Publications by authors named "Quan Li"

966 Publications

Stimuli-Driven Insulator-Conductor Transition in a Flexible Polymer Composite Enabled by Biphasic Liquid Metal.

Adv Mater 2021 Sep 19:e2104634. Epub 2021 Sep 19.

Institute of Advanced Materials, School of Chemistry and Chemical Engineering, and Jiangsu Hi-Tech Key Laboratory for Biomedical Research, Southeast University, Nanjing, 211189, China.

Metal-polymer composites (MPCs) with combined properties of metals and polymers have achieved much industrial success. However, metals in MPCs are thought to be ordinary and invariable electrically conductive fillers in supportive polymers to show limited use in modern technologies. This work that is disclosed here, for the first time, introduces stimuli-driven transition from biphasic to monophasic state of liquid metal into polymer science to form dynamic soft conductors from the binary metal-polymer composites. The binary metal that exhibits temperature-driven reversible transition between solid and liquid states via a biphasic state is fabricated. A conducting stretchable polymer composite is developed using the judiciously chosen biphasic binary metal that undergoes conductor to insulator transition upon stretching. Insulating stretched films become conducting upon heating. A "tube" model elegantly describes such distinctive deformation/temperature-dependent behaviors. Moreover, the conducting polymer composite shows decrease in its resistance upon increasing the sample temperature. The resistance can be tuned from 1 to 10  Ω depending on the state of binary metal in the phase diagram. This work would build the intimate and interesting connection between metal phases and polymer science toward next-generation soft conductors and beyond.
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http://dx.doi.org/10.1002/adma.202104634DOI Listing
September 2021

Functional diversity loss and change in nocturnal behavior of mammals under anthropogenic disturbance.

Conserv Biol 2021 Sep 17. Epub 2021 Sep 17.

State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.

In the Anthropocene epoch, understanding the impacts of anthropogenic influence on biodiversity and behavior of vulnerable wildlife communities is increasingly relevant to effective conservation. However, comparative studies aimed at disentangling the concurrent effect of different types of human disturbance on multifaceted biodiversity and on activity patterns of mammals are surprisingly rare. We applied a multi-region community model to separately estimate the effects of cumulative human modification (settlement, agriculture, and transportation etc.) and human presence (aggregated presence of dogs, people and livestock on camera) on species richness and functional composition of medium- and large-bodied mammals based on camera trap data collected across 45 subtropical montane forests. We divided the detected mammal species into three trophic guilds: carnivores, herbivores and omnivores and assessed the nocturnal shifts of each guild in response to anthropogenic activities. Our results suggest that human modification and human presence had differing effects on mammals. Overall species richness tended to increase with human modification but decrease with human presence. Human modification was associated with significantly lower functional diversity, community average body mass and proportion of carnivores. Human presence was associated with a strongly reduced proportion of herbivores. The proportion of omnivores significantly increased with both habitat modification and human presence. In terms of activity patterns, omnivores and carnivores became more nocturnal in response to human modification. Our results demonstrate that anthropogenic disturbances can lead to drastic loss of functional diversity and result in a shift to nocturnal behavior of mammals. Conservation planning should consider concurrent effects of different types of human disturbance on wildlife communities, and take into account not only species richness, but also functional diversity and animal behavior. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/cobi.13839DOI Listing
September 2021

Comprehensive Identification of Liquid Crystal Monomers-Biphenyls, Cyanobiphenyls, Fluorinated Biphenyls, and their Analogues-in Waste LCD Panels and the First Estimate of their Global Release into the Environment.

Environ Sci Technol 2021 Sep 10. Epub 2021 Sep 10.

Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Guangdong-Hongkong-Macau Joint Laboratory of Collaborative Innovation for Environmental Quality, Jinan University, Guangzhou 511443, China.

Our previous study demonstrated massive emissions of liquid crystal monomers (LCMs) from liquid crystal display (LCD)-associated e-waste dismantling; however, the compositions, priority list, and inventory of LCMs in waste LCD panels remain unknown. Herein, we conducted the first comprehensive identification covering a broader range of LCMs, including 21 biphenyls and analogues (BAs), 28 cyanobiphenyls and analogues (CBAs), and 44 fluorinated biphenyls and analogues (FBAs), in waste television/computer LCD panels. A total of 64 of the 93 target LCMs, including 19 BAs, 6 CBAs, and 39 FBAs, were widely detected in collected waste LCD panels. Approximately 10-18 of the 64 detectable LCMs were identified as the main compositions in various waste LCD panels, which contributed to >90% of the total LCMs. Total concentrations of FBAs in the television/computer LCD panel samples were comparable to those of BAs but much higher than those of CBAs, indicating FBAs and BAs being the commonly used LCM categories. The composition distribution of LCMs varied between television/computer LCDs and among different brands of television/computer LCDs. A preliminary estimate of the globally direct release of LCMs from waste television/computer LCD panels into various environmental compartments was about 1.07-107 kg/year, which will increase considerably in the near future.
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http://dx.doi.org/10.1021/acs.est.1c03901DOI Listing
September 2021

Traditional herbal medicine and nanomedicine: Converging disciplines to improve therapeutic efficacy and human health.

Adv Drug Deliv Rev 2021 Sep 6;178:113964. Epub 2021 Sep 6.

Jiangsu Engineering Research Center for Efficient Delivery System of TCM, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China. Electronic address:

Traditional herbal medicine (THM), an ancient science, is a gift from nature. For thousands of years, it has helped humans fight diseases and protect life, health, and reproduction. Nanomedicine, a newer discipline has evolved from exploitation of the unique nanoscale morphology and is widely used in diagnosis, imaging, drug delivery, and other biomedical fields. Although THM and nanomedicine differ greatly in time span and discipline dimensions, they are closely related and are even evolving toward integration and convergence. This review begins with the history and latest research progress of THM and nanomedicine, expounding their respective developmental trajectory. It then discusses the overlapping connectivity and relevance of the two fields, including nanoaggregates generated in herbal medicine decoctions, the application of nanotechnology in the delivery and treatment of natural active ingredients, and the influence of physiological regulatory capability of THM on the in vivo fate of nanoparticles. Finally, future development trends, challenges, and research directions are discussed.
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http://dx.doi.org/10.1016/j.addr.2021.113964DOI Listing
September 2021

An enamel-inspired bioactive material with multiscale structure and antibacterial adhesion property.

