Publications by authors named "Qiong Li"

428 Publications

Comparisons of topsoil geochemical elements from Northwest China and eastern Tibetan Plateau identify the plateau interior as Tibetan dust source.

Sci Total Environ 2021 Jul 22;798:149240. Epub 2021 Jul 22.

Key Laboratory of Western China's Environmental Systems (Ministry of Education), College of Earth and Environmental Sciences, Lanzhou University, Lanzhou 730000, China.

The high-altitude Tibetan Plateau (TP) is a potential substantial source of dust that could have major effects on the global dust cycle and lead to environmental change. Aeolian loess on the TP is a valuable archive for studying the history of dust activities. Identification of TP dust sources will deepen understanding of dust transport and atmospheric circulation patterns and their environmental impacts. However, consensus on the source of TP loess has not been reached, which hinders understanding of dust transport and paleoenvironmental explanations. In this study, topsoil and loess samples from Northwest (NW) China and eastern TP were used to systematically investigate the spatial variability in geochemical composition, and the element characteristics of the TP loess were used to identify its dust source. Compared with samples from NW China, the eastern TP topsoil and loess were relatively enriched in TiO, MnO, Cr, Co, Y, Zr, Nb, La, Ce, and Hf, but relatively depleted in NaO, MgO, CaO, Sr, and Pb. The geochemical composition and source analyses indicated that the dust from the Qaidam Basin, Hexi Corridor, and Tarim Basin in NW China was not the main contributing source to the TP loess. However, the elemental characteristics of the TP loess were broadly consistent, and element contents were similar to those of major and trace elements in surface sediments on the TP. Thus, the eastern TP loess was sourced primarily from the TP interior, revealing the TP as an important dust source in Asia.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149240DOI Listing
July 2021

Application of serum pepsinogen and carbohydrate antigen 72-4 (CA72-4) combined with gastrin-17 (G-17) detection in the screening, diagnosis, and evaluation of early gastric cancer.

J Gastrointest Oncol 2021 Jun;12(3):1042-1048

Department of Preventive Treatment, the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fujian, China.

Background: Gastric cancer is a common malignant tumor. The aim of the present study was to analyze the application value of serum pepsinogen (PG), carbohydrate antigen 72-4 (CA72-4), and gastrin-17 (G-17) detection in the screening, diagnosis, and evaluation of early gastric cancer.

Methods: In total, 122 patients with gastric cancer treated in our hospital from January 2018 to January 2021 were selected as the gastric cancer group and subdivided into the early gastric cancer (group A) and advanced gastric cancer (group B) groups. Sixty-five patients with benign gastric disease treated in the same hospital during the same period were selected as the control group, and 122 healthy people who underwent physical examination during the same period were allocated to the control group. The differences in the levels of G-17, PGI, PGII, PGI/PGII, and CA72-4 were compared; receiver-operating characteristic curves were drawn; and the efficacy of different factors in the diagnosis of early gastric cancer was calculated.

Results: G-17, PGI, and PGI/PGII levels in the gastric cancer group were significantly lower than those in the healthy group, and CA72-4 was significantly higher than that in the healthy group (P<0.05), but there was no significant difference in PGII between the 2 groups (P>0.05). G-17, PGI, and PGI/PGII levels in groups A and B were significantly lower than those in the control group. CA72-4 in groups A and B was significantly higher than that of the control group, and was highest in group B (P<0.05). The areas under the curve (AUC) of G-17, PGI, PGI/PGII, and CA72-4 were 0.671, 0.726, 0.769, and 0.602, respectively, and the AUC of combined detection was 0.883, which was significantly higher than that of single detection.

Conclusions: Serum PG, CA72-4 combined with G-17 detection has high sensitivity and specificity in the screening and diagnosis of early gastric cancer, and has high clinical application value.
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http://dx.doi.org/10.21037/jgo-21-254DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261308PMC
June 2021

Favored outgroup can eliminate the ingroup reference effect.

Scand J Psychol 2021 Jul 21. Epub 2021 Jul 21.

School of Psychology, Shaanxi Normal University, Xi'an, China.

Studies have found that, consistent with the ingroup favoritism effect, the recollection of ingroup related information is better than that of outgroup related information. This study explores whether preferred outgroup can eliminate the ingroup reference effect. Using the university as the reference group, Experiment 1 was designed to verify the group reference effect when there was no outgroup favoritism, and Experiment 2 examined the impact of outgroup favoritism on the group-reference effect. Experiment 1 found that recognition rates in the ingroup-reference condition were significantly higher than in the outgroup reference condition. Experiment 2 found no significant difference between recognition rates in the ingroup- reference and outgroup-reference conditions. Using household registration to classify the reference group, Experiment 3 recruited participants from rural areas to verify the group-reference effect while Experiment 4 recruited students from rural areas with a preference toward urban areas to explore the impact of outgroup favoritism on the group-reference effect. The results are the same as those in the Experiment 1 and Experiment 2. These findings suggest that the ingroup reference effect disappears when outgroups are preferred groups.
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http://dx.doi.org/10.1111/sjop.12767DOI Listing
July 2021

Impact of social class on health: The mediating role of health self-management.

PLoS One 2021 16;16(7):e0254692. Epub 2021 Jul 16.

School of Health, Wuhan Sports University, Wuhan, People's Republic of China.

Background: Studies have explored the relationship between social class and health for decades. However, the underlying mechanism between the two remains not fully understood. This study aimed to explore whether health self-management had a mediating role between social class and health under the framework of Socio-cultural Self Model.

