Publications by authors named "Qiqi Huang"

12 Publications

  • Page 1 of 1

An L-Shaped Relationship Between Serum Iron and Stroke-Associated Pneumonia.

Clin Interv Aging 2021 22;16:505-511. Epub 2021 Mar 22.

Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, People's Republic of China.

Objective: Pneumonia is a common complication in patients with stroke. There was a close relationship between serum iron and inflammatory response. This study aimed to explore the relationship between serum iron levels and stroke-associated pneumonia (SAP).

Methods: Patients with acute stroke were recruited from the First Affiliated Hospital of Wenzhou Medical University and divided into SAP group and Non-SAP group. The demographic and clinical data of the patients were collected via the medical records, and the blood samples were collected within 24 hours after admission. The predictive value of serum iron to SAP was evaluated by receiver operating characteristic curve (ROC) and binary Logistic regression models. A restricted cubic spline (RCS) was used to furtherly clarify the relationship between serum iron and the risk of SAP.

Results: A total of 906 participants were enrolled, including Non-SAP group (n = 755) and SAP group (n = 151). Serum iron levels in the SAP group were significantly lower than those in the Non-SAP group (9.77±5.61 vs 14.01±6.80, P < 0.001). Logistic regression showed that patients with high serum iron levels (≥7.8μmol/L) showed a lower risk of SAP (OR=0.43, 95% CI, 0.27-0.69, P < 0.001). Besides, the RCS model showed that there was an L-shaped relationship between the serum iron and risk of SAP (P for non-linearity: 0.014).

Conclusion: Low serum iron level was a risk factor for SAP, and there was an L-shaped relationship between them. Stroke patients with low serum iron levels should be alert to the risk of SAP.
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http://dx.doi.org/10.2147/CIA.S301480DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997604PMC
March 2021

Flavonoids from Sophora alopecuroides L. improve palmitate-induced insulin resistance by inhibiting PTP1B activity in vitro.

Bioorg Med Chem Lett 2021 03 5;35:127775. Epub 2021 Jan 5.

National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun 130024, China. Electronic address:

Seventeen flavonoids (1-17) were isolated from Sophora alopecuroides L.. Compounds 1 and 2 were new compounds, and compounds 5, 8, 11, 12, and 17 were isolated from S. alopecuroides for the first time. The sources of compounds 1 and 2 were determined from the seeds of S. alopecuroides by UPLC-QE-Orbitrap-MS, and compounds 1, 2, 7, 13, 14, 15, 16, and 17 were proven to improve the insulin resistance of C2C12 myotubes and significantly increase glucose consumption levels. Among them, compounds 1, 2, 13, 14, 16, and 17 could bind to protein tyrosine phosphatase 1B (PTP1B), thereby significantly inhibiting the enzyme activity of PTP1B. Compound 2 had the strongest inhibitory effect, with an inhibition rate of 95.22% at 0.1 μg mL.
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http://dx.doi.org/10.1016/j.bmcl.2021.127775DOI Listing
March 2021

Whether the risk of gestational diabetes mellitus is affected by , , or polymorphisms: a meta-analysis.

Diabetol Metab Syndr 2020 17;12:81. Epub 2020 Sep 17.

Department of Endocrinology, Huzhou Hospital of Traditional Chinese Medicine, Zhejiang University of Traditional Chinese Medicine, No.315 of South Chaoyang Street, Huzhou, 313000 China.

Background: Whether polymorphisms in tumor necrosis factor-α (), interleukin-6 (), interleukin-10 () or adiponectin () influence the risk of gestational diabetes mellitus (GDM) or not remain inconclusive. Therefore, the authors conducted a meta-analysis to robustly assess relationships between polymorphisms in , , or and the risk of GDM by merging the results of eligible publications.

Methods: A through literature searching in Medline, Embase, Wanfang, VIP and CNKI was conducted by the authors to identify eligible publications, and twenty-two publications were finally found to be eligible for merged quantitative analyses.

