Publications by authors named "Qunhui Wang"

102 Publications

Lactic acid production from co-fermentation of food waste and spent mushroom substance with Aspergillus niger cellulase.

Bioresour Technol 2021 Jun 2;337:125365. Epub 2021 Jun 2.

Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.

The feasibility of co-fermentation of food waste and spent mushroom substance for lactic acid with Aspergillus niger cellulase replacing commercial cellulase was explored. In this study, Enterococcus mundtii was used in this study because it could utilize hexose and pentose. When the ratio of food waste and spent mushroom substance was 1:2, lactic acid concentration was 39.22 g/L, 39.28% higher than the weighted average of experimental lactic acid concentrations, indicating that the co-fermentation had positive synergistic effects. Results showed 92.62% of sugars of pretreated spent mushroom substance was released by Aspergillus niger cellulase. Moreover, when Aspergillus niger cellulase was added into the lactic acid fermentation system at 24 h, lactic acid concentration reached 48.72 g/L, which was 22.97% higher than that of the control group with commercial cellulase, because of the disappearance of Veillonella and Saccharomycetales with the Aspergillus niger cellulase addition, thus making more substrates converted into lactic acid.
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http://dx.doi.org/10.1016/j.biortech.2021.125365DOI Listing
June 2021

Chloride removal from municipal solid waste incineration fly ash using lactic acid fermentation broth.

Waste Manag 2021 May 24;130:23-29. Epub 2021 May 24.

Chengdu Environmental Investment Group Co., LTD, Building 1, Tianfushijia, No. 1000 Jincheng Street, Chengdu, China.

As far as improvement of chlorine removal from fly ash by lactic acid fermentation broth (LAFB) was concerned, it is particularly important to explore the instinct mechanism and understand how leaching protocols (i.e. lactic acid addition amount and timing) affect the dechlorination efficiency. Results revealed that the WLL leaching protocol yielded the highest dechlorination efficiency (i.e. removed 98.7% of the total chlorine content of fly ash). The undissolved chlorine in fly ash residue might wrap inside the crystal structure of CaAlSiO(OH). Given that the chlorine removal from fly ash might prohibit by the newly formed calcium salt precipitation, exclusively increase the addition amount of LAFB (i.e. LLL protocol) did not necessarily stimulate the dechlorination efficiency. Conversely, it might accelerate the fly ash mass reduction (compared with WLL protocol), resulting in a high chlorine content in fly ash residue. Therefore, instead of increasing lactic acid strength, reducing the thickness of the calcium salt precipitation layer or breaking the crystal structure of CaAlSiO(OH) during the leaching process was suggested for efficient fly ash dechlorination.
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http://dx.doi.org/10.1016/j.wasman.2021.05.014DOI Listing
May 2021

Production of medium-chain fatty acid caproate from Chinese liquor distillers' grain using pit mud as the fermentation microbes.

J Hazard Mater 2021 May 5;417:126037. Epub 2021 May 5.

Co-Innovation Center of Efficient Processing and Utilization of Forestry Resources, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, Jiangsu 210037, China; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Henan Province Engineering Research Center for Biomass Value-added Products, School of Forestry, Henan Agricultural University, Zhengzhou 450002, China. Electronic address:

Chinese liquor distillers' grain (CLDG) is an abundant industrial organic waste showing high potential as feedstock for biofuel conversion. In this study, CLDG was used as substrate by microbial community in pit mud to produce medium-chain fatty acids (especially caproate). Simulated and real fermentation were used to evaluate the effect of ethanol and lactic acid being the electronic donors (EDs) during the anaerobic chain elongation (CE). The caproate concentration was achieved at 449 mg COD/g VS, with the corresponding high carbon selectivity at 37.1%. Microbial analysis revealed that the domestication of pit mud increased the abundance of Caproiciproducens (converting lactic acid into caproate) and Lactobacillus (producing lactic acid), leading to enhanced caproate production. The lactic acid conversion facilitated in full utilization of ethanol through CE consumption. The coexistence of EDs benefited the CE system and that this green energy production can be a promising high-performance biofuel donor for sustainable industrial production development.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126037DOI Listing
May 2021

Novel study on microbial fuel cells via a comprehensive bibliometric and dynamic approach.

Rev Environ Health 2021 May 11. Epub 2021 May 11.

Department of Environmental Engineering, University of Science and Technology Beijing, Beijing, China.

Microbial fuel cells (MFCs) are eco-friendly and useful bioelectrical devices that harness the natural metabolisms of microbes to produce electrical power directly from organic materials. In this study, a bibliometric analysis is conducted to evaluate MFC research from 2001 to 2018 on the basis of the Science Citation Index Expanded database. Overall, MFC research has experienced a dramatic increase over last 18 years, with an exponential growth in the accumulated number of publications. Most publications are closely related to the industrialization and commoditization of MFCs, along with environmental issues, which are currently the biggest global challenges in MFC studies. A small proportion (4.34%) of the scientific journals published more than half (54.34%) of the total articles in the MFC field. Articles from the top 10 countries/regions accounted for the majority (83.16%) of the total articles, clearly indicating that advanced MFC technologies are currently dominated by these countries/regions. Moreover, an increasing number of MFC researchers are considering two-chamber and three-chamber MFC reactions. In particular, they are focusing on environmental technology instead of merely improving the efficiency of electricity generation. Materials research in the MFC field is still a popular area worldwide, and many researchers have focused on novel and eco-friendly cathode and anode developments. Meanwhile, only a few MFC studies are concerned with biological research.
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http://dx.doi.org/10.1515/reveh-2020-0123DOI Listing
May 2021

Re-using ammonium-rich wastewater as a moisture conditioning agent during composting thermophilic period improves composting performance.