Bioact Mater 2022 Jan 2;7:491-503. Epub 2021 Jun 2.

Collage and Hospital of Stomatology, Anhui Medical University, No. 69, Meishan Road, Heifei, China.

Conventional dental materials lack of the hierarchical architecture of enamel that exhibits excellent intrinsic-extrinsic mechanical properties. Moreover, restorative failures frequently occur due to physical and chemical mismatch between artificial materials and native dental hard tissue followed by recurrent caries which is caused by sugar-fermenting, acidogenic bacteria invasion of the defective cite. In order to resolve the limitations of the conventional dental materials, the aim of this study was to establish a non-cell-based biomimetic strategy to fabricate a novel bioactive material with enamel-like structure and antibacterial adhesion property. The evaporation-based, bottom-up and self-assembly method with layer-by-layer technique were used to form a large-area fluorapatite crystal layer containing antibacterial components. The multilayered structure was constructed by hydrothermal growth of the fluorapatite crystal layer and highly conformal adsorption to the crystal surface of a polyelectrolyte matrix film. Characterization and mechanical assessment demonstrated that the synthesized bioactive material resembled the native enamel in chemical components, mechanical properties and crystallographic structure. Antibacterial and cytocompatibility evaluation demonstrated that this material had the antibacterial adhesion property and biocompatibility. In combination with the molecular dynamics simulations to reveal the effects of variables on the crystallization mechanism, this study brings new prospects for the synthesis of enamel-inspired materials.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379364PMC
January 2022

Fabrication of in situ ZIF-67 grown on alginate hydrogels and its application for enhancing Cu (II) adsorption from aqueous solutions.

Colloids Surf B Biointerfaces 2021 Aug 14;207:112036. Epub 2021 Aug 14.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China. Electronic address:

Synthesizing high uniform metal-organic frameworks grown on natural biomass such as sodium alginate (ALG), which can efficiently remove metal ions from aqueous solution, is a challenging project. A simple and eco-friendly method of preparing [email protected] hydrogels by in situ synthesis of ZIF-67 onto ALG hydrogels was established. Combining the virtues of ALG with abundant hydroxyl and carboxyl groups and ZIF-67 with excellent porous structure, the obtained [email protected] hydrogels have excellent adsorption for Cu (153.63 mg/g), which was much higher than that of pure ALG hydrogels (111.79 mg/g). The methodology showed that the adsorption process was in accord with the Langmuir isotherm adsorption model (R = 0.9972) and pseudo-second-order kinetic model (R = 0.9822). The thermodynamic experiments demonstrated that Cu (II) was absorbed by hydrogel with an endothermic and spontaneous process. The competitive adsorption experiment results of [email protected] hydrogel indicated that the absorption capacity of Cu (II) was higher than that of Cd (II), Ni (II), Zn (II) and Mn (II). Moreover, the [email protected] hydrogels still had excellent reusability after five adsorption-desorption cycles. The results indicated that the [email protected] hydrogels had the advantages of low cost, easy preparation, and environment friendly.
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http://dx.doi.org/10.1016/j.colsurfb.2021.112036DOI Listing
August 2021

Light-fueled transient supramolecular assemblies in water as fluorescence modulators.

Nat Commun 2021 08 17;12(1):4993. Epub 2021 Aug 17.

Institute of Advanced Materials, School of Chemistry and Chemical Engineering, and Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, Southeast University, Nanjing, China.

Dissipative self-assembly, which requires a continuous supply of fuel to maintain the assembled states far from equilibrium, is the foundation of biological systems. Among a variety of fuels, light, the original fuel of natural dissipative self-assembly, is fundamentally important but remains a challenge to introduce into artificial dissipative self-assemblies. Here, we report an artificial dissipative self-assembly system that is constructed from light-induced amphiphiles. Such dissipative supramolecular assembly is easily performed using protonated sulfonato-merocyanine and chitosan based molecular and macromolecular components in water. Light irradiation induces the assembly of supramolecular nanoparticles, which spontaneously disassemble in the dark due to thermal back relaxation of the molecular switch. Owing to the presence of light-induced amphiphiles and the thermal dissociation mechanism, the lifetimes of these transient supramolecular nanoparticles are highly sensitive to temperature and light power and range from several minutes to hours. By incorporating various fluorophores into transient supramolecular nanoparticles, the processes of aggregation-induced emission and aggregation-caused quenching, along with periodic variations in fluorescent color over time, have been demonstrated. Transient supramolecular assemblies, which act as fluorescence modulators, can also function in human hepatocellular cancer cells.
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http://dx.doi.org/10.1038/s41467-021-25299-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371092PMC
August 2021

Mechanical Ventilation Exacerbates Poly (I:C) Induced Acute Lung Injury: Central Role for Caspase-11 and Gut-Lung Axis.

Front Immunol 2021 19;12:693874. Epub 2021 Jul 19.

Department of Anesthesiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, China.

Background: The mechanisms by which moderate tidal volume ventilation (MTV) exacerbates preexisting lung injury are unclear. We hypothesized that systemic endotoxemia the gut-lung axis would lead to non-canonical and canonical inflammasome activation and pyroptosis in a two-hit model involving polyinosinic-polycytidylic acid (Poly(I:C)), a synthetic analog of dsRNA and MTV and that this would associate with acute lung injury (ALI).

Methods: Anesthetized mice were administered Poly(I:C) intratracheally and then 6 h later, they were mechanically ventilated for 4 h with otherwise non-injurious MTV (10ml/kg). Changes in intestinal and alveolar capillary permeability were measured. Further documentation of ALI was assessed by evans blue albumin permeability, protein and IL-1 family concentration in bronchoalveolar lavage fluid (BALF) or plasma, and histopathology in cohorts of wildtype (WT), whole body genetically ablated caspase-11 (caspase-11), caspase-1/caspase-11 double knockout (caspase-1/11), gasdermin D (GSDMD), nucleotide-binding domain leucine-rich repeat-containing protein 3 (NLRP3) and advanced glycosylation end product-specific receptor (RAGE) mice.

