Publications by authors named "Qiulan Huang"

9 Publications

  • Page 1 of 1

PAL-mediated SA biosynthesis pathway contributes to nematode resistance in wheat.

Plant J 2021 May 11. Epub 2021 May 11.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.

The pathogen cereal cyst nematode (CCN) is deleterious to Triticeae crops and is a threat to the global crop yield. Accession no. 1 of Aegilops variabilis, a relative of Triticum aestivum (bread wheat), is highly resistant to CCN. Our previous study demonstrated that the expression of the phenylalanine ammonia lyase (PAL) gene AevPAL1 in Ae. variabilis is strongly induced by CCN. PAL, the first enzyme of phenylpropanoid metabolism, is involved in abiotic and biotic stress responses. However, its role in plant-CCN interaction remains unknown. In the present study, we proved that AevPAL1 helps to confer CCN resistance through affecting the synthesis of salicylic acid (SA) and downstream secondary metabolites. The silencing of AevPAL1 increased the incidence of CCN infection in roots and decreased the accumulation of SA and phenylalanine (Phe)-derived specialized metabolites. The exogenous pre-application of SA also improved CCN resistance. Additionally, the functions of PAL in phenylpropanoid metabolism correlated with tryptophan decarboxylase (TDC) functioning in tryptophan metabolism pathways. The silencing of either AevPAL1 or AevTDC1 exhibited a concomitant reduction in the expression of both genes and the contents of metabolites downstream of PAL and TDC. These results suggested that AevPAL1, possibly in coordination with AevTDC1, positively contributes to CCN resistance by altering the downstream secondary metabolites and SA content in Ae. variabilis. Moreover, AevPAL1 overexpression significantly enhanced CCN resistance in bread wheat and did not exhibit significant negative effects on yield-related traits, suggesting that AevPAL1 is valuable for the genetic improvement of CCN resistance in bread wheat.
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http://dx.doi.org/10.1111/tpj.15316DOI Listing
May 2021

Rewiring central carbon metabolism for tyrosol and salidroside production in Saccharomyces cerevisiae.

Biotechnol Bioeng 2020 08 15;117(8):2410-2419. Epub 2020 May 15.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Metabolic engineering of Saccharomyces cerevisiae for high-level production of aromatic chemicals has received increasing attention in recent years. Tyrosol production from glucose by S. cerevisiae is considered an environmentally sustainable and safe approach. However, the production of tyrosol and salidroside by engineered S. cerevisiae has been reported to be lower than 2 g/L to date. In this study, S. cerevisiae was engineered with a push-pull-restrain strategy to efficiently produce tyrosol and salidroside from glucose. The biosynthetic pathways of ethanol, phenylalanine, and tryptophan were restrained by disrupting PDC1, PHA2, and TRP3. Subsequently, tyrosol biosynthesis was enhanced with a metabolic pull strategy of introducing PcAAS and EcTyrA . Moreover, a metabolic push strategy was implemented with the heterologous expression of phosphoketolase (Xfpk), and then erythrose 4-phosphate was synthesized simultaneously by two pathways, the Xfpk-based pathway and the pentose phosphate pathway, in S. cerevisiae. Furthermore, the heterologous expression of Xfpk alone in S. cerevisiae efficiently improved tyrosol production compared with the coexpression of Xfpk and phosphotransacetylase. Finally, the tyrosol yield increased by approximately 135-folds, compared with that of parent strain. The total amount of tyrosol and salidroside with glucose fed-batch fermentation was over 10 g/L and reached levels suitable for large-scale production.
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http://dx.doi.org/10.1002/bit.27370DOI Listing
August 2020

Rational Engineering of Chorismate-Related Pathways in for Improving Tyrosol Production.

Front Bioeng Biotechnol 2019 3;7:152. Epub 2019 Jul 3.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Tyrosol is extensively used in the pharmaceutical industry as an important natural product from plants. In this study, an exogenous pathway involved in catalyzing tyrosine to tyrosol was introduced into . Furthermore, The pyruvate decarboxylase gene was deleted to redirect the flux distribution at the pyruvate node, and a bifunctional NAD-dependent fused chorismate mutase/prephenate dehydrogenase from (TyrA) and its' tyrosine inhibition resistant mutant (TyrA) were heterologously expression in to tuning up the chorismate metabolism effectively directed the metabolic flux toward tyrosol production. Finally, the tyrosol yield of the engineered strain GFT-4 was improved to 126.74 ± 6.70 mg/g DCW at 48 h, increased 440 times compared with that of the control strain GFT-0 (0.28 ± 0.01 mg/g DCW). The new synergetic engineering strategy developed in this study can be further applied to increase the production of high value-added aromatic compounds derived from aromatic amino acid or shikimate in .
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http://dx.doi.org/10.3389/fbioe.2019.00152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6616077PMC
July 2019

Alcohol consumption and diabetes risk in a Chinese population: a Mendelian randomization analysis.

Addiction 2019 03 25;114(3):436-449. Epub 2018 Nov 25.

Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Aim: To assess the causality between alcohol intake, diabetes risk and related traits.

Design: Mendelian randomization (MR) study. Subgroup analysis, standard instrumental variable analysis and local average treatment effect (LATE) methods were applied to assess linear and non-linear causality.

Setting: China.

Participants: A total of 4536 participants, including 721 diabetes cases.

