Publications by authors named "R P Parameswari"

20 Publications

Improvements in morphology and membrane stability obtained from -, a cryopreservation medium treated infertile smoker sperm cells - An study.

Toxicol Rep 2019 24;6:889-896. Epub 2019 Aug 24.

Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India.

Background: Plants are the major sources of antioxidants, which maintains oxidant: antioxidant state or is to protect from excessive reactive oxygen species in case of cryo medium selection though maintain sperm stability.

Bottleneck: Cryo preservation of infertile smokers is challengeable with the available medium due to over production of ROS hitches, were cause loss of sperm physiology. Hence, a novel medium is needed to store/protect sperm cells of infertile smokers where they attending/ongoing IVF or oncogenic surgical treatment.

Aim: The aim of this study, is to check the stability of sperm cells by TPP- bark () (E4) cryo medium preserved infertile smoker's against ROS/cryo injury- as a continuous study.

Materials And Methods: 42 infertile smoker's subjects with 28 control subjects were selected. Surface morphology (acrosome) of sperm by scanning electronic microscope, sperm membrane proteins by colorimetric method, sperm head and tail defects by CASA method and finally sperm cell stability is checking its zeta electric potential charges, were all done with E4 cryo medium treated frozen/thawed selected study subjects.

Results: Sperm morphology and zeta potential shows there is no damage along the stability of cells maintained during E4 medium cryopreservation in infertile subjects.

Summary: This is the first study is too established for infertile smokers sperm stability was checked for six months with E4 cryo medium.
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http://dx.doi.org/10.1016/j.toxrep.2019.08.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727244PMC
August 2019

Optimization of Human Semen Extender Components for Cryopreservation Using Statistical Tools.

Cryo Letters 2017 Nov/Dec;38(6):434-444

School of Biosciences and Technology, VIT University, Near Katpadi Road, Vellore, Tamil Nadu, India.

  BACKGROUND: Human sperm cell preservation is an important part of assisted reproductive technology (ART).

Objective: This study aimed to find the essential and significant components in semen preservation extender required to prolong the shelf life of human spermatozoa.

Materials And Methods: By using the statistical tool 'Plackett-Burman design' the significant components present in E4 extender (formulated in our previous study) was determined by reducing the unacceptable large number of trial experiments from the full factorial method.

Results: It was found that vitamin E, taurine and vitamin C were highly significant in maintaining the stability of sperm cells; and egg yolk, vitamin C and glucose were highly significant in sustaining the motility of the sperm cells. R values for the models were 0.9950 and 0.9960 respectively. In the optimized E4 extender 75 % and 81 % of the total motility was retained by the sperm cells from infertile and fertile samples respectively after cryopreservation. Also an increase in zeta potential was observed indicating a reduction in stability in both fertile and infertile sample (4 % and 18 % respectively) after cryopreservation in E4 medium, which was much less when compared with the sample preserved only with glycerol as cryoprotectant (11 % and 69 % for infertile and fertile samples respectively).

Conclusion: The major components present in E4 semen extender was successfully optimized for further use.
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June 2018

Human sperm DNA damage inhibition and antioxidant activity of T. arjuna bark: an in vitro study.

3 Biotech 2017 Jul 29;7(3):188. Epub 2017 Jun 29.

Gene Cloning and Technology, SBST, VIT University, Vellore-14, India.