Results: Non-injurious MTV exacerbated the mild lung injury associated with Poly(I:C) administration. This included the disruption of alveolar-capillary barrier and increased levels of interleukin (IL)-6, high mobility group proteins 1 (HMGB-1), IL-1β in BALF and IL-18 in plasma. Combined (Poly(I:C)-MTV) injury was associated with increase in gastrointestinal permeability and endotoxin in plasma and BALF. Poly(I:C)-MTV injury was sensitive to caspase-11 deletion with no further contribution of caspase-1 except for maturation and release of IL-18 (that itself was sensitive to deletion of NLRP3). Combined injury led to large increases in caspase-1 and caspase-11. Genetic ablation of GSDMD attenuated alveolar-capillary disruption and release of cytokines in combined injury model.

Conclusions: The previously noted exacerbation of mild Poly(I:C)-induced ALI by otherwise non-injurious MTV is associated with an increase in gut permeability resulting in systemic endotoxemia. The gut-lung axis resulted in activation of pulmonary non-canonical (cytosolic mediated caspase-11 activation) and canonical (caspase-1) inflammasome (NLRP3) mediated ALI in this two-hit model resulting in GSDMD sensitive alveolar capillary barrier disruption, pyroptosis (alveolar macrophages) and cytokine maturation and release (IL-1β; IL-18). Pharmacologic strategies aimed at disrupting communication between gut and lung, inhibition of inflammasomes or GSDMD in pyroptosis may be useful in ALI.
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http://dx.doi.org/10.3389/fimmu.2021.693874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327178PMC
July 2021

The Composite of 3, 4-Dihydroxyl-Phenyl Lactic Acid and Notoginsenoside R1 Attenuates Myocardial Ischemia and Reperfusion Injury Through Regulating Mitochondrial Respiratory Chain.

Front Physiol 2021 12;12:538962. Epub 2021 Jul 12.

Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China.

Aim: 3,4-Dihydroxyl-phenyl lactic acid (DLA) and notoginsenoside R1 (R1) are known to protect ischemia and reperfusion (I/R) injury by targeting Sirtuin1/NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 10/the Mitochondrial Complex I (Sirt-1/NDUFA10/Complex I) and Rho-associated kinase/adenosine triphosphate (ROCK/ATP) ATP synthase δ subunit (ATP 5D), respectively. We hypothesized that a composite of the two may exhibit a more potent effect on I/R injury. The study was designed to test this hypothesis.

Materials And Methods: Male Sprague-Dawley rats underwent left anterior descending artery occlusion and reperfusion, with or without DLA, R1, or a combination of 3,4-dihydroxyl-phenyl lactic acid and notoginsenoside R1 (DR) pretreatment. Heart function, myocardial morphology, myocardial infarct, myocardial blood flow (MBF), apoptosis, vascular diameter, and red blood cell (RBC) velocity in venules were evaluated. Myeloperoxidase (MPO), malondialdehyde (MDA), and 8-oxo-deoxyguanosine (8-OHdG) were assessed. The content of ATP, adenosine diphosphate (ADP), and adenosine monophosphate (AMP), the activity of mitochondrial respiratory chain Complex I and its subunit NDUFA10, the Mitochondrial Complex V (Complex V) and its subunit ATP 5D, Sirt-1, Ras homolog gene family, member A (RhoA), ROCK-1, and phosphorylated myosin light chain (P-MLC) were evaluated. R1 binding to Sirt-1 was determined by surface plasmon resonance.

Results: DLA inhibited the expression of Sirt-1, the reduction in Complex I activity and its subunit NDUFA10 expression, the increase in MPO, MDA, and 8-OhdG, and apoptosis. R1 inhibited the increase in the expression of RhoA/ROCK-1/P-MLC, the reduction of Complex V activity and its subunit ATP 5D expression, alleviated F-actin, and myocardial fiber rupture. Both DLA and R1 reduced the myocardial infarction size, increased the velocities of RBC in venules, and improved MBF and heart function impaired by I/R. DR exhibited effects similar to what was exerted, respectively, by DLA and R1 in terms of respiratory chain complexes and related signaling and outcomes, and an even more potent effect on myocardial infarct size, RBC velocity, heart function, and MBF than DLA and R1 alone.

Conclusion: A combination of 3,4-dihydroxyl-phenyl lactic acid and notoginsenoside R1 revealed a more potent effect on I/R injury via the additive effect of DLA and R1, which inhibited not only apoptosis caused by low expression of Sirt-1/NDUFA10/Complex I but also myocardial fiber fracture caused by RhoA/ROCK-1 activation and decreased expression of ATP/ATP 5D/Complex V.
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http://dx.doi.org/10.3389/fphys.2021.538962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311465PMC
July 2021

Antitumor efficacy of XPO1 inhibitor Selinexor in KRAS-mutant lung adenocarcinoma patient-derived xenografts.

Transl Oncol 2021 Oct 17;14(10):101179. Epub 2021 Jul 17.

Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto, Ontario M5G 1L7, Canada. Electronic address:

Gain-of-function Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations occur in 25% of lung adenocarcinomas, and these tumors are challenging to treat. Some preclinical work, largely based on cell lines, suggested KRAS lung cancers are especially dependent on the nuclear export protein exportin-1 (XPO1), while other work supports XPO1 being a broader cancer dependency. To investigate the sensitivity of KRAS lung cancers to XPO1 inhibition in models that more closely match clinical tumors, we treated 10 independently established lung cancer patient-derived tumor xenografts (PDXs) with the clinical XPO1 inhibitor, Selinexor. Monotherapy with Selinexor reduced tumor growth in all KRAS PDXs, which included 4 different codon mutations, and was more effective than the clinical MEK1/2 inhibitor, Trametinib. Selinexor was equally effective in KRAS and KRAS tumors, with TP53 mutations being a biomarker for a weaker drug response. By mining genome-wide dropout datasets, we identified XPO1 as a universal cancer cell dependency and confirmed this functionally in two KRAS PDX models harboring kinase drivers. However, targeted kinase inhibitors were more effective than Selinexor in these models. Our findings support continued investigation of XPO1 inhibitors in KRAS lung adenocarcinoma, regardless of the codon alteration.
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http://dx.doi.org/10.1016/j.tranon.2021.101179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313753PMC
October 2021

Covalent Adaptable Liquid Crystal Networks Enabled by Reversible Ring-Opening Cascades of Cyclic Disulfides.

