Publications by authors named "Qiong Wu"

1,761 Publications

  • Page 1 of 1

Identification and Characterization of Resistance of Three Aphid Species on Contrasting Alfalfa Cultivars.

Insects 2022 Jun 9;13(6). Epub 2022 Jun 9.

The First-Class Discipline of Prataculture Science of Ningxia University (No. NXYLXK2017A01), College of Agriculture, Ningxia University, Yinchuan 750021, China.

Aphids on alfalfa () including Koch (cowpea aphid, CPA), Harris (pea aphid, PA) and Buckton (spotted alfalfa aphid, SAA) cause significant yield losses worldwide. In this experiment, the development of these three species of aphids on 16 alfalfa cultivars was compared. The results showed that the plant cultivar had a significant influence on the development of aphids as there are significant differences in the body weight of aphids reared on different alfalfa cultivars. In addition, antibiosis between the alfalfa cultivars Pegasis and Gannong NO.9 and the three species of aphids was evaluated by measuring aphid body weight and fecundity. Antixenosis was measured using choice tests, and feeding behavior was quantified using electrical penetration graphs (EPG). The Pegasis cultivar was observed to have both antibiosis and antixenosis effects with CPA, but was susceptible to PA and SAA compared with the Gannong NO.9 cultivar. CPA had less mean body weight, less fecundity, and shorter feeding time on the Pegasis cultivar, and preferred to settle on Gannong NO.9 cultivar. In contrast, Gannong NO.9 exhibits antibiosis and antixenosis to PA and SAA compared with Pegasis, as shown by lower body weight, lower fecundity and chose to settle less often, but EPG data showed that PA and SAA showed no different significance in feeding behavior between Pegasis and Gannong NO.9.
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http://dx.doi.org/10.3390/insects13060530DOI Listing
June 2022

Comparison of the agreement between WeChat-based self-administered and interviewer-administered data on infant and young child feeding in China: A test-retest study.

J Glob Health 2022 Jun 20;12:11004. Epub 2022 Jun 20.

Nuffield Department of Primary Care Health Sciences, University of Oxford, Oxford, UK.

Background: Measuring infant and young child feeding (IYCF) indicators is important in evaluating child health programs and making evidence-based decisions. With Internet and new media rapidly developing, communication apps such as WeChat (the most popular mobile social media platform in China) are widely used and can potentially be used as an alternative way to collect infant and young child feeding information. This study compares data agreement between a WeChat-based self-administered and an interviewer-administered survey on infant and young child feeding information.

Methods: We recruited 297 mothers of children aged 6-23 months in Fenxi County, Shanxi Province, China. Using the Test-Retest method, we first collected data through a self-administered survey using a WeChat-based electronic questionnaire and asked 36 questions on breastfeeding and complementary feeding knowledge, practices, and information sources. We then conducted an interviewer-administered survey using the same questionnaire and compared the data agreement between the two survey methods during the same day. Cohen's kappa score (κ) and intraclass correlation coefficients (ICC) were used for data agreement analysis for all 36 questions and six key IYCF indicators. The McNemar test was used to identify differences between the two survey methods for the six key indicators.

Results: There was substantial or almost perfect agreement for 33 questions (κ/ICC>0.60), and slight or fair agreement for the other 3 questions (κ/ICC<0.40). Agreement of all six key IYCF indicators was substantial or almost perfect (κ = 0.78-0.94), while two indicators showed statistical differences between the two survey methods (P = 0.03 for "Minimum meal frequency" and P = 0.001 for "Minimum accepted diet"). Analysis of reasons for inconsistencies showed that 43.6% of all the inconsistencies were not caused by the self-administered survey method. The cost of the interviewer-administered survey was much higher than that of the self-administered survey: ¥45.9 (US$6.8) vs ¥19.7 (US$2.9) per questionnaire.

Conclusions: The WeChat-based self-administered method can be used for future data collection of infant and young child feeding information in China. Most of the questions and key indicators showed very good agreement without statistical differences between the two methods.
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http://dx.doi.org/10.7189/jogh.12.11004DOI Listing
June 2022

Frontal white matter association with sleep quality and the role of stress.

J Sleep Res 2022 Jun 14:e13669. Epub 2022 Jun 14.

Department of Psychiatry, Maryland Psychiatric Research Center, School of Medicine, University of Maryland, Baltimore, Maryland, USA.

An important measure of brain health is the integrity of white matter connectivity structures that link brain regions. Studies have found an association between poorer sleep quality and decreased white matter integrity. Stress is among the strongest predictors of sleep quality. This study aimed to evaluate the association between sleep quality and white matter and to test if the relationship persisted after accounting for stress. White matter microstructures were measured by diffusion tensor imaging in a population of Old Order Amish/Mennonite (N = 240). Sleep quality was determined by the Pittsburgh Sleep Quality Index. Current stress levels were measured by the perceived stress scale. Exposure to lifetime stress was measured by the lifetime stressor inventory. Microstructures of four white matter tracts: left and right anterior limbs of internal capsule, left anterior corona radiata, and genu of corpus callosum were significantly correlated with sleep quality (all p ≤ 0.001). The current stress level was a significant predictor of sleep quality (p ≤ 0.001) while lifetime stress was not. PSQI remained significantly associated with white matter integrity in these frontal tracts (all p < 0.01) after accounting for current stress and lifetime stress, while current and lifetime stress were not significant predictors of white matter in any of the four models. Sleep quality did not have any substantial mediation role between stress and white matter integrity. Sleep quality was significantly associated with several frontal white matter tracts that connect brain structures important for sleep regulation regardless of current or past stress levels.
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http://dx.doi.org/10.1111/jsr.13669DOI Listing
June 2022

Fast detection, a precise and sensitive diagnostic agent for breast cancer.

J Exp Clin Cancer Res 2022 Jun 13;41(1):201. Epub 2022 Jun 13.

School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China.

Background: Breast cancer targeting diagnostic agent with effective imaging ability is important in guiding plan formulation, prediction, and curative effect evaluation of tumors in clinic. A tumor-targeting nanoprobe based on the functional and programmable Liquid-Liquid phase separation of AS1411 promoted by Ru(II) complex RuPEP may develop into a potential phosphorescence probe to detect breast cancer cells, where AS1411 act as a tumor-targeting guidance moiety to distinguish tumor cells from normal cells and RuPEP act as a light-emitting element to highlight breast cancer cells.

