Publications by authors named "Qunfang Yang"

17 Publications

  • Page 1 of 1

Multifaceted roles of a bioengineered nanoreactor in repressing radiation-induced lung injury.

Biomaterials 2021 Aug 27;277:121103. Epub 2021 Aug 27.

Department of Pharmacology, College of Pharmacy and Laboratory Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, PR China. Electronic address:

Radiation-induced lung injury (RILI) is a potentially fatal and dose-limiting complication of thoracic cancer radiotherapy. However, effective therapeutic agents for this condition are limited. Here, we describe a novel strategy to exert additive effects of a non-erythropoietic EPO derivative (ARA290), along with a free radical scavenger, superoxide dismutase (SOD), using a bioengineered nanoreactor ([email protected]). ARA290-chimeric nanoreactor makes SOD present in a confined reaction space by encapsulation into its interior to heighten stability against denaturing stimuli. In a RILI mouse model, intratracheal administration of [email protected] was shown to significantly ameliorate acute radiation pneumonitis and pulmonary fibrosis. Our investigations revealed that [email protected] performs its radioprotective effects by protecting against radiation induced alveolar epithelial cell apoptosis and ferroptosis, suppressing oxidative stress, inhibiting inflammation and by modulating the infiltrated macrophage phenotype, or through a combination of these mechanisms. In conclusion, [email protected] is a potential therapeutic agent for RILI, and given its multifaceted roles, it may be further developed as a translational nanomedicine for other related disorders.
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http://dx.doi.org/10.1016/j.biomaterials.2021.121103DOI Listing
August 2021

In Situ biomimetic Nanoformulation for metastatic cancer immunotherapy.

Acta Biomater 2021 Jul 27. Epub 2021 Jul 27.

Department of Pharmacology, College of Pharmacy and Laboratory Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, P.R. China. Electronic address:

Metastasis is the leading cause of death in cancer patients. Eliciting anti-tumor immune responses against lung metastasis is hindered by the immunosuppressive microenvironment. This study explored a biomimetic nanoformulation, comprising a nanovaccine (OP) that delivers tumor antigens and adjuvants spatially and temporally in a virus-like manner, and a pulmonary surfactant-biomimetic liposome with an immunomodulator, JQ1 (PS-JQ1). The findings of this study showed that intratracheal administration of OP+PS-JQ1 activated lung immune cells without concomitant excess inflammation, enhanced tumor antigen cross-presentation, generated a significantly high antigen-specific CD8 T cell response, and reshaped the immunocellular composition in B16 melanoma tumor-bearing lung. OP+PS-JQ1 nanoformulation exhibited a striking immunotherapeutic efficacy, induced local and systemic tumor suppression, improved survival of mice, initiated immune memory that prevents recurrence of secondary tumors. This stable and nontoxic nanoformulation provides a simple, flexible, and robust strategy for augmenting anti-tumor immunity for metastatic cancer. STATEMENT OF SIGNIFICANCE: Egg glue proteins are produced by female insects, which can make the eggs firmly attached to the oviposition sites, not affected by wind and rain. However, genes encoding insect egg glue proteins have not yet been reported, and the molecular mechanism underpinning their adhesion is still unknown. Our study makes a significant contribution to the literature as it identifies the sequence, structure, adhesive property, and mechanism of silkworm egg glue protein. Furthermore, it outlines key insights into the structure-function relationships associated with egg glue proteins. We believe that this paper will be of interest to the readership of your journal as it identifies the first complete sequence of insect egg glue proteins, thereby highlighting their potentials future applications in both the biomedical and technical fields.
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http://dx.doi.org/10.1016/j.actbio.2021.07.055DOI Listing
July 2021

Fitness Costs of Chlorantraniliprole Resistance Related to the Overexpression in the (Lepidoptera: Noctuidae).

Int J Mol Sci 2021 May 10;22(9). Epub 2021 May 10.