Methods: 663 adults, randomly sampled from six communities in Southwest China, completed the survey for this study. Social class was assessed using individuals' income, education, occupation. Health self-management was assessed through evaluation of the health self-management behavior, health self-management cognition, health self-management environment. Physical health and mental health were measured by the Chinese version of Short-Form (36-item) Health Survey, which contains Physical Functioning, Role-Physical, Role-Emotional, Vitality, Mental Health, Social Function, Bodily Pain and General Health. Pearson's correlation was used to examine the associations between major variables. Mediation analyses were performed to explore the mediating role of health self-management.

Results: Social class positively predicted self-rated health. The lower the social class, the lower the self-reported physical and mental health. Health self-management partially mediated the relationship between social class and self-rated health. That is, the health self-management ability of the lower class, such as access to healthy and nutritious food and evaluate their own health status, is worse than that of the higher class, which leads to physical and mental health inequality between the high and the low classes.

Conclusion: Health self-management mediated the relationship between social class and health. Promoting health self-management abilities are conducive to improving both physical and mental health.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254692PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284807PMC
July 2021

Insight into diatom frustule structures using various imaging techniques.

Sci Rep 2021 Jul 15;11(1):14555. Epub 2021 Jul 15.

Faculty of Mechanical Engineering, Bialystok University of Technology, Białystok, Poland.

The diatom shell is an example of complex siliceous structure which is a suitable model to demonstrate the process of digging into the third dimension using modern visualization techniques. This paper demonstrates importance of a comprehensive multi-length scale approach to the bio-structures/materials with the usage of state-of-the-art imaging techniques. Imaging of diatoms applying visible light, electron and X-ray microscopy provide a deeper insight into the morphology of their frustules.
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http://dx.doi.org/10.1038/s41598-021-94069-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282634PMC
July 2021

Whole exome sequencing of six Chinese families with hereditary non-syndromic hearing loss.

Int J Pediatr Otorhinolaryngol 2021 Jul 7;148:110817. Epub 2021 Jul 7.

Department of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi'an, China. Electronic address:

Objectives: Hereditary non-syndromic hearing loss (NSHL) has a high genetic heterogeneity with about 152 genes identified as associated molecular causes. The present study aimed to detect the possible damaging variants of the deaf probands from six unrelated Chinese families.

Methods: After excluding the pathogenic/likely pathogenic variants in the most common genes, GJB2 and SLC26A4, 12 probands with prelingual deafness and autosomal recessive inheritance were evaluated by whole-exome sequencing (WES). All the candidate variants were verified by Sanger sequencing in all patients and their parents.

Results: Biallelic variants were identified in all deaf patients. Among these six families, 10 potentially causative variants, including 3 reported and 7 novel variants, in 3 different deafness-associated autosomal recessive (DFNB) genes (MYO15A, COL11A2, and CDH23) were identified. These novel variants are thought to be pathogenic or likely pathogenic for theirs predicted damage function upon the protein as while as cosegregated with the deafness phenotype. The variants in MYO15A were frequent with 7/10 candidate ones.

Conclusion: Next-generation sequencing (NGS) approach becomes more cost-effective and efficient when analyzing large-scale genes compared to the conventional polymerase chain reaction-based Sanger sequencing, which is often used to screen common deafness-related genes. The current findings further extend the pathogenic/likely pathogenic variants spectrum of hearing loss in the Chinese population, which has a positive significance for genetic counseling.
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http://dx.doi.org/10.1016/j.ijporl.2021.110817DOI Listing
July 2021

The Application of in Breast Cancer and the Mechanism of Its Main Component Nobiletin: A Systematic Review.

Evid Based Complement Alternat Med 2021 29;2021:2847466. Epub 2021 Jun 29.

Department of Traditional Chinese Medicine, Shanghai Jiao Tong University School of Medicine Affiliated Ruijin Hospital, Shanghai 200025, China.

and its main ingredient nobiletin (NOB) have received widespread attention in recent years due to their antitumor effects. The antitumor effect of is related to the traditional use, mainly in its Chinese medicinal properties of soothing the liver and promoting qi, resolving phlegm, and dispelling stagnation. Some studies have proved that and NOB are more effective for triple-negative breast cancer (TNBC), which is related to the syndrome of stagnation of liver qi. From the perspective of modern biomedical research, NOB has anticancer effects. Its potential molecular mechanisms include inhibition of the cell cycle, induction of apoptosis, and inhibition of angiogenesis, invasion, and migration. and NOB can also reduce the side effects of chemotherapy drugs and reverse multidrug resistance (MDR). However, more research studies are needed to clarify the underlying mechanisms. The modern evidence of and NOB in breast cancer treatment has a strong connection with the traditional concepts and laws of applying in Chinese medicine (CM). As a low-toxic anticancer drug candidate, NOB and its structural changes, , and compound prescriptions will attract scientists to use advanced technologies such as genomics, proteomics, and metabolomics to study its potential anticancer effects and mechanisms. On the contrary, there are relatively few studies on the anticancer effects of and NOB . The clinical application of and NOB as new cancer treatment drugs requires verification and further anticancer mechanism research. This review aims to provide reference for the treatment of breast cancer by Chinese medicine.
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http://dx.doi.org/10.1155/2021/2847466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260297PMC
June 2021

Newborn Screening and Genetic Analysis Identify Six Novel Genetic Variants for Primary Carnitine Deficiency in Ningbo Area, China.

Front Genet 2021 24;12:686137. Epub 2021 Jun 24.

Department of Gynaecology, Ningbo Women and Children's Hospital, Ningbo, China.