Methods: Here we designed and constructed a nanoprobe [email protected], and the physicochemical and biochemical properties were characterized by TEM, AFM and EDS. The breast cancer targeting diagnostic capacity was evaluated by normal/tumor cell co-culture assay, tumor cells targeting tracking in xenograft model and cancerous area selectively distinguishing in human patient tissue.

Results: Further studies indicated that the nanoprobe exhibits excellent tumor-targeting imaging ability in vitro and in vivo by effectively recognize the over-expressed nucleolin (NCL) on the breast cancer cells membrane. Intriguingly, we discovered that the selectively enrichment of nanoprobe particles in tumor cells is related to ATP-dependent NCL transport processes that rely on the AS1411 component of nanoprobe to recognize NCL. Furthermore, preferential accumulation of nanoprobe is clearly differentiating the human breast cancer tissue surrounding non-cancerous tissue in histological analysis.

Conclusion: This study produce a potent nanoprobe can be used as a convenient tool to highlight and distinguish tumor cells in vivo, and indicate the tumorous grading and staging in human breast cancer patient pathological section, which provides an effective way for breast cancer diagnostic imaging by targeting recognize NCL.
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http://dx.doi.org/10.1186/s13046-022-02393-3DOI Listing
June 2022

Optimization of clofibrate with O-desmethyl anetholtrithione lead to a novel hypolipidemia compound with hepatoprotective effect.

Bioorg Med Chem Lett 2022 Jun 10;72:128844. Epub 2022 Jun 10.

Institute of Translational Medicine, Shanghai University 99 Shangda Road, Shanghai 200444, People's Republic of China. Electronic address:

Oxidative stress and inflammation were considered to be the major mechanisms in liver damage caused by clofibrate (CF). In order to obtain lipid-lowering drugs with less liver damage, the structure of clofibrate was optimized by O-desmethyl anetholtrithione and got the target compound clofibrate-O-desmethyl anetholtrithione (CF-ATT). CF-ATT significantly reduced the levels of plasma triglycerides (TG), total cholesterol (TC) in hyperlipidemia mice induced by Triton WR-1339. In addition, CF-ATT has a significantly protective effect on the liver compared with CF. The liver weight and liver coefficient were reduced. The hepatic function indexes were also decreased, such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). Histopathological examination of the liver revealed that inflammatory cell infiltration, nuclear degeneration, cytoplasmic loosening and hepatocyte necrosis were ameliorated by administration with CF-ATT. The hepatoprotective mechanism showed that CF-ATT significantly up-regulated Nrf2 and HO-1 protein expression and down-regulated p-NF-κB P65 expression in the liver. CF-ATT has obviously antioxidant and anti-inflammatory activity. These findings suggested that CF-ATT has significant hypolipidemia activity and exact hepatoprotective effect possibly through the Nrf2/NF-κB-mediated signal pathway.
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http://dx.doi.org/10.1016/j.bmcl.2022.128844DOI Listing
June 2022

Hepatitis B Virus X Protein Is Stabilized by the Deubiquitinating Enzyme VCPIP1 in a Ubiquitin-Independent Manner by Recruiting the 26S Proteasome Subunit PSMC3.

J Virol 2022 Jun 13:e0061122. Epub 2022 Jun 13.

Key Laboratory of Gastrointestinal Cancer (Fujian Medical University), Ministry of Education, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, China.

Hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide, and the viral X protein (HBx) is an etiological factor in HCC development. HBx is a high-turnover protein, but knowledge of the role of deubiquitinating enzymes (DUBs) in maintaining HBx homeostasis is very limited. We used a 74-DUB library-based yeast two-hybrid assay and determined that a novel DUB, valosin-containing protein-interacting protein 1 (VCPIP1), interacted with HBx. VCPIP1 and its C-terminal amino acids 863 to 1221 upregulated the HBx protein expression, with or without HBV infection. Mechanistically, VCPIP1 stabilized HBx protein through a ubiquitin-independent pathway, which was validated by the HBx ubiquitination site mutant plasmid. Coimmunoprecipitation assays demonstrated the potency of VCPIP1 in recruiting 26S proteasome regulatory subunit 6A (PSMC3) and forming a ternary complex with HBx through mutual interaction. , purified His-tagged PSMC3 protein rescued HBx degradation induced by the 20S proteasome, and VCPIP1 synergized the mechanism. Functionally, HBx specifically binding to VCPIP1 significantly enhanced the transcriptional transactivation of HBx by activating NF-κB, AP-1, and SP-1 and inhibited hepatoma cell clonogenicity in Huh7 and HepG2 cells. Moreover, we further demonstrated that overexpression of VCPIP1 significantly affected the HBV covalently closed circular DNA (cccDNA) transcription in HBV-infected HepG2-NTCP cells. Altogether, our results indicate a novel mechanism by which VCPIP1 recruits PSMC3 to bind with HBx, stabilizing it in a ubiquitin-independent manner, which might be critical for developing DUB inhibitors in the future. HBx is a multifunctional viral oncoprotein that plays an essential role in the viral life cycle and hepatocarcinogenesis. HBx degradation occurs through the ubiquitin-proteasome system (UPS). However, whether novel compartments of the DUBs in the UPS also act in regulating HBx stability is not fully understood. Here, for the first time, we defined VCPIP1 as a novel DUB for preventing HBx degradation by the 20S proteasome in a ubiquitin-independent manner. PSMC3, encoding the 26S proteasome regulatory subunit, directly stabilized HBx through physical binding instead of a common approach in protein degradation, serving as the key downstream effector of VCPIP1 on HBx. Therefore, the ternary binding pattern between VCPIP1, HBx, and PSMC3 is initiated for the first time, which eventually promotes HBx stability and its functions. Our findings provide novel insights into host-virus cross talk by targeting DUBs in the UPS.
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http://dx.doi.org/10.1128/jvi.00611-22DOI Listing
June 2022

Antibacterial Activity and Mechanism of GO/CuO/ZnO Coating on Ultrafine Glass Fiber.

Nanomaterials (Basel) 2022 May 29;12(11). Epub 2022 May 29.

International Laboratory for Insulation and Energy Efficiency Materials, College of Materials Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China.

