Publications by authors named "Quan Wang"

698 Publications

Liquefied dimethyl ether based multi-stage extraction for high efficient oil recovery from spent bleaching clay.

Waste Manag 2021 Oct 23;136:204-212. Epub 2021 Oct 23.

Civil College, Nanjing Forest University, No.159, Longpan Road, Nanjing 210037, China.

With the increasing production of spent bleaching clay (SBC), the recovery of the waste oil in SBC is becoming an important and urgent needs for our environment and economy. In this research, we have developed a new effective recovery technique to recover oil from SBC by use of liquefied dimethyl ether (DME). Over 65 wt% oil and 81% wt.% oil are efficiently recovered from SBC under equilibrium single-stage extraction conditions and multi-stage extraction conditions, respectively based on the systematically investigation to the effects of the DME/SBC ratio, extraction time, stirring speed and extraction stage number on oil recovery via a batch extractor. Compared with using other extraction solvents, the extraction solvent DME can be reused without heating and therefore significantly reduce the energy consumption during the oil recovery process. In addition, the quantitative oil extraction relationship is derived from the adsorption equilibrium model and is well verified by experimental results. The results show a great potential for using this oil recovery technique in SBC as well as in the large amount of oily sludge and oil sands.
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http://dx.doi.org/10.1016/j.wasman.2021.09.033DOI Listing
October 2021

Enzyme-Activatable Aggregation-Induced Emission Probe: Intraoperative-Pathological Fluorescent Diagnosis of Pancreatic Cancer via Specific Cathepsin E.

Adv Mater 2021 Oct 25:e2107444. Epub 2021 Oct 25.

Shanghai Key Laboratory of Functional Materials Chemistry, Key Laboratory for Advanced Materials and Institute of Fine Chemicals, Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China.

Pancreatic cancer (PC) is one of the most devastating malignant tumors. However, fluorescence probes for early clinical diagnosis of PC often encounter difficulties in the accuracy and penetrability. Herein, we develop an enzyme activatied aggregation-induced emission (AIE) probe QM-HSP-CPP for high-contrast fluorescence diagnosis of PC by monitoring specific overexpressed enzyme Cathepsin E (CTSE). The probe is composed of an AIE fluorophore QM-COOH, CTSE-triggered hydrophobic peptide (HSP), and hydrophilic biocompatible cell penetrating peptide (CPP). The CPP unit could well modulate the molecular dispersion properties, giving the initial fluorescence-off state in the aqueous biosystem, thus endowing high signal-to-noise ratio, and finially overcoming the poor targeting selectivity of traditional AIE probes. CPP can ensure cell/tissue penetrating ability, thus allowing on-site monitoring endogenous CTSE in PC cells, tissues, and living animal models. When the probe QM-HSP-CPP is specifically cleaved by CTSE, it could generate AIE signals in situ with high-specificity and long-term tracking ability, and successfully achieve the intraoperatively diagnosis of human PC sections and tracking PC in heterotopic nude mice models. The CTSE enzyme-triggered AIEgens liberation strategy improves the accuracy and addresses the penetration problem simultaneously, which can expand the database of multitudinous biocompatible AIE-active probes, especially for establishing the intraoperative-pathological fluorescent diagnosis. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202107444DOI Listing
October 2021

Red blood cell membrane-camouflaged nanoparticles loaded with AIEgen and Poly(I : C) for enhanced tumoral photodynamic-immunotherapy.

Natl Sci Rev 2021 Jun 3;8(6):nwab039. Epub 2021 Mar 3.

Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.

Red blood cell (RBC)-mimicking nanoparticles (NPs) offer a promising platform for drug delivery because of their prolonged circulation time, reduced immunogenicity and specific targeting ability. Herein, we report the design and preparation of RBC membrane-bound NPs ([email protected]), for tumoral photodynamic-immunotherapy. The [email protected] is formed by self-assembly of the positively charged aggregation-induced emission luminogen (AIEgen) (named P2-PPh3) and the negatively charged polyinosinic : polycytidylic acid (Poly(I : C)), followed by RBC membrane encapsulation. P2-PPh3 is an AIE-active conjugated polyelectrolyte with additional photosensitizing ability for photodynamic therapy (PDT), while Poly(I : C) serves as an immune-stimulant to stimulate both tumor and immune cells to activate immunity, and thus reduces tumor cell viability. When applied in tumor-bearing mice, the [email protected] NPs are enriched in both the tumor region as a result of an enhanced permeability and retention (EPR) effect, and the spleen because of the homing effect of the RBC-mimicking shell. Upon light irradiation, P2-PPh3 promotes strong ROS generation in tumor cells, inducing the release of tumor antigens (TA). The anti-tumor immunity is further enhanced by the presence of Poly(I : C) in [email protected] Thus, this strategy combines the PDT properties of the AIE-active polyelectrolyte and immunotherapy properties of Poly(I : C) to achieve synergistic activation of the immune system for anti-tumor activity, providing a novel strategy for tumor treatment.
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http://dx.doi.org/10.1093/nsr/nwab039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288176PMC
June 2021

Remdesivir overcomes the S861 roadblock in SARS-CoV-2 polymerase elongation complex.

Cell Rep 2021 Oct 8:109882. Epub 2021 Oct 8.

Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, No. 44 Xiao Hong Shan, Wuhan, Hubei 430071, China; Drug Discovery Center for Infectious Diseases, Nankai University, Tianjin 300350, China. Electronic address:

Remdesivir (RDV), a nucleotide analog with broad-spectrum features, has exhibited effectiveness in COVID-19 treatment. However, the precise working mechanism of RDV when targeting the viral RNA-dependent RNA polymerase (RdRP) has not been fully elucidated. Here, we solve a 3.0-Å structure of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RdRP elongation complex (EC) and assess RDV intervention in polymerase elongation phase. Although RDV could induce an "i+3" delayed termination in meta-stable complexes, only pausing and subsequent elongation are observed in the EC. A comparative investigation using an enterovirus RdRP further confirms similar delayed intervention and demonstrates that steric hindrance of the RDV-characteristic 1'-cyano at the -4 position is responsible for the "i+3" intervention, although two representative Flaviviridae RdRPs do not exhibit similar behavior. A comparison of representative viral RdRP catalytic complex structures indicates that the product RNA backbone encounters highly conserved structural elements, highlighting the broad-spectrum intervention potential of 1'-modified nucleotide analogs in anti-RNA virus drug development.
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http://dx.doi.org/10.1016/j.celrep.2021.109882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498683PMC
October 2021

Assessment of Clinicopathological Characteristics and Development of an Individualized Prognostic Model for Patients With Hepatoid Adenocarcinoma of the Stomach.

