Publications by authors named "Qiuhua Li"

63 Publications

HS-SPME and GC/MS volatile component analysis of Yinghong No. 9 dark tea during the pile fermentation process.

Food Chem 2021 Apr 2;357:129654. Epub 2021 Apr 2.

Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, China.

Each type of tea has a unique volatile profile due to its variety, processing technologies and origin. Using HS-SPME and GC/MS, we analyzed the changes of volatile components in cultivar Yinghong No. 9 during pile-fermentation every 10 days. A total of 94 compounds showed significant differences during a total of 60 days mainly including alkanes, ketones, esters, terpenes, aromatics and heterocyclic compounds. Interestingly, 13 metabolites were progressively reduced during the first 20 days and remained unchanged in subsequent procedures, while 17 metabolites remained unchanged in the early stage and progressively increased during the last 20 days of pile fermentation, indicating that they are characteristic volatile compounds of raw material sun-dried green tea and dark tea, respectively. β-ionone, phenylethyl alcohol, and a-ionone could be the top three contributed aroma compounds in the final dark tea. Our study provides a theoretical basis for process and quality improvement of Yinghong No. 9.
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http://dx.doi.org/10.1016/j.foodchem.2021.129654DOI Listing
April 2021

Diet influence on mercury bioaccumulation as revealed by polyunsaturated fatty acids in zoobenthos from two contrasting environments: Chinese reservoirs and Swedish lakes.

Sci Total Environ 2021 Mar 29;782:146410. Epub 2021 Mar 29.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, PR China.

The bioaccumulation of mercury (Hg) and polyunsaturated fatty acids (PUFA) in zoobenthos varies across aquatic food webs. In this field study, contents of total Hg (THg), methylmercury (MeHg) and PUFA were investigated in zoobenthos of Chinese reservoirs and Swedish lakes, with contrasting environmental characteristics and algal diet sources, which can result in difference of Hg and PUFA in zoobenthos from these two habits. Using PUFA as dietary biomarkers of algae in zoobenthos, we evaluated effects of environmental factors and algal diet sources on the accumulation of THg, MeHg, and the highly required PUFA eicosapentaenoic (EPA, 20: 5n-3) and docosahexaenoic acid (DHA, 22:6n-3) in zoobenthos. Average THg and MeHg in zoobenthos were higher in Chinese reservoirs than Swedish lakes (p < 0.05). Average EPA content of zoobenthos was similar in these two habitats (p > 0.05), yet average DHA content of zoobenthos was higher in Chinese reservoirs than Swedish lakes (p < 0.05). Total Hg and MeHg contents of zoobenthos in Swedish lakes were predicted by environmental factors; e.g., negatively with pH and positively with dissolved organic carbon (DOC) concentrations, yet had no significant relationship with the algal dietary. In Chinese reservoirs, however, no environmental factor correlated well with THg contents in zoobenthos, and only DOC concentrations showed positive correlation with MeHg contents in zoobenthos. Besides, the algal dietary was also positively correlated with MeHg contents in zoobenthos. EPA and DHA contents of zoobenthos in Swedish lakes primarily associated with algal diet. By contrast, in Chinese reservoirs, EPA and DHA contents of zoobenthos were affected by both environmental factors and algal diet.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146410DOI Listing
March 2021

Mercury, microcystins and Omega-3 polyunsaturated fatty acids in farmed fish in eutrophic reservoir: Risk and benefit assessment.

Environ Pollut 2021 Feb 10;270:116047. Epub 2020 Nov 10.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, PR China.

Fish is an important source of nutritional omega-3 (n-3) polyunsaturated fatty acids, but it also readily accumulates toxic mercury (Hg) and microcystins (MC) in eutrophic aquatic systems. In China, farmed fish was widely consumed, and aquaculture has caused pervasive eutrophication of freshwater lakes, resulting in the increasing accumulation of MC in fish tissue. To assess the risk-benefit of consuming farmed fish, 205 fish samples of 10 primary species were collected from the eutrophic Wujiangdu (WJD) Reservoir, SW China. The contents of Hg, microcystin-RR (MC-RR), microcystin-LR (MC-LR), and polyunsaturated fatty acids (PUFA) in fish were analyzed. The results showed that THg and MeHg concentrations in all fish sampls were well below the safety limit (500 ng/g w.w) established by the Standardization Administration of China, with average values of 22.9 ± 22.8 and 6.0 ± 6.6 ng/g wet weight (w.w.), respectively. Average concentrations of MC-RR and MC-LR were 40 ± 80 and 50 ± 80 ng/g w.w., respectively. MC-RR and MC-LR concentrations in fish were significantly higher in silver carp and black carp than in perch and catfish (p < 0.05). In nutritional terms, average concentrations of n-3 PUFA and the eicosapentaenoic (EPA) + docosahexaenoic acids (DHA) of fish were 2.0 ± 2.5 and 1.4 ± 0.5 mg/g w.w., respectively. The risk-benefit assessment suggests that the n-3 PUFA benefits from consuming all farmed fish species in the WJD Reservoir outweigh the adverse effects of MeHg. However, except for perch, most fish species still pose a high MC-LR exposure risk that created a requirement for fish consumption advisories and monitoring. Consequently, more attention should be paid on the health risk of combined exposure to pollutants by aquatic product consumption.
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http://dx.doi.org/10.1016/j.envpol.2020.116047DOI Listing
February 2021

Preventative and Therapeutic Potential of Flavonoids in Peptic Ulcers.

Molecules 2020 Oct 11;25(20). Epub 2020 Oct 11.

Tea Research Institute, Guangdong Academy of Agricultural Sciences or Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, China.