Biorational Pesticide Research Laboratory, Agricultural College, Sichuan Agricultural University, Chengdu 611130, China.

, a multifeeding insect pest, has developed a high level of resistance to chlorantraniliprole, which is a benzoylurea insecticide that targets the ryanodine receptors (RyRs). Herein, the resistant strain (SE-Sel) and sensitive strain (SE-Sus) were obtained by bidirectional screening for six generations. The potential oviposited eggs and oviposition rate of the SE-Sel strain were dramatically lower than those of the SE-Sus strain; on the contrary, the weights of prepupae and preadult were significantly increased. As a post-mating response, the higher number of non-oviposited eggs in the SE-Sel strain was caused by a lower mating rate. In addition, the expression levels of () and its receptor () in the SE-Sel strain were consistently lower than those in the SE-Sus strain. An RyR mutation, contributing to the resistance to chlorantraniliprole, was located in the S3 transmembrane segments and might have affected the release of calcium ions; it led to the upregulated expression of the neuropeptide and its receptor , and the mating and oviposition rate were significantly recovered when the was knocked down though RNA interference (RNAi) in the male adult of the SE-Sel strain. Moreover, the expression of the juvenile hormone-binding proteins and in the male adult of the SE-Sel strain was significantly decreased, which proved the existence of a fitness cost from another angle. Therefore, these results indicate that the fitness cost accompanied by chlorantraniliprole resistance in may be related to the decrease in mating desire due to overexpression.
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http://dx.doi.org/10.3390/ijms22095027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126017PMC
May 2021

Tubule-specific protein nanocages potentiate targeted renal fibrosis therapy.

J Nanobiotechnology 2021 May 26;19(1):156. Epub 2021 May 26.

Department of Pharmacology, College of Pharmacy and Laboratory Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, People's Republic of China.

Background: Despite the dramatic advances in modern medicine, efficient therapeutic measures for renal fibrosis remain limited. Celastrol (CLT) is effective in treating renal fibrosis in rat models, while causing severe systemic toxicity. Thus, we designed a tubule-specific nanocage (K3-HBc NCs) that effectively deliver CLT to tubular epithelial cell in a virus-like manner. The targeting ligand (K3) to tubular epithelial cells was displayed on the surface of Hepatitis B core protein (HBc) NCs by genetic fusion to the major immunodominant loop region. Ultra-small CLT nanodots were subtly encapsulated into the cavity through electrostatic interaction with the disassembly and reassembly of K3-HBc NCs, to yield K3-HBc/CLT complex. The efficacy of K3-HBc/CLT NCs were demonstrated in Unilateral ureteral obstruction (UUO)-induced renal fibrosis.

Results: The self-assembled K3-HBc/CLT could specifically target tubular epithelial cells via affinity with K3 ligand binding to the megalin receptor, significantly attenuating renal fibrosis. Remarkably, K3-HBc/CLT NCs significantly increased therapeutic efficacy and reduced the systemic toxicity in comparison with free CLT in UUO-induced mouse renal fibrosis model. Importantly, analysis of RNA sequencing data suggested that the anti-fibrotic effect of K3-HBc/CLT could be attributed to suppression of premature senescence in tubular epithelial cells via p21 and p16 pathway.

Conclusion: The tubule-specific K3-HBc/CLT represented a promising option to realize precise treatment for renal fibrosis.
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http://dx.doi.org/10.1186/s12951-021-00900-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157627PMC
May 2021

Fitness costs associated with chlorantraniliprole resistance in Spodoptera exigua (Lepidoptera: Noctuidae).

Pest Manag Sci 2021 Apr 14;77(4):1739-1747. Epub 2020 Dec 14.

Agricultural Insect and Pest Control Laboratory, College of Agronomy, Sichuan Agricultural University, Chengdu, China.