JAMA Netw Open 2021 Oct 1;4(10):e2128217. Epub 2021 Oct 1.

Department of Gastric Surgery, Fujian Medical University Union Hospital, Fuzhou, China.

Importance: Few studies have examined the clinicopathological characteristics and prognoses of patients with hepatoid adenocarcinoma of the stomach (HAS).

Objective: To explore the clinicopathological characteristics and prognoses of patients with HAS and develop a nomogram to predict overall survival (OS).

Design, Setting, And Participants: This prognostic study involved a retrospective analysis of data from 315 patients who received a diagnosis of primary HAS between April 1, 2004, and December 31, 2019, at 14 centers in China.

Main Outcomes And Measures: OS and prognostic factors. Patients were randomly assigned to a derivation cohort (n = 220) and a validation cohort (n = 95). A nomogram was developed based on independent prognostic factors identified through a multivariable Cox mixed-effects model.

Results: Among 315 patients with HAS (mean [SD] age, 61.9 [10.2] years; 240 men [76.2%]), 137 patients had simple HAS (defined as the presence of histologically contained hepatoid differentiation areas only), and 178 patients had mixed HAS (defined as the presence of hepatoid differentiation areas plus common adenocarcinoma areas). Patients with simple HAS had a higher median preoperative α-fetoprotein level than those with mixed HAS (195.9 ng/mL vs 48.9 ng/mL, respectively; P < .001) and a higher rate of preoperative liver metastasis (23 of 137 patients [16.8%] vs 11 of 178 patients [6.2%]; P = .003). The 3-year OS rates of patients with simple vs mixed HAS were comparable (56.0% vs 60.0%; log-rank P = .98). A multivariable Cox analysis of the derivation cohort found that the presence of perineural invasion (hazard ratio [HR], 2.13; 95% CI, 1.27-3.55; P = .009), preoperative carcinoembryonic antigen levels of 5 ng/mL or greater (HR, 1.72; 95% CI, 1.08-2.74; P = .03), and pathological node category 3b (HR, 3.72; 95% CI, 1.34-10.32; P = .01) were independent risk factors for worse OS. Based on these factors, a nomogram to predict postoperative OS was developed. The concordance indices of the nomogram (derivation cohort: 0.72 [95% CI, 0.66-0.78]; validation cohort: 0.72 [95% CI, 0.63-0.81]; whole cohort: 0.71 [95% CI, 0.66-0.76]) were higher than those derived using the American Joint Committee on Cancer's AJCC Cancer Staging Manual (8th edition) pathological tumor-node-metastasis (pTNM) staging system (derivation cohort: 0.63 [95% CI, 0.57-0.69]; validation cohort: 0.65 [95% CI, 0.56-0.75]; whole cohort: 0.64 [95% CI, 0.59-0.69]) and those derived using a clinical model that included pTNM stage and receipt of adjuvant chemotherapy (derivation cohort: 0.64 [95% CI, 0.58-0.69]; validation cohort: 0.65 [95% CI, 0.56-0.75]; whole cohort: 0.64 [95% CI, 0.59-0.69]). Based on the nomogram cutoff of 10 points, the whole cohort was divided into high-risk and low-risk groups. The 3-year OS rate of patients in the high-risk group was significantly lower than that of patients in the low-risk group (29.7% vs 75.9%, respectively; log-rank P < .001), and the 3-year prognosis of high-risk and low-risk groups could be further distinguished into pTNM stage I to II (33.3% vs 80.2%; exact log-rank P = .15), stage III (34.3% vs 71.3%; log-rank P < .001), and stage IV (15.5% vs 70.3%; log-rank P = .009).

Conclusions And Relevance: This study found that perineural invasion, preoperative carcinoembryonic antigen levels of 5 ng/mL or greater, and pathological node category 3b were independent risk factors associated with worse OS. An individualized nomogram was developed to predict OS among patients with HAS. This nomogram had good prognostic value and may be useful as a supplement to the current American Joint Committee on Cancer TNM staging system.
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http://dx.doi.org/10.1001/jamanetworkopen.2021.28217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8493440PMC
October 2021

Zhike Pingchuan Granule suppresses interleukin (IL)-6 or the medium of M2 macrophages induced apoptosis in human bronchial epithelial cells.

Bioengineered 2021 Dec;12(1):7694-7703

Pediatric Department, The Second Clinical Medical College of Henan University of Traditional Chinese Medicine, Zhengzhou, China.

The aim of this study was to explore the effects and action mechanism of Zhike Pingchuan Granule in human bronchial epithelial cells induced by IL-6 or the supernatant of M2. Upon IL-6 stimulation at different doses, Cell Counting Kit-8 (CCK8) assay and flow cytometry were, respectively, utilized to detect the cell viability and apoptosis levels of 16-HBE cells. ELISA and Western blot were, respectively, used to analyze the inflammatory markers and JAK2/STAT3 signals. Immunofluorescence assay was performed to identify M0 and M2 cells. As shown in results, ZKPC perturbed the expression of IL-6 inducible genes important for apoptosis, oxidative and inflammatory response, which was enhanced by JAK2 inhibitor. Besides the inhibitory effects on the phosphorylation levels of JAK2/STAT3, ZKPC markedly increased cell viability and reduced apoptosis in human bronchial epithelial cells (16-HBE) cultured in the supernatant of M2 cells. Collectively, ZKPC could inhibit the IL-6-induced JAK/STAT3 signaling cascade, increase cell viability and decrease apoptosis induced by the supernatant of M2. A more comprehensive understanding of the action mechanism of ZKPC on JAK2/STAT3 signaling pathway in human bronchial epithelial cells induced by IL-6 or M2 supernatant will enable ZKPC development in the control of asthma.
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http://dx.doi.org/10.1080/21655979.2021.1982309DOI Listing
December 2021

Is Early Controlled Motion and Weightbearing Recommended for Nonoperatively Treated Acute Achilles Tendon Rupture? A Systematic Review and Meta-analysis.