Peptic ulcer disease is a common gastrointestinal tract disorder that affects up to 20% of the population of the world. Treatment of peptic ulcer remains challenging due to the limited effectiveness and severe side effects of the currently available drugs. Hence, natural compounds, owing to their medicinal, ecological, and other safe properties, are becoming popular potential candidates in preventing and treating peptic ulcers. Flavonoids, the most abundant polyphenols in plants, exhibit gastroprotective effects against peptic ulcer both in vivo and in vitro. In this review, we summarized the anti-ulcer functions and mechanisms, and also the bioavailability, efficacy, and safety, of flavonoid monomers in the gastrointestinal tract. Flavonoids exerted cytoprotective and rehabilitative effects by not only strengthening defense factors, such as mucus and prostaglandins, but also protecting against potentially harmful factors via their antioxidative, anti-inflammatory, and antibacterial activities. Although controlled clinical studies are limited at present, flavonoids have shown a promising preventable and therapeutic potential in peptic ulcers.
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http://dx.doi.org/10.3390/molecules25204626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7594042PMC
October 2020

Large yellow croaker peroxiredoxin IV protect cells against oxidative damage and apoptosis.

Mol Immunol 2020 11 21;127:150-156. Epub 2020 Sep 21.

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China. Electronic address:

Oxidative stress and inflammation lead to cell damage and are implicated in many disease states. High concentrations of hydrogen peroxide (HO) may mediate cells apoptosis by increasing intracellular reactive oxygen species (ROS) levels. In this study, we established a LYCK-PrxIV cell line (large yellow croaker head kidney cell line stably expressing peroxiredoxin IV). The level of nitric oxide (NO), superoxide anion and hydrogen peroxide (HO) in this LYCK-PrxIV cells were significantly lower than those in control cells of LYCK-pcDNA3.1 (LYCK cell line stably transfected by pcDNA3.1 vector). Additionally, when exposed to HO, cell apoptosis was significantly alleviated in LYCK-PrxIV than in control cells. Meanwhile, the ROS level and ATP content were maintained more stable in LYCK-PrxIV than in LYCK-pcDNA3.1. The over-expression of LcPrxIV in LYCK-PrxIV cells induced a declined mRNA expression of LcCXC, LcCC, LcIL-8 and LcTNF-α2, as well as an increase of LcIL-10 mRNA expression, when compared to LYCK-pcDNA3.1. On the other hand, the expression of chemokine LcCXC, LcCC and LcTNF-a2 increased in LYCK-pcDNA3.1 after HO stimulation, while that of LcIL-8 and LcIL-10 decreased. The regualtion of gene expression in LYCK-PrxIV cells was almost the same as that in LYCK-pcDNA3.1, but the change fold was much more moderate. These results suggest that LcPrxIV may be an indispensable ROS scavenger protecting LYCK cells against oxidative damage as well as the subsequent apoptosis and inflammatory response, which provides a clue that LcPrxIV may be an assist in fish immune response.
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http://dx.doi.org/10.1016/j.molimm.2020.08.019DOI Listing
November 2020

Assessment of heavy metals contamination and water quality characterization in the Nanming River, Guizhou Province.

Environ Geochem Health 2021 Mar 10;43(3):1273-1286. Epub 2020 Sep 10.

Key Laboratory for Information and Computing Science of Guizhou Province, Guizhou Normal University, Guiyang, 550001, People's Republic of China.

The analysis to assess the water quality and potential ecological risks in sediments was carried out by means of the distribution characteristics of nutrient properties and heavy metals in water, and heavy metals in sediments from the Nanming River. The results from nutrient properties demonstrated that the majority of TN and TP exceeded the permissible limit and concentrated within the study area. The concentrations of heavy metal in water were lower than the permissible limits but may pose potential threat to aquatic ecosystems. Based on the potential ecological risk results of heavy metals in sediments, Cd posed risk to ecological environment, and the serious contaminations mainly existed in the center of Guiyang City. The multivariate statistical analyses were used to support the idea that the Upstream Area and Midstream Area were significantly dominated by NH, TP, TN and COD in water. Furthermore, landscape characteristics and hydrology condition better explained the certain trend of water quality. Finally, identifying relationship between nutrient properties and heavy metals that are key ecological components of ecosystem can potentially aid the advances for restoration of geochemical transformations and give rise to river restoration efforts.
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http://dx.doi.org/10.1007/s10653-020-00710-3DOI Listing
March 2021

Total mercury and methylmercury in the soil and vegetation of a riparian zone along a mercury-impacted reservoir.

Sci Total Environ 2020 Oct 1;738:139794. Epub 2020 Jun 1.

Faculty of Foreign Studies, Beijing Language and Culture University, Beijing 100083, China.

The Baihua Reservoir (Guizhou Province, Southwest China) has a history of mercury contamination associated with past acetic acid production activities at the Guizhou Organic Chemical Plant (GOCP). Soil and plant samples collected from riparian zones were analyzed for total Hg (THg) and methylmercury (MeHg) using cold vapor atomic fluorescence spectroscopy. The concentrations of THg and MeHg in soil samples were in the range of 109-371 ng g and 0.32-1.80 ng g, respectively. Soils in the riparian zones close to the pollution source (the GOCP) presented higher Hg contamination, with relatively light Hg contamination in remote areas. This suggests a decreasing trend of THg concentrations along the riparian zones, with higher concentrations closer to the pollution source. Significant correlations were found between MeHg and soil organic matter (n = 24, p = 0.01). THg concentrations varied 11.3-161 ng g in aboveground areas and 11.3-193 ng g in underground areas. MeHg concentrations ranged from 0.23 to 1.06 ng g in aboveground areas to 0.13-1.51 ng g in the below ground areas. The vegetation studied showed different concentrations of THg and MeHg and can be considered to be impacted by Hg contamination. Different concentrations of total and methyl mercury were found among the different plant species. The high Hg concentrations in soils and vegetation suggests that the ability of Hg to bioaccumulate in riparian plants is affected by plant physiological characteristics and soil mercury concentrations. Although the bioaccumulation factors (BCFs) of the studied plants were low, their transfer factors (TFs) were >1. Our findings suggest that vegetation exhibiting TFs for THg >1 have the potential for phytoextraction in Hg-impacted riparian zones.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139794DOI Listing
October 2020

Mercury isotope signatures of a pre-calciner cement plant in Southwest China.