Background: The field population of Spodoptera exigua, an intermittently occurring polyphagous pest, has developed resistance to chlorantraniliprole, while whether or not such resistance carries fitness costs remains poorly understood. Here we selected six generations of the Leshan population (LS-P) by two-way selecting method, and obtained a highly resistant strain (CH-RE) and resistant degeneration strain (CH-SE) sharing a similar genetic background. After that fitness costs were evaluated by comparing the life history characteristics of CH-RE, CH-SE and the laboratory susceptible strain (SE-Lab) via the age-stage two-sex life table method.

Results: The resistance ratio of CH-RE and CH-SE were 226.69-fold and 3.72-fold, respectively, and the estimated realized heritability (h ) of CH-RE was 0.058. Compared with CH-SE, the duration of pre-adult, the longevity of adult, adult preoviposition period (APOP) and average generation time (T) of CH-RE had significantly increased, but the oviposition days, average fecundity, intrinsic growth rate (r), weekly growth rate (λ) and reproductive rate (R ) decreased significantly. Moreover, the relative fitness of CH-RE was 0.25, and showed fitness costs. Concurrently, the fecundity of CH-SE was slightly lower than SE-Lab, but there was no significant difference in r, λ and R , and the fitness (1) of CH-SE was similar to SE-Lab (1.02), which was no fitness cost.

Conclusion: These findings represent that chlorantraniliprole resistance in S. exigua has a fitness cost, and the fitness cost will disappear with the recovery of sensitivity when the insecticide is stopped for field populations, supporting that such resistance would be managed by switching off the selection pressure with rotation with alternate insecticides. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.6194DOI Listing
April 2021

Quercetin alleviates kidney fibrosis by reducing renal tubular epithelial cell senescence through the SIRT1/PINK1/mitophagy axis.

Life Sci 2020 Sep 20;257:118116. Epub 2020 Jul 20.

Department of Pharmacology, College of Pharmacy and Laboratory Medicine, Army Medical University (Third Military Medical University), Chongqing 400038, China. Electronic address:

Emerging evidence implicates accelerated renal tubular epithelial cell (RTEC) senescence in renal fibrosis progression. Mitophagy protects against kidney injury. However, the mechanistic interplay between cell senescence and mitophagy in RTECs is not clearly defined. The purpose of this study was to evaluate the inhibition of RTEC senescence and renal fibrosis by quercetin and explore the underlying mechanisms. We found that quercetin attenuated RTEC senescence induced by angiotensin II (AngII) in vitro and unilateral ureteral obstruction in vivo. Moreover, we demonstrated that mitochondrial abnormalities such as elevated reactive oxygen species, decreased membrane potential, and fragmentation and accumulation of mitochondrial mass, occurred in AngII-treated RTECs. Quercetin treatment reversed these effects. Furthermore, quercetin enhanced mitophagy in AngII-treated RTECs, which was markedly reduced by treatment with mitophagy-specific inhibitors. Sirtuin-1 (SIRT1) was involved in quercetin-mediated PTEN-induced kinase 1 (PINK1)/Parkin-associated mitophagy activation. Pharmacological antagonism of SIRT1 in AngII-treated RTECs blocked the effects of quercetin on mitophagy and cellular senescence. Finally, quercetin alleviated kidney fibrosis by reducing RTEC senescence via mitophagy. Collectively, the antifibrotic effect of quercetin involved inhibition of RTEC senescence, possibly through activation of SIRT1/PINK1/Parkin-mediated mitophagy. These findings suggest that pharmacological elimination of senescent cells and stimulation of mitophagy represent effective therapeutic strategies to prevent kidney fibrosis.
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http://dx.doi.org/10.1016/j.lfs.2020.118116DOI Listing
September 2020

Magnesium isoglycyrrhizinate ameliorates radiation-induced pulmonary fibrosis by inhibiting fibroblast differentiation via the p38MAPK/Akt/Nox4 pathway.

Biomed Pharmacother 2019 Jul 7;115:108955. Epub 2019 May 7.