Orthop J Sports Med 2021 Sep 21;9(9):23259671211024605. Epub 2021 Sep 21.

Department of Orthopedics, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, PR China.

Background: There is disagreement as to whether early controlled motion and weightbearing confer a beneficial effect for nonoperatively treated acute Achilles tendon rupture (ATR) compared with immobilization and late weightbearing.

Purpose: To conduct a meta-analysis of randomized controlled trials (RCTs) to determine whether early controlled motion and weightbearing results in different outcomes compared with immobilization and late weightbearing for nonoperatively treated patients with acute ATR.

Study Design: Systematic review; Level of evidence, 1.

Methods: We conducted a search in the PubMed, Web of Science, and EMBASE databases for relevant RCTs in humans from January 1981 to August 2020. The primary outcome was the Achilles Tendon Total Rupture Score (ATRS) at 1-year follow-up. The secondary outcomes were the rerupture rate, return to sports activity and work, and the heel-rise work (limb symmetry index [LSI]). Study quality was assessed using the Cochrane Collaboration risk of bias tool.

Results: Included were 7 RCTs involving 424 participants (n = 215 treated with early controlled motion and weightbearing [early group], n = 209 treated with immobilization and late weightbearing [late group]). The quality assessment indicated a low risk of bias in all included RCTs. There was no difference between the early and late groups regarding the ATRS (mean difference [MD], -0.220; 95% CI, -4.489 to 4.049; = .920). Likewise, we found no difference between the 2 groups in terms of the rerupture rate (odds ratio [OR], 1.107; 95% CI, 0.552 to 2.219; = .775), the number of patients who returned to sports (OR, 0.766; 95% CI, 0.438 to 1.341; = .351) and returned to work (OR, 0.706; 95% CI, 0.397 to 1.253; = .234), the time to return to work (MD, -2.802 days; 95% CI, -6.525 to 0.921 days; = .140), or the heel-rise work LSI (MD, -0.135; 95% CI, -6.243 to 5.973; = .965).

Conclusion: No significant differences were found between early controlled motion and weightbearing compared with immobilization and late weightbearing regarding the ATRS, the rerupture rate, return to sports activity and work, and the heel-rise work in nonoperatively treated patients with acute ATR.
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http://dx.doi.org/10.1177/23259671211024605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461133PMC
September 2021

Memory Deficit in Patients With Temporal Lobe Epilepsy: Evidence From Eye Tracking Technology.

Front Neurosci 2021 7;15:716476. Epub 2021 Sep 7.

Key Laboratory of Spectral Imaging Technology, Xi'an Institute of Optics and Precision Mechanics of the Chinese Academy of Sciences, Xi'an, China.

To explore quantitative measurements of the visual attention and neuroelectrophysiological relevance of memory deficits in temporal lobe epilepsy (TLE) by eye tracking and electroencephalography (EEG). Thirty-four TLE patients and twenty-eight healthy controls were invited to complete neurobehavioral assessments, cognitive oculomotor tasks, and 24-h video EEG (VEEG) recordings using an automated computer-based memory assessment platform with an eye tracker. Visit counts, visit time, and time of first fixation on areas of interest (AOIs) were recorded and analyzed in combination with interictal epileptic discharge (IED) characteristics from the bilateral temporal lobes. The TLE patients had significantly worse Wechsler Digit Span scores [(1, 58) = 7.49, = 0.008]. In the Short-Term Memory Game with eye tracking, TLE patients took a longer time to find the memorized items [(1, 57) = 17.30, < 0.001]. They had longer first fixation [(1, 57) = 4.06, = 0.049] and more visit counts [(1, 57) = 7.58, = 0.008] on the target during the recall. Furthermore, the performance of the patients in the Digit Span task was negatively correlated with the total number of IEDs [(28) = -0.463, = 0.013] and the number of spikes per sleep cycle [(28) = -0.420, = 0.026]. Eye tracking appears to be a quantitative, objective measure of memory evaluation, demonstrating memory retrieval deficits but preserved visual attention in TLE patients. Nocturnal temporal lobe IEDs are closely associated with memory performance, which might be the electrophysiological mechanism for memory impairment in TLE.
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http://dx.doi.org/10.3389/fnins.2021.716476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453169PMC
September 2021

Photosynthetic physiological response of water-saving and drought-resistant rice to severe drought under wetting-drying alternation irrigation.

Physiol Plant 2021 Sep 22. Epub 2021 Sep 22.

Agricultural College, Anhui Agricultural University, Hefei, Anhui, China.

Water-saving and drought-resistant rice (WDR) is widely grown in central China in recent years. However, studies have not explored the interaction effect of WDR and irrigation regimes on drought-resistance capacities under severe drought at sensitive growth periods. A pot experiment was conducted using a WDR cultivar Hanyou73 (HY73) and traditional high-yielding and drought-sensitive cultivar Huiliangyou 898 (HLY898). Three irrigation regimes, including flooding irrigation (W1), mild wetting-drying alternation irrigation (W2), and severe wetting-drying alternation irrigation (W3), were applied before heading. At heading, severe drought with -50 KPa soil water potential was established for all treatments and cultivars. The findings showed that cultivar HY73 under W2 treatment had the highest yield, 1000-grain yield, filled grain, relative water content, and photosynthesis potential compared with the other combinations. The higher net photosynthetic rate (P ) was attributed to larger mesophyll conductance (g ) in drought for cultivar HY73 under W2 treatment compared with that for cultivar HLY898 and the other water treatments. Enhanced photo-respiration rate may be an important photoprotection mechanism for achieving high P for cultivar HY73 coupled with W2 treatment than for other combinations in drought. The relative expression level of OsPIP1;1 gene was significantly down-regulated during drought in all cultivars and water regimes. But OsPIP1;2, OsPIP2;3, OsTIP2;2, and OsTIP3;1 genes were upregulated to alleviate the significant decrease in g and g under drought. These results suggest that WDR and mild wetting-drying alternation irrigation (W2) have significant interaction effects in improving photosynthetic production potential by maintaining higher g under severe drought.
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http://dx.doi.org/10.1111/ppl.13568DOI Listing
September 2021

Increased dielectric performance of PVDF-based composites by electrochemical exfoliated graphite additives.