J Am Chem Soc 2021 Aug 18;143(32):12543-12551. Epub 2021 Jul 18.

Institute of Advanced Materials, School of Chemistry and Chemical Engineering, and Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University, Nanjing 211189, China.

The development of covalent adaptable liquid crystal networks (LCNs) enabled by introducing dynamic covalent bonds has endowed liquid crystal actuators with self-healing properties and reversible shape programmability, broadening their applications in diverse soft robotic devices. However, the finite molecular design strategy limits the recyclability and the architectural diversity of these materials. Here, a strategy is first reported for fabricating photoresponsive polydisulfide-based covalent adaptable LCNs by ring-opening polymerization of cyclic dithiolane groups. Based on the disulfide metathesis, the resulting materials are self-healable, reshapable, and reprogrammable. Importantly, the equilibrium between the polymer backbones and the dithiolane-functionalized monomers enables catalytic depolymerization to recycle monomers, which could significantly weaken the disadvantage of subtractive manufacturing of photomechanical devices. This work rooted in chemistry would provide an economical and environmentally friendly strategy for the fabrication of functional soft robotics with excellent programmability and renewability and beyond.
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http://dx.doi.org/10.1021/jacs.1c03661DOI Listing
August 2021

Is Complete Correction of Cervical Sagittal Malalignment Necessary During 4-Level Anterior Cervical Discectomy and Fusion Surgery in Patients With Kyphosis?

Global Spine J 2021 Jul 15:21925682211031175. Epub 2021 Jul 15.

Department of Spine Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Study Design: Retrospective analysis.

Objective: We investigated whether complete correction of cervical sagittal malalignment is necessary during 4-level anterior cervical discectomy and fusion (ACDF) in patients with kyphosis.

Methods: This retrospective study included 84 patients who underwent 4-level ACDF surgery at a university hospital between January 2010 and December 2015. Based on the degree of cervical lordosis correction, patients were categorized into the following groups: mild (0-10°), moderate (10-20°), and complete correction (>20°). The clinical outcomes, radiological parameters, and functional outcomes were analyzed.

Results: We observed no significant intergroup differences in the baseline characteristics. The cervical sagittal vertical axis (CSVA) correction loss at the final follow-up was lesser in the mild- and moderate- than in the complete-correction group. The spinocranial angle (SCA) and T1 slope (T1 S) were significantly higher in the moderate- and complete-correction groups than in the mild-correction group, 3 days postoperatively. The cervical proximal junctional kyphosis (CPJK), adjacent segment degeneration (ASD), and ASD following CPJK rates were higher in the complete-correction group. We observed no significant intergroup differences in postoperative complications; however, 5 patients showed internal fixation failure in the complete-correction group; 4 of these patients required reoperation. No significant intergroup difference was observed in the Japanese Orthopedic Association and neck disability index scores at any time point.

Conclusions: A mild-to-moderate correction of cervical lordosis is superior to complete correction in patients with kyphosis who undergo 4-level ACDF because this approach is associated with lesser axial stress and CSVA correction loss.
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http://dx.doi.org/10.1177/21925682211031175DOI Listing
July 2021

Biochar Amendment Alters the Nutrient-Use Strategy of Moso Bamboo Under N Additions.

Front Plant Sci 2021 23;12:667964. Epub 2021 Jun 23.

State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.

Nutrient resorption can affect plant growth, litter decomposition, and nutrient cycling. Although the effects of nitrogen (N) and biochar fertilizers on soil nutrient concentrations and plant nutrient uptake have been studied, an understanding of how combined applications of N and biochar affect plant nutrient resorption in plantations is lacking. In this study, we applied N (0, 30, 60, and 90 kg N ha yr defined as N0, N30, N60, and N90, respectively) and biochar (0, 20, and 40 t biochar ha defined as BC0, BC20, and BC40, respectively) to the soil of a Moso bamboo plantation. We investigated the effects of these treatments on N and phosphorus (P) resorption by young and mature bamboo plants, as well as the relationships between nutrient resorption and leaf and soil nutrient concentrations. Young bamboo showed significantly greater foliar N resorption efficiency (NRE) and P resorption efficiency (PRE) than mature bamboo. N addition alone significantly increased the N resorption proficiency (NRP) and P resorption proficiency (PRP) but significantly decreased the NRE and PRE of both young and mature bamboo. In both the N-free and N-addition treatments, biochar amendments significantly reduced the foliar NRE and PRE of young bamboo but had the opposite effect on mature bamboo. Foliar NRE and PRE were significantly negatively correlated with fresh leaf N and P concentrations and soil total P concentration but significantly positively correlated with soil pH. Our findings suggest that N addition inhibits plant nutrient resorption and alters the nutrient-use strategy of young and mature bamboo from "conservative consumption" to "resource spending." Furthermore, biochar amendment enhanced the negative effect of N addition on nutrient resorption in young bamboo but reduced the negative effect on that of mature bamboo under N-addition treatments. This study provides new insights into the combined effects of N and biochar on the nutrient resorption of Moso bamboo and may assist in improving fertilization strategies in Moso bamboo plantations.
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http://dx.doi.org/10.3389/fpls.2021.667964DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261046PMC
June 2021

Job preferences of undergraduate pharmacy students in China: a discrete choice experiment.

Hum Resour Health 2021 Jul 6;19(1):79. Epub 2021 Jul 6.

Centre for Health Management and Policy Research, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China.

Background: Pharmacists are a crucial part of the health workforce and play an important role in achieving universal health coverage. In China, pharmaceutical human resources are in short supply, and the distribution is unequal. This study aimed to identify the key job characteristics that influence the job preferences of undergraduate pharmacy students and to elicit the relative importance of different job characteristics to shed light on future policy interventions.

Methods: A discrete choice experiment was conducted to assess the job preferences of undergraduate pharmacy students from 6 provinces in mainland China. A face-to-face interview was used to collect data. Conditional logit and mixed logit models were used to analyse data, and the final model was chosen according to the model fit statistics. A series of policy simulations was also conducted.