Bioresour Technol 2021 Jul 30;332:125084. Epub 2021 Mar 30.

Department of Environmental Science and Engineering, School of Space and Environment, Beihang University, Beijing 10191, China.

A weakly acidic ammonium-rich wastewater (STL) was intended to reuse as a moisture conditioning agent for composting to increase nitrogen content of compost. The influence of adding STL in the mesophilic period (MP), thermophilic period (TP), and cooling period (CP) on composting performance was investigated. Results revealed that organic degradation was strongly suppressed in MP, whereas no difference (p > 0.05) was observed between CP and control group (using tap water as moisture conditioning agent). The hydrolysis and mineralization of organic matter in TP were partly stimulated because reusing STL reduced free ammonia concentrations (<400 mg/L) of windrows. Additionally, the ammonium and nitrate nitrogen content of compost in TP increased by 71% and 425% without additional greenhouse gas emissions compared with control group. Therefore, ammonium-rich wastewater like STL could substitute tap water to condition compost moisture content and increase the nitrogen content of compost during the thermophilic composting period.
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http://dx.doi.org/10.1016/j.biortech.2021.125084DOI Listing
July 2021

Alteration of -Methyladenosine mRNA Methylation in a Rat Model of Cerebral Ischemia-Reperfusion Injury.

Front Neurosci 2021 16;15:605654. Epub 2021 Mar 16.

Department of Neurosurgery, The First Hospital of Jilin University, Changchun, China.

Aim: This study was conducted in order to reveal the alterations in the -methyladenosine (m6A) modification profile of cerebral ischemia-reperfusion injury model rats.

Materials And Methods: Rats were used to establish the middle cerebral artery occlusion and reperfusion (MCAO/R) model. MeRIP-seq and RNA-seq were performed to identify differences in m6A methylation and gene expression. The expression of m6A methylation regulators was analyzed in three datasets and detected by quantitative real-time polymerase chain reaction, western blot, and immunofluorescence.

Results: We identified 1,160 differentially expressed genes with hypermethylated or hypomethylated m6A modifications. The differentially expressed genes with hypermethylated m6A modifications were involved in the pathways associated with inflammation, while hypomethylated differentially expressed genes were related to neurons and nerve synapses. Among the m6A regulators, FTO was specifically localized in neurons and significantly downregulated after MCAO/R.

Conclusion: Our study provided an m6A transcriptome-wide map of the MACO/R rat samples, which might provide new insights into the mechanisms of cerebral ischemia-reperfusion injury.
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http://dx.doi.org/10.3389/fnins.2021.605654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009187PMC
March 2021

Brucine inhibits proliferation of glioblastoma cells by targeting the G-quadruplexes in the c-Myb promoter.

J Cancer 2021 2;12(7):1990-1999. Epub 2021 Feb 2.

Department of Neurosurgery, The First Hospital of Jilin University, Changchun, China.

The proto-oncogene plays an important role in cell proliferation, and its upregulation affects the development of glioblastomas. G-quadruplexes are secondary DNA or RNA structures that usually form in the promoter region of oncogenes, including , and regulate the expression of these genes. The traditional Chinese medicine, brucine, is a ligand of the G-quadruplexes located in the promoter region of . The present study investigated the therapeutic effects and mechanism of action of brucine in U87, LN18, and LN229 cells and . Our results showed that brucine suppressed the growth of these cells by arresting the cell cycle and reducing expression. Dual-luciferase reporter assays showed that brucine inhibited expression by targeting the guanine-rich sequence that forms G-quadruplexes in the promoter. Moreover, U87 tumors were suppressed by brucine in a tumor xenograft nude mouse model. Therefore, brucine is potentially effective for treating glioblastomas.
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http://dx.doi.org/10.7150/jca.53689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974541PMC
February 2021

Pterostilbene promotes mitochondrial apoptosis and inhibits proliferation in glioma cells.

Sci Rep 2021 Mar 18;11(1):6381. Epub 2021 Mar 18.

Department of Neurosurgery, First Hospital of Jilin University, Changchun, 130021, Jilin Province, China.