Primary carnitine deficiency (PCD) is an autosomal recessive disorder that could result in sudden death. It is caused by a defect in the carnitine transporter encoded by (Solute Carrier Family 22 Member 5, MIM:603377). Currently, a number of variants in have been identified, however, the PCD prevalence and its variants in Ningbo area are unclear. In this study, we screened 265,524 newborns by using tandem mass spectrometry. Variants in were further detected by next-generation sequencing in individuals with abnormal free carnitine levels (C0). We identified 53 newborns with abnormal C0 levels and 26 with variants in Among them, 16 with compound heterozygous or homozygous variants in were diagnosed with PCD, suggesting the PCD birth prevalence in Ningbo city was 1/16,595. Moreover, the C0 level was significantly ( = 0.013) higher in PCD patients than in those with one variant. Besides, the c.1400C > G (p. S467C) and c.51C > G (p. F17L) variants were the most frequent and six novel variants are all predicted to be damaging. This study reports the largest PCD patients in Ningbo area by newborn screening and expands the variant spectrum of . Our findings demonstrate the clinical value of combining NBS program results with DNA analysis for the diagnosis of PCD.
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http://dx.doi.org/10.3389/fgene.2021.686137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264545PMC
June 2021

Ruyiping extract reduces lung metastasis in triple negative breast cancer by regulating macrophage polarization.

Biomed Pharmacother 2021 Jul 8;141:111883. Epub 2021 Jul 8.

Institute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. Electronic address:

Lung metastasis of Triple-negative breast cancer (TNBC) causes severe breath-related events and poor prognosis. Ruyiping (RYP), a traditional Chinese medicine prescription, is used to treat breast cancer lung metastasis in clinical practice. This study was to explore the anti-lung-metastatic activities and mechanism of RYP extract by regulating macrophage polarization. The results showed that RYP can inhibit the viability and induce the apoptosis of TNBC cells. In in vitro experiments, RYP significantly inhibited the invasion and migration ability of TNBC cells promoted by M2, the subtype of macrophage which increased TNBC metastasis related genes. In in vivo experiments, RYP reduced the TNBC progression and lung metastasis. M2/M1 ration in the lung and M2 in the tumor was reduced by RYP, as well as M2 master regulator Stat6. Therefore, RYP extract may exhibit anti-lung metastasis function by reducing M2 in both tumor and lung through reducing Stat6.
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http://dx.doi.org/10.1016/j.biopha.2021.111883DOI Listing
July 2021

Design of fast response Doppler spectroscopy system for HUST field-reversed configuration device.

Rev Sci Instrum 2021 Jun;92(6):063522

International Joint Research Laboratory of Magnetic Confinement Fusion and Plasma Physics, State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

A fast response Doppler spectroscopy system with high throughput and high resolution is under development for studying the drifting velocity, rotation velocity, and ion temperature on the HUST field-reversed configuration (HFRC) device. The system has been designed to observe the spectral line of oxygen V (O V) ion emission at 278.1 nm (1s2s3p → 1s2s3s) over the lifetime (∼0.5 ms) of the FRC plasma. A high throughput Czerny-Turner monochromator with 3600 g/mm grating and 670 mm focal length is applied to achieve high spectral resolution; a 32-channel multi-anode photomultiplier tube (PMT) detector is utilized to achieve the high-speed response of up to 1 MHz; a 1D magnification optics combined with a cylindrical lens assembly and a fiber optic expansion is developed to magnify the spectral dispersion fitting the PMT channel interval. Through the ray-tracing analysis, the system's final spectral resolution is evaluated to be ∼0.03 nm. Taking into account the system sensitivity and O V line emissivity in HFRC plasma, the system is expected to be workable with the temporal response of 1 µs.
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http://dx.doi.org/10.1063/5.0043310DOI Listing
June 2021

High-dose Chemotherapy Combined with Autologous Hematopoietic Stem Cell Transplantation as Frontline Therapy for Intermediate/High-risk Diffuse Large B Cell Lymphoma.

Curr Med Sci 2021 Jun 3;41(3):465-473. Epub 2021 Jul 3.

Medical Center of Hematology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, China.

The role of autologous hematopoietic stem cell transplantation (auto-HSCT) following high-dose chemotherapy has been validated and accepted as a standard treatment for patients with relapsed diffuse large B-cell lymphoma (DLBCL). However, its clinical efficacy as frontline therapy remains to be elucidated. This study aimed to examine the feasibility of frontline auto-HSCT for newly diagnosed intermediate/high-risk DLBCL patients. We retrospectively reviewed the data of 223 patients treated with frontline auto-HSCT or chemotherapy alone (year 2008-2014) from four hospitals. The median follow-up time was 29.4 months. Between the two treatment arms among the intermediate/high-risk DLBCL patients, the 3-year overall survival (OS) and progression-free survival (PFS) rates of patients given frontline auto-HSCT were 87.6% and 81.9%, respectively, and the chemotherapy-alone group showed 3-year OS and PFS rates of 64.9% and 59.59%, respectively. Compared with the chemotherapy-alone group, the frontline auto-HSCT could eliminate the adverse impact of non-germinal center B-cell (GCB) type. In addition, in the frontline auto-HSCT group, patients who achieved complete response (CR) at auto-HSCT had a longer survival time than those who did not achieve CR. Our results suggested that frontline auto-HSCT could improve the prognosis of intermediate/high-risk DLBCL patients.
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http://dx.doi.org/10.1007/s11596-021-2394-2DOI Listing
June 2021

Morphology and Mechanical Properties of Fossil Diatom Frustules from Genera of and .

Nanomaterials (Basel) 2021 Jun 20;11(6). Epub 2021 Jun 20.

Fraunhofer Institute for Ceramic Technologies and Systems IKTS, Maria-Reiche-Str. 2, 01109 Dresden, Germany.