Results: In total, 581 respondents completed the questionnaire, and 500 respondents who passed the internal consistency test were analysed. All attributes were statistically significant except for open management. Monthly income and work location were most important to respondents, followed by work unit (which refers to the nature of the workplace) and years to promotion. There was preference heterogeneity among respondents, e.g., male students preferred open management, and female students preferred jobs in public institutions. Furthermore, students with an urban background or from a single-child family placed higher value on a job in the city compared to their counterparts.

Conclusion: The heterogeneity of attributes showed the complexity of job preferences. Both monetary and nonmonetary job characteristics significantly influenced the job preferences of pharmacy students in China. A more effective policy intervention to attract graduates to work in rural areas should consider both incentives on the job itself and the background of pharmacy school graduates.
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http://dx.doi.org/10.1186/s12960-021-00626-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259344PMC
July 2021

Risk factors for venous thromboembolism in patients with diabetes undergoing joint arthroplasty.

BMC Musculoskelet Disord 2021 Jul 6;22(1):608. Epub 2021 Jul 6.

Department of Spine Surgery, Beijing Jishuitan Hospital, No. 31, Xinjiekou East Street, Xicheng District, 100035, Beijing, People's Republic of China.

Background: Venous thromboembolism (VTE) is a significant complication after joint arthroplasty. Diabetes is related to a few changes in coagulation and fibrinolysis that may lead to thrombophilia. We aimed to investigate the incidence of postoperative VTE and associated risk factors among patients with diabetes undergoing total hip (THA) or total knee anthroplasty (TKA) in a single centre in China.

Methods: Patients with diabetes who underwent THA or TKA from January 2016 to December 2018 (n = 400) at Beijing Jishuitan Hospital were recruited in this study. Lower limb venous Doppler ultrasound was performed before and after surgery to confirm deep venous thrombosis (DVT). Computer tomography pulmonary angiography was done to confirm pulmonary embolism (PE) for those with new postoperative DVT and typical symptoms of PE. A multivariate logistic regression model was conducted to examine factors associated with the development of postoperative VTE.

Results: The overall incidence of postoperative VTE in patients with diabetes after THA or TKA was 46.8 % (187 out of 400). Among the 187 VTE patients, 7.5 % (14 out of 187) had proximal vein thrombosis and 92.5 % (173 out of 187) had distal vein thrombosis. No PE occurred. Female patients and patients undergoing TKA had higher incidence of postoperative VTE. Patients who developed postoperative VTE were older, and had higher levels of preoperative D-Dimer and Caprini score. A high level of preoperative D-dimer (OR = 2.11, 95 %CI = 1.35-3.30) and the surgery of TKA (OR = 2.29, 95 %CI = 1.29-4.01) significantly increased the risk of developing postoperative VTE. Postoperative initiation of concomitant mechanical prophylaxis and low molecular weight heparin (LMWH) was protective for postoperative VTE (OR = 0.56, 95 %CI = 0.37-0.86).

Conclusions: VTE is common in patients with diabetes undergoing joint arthroplasty. Patients undergoing TKA or with a high level of preoperative D-dimer are at a considerable risk of developing postoperative VTE. There may be a protective role of postoperative initiation of concomitant mechanical prophylaxis and LMWH for VTE.
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http://dx.doi.org/10.1186/s12891-021-04453-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261962PMC
July 2021

Schizandrin A ameliorates cognitive functions via modulating microglial polarisation in Alzheimer's disease mice.

Pharm Biol 2021 Dec;59(1):860-867

Hospital Office, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.

Context: Schizandrin A (Sch A) is a major phytochemical from (Turcz.) Baill. (Schisandraceae), which exerts a neuroprotective effect in Alzheimer's disease (AD).

Objective: To investigate the mechanism of Sch A in AD.

Materials And Methods: AD group: APP/PS1 transgenic mice served as AD models; AD + SCH group: APP/PS1 received 2 mg/kg Sch A by intragastric administration; WT: C57BL/6 mice were used as control. For assay, mouse microglial BV2 cells were treated with 0.5 µg/mL lipopolysaccharide or combined with 10 μmol/L Sch A for 24 h. The cognitive function and apoptosis in the mice was estimated. Microglial polarisation in the mice and cells was analysed.

Results: Sch A treatment effectively improved spatial learning and memory ability and suppressed apoptosis in the brain tissues of APP/PS1 mice. APP/PS1 mice exhibited an increase in the levels of Aβ1-42 (2367.9 ± 431.1 pg/mg) and Aβ1-40 (1753.3 ± 253.4 pg/mg), which was abolished by Sch A treatment. Moreover, Sch A treatment repressed the proportions of iNOS/Iba-1 cells and IL-6 expression, while enhanced the proportions of Arg-1/Iba-1 cells and IL-10 expression in APP/PS1 mice. , Sch A treatment reduced the proportions of CD16/32 cells, iNOS expression and IL-6 levels (25.7 ± 5.3 pg/mL) repressed M1 polarisation, and enhanced the proportions of CD206 cells, Arg-1 expression and IL-10 levels (75.9 ± 12.8 pg/mL) in BV2 cells.

Conclusions: This research confirms the neuroprotective effect of Sch A in AD, suggesting that Sch A may become a potential anti-AD agent.
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http://dx.doi.org/10.1080/13880209.2021.1941132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259827PMC
December 2021

OmpC, a novel factor H-binding surface protein, is dispensable for the adherence and virulence of Salmonella enterica serovar Typhimurium.

Vet Microbiol 2021 Aug 18;259:109157. Epub 2021 Jun 18.

