China-AF) Study were prospectively selected for this study. We described the incidence, causes of hospitalization by age groups and sex. The Fine-Gray competing risk model was employed to identify predictors of first all-cause and first cause-specific hospitalization.

Results: After a mean follow-up of 37.3 ± 20.4 months, 7,512 (37.2%) AF patients experienced one or more hospitalizations. The overall incidence of all-cause hospitalization was 24.0 per 100 patient-years. Patients aged < 65 years were predominantly hospitalized for AF (42.1% of the total hospitalizations); while patients aged 65-74 and ≥ 75 years were mainly hospitalized for non-cardiovascular diseases (43.6% and 49.3%, respectively). We found patients complicated with heart failure (HF)[hazard ratio (HR) 1.10, 95% confidence interval (CI) 1.02-1.18], established coronary artery disease (CAD) (HR 1.24, 95%CI 1.17-1.33), ischemic stroke/transient ischemic attack (TIA) (HR 1.22, 95%CI 1.15-1.30), diabetes (HR 1.14, 95%CI 1.08-1.20), chronic obstructive pulmonary disease (COPD) (HR 1.28, 95%CI 1.02-1.62), gastrointestinal disorder (HR 1.37, 95%CI 1.21-1.55), and renal dysfunction (HR 1.24, 95%CI 1.09-1.42) had higher risks of hospitalization.

Conclusions: More than one-third of AF patients included in this study were hospitalized at least once during over 3-year follow-up. The main cause for hospitalization among the elderly patients (≥ 65 years) is non-cardiovascular diseases rather than AF. Multidisciplinary management of comorbidities should be advocated to reduce hospitalization in AF patients older than 65 years old. Clinical Registry http://www.chictr.org.cn/showproj.aspx?proj=5831 . Unique identifier: ChiCTR-OCH-13003729. The registration date is October 22, 2013.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12872-021-01951-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7980549PMC
March 2021