Glioma is the most general primary and lethal intracranial malignant tumor. Pterostilbene (PTE), an analog of stilbene and resveratrol, has attracted attention in recent years due to its significant antitumor activity in multiple solid tumors; however, its effect on drug-resistant glioma cells and the underlying mechanism have not yet been reported. In this study, we found that pterostilbene inhibited proliferation, induced intrinsic mitochondria-mediated apoptosis and caused S phase arrest, inhibited migration and excessive invasion in glioma cells. Pretreatment with the pan-caspase-inhibitor Z-VAD-FMK attenuated the PTE-induced apoptosis of glioma cells. Moreover, PTE significantly increased the production of reactive oxygen species (ROS) and reduce the mitochondrial membrane potential (MMP). Inhibition of ROS with N-acetyl-L-cysteine not only rescued PTE-induced reduction of cellular viability but also prevented glioma cell apoptosis. We also discovered ERK 1/2 and JNK signaling pathways were activated by PTE and contributed to induce glioma cell apoptosis. In addition, specific inhibitors of ERK 1/2 and JNK attenuated PTE-induced apoptosis. Besides, PTE significantly reduced tumor volume and prolonged median survival of tumor-bearing rats in vivo. In summary, the results of this study indicate that the anti-tumor effect of PTE on glioma cells may provide a new treatment option for glioma patients.
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http://dx.doi.org/10.1038/s41598-021-85908-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973728PMC
March 2021

Microbial lipid production from banana straw hydrolysate and ethanol stillage.

Environ Sci Pollut Res Int 2021 Feb 8. Epub 2021 Feb 8.

Department of Environmental Science and Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China.

In this study, the feasibility of banana straw (BS) hydrolysate as carbon source and reutilizing the pretreated liquor (PL) of BS in the Rhodosporidium toruloides fermentation was explored for the first time. When BS hydrolysate was used as the carbon source, total biomass concentration, lipid concentration, and lipid content under optimal conditions reached 15.52 g/L, 5.83 g/L, and 37.56% (w/w), respectively, which was similar to the results of pure sugar control. After detoxification, 50% PL can be returned to enzymatic hydrolysis and fermentation, and total biomass concentration, lipid concentration, and lipid content can reach 15.14 g/L, 5.59 g/L, and 36.91% (w/w). Then, ethanol stillage (ES) was used as the nitrogen source. The NaCl and glycerol of ES could promote lipid accumulation, reaching 7.52 g/L under optimized conditions. Finally, microbial lipid production from BS hydrolysate and ES without any additional nutrients was investigated, and the maximum total biomass concentration, lipid concentration, and lipid content were 13.55 g/L, 4.88 g/L, and 36.01% (w/w), respectively. Besides, the main compositions of microbial lipid produced were C16 and C18, and the biodiesel production from the microbial lipid could meet Chinese and US standard through theoretical numerical calculation.
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http://dx.doi.org/10.1007/s11356-021-12644-zDOI Listing
February 2021

Effect of co-digestion of tylosin fermentation dreg and food waste on anaerobic digestion performance.

Bioresour Technol 2021 Apr 11;325:124693. Epub 2021 Jan 11.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, PR China; Beijing Key Laboratory on Disposal and Resource Recovery of Industry Typical Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China; Tianjin College, University of Science and Technology Beijing, Tianjin 301830, PR China.

In this study, the effects of adding different food waste proportions (volatile solids ratio of 3:7, 5:5, 7:3) to tylosin fermentation dreg on anaerobic digestion were investigated. The results showed that the co-digestion group (294-399 mL·g-VS) increased methane production by 14.8%-55.5% compared with tylosin fermentation dreg alone-digestion (256 mL·g-VS). The correlation analysis showed that pH, total volatile fatty acids and acetic acid were the most important factors affecting cumulative methane production. Tylosin in the solid and liquid phases decreased significantly after anaerobic digestion, indicating that tylosin could be effectively removed by co-digestion, and the total removal rate was 68.2%-83.7%. Therefore, due to the satisfactory methane yield and the tolerable tylosin removal rate, it is feasible to make the co-digestion of tylosin fermentation dreg and food waste.
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http://dx.doi.org/10.1016/j.biortech.2021.124693DOI Listing
April 2021

Pretreatment to Posttreatment Hypoxia Inducible Factor-1α Ratios as a Potentially Predictive Marker for First-Line Treatment in Nonsmall Cell Lung Cancer Patients without Known Driver Mutations.

Genet Test Mol Biomarkers 2020 Dec;24(12):798-803

Oncology Department, Beijing Chest Hospital of Capital Medical University, Beijing, China.

Hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) are key angiogenic regulatory factors. The aim of this study was to identify the most useful prognostic angiogenic factors in advanced nonsmall cell lung cancer (NSCLC) without known driver gene mutations. Eligible patients were pathologically confirmed to have advanced NSCLC without known driver mutations. All patients were treated with standard first-line chemotherapy ± bevacizumab. Serum concentrations of HIF-1α, VEGF, sVEGFR1, sVEGFR2, and endostatin were measured via enzyme-linked immunosorbent assays (ELISAs) prior to and after two cycles of treatment. Area under the curve (AUC) and optimal cutoff values were calculated by receiver operator characteristic curve (ROC) analyses. The parameters that predicted survival were evaluated by univariate and multivariate Cox proportional hazard analyses. A total of 47 patients were included in this study. HIF-1α levels decreased significantly after treatment in the nonprogressing (partial response/stable disease) patient group (707.94 vs. 355.53 pg/mL,  = 0.002), but increased levels were seen in patients with progressive disease, however, the extent of change did not reach significance (173.70 vs. 416.34 pg/mL,  = 0.078). An HIF-1α ratio of 1.18 was chosen as the best point to predict treatment response through ROC analyses. Via univariate and multivariate analyses, we found that patients with a HIF-1α ratio ≥1.18 after treatment were significantly more likely to have a prolonged progression-free survival (PFS, HR 0.303, 95% CI: 0.153-0.603,  = 0.001) and overall survival (OS, HR 0.436, 95% CI: 0.153-0.603,  = 0.025). We identified the pretreatment to posttreatment HIF-1α ratio as a promising predictor for PFS and OS in NSCLC patients without known driver mutations.
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http://dx.doi.org/10.1089/gtmb.2020.0173DOI Listing
December 2020

Effect of ultrasonic pretreatment on chain elongation of saccharified residue from food waste by anaerobic fermentation.