Findings: Carriage of an ALDH2 rs671 A allele reduced alcohol consumption by 44.63% [95% confidence interval (CI) = -49.44%, -39.37%]. In males, additional carriage of an A allele was significantly connected to decreased diabetes risk for the overall population [odds ratio (OR) = 0.716, 95% CI = 0.567-0.904, P = 0.005] or moderate drinkers (OR = 0.564, 95% CI = 0.355-0.894, P = 0.015). In instrumental variable (IV) analysis, increasing alcohol consumption by 1.7-fold was associated with an incidence-rate ratio of 1.32 (95% CI = 1.06-1.67, P = 0.014) for diabetes risk, and elevated alcohol intake was causally connected to natural log-transformed fasting, 2-hour post-load plasma glucose (β = 0.036, 95% CI = 0.018-0.054; β = 0.072, 95% CI = 0.035-0.108) and insulin resistance [homeostatic model assessment for IR (HOMA-IR] (β = 0.104, 95% CI = 0.039-0.169), but was not associated with beta-cell function (HOMA-beta). In addition, the LATE method did not identify significant U-shaped causality between alcohol consumption and diabetes-related traits. In females, the effects of alcohol intake on all the outcomes were non-significant.

Conclusion: Among men in China, higher alcohol intake appears to be causally associated with increased diabetes risk and worsened related traits, even for moderate drinkers. This study found no significant U-shaped causality between alcohol consumption and diabetes-related traits.
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http://dx.doi.org/10.1111/add.14475DOI Listing
March 2019

The Gene From Regulate the Resistance Against Cereal Cyst Nematode by Altering the Downstream Secondary Metabolite Contents Rather Than Auxin Synthesis.

Front Plant Sci 2018 4;9:1297. Epub 2018 Sep 4.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.

Cereal cyst nematode (CCN, ) is a most important pathogen of wheat and causes tremendous yield loss annually over the world. Since the lack of resistance materials among wheat cultivars, identification and characterization of the resistance-related genes from the relatives of wheat is a necessary and efficient way. As a close relative of wheat with high resistance against CCN, is believed to be a valuable source for wheat breeding against this devastating disease. However so far, very few resistance-associated genes have been characterized from this species. In this study, we present that the genes from ( and ) were both induced by CCN juveniles at the early stage of resistance response (30 h post-inoculation), with more sensitive to CCN infection than . Silencing of led to compromised immunity to CCN with more CCN intrusion into roots; while overexpression in dramatically enhanced the resistance of plants by reducing the knots formed on roots. Metabolism analysis showed that the contents of secondary metabolites with activity of resistance to varied pathogens correlated with the expression level of in both and the transgenic tobacco plants. In addition, the content of IAA was not affected by either silencing or overexpressing of . Hence, our research provided a valuable target that mediates resistance to CCN and root knot nematode (RKN, ) without influencing the auxin biosynthesis.
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http://dx.doi.org/10.3389/fpls.2018.01297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6132075PMC
September 2018

Polarization-orthogonal filament array induced by birefringent crystals in air.

Opt Express 2018 Apr;26(7):8515-8521

We demonstrate the generation of filament array with orthogonal polarizations in air by using specifically designed wedge-type birefringent quartz plates. Experimental results show that the number of the generated filaments can be expressed as N = 2n wherenis the number of quartz plates inserted in the laser propagation path. By manipulating the optic axis of the quartz plates with respect to the polarization direction of the input laser pulse, the generated filaments can be separated into two parts with the polarization directions perpendicular with each other. The separation distance between the adjacent filaments is found to be linearly dependent on the focal length of external focusing lens. Our results provide a simple and efficient way to generate regular and reproductive femtosecond filament array in air.
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http://dx.doi.org/10.1364/OE.26.008515DOI Listing
April 2018

[Interleukin-9 promotes pancreatic cancer cell proliferation and migration via activation of STAT3 pathway].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2017 Sep;33(9):1228-1233

Department of Gastroenterology, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.

Objective To investigate the impact of interleukin-9 (IL-9) on proliferation, invasion and migration of pancreatic cancer cells and its mechanism. Methods PANC-1 cells were cultured in vitro and treated with IL-9 at different concentrations (5, 10, 20 ng/mL) for 24 hours. The level of IL-9R mRNA was analyzed by quantitative real-time PCR. CCK-8 assay was used to test the proliferation of the cells and flow cytometry to detect the cell apoptosis. Transwell assay was employed to determine the invasion and migration of PANC-1 cells. Western blotting was used to detect the STAT3 and p-STAT3 protein expression levels. After PANC-1 cells were treated with different concentrations of STAT3 pathway inhibitor AG490, followed by IL-9 treatment, the STAT3 and p-STAT3 protein expressions, as well as the proliferation of the cells were detected again. Results The level of IL-9R mRNA and the proliferation rate of PANC-1 cells were enhanced with the increase of IL-9 concentration, and the capacities of cell invasion and migration were promoted significantly. The relative protein expression of p-STAT3 increased greatly in PANC-1 cells after the treatment of IL-9, but STAT3 were not changed significantly compared with the ones without IL-9 treatment. The proliferation-promoting effect of IL-9 on AG490-pretreated PANC-1 cells was induced, and the p-STAT3 protein expression level was notably inhibited. Conclusion The activation of STAT3 pathway is strongly associated with the process that IL-9 mediates the promotion of proliferation, invasion and migration in pancreatic cancer cells.
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September 2017

[Association of liver enzyme and long-term weight growth in adults].

Zhonghua Gan Zang Bing Za Zhi 2015 Sep;23(9):694-6

Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

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http://dx.doi.org/10.3760/cma.j.issn.1007-3418.2015.09.012DOI Listing
September 2015