Publications by authors named "R Prasad"

2,205 Publications

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Predictors of patient and graft survival following pediatric liver transplantation: Long-term analysis of more than 300 cases from single centre.

Pediatr Transplant 2021 Sep 20:e14139. Epub 2021 Sep 20.

The Leeds Teaching Hospitals, NHS Foundation Trust, Leeds, UK.

Background: Pediatric liver transplant (PLT) activity has flourished over time although with limited expansion in the graft pool. The study aims to identify pre-transplant factors that predict post-transplant patient and graft survival in the PLT population.

Methods: Retrospective review of PLTs at a single tertiary transplant unit from 2000 to 2019. Univariate and multivariate analyses of pre-transplant factors were performed to identify predictors of patient and graft survival.

Results: Two hundred and seventy-six patients received 320 PLTs. The most common cause of graft loss was hepatic artery thrombosis (n = 13, 29.6%). The most common cause of mortality was sepsis (n = 11, 29.7%). Univariate analysis showed that the following variables had a significant (p < .05) impact on patient survival: recipient age, weight, height, graft type (technical variant graft), transplant category (acute liver failure), the era of transplant, and invasive ventilation. The following variables had a significant (p < .05) impact on graft survival: recipient age, weight, height, transplant category (acute liver failure), and the era of transplant. Multivariate analysis precluded the era of transplant as the only significant factor for patient survival; patients transplanted after 2005 had significantly higher patient survival. No independent factor predicting graft survival was identified. For children transplanted after 2005, the only factor that predicted patient survival was pre-transplant invasive ventilation.

Conclusions: Our study suggests that the learning curve and pre-transplant invasive ventilation in the recipient have a significant impact on patient survival. The traditional view of worse outcomes of smaller PLT candidates should be changed.
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http://dx.doi.org/10.1111/petr.14139DOI Listing
September 2021

Synergistic action of silicon nanoparticles and indole acetic acid in alleviation of chromium (Cr) toxicity in Oryza sativa seedlings.

J Biotechnol 2021 Sep 14. Epub 2021 Sep 14.

Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Prayagraj-211004 (UP) India. Electronic address:

The present study investigates ameliorative effect of silicon nanoparticles (SiNPs) and indole acetic acid (IAA) alone and in combination against hexavalent chromium (Cr) toxicity in rice seedlings. The results of the study revealed protective effects of SiNPs and IAA against Cr toxicity. The 100μM of Cr imposed toxic effects in rice seedlings at morphological, physiological and biochemical levels which coincided with increased level of intracellular Cr and declined level of endogenous nitric oxide (NO). The Cr enhanced levels of superoxide radicals (SOR) (59.51% and 50.1% in shoot and root, respectively) and HO (19.5% and 23.69% in shoot and root, respectively). However, when SiNPs and IAA were applied to plants under Cr stress, they enhanced tolerance and defence mechanisms as manifested in terms of increased biomass, endogenous NO, photosynthetic pigments, and antioxidants level (ascorbate-glutathione cycle). It was also noticed that Cr arrested cell cycle at G2/M phase whereas growth was restored as compared to control when SiNPs and IAA were supplemented. Thus, the hypothesis that combined application of SiNPs and IAA will be effective in alleviating Cr toxicity is validated from the results of this study. Moreover, in SiNPs and IAA-mediated mitigation of Cr toxicity, endogenous NO has a positive role. The importance of the study will be that the combination of SiNPs and IAA can be utilized against heavy metal stress and even when supplied alone, they will enhance the crop productivity parameters with and without stress conditions.
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http://dx.doi.org/10.1016/j.jbiotec.2021.09.005DOI Listing
September 2021

Multiple sclerosis and its impact on dental care.

Br Dent J 2021 Sep 10;231(5):281-286. Epub 2021 Sep 10.

Department of Special Care Dentistry, King´s College NHS Foundation Trust, Dental Institute, Denmark Hill, London, SE5 9RS, UK.