J Hazard Mater 2021 01 4;401:123384. Epub 2020 Jul 4.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, China. Electronic address:

Characterizing the composition of mercury (Hg) isotopes in the atmospheric emissions of cement plants is critical to understand the global circulation of Hg because large quantities of Hg are released from this source annually. A pre-calciner cement plant in Guizhou Province in Southwest China was selected to investigate the mass dependent fractionation (MDF) and mass independent fractionation (MIF) of Hg in the entire production process and the speciated Hg isotope composition in stack gas. Significant MDF and insignificant MIF were observed in this cement plant. Different raw/correction materials have δHg signals ranging from -1.68 to -2.19‰. Raw meal is featured with lighter Hg (δHg = -2.83 ± 0.18‰) as results of Hg circulation and accumulation during the clinker production. Cement products possess negative δHg values (-1.98 ± 0.02‰) due to the input of light δHg isotopes through additives/retarder limestone, and fly ash and gypsum from coal-fired power plant (CFPPs). Speciated Hg isotopes in the stack gas of the kiln tail and kiln head show no significant differences, and δHg and ΔHg in the discharged flue gas averaged at -2.03 ± 0. 31‰ and -0.03 ± 0.07‰, respectively, which has negative δHg characteristics with other anthropogenic sources.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123384DOI Listing
January 2021

Regulation of Catechins in Uric Acid Metabolism Disorder Related Human Diseases.

Mini Rev Med Chem 2020 ;20(18):1857-1866

College of Horticulture, South China Agricultural University, Guangzhou 510000, China.

Uric acid is the end product of purine metabolism in humans. High uric acid levels form sodium urate crystals that trigger biological processes, which lead to the development of several diseases, including diabetes, hyperuricemia, gout, inflammatory disease, kidney disease, cardiovascular disease and hypertension. Catechins have been suggested to be beneficial for the regulation of uric acid metabolic disorders due to their powerful antioxidant and anti-inflammatory properties. To identify an effective and safe natural substance that can decrease levels of serum uric acid to improve uric acid metabolism disorders. A search was performed on PubMed, Web of Science and Google Scholar to identify comprehensive studies that presented summarized data on the use of catechins in lowering uric acid levels in diseases. This review details the role of catechins in inhibiting the activity of xanthine oxidase to decrease uric acid overproduction in the liver and in regulating expressions of uric acid transporters, URAT1, OAT1, OAT3, ABCG2 and GLUT9, to balance levels of uric acid secretion and reabsorption through the kidney and intestine. Additionally, Catechins were also found to prevent monosodium urate-induced inflammatory reactions. In vivo, catechins can be used to decrease high uric acid levels that result from hyperuricemia and related diseases. Catechins can be used to maintain the balance of uric acid metabolism.
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http://dx.doi.org/10.2174/1389557520666200719015919DOI Listing
January 2020

Antiobesity and anti-inflammation effects of Hakka stir-fried tea of different storage years on high-fat diet-induced obese mice model via activating the AMPK/ACC/CPT1 pathway.

Food Nutr Res 2020 8;64. Epub 2020 Jun 8.

Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, China.

Background: As a typical representative of metabolic syndrome, obesity is also one of the extremely dangerous factors of cardiovascular diseases. Thus, the prevention and treatment of obesity has gradually become a global campaign. There have been many reports that green tea is effective in preventing obesity, but as a kind of green tea with regional characteristics, there have been no reports that Hakka stir-fried tea (HT) of different storage years has a weight loss effect.

Aims: The aim was to investigate the effect of HT in diet-induced obese mice.

Methods: The mice were divided into five groups as follows: the control group received normal diet; the obese model group received high-fat diet; and HT2003, HT2008, and HT2015 groups, after the induction of obesity via a high-fat diet, received HT of different storage years treatment for 6 weeks, respectively.

Results: It was observed that HT decreased the levels of serum and liver triglyceride; the ratio of liver to body weight; accumulation of epididymal, perirenal, and mesenteric fat; the degree of hepatic steatosis; and adipocyte hypertrophy, with the concomitant reduction of body weight. Moreover, HT decreased the expression levels of proinflammatory cytokines tumor necrosis factor α (TNF α), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and reduced fatty acid synthase (FAS) activity in liver tissue of obese mice. In addition, HT treatment also increased the phosphorylation of AMP-activated protein kinase (AMPK) and its direct downstream proteins, acetyl coenzyme A carboxylase (ACC), and carnitine palmitoyltransferase I (CPT-1), which participate in FAS pathway.

Conclusions: These findings demonstrate that HT treatment has a potential protection on high-fat diet-induced obesity mice via activating the AMPK/ACC/CPT1 pathway, and to a certain extent, it has nothing to do with the storage time of three kinds of HT.
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http://dx.doi.org/10.29219/fnr.v64.1681DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286352PMC
June 2020

Erratum to "Molecular cloning and functional characterization of c-Jun in large yellow croaker (Larimichthys crocea)" [Fish Shellfish Immunol. 98 (2020) 981-987].

Fish Shellfish Immunol 2020 May 14;100:498. Epub 2020 Mar 14.

Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China. Electronic address:

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http://dx.doi.org/10.1016/j.fsi.2020.03.019DOI Listing
May 2020

Response of Bacillus vallismortis sp. EPS to exogenous sulfur stress/ induction and its adsorption performance on Cu(II).

Chemosphere 2020 Jul 2;251:126343. Epub 2020 Mar 2.

School of Environmental Science and Engineering of Guangdong University of Technology, Guangzhou, 510006, China.