Fossil frustules of and were studied using laboratory-based nano X-ray tomography (nano-XCT), transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS). Three-dimensional (3D) morphology characterization using nondestructive nano-XCT reveals the continuous connection of fultoportulae, tube processes and protrusions. The study confirms that is different from . Both genera reveal heavily silicified frustules with valve faces linking together and forming cylindrical chains. For this cylindrical architecture of both genera, valve face thickness, mantle wall thickness and copulae thickness change with the cylindrical diameter. Furthermore, EDS reveals that these fossil frustules contain Si and O only, with no other elements in the percentage concentration range. Nanopores with a diameter of approximately 15 nm were detected inside the biosilica of both genera using TEM. In situ micromechanical experiments with uniaxial loading were carried out within the nano-XCT on these fossil frustules to determine the maximal loading force under compression and to describe the fracture behavior. The fracture force of both genera is correlated to the dimension of the fossil frustules. The results from in situ mechanical tests show that the crack initiation starts either at very thin features or at linking structures of the frustules.
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http://dx.doi.org/10.3390/nano11061615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235678PMC
June 2021

Resveratrol reverses hippocampal synaptic markers injury and SIRT1 inhibition against developmental Pb exposure.

Brain Res 2021 Jun 24;1767:147567. Epub 2021 Jun 24.

Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan Province, China; Environment and Health Innovation Team, College of Public Health, Zhengzhou University, Zhengzhou, Henan Province 450001, China. Electronic address:

Lead (Pb) exposure damages synaptic structural plasticity that results in cognitive impairment. Resveratrol, a natural polyphenolic compound, is one of the most potent agonists of silencing information regulator 1 (SIRT1) discovered to date. However, the effects of SIRT1 on synaptic functional plasticity in early life Pb exposure are not well studied. Herein, the purpose of this study is to investigate the expression of synaptic markers and SIRT1 in rats exposed to Pb and to evaluate the regulatory effect of resveratrol during this process. The Pb exposed male SD pups were treated with resveratrol (50 mg/kg/d) or EDTA (150 mg/kg/d) followed by hippocampal and blood sampling for analysis at postnatal day 21 (PND21). In the Morrris water maze test, resveratrol treatement protected the rats against Pb-induced impairment of learning and memory (P < 0.05). Resveratrol also enhanced the expression of brain-derived neurotrophic factor (BDNF, P < 0.001 vs 0.2% Pb group), and reversed the effects of Pb exposure on SIRT1(P < 0.001 vs 0.2% Pb group). The DG, CA1 and CA3 regions of the hippocampus showed a considerable increase in the expression of pre- and postsynaptic proteins (P < 0.001 vs 0.2% Pb group). In conclusion, our study demonstrated that resveratrol, through the activation of SIRT1, played a protective role against Pb-induced defects in synaptic plasticity, and suggested a new potential adjuvant treatment for Pb poisoning.
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http://dx.doi.org/10.1016/j.brainres.2021.147567DOI Listing
June 2021

Tailoring electrocatalytic activity of in situ crafted perovskite oxide nanocrystals via size and dopant control.

Proc Natl Acad Sci U S A 2021 Jun;118(25)

School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA 30332;

Perovskite oxides (ABO) have been widely recognized as a class of promising noble-metal-free electrocatalysts due to their unique compositional flexibility and structural stability. Surprisingly, investigation into their size-dependent electrocatalytic properties, in particular barium titanate (BaTiO), has been comparatively few and limited in scope. Herein, we report the scrutiny of size- and dopant-dependent oxygen reduction reaction (ORR) activities of an array of judiciously designed pristine BaTiO and doped BaTiO (i.e., La- and Co-doped) nanoparticles (NPs). Specifically, a robust nanoreactor strategy, based on amphiphilic star-like diblock copolymers, is employed to synthesize a set of hydrophobic polymer-ligated uniform BaTiO NPs of different sizes (≤20 nm) and controlled compositions. Quite intriguingly, the ORR activities are found to progressively decrease with the increasing size of BaTiO NPs. Notably, La- and Co-doped BaTiO NPs display markedly improved ORR performance over the pristine counterpart. This can be attributed to the reduced limiting barrier imposed by the formation of -OOH species during ORR due to enhanced adsorption energy of intermediates and the possibly increased conductivity as a result of change in the electronic states as revealed by our density functional theory-based first-principles calculations. Going beyond BaTiO NPs, a variety of other ABO NPs with tunable sizes and compositions may be readily accessible by exploiting our amphiphilic star-like diblock copolymer nanoreactor strategy. They could in turn provide a unique platform for both fundamental and practical studies on a suite of physical properties (dielectric, piezoelectric, electrostrictive, catalytic, etc.) contingent upon their dimensions and compositions.
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http://dx.doi.org/10.1073/pnas.2014086118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237576PMC
June 2021

CD73 Mesenchymal Stem Cells Ameliorate Myocardial Infarction by Promoting Angiogenesis.

Front Cell Dev Biol 2021 12;9:637239. Epub 2021 May 12.

Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, China.