Multiple sclerosis affects approximately 130,000 people in the UK. Due to the wide variation in the symptoms associated with this condition and their variable severity, the provision of dental care for affected patients must be determined by an individual's specific needs. This will often vary significantly over time. This paper reviews the aetiology, presentation and current management of multiple sclerosis, and explores the impact of these on oral health and the provision of dental care.
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http://dx.doi.org/10.1038/s41415-021-3333-0DOI Listing
September 2021

Germline BAP1 Mutation in a Family With Multi-Generational Meningioma With Rhabdoid Features: A Case Series and Literature Review.

Front Oncol 2021 24;11:721712. Epub 2021 Aug 24.

Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center-Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, Columbus, OH, United States.

Meningioma is the most common primary brain tumor, and recurrence risk increases with increasing WHO Grade from I to III. Rhabdoid meningiomas are a subset of WHO Grade III tumors with rhabdoid cells, a high proliferation index, and other malignant features that follow an aggressive clinical course. Some meningiomas with rhabdoid features either only focally or without other malignant features are classified as lower grade yet still recur early. Recently, inactivating mutations in the tumor suppressor gene have been associated with poorer prognosis in rhabdoid meningioma and meningioma with rhabdoid features, and germline mutations have been linked to a hereditary tumor predisposition syndrome (TPDS) predisposing patients primarily to melanoma and mesothelioma. We present the first report of a familial inactivating mutation identified after multiple generations of a family presented with meningiomas with rhabdoid features instead of with previously described loss-associated malignancies. A 24-year-old female presented with a Grade II meningioma with rhabdoid and papillary features treated with subtotal resection, adjuvant external beam radiation therapy, and salvage gamma knife radiosurgery six years later. Around that time, her mother presented with a meningioma with rhabdoid and papillary features managed with resection and adjuvant radiation therapy. Germline testing was positive for a pathogenic mutation in both patients. Sequencing of both tumors demonstrated biallelic inactivation the combination of germline mutation and either loss of heterozygosity or somatic mutation. No additional mutations implicated in oncogenesis were noted from either patient's germline or tumor sequencing, suggesting that the inactivation of was responsible for pathogenesis. These cases demonstrate the importance of routine tumor testing in meningioma with rhabdoid features regardless of grade, germline testing for patients with inactivated tumors, and tailored cancer screening in this population.
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http://dx.doi.org/10.3389/fonc.2021.721712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421801PMC
August 2021

Exogenous addition of silicon alleviates metsulfuron methyl induced stress in wheat seedlings.

Plant Physiol Biochem 2021 Aug 5;167:705-712. Epub 2021 Aug 5.

Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Prayagraj, 211004, UP, India. Electronic address:

Uncontrolled application of herbicides in the agricultural field poses a severe risk to crops by affecting their yields. Therefore, methods are required to reduce the toxic effects of herbicides in plants. Studies indicate that silicon (Si) provides tolerance and enhances defence mechanism of the plant against abiotic stress. But its role in alleviating Metsulfuron methyl (Meth) herbicide induced toxicity in wheat seedlings is still not known. This study highlighted the potential of exogenous addition of Si in the alleviation of toxic effect of Meth herbicide in wheat seedlings. The exposure of wheat seedlings to Meth herbicide reduced the growth, photosynthetic pigments, antioxidant enzyme activity and nitric oxide (NO) content. Further, Meth herbicide also increased cell death and decreased cell viability in root tips. However, addition of Si reversed Meth-induced these alterations. Moreover, Si also activates antioxidant system which helps in scavenging of free radicals generated under Meth herbicide stress in wheat seedlings. Application of Si to Meth treated wheat seedlings also up-regulated silicon transporter gene Lsi1 (silicon influx transporter) and some of the antioxidant enzyme genes. All together, the data indicate that Si has capability of alleviating Meth herbicide stress in wheat seedlings but it appears that endogenous NO has a positive role in this endeavour of Si.
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http://dx.doi.org/10.1016/j.plaphy.2021.07.031DOI Listing
August 2021
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