Publications by authors named "Quan Liu"

722 Publications

Ground glass opacity featured lung adenocarcinoma in teenagers.

J Cancer Res Clin Oncol 2021 Apr 7. Epub 2021 Apr 7.

Department of Thoracic Surgery and State Key Laboratory of Genetic Engineering, Fudan University Shanghai Cancer Center, 270 Dong-An Road, Shanghai, 200032, China.

Introduction: Ground glass opacity (GGO) nodules were found incidentally by computed tomography (CT) scan in some teenagers, which turned out to be lung cancer. The purpose of this study is to summarize the characteristics of teenage patients with GGO featured lung adenocarcinoma.

Methods: Patients aging from 13 to 20 who were incidentally diagnosed with lung cancer were reviewed between February 2015 to December 2020. The clinical, radiological and pathological characteristics were analyzed.

Results: Totally 12 patients were included. All of them were diagnosed as GGO featured lung cancer through CT scan, with no presenting symptom. The median surveillance before surgery was 5.5 months, and none of these GGO lesions enlarged or altered in the property during the surveillance. The mean tumor diameter was 0.93 ± 0.25 cm. Ten patients underwent wedge resection by video-assisted thoracoscopic surgery (VATS), 9 of which were minimally invasive adenocarcinoma (MIA) and 1 of which were invasive adenocarcinoma (IAC) in the pathological analysis. One patient underwent VATS left-upper sublobectomy, pathologically diagnosed as MIA and 1 patient underwent VATS left-upper lobectomy with systematic mediastinal lymphadenectomy, pathologically diagnosed as IAC. The median postoperative hospital stay was 3 days. All patients survived without recurrence during a median follow-up of 12.5 months.

Conclusions: GGO nodules could be a sign of early-stage teenage lung adenocarcinoma. We proposed a screening strategy with long intervals based on a baseline CT scan for the teenage population, and a treatment strategy for diagnosed teenage patients.
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http://dx.doi.org/10.1007/s00432-021-03611-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026089PMC
April 2021

Online detection of class-imbalanced error-related potentials evoked by motor imagery.

J Neural Eng 2021 Apr 6. Epub 2021 Apr 6.

, School of Information Engineering, Wuhan University of Technology, 122 Luoshi Road, Hongshan District, Wuhan, Hubei, 430070, CHINA.

Objective: Error-related potentials (ErrPs) are spontaneous electroencephalogram (EEG) signals related to the awareness of erroneous responses within brain domain. Many studies on ErrPs detection are mostly conducted under offline conditions with poor classification accuracy and the error rates of ErrPs are preset in advance. In order to solve these problems, a novel method based on adaptive autoregressive (AAR) model and common spatial pattern (CSP) is proposed for ErrPs feature extraction. In addition, an adaptive threshold classification method based spectral regression discriminant analysis is suggested for class-unbalanced ErrPs data to reduce the false positives and false negatives.

Approach: As for ErrPs feature extraction, the AAR coefficients in the temporal domain and CSP in the spatial domain are fused. Given that the performance of different subjects' MI tasks is different but stable, and the samples of ErrPs are class-imbalanced, an adaptive threshold based spectral regression discriminant analysis (SRDA) is suggested for classification. Two datasets are used in this paper.

Main Results: The experimental results on the CNBI dataset show that the average accuracy and false positive rate for ErrPs detection are 94.1% and 8.1%, which outperforms methods using features extracted from a single domain. What's more, although the ErrPs induction rate is affected by the performance of subjects' MI-BCI tasks, experimental results on data recorded in the self-designed system prove that the ErrPs classification algorithm based on an adaptive threshold is robust under different ErrPs data distributions. Compared with two other methods, the proposed algorithm has advantages in all three measures which are accuracy, F1-score and false positive rate. Finally, ErrPs detection results were used to prevent wrong actions in a MI-BCI experiment, and it leads to a reduction of the hybrid BCI error rate from 48.9% to 24.3% in online tests.

Significance: Both the AAR-CSP fused feature extraction and the adaptive threshold based SRDA classification methods suggested in our work are efficient in improving the ErrPs detection accuracy and reducing the false positives. In addition, by introducing ErrPs to multi-class MI-BCIs, the MI decoding results can be corrected after ErrPs is detected to avoid executing wrong instructions, thereby improving the BCI accuracy and lays the foundation for using MI-BCIs in practical applications.
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http://dx.doi.org/10.1088/1741-2552/abf522DOI Listing
April 2021

Monocytes promote pyroptosis of endothelial cells during lung ischemia-reperfusion via IL-1R/NF-κB/NLRP3 signaling.

Life Sci 2021 Mar 27;276:119402. Epub 2021 Mar 27.

Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

In our previous study, we observed that donor pulmonary intravascular nonclassical monocytes play a major role in early PGF, but the specific mechanism remained unclear. In this study, we investigated the mechanistic role of monocytes in inducing pyroptosis of human pulmonary microvascular endothelial cells (HPMECs) during IRI. A murine hilar ligation model of IRI was utilized whereby left lungs underwent 1 h of ischemia and 23 h of reperfusion. Monocyte depletion by intraperitoneal clodronate-liposome treatment on pulmonary edema and pyroptosis activation were determined. In vitro experiments, we performed the co-culture experiments under hypoxia-reoxygenation (H/R) conditions to mimic the IRI environment. We monitored the expression of NLRP3, caspase-1 and IL-1β in co-cultures of monocytes (U937 cells) and HPMECs under H/R conditions. NLRP3, IL-1β and IL-1R siRNA knockdown, caspase-1 and NF-κB pathway inhibitors were employed to elucidate the mechanism modulating HPMEC pyroptosis during H/R. Treatment of mice with clodronate-liposome attenuated IR-induced pulmonary edema, cytokine production and pyroptosis activation. In vitro, NLRP3 knockdown in monocytes reduced caspase-1 and IL-1β secretion in co-cultures of monocytes and HPMECs. Reduced HPMEC pyroptosis was also observed either containing HPMECs with genetically engineered IL-1R knockdown or in co-culture treated with a Triplotide inhibitor that disrupts NF-κB signaling. Monocytes play a vital role in the development of transplant-associated ischemia-reperfusion injury. A potential role is that monocytes secrete IL-1β to induce HPMEC pyroptosis via the IL-1R/NF-κB/NLRP3 pathway.
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http://dx.doi.org/10.1016/j.lfs.2021.119402DOI Listing
March 2021

Ambient marine shipping emissions determined by vessel operation mode along the East China Sea.