With multipotent differentiation potential and paracrine capacity, mesenchymal stem cells (MSCs) have been widely applied in clinical practice for the treatment of ischemic heart disease. MSCs are a heterogeneous population and the specific population of MSCs may exhibit a selective ability for tissue repair. The aim of our research was to adapt the CD73 subgroup of adipose derived MSCs (AD-MSCs) for the therapy of myocardial infarction (MI). In this research, AD-MSCs were isolated from adipose tissue surrounding the groin of mice and CD73 AD-MSCs were sorted using flow cytometry. To investigate the therapeutic effects of CD73 AD-MSCs, 1.2 × 10 CD73 AD-MSCs were transplanted into rat model of MI, and CD73 AD-MSCs, normal AD-MSCs transplantation served as control. Our results revealed that CD73 AD-MSCs played a more effective role in the acceleration function of cardiac recovery by promoting angiogenesis in a rat model of MI compared with mixed AD-MSCs and CD73 AD-MSCs. Moreover, with the expression of CD73 in AD-MSCs, the secretion of VEGF, SDF-1α, and HGF factors could be promoted. It also shows differences between CD73 and CD73 AD-MSCs when the transcription profiles of these two subgroups were compared, especially in VEGF pathway. These findings raise an attractive outlook on CD73 AD-MSCs as a dominant subgroup for treating MI-induced myocardial injury. CD73, a surface marker, can be used as a MSCs cell quality control for the recovery of MI by accelerating angiogenesis.
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http://dx.doi.org/10.3389/fcell.2021.637239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152667PMC
May 2021

Amidation-Modified Apelin-13 Regulates PPAR and Perilipin to Inhibit Adipogenic Differentiation and Promote Lipolysis.

Bioinorg Chem Appl 2021 7;2021:3594630. Epub 2021 May 7.

Department of Endocrinology, The First Affiliated Hospital, Changsha Medical University, Changsha, Hunan 410219, China.

With the adjustment of human diet and lifestyle changes, the prevalence of obesity is increasing year by year. Obesity is closely related to the excessive accumulation of white adipose tissue (WAT), which can synthesize and secrete a variety of adipokines. Apelin is a biologically active peptide in the adipokines family. Past studies have shown that apelin plays an important regulatory role in the pathogenesis and pathophysiology of diseases such as the cardiovascular system, respiratory system, digestive system, nervous system, and endocrine system. Apelin is also closely related to diabetes and obesity. Therefore, we anticipate that apelin-13 has an effect on lipometabolism and intend to explore the effect of apelin-13 on lipometabolism at the cellular and animal levels. In in vitro experiments, amidation-modified apelin-13 can significantly reduce the lipid content; TG content; and the expression of PPAR, perilipin mRNA, and protein in adipocytes. Animal experiments also show that amidation modification apelin-13 can improve the abnormal biochemical indicators of diet-induced obesity (DOI) rats and can reduce the average diameter of adipocytes in adipose tissue, the concentration of glycerol, and the expression of PPAR and perilipin mRNA and protein. Our results show that apelin-13 can affect the metabolism of adipose tissue, inhibit adipogenic differentiation of adipocytes, promote lipolysis, and thereby improve obesity. The mechanism may be regulating the expression of PPAR to inhibit adipogenic differentiation and regulating the expression of perilipin to promote lipolysis. This study helps us understand the role of apelin-13 in adipose tissue and provide a basis for the elucidation of the regulation mechanism of lipometabolism and the development of antiobesity drugs.
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http://dx.doi.org/10.1155/2021/3594630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123992PMC
May 2021

Establishment and performance evaluation of multiplex PCR-dipstick DNA chromatography assay for simultaneous diagnosis of four sexually transmitted pathogens.

J Microbiol Methods 2021 Jul 21;186:106250. Epub 2021 May 21.

Second School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, China; Department of Laboratory Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China. Electronic address:

Introduction: Rapid, sensitive, and specific diagnostic methods are indispensable for sexually transmitted infections (STIs). In this study, a multiplex PCR-dipstick DNA chromatography assay for diagnosis of four STI pathogens, namely Neisseria gonorrhoeae (N. gonorrhoeae), Mycoplasma hominis (M. hominis), Ureaplasma (U. urealyticum and U. parvum), and Chlamydia trachomatis (C. trachomatis), was established and evaluated.

Methods: Based on the hybridization of probes and interaction between streptavidin and biotin, PCR products were visualized through hybridization of specific probes and enzymatic color generation. The sensitivity and specificity of all four pathogens were evaluated. Clinical performance of the test was evaluated using 295 specimens, and comparisons among results were determined via culture or colloidal gold assay.

Results: No cross-reactions were observed, confirming the high specificity of this method. The limit of detection (LOD) of the four STI pathogens was 100 copies/μL. The sensitivity between PCR-dipstick DNA chromatography and culture or colloidal gold assay ranged from 84.6% to 100%. The specificity was between 93.5% and 96.6%, positive predictive value ranged from 53.6% to 86.7%, negative predictive value was over 98.3%, kappa value ranged from 0.676 to 0.864 (Cohen's kappa coefficient test), and the agreement rate was over 93.5%.

Conclusion: In conclusion, PCR-dipstick DNA chromatography serves as a rapid, sensitive, and specific method for simultaneous diagnosis of four STI pathogens.
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http://dx.doi.org/10.1016/j.mimet.2021.106250DOI Listing
July 2021

The efficacy and safety of preoperative cholangiography via percutaneous transhepatic gallbladder drainage (PTGBD) for difficult laparoscopic cholecystectomy (LC).

Surg Endosc 2021 May 19. Epub 2021 May 19.

Department of Hepatobiliary Surgery, General Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, 107 West Wen Hua Road, Jinan, 250012, China.

Background: Percutaneous transhepatic gallbladder drainage (PTGBD) is an important procedure for initial treatment of severe acute cholecystitis (AC) that is contraindicated for early laparoscopic cholecystectomy (LC). We presented our primary experience on a new approach of cholangiography via PTGBD (PTGBD-C) for preoperative delineation of biliary anatomy.

Methods: A retrospective analysis was conducted on 93 patients who received PTGBD followed by LC for AC, with allocation into 2 groups that were PTGBD with (PTGBD-C group, 32 patients) or without (PTGBD-N group, 61 patients) cholangiography. All the clinical data, including demographics, cholangiography findings, operations, and complications, were collected and analyzed.