Environ Pollut 2021 Jan 28;268(Pt B):115936. Epub 2020 Oct 28.

Co-Innovation Center of Efficient Processing and Utilization of Forestry Resources, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China. Electronic address:

Converting biowaste into value-added products has raised the researchers' interests. In this study, bioconversion was applied to produce chain acids from food waste by anaerobic fermentation. To improve the caproic acid production, different pretreatments (i.e., ultrasonic, hydrothermal, and alkaline-thermal) were used for investigating their effects on the acidogenic production and microbial communities. The results showed that ultrasonic and hydrothermal pretreatments (207.8 and 210.1 mg COD/g VS, respectively) were very efficient for enhancing the caproic acid production, compared to the alkaline-thermal pretreated samples and control samples (72.6 and 97.5 mg COD/g VS, respectively). The ultrasonic pretreatment was beneficial for reducing volatile fatty acids (VFAs) during the caproic acid production, resulting in converting more lactic acid to caproic acid by adding the hydrothermal pretreatment. The microbial community analysis showed that the acidogenic bacteria Caproiciproducens dominated the fermentation in this bioconversion process of food waste into chain acids. The Caproiciproducens mainly degraded the proteins and carbohydrates from the saccharified residues of food waste to produce caproic acids through chain elongation procedure. The investigation and optimized method may help develop the bioconversion technology for producing VFAs products from food wastes.
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http://dx.doi.org/10.1016/j.envpol.2020.115936DOI Listing
January 2021

Comprehensive epigenetic analysis of m6A modification in the hippocampal injury of diabetic rats.

Epigenomics 2020 10 28;12(20):1811-1824. Epub 2020 Oct 28.

Department of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.

To study RNA N6-methyladenosine (m6A) modification in the diabetic hippocampus. Behavioral tests and staining were performed to evaluate the damage to the diabetic hippocampus in model rats. Western blotting was performed to investigate the expression of methylation-related enzymes, and flow cytometry was used to demonstrate HT22 cell apoptosis. M6A and RNA sequencing analyses were conducted to profile m6A-tagged transcripts in the diabetic hippocampus. The rat models of diabetes mellitus suffered from cognitive disorders and hippocampal neuron damage. High glucose levels altered the expression of methylation-related enzymes. A total of 4890 differentially methylated m6A peaks and 63 differentially expressed genes and differentially methylated m6A sites were identified. The findings suggest that m6A modification is altered in the diabetic hippocampus and provide new insight into diabetic hippocampal injury.
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http://dx.doi.org/10.2217/epi-2020-0125DOI Listing
October 2020

Effect of yeast addition on the biogas production performance of a food waste anaerobic digestion system.

R Soc Open Sci 2020 Aug 5;7(8):200443. Epub 2020 Aug 5.

Department of Environmental Science and Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, People's Republic of China.

Food waste contains numerous easily degradable components, and anaerobic digestion is prone to acidification and instability. This work aimed to investigate the effect of adding yeast on biogas production performance, when substrate is added after biogas production is reduced. The results showed that the daily biogas production increased 520 and 550 ml by adding 2.0% (volatile solids; VS) of activated yeast on the 12th and 37th day of anaerobic digestion, respectively, and the gas production was relatively stable. In the control group without yeast, the increase of gas production was significantly reduced. After the second addition of substrate and yeast, biogas production only increased 60 ml compared with that before the addition. After fermentation, the biogas production of yeast group also increased by 33.2% compared with the control group. Results of the analysis of indicators, such as volatile organic acids, alkalinity and propionic acid, showed that the stability of the anaerobic digestion system of the yeast group was higher. Thus, the yeast group is highly likely to recover normal gas production when the biogas production is reduced, and substrate is added. The results provide a reference for experiments on the industrialization of continuous anaerobic digestion to take tolerable measures when the organic load of the feed fluctuates dramatically.
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http://dx.doi.org/10.1098/rsos.200443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481686PMC
August 2020

Comparative study on inorganic Cl removal of municipal solid waste fly ash using different types and concentrations of organic acids.

Chemosphere 2020 Dec 23;261:127754. Epub 2020 Jul 23.

Department of Urban and Environmental Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

In this study, different organic acids-such as citric, acetic, lactic, propionic, and butyric acid-were evaluated to ascertain the optimum leaching solvent for dechlorinating fly ash. Results suggest that the acid type, concentration, and interactions between both parameters contributed significantly to the variations in the efficiency of fly ash dechlorination. Simple main-effect analysis suggested that a higher acid concentration yields better dechlorination efficiency. However, improvements in dechlorination efficiency did not necessarily yield a low chlorine content leaching residue because in a specific acid concentration region, the increased acid concentration may also accelerate the mass reduction rate of the leaching residue. Experimental results also demonstrate that citric and acetic acid yield the highest dechlorination efficiency, followed by propionic and butyric acid. The least dechlorination efficiency of lactic acid could be attributed to the formation of precipitate (i.e. calcium lactate) which might cover the chlorides and reduce the contact area of intimal chlorides with the leaching solvent. Therefore, a specific concentration of organic matter fermentation broth rich in citric and acetic radicals may present itself as an ideal water substitute for fly ash dechlorination.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127754DOI Listing
December 2020

Effect of liquid digestate recirculation on the ethanol-type two-phase semi-continuous anaerobic digestion system of food waste.