Sci Total Environ 2021 May 20;769:144713. Epub 2021 Jan 20.

State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 100084, PR China.

Marine shipping emissions exert important air quality and climate impacts. This study characterized the ambient pollutants predominant by emissions from a variety of marine vessel types near the mid-latitude East China Sea. Two discernible primary shipping emissions were identified by factorization analysis on detailed mass spectra of organic aerosol (OA), as emissions in maneuvering and cruise, highly linked with NO (and less oxidized OA, black carbon, BC) or CO (and more oxidized OA), respectively. Using radio-recorded quantities and activities of 3566 vessels mixed with slow and high-speed diesel engines, we found emission of NO or BC per vessel was positively correlated with vessel speed, while CO emission peaked at moderate speed. The approach here based on vessel operation mode directly linked the vessel activities to ambient concentrations of pollutants from marine shipping emission, and may synthesize the complex vessel types in shipping emission inventory.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144713DOI Listing
May 2021

Ventricular fibrillation storm after revascularization of chronic total occlusion of the left anterior descending artery: is this reperfusion arrhythmia?

J Int Med Res 2021 Mar;49(3):300060521997618

Department of Cardiology, the First Hospital of Jilin University, Changchun, Jilin Province, China.

Electrical storm is a life-threatening emergency condition defined as three or more episodes of ventricular tachycardia or ventricular fibrillation (VF) within 24 hours requiring anti-tachycardia therapy, electrical cardioversion, or defibrillation. However, studies of the incidence of electrical storm after chronic total occlusion-percutaneous coronary intervention (CTO-PCI) are limited, and post-procedural VF after revascularization of CTO has not been described. The purpose of this article was to present a case of post-operative VF electrical storm after revascularization of CTO of the left anterior descending (LAD) artery to determine whether the electrical storm was caused by reperfusion arrhythmia or compromise of either branch vessels or the collateral circulation during intervention.
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http://dx.doi.org/10.1177/0300060521997618DOI Listing
March 2021

A CT-Based Radiomics Approach to Predict Nivolumab Response in Advanced Non-Small-Cell Lung Cancer.

Front Oncol 2021 24;11:544339. Epub 2021 Feb 24.

Department of Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.

Purpose: This study aims to develop a CT-based radiomics model to predict clinical outcomes of advanced non-small-cell lung cancer (NSCLC) patients treated with nivolumab.

Methods: Forty-six stage IIIB/IV NSCLC patients without EGFR mutation or ALK rearrangement who received nivolumab were enrolled. After segmenting primary tumors depicting on the pre-anti-PD1 treatment CT images, 1,106 radiomics features were computed and extracted to decode the imaging phenotypes of these tumors. A L1-based feature selection method was applied to remove the redundant features and build an optimal feature pool. To predict the risk of progression-free survival (PFS) and overall survival (OS), the selected image features were used to train and test three machine-learning classifiers namely, support vector machine classifier, logistic regression classifier, and Gaussian Naïve Bayes classifier. Finally, the overall patients were stratified into high and low risk subgroups by using prediction scores obtained from three classifiers, and Kaplan-Meier survival analysis was conduct to evaluate the prognostic values of these patients.

Results: To predict the risk of PFS and OS, the average area under a receiver operating characteristic curve (AUC) value of three classifiers were 0.73 ± 0.07 and 0.61 ± 0.08, respectively; the corresponding average Harrell's concordance indexes for three classifiers were 0.92 and 0.79. The average hazard ratios (HR) of three models for predicting PFS and OS were 6.22 and 3.54, which suggested the significant difference of the two subgroup's PFS and OS (p<0.05).

Conclusion: The pre-treatment CT-based radiomics model provided a promising way to predict clinical outcomes for advanced NSCLC patients treated with nivolumab.
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http://dx.doi.org/10.3389/fonc.2021.544339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943844PMC
February 2021

Equivalent efficacy study of QL1101 and bevacizumab on untreated advanced non-squamous non-small cell lung cancer patients: a phase 3 randomized, double-blind clinical trial.

Cancer Biol Med 2021 Mar 12. Epub 2021 Mar 12.

Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China.

Objective: This phase 3 study aimed to test equivalence in efficacy and safety for QL1101, a bevacizumab analogue in Chinese patients with untreated locally advanced non-squamous non-small cell lung cancer (NSCLC).

Methods: Eligible patients were randomly assigned 1:1 to receive carboplatin and paclitaxel in combination with either QL1101 or bevacizumab, 15 mg/kg every 3-week for 6 cycles. This was followed by maintenance treatment with single agent QL1101 every 3-week. The primary end-point was objective response rate (ORR), with secondary end-points being progression-free survival (PFS), overall survival (OS), disease control rate (DCR), and adverse events (AEs).

Results: Of 675 patients, 535 eligible patients were randomized to the QL1101 group ( = 269) and bevacizumab group ( = 266). ORRs were 52.8% and 56.8%, respectively, for the QL1101 and bevacizumab groups, with an ORR hazard ratio 0.93 (95% confidence interval: 0.8-0131.1). The PFS, OS, DCR, and AEs were comparable between the 2 groups, which remained the same after stratification according to epidermal growth factor receptor mutation or smoking history.