The chemical composition of EPS (Extracellular Polymeric Substances) produced by Bacillus vallismortis sp. and its adsorption performance on typical heavy metal were studied under NaS stress/induction at different concentrations. Its structure was characterized by three-dimensional fluorescence spectrogram (3D-EEM), infrared spectrum (FTIR) and X-ray photoelectron spectroscopy (XPS). The results showed that, when the NaS stress/induction intensity was 20 mg/L, the protein concentration was nearly doubled compared with Control-EPS (EPS produced by Bacillus vallismortis sp. without exogenous sulfur stress); furthermore, the 3D-EEM results also demonstrated that there was an increase in the protein content, with the -SH content reaching 154.36 μmol/L, which was 48.2% higher than before stress (104.15 μmol/L). Under this condition, S-EPS (EPS produced by Bacillus vallismortis sp. stressed by exogenous sulfur) exhibited the best adsorption effect on Cu(II), with the theoretical maximum adsorption capacity reaching 1428.57 mg/g EPS. FTIR and XPS analyses revealed that the -SH, CO, N-H played a major role in the adsorption of Cu(II); among those, -SH played a key role. Moreover, the adsorption capacity of Cu(II) by S-EPS was correlated with the content of sulfhydryl protein; indeed, the exogenous sulfur stress/induction can effectively regulate the chemical composition of EPS and improve its adsorption performance, which can be crucial in the prevention and control of heavy metal pollution.
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http://dx.doi.org/10.1016/j.chemosphere.2020.126343DOI Listing
July 2020

Theaflavin TF3 Relieves Hepatocyte Lipid Deposition through Activating an AMPK Signaling Pathway by targeting Plasma Kallikrein.

J Agric Food Chem 2020 Mar 25;68(9):2673-2683. Epub 2020 Feb 25.

Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, China.

Nonalcoholic fatty liver disease (NAFLD) is rapidly becoming the leading cause of chronic liver diseases throughout the world. The deficit of pharmacotherapy for NAFLD calls for an urgent need for a new drug discovery and lifestyle management. Black tea is the most popular and functional drink consumed worldwide. Its main bioactive constituent theaflavin helps to prevent obesity-a major risk factor for NAFLD. To find new targets for the development of effective and safe therapeutic drugs from natural plants for NAFLD, we found a theaflavin monomer theaflavin-3,3'-digallate (TF3), which significantly reduced lipid droplet accumulation in hepatocytes, and directly bound and inhibited the activation of plasma kallikrein (PK), which was further proved to stimulate adenosine monophosphate activated protein kinase (AMPK) and its downstream targets. Taken together, we proposed that the TF3-PK-AMPK regulatory axis is a novel mechanism of lipid deposition mitigation, and PK could be a new target for NAFLD treatment.
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http://dx.doi.org/10.1021/acs.jafc.0c00148DOI Listing
March 2020

Green tea and black tea inhibit proliferation and migration of HepG2 cells via the PI3K/Akt and MMPs signalling pathway.

Biomed Pharmacother 2020 May 28;125:109893. Epub 2020 Jan 28.

Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, 510640, PR China. Electronic address:

Background And Aims: Black tea and green tea were produced via different processing techniques from the same tea leave variety. Then, biochemical components of the two water extracts were analysed to study cell apoptosis, migration and invasion of HepG2 cells induced by black tea and green tea.

Method: The monomer components of the black tea and green tea extracts were analysed by colorimetry and HPLC, with MTT assay and colony formation assays used to assess cell proliferation and viability. The effects of black tea and green tea on apoptosis of HepG2 cells were verified by flow cytometry, with wound healing and Transwell experiments used to detect cell invasion and metastasis. The expression of PI3K/Akt signalling and apoptosis-related proteins as well as epithelial-mesenchymal transition (EMT) regulatory factor in HepG2 cells were determined by western blotting after black tea and green tea treatment.

Results And Conclusions: Black tea and green tea extracts demonstrated different degrees of inhibition of cell migration and invasion, with green tea inducing more HepG2 cell apoptosis. In addition, green tea and black tea extracts inhibited the growth of HepG2 cells and induced apoptosis via PI3K/Akt, and inhibited cell migration and invasion through the MMPs signalling pathway. This study revealed the effects of fermented (black tea) and non-fermented tea (green tea) on liver cancer cells, providing a basis for the investigation of tea extracts for their anti-tumour potential.
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http://dx.doi.org/10.1016/j.biopha.2020.109893DOI Listing
May 2020

Identification and bioactivity of a granulocyte colony-stimulating factor a homologue from large yellow croaker (Larimichthys crocea).

Fish Shellfish Immunol 2020 Mar 7;98:167-175. Epub 2020 Jan 7.

Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519000, China. Electronic address:

Granulocyte colony-stimulating factor (GCSF) is a growth factor that drives the proliferation and differentiation of granulocytes and monocytes/macrophages. Currently, two copies of GCSF, named GCSFa and GCSFb, have been identified in teleost fish, but data on the functions and signal pathways of these fish GCSFs are still limited. In the present study, a GCSFa homologue (LcGCSFa) was identified from large yellow croaker (Larimichthys crocea). The open reading frame (ORF) of LcGCSFa is 636 bp long and encodes a protein of 211 amino acids (aa), with a 19-aa signal peptide and a typical IL-6 domain, conserved in fish GCSF sequences. The phylogenetic analysis showed that LcGCSFa clustered with other fish GCSFa homologues. LcGCSFa was constitutively expressed in all tissues tested and significantly up-regulated in head kidney and spleen by Vibrio alginolyticus or poly(I:C). LcGCSFa transcripts were also detected in primary head kidney leucocytes (PKL), primary head kidney macrophages (PKM), and primary head kidney granulocytes (PKG), and significantly up-regulated in PKL and PKG by LPS or poly(I:C). These data indicated that LcGCSFa may be involved in the immune responses induced by bacterium and virus. The recombinant LcGCSFa protein (rLcGCSFa) produced in Pichia pastoris promoted the proliferation of PKL both in vivo and in vitro. Furthermore, rLcGCSFa significantly increased both transcription and phosphorylation levels of the signal transducers and activators of transcription (STAT) proteins (LcSTAT3 and LcSTAT5) in PKL, which are required for the GCSF-dependent proliferation. These results showed that LcGCSFa may promote the proliferation of PKL via the activation of LcSTAT3 and LcSTAT5, suggesting a conserved role across vertebrate GCSFs.
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http://dx.doi.org/10.1016/j.fsi.2020.01.003DOI Listing
March 2020

Profiling of differentially expressed circular RNAs in peripheral blood mononuclear cells from Alzheimer's disease patients.