Bioresour Technol 2020 Oct 25;313:123534. Epub 2020 May 25.

Department of Environmental Engineering, School of Energy and Environmental Engineering, Beijing Key Laboratory on Disposal and Resource Recovery of Industry Typical Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China; Tianjin College, University of Science and Technology Beijing, Tianjin 301830, PR China.

This study set up four groups for semi-continuous 150-days experiment to explore the effect of liquid digestate recirculation on the food waste ethanol-type anaerobic digestion system. Results showed that this operation improved the maximum organic load rates to 6.0 g-VS·L·d, and increased the average alkalinity of methanogenic phase under high load condition by 1.3 times. Total volatile fatty acids/total alkalinity (threshold value was approximately 0.5) could be used as an early warning indicator of methanogenic phase instability. Besides, approximately 64.5% of bacterial species in the hydrolysed acidified phase of ethanol-type liquid digestate recirculation group originated from the recirculated liquid digestate, which enriched the diversity of microbial community, thereby improving the hydrolysis acidification efficiency. Therefore, liquid digestate recirculation improved the stability of system in terms of alkalinity and microecology and then increased the maximum organic load rates.
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http://dx.doi.org/10.1016/j.biortech.2020.123534DOI Listing
October 2020

Weighted gene co-expression network analysis identified six hub genes associated with rupture of intracranial aneurysms.

PLoS One 2020 21;15(2):e0229308. Epub 2020 Feb 21.

Department of Neurosurgery, The First Hospital of Jilin University, Changchun, Jilin, P. R. China.

Intracranial aneurysms (IAs) are characterized by localized dilation or ballooning of a cerebral artery. When IAs rupture, blood leaks into the space around the brain to create a subarachnoid hemorrhage. The latter is associated with a higher risk of disability and mortality. The aims of this study were to gain greater insight into the pathogenesis of ruptured IAs, and to clarify whether identified hub genes represent potential biological markers for assessing the likelihood of IA progression and rupture. Briefly, the GSE36791 and GSE73378 datasets from the National Center of Biotechnology Information Gene Expression Omnibus database were reanalyzed and subjected to a weighted gene co-expression network analysis to test the association between gene sets and clinical features. The clinical significance of these genes as potential biomarkers was also examined, with their expression validated by quantitative real-time PCR. A total of 14 co-expression modules and 238 hub genes were identified. In particular, three modules (labeled turquoise, blue, and brown) were found to highly correlate with IA rupture events. Additionally, six potential biomarkers were identified (BASP1, CEBPB, ECHDC2, GZMK, KLHL3, and SLC2A3), which are strongly associated with the progression and rupture of IAs. Taken together, these findings provide novel insights into potential molecular mechanisms responsible for IAs and they highlight the potential for these particular genes to serve as biomarkers for monitoring IA rupture.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229308PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034829PMC
May 2020

Toll-like receptor-4 pathway as a possible molecular mechanism for brain injuries after subarachnoid hemorrhage.

Int J Neurosci 2020 Sep 14;130(9):953-964. Epub 2020 Jan 14.

Department of Neurosurgery, The First Hospital of Jilin University, Changchun, Jilin, P. R. China.

Subarachnoid hemorrhage (SAH) is known as an acute catastrophic neurological disease that continues to be a serious and significant health problem worldwide. The mechanisms contributing to brain injury after SAH remain unclear despite decades of study focusing on early brain injury (EBI) and delayed brain injury (DBI). Neuroinflammation is a well-recognized consequence of SAH and may be responsible for EBI, cerebral vasospasm, and DBI. Toll-like receptors (TLRs) play a crucial role in the inflammatory response by recognizing damage-associated molecular patterns derived from the SAH. TLR4 is the most studied Toll-like receptor and is widely expressed in the central nervous system (CNS). It can be activated by the extravasated blood components in myeloid differentiation primary response-88/Toll/interleukin-1 receptor-domain-containing adapter-inducing interferon-β (MyD88/TRIF)-dependent pathway after SAH. Transcription factors, such as nuclear factor-κB (NF-κB), mitogen-activated protein kinase (MAPK) and interferon regulatory factor (IRF), that regulate the expression of proinflammatory cytokine genes are initiated by the activation of TLR4, which cause the brain damage after SAH. TLR4 may therefore be a useful therapeutic target for overcoming EBI and DBI in post-SAH neuroinflammation, thereby improving SAH outcome. In the present review, we summarized recent findings from basic and clinical studies of SAH, with a primary focus on the biological characteristics and functions of TLR4 and discussed the mechanisms associated with TLR4 signaling pathway in EBI and DBI following SAH.
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http://dx.doi.org/10.1080/00207454.2019.1709845DOI Listing
September 2020

Effect of ethanol pre-fermentation on organic load rate and stability of semi-continuous anaerobic digestion of food waste.