Results: Cholangiography was attempted in 32 patients with a success of 31 cases, and the most common complication was transient fever in 3 patients. PTGBD-C group of patients showed significantly less operation time (83.2 ± 22.32 vs. 106.5 ± 40.25 min, P = 0.041) and conversion rate (0 vs. 2). There was no statistical difference in terms of postoperative hospitalization and complications.

Conclusions: PTGBD-C is a feasible and safe procedure for severe AC patients with delayed LC. It has advantages of direct cholangiography, being easy to perform and cost-effective, thus should be considered for clinical usage.
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http://dx.doi.org/10.1007/s00464-021-08414-0DOI Listing
May 2021

The Impact of Intraoperative Frozen Section on Resection Margin Status and Survival of Patients Underwent Pancreatoduodenectomy for Distal Cholangiocarcinoma.

Front Oncol 2021 3;11:650585. Epub 2021 May 3.

Department of Hepatobiliary Surgery, General Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Background: Intraoperative frozen section (FS) is broadly used during pancreaticoduodenectomy (PD) to ensure a negative margin status, but its survival benefits on obtaining a secondary R0 resection for distal cholangiocarcinoma (dCCA) is controversial and unclear.

Methods: Clinical data of 107 patients who underwent PD for dCCA was retrospectively collected and divided into different groups based on use of FS (FS and non-FS groups) and status of resection margin (pR0, sR0 and R1 groups), and clinical parameters and survival of patients were compared and analyzed accordingly.

Results: There were 50 patients in FS group with a median survival of 28 months, 57 patients in non-FS group with a median survival of 27 months. There was no statistical difference between the two groups with Kaplan-Meier survival analysis ( = 0.347). There were 98 patients in R0 group (88 in pR0 and 10 in sR0) and nine patients in R1 group, with a median survival of 29 months and 22 months respectively, which showed a better survival in R0 group than in R1 group ( = 0.006). Survival analyses between subgroups revealed difference between pR0 and R1 group ( = 0.005), while no statistical difference concerning pR0 . sR0 ( = 0.211) and sR0 . R1 groups ( = 0.262). Multivariate Cox regression analysis revealed resection margin status, pre-operative biliary drainage and lymph node invasion to be independent prognostic factors for dCCA patients.

Conclusions: Intraoperative FS should be recommended as it significantly increased the rate of R0 resection, which was positively related to a better survival. A primary R0 resection should also be encouraged and if not, a secondary R0 could be considered at the discretion of surgeons as it showed similar survival with primary R0 resection.
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http://dx.doi.org/10.3389/fonc.2021.650585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127005PMC
May 2021

LncRNA GAS5 inhibits miR-579-3p to activate SIRT1/PGC-1α/Nrf2 signaling pathway to reduce cell pyroptosis in sepsis-associated renal injury.

Am J Physiol Cell Physiol 2021 Jul 19;321(1):C117-C133. Epub 2021 May 19.

Department of Critical Care Medicine, Affiliated Chenzhou Hospital, Southern Medical University (The First People's Hospital of Chenzhou), Chenzhou, People's Republic of China.

Sepsis is a life-threatening condition that can lead to several organ failures including kidney. In this study, we investigated the roles of GAS5 and miR-579-3p in regulating cell pyroptosis in the sepsis-induced renal injury model. Lipopolysaccharide (LPS) treatment or cecal ligation and puncture (CLP) surgery was used to create the in vitro and in vivo sepsis-induced renal injury model. The interactions between GAS5 and miR-579-3p, and miR-579-3p and SIRT1 were determined by bioinformatic prediction, luciferase reporter assay, and RIP assay. In vitro cell pyroptosis was examined by flow cytometry marked with active caspase-1 and PI. The protein levels of IL-1β and IL-18 induced by cell pyroptosis were quantified using ELISA assay. In vivo renal injuries were evaluated with HE and TUNEL stainings, bacterial load in serum and creatinine, and blood urea nitrogen content analyses. Expression levels of GAS5, miR-579-3p, pyroptosis, and SIRT1/PGC-1a/Nrf2 pathway-related molecules were evaluated by qRT-PCR or Western blot. GAS5 and SIRT1 were downregulated, whereas miR-579-3p was upregulated in in vitro and in vivo sepsis-induced renal injury models. GAS5 negatively and directly regulated miR-579-3p to reduce cell pyroptosis via the activation of SIRT1/PGC-1a/Nrf2 pathway. In addition, miR-579-3p suppressed PGC-1a/Nrf2 pathway to induce cell pyroptosis by directly targeting SIRT1. What's more, overexpression of GAS5, or knockdown of miR-579-3p, enhanced SIRT1 expression that led to the improved survival rate, reduced the weight loss, and relieved renal injuries in septic mice. Overexpression of GAS5 demonstrated protective effects against sepsis-induced renal injury via downregulating miR-579-3p and activating SIRT1/PGC-1α/Nrf2 pathway to inhibit cell pyroptosis.
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http://dx.doi.org/10.1152/ajpcell.00394.2020DOI Listing
July 2021

Radiological changes on chest CT following COVID-19 infection.

Ann Acad Med Singap 2021 Apr;50(4):346-348

Division of Infectious diseases, Xiangyang No. 1 People's Hospital Affiliated to Hubei University of Medicine, China.

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April 2021

The Efficacy of Moxibustion for Breast Cancer Patients with Chemotherapy-Induced Myelosuppression during Adjuvant Chemotherapy: A Randomized Controlled Study.

Evid Based Complement Alternat Med 2021 25;2021:1347342. Epub 2021 Apr 25.

Department of Breast Surgery, Yueyang Hospital of Integrated Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.

Objective: The randomized controlled clinical trial aims to investigate the clinical efficacy of moxibustion for breast cancer patients with chemotherapy-induced myelosuppression (CIM) during adjuvant chemotherapy.