Nanotechnology 2021 Oct 6;32(50). Epub 2021 Oct 6.

Zhenjiang Key Laboratory of Advanced Sensing Materials and Devices, Jiangsu University, Zhenjiang 212013, People's Republic of China.

Adding conductive graphene into polyvinyliden1 fluoride (PVDF) is an effective method to improve the dielectric properties. However, the high conductivity and uniform distribution of graphene in PVDF matrix still meet challenges. In this work, electrochemical exfoliated graphite (EEG) with good conductivity and solution dispersion is used to prepare PVDF/EEG films. By this method no specific conditions are needed such as reduction or hot press. The dielectric properties and flexibility of PVDF/EEG films of different concentrations of EEG are investigated. Especially, when EEG content is 2.6 wt%, composite film has a high dielectric constant of 86 and a low dielectric loss of 0.9 (100 Hz). Simultaneously, the tensile strength of the film was up to 55.1 MPa. The preparation method is simple and convenient, and the obtained material has high dielectric constant and tensile strength. This method paves the way for the application of flexible electronic sensing equipment.
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http://dx.doi.org/10.1088/1361-6528/ac28d9DOI Listing
October 2021

TelecomNet: Tag-based Weakly-supervised Modally Cooperative Hashing Network for Image Retrieval.

IEEE Trans Pattern Anal Mach Intell 2021 Sep 21;PP. Epub 2021 Sep 21.

We are concerned with using user-tagged images to learn proper hashing functions for image retrieval. The benefits are two-fold: (1) we could obtain abundant training data for deep hashing models; (2) tagging data possesses richer semantic information which could help better characterize similarity relationships between images. However, tagging data suffers from noises, vagueness and incompleteness. Different from previous unsupervised or supervised hashing learning, we propose a novel weakly-supervised deep hashing framework which consists of two stages: weakly-supervised pre-training and supervised fine-tuning. The second stage is as usual. In the first stage, we propose two formulations Tag-basEd weakLy-supErvised Modally COoperative hashing Network (TelecomNet) and Generalized TelecomNet (GTelecomNet). Rather than performing supervision on tags, TelecomNet first learns an observed semantic embedding vector for each image from attached tags and then uses it to guide hashing learning. GTelecomNet introduces a novel semantic network to exploit more precise semantic information. By carefully designing the optimization problem, they can well leverage tagging information and image content for hashing learning. The framework is general and does not depend on specific deep hashing methods. Empirical results on real world datasets show that they significantly increase the performance of state-of-the-art deep hashing methods.
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http://dx.doi.org/10.1109/TPAMI.2021.3114089DOI Listing
September 2021

White Matter but not Gray Matter Volumes are Associated with Cognition in Community-Dwelling Chinese Populations.

J Alzheimers Dis 2021 Sep 16. Epub 2021 Sep 16.

Department of Neurology , State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Few studies have investigated the association between cognition and brain volume associated with cerebral small vessel disease (CSVD).

Objective: We investigated the association between cognition and brain volume and neuroimaging markers of CSVD in a community-dwelling population.

Methods: Participants (n = 993, age≥35 years) from the community-based Shunyi Study were included to investigate the association between neuroimaging markers and cognition cross-sectionally. Magnetic resonance imaging markers included brain volume measurements of the total cerebrum, white matter, gray matter, and CSVD imaging markers. Cognitive performance was assessed using neuropsychological tests of the Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), Fuld Object Memory, digit span, Trail Making Test (TMT)-A, and TMT-B.

Results: For brain volume measurement, subcortical white matter fraction was positively associated with MMSE score (β= 0.034, p = 0.0062) and MoCA score (β= 0.034, p = 0.0174), and negatively associated with TMT-A and TMT-B completion time (β= -2.319, p = 0.0002; β= -2.827, p = 0.0073, respectively). For evaluation of CSVD imaging markers, the presence of lacunes was positively associated with TMT-B completion time (β= 17.241, p = 0.0028).

Conclusion: In community-dwelling populations, reduced white matter volumes, as a consequence of aging and vascular damage, are associated with worse global cognition and executive function. Our findings provide potential insights into the correlation between cognition and CSVD-associated subcortical white matter injury.
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http://dx.doi.org/10.3233/JAD-210367DOI Listing
September 2021

Genetic mutations associated with sensitivity to neoadjuvant chemotherapy in metastatic colon cancer: A case report and review of literature.

World J Clin Cases 2021 Aug;9(24):7099-7109

Department of Gastroenterological Surgery, Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, Beijing 100044, China.

Background: Colorectal liver metastases (CLM) occur in 15%-30% of patients with colorectal cancer (CRC). Advancements in next generation sequencing (NGS) can provide more precise prognoses for cancer patients and help guide clinical treatment. However, the genetic variants that predict high sensitivity to neoadjuvant chemotherapy remain unclear, especially in patients with CLM. The aim of this study was to identify the relevant genetic variants in a single CLM patient and to summarize the current evidence on mutations and single nucleotide polymorphisms (SNPs) that objectively predict sensitivity to neoadjuvant chemotherapy.

Case Summary: A 76-year-old male patient, who was diagnosed as stage IV colon cancer with liver metastases, was found to have mutations. He showed a good therapeutic response to 12 courses of oxaliplatin regimens combined with Bevacizumab. Genetic analysis of the patient identified 5 genes with 7 detected SNPs that may be related to a better response to chemotherapy drugs. In addition, a critical literature review was performed based on a standardized appraisal form after selecting the articles. Ultimately, 21 eligible studies were appraised to assess the association between gene mutations and good prognosis. Mutations in and were identified as being associated with a poor response to chemotherapy drugs, whereas mutations of and were associated with longer overall survival.

Conclusion: NGS can identify precise predictors of response to neoadjuvant chemotherapy, leading to improved outcomes for CRC patients.
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http://dx.doi.org/10.12998/wjcc.v9.i24.7099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409210PMC
August 2021

Effects of microplastics on humification and fungal community during cow manure composting.

