Publications by authors named "R P Rajesh"

261 Publications

Astragaloside IV- and nesfatin-1-encapsulated phosphatidylserine liposomes conjugated with wheat germ agglutinin and leptin to activate anti-apoptotic pathway and block phosphorylated tau protein expression for Parkinson's disease treatment.

Mater Sci Eng C Mater Biol Appl 2021 Oct 9;129:112361. Epub 2021 Aug 9.

Department of Chemical Engineering, National Chung Cheng University, Chia-Yi 62102, Taiwan, ROC.

Heap-up of α-synuclein (α-Syn) and its association with tau protein are esteemed to trigger the onset of Parkinson's disease (PD). The purpose of this study was to develop multi-functional liposomes incorporated with 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol, 1,2-dimyristoyl-sn-glycero-3-phosphocholine and phosphatidylserine (PS) to load astragaloside IV (AS-IV) and nestifin-1 (NF-1), followed by grafting with wheat germ agglutinin (WGA) and leptin (Lep) (WGA-Lep-AS-IV-NF-1-PS-liposomes) to protect dopaminergic neurons from apoptosis. Experimental results showed that increasing the mole percentage of DSPC and PS enhanced the particle size, particle stability and entrapment efficiency of AS-IV and NF-1, and reduced the drug releasing rate. Strong affinity of NF-1 to PS was evidenced by nuclear magnetic resonance spectroscopy. WGA-Lep-AS-IV-NF-1-PS-liposomes diminished transendothelial electrical resistance and improved the capacity of propidium iodide, AS-IV and NF-1 to penetrate the blood-brain barrier (BBB). Immunocytochemical staining exhibited the ability of functionalized liposomes to target Lep receptor and α-Syn in MPP-insulted SH-SY5Y cells. Western blots revealed a substantial reduction of α-Syn and phosphorylated tau protein in the anti-oxidative pathway through interaction with PS. During the course of treatment with WGA-Lep-AS-IV-NF-1-PS-liposomes, the combined activity of AS-IV and NF-1 and recognition capability simultaneously decreased the expression of Bax, and increased the expressions of Bcl-2, tyrosine hydroxylase and dopamine transporter. The liposomes carrying AS-IV and NF-1 can rescue degenerated neurons and are a promising formulation to achieve better PD management.
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http://dx.doi.org/10.1016/j.msec.2021.112361DOI Listing
October 2021

Antiviral properties of copper and its alloys to inactivate covid-19 virus: a review.

Biometals 2021 Aug 16. Epub 2021 Aug 16.

Department of Mechanical Engineering, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam, India.

Copper (Cu) and its alloys are prospective materials in fighting covid-19 virus and several microbial pandemics, due to its excellent antiviral as well as antimicrobial properties. Even though many studies have proved that copper and its alloys exhibit antiviral properties, this research arena requires further research attention. Several studies conducted on copper and its alloys have proven that copper-based alloys possess excellent potential in controlling the spread of infectious diseases. Moreover, recent studies indicate that these alloys can effectively inactivate the covid-19 virus. In view of this, the present article reviews the importance of copper and its alloys in reducing the spread and infection of covid-19, which is a global pandemic. The electronic databases such as ScienceDirect, Web of Science and PubMed were searched for identifying relevant studies in the present review article. The review starts with a brief description on the history of copper usage in medicine followed by the effect of copper content in human body and antiviral mechanisms of copper against covid-19. The subsequent sections describe the distinctive copper based material systems such as alloys, nanomaterials and coating technologies in combating the spread of covid-19. Overall, copper based materials can be propitiously used as part of preventive and therapeutic strategies in the fight against covid-19 virus.
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http://dx.doi.org/10.1007/s10534-021-00339-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366152PMC
August 2021

An empirical study of supply chain sustainability with financial performances of Indian firms.

Environ Dev Sustain 2021 Aug 9:1-25. Epub 2021 Aug 9.

Management Division, ABV-Indian Institute of Information Technology and Management (ABV-IIITM), Gwalior, India.

In this research, we examine empirically the impact of sustainable supply chain practices on financial performances, considering the case of Indian firms. Here, we use a sample of 25 Indian firms listed for their sustainability performances in the Thomson Reuters Environmental, Social and Governance (ESG) scores. The sustainability performance data have been accessed from the Bloomberg terminal, where the overall sustainability performance on ESG is measured as a discounted score on ESG considering various controversies on ESG reported for the firm. And for the study, we associate financial data using the profit indicators of firms. We perceive that the sustainable supply chain practices considering environmental, social and governance performances may not positively impact the financial performance measured by the Return on Asset (ROA) and Return on Equity (ROE), during the considered period of five years for the study. We construct the empirical model and use Partial Least Square (PLS) regression modeling to analyze the results. The study can be further extended for many Indian firms and for firms across different developing economies, as well. The major implications of this research are to observe for firms and their supply chains whether the implementation of Environmental, Social and Governance (ESG) practices can help them in achieving financial benefits, along with other competitive advantages. The research is built on the concept and theory of ecological modernization, which suggests for the economic benefits of environmentalism.
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http://dx.doi.org/10.1007/s10668-021-01717-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8349707PMC
August 2021

Glutathione- and apolipoprotein E-grafted liposomes to regulate mitogen-activated protein kinases and rescue neurons in Alzheimer's disease.