A GO (graphene oxide)/ZnO/CuO antibacterial coating was successfully sprayed on the ultrafine glass fibers using room temperature hydrothermal synthesis and air spraying techniques. The microstructures of the antibacterial coating were characterized, and the results showed that the CuONPs (nano particles)/ZnONPs were uniformly dispersed on the surface of GO. Then, the antibacterial properties of the GO/ZnO/CuO (GZC) antibacterial coating were evaluated using the disc diffusion test. It was found that the coating exhibits excellent antibacterial properties and stability against and , and the antibacterial rate of each group of antibacterial powder against Staphylococcus aureus () and Escherichia coli () was 100%. To explore the antibacterial mechanism of the GZC antibacterial powder on the ultrafine glass fibers based on the photocatalysis/oxidative stress method, the photoelectric coupling synergistic effect between GZC antibacterial coating was analyzed deeply. The results all showed that the photochemical activity of GZC antibacterial powder was significantly improved compared with pure component materials. The enhancement of its photochemical activity is beneficial to the generation of ROS (including hydroxyl radicals, superoxide anion radicals, etc.), which further confirms the speculation of the photocatalytic/oxidative stress mechanism.
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http://dx.doi.org/10.3390/nano12111857DOI Listing
May 2022

Rigid Schiff Base Complex Supermolecular Aggregates as a High-Performance pH Probe: Study on the Enhancement of the Aggregation-Caused Quenching (ACQ) Effect via the Substitution of Halogen Atoms.

Int J Mol Sci 2022 Jun 2;23(11). Epub 2022 Jun 2.

Department of Chemical Science and Technology, Kunming University, Kunming 650214, China.

Optical signals of pH probes mainly driven from the formation or rupture of covalent bonds, whereas the changes in covalent bonds usually require higher chemical driving forces, resulting in limited sensitivity and reversibility of the probes. The exploration of high-performance pH probes has been a subject of intense investigation. Herein, a new pH probe has been developed, with optical property investigation suggesting the probe has excellent signal-to-noise ratio, and fluorescence intensity shows exponential growth, combined with a visible color change, as pH increased from 5.1 to 6.0; Moreover, the probe has outstanding stability and reversibility, with more than 90% of the initial signal intensity remaining after 30 cycles. In order to better understand the special fluorescence behavior of the reported probe, the non-halogenated isomer is introduced for comparison, combined with the results of structural analysis, quantitative calculation and optical experiments, and the possible mechanism of the special supramolecular aggregation-caused quenching effect induced by the halogen atom is discussed.
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http://dx.doi.org/10.3390/ijms23116259DOI Listing
June 2022

Impact of Low-Temperature Storage on the Microstructure, Digestibility, and Absorption Capacity of Cooked Rice.

Foods 2022 Jun 2;11(11). Epub 2022 Jun 2.

School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

This study examined the effects of low-temperature storage on the microstructural, absorptive, and digestive properties of cooked rice. Cooked rice was refrigerated and stored at 4 °C for 0.5, 1, 3, 5, and 7 days, as well as frozen and preserved at -20, -40, and -80 °C for 0.5, 1, 3, 5, 7, 14, 21, and 28 days. The results indicated that the stored rice samples generally exhibited a higher absorption capacity for oil, cholesterol, and glucose than the freshly cooked rice. In addition, after storage, the digestibility of the cooked rice declined, namely, the rapidly digestible starch (RDS) content and estimated glycemic index (eGI) decreased, whereas the slowly digestible starch (SDS) and resistant starch (RS) content increased. Moreover, the increment of the storage temperatures or the extension of storage periods led to a lower amylolysis efficiency. Scanning electron microscopy (SEM) analysis indicated that storage temperature and duration could effectively modify the micromorphology of the stored rice samples and their digestion. Moreover, microstructural differences after storage and during simulated intestinal digestion could be correlated to the variations in the absorption capacity and digestibility. The findings from this study will be useful in providing alternative storage procedures to prepare rice products with improved nutritional qualities and functional properties.
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http://dx.doi.org/10.3390/foods11111642DOI Listing
June 2022

meGPS: a multi-omics signature for hepatocellular carcinoma detection integrating methylome and transcriptome data.

Bioinformatics 2022 Jun 8. Epub 2022 Jun 8.

Shenzhen People's Hospital, First Affiliated Hospital of Southern University of Science and Technology, Second Clinical Medicine College of Jinan University, Shenzhen, 518020, China.

Motivation: Hepatocellular carcinoma (HCC) is a primary malignancy with poor prognosis. Recently, multi-omics molecular-level measurement enables HCC diagnosis and prognosis prediction, which is crucial for early intervention of personalized therapy to diminish mortality. Here, we introduce a novel strategy utilizing DNA methylation and RNA expression data to achieve a multi-omics gene pair signature (GPS) for HCC discrimination.

Results: The immune genes with negative correlations between expression and promoter methylation are enriched in the highly connected cancer-related pathway network, which are considered as the candidates for HCC detection. After that, we separately construct a methylation GPS (mGPS) and an expression GPS (eGPS), and then assemble them as a meGPS with five gene pairs, in which the significant methylation and expression changes occur between HCC tumor and non-tumor groups. Reliable performance has been validated by independent tissue (age, gender, and etiology) and blood datasets. This study proposes a procedure for multi-omics GPS identification and develops a novel HCC signature using both methylome and transcriptome data, suggesting potential molecular targets for the detection and therapy of HCC.

Availability And Implementation: Models are available at https://github.com/bioinformaticStudy/meGPS.git.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btac379DOI Listing
June 2022

Generation of MiRNA sponge constructs targeting multiple MiRNAs.

J Clin Lab Anal 2022 Jun 6:e24527. Epub 2022 Jun 6.

Zhejiang Provincial Key Laboratory of Pathophysiology, Medical School, Ningbo University, Ningbo, China.

Background: MicroRNAs(miRNAs) are key regulators of gene expression in plants, animals and some viruses. Hence, alteration of miRNA levels in cells or tissues is common for miRNA studies. Loss-of-function of miRNA can be achieved using antisense oligonucleotides, sponges and gene knockout models.

Methods: Here, we showed an efficient, rational and economical way to construct multi-targeted miRNA sponges with desired copies. Four copies of miRNA sponge are used as "building-blocks".

Results: These building-blocks, which can target same miRNA or different miRNAs, are linked together through ligation. Each time of ligation can double the number of sponge copies.

Conclusions: In this way, we constructed lentivirus vectors harboring sponges targeting miR-21, miR-31 and miR-155 and the combination of two miRNA sponges can inhibit cancer cell growth significantly.
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http://dx.doi.org/10.1002/jcla.24527DOI Listing
June 2022

C-reactive protein and white blood cell are associated with frailty progression: a longitudinal study.

Immun Ageing 2022 Jun 3;19(1):29. Epub 2022 Jun 3.

Department of Epidemiology & Biostatistics, and Department of Respiratory Diseases of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, Zhejiang, China.

Background: Systemic inflammation has been linked to diseases and frailty. However, little is known about the effect of systemic inflammation on frailty progression with a longitudinal study design.