Results: The merged quantitative analyses revealed that  + 45T/G (rs2241766) polymorphism was significantly associated with the risk of GDM in overall population (dominant comparison: OR = 0.70, p < 0.001; recessive comparison: OR = 1.95, p < 0.001; over-dominant comparison: OR = 1.18, p = 0.03; allele comparison: OR = 0.71, p < 0.001) and Asians (dominant comparison: OR = 0.70, p < 0.001; recessive comparison: OR = 1.94, p < 0.001; allele comparison: OR = 0.72, p < 0.001). Nevertheless, we did not observe any positive results for  - 238G/A (rs361525),  - 308G/A (rs1800629),  - 174G/C (rs1800795),  - 819C/T (rs1800871),  - 592C/A (rs1800872),  - 1082A/G (rs1800896) and  + 276G/T (rs1501299) polymorphisms.

Conclusions: The present meta-analysis shows that among investigated , , or polymorphisms only  + 45T/G (rs2241766) polymorphism may affect the risk of GDM.
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http://dx.doi.org/10.1186/s13098-020-00582-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499992PMC
September 2020

Encapsulation of emulsions by a novel delivery system of fluid core-hard shell biopolymer particles to retard lipid oxidation.

Food Funct 2020 Jul 23;11(7):5788-5798. Epub 2020 Jun 23.

Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.

Colloidal delivery systems could be designed to retard lipid oxidation in foods, thereby extending their shelf-lives and improving their nutritional quality. In this study, a class of novel fluid core-hard shell biopolymer particles with lipid droplets being encapsulated in the biopolymer has been designed and fabricated to increase their lipid oxidative stability. This was achieved by injecting a mixture of Tween 80-coated lipid droplets, xanthan gum, and calcium ions into a sodium alginate solution at either pH 3 or 7. The viscosity, hardness, microstructure, physical stability, and chemical stability of the droplet-loaded fluid core-hard shell (FCHS) biopolymer particles were then measured. The results indicated that the FCHS biopolymer particles had thinner, denser, and harder shells at pH 3 than at pH 7. The thickness of the alginate biopolymer particle walls could be modulated by varying the xanthan gum to alginate ratio used during fabrication. The lipid oxidation measurements indicated that the primary (PV) and secondary (TBARS) reaction products decreased by approximately 60% and 75%, respectively, compared to the control after 13 days of storage at pH 3. These results indicate that the encapsulation of lipid droplets within the FCHS biopolymer particles substantially increased their oxidative stability. The biopolymer particles developed in this study may have promising applications in various food, pharmaceutical, and cosmetic products for retarding lipid oxidation.
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http://dx.doi.org/10.1039/d0fo00725kDOI Listing
July 2020

The effect of night shift on sleep quality and depressive symptoms among Chinese nurses.

Neuropsychiatr Dis Treat 2019 7;15:435-440. Epub 2019 Feb 7.

Anji County People's Hospital, Huzhou 313300, China,

Purpose: Night shift is associated with adverse physical and psychological health outcomes such as poor sleep quality and depressive symptoms. We aimed to compare sleep quality as well as depressive symptoms in nurses working night shifts to those working day shifts only and explore the association between sleep quality and depressive symptoms among nurses.

Patients And Methods: Eight hundred sixty-five nurses were enrolled in the current study. Sleep quality and depressive symptoms among nurses were evaluated by the Pittsburgh Sleep Quality Index (PSQI) and Hospital Anxiety and Depressive Disorders Rating Scale (HADS), respectively.

Results: PSQI and HADS scores were both significantly higher in the nurses working night shifts (<0.05) than in those working day shifts only. Besides, there was a positive correlation between PSQI and HADS scores. Binary logistic regression showed that night shift and poor sleep quality were independent risk factors of depressive symptoms among nurses.

Conclusion: Higher rates of depression among Chinese nurses working night shifts may be associated with poor sleep quality induced by night shift.
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http://dx.doi.org/10.2147/NDT.S190689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369837PMC
February 2019

Development and characterization of 23 polymorphic microsatellite loci for (Taxaceae), a relict vulnerable species.

Appl Plant Sci 2018 May 23;6(5):e01149. Epub 2018 May 23.

School of Life Sciences Sun Yat-sen University Guangzhou 510275 People's Republic of China.

Premise Of The Study: New microsatellite markers were developed for the vulnerable conifer species (Taxaceae) to investigate population genetic variation and the effects of environmental heterogeneity on genetic structure.