Bioresour Technol 2020 Mar 10;299:122587. Epub 2019 Dec 10.

Department of Environmental Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, PR China; Beijing Key Laboratory on Disposal and Resource Recovery of Industry Typical Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China; Tianjin College, University of Science and Technology Beijing, Tianjin 301830, PR China.

This study investigated the effects of ethanol pre-fermentation on the organic load rate (OLR) and stability of single-phase semi-continuous anaerobic digestion system of food waste. Results revealed that the pre-fermentation group tolerated higher OLR, with a maximum OLR of 5.5 g-VS·L·d. Moreover, the abundance of microbial community in relation to amino acid degradation in the pre-fermentation group was significantly higher than that in the control group, which made a higher concentration of ammonia nitrogen and caused a stronger system stability and higher alkalinity. Redundancy analysis was performed to explore the factors affecting system instability under high OLR. The instability of control group was found to be significantly influenced by the total volatile fatty acids (TVFA), whereas the pre-fermentation group was affected by total ammonia nitrogen and TVFA. Therefore, if the OLR of the pre-fermentation group is to be further increased, ammonia nitrogen inhibition should also be considered.
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http://dx.doi.org/10.1016/j.biortech.2019.122587DOI Listing
March 2020

Methane production from food waste via mesophilic anaerobic digestion with ethanol pre-fermentation: Methanogenic pathway and microbial community analyses.

Bioresour Technol 2020 Feb 21;297:122450. Epub 2019 Nov 21.

Department of Environmental Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, PR China; Beijing Key Laboratory on Disposal and Resource Recovery of Industry Typical Pollutants, University of Science and Technology Beijing, Beijing 100083, PR China. Electronic address:

To investigate the methanogenic pathway and microbial community in a mesophilic anaerobic digestion (AD) system with food waste (FW) ethanol pre-fermentation (EP), two semi-continuous AD systems were operated by feeding FW with (PSR) and without EP (control). In this study, δC-ethanol was supplemented as solo substrate for AD sludge when the reactors operation stabilized to analyze the methanogenic pathways. The results suggested that approximately 59.3% of methane was produced from acetotrophic methanogens, while 40.7% was formed by hydrogenotrophic methanogens in the PSR group. On the other hand, compared with control, methane produced via CO reduction pathway was increased by 4.70%. Meanwhile, the composition variations of the microbial community in AD supported the above conclusion, since the relative abundances of Clostridium and Methanobacterium were enhanced by 7.6% and 10.2%, respectively in PSR reactor. These results provided a theoretical basis for AD applications and biogas yield improvements with EP process.
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http://dx.doi.org/10.1016/j.biortech.2019.122450DOI Listing
February 2020

[A Single Center Analysis of Advanced Non-small Cell Lung Cancer Patients Treated with Immunotherapy in Real-world Practice].

Zhongguo Fei Ai Za Zhi 2019 Nov;22(11):687-695

Department of General Medicine, Beijing Chest Hospital, Capital Medical University/Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China.

Background: In recent years, a number of clinical trials have shown that immunocheckpoint inhibitors (ICI) have brought survival benefits to patients with advanced non-small cell lung cancer (NSCLC), however, such clinical trials comprise cohorts selected based on strict and complex entry and exclusion criteria, and the results cannot fully reflect the real world situation. The purpose of this study was to investigate the clinical efficacy and safety of immunotherapy in the real world, as well as possible prognostic factors.

Methods: Patients with advanced NSCLC receiving immunotherapy in Beijing Chest Hospital from January 2017 to July 2019 were retrospectively collected, and the following information were collected: curative effect, progression-free surival (PFS) and adverse reactions. The occurrence of adverse reactions and clinical curative effect and prognosis factors that may be relevant were explored.

Results: 34 patients were enrolled in this study, median PFS was 5.66 months (95%CI: 4.48-6.84), grade 1-2 and 3-4 incidence of adverse events was 61.71% (22/34) and 14.71% (5/34), there were 3 patients (8.82%) experienced fatal immune related adverse events (irAE), 2 cases were immune associated pneumonia, 1 case was immune related myocarditis. Univariate analysis showed that tumor-node-metastasis (TNM) stage and metastatic site were correlated with median PFS (P<0.05), and multivariate analysis showed that patients with extrapulmonary metastasis (OR=6.42, P=0.029) and pleural metastasis (OR=14.14, P=0.006) had shorter median PFS.

Conclusions: In the real world, immunotherapy has good efficacy in patients with advanced NSCLC, but the incidence of severe irAE is also higher. Distant metastasis and pleural metastasis are poor prognostic factors for advanced NSCLC patients receiving immunotherapy.
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http://dx.doi.org/10.3779/j.issn.1009-3419.2019.11.02DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885419PMC
November 2019

The bibliometric analysis and review of dioxin in waste incineration and steel sintering.

Environ Sci Pollut Res Int 2019 Dec 19;26(35):35687-35703. Epub 2019 Nov 19.