Department of Pharmacology, College of Pharmacy and Laboratory Medicine, Army Medical University(Third Military Medical University), Chongqing, 400038, China. Electronic address:

Radiation-induced pulmonary fibrosis (RIPF) is a severe complication in patients treated with thoracic irradiation. Until now, there are no effective therapeutic drugs for RIPF. In the present study, we attempted to evaluate the effect of Magnesium isoglycyrrhizinate (MgIG) on RIPF, and to further explore the underlying mechanisms. We found that MgIG treatment markedly improved radiation-induced lung pathological changes, reduced collagen deposition, and decreased the transforming growth factor beta1 (TGF-β1) elevation induced by irradiation. In addition, MgIG treatment significantly relieved oxidative damage of pulmonary fibrosis in mice characterized by increased antioxidant factors expression and reduced oxidative factors expression. And, MgIG treatment also significantly reduced the production of intracellular reactive oxygen species (ROS) in vitro. Interestingly, administration of MgIG achieved lower expression levels of Nox4, and phosphorylation of p38MAPK and Akt in vivo and in vitro. Furthermore, treatment with MgIG notably reduced the expression levels of myofibroblast markers, Nox4, and phosphorylation of p38MAPK and Akt both in vivo and in vitro. More importantly, the inhibitory effects of MgIG on fibroblast differentiation were enhanced when the p38MAPK/Akt/Nox4 pathway was inhibited using their respective antagonists or Nox4 siRNA in vitro. Taken together, these findings suggested that MgIG could attenuate RIPF partly by inhibiting fibroblast differentiation, which was closely related to modulation of the p38MAPK/Akt/Nox4 pathway.
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http://dx.doi.org/10.1016/j.biopha.2019.108955DOI Listing
July 2019

Laboratory assays on the effects of a novel acaricide, SYP-9625 on Tetranychus cinnabarinus (Boisduval) and its natural enemy, Neoseiulus californicus (McGregor).

PLoS One 2018 5;13(11):e0199269. Epub 2018 Nov 5.

Department of Plant Protection, College of Agronomy, Sichuan Agricultural University, Chengdu, Sichuan, P. R. China.

Objective: Tetranychus cinnabarinus (Boisduval) is an agricultural mite pest threatens crops throughout the world, causing serious economic loses. Exploring the effects of acaricides on predatory mites is crucial for the combination of biological and chemical control of T. cinnabarinus. Neoseiulus californicus (McGregor) is one of the principal natural enemies of T. cinnabarinus, which can be applied in protected agriculture. In this study, the effects of sublethal concentrations of a new acaricide, SYP-9625 on two mite species, and the effects of the application concentration on predatory mite, N. californicus were assessed. The aim of the present study was to evaluate the effect of SYP-9625 on life parameters and predation capacity of N. californicus based on the concentration-response bioassay of T. cinnabarinus to explor the application of the new acaricide with natural enemy N. californicus.

Method: All of the experiments were conducted under laboratory conditions [25 ± 1°C, 16: 8 h (L: D) and 75 ± 5% RH]. The sublethal concentrations LC10 (0.375μg/mL) and the LC30 (0.841μg/mL) against T. cinnabarinus and the application concentration (100μg/mL) against N. californicus were used to evaluate the effects of SYP-9625 on population parameters of N. californicus based on an age-stage, two-sex life table and its predation capacity by functional response.

Result: cinnabarinus females treated with LC30 exhibited significantly reduced net reproductive rates (R0 = 11.02) in their offspring compared with females treated with LC10 (R0 = 14.96) and untreated females (R0 = 32.74). However, the intrinsic rate of increase (rm) and the finite rate of increase (λ) of N. californicus indicated that the application concentration of SYP-9625 had no significant negative effect on N. californicus eggs (rm = 0.277, λ = 1.319) compared to the control (rm = 0.292, λ = 1.338). Additionally, most population parameters of N. californicus showed a dose-dependent manner with the increase of the concentration of SYP-9625 against T. cinnabarinus. SYP-9625 also stimulated the control efficiency of N. californicus against immobile stages including eggs and larvae.