Conclusions: QL1101 showed similar efficacy and safety profiles as compared to bevacizumab among Chinese patients with untreated locally advanced non-squamous NSCLC.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0212DOI Listing
March 2021

Tree frog-inspired nanopillar arrays for enhancement of adhesion and friction.

Biointerphases 2021 Mar 4;16(2):021001. Epub 2021 Mar 4.

School of Power and Mechanical Engineering and The Institute of Technological Science Wuhan University, South Donghu Road 8, Wuhan, Hubei 430072, China.

Bioinspired structure adhesives have received increasing interest for many applications, such as climbing robots and medical devices. Inspired by the closely packed keratin nanopillars on the toe pads of tree frogs, tightly arranged polycaprolactone nanorod arrays are prepared by mold process and chemical modification. Nanorod arrays show enhanced adhesion and friction on both smooth and rough surfaces compared to the arrays with hexagonal micropillars. The bonding of nanorods results in a larger stiffness of the nanorod surface, contributing mainly to friction rather than adhesion. The results suggest the function of closely packed keratin nanopillars on the toe pad of tree frogs and offer a guiding principle for the designing of new structured adhesives with strong attaching abilities.
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http://dx.doi.org/10.1116/6.0000747DOI Listing
March 2021

Hypoxia associated multi-omics molecular landscape of tumor tissue in patients with hepatocellular carcinoma.

Aging (Albany NY) 2021 Mar 10;13(5):6525-6553. Epub 2021 Mar 10.

Department of Hepatobiliary and Pancreas Surgery, The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Shenzhen 518020, Guangdong, China.

The present study was designed to update the knowledge about hypoxia-related multi-omic molecular landscape in hepatocellular carcinoma (HCC) tissues. Large-size HCC datasets from multiple centers were collected. The hypoxia exposure of tumor tissue from patients in 10 HCC cohorts was estimated using a novel HCC-specific hypoxia score system constructed in our previous study. A comprehensive bioinformatical analysis was conducted to compare hypoxia-associated multi-omic molecular features in patients with a high hypoxia score to a low hypoxia score. We found that patients with different exposure to hypoxia differed significantly in transcriptomic, genomic, epigenomic, and proteomic alterations, including differences in mRNA, microRNA (miR), and long non-coding RNA (lncRNA) expression, differences in copy number alterations (CNAs), differences in DNA methylation levels, differences in RNA alternative splicing events, and differences in protein levels. HCC survival- associated molecular events were identified. The potential correlation between molecular features related to hypoxia has also been explored, and various networks have been constructed. We revealed a particularly comprehensive hypoxia-related molecular landscape in tumor tissues that provided novel evidence and perspectives to explain the role of hypoxia in HCC. Clinically, the data obtained from the present study may enable the development of individualized treatment or management strategies for HCC patients with different levels of hypoxia exposure.
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http://dx.doi.org/10.18632/aging.202723DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993683PMC
March 2021

Diphenyl diselenide ameliorates diabetic nephropathy in streptozotocin-induced diabetic rats via suppressing oxidative stress and inflammation.

Chem Biol Interact 2021 Apr 24;338:109427. Epub 2021 Feb 24.

Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518057, China. Electronic address:

Oxidative stress and inflammation are implicated in the occurrence and progression of diabetic nephropathy (DN). Diphenyl diselenide (DPDS) is a stable and simple diaryl diselenide with anti-hyperglycemic, anti-inflammatory, and antioxidant activities. However, the effects of DPDS on DN are still unclear to date. Herein, we aimed to explore whether DPDS could improve renal dysfunction in streptozotocin (STZ)-induced diabetic rats and its underlying mechanisms. STZ-induced DN rats were administered with DPDS (5 or 15 mg/kg) or metformin (200 mg/kg) once daily by intragastric gavage for 12 weeks. DPDS supplementation significantly improved hyperglycemia, glucose intolerance, dyslipidemia, and the renal pathological abnormalities, concurrent with significantly reduced serum levels of creatinine, urea nitrogen, urine volume, and urinary levels of micro-albumin, β2-microglobulin and N-acetyl-glucosaminidase activities. Moreover, DPDS effectively promoted the activities of antioxidant enzymes, and reduced the levels of MDA and pro-inflammatory factors in serum and the kidney. Furthermore, DPDS supplementation activated the renal Nrf2/Keap1 signaling pathway, but attenuated the high phosphorylation levels of NFκB, JNK, p38 and ERK1/2. Altogether, the current study indicated for the first time that DPDS ameliorated STZ-induced renal dysfunction in rats, and its mechanism of action may be attributable to suppressing oxidative stress via activating the renal Nrf2/Keap1 signaling pathway and mitigating inflammation by suppressing the renal NFκB/MAPK signaling pathways, suggesting a potential therapeutic approach for DN.
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http://dx.doi.org/10.1016/j.cbi.2021.109427DOI Listing
April 2021

Immediate Implant Placement with Buccal Bone Augmentation in the Anterior Maxilla with Thin Buccal Plate: A One-Year Follow-Up Case Series.

J Prosthodont 2021 Feb 27. Epub 2021 Feb 27.

Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.

Purpose: To evaluate the buccal bone thickness of immediate implant placement with buccal bone augmentation in patients with a thin buccal plate in the esthetic zone.

Materials And Methods: Eighteen consecutive patients requiring a single tooth replacement in the anterior maxillary zone with a thin plate (<1 mm) were included and received immediate implant placement with narrow-diameter implants. Patients received buccal bone augmentation (both internal and external socket bone grafting) with deproteinized bovine bone mineral (DBBM) and an absorbable membrane. The final restoration was delivered after 8 months. Cone-beam CT scans were performed before surgery (CBCT0), immediately after surgery (CBCT1), at final restoration delivery (CBCT2), and at 1-year follow-up after the final restoration (CBCT3) to evaluate the buccal bone thickness and ridge width. A repeated measures ANOVA and Bonferroni correction for multiple comparisons were applied for statistical analysis of changes within different time points (α = 0.05).