Metab Brain Dis 2020 01 13;35(1):201-213. Epub 2019 Dec 13.

Department of Neurology, The Affiliated Hospital of Jining Medical University, Jining, 272000, China.

Expression of circular RNA (circRNA), a class of noncoding RNAs that regulates gene expression, is altered in Alzheimer's disease. This study profiled differentially expressed circRNAs in peripheral blood mononuclear cells (PBMCs) from five patients with Alzheimer's disease compared to healthy controls using circRNA microarrays. We identified a total of 4060 differentially expressed circRNAs (1990 upregulated and 2070 downregulated) in Alzheimer's disease patients. Among these circRNAs, 10 randomly selected circRNAs were verified using qRT-PCR. The top 10 upregulated and downregulated circRNAs were used to predict their target miRNAs. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that these differentially expressed circRNAs were strongly associated with inflammation, metabolism, and immune responses, which are all risk factors for Alzheimer's disease. The circRNA-miRNA-mRNA network was most involved in the MAPK, mTOR, AMPK, and WNT signaling pathways in Alzheimer's disease. In conclusion, the current study demonstrated the importance of circRNAs in Alzheimer's disease development. Future studies will evaluate some of these circRNAs as biomarkers for early disease detection and to develop therapeutic strategies to clinically control Alzheimer's disease progression.
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http://dx.doi.org/10.1007/s11011-019-00497-yDOI Listing
January 2020

Effect of aquaculture on mercury and polyunsaturated fatty acids in fishes from reservoirs in Southwest China.

Environ Pollut 2020 Feb 1;257:113543. Epub 2019 Nov 1.

Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province Guizhou Normal University, Guiyang, 550000, PR China.

Aquaculture can affect the polyunsaturated fatty acids (PUFA) and mercury (Hg) in fish by altering their diet. Here, planktivorous (silver carp and bighead carp), omnivorous and carnivorous fish with different dietary strategies were selected from two reservoirs, one with on-going aquaculture (WJD) and another without aquaculture (HF) in Southwest China. We compared the total mercury (THg), methylmercury (MeHg) contents and PUFA profiles of fish and their potential diets in these two reservoirs. THg and MeHg contents in omnivorous and carnivorous fish were lower from the WJD Reservoir, which is related to the lower THg and MeHg contents in the artificial fish food. THg and MeHg contents in silver carp from the WJD Reservoir were lower than those from the HF Reservoir, while they were similar in bighead carps from the two reservoirs. The Hg variation in planktivorous fish were inconsistent with that in plankton. THg contents in phyto- and zooplankton from the HF Reservoir were higher than those from the WJD Reservoir, yet their MeHg contents were similar. Artificial fish food which contained higher total PUFA eicosapentaenoic (EPA; 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3), significantly increased the total PUFA and EPA + DHA contents in carnivorous fish, but had less effect on that in omnivorous fish from the WJD Reservoir. Eutrophication caused by aquaculture reduced total PUFA and EPA + DHA contents of plankton in WJD, yet did not reduce those in planktivorous fish. The impacts of aquaculture on Hg and PUFA accumulated in fish were varied among different fish species, and the mechanism needs further exploration.
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http://dx.doi.org/10.1016/j.envpol.2019.113543DOI Listing
February 2020

Molecular cloning.

Fish Shellfish Immunol 2020 Mar 31;98:981-987. Epub 2019 Oct 31.

Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China. Electronic address:

Transcription factor c-Jun is a member of AP-1 transcription complex that can be induced by various pathogens and plays a broad regulatory role in vertebrate immune response. In this study, the complete c-Jun cDNA of large yellow croaker Larimichthys crocea (Lcc-Jun) was cloned, whose open reading frame (ORF) is 984 bp long and encodes a protein of 327 amino acids (aa). The deduced Lcc-Jun protein contains three highly conserved domains, a transactivation domain (TAD, Met-His), a DNA binding domain (DBD, Lys-Arg), and a Leucine zipper domain (LZD, Leu-Leu), as found in other specie c-Jun. Lcc-Jun was constitutively expressed in all examined tissues, with the higher levels in blood, heart, and head kidney. Its transcripts were not only induced in spleen and head kidney by poly (I: C) or LPS, but also up-regulated in primary head kidney leukocytes (PKL), macrophages (PKM), and granulocytes (PKG), suggesting that Lcc-Jun may be involved in immune responses induced by poly (I: C), a viral mimic, and LPS, a Gram-negative bacterial component. Overexpression of Lcc-Jun in PKL increased the expression of cytokines and transcription factors involved in T helper 1 (Th1: TNF-α, IFN-γ, and T-bet) and Th2 (IL-4/13 A/B, IL-6, and GATA3) cell development and differentiation, suggesting that Lcc-Jun may play a role in regulation of Th1/Th2 cell response. These results therefore led us to suggest that the c-Jun-mediated signaling pathways may have an important immune-modulatory function in teleost fish.
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http://dx.doi.org/10.1016/j.fsi.2019.10.064DOI Listing
March 2020

A combination of Citrus reticulata peel and black tea inhibits migration and invasion of liver cancer via PI3K/AKT and MMPs signaling pathway.

Mol Biol Rep 2020 Jan 30;47(1):507-519. Epub 2019 Oct 30.

Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou, 510640, People's Republic of China.

Liver cancer, one of the most common malignancies, is the second leading cause of cancer death in the world. The citrus reticulate peel and black tea have been studied for their beneficial health effects. In spite of the many studies have been reported, the underlying molecular mechanisms underlying its health benefits are still not fully understood. In present study, we developed a unique citrus reticulate peel black tea (CRPBT) by combined citrus reticulate peel and black tea and assessed its active ingredients, anti-oxidant and anti-liver cancer effects in vitro. The results suggested that CRPBT exhibited antioxidant capacity and effectively inhibited proliferation and migration of liver cancer cells in a dose- and time- dependent manner. Mechanistically, CRPBT significantly down-regulated phosphorylation of PI3K and AKT, and up-regulated the ratio of Bax/Bcl-2, and suppressed the expression of MMP2/9, N-cadherin and Vimetin proteins in liver cancer cells. Taken together, CRPBT has good effect on inhibiting migration, invasion, proliferation, and inducing apoptosis in liver cancer cells.
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http://dx.doi.org/10.1007/s11033-019-05157-zDOI Listing
January 2020

Composition change and adsorption performance of EPS from Bacillus vallismortis sp. induced by NaS.