Conclusion: This study demonstrated that sublethal concentrations of SYP-9625 can inhibit the population growth of T. cinnabarinus. In addition, the sublethal concentrations and the application concentration showed no effect on the population growth of N. californicus. These two advantages described above showed great commercial potential of this new acaricide based on population parameters of the two mite species and predation capacity of the predatory mite under laboratory conditions.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0199269PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218021PMC
April 2019

Monitoring and biochemical characterization of beta-cypermethrin resistance in Spodoptera exigua (Lepidoptera: Noctuidae) in Sichuan Province, China.

Pestic Biochem Physiol 2018 Apr 2;146:71-79. Epub 2018 Mar 2.

Biorational Pesticide Research Lab, Sichuan Agricultural University, Chengdu 611130, China.

The beet armyworm Spodoptera exigua, a major pest affecting numerous cultivated crops in China, has developed a serious resistance to many traditional chemical insecticides. The resistance levels of the field-collected populations from different districts in Sichuan Province, China, to nine insecticides were detected with a diet-incorporation bioassay. Compared to the Lab-ZN strain, five (in 2014) and three (in 2016) field populations displayed either high or extremely high levels of resistance to beta-cypermethrin. All the field populations collected in 2014 were susceptible to emamectin benzoate, hexaflumuron, methoxyfenozide, chlorantraniliprole, cyantraniliprole and indoxacarb but exhibited low or moderate levels of resistance to abamectin. The resistances of field populations collected in 2016 were significantly higher than two years earlier, especial for chlorantraniliprole and cyantraniliprole with RRs rising from 173.4- to 582.6-fold and 175.3- to 287.6-fold, respectively, even though the field populations had retained moderate or low levels of resistance to chlorpyrifos and hexaflumuron. The synergism experiment revealed that the resistance of the LS16 population to beta-cypermethrin may be mainly related to cytochrome P450 monooxygenases (P450s), which was responsible for the highest increase ratio of 37.97-fold, for piperonyl butoxide, rather than either carboxylesterase (CarE) or glutathione S-transferase (GST). The cytochrome P450 ethoxycoumarin O-deethylase activity of the LS16 population was also the strongest among the treatments (P < 0.05). Non-denaturing polyacrylamide gel electrophoresis (native PAGE) indicated that enhanced E11, E13 and E15-E16 bands in the LS16 population likely contribute to the development of resistance to beta-cypermethrin.
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http://dx.doi.org/10.1016/j.pestbp.2018.02.008DOI Listing
April 2018

Author Correction: Grape seed proanthocyanidins prevent irradiation-induced differentiation of human lung fibroblasts by ameliorating mitochondrial dysfunction.

Sci Rep 2018 Mar 7;8(1):4389. Epub 2018 Mar 7.

Department of Pharmacology, College of Pharmacy, Third Military Medical University, Chongqing, 400038, China.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
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http://dx.doi.org/10.1038/s41598-018-22684-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5841416PMC
March 2018

Molecular identification of four novel cytochrome P450 genes related to the development of resistance of Spodoptera exigua (Lepidoptera: Noctuidae) to chlorantraniliprole.

Pest Manag Sci 2018 Aug 14;74(8):1938-1952. Epub 2018 Apr 14.

Biorational Pesticide Research Lab, College of Agriculture, Sichuan Agricultural University, Chengdu, China.

Background: The beet armyworm, Spodoptera exigua, is an omnivorous insect that damages a variety of crops worldwide. Chlorantraniliprole is a new diamide insecticide that acts on the ryanodine receptors in insects. The aim of this study was to explore key genes related to the development of chlorantraniliprole resistance in S. exigua.