Results: Fifteen of the 18 enrolled patients were available for analysis at the 1-year follow-up after final restoration. The mean buccal bone thickness at 2 mm apical to the implant-abutment junction (IAJ-2) were 3.59 mm (range: 3.04-4.58 mm), 2.79 mm (range: 2.25-3.78 mm), and 2.52 mm (range: 1.72-3.36 mm), respectively, at CBCT1, CBCT2, and CBCT3. A statistical significance was observed for buccal bone thickness change between CBCT1 and CBCT2 at IAJ-2 (F = 17.948, p = 0.001). The net gains of the ridge width from CBCT0 to CBCT1, CBCT1 to CBCT2, and CBCT2 to CBCT3 were 1.08 mm, -0.94 mm and -0.04 mm at 4 mm apical to the cementum-enamel junction, respectively. No statistical significance was observed for the change in ridge width from CBCT0 to CBCT3 (F = 10.518, p = 1.000).

Conclusions: Simultaneous buccal bone augmentation may maintain a predictable buccal bone thickness for immediate implant placement in the maxillary anterior sites with a thin buccal plate (<1 mm) at 1-year follow-up after final restoration.
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http://dx.doi.org/10.1111/jopr.13350DOI Listing
February 2021

Inhibiting microRNA-155 attenuates atrial fibrillation by targeting CACNA1C.

J Mol Cell Cardiol 2021 Feb 24;155:58-65. Epub 2021 Feb 24.

Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; Medical School of University of Chinese Academy of Sciences, Beijing, China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China. Electronic address:

Background: Reduction in L-type Ca current (I) density is a hallmark of the electrical remodeling in atrial fibrillation (AF). The expression of miR-155, whose predicted target gene is the α1c subunit of the calcium channel (CACNA1C), was upregulated in atrial cardiomyocytes (aCMs) from patients with paroxysmal AF.The study is to determine miR-155 could target the gene expression of I and contribute to electrical remodeling in AF.

Methods: The expression of miR-155 and CACNA1C was assessed in aCMs from patients with paroxysmal AF and healthy control. I properties were observed after miR-155 transfection in human induced pluripotent stem cell derived atrial cardiomyocytes (hiPSC-aCMs). Furthermore, an miR-155 transgene (Tg) and knock-out (KO) mouse model was generated to determine whether miR-155 was involved in I-related electrical remodeling in AF by targeting CACNA1C.

Results: The expression level of miR-155 was increased, while the expression level of CACNA1C reduced in the aCMs of patients with AF. miR-155 transfection in hiPSC-aCMs produced changes in I properties qualitatively similar to those produced by AF. miR-155/Tg mice developed a shortened action potential duration and increased vulnerability to AF, which was associated with decreased I and attenuated by an miR-155 inhibitor. Finally, the genetic inhibition of miR-155 prevented AF induction in miR-155/KO mice with no changes in I properties.

Conclusions: The increased miR-155 expression in aCMs was sufficient for the reduction in the density of I and the underlying electronic remodeling. The inhibition of miR-155 prevented I-related electric remodeling in AF and might constitute a novel anti-AF approach targeting electrical remodeling.
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http://dx.doi.org/10.1016/j.yjmcc.2021.02.008DOI Listing
February 2021

A novel hypoxia gene signature indicates prognosis and immune microenvironments characters in patients with hepatocellular carcinoma.

J Cell Mol Med 2021 Feb 22. Epub 2021 Feb 22.

Department of Hepatobiliary and Pancreas Surgery, The Second Clinical Medical College, Jinan University (Shenzhen People's Hospital), Shenzhen, Guangdong, China.

Due to the lack of a suitable gene signature, it is difficult to assess the hypoxic exposure of HCC tissues. The clinical value of assessing hypoxia in HCC is short of tissue-level evidence. We tried to establish a robust and HCC-suitable hypoxia signature using microarray analysis and a robust rank aggregation algorithm. Based on the hypoxia signature, we obtained a hypoxia-associated HCC subtypes system using unsupervised hierarchical clustering and a hypoxia score system was provided using gene set variation analysis. A novel signature containing 21 stable hypoxia-related genes was constructed to effectively indicate the exposure of hypoxia in HCC tissues. The signature was validated by qRT-PCR and compared with other published hypoxia signatures in multiple large-size HCC cohorts. The subtype of HCC derived from this signature had different prognosis and other clinical characteristics. The hypoxia score obtained from the signature could be used to indicate clinical characteristics and predict prognoses of HCC patients. Moreover, we reveal a landscape of immune microenvironments in patients with different hypoxia score. In conclusion, we identified a novel HCC-suitable 21-gene hypoxia signature that could be used to estimate the hypoxia exposure in HCC tissues and indicated prognosis and a series of important clinical features in HCCs. It may enable the development of personalized counselling or treatment strategies for HCC patients with different levels of hypoxia exposure.
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http://dx.doi.org/10.1111/jcmm.16249DOI Listing
February 2021

Para-aortic lymph node metastasis in lower Thoracic Esophageal Squamous Cell Carcinoma after Radical Esophagectomy: a CT-based atlas and its clinical implications for Adjuvant Radiotherapy.

J Cancer 2021 18;12(6):1734-1741. Epub 2021 Jan 18.

Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, China.