Ecotoxicol Environ Saf 2019 Dec 21;185:109679. Epub 2019 Sep 21.

School of Environmental Science and Engineering of Guangdong University of Technology, Guangzhou, Guangdong, 510006, China.

Sodium sulfide (NaS) was used as an inducer to regulate the components of Bacillus vallismortis sp. EPS (Extracellular Polymeric Substances). The main objective of this study was to improve the content of sulfhydryl protein and the adsorption property of EPS to Zn (Ⅱ) that as an typical heavy metal. The results showed that the maximum EPS production of 105.58 mg/g VSS coupling with doubled increase in protein in which the contant of -SH increased by 48.2% from 104.15 to 154.36 μmol/L were recorded in the presence of 20 mg/L NaS. Under this condition, the adsorption capacity of S-EPS (EPS with added exogenous NaS) for Zn (Ⅱ) was highest. The kinetics of the adsorption process of Zn (Ⅱ) by the S-EPS can be well fitted by the Langmuir isotherm model and the theoretical maximum adsorption amount of 979.09 mg/g EPS could be obtained. The results of 3D-EEM and FTIR analyses, illustrated that -SH, CO, and N-H/C-N played major roles in the removal of Zn (Ⅱ) by S-EPS. The results obtained in this study demonstrated that the addition of sulfur source could increase the content of sulfhydryl protein, and effectively regulate the content of chemical composition, expecially for the sulfhydryl of EPS, and thereby greatly improving the removal efficiency of heavy metals, which showed a great application prospect in the prevention and control of heavy metal pollution.
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http://dx.doi.org/10.1016/j.ecoenv.2019.109679DOI Listing
December 2019

Effects of typical algae species (Aphanizomenon flosaquae and Microcystis aeruginosa) on photoreduction of Hg in water body.

J Environ Sci (China) 2019 Nov 26;85:9-16. Epub 2019 Feb 26.

School of Chemistry and Material, Guizhou Normal University, Guiyang, China.

Photoreduction characteristics of divalent inorganic mercury (Hg) in the presence of specific algae species are still not well known. Laboratory experiments were conducted in the present study to identify the effects of different concentrations of living/dead algae species, including Aphanizomenon flosaquae (AF) and Microcystis aeruginosa (MA), on the photoreduction rate of Hg under various light conditions. The experimental results showed that percentage reduction of Hg was significantly influenced by radiation wavelengths, and dramatically decreased with the presence of algae. The highest percentage reduction of Hg was induced by UV-A, followed by UV-B, visible light and dark for both living and dead AF, and the order was dark > UV-A > UV-B > visible light for both living and dead MA. There were two aspects, i.e., energy and attenuation rate of light radiation and excrementitious generated from algae metabolisms, were involved in the processes of Hg photoreduction with the presence of algae under different light conditions. The percentage reduction of Hg decreased from 15% to 11% when living and dead AF concentrations increased by 10 times (from 10 to 10 cells/mL), and decreased from11% to ~9% in the case of living and dead MA increased. Algae can adsorb Hg and decrease the concentration of free Hg, thus inhibiting Hg photoreduction, especially under the conditions with high concentrations of algae. No significant differences were found in percentage reduction of Hg between living and dead treatments of algae species. The results are of great importance for understanding the role of algae in Hg photoreduction.
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http://dx.doi.org/10.1016/j.jes.2019.02.012DOI Listing
November 2019

Glial Plasticity in the Trigeminal Root Entry Zone of a Rat Trigeminal Neuralgia Animal Model.

Neurochem Res 2019 Aug 17;44(8):1893-1902. Epub 2019 Jun 17.

Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, China.

The trigeminal root entry zone (TREZ) is the transitional zone of central and peripheral tissue compartments in the trigeminal root. Microvascular compression on the TREZ is the main etiology of most idiopathic trigeminal neuralgia (TN) patients. However, the pathogenesis of TN is still uncertain. To investigate the glial plasticity changes in oligodendrocytes, Schwann cells, astrocytes and microglia/macrophages in the TREZ in TN, immunohistochemical staining and Western blot methods were performed in rats with TN induced by compression injury. The results showed that mechanical compression injury in the trigeminal nerve of the TN rats induced glial plasticity in the TREZ, which dynamically changed the glial interface of the CNS-PNS transitional zone. Additionally, glial fibrillary acidic protein (GFAP)-immunoreactive astrocyte processes significantly proliferated and extended distally from the central region to the peripheral side of the TREZ after nerve compression injury in the TN group. Moreover, the expression of p75 in Schwann cells was upregulated on the peripheral side of the TREZ, and activated Iba-1-immunoreactive microglia/macrophages were observed on both sides of the TREZ. A significantly higher number of Schwann cells, astrocytes and microglia/macrophages were found in the TN group than in the sham operation group (p < 0.05). In conclusion, mechanical compression injury in the TN rats activated various glial cells, including oligodendrocytes, astrocytes, Schwann cells and microglia/macrophages, in the CNS-PNS transitional zone of TREZ. Changes in glial cell plasticity in the TREZ after compression injury might be involved in TN pathogenesis.
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http://dx.doi.org/10.1007/s11064-019-02824-2DOI Listing
August 2019

Effect of yellowing time on bioactive compounds in yellow tea and their antiproliferative capacity in HepG2 cells.

Food Sci Nutr 2019 May 18;7(5):1838-1847. Epub 2019 Apr 18.

Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization Tea Research Institute Guangzhou China.