Methods: Surgically resected breast cancer patients were randomly divided into the moxibustion group (MOX;  = 48) and control group (CON;  = 44). Routine adjuvant chemotherapy (every 21 days, 4-8 cycles) and supportive recombinant human granulocyte colony-stimulating factor were given to both groups, while MOX received an additional moxibustion treatment (once daily after each cycle of chemotherapy). Primary endpoints included the grade of myelosuppression in terms of white blood cell (WBC) and absolute neutrophil count (ANC) and the incidence of myelosuppression-related serious adverse events (SAEs). Other measures included treatment compliance, adverse events (AEs), and survival.

Results: WBC counts were generally higher in MOX and were dramatically higher than those in CON at the 7 course of chemotherapy (=0.008), while grade 1 ANC reduction was dramatically lower than that in CON at the 7course of chemotherapy (=0.006). These effects were particularly significant in patients receiving anthracycline-taxane combination regimens. Moreover, MOX had fewer febrile neutropenia than CON (=0.051). MOX demonstrated a lower incidence of grade 3-4 myelosuppression ( < 0.05). AEs including grade 2-3 severe nausea, various kinds of pains, and vertigo occurred less frequently in MOX ( < 0.05). No difference in survival was observed between the two groups ( > 0.05).

Conclusion: Moxibustion is effective for treating CIM in breast cancer patients during adjuvant chemotherapy, especially for patients receiving high-dose, long-term, and combined chemotherapy regimens. Moxibustion can reduce the incidence of myelosuppression-related SAE and improve the compliance and safety of chemotherapy in breast cancer.
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http://dx.doi.org/10.1155/2021/1347342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093063PMC
April 2021

Sustained bimatoprost release using gold nanoparticles laden contact lenses.

J Biomater Sci Polym Ed 2021 Jun 21:1-17. Epub 2021 Jun 21.

Department of Ophthalmology, The 942 Hospital of Chinese PLA, Yinchuan City, Ningxia Hui Autonomous Region, China.

Contact lenses are ideally suited for sustained ocular drug delivery to bypass the issues associated with eye drop therapy. However, drugs such as bimatoprost loaded by the conventional soaking method show poor drug uptake, high burst release, and altered critical lens properties. In this study, the effect of gold nanoparticles (GNPs) on bimatoprost loading/uptake from the soaking solution and its release kinetics from the lens was investigated. In one method, GNP solutions of varying strength were loaded into the bimatoprost soaking solution (mM-SS batches), and in another method, the GNPs were included in the contact lens matrix during casting (mM-GN-L batches). The GNPs were spherical with average size of 21.1 nm and -20.1 mV zeta potential. The swelling, oxygen permeability, and optical transmittance of the lens were improved compared to those of the lens drug-loaded by the conventional soaking method (SM-L). The mM-GN-L batches showed significant improvement in drug uptake from the soaking solution compared to the SM-L and mM-SS batches. The studies showed relatively low burst and sustained bimatoprost release up to 72 h compared to 24 h with the SM-L batch. The ability to sustain drug release improved proportionally with an increase in the amount of GNPs in the lens. The presence of GNPs lowered protein adherence. The GNP-laden lenses were deemed safe in ocular irritation and histopathology reports (rabbit model). Further, they showed higher drug retention in the rabbit tear fluid compared to the SM-L lens. In conclusion, the presence of GNPs in contact lenses increased drug uptake from the soaking solution, and improved the and release kinetics without affecting the critical properties of the contact lenses for therapeutic application.
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http://dx.doi.org/10.1080/09205063.2021.1927656DOI Listing
June 2021

Multiparametric Magnetic Resonance Imaging-Based Peritumoral Radiomics for Preoperative Prediction of the Presence of Extracapsular Extension With Prostate Cancer.

J Magn Reson Imaging 2021 May 10. Epub 2021 May 10.

Department of Medical Imaging, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China.

Background: Preoperative prediction of extracapsular extension (ECE) of prostate cancer (PCa) is important to guide clinical decision-making and improve patient prognosis.

Purpose: To investigate the value of multiparametric magnetic resonance imaging (mpMRI)-based peritumoral radiomics for preoperative prediction of the presence of ECE.

Study Type: Retrospective.

Population: Two hundred eighty-four patients with PCa from two centers (center 1: 226 patients; center 2: 58 patients). Cases from center 1 were randomly divided into training (158 patients) and internal validation (68 patients) sets. Cases from center 2 were assigned to the external validation set.

Field Strength/sequence: A 3.0 T MRI scanners (three vendors). Sequence: Pelvic T2-weighted turbo/fast spin echo sequence and diffusion weighted echo planar imaging sequence.

Assessment: The peritumoral region (PTR) was obtained by 3-12 mm (half of the tumor length) 3D dilatation of the intratumoral region (ITR). Single-MRI radiomics signatures, mpMRI radiomics signatures, and integrated models, which combined clinical characteristics with the radiomics signatures were built. The discrimination ability was assessed by area under the receiver operating characteristic curve (AUC) in the internal and external validation sets.

Statistical Tests: Fisher's exact test, Mann-Whitney U-test, DeLong test.

Results: The PTR radiomics signatures demonstrated significantly better performance than the corresponding ITR radiomics signatures (AUC: 0.674 vs. 0.554, P < 0.05 on T2-weighted, 0.652 vs. 0.546, P < 0.05 on apparent diffusion coefficient, 0.682 vs. 0.556 on mpMRI in the external validation set). The integrated models combining the PTR radiomics signature with clinical characteristics performed better than corresponding radiomics signatures in the internal validation set (eg. AUC: 0.718 vs. 0.671, P < 0.05 on mpMRI) but performed similar in the external validation set (eg. AUC: 0.684, vs. 0.682, P = 0.45 on mpMRI).