Our previous work showed that para-aortic lymph node (PALN) metastasis was the major failure pattern in lower thoracic esophageal squamous cell carcinoma (LTESCC) patients who presented abdominal LN failure after curative surgery. We thereby aim to generate a computerized tomography (CT)-based documentation of PALNs and to propose a clinical target volume (CTV) for this region. Sixty-five patients were enrolled. The epicentre of each PALN was drawn onto an axial CT image of a standard patient with reference to the surrounding anatomical landmarks. A CTV for PALN was generated based on the final result of node distribution, and was evaluated for dosimetric performance in three simulated patients. All the studied 248 LNs were below the level of 1.0 cm above the celiac artery (CA), and 94.76% were above the bottom of vertebra L3. Horizontally, 93.33% of the LNs in the celiac level were located within an expansion of 1.5 cm on the CA, and 94.12% of the LNs in the superior mesenteric artery (SMA) level were within 1.5 cm on the left side of the SMA. Below the SMA, all the LNs were behind the left renal vein, left to the right border of the inferior vena cava, and 98.51% of the LNs were medial to the lateral surface of the left psoas major. The proposed CTV could cover 92.74% of the LNs and was dosimetrically feasible. The proposed CTV is the first one to focus on the high-risk area of abdominal failure in LTESCC patients after surgery and can serve as a reference in the adjuvant radiotherapy for LTESCC patients.
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http://dx.doi.org/10.7150/jca.51212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890317PMC
January 2021

Identification of a new orthonairovirus associated with human febrile illness in China.

Nat Med 2021 03 18;27(3):434-439. Epub 2021 Feb 18.

School of Life Sciences and Engineering, Foshan University, Foshan, Guangdong Province, China.

The genus Orthonairovirus, which is part of the family Nairoviridae, includes the important tick-transmitted pathogens Crimean-Congo hemorrhagic fever virus and Nairobi sheep disease virus, as well as many other poorly characterized viruses found in ticks, birds and mammals. In this study, we identified a new orthonairovirus, Songling virus (SGLV), from patients who reported being bitten by ticks in Heilongjiang Province in northeastern China. SGLV shared similar genomic and morphological features with orthonairoviruses and phylogenetically formed a unique clade in Tamdy orthonairovirus of the Nairoviridae family. The isolated SGLV induced cytopathic effects in human hepatoma cells in vitro. SGLV infection was confirmed in 42 hospitalized patients analyzed between 2017 and 2018, with the main clinical manifestations being headache, fever, depression, fatigue and dizziness. More than two-thirds (69%) of patients generated virus-specific antibody responses in the acute phase. Taken together, these results suggest that this newly discovered orthonairovirus is associated with human febrile illness in China.
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http://dx.doi.org/10.1038/s41591-020-01228-yDOI Listing
March 2021

Memory B Cell as an Indicator of Peri-implantitis Status: A Pilot Study.

Int J Oral Maxillofac Implants 2021 Jan-Feb;36(1):86-93

Purpose: Gingiva-resident memory B cells found recently in healthy periodontal tissue may play important roles in maintaining homeostasis against bacterial plaque. Whether resident memory B cells exist in healthy peri-implant tissue and how they respond in peri-implantitis lesions are of interest. The aim of this study was to preliminarily investigate whether memory B cell activities are related to inflamed or healthy peri-implant status.

Materials And Methods: Patients with peri-implantitis or healed implants were recruited. The gingiva samples were collected and divided into inflamed (n = 4), treated (n = 4), and healed (n = 3) groups, followed by a flow cytometry analysis staining with CD3, CD19, CD27, CD38, and RANKL. The biopsy samples were also cryo-embedded for immunofluorescent double staining of CD19 and CD27.

Results: CD27 CD38 ASC comprised 83.3% ± 3.3% of the total B cells in the inflamed group, and this proportion in the treated group was reduced to 44.5% ± 13.4%. The proportion of CD27 CD3 T cells was found to be unchanged between the inflamed and treated groups. Immunofluorescent staining indicated that CD19 CD27 population infiltrated peri-implant connective tissue. RANKL was expressed by almost all B cells and a portion of T cells in the inflamed group, while the proportions of RANKL B and T cells were significantly reduced in the treated group. Barely any memory B cells were detected in the healed group.

Conclusion: Memory B cells were markedly activated in peri-implantitis and responded to the suprastructure removal treatment. The lack of gingiva-resident memory B cells in the clinically healed implants serves as a hint for the weakness of peri-implant tissue against bacterial plaque.
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http://dx.doi.org/10.11607/jomi.8641DOI Listing
February 2021

Combination of bis (α-furancarboxylato) oxovanadium (IV) and metformin improves hepatic steatosis through down-regulating inflammatory pathways in high-fat diet-induced obese C57BL/6J mice.

Basic Clin Pharmacol Toxicol 2021 Feb 18. Epub 2021 Feb 18.

State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Key laboratory of Polymorphic Drugs of Beijing, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

The effects of the combination of bis (α-furancarboxylato) oxovanadium (IV) (BFOV) and metformin (Met) on hepatic steatosis were investigated in high-fat diet-induced obese C57BL/6J mice (HFC57 mice) for 6 weeks. Oral glucose tolerance test was performed to evaluate glucose metabolism. Moreover, blood and hepatic biochemical and histological indices were detected. Besides, Affymetrix-GeneChip analysis and Western blot of the liver were performed. Comparing to the monotherapy group, BFOV + Met showed more effective improvement in glucose metabolism, which decreased the fasting blood glucose, insulin levels and improved insulin sensitivity in HFC57 mice. BFOV + Met significantly decreased serum ALT and AST activities and reduced hepatic triglyceride content and iNOS activities, accompanied by ameliorating intrahepatic fat accumulation and hepatocellular vacuolation. Enhanced hepatic insulin signalling transduction and attenuated inflammation pathway were identified as the major pathways in the BFOV + Met group. BFOV + Met significantly down-regulated the protein expression levels of MMPs, NF-κB, iNOS and up-regulated phosphorylation of AKT and AMPK levels. We concluded that a combination of BFOV and metformin ameliorates hepatic steatosis in HFC57 mice via alleviating hepatic inflammation and enhancing insulin signalling pathway, suggesting that the combination of BFOV and metformin is a potential treatment for hepatic steatosis.
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http://dx.doi.org/10.1111/bcpt.13573DOI Listing
February 2021

Identification of MicroRNA-Potassium Channel Messenger RNA Interactions in the Brain of Rats With Post-traumatic Epilepsy.

Front Mol Neurosci 2020 1;13:610090. Epub 2021 Feb 1.