Mater Sci Eng C Mater Biol Appl 2021 Aug 10;127:112233. Epub 2021 Jun 10.

Department of Chemical Engineering, National Chung Cheng University, Chia-Yi 62102, Taiwan, ROC.

Neurodegenerative disorders, such as Alzheimer's disease (AD), present biomedical challenges due to inability of pharmaceuticals to permeate the blood-brain barrier (BBB) and lack of therapeutic specificity in definite sites against multiple pathologies. Phosphatidylcholine (PC)-liposomes carrying curcumin (CURC), quercetin (QU), epigallocatechin gallate (EGCG) and rosmarinic acid (RA) with crosslinked glutathione (GSH) and apolipoprotein E (ApoE) were fabricated to recognize brain microvascular endothelial cells and amyloid beta (Aβ), and reduce tau protein hyperphosphorylation for AD management. Addition of stearic acid to liposomal bilayers ameliorated particle stability, promoted drug entrapment efficiency, and prolonged drug release duration. The triple targeting liposomes boosted the capability of CURC, QU, EGCG and RA for crossing the BBB with the assistance of grafted GSH and ApoE and docking Aβ around SK-N-MC cells using ApoE and PC. Moreover, GSH-ApoE-PC-liposomes benefited the 4 medicines in simultaneously transporting to Aβ-insulted neurons, in functioning against hyperphosphorylated mitogen-activated protein kinases, including p-c-Jun N-terminal protein kinase, p-extracellular signal-regulated protein kinase 1/2 and p-p38, in downregulating tau protein at S202, caspase-3 and interleukin-6, and in upregulating p-cyclic adenosine monophosphate response element-binding protein. GSH-ApoE-PC-liposomes can be promising colloidal carriers in delivering CURC, QU, EGCG and RA to degenerated neural tissue in a controlled manner, targeting pathological factors for neuroprotection, and raising preclinical effectualness for AD treatment.
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http://dx.doi.org/10.1016/j.msec.2021.112233DOI Listing
August 2021

Glutathione Liposomes Carrying Ceftriaxone, FK506, and Nilotinib to Control Overexpressed Dopamine Markers and Apoptotic Factors in Neurons.

ACS Biomater Sci Eng 2021 07 30;7(7):3242-3255. Epub 2021 Jun 30.

Department of Chemical Engineering, National Chung Cheng University, Chia-Yi 62102, Taiwan, Republic of China.

Advances in liposomal formulation carrying multiple neuroprotective drugs, such as ceftriaxone (CEF), FK506, and nilotinib, can point toward an approach to obviating the difficulties in Parkinson's disease (PD) treatment. We prepared functionalized liposomes decorated with glutathione (GSH) to penetrate the blood-brain barrier (BBB) and cardiolipin (CL) to link up apoptotic neurons. Further, the effect of CEF-FK506-nilotinib-GSH-CL-liposomes on a PD model established by SH-SY5Y cells with 1-methyl-4-phenylpyridinium-induced neurotoxicity was investigated. An increment of the mole percentage of dihexadecyl phosphate and CL increased the particle size and the absolute value of ζ potential, improved the entrapment efficiency of CEF, FK506, and nilotinib, and reduced the drug-releasing rate. The toxicity studies revealed that CEF, FK506, and nilotinib-encapsulated liposomes could enhance the survival of SH-SY5Y cells. Western blot and immunofluorescence revealed that incorporation of CL in a lipid bilayer ameliorated the docking of CEF-FK506-nilotinib-GSH-CL-liposomes at α-synuclein (α-syn), indicating a better targeting capability of the liposomes to degenerated neurons. Treatment with CEF-FK506-nilotinib-GSH-CL-liposomes reduced the expression of Bax and α-syn and promoted the expression of Bcl-2, tyrosine hydroxylase, and the dopamine transporter. GSH- and CL-conjugated liposomes showed combined activity of targeting the BBB and α-syn and augmented the efficiency of the three drugs in rescuing dopaminergic neurons for neurodegenerative therapy.
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http://dx.doi.org/10.1021/acsbiomaterials.1c00555DOI Listing
July 2021
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