Objectives: This study aimed to investigate the associations of two inflammation indicators, C-reactive protein (CRP) and white blood cell (WBC), with frailty progression.

Methods: This study utilized data from the China Health and Retirement Longitudinal Study 2011-2018 (wave 1-wave 4). Frailty index (FI) was calculated using 40 items from wave 1 to wave 4 (range: 0 to 1). Two systemic inflammation biomarkers, CRP and WBC, were measured at baseline (wave 1) and logs transformed as continuous variables or grouped using quartiles. Linear mixed-effect models were used to analyze the associations of these two biomarkers with the progression of frailty with adjustment for potential confounding factors.

Results: The study enrolled 9111 middle-aged and older participants (52.7% females, mean age 58.8 ± 9.3 years). The median follow-up time was 7.0 years. In a fully adjusted model with further adjustment for baseline FI, higher CRP (β for the interaction with time = 0.239, 95% CI: 0.139 to 0.338) and WBC (β for the interaction with time = 0.425, 95% CI: 0.024 to 0.825) significantly accelerated the rate of increase in the FI during the follow-up period. The associations were more pronounced in younger people (< 60 years) than older people (≥60 years).

Conclusions: Higher CRP and WBC accelerated the progression of frailty, particularly in younger groups (< 60 years). The findings suggest the importance of systemic inflammation for the early identification of people at high risk of rapid progression of frailty.
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http://dx.doi.org/10.1186/s12979-022-00280-1DOI Listing
June 2022

DHW-221, a Dual PI3K/mTOR Inhibitor, Overcomes Multidrug Resistance by Targeting P-Glycoprotein (P-gp/ABCB1) and Akt-Mediated FOXO3a Nuclear Translocation in Non-small Cell Lung Cancer.

Front Oncol 2022 13;12:873649. Epub 2022 May 13.

Department of Life Science and Biochemistry, Shenyang Pharmaceutical University, Shenyang, China.

Multidrug resistance (MDR) is considered as a primary hindrance for paclitaxel failure in non-small cell lung cancer (NSCLC) patients, in which P-glycoprotein (P-gp) is overexpressed and the PI3K/Akt signaling pathway is dysregulated. Previously, we designed and synthesized DHW-221, a dual PI3K/mTOR inhibitor, which exerts a remarkable antitumor potency in NSCLC cells, but its effects and underlying mechanisms in resistant NSCLC cells remain unknown. Here, we reported for the first time that DHW-221 had favorable antiproliferative activity and suppressed cell migration and invasion in A549/Taxol cells and . Importantly, DHW-221 acted as a P-gp inhibitor binding to P-gp, which resulted in decreased P-gp expression and function. A mechanistic study revealed that the DHW-221-induced FOXO3a nuclear translocation Akt inhibition was involved in mitochondrial apoptosis and G0/G1 cell cycle arrest only in A549/Taxol cells and not in A549 cells. Interestingly, we observed that high-concentration DHW-221 reinforced the pro-paraptotic effect stimulating endoplasmic reticulum (ER) stress and the mitogen-activated protein kinase (MAPK) pathway. Additionally, intragastrically administrated DHW-221 generated superior potency without obvious toxicity FOXO3a nuclear translocation in an orthotopic A549/Taxol tumor mouse model. In conclusion, these results demonstrated that DHW-221, as a novel P-gp inhibitor, represents a prospective therapeutic candidate to overcome MDR in Taxol-resistant NSCLC treatment.
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http://dx.doi.org/10.3389/fonc.2022.873649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9137409PMC
May 2022

B81 Bone Vibrator-Induced Vestibular-Evoked Myogenic Potentials: Normal Values and the Effect of Age.

Front Neurol 2022 11;13:881682. Epub 2022 May 11.

Department of Otorhinolaryngology, Head and Neck Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Objective: To define the normal values and examine the influence of aging on B81 bone vibrator-induced cervical vestibular-evoked myogenic potentials (B81-cVEMPs) and ocular vestibular-evoked myogenic potentials (B81-oVEMPs).

Methods: Seventy healthy subjects, divided into seven groups according to their ages, were enrolled in this study. The 4-9-, 10-19-, 20-29-, 30-39-, 40-49-, 50-59-, and 60-70-year-old participants were divided into groups I-VII, respectively. B81-cVEMP and B81-oVEMP were recorded in each group.

Results: The B81-cVEMP response rates for groups I-VII were 100, 100, 100, 100, 95, 95, and 75%, respectively, with significant differences only between groups I-VI and group VII ( = 0.047, < 0.05). The B81-oVEMP response rates for groups I-VII were 100, 100, 100, 100, 70, 65, and 40%, respectively, with significant differences only between groups I-IV and groups V-VII ( = 0.020, = 0.008, = 0.000; < 0.05). The threshold, P13, and N23 latencies of B81-cVEMP positively correlated with age (r = 0.756, = 0.000; r = 0.357, = 0.003; r = 0.316, = 0.009; < 0.05). The raw amplitudes and corrected amplitudes negatively correlated with age (r = -0.641, = 0.000; r = -0.609, = 0.000, < 0.05). For B81-oVEMP, the corrected amplitudes negatively correlated with age (r = -0.638, = 0.000, <0.05), but the threshold and N10 latency positively correlated with age (r = 0.768, = 0.000; r = 0.334, = 0.009, < 0.05). Moreover, the interaural asymmetry ratio did not significantly correlate with age for B81-cVEMP and B81-oVEMP.

Conclusion: As age increased, the B81-cVEMP response rate decreased, the thresholds increased, P13 and N23 latencies were prolonged, and the raw amplitude and corrected amplitude decreased. The B81-oVEMP response rate and corrected amplitude decreased, the thresholds increased, and N10 latency was prolonged with age. These changes are probably due to the occurrence of morphological and functional changes in the vestibular system with aging. Therefore, we suggest establishing different reference values according to different age groups when evaluating the VEMP results in patients with vestibular diseases.
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http://dx.doi.org/10.3389/fneur.2022.881682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131004PMC
May 2022

Arene Ru(II) Complexes Acted as Potential G-Quadruplex DNA Stabilizer Induced DNA Damage Mediated Apoptosis to Inhibit Breast Cancer Progress.

Molecules 2022 May 10;27(10). Epub 2022 May 10.

School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.