Results: Transcriptomes were compared between beet armyworms from a susceptible laboratory strain (Sus-Lab) and Sus-Lab screened with LC sublethal doses of chlorantraniliprole for six generations (SE-Sel). Ten of 11 cytochrome P450 genes with upregulated expression verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in SE-Sel strains were detected in SE-PZ16 and SE-ZY16, two extremely resistant field populations. In addition, expression of four new cytochrome P450 genes, CYP9A21v1, CYP9A21v2, CYP9A21v3 and CYP9A21v4, was shown in the two field populations and was significantly higher in the SE-Sel strain than in the Sus-Lab strain (P < 0.05). Their full-length and protein tertiary structures were also cloned and predicted. The function of CYP9A21v3 was analysed by RNA interference, and the relative expression of CYP9A21v3 in the SE-ZY16 population after feeding on dsRNA was lower than in the control group. Moreover, mortality rates in insects treated at the LC of chlorantraniliprole after dsRNA feeding were significantly higher than in the control group 24 h after treatment (P < 0.05).

Conclusion: Overexpression of CYP9A21v3 may be a primary factor in the development of chlorantraniliprole resistance in beet armyworms. © 2018 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.4898DOI Listing
August 2018

Effect of PGE-EP pathway on primary cultured rat neuron injury caused by aluminum.

Oncotarget 2017 Nov 21;8(54):92004-92017. Epub 2017 Sep 21.

Department of Pharmacology, Chongqing Medical University, The Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing 400016, China.

To observe the characteristic changes of PGE-EP pathway and divergent functions of PGE receptor subtypes on neuronal injury. The primary cultured rat hippocampus neuron injury model was established via aluminum maltolate (100 μM). The aluminum-overload neurons were treated with the agonists of EP1 (17-phenyl trinor Prostaglandin E2 ethyl amide), EP2 (Butaprost), EP3 (Sulprostone) and EP4 (CAY10598) and antagonists of EP1 (SC-19220), EP2 (AH6809) and EP4 (L-161982) at different concentrations, respectively. The neuronal viability, lactate dehydrogenase leakage rate and PGE2 content were detected by MTT assay, lactate dehydrogenase assay kit and enzyme-linked immunosorbent assay, respectively. The mRNA and protein expressions of mPGES-1 and EPs were determined by RT-PCR and western blot, respectively. The pathomorphology was identified by hematoxylin-eosin staining. In the model group, neuronal viability significantly decreased, while lactate dehydrogenase leakage rate and PGE2 content increased. The mPGES-1, EP1, EP2 and EP4 mRNA expression, and the mPGES-1, EP1 and EP2 protein expression increased, while EP level decreased. EP3 agonist exerted protective function in neuronal viability and lactate dehydrogenase leakage rate, while EP1 agonist, EP2 and EP4 antagonist exerted an opposite effect. In conclusion, aluminum-overload caused an imbalance of PGE-EP pathway and activation of EP receptor may provide a viable therapeutic target in neuronal injury.
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http://dx.doi.org/10.18632/oncotarget.21122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696159PMC
November 2017

Grape seed proanthocyanidins prevent irradiation-induced differentiation of human lung fibroblasts by ameliorating mitochondrial dysfunction.

Sci Rep 2017 03 3;7(1):62. Epub 2017 Mar 3.

Department of Pharmacology, College of Pharmacy, Third Military Medical University, Chongqing, 400038, China.