Several studies have shown potent antineoplastic effects of tea, which can induce apoptosis and inhibit proliferation of cancer cells. Yellow tea is one of the six major types of tea, and yellowing time, a key factor in its processing, is known to improve its quality and bioactivity. However, the effects of yellowing on the composition of the bioactive substances of tea are poorly understood. We analyzed the biochemical composition and the antioxidant and anticancer activities of the extracts of yellow tea (EYTs) subjected to different yellowing durations. Prolonged yellowing increased the content of water extracts, amino acids, soluble sugars, theaflavins, and nonesterified catechins (0.05,  < 0.01) and decreased that of polyphenols, flavonols, thearubigins, caffeine, GA, and esterified catechins (0.05,  < 0.01). In addition, yellowing also slightly increased the antioxidant capacity of the EYTs, but did not significantly affect their ability to inhibit the proliferation of the hepatocarcinoma HepG2 cells. Mechanistically, the EYTs significantly downregulated the phosphorylation of PI3K and AKT and upregulated the Bax/Bcl-2 ratio in the HepG2 cells. Taken together, the yellowing time influences the bioactive components of yellow tea, and the resulting yellow tea may have more potent antioxidant and anticancer effects.
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http://dx.doi.org/10.1002/fsn3.1036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526664PMC
May 2019

Development of monoclonal antibody against IgM of large yellow croaker (Larimichthys crocea) and characterization of IgM B cells.

Fish Shellfish Immunol 2019 Aug 20;91:216-222. Epub 2019 May 20.

Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, China; College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China. Electronic address:

In the present study, a monoclonal antibody (mAb) against large yellow croaker IgM was produced by immunizing mice with purified large yellow croaker serum IgM. Western blotting showed that this mAb could specifically react with the heavy chain of large yellow croaker serum IgM. Indirect immunofluorescence assay (IFA) analysis suggested that the resulting mouse anti-IgM mAb could recognize membrane-bound IgM (mIgM) molecules of large yellow croaker. This mouse anti-IgM mAb also can be used for sorting of large yellow croaker IgM B cells through the magnetic-activated cell sorting (MACS) method, which was further confirmed by RT-PCR analysis of specific marker genes for B cells. Flow cytometry analysis showed that the percentages of IgM B cells in head kidney, spleen and peripheral blood lymphocytes were 29.00 ± 1.58%, 33.00 ± 1.64%, and 16.50 ± 2.39%, respectively. Additionally, the phagocytosis rates of IgM B cells for 0.5 μm beads in head kidney, spleen and peripheral blood were calculated to be 7.56 ± 0.58%, 4.053 ± 0.62% and 23.17 ± 2.26%, respectively, while only 2.36 ± 0.23%, 1.16 ± 0.44% and 6.41 ± 0.45 of IgM B cells in these three tissues ingested 1 μm beads. Taken together, our data demonstrated that the mouse anti-IgM mAb produced in this study could be used as a tool to characterize IgM B cells and to study functions of IgM in large yellow croaker.
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http://dx.doi.org/10.1016/j.fsi.2019.05.035DOI Listing
August 2019

Research Progress of Mercury Bioaccumulation in the Aquatic Food Chain, China: A Review.

Bull Environ Contam Toxicol 2019 May 17;102(5):612-620. Epub 2019 May 17.

State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, 550081, China.

Research on mercury (Hg) in aquatic ecosystems in China has focused mainly on fish, with little research on the base of the food chain and Hg bioaccumulation mechanisms. This paper summarizes research progress pertaining to the characteristics, current status, and trends of Hg accumulation in the aquatic food chain in China, analyzes the effects of human activities on the transmission and accumulation of Hg in aquatic food chains, and assesses their risks to human and ecosystem health. A comparison of fish samples in China between 2000 and 2018 indicates that their total Hg content remains at relatively safe levels. However, because current information is generally insufficient to confirm how anthropogenic activities affect transformation and bioaccumulation in the aqueous environment, Hg isotope studies should be a focus of research on aquatic food webs. Additionally, more attention should be paid to Hg transport and bioaccumulation in the basic food chain by focusing on multi-contaminant joint exposure studies and establishing Hg bio-transport models.
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http://dx.doi.org/10.1007/s00128-019-02629-7DOI Listing
May 2019

Highly Cation Permselective Metal-Organic Framework Membranes with Leaf-Like Morphology.

ChemSusChem 2019 Jun 27;12(12):2593-2597. Epub 2019 May 27.

CAS Key Laboratory of Soft Matter Chemistry, iCHEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Chemistry, School of Chemistry and Materials University of Science and Technology of China, Hefei, 230026, China.

Highly cation permselective metal-organic framework (MOF) membranes are desirable for the extraction of valuable metal cations. However, fabrication of defect-free and stable permselective MOF membranes is technically challenging, owing to their arduous self-assembly and poor water resistance, respectively. A simple and readily scalable method has been developed for the controlled in situ smart growth of UiO-66-NH into leaf-like nanostructures with tunable density of the leaves and the surface layer thickness. The self-assembly approach reproducibly fabricates seamless, ultrathin (<500 nm) UiO-66-NH membranes at the surface of anodic aluminum oxide. The membranes contain nanosized interstices among the MOF leaves, which enable maximum admission of ions within the membrane, and angstrom-sized inherent pores in every single UiO-66-NH crystal, which efficiently regulate the cation permselectivity. Consequently, the highest ever reported cation separations (Na /Mg >200 and Li /Mg >60) and excellent membrane stability during five sequential electrodialysis cycles are achieved. These characteristics position the fabricated MOF membranes as potential candidates for efficient extraction of pure lithium and sodium ions from salt lakes and seawater, respectively.
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http://dx.doi.org/10.1002/cssc.201900706DOI Listing
June 2019

Identification and bioactivity of a granulocyte colony-stimulating factor b homologue from large yellow croaker (Larimichthys crocea).

Fish Shellfish Immunol 2019 Jul 19;90:20-29. Epub 2019 Apr 19.