A series of arene Ru(II) complexes, [(-MeCH)Ru(L)Cl]Cl, (L=-ClPIP, ; -ClPIP, and -ClPIP, ) (-ClPIP=2-(2-chlorophenyl)imidazo[4,5-f][1,10]phenanthroline; -ClPIP=2-(3-chlorophenyl)imidazo[4,5-f][1,10]phenanthroline; -ClPIP=2-(4-chlorophenyl)imidazo[4,5-f][1,10]phenanthroline) was synthesized and investigated as a potential apoptosis inducer in chemotherapy. Spectroscopy and molecular docking simulations show that exhibits moderated binding affinity to G-quadruplex DNA by groove mode. Further, in vitro studies reveal that displays inhibitory activity against MCF-7 growth with IC = 3.7 ± 0.2 μM. Flow cytometric analysis, comet assay, and immunofluorescence confirm that can induce the apoptosis of MCF-7 cells and G0/G1 phase arrest through DNA damage. In summary, the prepared arene Ru(II) complexes can be developed as a promising candidate for targeting G-quadruplex structure to induce the apoptosis of breast cancer cells via binding and stabilizing G-quadruplex conformation on oncogene promoter.
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http://dx.doi.org/10.3390/molecules27103046DOI Listing
May 2022

Highly sensitive and selective surface plasmon resonance biosensor for the detection of SARS-CoV-2 spike S1 protein.

Analyst 2022 Jun 13;147(12):2809-2818. Epub 2022 Jun 13.

Department of Thoracic Surgery, The First Hospital of Jilin University, 71 Xinmin Street, Changchun 130021, Jilin, China.

The reality that the coronavirus disease 2019 (COVID-19) is still raging around the world and making a comeback with a strong presence has highlighted the need for rapid and sensitive quantitative detection methods of viral RNA, antibody and antigen for widespread tracking and screening applications. Surface plasmon resonance (SPR) detection technology has achieved rapid development and become a standard measurement method in the fields of biosensing, biomedicine, biochemistry and biopharmaceuticals due to its advantages of high sensitivity, fast response and no need for labelling. Here, we report a sandwiched structure-based SPR biosensor for detecting a specific viral antigen, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike S1 protein. The sensor combines a TiC-MXene nanosheet modified sensing platform and polydopamine (PDA)-Ag nanoparticle (AgNP)/anti-SARS-CoV-2 spike S1 protein nanoconjugate signal enhancers, exhibiting a wide linear range of 0.0001 to 1000 ng mL with a low detection limit of 12 fg mL (S/N = 3). In the analysis of artificial saliva and human serum samples, the proposed SPR biosensor exhibits good reproducibility and high specificity, which indicates its potential for application in complex bodily fluids. The exploitation of the MXene-based SPR biochip for recognizing the SARS-CoV-2 antigen provides an accessible and rapid way for COVID-19 diagnosis, and promotes the application of 2D nanomaterial-based sensing chips in clinical diagnosis and disease screening. Significantly, the proposed method possesses general applicability that can be reprogrammed to detect any protein antigen if a corresponding specific nanobody is available.
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http://dx.doi.org/10.1039/d2an00426gDOI Listing
June 2022

Identification of prognostic alternative splicing signature in gastric cancer.

Arch Public Health 2022 May 25;80(1):145. Epub 2022 May 25.

The Cancer Institute, Tangshan People's Hospital, Tangshan, 063001, China.

Background: Aberrant alternative splicing (AS) events could be viewed as prognostic indicators in a large number of malignancies. This study aims to identify prognostic AS events, illuminate the function of the splicing variants biomarkers and provide reliable evidence for formulating public health strategies for gastric cancer (GC) surveillance.

Methods: RNA-Seq data, clinical information and percent spliced in (PSI) values were available in The cancer genome atlas (TCGA) and TCGA SpliceSeq data portal. A three-step regression method was conducted to identify prognostic AS events and construct multi-AS-based signatures. The associations between prognostic AS events and splicing factors were also investigated.

Results: We identified a total of 1,318 survival-related AS events in GC, parent genes of which were implicated in numerous oncogenic pathways. The final prognostic signatures stratified by seven types of AS events or not stratified performed well in risk prediction for GC patients. Moreover, five signatures based on AA, AD, AT, ES and RI events function as independent prognostic indicators after multivariate adjustment of other clinical variables. Splicing network also showed marked correlation between the expression of splicing factors and PSI value of AS events in GC patients.

Conclusion: Our findings provide a landscape of AS events and regulatory network in GC, indicating that AS events might serve as prognostic biomarkers and therapeutic targets for GC.
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http://dx.doi.org/10.1186/s13690-022-00894-3DOI Listing
May 2022

Ordered micropattern arrays fabricated by lung-derived dECM hydrogels for chemotherapeutic drug screening.

Mater Today Bio 2022 Jun 5;15:100274. Epub 2022 May 5.

Institute of Respiratory Health, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.

Aims: This study aims to evaluate ECM-coated micropattern arrays derived from decellularization of native porcine lungs as a novel three-dimensional cell culture platform.

Methods: ECM derived from decellularization of native porcine lungs was exploited to prepare hydrogels. Then, dECM-coated micropattern arrays were fabricated at four different diameters (50, 100, 150 and 200 ​μm) using polydimethylsiloxane (PDMS). Two lung cancer cell lines, A549 and H1299, were tested on a dECM-coated micropattern array as a novel culture platform for cell adhesion, distribution, proliferation, viability, phenotype expression, and drug screening to evaluate the cytotoxicity of paclitaxel, doxorubicin and cisplatin.

Results: The ECM derived from decellularization of native porcine lungs supported cell adhesion, distribution, viability and proliferation better than collagen I and Matrigel as the coated matrix on the surface. Moreover, the optimal diameter of the micropattern arrays was 100-150 ​μm, as determined by measuring the morphology, viability, proliferation and phenotype of the cancer cell spheroids. Cell spheroids of A549 and H1299 on dECM-coated micropattern arrays showed chemoresistance to anticancer drugs compared to that of the monolayer. The different distributions of HIF-1α, MCL-1 (in the center) and Ki-67 and MRP2 (in the periphery) of the spheroids demonstrated the good establishment of basal-lateral polarity and explained the chemoresistance phenomenon of spheroids.

Conclusions: This novel three-dimensional cell culture platform is stable and reliable for anticancer drug testing. Drug screening in dECM-coated micropattern arrays provides a powerful alternative to existing methods for drug testing and metabolic profiling in the drug discovery process.
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http://dx.doi.org/10.1016/j.mtbio.2022.100274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114688PMC
June 2022

m6A methylated EphA2 and VEGFA through IGF2BP2/3 regulation promotes vasculogenic mimicry in colorectal cancer via PI3K/AKT and ERK1/2 signaling.