College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China; Key Laboratory of Animal Infectious Diseases, Ministry of Agriculture, Yangzhou University, China; Jiangsu Key Laboratory of Preventive Veterinary Medicine, Yangzhou University, China. Electronic address:

Salmonella enterica serovar Typhimurium utilizes a series of strategies to evade host innate immune defenses, including the serum complement system. Many microbial pathogens have evolved the ability to bind the complement regulatory protein factor H (FH) through their surface factor H-binding proteins (FHBPs) to circumvent the complement-mediated bactericidal effect. However, the roles of FHBPs in Salmonella pathogenesis are not well understood. In this study, we demonstrated that the survival of S. Typhimurium in human serum was decreased in a time and concentration dependent manner. Pre-incubation with FH attenuated the sensitivity of S. Typhimurium strain χ3761 to complement-mediated serum killing, suggesting FH binding enhance survival in serum. We aimed to identify novel S. Typhimurium FHBPs and characterize their biological functions. Here, six potential FHBPs were identified by two-dimensional (2D)-Far-western blot, and three of them were further confirmed to bind FH by Far-western blot and dot blot. We found that deletion of ompC (ΔompC) significantly inhibited the survival of S. Typhimurium strain χ3761 in human serum. Our results indicated that the ompC mutation does not affect χ3761 adhesion to HeLa cells. Furthermore, a mice infection model showed that deletion of ompC had no significant effect on the histopathological lesions or viability compared with the wild-type strain χ3761. In summary, these results suggested that OmpC is an important FHBP, but not a critical virulence factor of S. Typhimurium.
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http://dx.doi.org/10.1016/j.vetmic.2021.109157DOI Listing
August 2021

Treatment of sickle cell disease by increasing oxygen affinity of hemoglobin.

Blood 2021 Jul 1. Epub 2021 Jul 1.

NIDDK NIH, Bethesda, Maryland, United States.

The issue of treating sickle cell disease with drugs that increase hemoglobin oxygen affinity has come to the fore with the FDA approval in 2019 of voxelotor, the only anti-sickling drug approved since hydroxyurea in 1998. Voxelotor reduces sickling by increasing the concentration of the non-polymerizing, high oxygen affinity R (oxy) conformation of HbS. Treatment of sickle cell patients with voxelotor increases Hb levels and decreases indicators of hemolysis, but with no indication as yet that it reduces the frequency of pain episodes. Here we use the allosteric model of Monod, Wyman, and Changeux to simulate whole blood oxygen dissociation curves and red cell sickling in the absence and presence of voxelotor under the in vivo conditions of rapid oxygen pressure decreases. Our modeling agrees with experiments using a new robust assay, which shows the very large, expected decrease in sickling from the drug. The modeling indicates, however, that the increase in oxygen delivery from reduced sickling is largely offset by the increase in oxygen affinity. The net result is that the drug increases overall oxygen delivery only at the very lowest oxygen pressures. Reduction of sickling does, however, mitigate against red cell damage and explains the observed decrease in hemolysis. More importantly, our modeling of in vivo oxygen dissociation, sickling, and oxygen delivery suggests that drugs that increase fetal hemoglobin or decrease MCHC, should be more therapeutically effective than drugs that increase oxygen affinity.
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http://dx.doi.org/10.1182/blood.2021012070DOI Listing
July 2021

Understanding water slippage through carbon nanotubes.

Phys Chem Chem Phys 2021 Jul;23(27):14737-14745

Key Laboratory of Automobile Materials (Jilin University), Ministry of Education, Department of Materials Science and Engineering, Jilin University, Changchun 130022, China.

It is a formidable challenge to understand water slippage through carbon nanotubes (CNTs), despite its great significance in fundamental research and technology. Herein, we propose an effective scheme to describe water slippage properties by extending two friction models - the phononic friction model and Einstein's diffusion model, both relying on the potential corrugation of water slippage. Our scheme effectively captures the tube-size effect on the viscosity and slippage of water molecules through CNTs. It also identifies the experimentally reported size-dependent transition from continuum to sub-continuum flow and further reveals that this transition is likely to be determined by the hydrogen bond instead of the structural transition or entropic change. Besides, the size-dependence of slip lengths is found to be controllable by temperature. Our methods are thus expected to be a useful basis for further studies on substance transport under confinement.
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http://dx.doi.org/10.1039/d1cp01148kDOI Listing
July 2021

A Novel Strategy for Caries Management: Constructing an Antibiofouling and Mineralizing Dual-Bioactive Tooth Surface.

ACS Appl Mater Interfaces 2021 Jul 22;13(26):31140-31152. Epub 2021 Jun 22.

Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Hong Kong SAR 999077, China.

Existing single-functional agents against dental caries are inadequate in antibacterial performance or mineralization balance. This problem can be resolved through a novel strategy, namely, the construction of an antibiofouling and mineralizing dual-bioactive tooth surface by grafting a dentotropic moiety to an antimicrobial peptide. The constructed bioactive peptide can strongly adsorb onto the tooth surface and has beneficial functions in a myriad of ways. It inhibits cariogenic bacteria adhesion, kills planktonic , and destroys the biofilm on the tooth surface. It also protects teeth from demineralization in acidic environments, and induces self-healing regeneration in the remineralization environment. Molecular dynamics simulations elucidate the main adsorption mechanism that the positively charged amino acid residues in the bioactive peptide bind to phosphate groups on the tooth surface, and the main mineralization mechanism that the negative charges on the outermost layer of the bioactive peptide repel acetic acid ions and attract calcium ions as nucleation sites for remineralization. This study suggests that this in-house synthesized dual-bioactive peptide is a promising functional agent to prevent dental caries, and is effective in inducing in situ self-healing remineralization for the treatment of decayed teeth.
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http://dx.doi.org/10.1021/acsami.1c06989DOI Listing
July 2021

State of the Art and Prospects for Halide Perovskite Nanocrystals.

ACS Nano 2021 Jul 17;15(7):10775-10981. Epub 2021 Jun 17.

Department of Materials Science and Engineering, and Centre for Functional Photonics (CFP), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong S.A.R.

Metal-halide perovskites have rapidly emerged as one of the most promising materials of the 21st century, with many exciting properties and great potential for a broad range of applications, from photovoltaics to optoelectronics and photocatalysis. The ease with which metal-halide perovskites can be synthesized in the form of brightly luminescent colloidal nanocrystals, as well as their tunable and intriguing optical and electronic properties, has attracted researchers from different disciplines of science and technology. In the last few years, there has been a significant progress in the shape-controlled synthesis of perovskite nanocrystals and understanding of their properties and applications. In this comprehensive review, researchers having expertise in different fields (chemistry, physics, and device engineering) of metal-halide perovskite nanocrystals have joined together to provide a state of the art overview and future prospects of metal-halide perovskite nanocrystal research.
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http://dx.doi.org/10.1021/acsnano.0c08903DOI Listing
July 2021

synthesis of fluorescent polydopamine polymer dots based on Fenton reaction for a multi-sensing platform.