Hubei University of Police, Wuhan, China.

Dysregulated expression of microRNAs and potassium channels have been reported for their contributions to seizure onset. However, the microRNA-potassium channel gene interactions in traumatic brain injury-induced post-traumatic epilepsy (PTE) remain unknown. PTE was induced in male rats by intracranial injection with ferrous chloride (0.1 mol/L, 1 μl/min) at the right frontal cortex. Electroencephalography was recorded at 60 min, as well as day 1, 7, and 30, and the behavioral seizures were assessed before injection and at different time points after injection. Rats were killed on day 30 after injection. The right frontal cortex samples were collected and subjected to high throughput messenger RNA (mRNA) and microRNA sequencing. A network of differentially expressed potassium channel mRNAs and microRNAs was constructed using OryCun2.0 and subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. The differential mRNA and microRNA expressions were verified using quantitative real-time-PCR. The microRNA-mRNA was subject to the Pearson correlation analysis. A PTE rat model was successfully established, as evidenced by behavioral seizures and epileptiform discharges on electroencephalography in PTE rats compared with sham rats. Among the 91 mRNAs and 40 microRNAs that were significantly differentially expressed in the PTE rat brain, 4 mRNAs and 10 microRNAs were associated with potassium channels. Except for potassium calcium-activated channel subfamily N member 2, the other three potassium channel mRNAs were negatively correlated with seven microRNAs. These microRNA-mRNA pairs were enriched in annotations and pathways related to neuronal ion channels and neuroinflammation. Quantitative real-time-PCR and correlation analysis verified negative correlations in miR-449a-5p-KCNH2, miR-98-5p-KCNH2, miR-98-5p-KCNK15, miR-19b-3p-KCNK15, and miR-301a-3p-KCNK15 pairs. We identified microRNA-potassium channel mRNA interactions associated with PTE, providing potential diagnostic markers and therapeutic targets for PTE.
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http://dx.doi.org/10.3389/fnmol.2020.610090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882489PMC
February 2021

Risk of second primary malignancy after minor salivary gland cancer: A Surveillance, Epidemiology, and End Results database analysis.

Head Neck 2021 Feb 15. Epub 2021 Feb 15.

Department of Otolaryngology, Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai, China.

Background: Minor salivary gland cancer (MiSGC) is a group of tumors with varied disease course in the head and neck. We evaluated the risk of a second primary malignancy (SPM) in MiSGC patients and identified possible prognostic factors for survival using a large population database.

Methods: We used the National Cancer Institute's Surveillance, Epidemiology, and End Results (SEER) data to evaluate the risk and prognosis of SPM in patients diagnosed with MiSGC.

Results: The risk of SPM increased in MiSGC patients compared with the endemic rate. The risk of SPM was slightly greater in female patients and who underwent radiotherapy. Age at primary diagnosis, sex, race, year of diagnosis, SEER stage, radiotherapy, SPM, histology, and tumor site were significant survival prognostic indicators of MiSGC patients.

Conclusion: Radiotherapy and female sex were risk factors for SPM after MiSGC. Long-term surveillance for SPM was important in MiSGC patients.
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http://dx.doi.org/10.1002/hed.26641DOI Listing
February 2021

Types of Transnasal Endoscopic Nasopharyngectomy for Recurrent Nasopharyngeal Carcinoma: Shanghai EENT Hospital Experience.

Front Oncol 2020 14;10:555862. Epub 2021 Jan 14.

Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Medical College of Fudan University, Shanghai, China.

Background: Transnasal endoscopic nasopharyngectomy (TEN) has become increasingly used for recurrent nasopharyngeal carcinoma (rNPC); however, there is no report on the definitive resectable contour for TEN according to the latest staging system for nasopharyngeal carcinoma. The aim of this study was to establish the types of TEN for rNPC.

Materials And Methods: A total of 101 rNPC patients underwent TEN from January 2016 to April 2019 at the authors' institution. TEN was categorized into four types, which included type I (n=40) with resection of the nasopharynx and sinuses; type II (n=10) with lateral extension to the parapharyngeal space; type III (n=40) with lateral extension to the floor of the middle cranial fossa and the infratemporal fossa and superior extension to the orbital apex and the cavernous sinus back to the prevertebral region; and type IV (n=11) with the resection of the involved internal carotid artery following type III. The 2-year overall survival rate (OS) and local recurrence-free survival rate (LRFS) were assessed.

Results: The median time of follow-up was 20 months. Twenty-five patients reoccurred. Nineteen patients died. Independent predictors of outcome on multivariate analysis were recurrent T stage (P = 0.039), types of TEN (P = 0.002) and surgical margin (P = 0.003). The 2-year OS and LRFS was 76.2% and 53.6%, respectively.

Conclusions: The result of TEN in the treatment of rNPC is promising. The types of TEN will provide effective guideline for surgical treatment of rNPC.
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http://dx.doi.org/10.3389/fonc.2020.555862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873878PMC
January 2021

First detection of Borrelia miyamotoi infections in ticks and humans from the northeast of Inner Mongolia, China.

Acta Trop 2021 May 11;217:105857. Epub 2021 Feb 11.