Radiation-induced lung fibrosis (RILF) is a long-term adverse effect of curative radiotherapy. The accumulation of myofibroblasts in fibroblastic foci is a pivotal feature of RILF. In the study, we found the inhibitory effect of grape seed proanthocyanidins (GSPs) on irradiation-induced differentiation of human fetal lung fibroblasts (HFL1). To explore the mechanism by which GSPs inhibit fibroblast differentiation, we measured the reactive oxygen species (ROS) levels, mitochondrial function, mitochondrial dynamics, glycolysis and the signaling molecules involved in fibroblast transdifferentiation. GSPs significantly reduced the production of cellular and mitochondrial ROS after radiation. The increases in mitochondrial respiration, proton leak, mitochondrial ATP production, lactate release and glucose consumption that occurred in response to irradiation were ameliorated by GSPs. Furthermore, GSPs increased the activity of complex I and improved the mitochondrial dynamics, which were disturbed by irradiation. In addition, the elevation of phosphorylation of p38MAPK and Akt, and Nox4 expression induced by irradiation were attenuated by GSPs. Blocking Nox4 attenuated irradiation-mediated fibroblast differentiation. Taken together, these results indicate that GSPs have the ability to inhibit irradiation-induced fibroblast-to-myofibroblast differentiation by ameliorating mitochondrial dynamics and mitochondrial complex I activity, regulating mitochondrial ROS production, ATP production, lactate release, glucose consumption and thereby inhibiting p38MAPK-Akt-Nox4 pathway.
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http://dx.doi.org/10.1038/s41598-017-00108-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427826PMC
March 2017

Effect of the PGD2-DP signaling pathway on primary cultured rat hippocampal neuron injury caused by aluminum overload.

Sci Rep 2016 Apr 19;6:24646. Epub 2016 Apr 19.

Department of Pharmacology, Chongqing Medical University, the Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing 400016, China.

In the present study, the agonists and antagonists of DP receptor were used to examine whether the PGD2-DP signaling pathway affects neuronal function. Primary cultured hippocampal neuron was prepared and treated with aluminum maltolate (100 μM) to establish the neuronal damage model. PGD2 and cAMP content was detected by ELISA. L-PGDS and DPs mRNA and protein expression were measured by RT-PCR and Western blotting, respectively. The aluminium-load neuron was treated with the DP1 agonist BW245C, the DP1 antagonist BWA868C, the DP2 agonist DK-PGD2, and the DP2 antagonist CAY10471, respectively. Neuronal pathomorphology was observed using H-E staining. The cell viability and the lactate dehydrogenase leakage rates of neurons were measured with MTT and LDH kit, respectively. Ca(2+) level was detected by Fluo-3/AM. In the model group, the MTT values obviously decreased; LDH leakage rates and PGD2 content increased significantly; L-PGDS, DP1 mRNA and protein expressions increased, and DP2 level decreased. BW245C reduced the Ca(2+) fluorescence intensity and protected the neurons. DK-PGD2 increased the intensity of Ca(2+) fluorescence, while CAY10471 had the opposite effect. In conclusion, contrary to the effect of DP2, the PGD2-DP1 signaling pathway protects against the primary cultured rat hippocampal neuronal injury caused by aluminum overload.
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http://dx.doi.org/10.1038/srep24646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835855PMC
April 2016

Direct Effects of Elevated CO2 Levels on the Fitness Performance of Asian Corn Borer (Lepidoptera: Crambidae) for Multigenerations.

Environ Entomol 2015 Aug 6;44(4):1250-7. Epub 2015 Jul 6.

The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China.

Understanding direct response of insects to elevated CO2 should help to elucidate the mechanistic bases of the effects of elevated CO2 on interactions of insects with plants. This should improve our ability to predict shifts in insect population dynamics and community interactions under the conditions of climate change. Effects of elevated CO2 levels on the fitness-related parameters were examined for multigenerations in the Asian corn borer, Ostrinia furnacalis (Guenée). The larvae were allowed to feed on artificial diet, and reared in the closed-dynamic environment chambers with three CO2 levels (ambient, 550 μl/liter, and 750 μl/liter) for six generations. In comparison with the ambient CO2 level, mean larval survival rate decreased 9.9% in 750 μl/liter CO2 level, across O. furnacalis generations, and larval and pupal development times increased 7.5-16.4% and 4.5-13.4%, respectively, in two elevated CO2 levels. Pupal weight was reduced more than 12.2% in 750 μl/liter CO2 level. Across O. furnacalis generations, mean food consumption per larva increased 2.7, 7.0% and frass excretion per larva increased 14.4, 22.5% in the two elevated CO2 levels, respectively, compared with ambient CO2 level. Elevated CO2 levels resulted in the decline mean across O. furnacalis generations in mean relative growth rate, but increased in relative consumption rate. These results suggested that elevated CO2 would reduce the fitness-related parameters such as higher mortality, lower pupal weight, and longer development times in long term. It also reduced the larval food digestibility and utilizing efficiency; in turn, this would result in increase of food consumption.
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http://dx.doi.org/10.1093/ee/nvv102DOI Listing
August 2015