Cell Death Dis 2022 May 21;13(5):483. Epub 2022 May 21.

School of Life Science and Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150001, Heilongjiang, China.

Exploring the epigenetic regulation mechanism of colorectal cancer (CRC) from the perspective of N6-methyladenosine (m6A) modification may provide a new target for tumor therapy. Analysis using high-throughput RNA-seq profile from TCGA found that the gene expression of Methyltransferase-like 3 (METTL3) was significantly upregulated among 20 m6A binding proteins in CRC, which was also validated in CRC cancer tissues and cell lines. Moreover, transcriptome sequencing in METTL3 knockdown cells using CRISPR/Cas9 editing suggested that EphA2 and VEGFA were differential expression, which were enriched in the vasculature development, PI3K/AKT and ERK1/2 signal pathway through the functional enrichment analysis. The results in vitro revealed that METTL3 as the m6A "writers" participates the methylation of EphA2 and VEGFA, which were recognized by the m6A "readers", insulin-like growth factor 2 mRNA binding protein 2/3 (IGF2BP2/3), to prevent their mRNA degradation. In addition, EphA2 and VEGFA targeted by METTL3 via different IGF2BP-dependent mechanisms were found to promote vasculogenic mimicry (VM) formation via PI3K/AKT/mTOR and ERK1/2 signaling in CRC. The study suggests that intervention with m6A-binding proteins (METTL3 and IGF2BP2/3) may provide a potential diagnostic or prognostic target of VM-based anti-metastasis drugs for CRC.
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http://dx.doi.org/10.1038/s41419-022-04950-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122982PMC
May 2022

A core-shell liquid metal-Cu nanoparticle with glutathione consumption an replacement strategy for tumor combination treatment of chemodynamic, microwave dynamic and microwave thermal therapy.

Biomater Sci 2022 May 20. Epub 2022 May 20.

Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

The presence of high content glutathione (GSH) provides an effective "protective shield" for tumor cells, which undoubtedly is a huge impediment to reactive oxygen species (ROS)-based treatment. Fortunately, divalent copper (Cu) can not only consume GSH, destroying the protection mechanism of GSH, but also can be reduced to Cu with excellent Fenton-like reaction activity. Hence, capitalizing on the properties of liquid metals, we introduced Cu with three different valances an replacement reaction. A stable core-shell liquid-metal based "Cu storage pool" was obtained. It can effectively deplete GSH within the cells, and simultaneously produce ·OH through a Fenton-like reaction, further improving the effect of chemodynamic therapy (CDT). Under microwave irradiation, it is also capable of producing a large amount of ROS to promote tumor treatment. In addition, the loading of ionic liquid endows [email protected] nanoparticles with certain microwave heating performance, which is able to augment microwave thermal therapy (MWTT). With the combination of CDT, microwave dynamic therapy (MDT) and MWTT, [email protected] has an excellent effect on tumor elimination. This work offers a new idea for the application of liquid metals and the combined treatment of tumors, which has potential application value.
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http://dx.doi.org/10.1039/d2bm00435fDOI Listing
May 2022

Layer-Dependent Interlayer Antiferromagnetic Spin Reorientation in Air-Stable Semiconductor CrSBr.

ACS Nano 2022 May 19. Epub 2022 May 19.

Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371.

Magnetic van der Waals (vdW) materials possess versatile spin configurations stabilized in reduced dimensions. One magnetic order is the interlayer antiferromagnetism in A-type vdW antiferromagnet, which may be effectively modified by the magnetic field, stacking order, and thickness scaling. However, atomically revealing the interlayer spin orientation in the vdW antiferromagnet is highly challenging, because most of the material candidates exhibit an insulating ground state or instability in ambient conditions. Here, we report the layer-dependent interlayer antiferromagnetic spin reorientation in air-stable semiconductor CrSBr using magnetotransport characterization and first-principles calculations. We reveal an odd-even layer effect of interlayer spin reorientation, which originates from the competitions among interlayer exchange, magnetic anisotropy energy, and extra Zeeman energy of uncompensated magnetization. Furthermore, we quantitatively constructed the layer-dependent magnetic phase diagram with the help of a linear-chain model. Our work uncovers the layer-dependent interlayer antiferromagnetic spin reorientation engineered by magnetic field in the air-stable semiconductor.
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http://dx.doi.org/10.1021/acsnano.2c01151DOI Listing
May 2022

Tension experience induced by tonal and melodic shift at music phrase boundaries.

Sci Rep 2022 May 18;12(1):8304. Epub 2022 May 18.

CAS Key Laboratory of Behavioral Science, Institute of Psychology, Chinese Academy of Sciences, Beijing, China.

Music tension is a link between music structures and emotions. As music unfolds, developmental patterns induce various emotional experiences, but the relationship between developmental patterns and tension experience remains unclear. The present study compared two developmental patterns of two successive phrases (tonal shift and melodic shift) with repetition condition to investigate the relationship with tension experience. Professional musicians rated on-line felt tension and EEG responses were recorded while listening to music sequences. Behavioral results showed that tension ratings under tonal and melodic shift conditions were higher than those under repetition conditions. ERP results showed larger potentials at early P300 and late positive component (LPC) time windows under tonal shift condition, and early right anterior negativity (ERAN) and LPC under melodic shift condition. ERSP results showed early beta and late gamma power increased under tonal shift condition, theta power decreased and alpha power increased under melodic shift condition. Our findings suggest that developmental patterns play a vital role in tension experiences; tonal shift affects tension by tonal shift detection and integration, while melodic shift affects tension by attentional processing and working memory integration. From the perspective of Event Structure Processing Model, solid evidence was given to specify the time-span segmentation and reduction.
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http://dx.doi.org/10.1038/s41598-022-11949-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117266PMC
May 2022

DZW-310, a novel phosphoinositide 3-kinase inhibitor, attenuates the angiogenesis and growth of hepatocellular carcinoma cells via PI3K/AKT/mTOR axis.

Biochem Pharmacol 2022 Jul 14;201:115093. Epub 2022 May 14.