Methods And Results: A total of 27 microsatellite loci were developed from through a Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, of which 23 were polymorphic. These markers yielded 1-13 alleles and 1.0-7.9 effective alleles per locus; levels of observed and expected heterozygosity varied from 0.000-1.000 and 0.000-0.873, respectively. In total, 18 of the markers were transferable to the related species .

Conclusions: These polymorphic markers are a valuable genetic resource for investigating population genetic variation and the potential for local adaptation in .
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http://dx.doi.org/10.1002/aps3.1149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991555PMC
May 2018

Stimulation of methane yield rate from food waste by aerobic pre-treatment.

Bioresour Technol 2018 Aug 9;261:279-287. Epub 2018 Apr 9.

Department of Bioscience and Biotechnology, Faculty of Agriculture Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

Aerobic pre-treatment (AP) was applied to enhance methane yield from food waste through anaerobic digestion. Different AP durations (i.e. 2, 5 and 8 days) prior to anaerobic digestion were tested. The results indicated that AP of food waste led to no significant differences (p > 0.05) in methane yield potential (ca. 418 mL/g-VS). However, a suitable AP duration (5 days) increased methane yield rates (ca. 18 mL/d/g-VS; 22.0% higher than the control) by anticipating methane generation and shortening the methanogenic phase via volatile fatty acid reduction and pH increase. Although AP induced chemical oxygen demand loss to some extent (i.e. by 2.6%-9.9%) in the AP stage via aerobic degradation, the methane yield potential could be recovered by enhancing organic matter hydrolysis. Therefore, maximisation of hydrolysis should be used as a basis for determining a suitable AP duration for various types of organic matter.
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http://dx.doi.org/10.1016/j.biortech.2018.04.006DOI Listing
August 2018

Effects of digestate recirculation on a two-stage anaerobic digestion system, particularly focusing on metabolite correlation analysis.

Bioresour Technol 2018 Mar 10;251:40-48. Epub 2017 Dec 10.

Department of Bioscience and Biotechnology, Faculty of Agriculture Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

Single-stage (S-N treatment) and two-stage anaerobic digestion with (T-R treatment) and without digestate recirculation (T-N treatment) for methane production using food waste (FW) were comparatively evaluated to examine the effects of digestate recirculation on anaerobic digestion (AD). Digestate recirculation positively affected the methane yield and organic loading rate (OLR). Metabolite correlation analysis revealed that a systematic hydrolysis degree of greater than 75% is crucial to achieve the complete recoverable yield of methane from FW. Digestate recirculation also markedly increased the system alkalinity, maintaining an optimum pH for methanogens. However, the ammonium accumulated by T-R treatment would destroy the metabolic balance between the hydrolytic bacteria and methanogens, especially at a critical OLR. Therefore, the appropriate control of two-stage AD systems with digestate recirculation is limited not only to OLR regulation but also to the prevention of ammonium accumulation.
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http://dx.doi.org/10.1016/j.biortech.2017.12.020DOI Listing
March 2018

A comprehensive review on food waste anaerobic digestion: Research updates and tendencies.

Bioresour Technol 2018 Jan 20;247:1069-1076. Epub 2017 Sep 20.

Advanced Environmental Biotechnology Centre, Nanyang Environment & Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.

Anaerobic digestion has been practically applied in agricultural and industrial waste treatment and recognized as an economical-effective way for food waste disposal. This paper presented an overview on the researches about anaerobic digestion of food waste. Technologies (e.g., pretreatment, co-digestion, inhibition and mitigation, anaerobic digestion systems, etc.) were introduced and evaluated on the basis of bibliometric analysis. Results indicated that ethanol and aerobic prefermentation were novel approaches to enhance substrates hydrolysis and methane yield. With the promotion of resource recovery, more attention should be paid to biorefinery technologies which can produce more useful products toward zero emissions. Furthermore, a technological route for food waste conversion based on anaerobic digestion was proposed.
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http://dx.doi.org/10.1016/j.biortech.2017.09.109DOI Listing
January 2018

The relationship between liver-kidney impairment and viral load after nephropathogenic infectious bronchitis virus infection in embryonic chickens.