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

Facing the common treatment problems of dioxin whose major sources come from waste incineration and steel sintering, we handled a massive literature dataset from the Web of Science database and analyzed the research hotspot and development trend in this field in the past 40 years by bibliometric method. The result indicates that the field of dioxins generated from waste incineration and steel sintering has entered a stage of rapid development since 1990. China occupies a leading position in terms of comprehensive strength with the largest publications output as well as a greater influence in recent years. The most productive institutions and journals are Zhejiang University and Chemosphere, respectively. In addition, the most commonly used keywords in statistical analysis are "fly ash," "emission control," "risk assessment," "congener profile," "formation mechanisms," "sources," "catalysis," and "inhibition," which reflects the current main research direction in this field. The similarities and differences of dioxins generated in waste incineration and steel sintering are reviewed in this paper, which will provide guidance for the future research.
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http://dx.doi.org/10.1007/s11356-019-06744-0DOI Listing
December 2019

Synergistic effect from anaerobic co-digestion of food waste and Sophora flavescens residues at different co-substrate ratios.

Environ Sci Pollut Res Int 2019 Dec 19;26(36):37114-37124. Epub 2019 Nov 19.

Department of Environmental Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China.

When food waste (FW) undergoes anaerobic digestion, the hydrolysis rate is rapid, and thus causes system instability. Sophora flavescens residues (SFRs) are rich in complex hydrolysed substances, such as lignocellulosic material. When combined FW and SFRs can effectively improve the stability of digestion systems and increase biogas yields. In this work, batch anaerobic experiments were conducted at different co-substrate ratios to investigate the performance of co-digestion and the synergistic effect of FW and SFRs. The co-digestion of the two substrates exerted synergistic effects on biogas production and the highest synergy was 120.8%. After digestion, the ratio of hydrolysed chemical oxygen demand (COD) to the entire COD (RCOD) of the co-digestion group was 1.08 times that of the single FW group, which indicated the co-digestion promoted the hydrolysis of substrates. Moreover, the hydrolysis rate constant (k) of co-digestion group increased by 4.10 times in comparison with that of the single FW group, which indicated the co-digestion increased the hydrolysis rate. In other words, the synergistic effect mainly occurred in the hydrolysis acidification process.
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http://dx.doi.org/10.1007/s11356-019-06399-xDOI Listing
December 2019

Ceramsite production from sediment in Beian River: characterization and parameter optimization.

R Soc Open Sci 2019 Aug 14;6(8):190197. Epub 2019 Aug 14.

Department of Environmental Engineering, University of Science and Technology, Beijing 100083, People's Republic of China.

In order to realize pollution control and resource recovery, sediment from Beian River in Mudanjiang City China was used for ceramsite production. The maximum content of total nitrogen (TN), total phosphorus (TP) and organic matter (OM) in sediments of Beian River were 2975 mg kg, 2947 mg kg and 29.6%, respectively. So, it should be treated properly for resource utilization. The orthogonal experiment of (4) was adopted to determine the best conditions for ceramsite production and the result demonstrated that the sewage sludge ratio of 15%, binder ratio of 5%, pre-heating temperature of 450°C, sintering temperature of 1150°C and firing time of 23 min were the optimum conditions. The corresponding product met with the standard of CJ/T 299-2008 and the heavy metal leaching experiment showed it was lower than the threshold of China's industrial standard. Thus, it demonstrated that ceramsite production was a feasible way for utilization of sediment.
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http://dx.doi.org/10.1098/rsos.190197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731694PMC
August 2019

Volatile fatty acids production from saccharification residue from food waste ethanol fermentation: Effect of pH and microbial community.

Bioresour Technol 2019 Nov 7;292:121957. Epub 2019 Aug 7.

Department of Environmental Engineering, University of Science and Technology Beijing, 100083, China; Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing 100083, China. Electronic address:

In this study, residue from saccharification and centrifugation of food waste ethanol fermentation was used as substrate to produce volatile fatty acids. The effects of different pH (5.5, 6.5, and uncontrolled) on the VFAs concentration, composition, acidogenic efficiency and microbial community distribution were investigated. The results showed that the highest concentration of VFAs was 267.8 ± 8.9 mg COD/g VS at pH of 6.5, and the highest percentage of butyric acid (79.8%) was followed by propionic acid and acetic acid at the end of the reaction. Microbial analysis showed that the contents of Vagococcus and Actinomyces increased, while the contents of Bacteroides and Fermentimonas decreased during anaerobic fermentation. The comparative high pH induced the accumulation of butyric acid. This study provides a new idea for the step anaerobic fermentation of food waste to produce alcohol and acid simultaneously.
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http://dx.doi.org/10.1016/j.biortech.2019.121957DOI Listing
November 2019

A review on characteristics of food waste and their use in butanol production.

Rev Environ Health 2019 Dec;34(4):447-457

Beijing Key Laboratory on Disposal and Resource Recovery of Industry Typical Pollutants, University of Science and Technology Beijing, Beijing, China.