Department of Life Science and Biochemistry, Shenyang Pharmaceutical University, Shenyang, Liaoning Province 110016, PR China; Department of Pharmacy, General Hospital of Northern Theater Command, Shenyang, Liaoning Province 110016, PR China. Electronic address:

Hepatocellular carcinoma (HCC) is one of the most malignant tumors worldwide with high lethality and prevalence. The deregulated phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) pathway plays an indispensable role in mediating the progression of HCC. Among them, PI3K acts as the most pivotal initiator, contributing to multiple malignant biological processes, like proliferation, apoptosis and angiogenesis. Many PI3K inhibitors (PI3Kis) have been proved or proceeded into clinical as antineoplastic drugs. Nevertheless, the application of PI3Kis for the treatment of HCC remains a blank. Accordingly, our study identified a novel PI3Ki (DZW-310) with strong anti-HCC activity in vitro and in vivo. This study aimed to evaluate its anti-HCC effect and elucidate its potential mechanism. Our current results revealed that DZW-310 significantly attenuated HCC cell growth through promoting intrinsic apoptosis and G0/G1 phase cell arrest. Moreover, DZW-310 suppressed angiogenesis by regulating the HIF-1α/VEGFA axis. Further mechanistic investigation demonstrated that DZW-310, functioned as a PI3Ki, exerted strong anti-HCC activity by acting on PI3Kα (a major subtype of PI3K) and ulteriorly deactivating the PI3K/AKT/mTOR pathway. Collectively, our studies identified that DZW-310 is expected to become a promising HCC therapeutic agent and broaden clinical application of PI3Ki in HCC chemotherapy.
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http://dx.doi.org/10.1016/j.bcp.2022.115093DOI Listing
July 2022

AENEAS: A Randomized Phase III Trial of Aumolertinib Versus Gefitinib as First-Line Therapy for Locally Advanced or Metastatic Non-Small-Cell Lung Cancer With Exon 19 Deletion or L858R Mutations.

J Clin Oncol 2022 May 17:JCO2102641. Epub 2022 May 17.

Respiratory Medicine, Xiangya Hospital Central South University, Changsha, China.

Purpose: Aumolertinib (formerly almonertinib; HS-10296) is a novel third-generation epidermal growth factor receptor tyrosine kinase inhibitor approved in China. This double-blind phase III trial evaluated the efficacy and safety of aumolertinib compared with gefitinib as a first-line treatment for locally advanced or metastatic -mutated non-small-cell lung cancer (NSCLC; ClinicalTrials.gov identifier: NCT03849768).

Methods: Patients at 53 sites in China were randomly assigned 1:1 to receive either aumolertinib (110 mg) or gefitinib (250 mg) once daily. The primary end point was progression-free survival (PFS) per investigator assessment.

Results: A total of 429 patients who were naïve to treatment for locally advanced or metastatic NSCLC were enrolled. PFS was significantly longer with aumolertinib compared with gefitinib (hazard ratio, 0.46; 95% CI, 0.36 to 0.60; < .0001). The median PFS with aumolertinib was 19.3 months (95% CI, 17.8 to 20.8) versus 9.9 months with gefitinib (95% CI, 8.3 to 12.6). Objective response rate and disease control rate were similar in the aumolertinib and gefitinib groups (objective response rate, 73.8% and 72.1%, respectively; disease control rate, 93.0% and 96.7%, respectively). The median duration of response was 18.1 months (95% CI, 15.2 to not applicable) with aumolertinib versus 8.3 months (95% CI, 6.9 to 11.1) with gefitinib. Adverse events of grade ≥ 3 severity (any cause) were observed in 36.4% and 35.8% of patients in the aumolertinib and gefitinib groups, respectively. Rash and diarrhea (any grade) were observed in 23.4% and 16.4% of patients who received aumolertinib compared with 41.4% and 35.8% of those who received gefitinib, respectively.

Conclusion: Aumolertinib is a well-tolerated third-generation epidermal growth factor receptor tyrosine kinase inhibitor that could serve as a treatment option for -mutant NSCLC in the first-line setting.
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http://dx.doi.org/10.1200/JCO.21.02641DOI Listing
May 2022

Pyrimidine-Based Fluorescent Probe for Monitoring Mitophagy via Detection of Mitochondrial pH Variation.

Chembiochem 2022 May 16:e202200217. Epub 2022 May 16.

Key Laboratory of Flexible Electronics (KLOFE) &, Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), Nanjing, 211800, China.

Impaired mitophagy hinders the clearance of damaged mitochondria, inducing pathological states. Knowledge of this phenomenon is key to diagnosing certain diseases and understanding their pathogenesis. Mitophagy involves an acidization process that could serve as an ideal detection target. In this work, we designed and synthesized a mitochondrial-targeting fluorescence probe, Z2, for evaluating pH variation. This probe exhibited remarkable "turn-on" fluorescence under acidic conditions. In biological applications, Z2 showed a strong, specific pH detection capacity in Parkin-overexpressing HeLa cells during the mitophagy process. The "turn-on" fluorescence property of Z2 was also used to detect pH variations in Caenorhabditis elegans. This probe, as a novel pH assessment tool, may facilitate further research of mitophagy-associated pathological patterns.
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http://dx.doi.org/10.1002/cbic.202200217DOI Listing
May 2022

Antinociceptive diterpenoid alkaloids from the roots of .

J Asian Nat Prod Res 2022 May 16:1-7. Epub 2022 May 16.

Laboratory of Basic Medicine, General Hospital of Northern Theatre Command, Shenyang 110016, China.

A phytochemical investigation on the roots of afforded three undescribed aconitine-type C-diterpenoid alkaloids, austroyunnanines A-C (1-3). Structural elucidation of all the compounds were performed by spectral methods such as 1 D and 2 D (H-H COSY, HMQC, and HMBC) NMR spectroscopy. The isolated alkaloids were tested for their antinociceptive properties. Consequently, austroyunnanine B (2) exhibited significant antinociceptive effect and its ID value (48.0 μmol/kg) was 2-fold less than those of the positive control drugs aspirin and acetaminophen.
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http://dx.doi.org/10.1080/10286020.2022.2070483DOI Listing
May 2022

Comparative Genomics Revealed Wide Intra-Species Genetic Heterogeneity and Lineage-Specific Genes of .

Microbiol Spectr 2022 May 10:e0243921. Epub 2022 May 10.

Key Laboratory of Dairy Biotechnology and Engineering (IMAU), Ministry of Education; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering; Collaborative Innovative Center of Ministry of Education for Lactic Acid Bacteria and Fermented Dairy Products, Inner Mongolia Agricultural Universitygrid.411638.9, Hohhot, China.