J Mater Chem B 2021 Jul;9(27):5503-5513

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 3rd North East Road, Chaoyang District, Beijing 100029, China.

The development of fluorescent nanosensors has attracted extensive research interest owing to their superior optoelectronic properties. However, current fluorescent nanoprobes generally involve complicated synthesis processes, background signal disturbance, and limited analyte detection. In this work, a facile and time-saving synthetic strategy for the preparation of green emitting polydopamine polymer dots (PDA-PDs) from dopamine via Fenton reaction at room temperature was proposed for the first time. The obtained PDA-PDs possessed excellent luminescence properties, with a long-wavelength emission of 522 nm, a large Stokes shift of 142 nm, and good photostability against ionic strength and UV irradiation. The formation mechanism of fluorescent PDA-PDs is as follows: in the presence of Fe2+ and H2O2, dopamine could rapidly undergo oxidation to its quinone derivatives and further polymerize to synthesize the fluorescent PDA-PDs with the acceleration of hydroxyl radicals produced from the Fenton reaction. Thus, a versatile turn-on fluorescence sensing method was developed for the detection of multi-analytes (including Fe2+, dopamine, H2O2, and glucose) based on monitoring the intrinsic fluorescence signal of the in situ formation of PDA-PDs. This sensing method could be efficiently applied for the detection of Fe2+, dopamine, and glucose in real human serum samples. Moreover, a three-input AND molecular logic gate based on this sensing platform was designed with the fluorescence signal of PDA-PDs as the gate. Finally, the proposed PDA-PDs could have immense broad prospects in nanomaterials and biosensors.
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http://dx.doi.org/10.1039/d1tb00764eDOI Listing
July 2021

Vibrational relaxation dynamics in layered perovskite quantum wells.

Proc Natl Acad Sci U S A 2021 Jun;118(25)

Department of Chemistry, University of California, Berkeley, CA 94720;

Organic-inorganic layered perovskites, or Ruddlesden-Popper perovskites, are two-dimensional quantum wells with layers of lead-halide octahedra stacked between organic ligand barriers. The combination of their dielectric confinement and ionic sublattice results in excitonic excitations with substantial binding energies that are strongly coupled to the surrounding soft, polar lattice. However, the ligand environment in layered perovskites can significantly alter their optical properties due to the complex dynamic disorder of the soft perovskite lattice. Here, we infer dynamic disorder through phonon dephasing lifetimes initiated by resonant impulsive stimulated Raman photoexcitation followed by transient absorption probing for a variety of ligand substitutions. We demonstrate that vibrational relaxation in layered perovskite formed from flexible alkyl-amines as organic barriers is fast and relatively independent of the lattice temperature. Relaxation in layered perovskites spaced by aromatic amines is slower, although still fast relative to bulk inorganic lead bromide lattices, with a rate that is temperature dependent. Using molecular dynamics simulations, we explain the fast rates of relaxation by quantifying the large anharmonic coupling of the optical modes with the ligand layers and rationalize the temperature independence due to their amorphous packing. This work provides a molecular and time-domain depiction of the relaxation of nascent optical excitations and opens opportunities to understand how they couple to the complex layered perovskite lattice, elucidating design principles for optoelectronic devices.
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http://dx.doi.org/10.1073/pnas.2104425118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237612PMC
June 2021

A New Perspective and Design Principle for Halide Perovskites: Ionic Octahedron Network (ION).

Nano Lett 2021 Jun 13;21(12):5415-5421. Epub 2021 Jun 13.

Department of Chemistry, University of California, Berkeley, California 94720, United States.

The metal halide ionic octahedron, [MX] (M = metal cation, X = halide anion), is considered to be the fundamental building block and functional unit of metal halide perovskites. By representing the metal halide ionic octahedron in halide perovskites as a super ion/atom, the halide perovskite can be described as an extended ionic octahedron network (ION) charge balanced by selected cations. This new perspective of halide perovskites based on ION enables the prediction of different packing and connectivity of the metal halide octahedra based on different solid-state lattices. In this work, a new halide perovskite CsAuInCl was discovered on the basis of a BaTiO-lattice ION {[InCl][AuCl][Au/InCl]}, which is assembled from three different ionic octahedra [InCl], [AuCl], and [Au/InCl] and balanced by positively charged Cs cations. The success of this ION design concept in the discovery of CsAuInCl opens up a new venue for the rational design of new halide perovskite materials.
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http://dx.doi.org/10.1021/acs.nanolett.1c01897DOI Listing
June 2021

Fast Li Plating Behavior Probed by X-ray Computed Tomography.

Nano Lett 2021 06 9;21(12):5254-5261. Epub 2021 Jun 9.

Beijing Advanced Innovation Center for Materials Genome Engineering, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

Uneven lithium plating/stripping is an essential issue that inhibits stable cycling of a lithium metal anode and thus hinders its practical applications. The investigation of this process is challenging because it is difficult to observe lithium in an operating device. Here, we demonstrate that the microscopic lithium plating behavior can be observed in situ in a close-to-practical cell setup using X-ray computed tomography. The results reveal the formation of porous structure and its progressive evolution in space over the charging process with a large current. The elaborated analysis indicates that the microstructure of deposited lithium makes a significant impact on the subsequent lithium plating, and the impact of structural inhomogeneity, further exaggerated by the large-current charging, can lead to severely uneven lithium plating and eventually cell failure. Therefore, a codesign strategy involving delicate controls of microstructure and electrochemical conditions could be a necessity for the next-generation battery with lithium metal anode.
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http://dx.doi.org/10.1021/acs.nanolett.1c01389DOI Listing
June 2021

Solvent polarity driven helicity inversion and circularly polarized luminescence in chiral aggregation induced emission fluorophores.

Chem Sci 2020 Aug 17;11(36):9989-9993. Epub 2020 Aug 17.