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address:

Granulocyte colony-stimulating factor (GCSF) is a pleiotropic cytokine that plays a key role in regulation of hematopoiesis, innate and adaptive immune responses in mammals. However, bioactivity of GCSF in teleost fish remains largely unknown. In this study, a GCSFb homologue from large yellow croaker (Larimichthys crocea) (LcGCSFb) was cloned by RACE-PCR techniques. The open reading frame (ORF) of LcGCSFb is 603 bp long and encoded a protein precursor of 200 amino acids (aa), with a 19-aa signal peptide and a 181-aa mature peptide. In healthy fish, the LcGCSFb was constitutively expressed in all examined tissues, with the highest levels in mucous tissues, such as gills, intestine, and stomach. Its transcripts in head kidney, spleen, gills, intestine and stomach were significantly induced by Vibrio alginolyticus challenge. LcGCSFb transcripts were also detected in primary head kidney leukocytes (PKL), primary head kidney macrophages (PKM), primary head kidney granulocytes (PKG) and head kidney cell line (LYCK), and markedly upregulated by inactivated V. alginolyticus. These data suggested that LcGCSFb may play a role in immune response against bacterial infection. In vivo administration of recombinant LcGCSFb protein (rLcGCSFb) significantly upregulated the expression levels of the inflammatory cytokines (IL-6 and TNFα), and transcription factor C/EBPβ, which is required for proliferation of neutrophils. Furthermore, rLcGCSFb showed an ability to strengthen the phagocytosis of PKL in vitro. Taken together, LcGCSFb may be involved in antibacterial immunity via promoting the inflammatory response and the phagocytic activity of leukocytes. To our knowledge, this is the first report on immunoregulatory roles of GCSF in teleost.
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http://dx.doi.org/10.1016/j.fsi.2019.04.048DOI Listing
July 2019

Six types of tea reduce high-fat-diet-induced fat accumulation in mice by increasing lipid metabolism and suppressing inflammation.

Food Funct 2019 Apr;10(4):2061-2074

College of Horticulture, South China Agricultural University, Guangzhou 510000, China.

A high-fat diet results in obesity because of white fat accumulation. Although tea extracts alleviate lipid metabolism disorders and decrease white fat accumulation, the mechanisms underlying the therapeutic actions of different types of Chinese tea are unclear. We established a murine model of obesity by feeding mice with a high-fat diet (HFD) and treating them with atorvastatin (positive control) or water extracts (WEATs) of different tea types. The food and water intake, body weight gain, white fat accumulation, and triglyceride (TG) and total cholesterol (TC) levels were evaluated to assess the effects of the WEATs on obesity. The levels of the lipid metabolism enzymes p-AMPK, CPT-1A and FAS and the pro-inflammatory factors iNOS and IL-6 were determined. The WEATs not only reduced the body weight and white fat accumulation in the HFD-induced obese mice, but also relieved hepatic steatosis. Comparing the effects of the six kinds of tea showed that white tea has the best anti-obesity effect. Yellow tea and raw pu-erh tea significantly up-regulated p-AMPK, green tea, white tea and raw pu-erh tea markedly inhibited FAS, and white tea, yellow tea and oolong tea up-regulated CPT-1. Therefore, it is possible that white tea, yellow tea and oolong tea inhibit obesity by increasing energy expenditure and fatty acid oxidation, while green tea, white tea and raw pu-erh tea exert their effects by inhibiting fatty acid synthesis. In addition, the WEATs also significantly decreased the levels of IL-6, while green tea, yellow tea and oolong tea significantly inhibited iNOS. Different types of tea have specific chemical compositions and can regulate different lipid metabolism related proteins. In conclusion, despite variations in its composition and mechanism of action, tea is a potent anti-obesity agent.
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http://dx.doi.org/10.1039/c8fo02334dDOI Listing
April 2019

Health Risk Assessment of Inorganic Mercury and Methylmercury via Rice Consumption in the Urban City of Guiyang, Southwest China.

Int J Environ Res Public Health 2019 01 14;16(2). Epub 2019 Jan 14.

Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province, Guizhou Normal University, Guiyang 550001, China.

Rice consumption is the main methylmercury (MeHg) exposure route for residents in mercury (Hg) mining areas. However, there is limited studies on mercury in commercial rice, which has high liquidity and can be directly consumed by urban residents. This study measured the total Hg (THg) and MeHg concentrations in 146 rice samples purchased from the markets in Guiyang city, southwest China, and both the inorganic Hg (IHg) and MeHg estimated daily intakes (EDIs) and hazard quotients (HQs) were calculated according to rice consumption. The THg concentrations in all rice samples (range: 0.97 to 13.10 μg·kg; mean: 3.88 μg·kg) were lower than the Chinese national standard (20 μg·kg). The average MeHg concentration in rice was 1.16 μg·kg. The total HQs (THQs) ranged from 0.0106 to 0.1048, with a mean of 0.0462, which was far lower than 1. This result suggests that there were low Hg exposure levels through consumption of commercial rice in residents of Guiyang.
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http://dx.doi.org/10.3390/ijerph16020216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352273PMC
January 2019

An improved genome assembly for reveals hepcidin gene expansion with diversified regulation and function.

Commun Biol 2018 16;1:195. Epub 2018 Nov 16.

Institute of Oceanology, College of Animal Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China.

(large yellow croaker) is a type of perciform fish well known for its peculiar physiological properties and economic value. Here, we constructed an improved version of the genome assembly, which contained 26,100 protein-coding genes. Twenty-four pseudo-chromosomes of were also reconstructed, comprising 90% of the genome assembly. This improved assembly revealed several expansions in gene families associated with olfactory detection, detoxification, and innate immunity. Specifically, six hepcidin genes (LcHamps) were identified in , possibly resulting from lineage-specific gene duplication. All LcHamps possessed similar genomic structures and functional domains, but varied substantially with respect to expression pattern, transcriptional regulation, and biological function. LcHamp1 was associated specifically with iron metabolism, while LcHamp2s were functionally diverse, involving in antibacterial activity, antiviral activity, and regulation of intracellular iron metabolism. This functional diversity among gene copies may have allowed to adapt to diverse environmental conditions.
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http://dx.doi.org/10.1038/s42003-018-0207-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240063PMC
November 2018