Sci Total Environ 2021 Sep 1;803:150029. Epub 2021 Sep 1.

College of Natural Resources and Environment, Northwest A&F University, Yangling 712100, China; Key Laboratory of Plant Nutrition and the Agrienvironment in Northwest China, Ministry of Agriculture, Yangling, 712100, Shaanxi, China. Electronic address:

The effect of microplastics (MPs) on the biological treatment of organic waste has been extensively studied, but little is known about the influence of different MPs on composting humification and the fungal community. In this study, PE, PVC, and PHA MPs were individually mixed with cow dung and sawdust and then composted. The results showed that different MPs had various influences on humification, and the humic acid to fulvic acid ratio of all MP-added treatments (0.44-0.83) was lower than that of the control (0.91). During the composting process, Ascomycota (26.32-89.14%) and Basidiomycota (0.47-4.78%) are the dominant phyla in all treatments and all microplastics decreased the diversity and richness of the fungal community at the thermophilic stage of composting. Exposure to MPs had an obvious effect on the fungal community at the genus level, and the addition of PHA and PE MPs increased the relative abundance of phytopathogenic fungi. LEfSe and network analysis indicated that MPs reduced the number of biomarkers and led to a simpler and more unstable fungal community structure compared to the control. This study has important implications for assessing microplastic pollution and organic waste disposal.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150029DOI Listing
September 2021

Zirconium hydroxide nanoparticle encapsulated magnetic biochar composite derived from rice residue: Application for As(III) and As(V) polluted water purification.

J Hazard Mater 2021 Sep 4;423(Pt A):127081. Epub 2021 Sep 4.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China; Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi Province 712100, China.

Finding a low-cost and suitable adsorbent is still in urgent need for efficient decontamination of As(III) and As(V) elements from the polluted waters. A novel zirconium hydroxide nanoparticle encapsulated magnetic biochar composite (ZBC) derived from rice residue was synthesized for the adsorptive capture of As(III) and As(V) from aqueous solutions. The results revealed that ZBC showed an acceptable magnet separation ability and its surface was encapsulated with lots of hydrous zirconium oxide nanoparticles. Compared to As(III), the adsorption of As(V) onto ZBC was mainly dependent on the pH of the solution. The intraparticle diffusion model described the adsorption process. ZBC showed satisfactory adsorption performances to As(III) and As(V) with the highest adsorption quantity of 107.6 mg/g and 40.8 mg/g at pH 6.5 and 8.5, respectively. The adsorption of As(III) and As(V) on ZBC was almost impervious with the ionic strength while the presence of coexisting ions, especially phosphate, significantly affected the adsorption process. The processes of complexation reaction and electrostatic attraction contributed to the adsorption of As(III) and As(V) onto ZBC. ZBC prepared from kitchen rice residue was found to be a low cost environmentally friendly promising adsorbent with high removal capacity for As(III) and As(V) and could be recycled easily from contaminated waters.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127081DOI Listing
September 2021

Work productivity loss in breast cancer survivors and its effects on quality of life.

Work 2021 ;70(1):199-207

Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.

Background: Return to work is an important process for many breast cancer survivors (BCSs) that acts as a positive step towards their reintegration into society.

Objectives: This study examined whether work productivity loss due to presenteeism could predict the quality of life (QOL) of employed BCSs.

Methods: This study used a cross-sectional design. Seventy-five BCSs and seventy-five participants in the Non-Cancer Comparison (NCC) group were surveyed. The main outcome measures were productivity loss (as measured by the Work Limitations questionnaire) and quality of life (as measure by the European Organization for Research and Treatment Quality of Life questionnaire C30). Other measures included psychological distress (as measured by the Hospital Anxiety and Depression Scale) and cognitive limitation at work (as measured by the Cognitive Symptom Checklist).

Results: The BCS group had a lower summary score, a lower global health related score and greater work limitation in all domains than the NCC group. The productivity loss due to presenteeism of the BCS group was 8%. The multiple regression model shows that work productivity loss and level of job stress were the significant predictors of quality of life in the BCS group.

Conclusion: These findings raise questions about the effects of level of job stress and work productivity loss on the QOL of BCSs. Longitudinal studies are needed to map these relationships.
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http://dx.doi.org/10.3233/WOR-213565DOI Listing
October 2021

Expert consensus of perioperative intensive care and management of critically ill cancer patients (2021).

Ann Palliat Med 2021 09 4;10(9):9331-9341. Epub 2021 Sep 4.

Department of Intensive Care Unit, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

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http://dx.doi.org/10.21037/apm-21-870DOI Listing
September 2021

Selecting informative bands for partial least squares regressions improves their goodness-of-fits to estimate leaf photosynthetic parameters from hyperspectral data.

Photosynth Res 2021 Sep 7. Epub 2021 Sep 7.

Graduate School of Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan.

The plant photosynthetic capacity determines the photosynthetic rates of the terrestrial biosphere. Timely approaches to obtain the spatiotemporal variations of the photosynthetic parameters are urgently needed to grasp the gas exchange rhythms of the terrestrial biosphere. While partial least squares regression (PLSR) is a promising way to predict the photosynthetic parameters maximum carboxylation rate (V) and maximum electron transport rate (J) rapidly and non-destructively from hyperspectral data, the approach, however, faces a high risk of overfitting and remains a high hurdle for applications. In this study, we propose to incorporate proper band selection techniques for PLSR analysis to refine the goodness-of-fit (GoF) in estimating V and J. Different band selection procedures coupled with different hyperspectral forms (reflectance, apparent absorption, as well as derivatives) were examined. Our results demonstrate that the GoFs of PLSR models could be greatly improved by combining proper band selection methods (especially the iterative stepwise elimination approach) rather than using full bands as commonly done with PLSR. The results also show that the 1st order derivative spectra had a balance between accuracy (R = 0.80 for V, and 0.94 for J) and denoising (when a Gaussian noise was added to each leaf reflectance spectrum at each wavelength with a standard deviation of 1%) on retrieving photosynthetic parameters from hyperspectral data. Our results clearly illustrate the advantage of using the band selection approach for PLSR dimensionality reduction and model optimization, highlighting the superiority of using derivative spectra for V and J estimations, which should provide valuable insights for retrieving photosynthetic parameters from hyperspectral remotely sensed data.
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http://dx.doi.org/10.1007/s11120-021-00873-9DOI Listing
September 2021

Prevalence and Molecular Characteristics Based on Whole Genome Sequencing of Resistant to Four Anti-Tuberculosis Drugs from Southern Xinjiang, China.