College of Veterinary Medicine, Northeast Agricultural University, Changjiang Road No.600, Harbin 150030, Heilongjiang Province, China. Electronic address:

Borrelia miyamotoi is a relapsing fever spirochete that can cause chills, fatigue, headache, myalgia, arthralgia, and even meningitis, damaging human health. B. miyamotoi has a wide distribution since its discovery in Ixodes persulcatus in 1994. The human B. miyamotoi disease was first described in Russia in 2011. However, the epidemiological information in China is limited. Here, we report the molecular detection of B. miyamotoi in the northeast of Inner Mongolia, China. A total of 774 adult ticks and 771 blood samples of patients were collected, from April 2017 to August 2019 in the northeast of Inner Mongolia, and tested for B. miyamotoi using real time-PCR. Gene sequences of 16S rRNA, fla, and glpQ were obtained to reconstruct the phylogenetic relationship of B. miyamotoi from humans and ticks. The results showed the total prevalence of B. miyamotoi in ticks was 1.3% of 774 ticks, with rates of 2.6% in I. persulcatus, 0.78% in Dermacentor nuttalli, 1.3% in D. silvarum, and 0.4% in Haemaphysalis longicornis. Thirteen (1.7%) patients were confirmed as positive for B. miyamotoi. Patients were mainly 50-60-years old and had a history of tick contact. They presented flu-like symptoms, including fever, headache, poor spirit, dizziness, nausea, vomiting, hypodynamic, chest distress, and myalgia. Phylogenetic analysis showed that the B. miyamotoi in the present study belonged to the Siberian type, distinct from European and American types and the I. ovatus isolate from Japan. This is the first report of B. miyamotoi detection in both ticks and humans in the northeast of Inner Mongolia, China, indicating B. miyamotoi is present in the area. These findings suggest that people have a risk of infection with B. miyamotoi in this region, where it should be included the differential diagnosis of tick-borne diseases.
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http://dx.doi.org/10.1016/j.actatropica.2021.105857DOI Listing
May 2021

AI applications in renal pathology.

Kidney Int 2021 Feb 10. Epub 2021 Feb 10.

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, USA. Electronic address:

The explosive growth of artificial intelligence (AI) technologies, especially deep learning methods, has been translated at revolutionary speed to efforts in AI-assisted healthcare. New applications of AI to renal pathology have recently become available, driven by the successful AI deployments in digital pathology. However, synergetic developments of renal pathology and AI require close interdisciplinary collaborations between computer scientists and renal pathologists. Computer scientists should understand that not every AI innovation is translatable to renal pathology, while renal pathologists should capture high-level principles of the relevant AI technologies. Herein, we provide an integrated review on current and possible future applications in AI-assisted renal pathology, by including perspectives from computer scientists and renal pathologists. First, the standard stages, from data collection to analysis, in full-stack AI-assisted renal pathology studies are reviewed. Second, representative renal pathology-optimized AI techniques are introduced. Last, we review current clinical AI applications, as well as promising future applications with the recent advances in AI.
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http://dx.doi.org/10.1016/j.kint.2021.01.015DOI Listing
February 2021

-HSP20 Is Associated With the Infectivity and Encystation of .

Front Microbiol 2020 12;11:595080. Epub 2021 Jan 12.

Department of Pathogenic Biology, Jilin Medical University, Jilin City, China.

is a pathogenic and opportunistic free-living amoeba that causes keratitis (AK) and granulomatous amebic encephalitis (GAE) in immunocompromised individuals. The biological and pathogenic characterizations behind this opportunistic protozoan is not fully understood. This study aimed to determine the biological functions of heat shock protein (HSP)-20 of (-HSP20) involved in the maintenance of life cycle and the infectivity of . Immunoscreening cDNA library with infected rabbit sera identified three positive clones, one of them was a putative heat shock protein (-HSP20). The recombinant 23 kDa -HSP20 protein (r-HSP20) was successfully expressed in BL21 (DE3) and purified using metal affinity chromatography. The rabbits immunized with r-HSP20 produced high titer antibody (1:25,600). Immunolocalization with the antibody identified the expression of native -HSP20 on the surface of both trophozoites and cysts. Further, Western blot with antibody identified that the expression of native Ac-HSP20 was 7.5 times higher in cysts than in trophozoites. Blocking -HSP20 on the membrane of trophozoites with specific antibody or silencing gene transcription by siRNA inhibited their transformation into cysts at the early stage but returned to normal at the late stage by stimulating the transcription of . Incubation of trophozoites with anti--HSP20 IgG increased macrophage-involved phagocytosis to the protozoa and inhibited trophozoite infectivity on the cornea of rabbits compared with that without antibody. Our study provides that -HSP20 is a surface antigen involved in the encystation and infectivity of and thus an important target for vaccine and drug development.
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http://dx.doi.org/10.3389/fmicb.2020.595080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835678PMC
January 2021

Human Tacheng Tick Virus 2 Infection, China, 2019.

Emerg Infect Dis 2021 Feb;27(2):594-598

We used metagenomic analysis to identify Tacheng tick virus 2 infection in a patient with a history of tick bite in northwestern China. We confirmed the virus with reverse transcription-PCR, virus isolation, and genomic analysis. We detected viral RNA in 9.6% of ticks collected from the same region.
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http://dx.doi.org/10.3201/eid2702.191486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853585PMC
February 2021

Tuning the electronic structure of layered vanadium pentoxide by pre-intercalation of potassium ions for superior room/low-temperature aqueous zinc-ion batteries.

Nanoscale 2021 Feb;13(4):2399-2407

School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China.

Aqueous zinc-ion batteries (ZIBs), due to their sluggish Zn2+ diffusion kinetics, continue to face challenges in terms of achieving superior high rate, long-term cycling and low-temperature properties. Herein, K+ pre-intercalated layered V2O5 (K0.5V2O5) composites with metallic features are capable of delivering excellent zinc storage performance. Specifically, the K0.5V2O5 electrode delivers a high reversible capacity of 251 mA h g-1 at 5 A g-1 after 1000 cycles. Even at a low temperature of -20 °C, high reversible capacities of 241 and 115 mA h g-1 can be obtained after 1000 cycles at 1 and 5 A g-1, respectively. The outstanding electrochemical performance is attributed to the incorporation of K+ into the layered V2O5, which acts as pillars to promote the Zn2+ diffusion and increase the structural stability during cycling. Density functional theory calculations demonstrate that the interlayer doping of K+ can benefit electron migration, and therefore enhance the Zn2+ (de)intercalation kinetics. Meanwhile, the Zn2+ storage mechanism of K0.5V2O5 is revealed by ex situ X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy and transmission electron microscopy characterization. This work may pave the way for exploiting high-performance cathodes for aqueous ZIBs.
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http://dx.doi.org/10.1039/d0nr07358jDOI Listing
February 2021

Seraprevir and sofosbuvir for treatment of chronic hepatitis C virus infection: A single-arm, open-label, phase 3 trial.