Poult Sci 2017 Jun;96(6):1589-1597

To examine the relationship of impairments of the liver and kidney with viral load after nephropathogenic infectious bronchitis virus (NIBV) infection in embryonic chickens, 120 specific-pathogen-free Leghorn embryonated chicken eggs were randomly divided into two groups (infected and control), with three replicates per group and 20 eggs in each replicate. The eggs in the infected and control groups were challenged with 0.2 mL of 105.5 ELD50 NIBV and sterile saline solution, respectively. The embryonic chickens' plasma and liver and kidney tissues were collected at 1, 3, and 5 days post-inoculation (dpi), the liver and kidney functional parameters were quantified, and the tissue viral loads were determined with real-time PCR. The results showed that plasma potassium, sodium, chlorine, magnesium, calcium, and phosphorus levels were increased. The infected group exhibited significantly higher plasma uric acid, blood urea nitrogen, and creatinine levels than the control group at 3 dpi. The plasma concentrations of aspartate aminotransferase and alanine aminotransferase were significantly increased in the infected group. The total protein, albumin, and globulin levels in the infected group were significantly lower than those in the control group. The liver-kidney viral load in the infected group peaked at 3 dpi, at which time the kidney viral load was significantly higher than that of the liver. Our results indicated that NIBV infection caused liver and kidney damage in the embryonic chickens, and the results also demonstrated that the liver and kidney damage was strongly related to the tissue viral load following NIBV infection in embryonic chickens.
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http://dx.doi.org/10.3382/ps/pew455DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7107109PMC
June 2017

Prokaryotic expression of the chicken xanthine oxidase (XOD) subunit and its localization in liver and kidney.

Int J Biol Macromol 2016 Jun 4;87:341-7. Epub 2016 Mar 4.

Clinical Veterinary Laboratory, College of Animal Science and Technology, Jiangxi Agricultural University, Jiangxi, China.

Xanthine oxidase (XOD) is the members of the molybdenum hydroxylase flavoprotein family and it plays a vital role in the body's purine catabolism. In this study, we cloned the XOD 37kDa subunit protein by using RT-PCR and pMD-18-T clone vector based on the total RNA extracted from chicken liver. The cloning XOD subunit protein gene was ligated into the pET-32a to construct the recombinant plasmid pET-XOD. After the pET-XOD expression vector was transformed into host cells Rosetta (DE3), the recombinant XOD subunit proteins (54.8kDa) were successfully induced by isopropy1 β-d-thiogalactoside (IPTG). Rabbit antiserums were produced by using the purification of the recombinant XOD subunit protein as antigen. The titer of the antiserum was more than 1:102,400 determined by using ELISA. The result of Western blot demonstrated that the antiserum could specifically recognize the chicken liver XOD. Immunohistochemistry and immunofluorescence showed that the XOD mainly presented in the cytoplasm of chicken hepatocytes and proximal tubular epithelial cells. Our results indicated that the XOD subunit protein polyclonal antibody prepared by this method could be used for the further researches of the biological function of the XOD in the chicken.
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http://dx.doi.org/10.1016/j.ijbiomac.2016.03.001DOI Listing
June 2016

Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers.

J Vet Sci 2015 ;16(4):423-9

Clinical Veterinary Laboratory, College of Animal Science and Technology, Jiangxi Agricultural University, Jiangxi 330029, China.

To assess relationships between xanthine oxidase (XOD) and nephropathogenic infectious bronchitis virus (NIBV) infection, 240 growing layers (35 days old) were randomly divided into two groups (infected and control) of 120 chickens each. Each chicken in the control and infected group was intranasally inoculated with 0.2 mL sterile physiological saline and virus, respectively, after which serum antioxidant parameters and renal XOD mRNA expression in growing layers were evaluated at 8, 15 and 22 days post-inoculation (dpi). The results showed that serum glutathione peroxidase and superoxide dismutase activities in the infected group were significantly lower than in the control group at 8 and 15 dpi (p < 0.01), while serum malondialdehyde concentrations were significantly higher (p < 0.01). The serum uric acid was significantly higher than that of the control group at 15 dpi (p < 0.01). In addition, the kidney mRNA transcript level and serum activity of XOD in the infected group was significantly higher than that of the control group at 8, 15 and 22 dpi (p < 0.05). The results indicated that NIBV infection could cause the increases of renal XOD gene transcription and serum XOD activity, leading to hyperuricemia and reduction of antioxidants in the body.
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http://dx.doi.org/10.4142/jvs.2015.16.4.423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4701734PMC
September 2016