Infect Drug Resist 2021 24;14:3379-3391. Epub 2021 Aug 24.

State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, People's Republic of China.

Objective: Drug-resistant tuberculosis is a major public health problem, especially in the southern region of Xinjiang, China; however, there is little information regarding drug resistance profiles and mechanism of in this area. The aim of this study was to determine the prevalence and molecular characteristics of resistant to four anti-tuberculosis drugs from this area.

Methods: Three hundred and forty-six isolates from the southern region of Xinjiang, China were included and used to perform phenotypic drug susceptibility testing and whole genome sequencing (WGS). Mutations in seven loci associated with drug resistance, including for rifampicin (RMP), promoter and for isoniazid (INH), 530 and 912 loops and for streptomycin (STR), and for ethambutol (EMB), were characterized.

Results: Among 346 isolates, 106, 60, 70 and 29 were resistant to INH, RMP, STR and EMB, respectively; 132 were resistant to at least one of the four anti-tuberculosis drugs and 51 were multi-drug resistant (MDR). Beijing genotype and retreated patients showed a significantly increased risk for developing MDR tuberculosis. Compared with the phenotypic data, the sensitivity and specificity for WGS to predict resistance were 96.7% and 98.6% for RMP, 75.5% and 97.1% for INH, 68.6% and 99.6% for STR, 93.1% and 93.7% for EMB, respectively. The most common mutations conferring RMP, INH, STR and EMB resistance were Ser450Leu (51.7%) in , Ser315Thr (44.3%) in , Lys43Arg (35.7%) in and Met306Val (24.1%) in .

Conclusion: This study provides the first information on the prevalence and molecular characters of drug resistant in the southern region of Xinjiang, China, which will be helpful for choosing early detection methods for drug resistance (ig, molecular methods) and subsequently initiation of proper therapy of tuberculosis in this area.
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http://dx.doi.org/10.2147/IDR.S320024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402983PMC
August 2021

Progress and challenges of network meta-analysis.

J Evid Based Med 2021 Sep 30;14(3):218-231. Epub 2021 Aug 30.

Public Health and Health Services Research, Norwich Medical School, University of East Anglia, Norwich, UK.

In the past years, network meta-analysis (NMA) has been widely used among clinicians, guideline makers, and health technology assessment agencies and has played an important role in clinical decision-making and guideline development. To inform further development of NMAs, we conducted a bibliometric analysis to assess the current status of published NMA methodological studies, summarized the methodological progress of seven types of NMAs, and discussed the current challenges of NMAs.
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http://dx.doi.org/10.1111/jebm.12443DOI Listing
September 2021

Reduced LINC00467 elevates microRNA-125a-3p to suppress cisplatin resistance in non-small cell lung cancer through inhibiting sirtuin 6 and inactivating the ERK1/2 signaling pathway.

Cell Biol Toxicol 2021 Aug 30. Epub 2021 Aug 30.

Department of Radiotherapy, Affiliated Hospital of Guilin Medical University, No.15 Lequn Road, Guilin, 541001, Guangxi, China.

Objective: The non-coding RNAs have been identified as potential biomarkers in cancers. This study aimed to investigate the effect of long intergenic non-protein coding RNA 467 (LINC00467)/microRNA (miR-125a-3p)/sirtuin 6 (SIRT6) axis on non-small cell lung cancer (NSCLC).

Methods: Expression of LINC00467, miR-125a-3p, SIRT6, and ERK1/2 signaling pathway-related factor in NSCLC was assessed. A549 and H1299 cells were induced by cisplatin (DDP) to establish DDP-resistant cell lines, and the drug resistance was detected. DDP-resistant cells were transfected with relative oligonucleotides and/or plasmids to observe the effects of LINC00467 and miR-125a-3p on NSCLC cells. The interaction among LINC00467, miR-125a-3p, and SIRT6 was detected using dual luciferase reporter gene assay and RNA pull-down assay. A tumor xenograft model was established, and Ki67 expression was detected in tumors.

Results: LINC00467 and SIRT6 were upregulated, miR-125a-3p was suppressed, and the ERK1/2 pathway was activated in NSCLC. LINC00467 or SIRT6 inhibition or miR-125a-3p elevation restricted malignancy and DDP resistance of NSCLC cells. Reduced miR-125a-3p reversed the effect of inhibited LINC00467 on NSCLC cells. LINC00467 competitively bound to miR-125a-3p, and miR-125a-3p targeted SIRT6. Inhibiting LINC00467 and SIRT6 or upregulating miR-125a-3p repressed tumor formation in vivo.

Conclusion: LINC00467 silencing upregulates miR-125a-3p to decrease DDP resistance in NSCLC cells via inhibiting SIRT6 and inactivating the ERK1/2 signaling pathway.
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http://dx.doi.org/10.1007/s10565-021-09637-6DOI Listing
August 2021

Research on Quality Characterization Method of Micro-Injection Products Based on Cavity Pressure.

Polymers (Basel) 2021 Aug 17;13(16). Epub 2021 Aug 17.

National Local Joint Engineering Laboratory of Intelligent Manufacturing Oriented Automobile Die & Mold, Tianjin University of Technology and Education, Tianjin 300222, China.

The cavity pressure in the injection molding process is closely related to the quality of the molded products, and is used for process monitoring and control, to upgrade the quality of the molded products. The experimental platform was built to carry out the cavity pressure experiment with a micro spline injection mold in the paper. The process parameters were changed, such as V/P switchover, mold temperature, melt temperature, packing pressure, and injection rate, in order to analyze the influence of the process parameters on the product weight. The peak cavity pressure and area under the pressure curve were the two attributes utilized in investigating the correlation between cavity pressure and part weight. The experimental results show that the later switchover allowed the injection to proceed longer and produce a heavier tensile specimen. By comparing different cavity pressure curves, the general shapes of the curves were able to indicate different types of shortage produced. When the V/P switchover position is 10 mm, the coefficient of determination (R value) of part weight, for the peak cavity pressure and area under the curve, were 0.7706 and 0.8565, respectively. This showed that the area under the curve appeared to be a better process and quality indicator than the peak cavity pressure.
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http://dx.doi.org/10.3390/polym13162755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398340PMC
August 2021

Microalgae preparation and lipid extraction by subcritical dimethyl ether.