J Gastroenterol Hepatol 2021 Jan 24. Epub 2021 Jan 24.

Department of Hepatology, The First Hospital of Jilin University, Changchun, China.

Background And Aim: This single-arm, open-label, multicenter, phase 3 trial evaluated the efficacy and safety of seraprevir, an hepatitis C virus (HCV) nonstructural protein 3/4A (NS3/4A) inhibitor, combined with sofosbuvir for treating Chinese patients with chronic HCV infection without cirrhosis.

Methods: Treatment-naive or interferon-experienced adult patients without cirrhosis were treated with a universal, combinational regimen of seraprevir 100 mg, twice daily and sofosbuvir 400 mg, once daily, for 12 or 24 weeks. The primary efficacy endpoint was sustained virologic response at week 12 after treatment (SVR12).

Results: Overall, 205 patients with genotype 1 HCV infection without cirrhosis were enrolled from 23 sites, 202 of whom completed the full treatment and post-treatment course and 3 discontinued follow-up. In total, 27 patients (13.2%) were interferon experienced. SVR12 was achieved by 201 out of 205 (98.0% [95% CI, 95.1%, 99.5%]) patients, 100.0% of patients with genotype 1a, and 98.0% of genotype 1b. In the other exploratory study, SVR 12 was achieved by 100% patients with genotype 2 (n = 21), genotype 3 (n = 7), and genotype 6 (n = 8). The majority of adverse events were mild to moderate and transient and did not require a specific medical intervention.

Conclusions: The all-oral, ribavirin-free regimen of seraprevir and sofosbuvir is an effective and well-tolerated treatment option for Chinese patients mono-infected with HCV, including those with a history of interferon treatment.
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http://dx.doi.org/10.1111/jgh.15412DOI Listing
January 2021

Direct Observation of Structural Heterogeneity and Tautomerization of Single Hypericin Molecules.

J Phys Chem Lett 2021 Jan 20;12(3):1025-1031. Epub 2021 Jan 20.

Institute of Physical and Theoretical Chemistry, Eberhard Karls University Tübingen, Auf der Morgenstelle 18, 72076 Tübingen, Germany.

Tautomerization is a fundamental chemical reaction which involves the relocation of a proton in the reactants. Studying the optical properties of tautomeric species is challenging because of ensemble averaging. Many molecules, such as porphines, porphycenes, or phenanthroperylene quinones, exhibit a reorientation of the transition dipole moment (TDM) during tautomerization, which can be directly observed in single-molecule experiments. Here, we study single hypericin molecules, which is a prominent phenanthroperylene quinone showing antiviral, antidepressive, and photodynamical properties. Observing abrupt flipping of the image pattern combined with time-dependent density functional theory calculations allows drawing conclusions about the coexistence of four tautomers and their conversion path. This approach allows the unambiguous assignment of a TDM orientation to a specific tautomer and enables the determination of the chemical structure . Our approach can be applied to other molecules showing TDM reorientation during tautomerization, helping to gain a deeper understanding of this important process.
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http://dx.doi.org/10.1021/acs.jpclett.0c03459DOI Listing
January 2021

The genetic variations in the mitochondrial genomes of three Luciolinae fireflies.

Authors:
Quan Liu Xinhua Fu

Mitochondrial DNA B Resour 2020 Aug 26;5(3):3210-3214. Epub 2020 Aug 26.

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.

This paper studied the complete mitochondrial genomes of three fireflies, and . We discussed the variations in the mitochondrial genomes of samples of each firefly from different populations. The mitochondrial genomes of and are very stable among their different populations. The mitochondrial genome of shows some variations among the different populations, especially in the COI sequence.
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http://dx.doi.org/10.1080/23802359.2020.1806126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783030PMC
August 2020

FGF10 Attenuates Experimental Traumatic Brain Injury through TLR4/MyD88/NF-κB Pathway.

Cells Tissues Organs 2021 Jan 13:1-9. Epub 2021 Jan 13.

Department of Neurosurgery, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou City, China.

Traumatic brain injury (TBI) can induce neuronal apoptosis and neuroinflammation, resulting in substantial neuronal damage and behavioral disorders. Fibroblast growth factors (FGFs) have been shown to be critical mediators in tissue repair. However, the role of FGF10 in experimental TBI remains unknown. In this study, mice with TBI were established via weight-loss model and validated by increase of modified neurological severity scores (mNSS) and brain water content. Secondly, FGF10 levels were elevated in mice after TBI, whereas intraventricular injection of Ad-FGF10 decreased mNSS score and brain water content, indicating the remittance of neurological deficit and cerebral edema in TBI mice. In addition, neuronal damage could also be ameliorated by stereotactic injection of Ad-FGF10. Overexpression of FGF10 increased protein expression of Bcl-2, while it decreased Bax and cleaved caspase-3/PARP, and improved neuronal apoptosis in TBI mice. In addition, Ad-FGF10 relieved neuroinflammation induced by TBI and significantly reduced the level of interleukin 1β/6, tumor necrosis factor α, and monocyte chemoattractant protein-1. Moreover, Ad-FGF10 injection decreased the protein expression level of Toll-like receptor 4 (TLR4), MyD88, and phosphorylation of NF-κB (p-NF-κB), suggesting the inactivation of the TLR4/MyD88/NF-κB pathway. In conclusion, overexpression of FGF10 could ameliorate neurological deficit, neuronal apoptosis, and neuroinflammation through inhibition of the TLR4/MyD88/NF-κB pathway, providing a potential therapeutic strategy for brain injury in the future.
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http://dx.doi.org/10.1159/000511381DOI Listing
January 2021