Advanced Materials and Liquid Crystal Institute, Chemical Physics Interdisciplinary Program, Kent State University Kent OH 44242 USA

Development of functional materials capable of exhibiting chirality tunable circularly polarized luminescence (CPL) is currently in high demand for potential technological applications. Herein we demonstrate the formation of both left- and right-handed fluorescent helical superstructures from each enantiomer of a chiral tetraphenylethylene derivative through judicious choice of the solution processing conditions. Interestingly, both the aggregation induced emission active enantiomers exhibit handedness inversion of their supramolecular helical assemblies just by varying the solution polarity without any change in their molecular chirality. The resulting helical supramolecular aggregates from each enantiomer are capable of emitting circularly polarized light, thus enabling both right- and left-handed CPL from a single chiral material. The left- and right-handed supramolecular helical aggregates in the dried films have been characterized using spectroscopy, scanning electron microscopy, and transmission electron microscopy techniques. These new chiral aggregation induced emission compounds could find applications in devices where CPL of opposite handedness is required from the same material and would facilitate our understanding of the formation of helical assemblies with switchable supramolecular chirality.
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http://dx.doi.org/10.1039/d0sc04179cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162095PMC
August 2020

The biological role of MutT in the pathogenesis of the zoonotic pathogen serotype 2.

Virulence 2021 12;12(1):1538-1549

MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Lab of Animal Bacteriology, Ministry of Agriculture, OIE Reference Lab for Swine Streptococcosis, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

() is an important rising pathogen that causes serious diseases in humans and pigs. Although some putative virulence factors of have been identified, its pathogenic mechanisms are largely unclear. Here, we identified a putative virulence-associated factor MutT, which is unique to serotype 2 (SS2) virulent strains. To investigate the biological roles of MutT in the SS2 virulent strain ZY05719, the knockout mutant (Δ) was generated and used to explore the phenotypic and virulent variations between the parental and Δ strains. We found that the mutation significantly inhibited cell growth ability, shortened the chain length, and displayed a high susceptibility to HO-induced oxidative stress. Moreover, this study revealed that MutT induced the adhesion and invasion of SS2 to host cells. Deletion of increased microbial clearance in host tissues of the infected mice. Sequence alignment results suggested that was encoded in a strain-specific manner, in which the detection was strongly linked to bacterial pathogenicity. In both zebrafish and mice infection models, the virulence of Δ was largely reduced compared with that of ZY05719. Overall, this study provides compelling evidence that MutT is indispensable for the virulence of SS2 and highlights the biological role of MutT in bacteria pathogenesis during infection.
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http://dx.doi.org/10.1080/21505594.2021.1936770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183525PMC
December 2021

Blood Tumor Mutational Burden as a Predictive Biomarker in Patients With Advanced Non-Small Cell Lung Cancer (NSCLC).

Front Oncol 2021 14;11:640761. Epub 2021 May 14.

Yunnan Cancer Hospital and The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Center, Kunming, China.

This study was designed to investigate the impact of blood tumor mutational burden (bTMB) on advanced NSCLC in Southwest China. The relationship between the tTMB estimated by next-generation sequencing (NGS) and clinical outcome was retrospectively analyzed in tissue specimens from 21 patients with advanced NSCLC. Furthermore, the relationship between the bTMB estimated by NGS and clinical outcome was retrospectively assessed in blood specimens from 70 patients with advanced NSCLC. Finally, 13 advanced NSCLC patients were used to evaluate the utility of bTMB assessed by NGS in differentiating patients who would benefit from immunotherapy. In the tTMB group, tTMB ≥ 10 mutations/Mb was related to inferior progression-free survival (PFS) (hazard ratio [HR], 0.30; 95% CI, 0.08-1.17; log-rank = 0.03) and overall survival (OS) (HR, 0.30; 95% CI, 0.08-1.16; log-rank = 0.03). In the bTMB group, bTMB ≥ 6 mutations/Mb was associated with inferior PFS (HR, 0.32; 95% CI, 0.14-1.35; log-rank < 0.01) and OS (HR, 0.31; 95% CI, 0.14-0.7; log-rank < 0.01). In the immunotherapy section, bTMB ≥ 6 mutations/Mb was related to superior PFS (HR, 0.32; 95% CI, 0.14-1.35; log-rank < 0.01) and objective response rates (ORRs) (bTMB < 6: 14.2%; 95% CI, 0.03-1.19; bTMB ≥ 6: 83.3%; 95% CI, 0.91-37.08; = 0.02). These findings suggest that bTMB is a validated predictive biomarker for determining the clinical outcome of advanced NSCLC patients and may serve as a feasible predictor of the clinical benefit of immunotherapies (anti-PD-1 antibody) in the advanced NSCLC population in Yunnan Province.
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http://dx.doi.org/10.3389/fonc.2021.640761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160368PMC
May 2021

Loading Gentamicin and Zn on TiO Nanotubes to Improve Anticoagulation, Endothelial Cell Growth, and Antibacterial Activities.

Stem Cells Int 2021 3;2021:9993247. Epub 2021 May 3.

Faculty of Mechanical and Material Engineering, Huaiyin Institute of Technology, Huai'an 223003, China.

Titanium and its alloys are widely used in blood-contacting implantable and interventional medical devices; however, their biocompatibility is still facing great challenges. In the present study, in order to improve the biocompatibility and antibacterial activities of titanium, TiO nanotubes were firstly in situ prepared on the titanium surface by anodization, followed by the introduction of polyacrylic acid (PAA) and gentamicin (GS) on the nanotube surface by layer-by-layer assembly, and finally, zinc ions were loaded on the surface to further improve the bioactivities. The nanotubes displayed excellent hydrophilicity and special nanotube-like structure, which can selectively promote the albumin adsorption, enhance the blood compatibility, and promote the growth of endothelial cells to some degree. After the introduction of PAA and GS, although the superhydrophilicity cannot be achieved, the results of platelet adhesion, cyclic guanosine monophosphate (cGMP) activity, hemolysis rate, and activated partial thromboplastin time (APTT) showed that the blood compatibility was improved, and the blood compatibility was further enhanced after zinc ion loading. On the other hand, the modified surface showed good cytocompatibility to endothelial cells. The introduction of PAA and zinc ions not only promoted the adhesion and proliferation of endothelial cells but also upregulated expression of vascular endothelial growth factor (VEGF) and nitric oxide (NO). The slow and continuous release of GS and Zn over 14 days can significantly improve the antibacterial properties. Therefore, the present study provides an effective method for the surface modification of titanium-based blood-contacting materials to simultaneously endow with good blood compatibility, endothelial growth behaviors, and antibacterial properties.
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http://dx.doi.org/10.1155/2021/9993247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112940PMC
May 2021
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