MethodsX 2021 20;8:101353. Epub 2021 Apr 20.

Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Nishikyo-Ku, Kyoto, Japan.

Biodiesel produced from microalgae is a potential alternative due to the high growth rate of microalgae, the possibility of using nonarable land, and high lipid accumulation rate. Microalgae cultivation, cell harvesting and disruption are the important steps before lipid extraction for the biodiesel. In the co-submission article, the details of the whole process cannot be clearly explained. In this regard, we present the details of methods on parameter of photo-bioreactor for cultivating microalgae, flocculation tests to determine optimal flocculant dosage in harvesting, parameter of Dimethyl ether (DME) subcritical extraction device and full-factorial design for investigating the influence of extraction time, initial water content and DME dosage on the extraction performance. It will allow researchers to reproduce these experiments. • The method shows a cell disruption assisted lipid extraction by subcritical dimethyl ether. • Model is built from full-factorial design to investigate multi-factor influence. • Differential scanning calorimetry can be applicable to measure free water content.
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http://dx.doi.org/10.1016/j.mex.2021.101353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374345PMC
April 2021

PEG-Polymer Encapsulated Aggregation-Induced Emission Nanoparticles for Tumor Theranostics.

Adv Healthc Mater 2021 Aug 19:e2101036. Epub 2021 Aug 19.

Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430032, China.

In the field of tumor imaging and therapy, the aggregation-caused quenching (ACQ) effect of fluorescent dyes at high concentration is a great challenge. In this regard, the aggregation-induced emission luminogens (AIEgens) show great potential, since AIEgens effectively overcome the ACQ effect and have better fluorescence quantum yield, photobleaching resistance, and photosensitivity. Polyethylene glycol (PEG)-polymer is the most commonly used carrier to prepare nanoparticles (NPs). The advantage of PEGylation is that it can greatly prolong the metabolic half-life and reduce immunogenicity and toxicity. Considering that the hydrophobicity of most AIEgens hinders their application in organisms, the use of PEG-polymer encapsulation is an effective strategy to overcome this obstacle. Importantly, bioactive functional groups can be modified on PEG-polymers to enhance the biological effect of NPs. The combination of powerful AIEgens and PEG-polymers provides a new strategy for tumor imaging and therapy, which is promising for clinical application.
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http://dx.doi.org/10.1002/adhm.202101036DOI Listing
August 2021

Current Trends and Research Topics Regarding Intestinal Organoids: An Overview Based on Bibliometrics.

Front Cell Dev Biol 2021 3;9:609452. Epub 2021 Aug 3.

Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing, China.

Currently, research on intestinal diseases is mainly based on animal models and cell lines in monolayers. However, these models have drawbacks that limit scientific advances in this field. Three-dimensional (3D) culture systems named organoids are emerging as a reliable research tool for recapitulating the human intestinal epithelium and represent a unique platform for patient-specific drug testing. Intestinal organoids (IOs) are crypt-villus structures that can be derived from adult intestinal stem cells (ISCs), embryonic stem cells (ESCs), or induced pluripotent stem cells (iPSCs) and have the potential to serve as a platform for individualized medicine and research. However, this emerging field has not been bibliometric summarized to date. Here, we performed a bibliometric analysis of the Web of Science Core Collection (WoSCC) database to evaluate 5,379 publications concerning the use of organoids; the studies were divided into four clusters associated with the current situation and future directions for the application of IOs. Based on the results of our bibliometric analysis of IO applications, we systematically summarized the latest advances and analyzed the limitations and prospects.
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http://dx.doi.org/10.3389/fcell.2021.609452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369504PMC
August 2021

Clinical features of gastric duplications: evidence from primary case reports and published data.

Orphanet J Rare Dis 2021 08 19;16(1):368. Epub 2021 Aug 19.

Department of Gastroenterological Surgery, Laboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People's Hospital, No.11 Xizhimen South Street, Xicheng District, Beijing, 100044, China.

Background: Alimentary tract duplications are rare congenital lesions, and only 2-8% of them are located in the stomach. Gastric duplications (GD) can lead to severe adverse events. Thus, surgical resection is required once the disease is diagnosed. The main purpose of this study is to describe the clinical features of gastric duplications and to provide evidence for the diagnosis and treatment.

Methods: A retrospective review of eight gastric duplications at two medical centers Peking University People's Hospital (PKUPH) and Shandong Provincial Hospital from 2010 to 2020 was conducted. Furthermore, the literature search was also conducted by retrieving data from PubMed, EMBASE and Cochrane Library databases from the date of the database inception to January 15, 2021.

Results: Eight patients who were diagnosed as gastric duplications and 311 published records were included in this study. In all, 319 patients were identified: Vomiting and abdominal pain were the most frequent clinical presentations among juveniles and adults respectively. There was no difference in gender distribution (F: 53.16% vs M: 46.84%), and the cystic gastric duplications were the most common type of the gastric duplications (87.04%). More than half (53.30%) of included cases were located in the greater curvature of stomach.

Conclusions: Gastric duplications could present with a wide spectrum of symptomatology, which might be misdiagnosed easily as other diseases. For cystic gastric duplications, the optimal treatment was a complete surgical removal. But conservative treatment might be an alternative strategy for tubular gastric duplications.
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http://dx.doi.org/10.1186/s13023-021-01992-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377950PMC
August 2021

Discovery of Novel Apigenin-Piperazine Hybrids as Potent and Selective Poly (ADP-Ribose) Polymerase-1 (PARP-1) Inhibitors for the Treatment of Cancer.

J Med Chem 2021 Aug 18;64(16):12089-12108. Epub 2021 Aug 18.

State Key Laboratory of Natural Medicines, Department of TCMs Pharmaceuticals, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, People's Republic of China.