Complimentary or natural antioxidant type of alternative medicine is developed worldwide to treat male infertility. The aim of this study is to the extraction of T. arjuna bark and activity against human sperm DNA damage in asthenoteratospermic smoker's subjects-an in vitro study. All preliminary and antioxidant assays (DPPH, HO, and total antioxidant, reducing power activity) were done. T. arjuna bark metal analysis was done with AAS. On the other hand, patients were asked to fill a direct questionnaire about smoking history; 25 infertile smokers were identified as asthenoteratospermic; 34 fertile non-smokers (control) were assessed for semen parameters by CASA, seminal plasma Zinc analysis by AAS, DNA fragmentation by colorimetric method and semen genomic DNA damage inhibition by modified non-enzymatic salting out extraction method. Most of the antioxidants are highly present in the aqueous extract; meanwhile, the major content in this extract is zinc 16 µg/g (Ca = 0.5 µg/g; Se = 2.2 µg/g and Mg = 1.6 µg/g) along with FT-IR peaks which also confirmed the metal presence. The semen parameters in smokers that were noticed are low sperm count and morphological changes. Meanwhile, in the seminal plasma of smokers, zinc and DNA fragmentation results were positively correlated with sperm morphology (p < 0.001). Repaired DNA bands were noticed in the in vitro study of aqueous T. arjuna bark, in smokers' semen. T. arjuna bark will act as cryo protector as well as great zinc supplementary to maintain sperm motility and morphology in smokers.
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http://dx.doi.org/10.1007/s13205-017-0853-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491448PMC
July 2017

Tea polyphenol-T. arjuna bark as sperm antioxidant extender in infertile smokers.

Cryo Letters 2017 Mar/Apr;38(2):95-99

Department of Industrial Biotechnology, School of Biosciences and Technology, VIT University, Vellore, India.

Background: Antioxidants protect spermatozoa against lipid peroxidation during freezing.

Objective: The study is designed to elucidate the suitable extender to preserve infertile semen of smokers against ROS damage using natural Tea polyphenol (T. arjuna bark extract).

Materials And Methods: Forty-two infertile subjects with smoking habit and 28 fertile subjects without smoking habit were considered for the study. Four semen extenders including our naturally derived antioxidant component were prepared and used to preserve semen sample from the study subjects for a period of one month. Standard semen parameters, biochemical and sperm DNA damage marker with inhibition were measured before and after cryopreservation.

Results: The motility and morphology of sperm cells were maintained better in E4 extender, and DNA damage is reduced.

Conclusion: Extender recipe with natural antioxidants (E3 and E4) was found to be apt for infertile semen preservation.
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July 2017

Novel mononuclear Cu (II) terpyridine complexes: Impact of fused ring thiophene and thiazole head groups towards DNA/BSA interaction, cleavage and antiproliferative activity on HepG2 and triple negative CAL-51 cell line.

Eur J Med Chem 2017 Jul 15;135:434-446. Epub 2017 Apr 15.

Chemical Laboratory, Central Leather Research Institute, Council of Scientific Industrial Research, Adyar, Chennai, India. Electronic address:

Two mononuclear copper (II) terpyridine complexes namely, [Cu(Btptpy) (ClO)](ClO) 1, and [Cu(Bttpy) (ClO)](ClO) 2, (Btptpy (L) = 4'-(Benzothiophene)-2,2':6',2″-terpyridine, Bttpy (L) = 4'-(Benzylthiazolyl)-2,2':6',2″-terpyridine) have been synthesized and characterized. Single crystal X-ray diffraction shows that, both ligands belong to monoclinic crystal system with space group P2/c (L) and P2/n (L). Absorption spectral titration, DNA melting study, circular dichroism and viscosity measurement reveal that, complex 1 and 2 bind with DNA through intercalation. In addition, interaction between the two copper (II) complexes and bovine serum albumin (BSA) has been studied by fluorescence titration, circular dichroism and their protease activity has been investigated using SDS-PAGE gel electrophoresis. Agarose (AGE) and SDS-PAGE gel electrophoresis reveals both complexes have good nucleolytic and proteolytic property in the presence of additive hydrogen peroxide. Both complexes shows remarkable cytotoxic property against triple negative CAL-51 human breast cancer cell line and hepatocellular carcinoma (HepG2) cancer cell lines and bears very less cytotoxicity towards liver normal cell line (Changs). DCF-DA and TBRAS assay also supported that complex 1 and 2 induces elevated level of reactive oxygen species (ROS) and oxidative stress in cancer cells than normal cell line. Furthermore, FACS analysis confirms complex 1 and 2 brings apoptosis by growth phase cell cycle arrest.
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http://dx.doi.org/10.1016/j.ejmech.2017.04.030DOI Listing
July 2017