Biobutanol offers several advantages and a larger market, that make it a biofuel to be studied with great interest. In fact, butanol has an energy content similar to that of gasoline, and it can be used as an alternative fuel to gasoline. It is a biofuel that is safe for the environment. The optimization of the production of butanol thus appears as an attractive option. Butanol production from food waste (FW) is a process for carbon recovery and a method for solid waste recycling. Recently, the use of FW and food processing waste (FPW) as raw material for the production of butanol has attracted much interest. However, an efficient fermentation process is vital to improve the production of biobutanol. To the best of our knowledge, no review on butanol production from FW has been presented so far. Thus, this review focuses on the characteristics of FW and its potential to produce butanol. In addition, the main factors that affect their use for the production of butanol are also discussed.
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http://dx.doi.org/10.1515/reveh-2019-0037DOI Listing
December 2019

Metabolic analysis of efficient methane production from food waste with ethanol pre-fermentation using carbon isotope labeling.

Bioresour Technol 2019 Nov 20;291:121849. Epub 2019 Jul 20.

Tianjin College, University of Science and Technology Beijing, Tianjin 301830, PR China.

Anaerobic digestion (AD) has been widely applied as an economic option for food waste (FW) treatment. In this study, the group treated with ethanol pre-fermentation (EP) for 12 h (EP12) exhibited the highest cumulative biogas yield (206 mL/g-volatile solid) during AD process and therefore it was used to illuminate the underlying metabolic processes of AD with EP. Carbon isotope labeled glucose was supplemented to FW substrate, and the EP process was found to alleviate the acidification inhibition with conducting extremely high carbon flux towards ethanol formation (43.7%). Then an efficient acetogenesis phase was also observed in EP12 group, because of high carbon conversion rate from ethanol to acetate. Overall, higher carbon conversion rate to methane (90.1%) during methanogenesis was found in the AD system with EP than in the control experiment (80.3%). Thus, we quantitatively confirmed that EP affects the AD metabolism of FW in terms of carbon flow distribution.
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http://dx.doi.org/10.1016/j.biortech.2019.121849DOI Listing
November 2019

An excellent alternative composite modifier for cathode catalysts prepared from bacterial cellulose doped with Cu and P and its utilization in microbial fuel cell.

Bioresour Technol 2019 Oct 18;289:121661. Epub 2019 Jun 18.

Department of Environmental Engineering, University of Science and Technology Beijing, 100083, China; Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing 100083, China.

In this study, bacterial cellulose doped with phosphorus and copper via freeze-drying and high-temperature pyrolysis was used to prepare MFC cathode catalysts. After a series of characterization, the synthesized catalyst showed a three-dimensional network with a specific surface area of 580.09 m/g. Due to the doping of Cu and P, more active sites were induced in the pores of bacterial cellulose and subsequently improved catalytic activity. The prepared catalyst was coated on the air cathode surface of the MFC to obtain the maximum output power and current density of 1177.31 mW/m and 6.73 A/m, respectively, which were higher than those of Pt (1044.93 mW/m and 6.02 A/m). This work aimed to improve bioelectrical generation in MFC and find alternative commercial Pt catalysts.
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http://dx.doi.org/10.1016/j.biortech.2019.121661DOI Listing
October 2019

Microbial lipid production from food waste saccharified liquid under two-stage process.

Bioresour Technol 2019 Oct 12;289:121626. Epub 2019 Jun 12.

Department of Environmental Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China; Beijing Key Laboratory on Resource-oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China. Electronic address:

This study aimed to clarify the composition changes of food waste after being placed for few days and propose a two-stage fermentation method to effectively convert food waste saccharified liquid (FWSL) into lipids by Rhodosporidium toruloides. Food waste generally needs 3-5 days to be transported and stored before treatment. The lactic acid concentration of FWSL produced from 5-days-placed-at-room-temperature food waste reached to 15 g/L. Lactic acid promoted yeast proliferation, and its main mechanism was the conversion of lactic acid into pyruvic acid, which could provide energy for yeast growth through TCA cycle. The optimal lipid concentration in the two-stage fermentation reached to 9.19 g/L, and lipid yield amounted to 0.204 g lipid/g total sugar; the values increased by 44.27% and 60.63%, respectively, when compared with those in traditional fermentation. This study could provide a strategy for food waste treatment closer to industrial production.
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http://dx.doi.org/10.1016/j.biortech.2019.121626DOI Listing
October 2019

Production of butanol from biomass: recent advances and future prospects.

Environ Sci Pollut Res Int 2019 Jul 21;26(20):20164-20182. Epub 2019 May 21.

Department of Environmental Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, China.

At present, diminishing oil resources and increasing environmental concerns have led to a shift toward the production of alternative biofuels. In the last few decades, butanol, as liquid biofuel, has received considerable research attention due to its advantages over ethanol. Several studies have focused on the production of butanol through the fermentation from raw renewable biomass, such as lignocellulosic materials. However, the low concentration and productivity of butanol production and the price of raw materials are limitations for butanol fermentation. Moreover, these limitations are the main causes of industrial decline in butanol production. This study reviews butanol fermentation, including the metabolism and characteristics of acetone-butanol-ethanol (ABE) producing clostridia. Furthermore, types of butanol production from biomass feedstock are detailed in this study. Specifically, this study introduces the recent progress on the efficient butanol production of "designed" and modified biomass. Additionally, the recent advances in the butanol fermentation process, such as multistage continuous fermentation, metabolic flow change of the electron carrier supplement, continuous fermentation with immobilization and recycling of cell, and the recent technical separation of the products from the fermentation broth, are described in this study.
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http://dx.doi.org/10.1007/s11356-019-05437-yDOI Listing
July 2019