Poly (ADP-ribose) polymerase-1 (PARP-1) is a potential target for the discovery of chemosensitizers and anticancer drugs. Amentoflavone () is reported to be a selective PARP-1 inhibitor. Here, structural modifications and trimming of have led to a series of derivatives () and apigenin-piperazine/piperidine hybrids (, , , and ), respectively. Among these compounds, exhibited a potent PARP-1 inhibitory effect (IC = 14.7 nM) and possessed high selectivity to PARP-1 over PARP-2 (61.2-fold). Molecular dynamics simulation and the cellular thermal shift assay revealed that directly bound to the PARP-1 structure. In and studies, showed a potent chemotherapy sensitizing effect against A549 cells and a selective cytotoxic effect toward SK-OV-3 cells through PARP-1 inhibition. also displayed good ADME characteristics, pharmacokinetic parameters, and a desirable safety margin. These findings demonstrated that may serve as a lead compound for chemosensitizers and the (BRCA-1)-deficient cancer therapy.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00735DOI Listing
August 2021

Loci and natural alleles for cadmium-mediated growth responses revealed by a genome wide association study and transcriptome analysis in rice.

BMC Plant Biol 2021 Aug 13;21(1):374. Epub 2021 Aug 13.

Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.

Background: Cadmium (Cd) is a toxic heavy metal that is harmful to the environment and human health. Cd pollution threatens the cultivation of rice (Oryza sativa L.) in many countries. Improving rice performance under Cd stress could potentially improve rice productivity.

Results: In this study, 9 growth traits of 188 different cultivated rice accessions under normal and Cd stress conditions were found to be highly variable during the seedling stage. Based on ~3.3 million single nucleotide polymorphisms (SNPs), 119 Cd-mediated growth response (CGR) quantitative trait loci (QTL) were identified by a genome-wide association study (GWAS), 55 of which have been validated by previously reported QTL and 64 were new CGR loci. Combined with the data from the GWAS, transcriptome analysis, gene annotations from the gene ontology (GO) Slim database, and annotations and functions of homologous genes, 148 CGR candidate genes were obtained. Additionally, several reported genes have been found to play certain roles in CGRs. Seven Cd-related cloned genes were found among the CGR genes. Natural elite haplotypes/alleles in these genes that increased Cd tolerance were identified by a haplotype analysis of a diverse mini core collection. More importantly, this study was the first to uncover the natural variations of 5 GST genes that play important roles in CGRs.

Conclusion: The exploration of Cd-resistant rice germplasm resources and the identification of elite natural variations related to Cd-resistance will help improve the tolerance of current major rice varieties to Cd, as well as provide raw materials and new genes for breeding Cd-resistant varieties.
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http://dx.doi.org/10.1186/s12870-021-03145-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8362254PMC
August 2021

Altered thalamo-cortical functional connectivity in patients with vestibular migraine: a resting-state fMRI study.

Neuroradiology 2021 Aug 10. Epub 2021 Aug 10.

Department of Neurology, Second Affiliated Hospital of Xuzhou Medical University, No. 32, Meijian Road, Xuzhou, 221006, Jiangsu, China.

Purpose: To explore the functional connectivity (FC) between the bilateral thalamus and the other brain regions in patients with vestibular migraine (VM).

Methods: Resting-state fMRI and 3D-T1 data were collected from 37 patients with VM during the interictal period and 44 age-, gender-, and years of education-matched healthy controls (HC). The FC of the bilateral thalamus was analyzed using a standard seed-based whole-brain correlation method. Furthermore, the correlations between thalamus FC and clinical characteristics of patients were investigated using Pearson's partial correlation.

Results: Compared with HC, VM patients showed decreased FC between the left thalamus and the left anterior cingulate cortex (ACC), bilateral insular and right supplementary motor cortex. We also observed decreased FC between the right thalamus and the left insular and ACC in VM patients. Furthermore, patients with VM also exhibited increased FC between the left thalamus and the right precuneus and middle frontal gyrus, between the right thalamus and superior parietal lobule. FC between the right thalamus and the left insular was negatively correlated with disease duration (p = 0.019, r =  - 0.399), FC between the left thalamus and the left ACC was negatively correlated with HIT-6 score (p = 0.004, r =  - 0.484).

Conclusion: VM patients showed altered FC between thalamus and brain regions involved in pain, vestibular and visual processing, which are associated with specific clinical features. Specifically, VM patients showed reduced thalamo-pain and thallamo-vestibular pathways, while exhibited enhanced thalamo-visual pathway, which provided first insight into the underlying functional brain connectivity in VM patients.
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http://dx.doi.org/10.1007/s00234-021-02777-wDOI Listing
August 2021

Precise Targeting Therapy of Orthotopic Gastric Carcinoma by siRNA and Chemotherapeutic Drug Codelivered in pH-Sensitive Nano Platform.

Adv Healthc Mater 2021 Oct 7;10(20):e2100966. Epub 2021 Aug 7.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200032, China.

Gastric cancer is one of the most common malignant tumors, which remains as an obstacle to human health. Nowadays, targeted nanoparticles to gastric tumor tissues, provide new strategy for improved therapy but still remain challenging. The major hurdle of targeted therapeutic nanoparticles comes from the limited enrichment and poor selectivity of therapeutic agents in in situ tumor. Herein, a pH-sensitive targeted nano platform coloaded As O and human epidermal growth factor receptor-2 (HER2)-siRNA (AH RNPs) is developed to achieve targeting therapy in orthotopic gastric carcinoma. AH RNPs can effectively prevent the degradation of siRNA and overcome the poor solubility of As O . In vitro studies show that AH RNPs could achieve synergistic inhibition of growth and metastasis on SGC7901 cells. Surprisingly, AH RNPs not only target gastric subcutaneous tumor, but also target in situ tumor, and express loaded genes in in situ tumor. Moreover, AH RNPs show excellent antitumor effect in orthotopic gastric tumor model and the anticancer mechanism is related about inhibiting the activation of ERK signal and downregulating the expression of cxc chemokine receptor 4 (CXCR4), HER2, MMP2, and MMP9 protein. This study provides a multi-functional vector for precise targeting therapy of gastric cancer, which may serve as a potential clinical application for future gastric cancer.
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http://dx.doi.org/10.1002/adhm.202100966DOI Listing
October 2021
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