The Effect of Chemical Composition and Bioactivity of Several Essential Oils on Tenebrio molitor (Coleoptera: Tenebrionidae).

J Insect Sci 2015 7;15. Epub 2015 Aug 7.

Sichuan Agricultural University, Biorational Pesticide Research Lab, 611130, Chengdu, Republic of China.

The major chemical components of four essential oils (EOs) extracted from dry leaves of Citrus limonum, Cymbopogon citratus, Litsea cubeba, and Muristica fragrans were analyzed with gas chromatograph-mass spectrometer and their fumigant, contact, and repellent activities against 10th instar and adults of Tenebrio molitor were also assayed. The results indicated that the major constituents of C. limonum and Cy. citrates were D-limonene (38.22%) and 3,7-dimethyl-6-octenal (26.21%), while which of L. cubeba and M. fragrans were (E)-3, 7-dimethyl-2, 6-octadienal (49.78%) and (E)-cinnamaldehyde (79.31%), respectively. Contact activities of L. cubeba and C. limonum with LC50 values of 21.2 and 13.9 µg/cm(2) at 48 h and repellence activities (>89.0% repellence indexes) (P < 0.05) at 12 h on 10th instar were better than those of the other two EOs. Nevertheless, the fumigation activities of L. cubeba on 10th instar and adults (LC50 = 2.7, 3.7 μl/liter) were stronger than those of C. limonum (LC50 = 10.9, 12.0 μl/liter) at 96 h and significant (not overlapping confidence intervals). The EOs of L. cubeba and C. limonum have clearly elongated the growth and development of larvae, egg, and slightly shorten pupae and adults of T. molitor compared with the control. The mainly active ingredients of L. cubeba and C. limonum, including D-limonene and β-pinene, were demonstrated to coinhibit the actives of AChE and enhance the toxicities on 10th instar of T. molitor. These results indicate that the EOs of L. cubeba and C. limonum could have great potential as botanical insecticides against T. molitor.
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http://dx.doi.org/10.1093/jisesa/iev093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4672209PMC
April 2016

Effects of atorvastatin on warfarin-induced aortic medial calcification and systolic blood pressure in rats.

J Huazhong Univ Sci Technolog Med Sci 2008 Oct 10;28(5):535-8. Epub 2008 Oct 10.

Department of Geriatrics, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

The effect of atorvastatin on warfarin-induced aortic medial calcification and systolic blood pressure (SBP) of rats induced by warfarin was studied. Thirty healthy and adult rats were randomly divided into Warfarin group (n=10), Atorvastatin group (n=10) and normal control group (n=10). Caudal arterial pressure of rats was measured once a week, and 4 weeks later, aorta was obtained. Elastic fiber, collagen fiber and calcium accumulation in tunica media of cells were measured by Von Kossa staining. The results showed that warfarin treatment led to elevation of systolic blood pressure and aortic medial calcification. The chronic treatment also increased collagen, but decreased elastin in the aorta. However, the atorvastatin treatment had adverse effects. It was concluded that treatment with atorvastatin presented evidence of blood pressure lowing and calcification reducing. These data demonstrate that atorvastatin protected aortic media from warfarin-induced calcification and elevation of systolic blood pressure.
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http://dx.doi.org/10.1007/s11596-008-0510-1DOI Listing
October 2008
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