Akkermansia muciniphila has potential as a next-generation probiotic, but few previous studies attempted to analyze its intraspecies population diversity. In this study, we performed a comparative genomic analysis of 112 filtered genomes from the NCBI database. The populations formed three clades (A-C) on the phylogenetic tree, suggesting the existence of three genetic lineages though clades B and C were phylogenetically closer than clade A. The three clades also showed geographic-based clustering, different genetic characteristics, and clade-specific genes. Two putative functional genes ( and ) were specific to clade C due to genomic islands. These lineage-specific genes might be associated with differences in genomic features (number of phages/genomic islands, pan-core genome, recombination rate, genetic diversity) between genetic lineages. The carbohydrate utilization gene profile (particularly for glycolytic hydrolases and carbohydrate esterases) also varied between clades, suggesting different carbohydrate metabolism potential/requirements between genetic lineages. Our findings provide important implications for future research on . Akkermansia muciniphila has been widely accepted as part of the next generation of probiotics. However, most current studies on have focused on the application of type strain BAA835 in the treatment of diseases, while few studies have reported on the genomic specificity, population structure, and functional characteristics of species. By comparing the genomes of 112 strains from NCBI which met the quality control conditions, we found that the population could be divided into three main clades (clades A to C) and presented a certain regional aggregation. There are significant differences among the three clades in their genetic characteristics and functional genes (the type strain BAA835 was located in clade A), especially in genes related to carbohydrate metabolism. It should be mentioned that probiotics should be a concept at the strain level rather than at the gut species level, so the probiotic properties of need to be carefully interpreted.
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http://dx.doi.org/10.1128/spectrum.02439-21DOI Listing
May 2022

Severe immune-related hepatitis and myocarditis caused by PD-1 inhibitors in the treatment of triple-negative breast cancer: a case report.

Ann Transl Med 2022 Apr;10(7):424

Department of Radiation Oncology, First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Triple-negative breast cancer (TNBC) is a subtype of breast cancer that is defined clinically by the absence of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (Her-2) expression. Due to its unresponsiveness to endocrine or targeted therapies, the clinical prognosis of TNBC is very poor. In recent years, research into immune checkpoint inhibitors (ICIs) has led to significant progress in the treatment of TNBC. A large amount of immune-related adverse events (irAEs), including skin, gastrointestinal, pulmonary, hepatic, cardiovascular, renal and endocrine adverse events, occur in clinical application owing to the widespread use of programmed death-1 (PD-1) inhibitors, although it is rarer to experience two irAEs simultaneously. The simultaneous occurrence of two irAEs increases the difficulty of diagnosis and treatment.

Case Description: In this case report, a 51-year-old TNBC woman with a background of modified radical mastectomy 3 years ago was diagnosed with axillary lymph node metastasis. This postoperative recurrent TNBC patient received chemotherapy combined with PD-1 inhibitors, resulting in severe immune-related hepatitis and myocarditis. The patient resolved after treatment with methylprednisolone, interruption of chemotherapy, and discontinuation of immunotherapy. The dosage of methylprednisolone gradually reduced from 80 to 4 mg/day with the improvement of liver function and cardiac function. She completed chemotherapy after recovering from irAEs and followed up for stable disease (SD) until 1st March 2021.

Conclusions: With the widespread use of ICIs, the incidence of irAEs has also increased. Early detection and treatment of irAEs presents a new challenge to clinicians. It is the first case report about two severe irAEs in postoperative recurrent TNBC patient after received chemotherapy combined with PD-1 inhibitors. This case illustrates the severe toxicity caused by ICIs which suggests more attention should be paid to early prevention, early diagnosis, and appropriate management of irAEs. Multidisciplinary discussions should be undertaken to improve patient prognosis.
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http://dx.doi.org/10.21037/atm-22-1284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073793PMC
April 2022

Identification of From the Skin Ulcer Disease of Crocodile Lizards () and Probiotics as the Control Measure.

Front Vet Sci 2022 21;9:850684. Epub 2022 Apr 21.

Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guilin, China.

The crocodile lizard () is an endangered ancient reptile species. Captive breeding is an important conservation measure for the potential restoration and recovery of their wild populations. However, a skin ulcer disease caused by an unknown pathogen has become a serious threat to captive breeding individuals. In the current study, based on microbial isolation, we identified as the dominant pathogen in skin ulcer disease. Chinese skinks () were used to verify the pathogenicity of in skin ulcer disease . As expected, subcutaneous inoculation of induced skin disease in healthy skinks and was re-isolated from the induced skin ulcers. Therefore, , an opportunistic and ubiquitous pathogen that causes a wide range of infections, appears to be the main pathogen of the skin disease affecting crocodile lizards. In the aquaculture industry, probiotics are widely used in the prevention and control of animal diseases caused by such pathogens. Here, we administered probiotics to the breeding crocodile lizards for 6 months. The three experiment groups treated with different kinds of probiotics showed significance at controlling case incidence. Three of the four groups treated with probiotics showed significant disease prevention (Effective Microorganisms mixed probiotics = 0.0374; Double-dose Effective Microorganisms, = 0.0299; = 0.0140, -test), and CFUs in the water of the breeding enclosures were also inhibited after probiotics usage ( < 0.001, -test). Our study demonstrated the role of in development of skin ulcer disease of crocodile lizards in a local zoo and offered the probiotic-based method for control measurements, which would be of benefit for the conservation of endangered reptiles.
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http://dx.doi.org/10.3389/fvets.2022.850684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069141PMC
April 2022

Identification of a Five-miRNA Signature for Diagnosis of Kidney Renal Clear Cell Carcinoma.

Front Genet 2022 20;13:857411. Epub 2022 Apr 20.

School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.

Kidney renal clear cell carcinoma, which is a common type and accounts for 70-80% of renal cell carcinoma, can easily lead to metastasis and even death. A reliable signature for diagnosis of this cancer is in need. Hence, we seek to select miRNAs for identifying kidney renal clear cell carcinoma. A feature selection strategy is used and improved to identify microRNAs for diagnosis of kidney renal clear cell carcinoma. Samples representing kidney renal clear cell carcinoma and normal tissues are split into training and testing groups. Accumulated scores representing the variable importance of each miRNA are derived from an iteration of resampling, training, and scoring. Those miRNAs with higher scores are selected based on the Gaussian mixture model. The sample split is repeated ten times to get more central miRNAs. A total of 611 samples are downloaded from TCGA, each of which contains 1,343 miRNAs. The improved feature selection method is implemented, and five miRNAs are identified as a biomarker for diagnosis of kidney renal clear cell carcinoma. GSE151419 and GSE151423 are selected as the independent testing sets. Experimental results indicate the effectiveness of the selected signature. Both data-driven measurements and knowledge-driven evidence are given to show the effectiveness of our selection results.
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http://dx.doi.org/10.3389/fgene.2022.857411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068871PMC
April 2022
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