Data Conclusion: The peritumoral radiomics can better predict the presence of ECE preoperatively compared with the intratumoral radiomics and may have better generalization than clinical characteristics. EVIDENCE LEVEL: 4 TECHNICAL EFFICACY: 2.
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http://dx.doi.org/10.1002/jmri.27678DOI Listing
May 2021

Taurine Attenuates the Hypotaurine-Induced Progression of CRC ERK/RSK Signaling.

Front Cell Dev Biol 2021 15;9:631163. Epub 2021 Apr 15.

Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Colorectal cancer (CRC) is one of the most common malignant tumors, and previous metabolomics work has demonstrated great promise in identifying specific small molecules of tumor phenotype. In the present study, we analyzed the metabolites of resected tissues through gas chromatography-mass spectrometry (GC-MS), and found that the concentration of taurine in CRC tissues diminished whereas the concentration of hypotaurine increased. The results demonstrated that taurine significantly suppressed cellular proliferation, metastasis, and colony formation whereas it induced apoptosis in CRC cells. Furthermore, taurine regulated the expression levels of epithelial mesenchymal transition (EMT)-associated genes in a dose-dependent manner. Taurine also alleviated hypotaurine-induced CRC progression, which was linked to the inhibition of the ERK/RSK-signaling pathway and diminution in intracellular hypotaurine. Taurine additionally attenuated hypotaurine-induced tumor growth and metastasis Patients with CRC exhibited lower levels of serum taurine, suggesting that taurine might be a promising biomarker reflecting a poor prognosis in CRC. Collectively, our results demonstrated that taurine-attenuated, hypotaurine-induced CRC progression provides a potential target for CRC therapy.
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http://dx.doi.org/10.3389/fcell.2021.631163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083965PMC
April 2021

A nomogram to predict rupture risk of middle cerebral artery aneurysm.

Neurol Sci 2021 Apr 15. Epub 2021 Apr 15.

Department of Radiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.

Background: Determining the rupture risk of unruptured intracranial aneurysm is crucial for treatment strategy. The purpose of this study was to predict the rupture risk of middle cerebral artery (MCA) aneurysms using a machine learning technique.

Methods: We retrospectively reviewed 403 MCA aneurysms and randomly partitioned them into the training and testing datasets with a ratio of 8:2. A generalized linear model with logit link was developed using training dataset to predict the aneurysm rupture risk based on the clinical variables and morphological features manually measured from computed tomography angiography. To facilitate the clinical application, we further constructed an easy-to-use nomogram based on the developed model.

Results: Ruptured MCA aneurysm had larger aneurysm size, aneurysm height, perpendicular height, aspect ratio, size ratio, bottleneck factor, and height-width ratio. Presence of a daughter-sac was more common in ruptured than in unruptured MCA aneurysms. Six features, including aneurysm multiplicity, lobulations, size ratio, bottleneck factor, height-width ratio, and aneurysm angle, were adopted in the model after feature selection. The model achieved a relatively good performance with areas under the receiver operating characteristic curves of 0.77 in the training dataset and 0.76 in the testing dataset. The nomogram provided a visual interpretation of our model, and the rupture risk probability of MCA aneurysms can be directly read from it.

Conclusion: Our model can be used to predict the rupture risk of MCA aneurysm.
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http://dx.doi.org/10.1007/s10072-021-05255-6DOI Listing
April 2021

Concise Synthesis of Chromene/Chromane-Type Aryne Precursors and Their Applications.

J Org Chem 2021 05 14;86(9):6765-6779. Epub 2021 Apr 14.

Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China.

The gram-scale synthesis of 5,6-, 6,7-, and 7,8-chromene/chromane-type aryne precursors and their applications in regioselective transformation to other functional derivatives is reported. Chromene/chromane-type arynes are generated under mild conditions, which can further undergo [2 + 2], [3 + 2], and [4 + 2] cycloaddition reactions, nucleophilic addition reactions, and σ-insertion reactions to produce structurally novel substituted chromenes and chromanes. The excellent regioselectivity of the reaction is facilitated by the oxygen-containing guiding groups at the -position of the triple bond, which can be removed or switched to other functional groups including alkenyl, aryl, heteroaryl, and arylamino groups.
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http://dx.doi.org/10.1021/acs.joc.1c00493DOI Listing
May 2021

A novel gene variant (c.857A>G; p.Tyr286Cys) in an extended family with non‑syndromic deafness 2A.

Mol Med Rep 2021 06 13;23(6). Epub 2021 Apr 13.

Department of Otolaryngology‑Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi'an, Shaanxi 710032, P.R. China.

Deafness is one of the most common sensory disorders found in humans; notably, >60% of all cases of deafness have been attributed to genetic factors. Variants in potassium voltage‑gated channel subfamily Q member 4 () are etiologically linked to a type of progressive hearing loss, deafness non‑syndromic autosomal dominant 2A (DFNA2A). In the present study, whole‑exome sequencing (WES) was performed on three members of a five‑generation Chinese family with 46 members with hearing loss. Pure tone audiometry and Sanger sequencing were performed for 11 family members to determine whether the novel variant in the gene was segregated with the affected family members. In addition, evolutionary conservation analysis and computational tertiary structure protein prediction of the wild‑type KCNQ4 protein and its variant were performed. The family exhibited autosomal dominant, progressive, post‑lingual, non‑syndromic sensorineural hearing loss. A novel co‑segregating heterozygous missense variant (c.857A>G; p.Tyr286Cys) in the glycine‑tyrosine‑glycine signature sequence in the pore region of the KCNQ4 channel was identified. This variant was predicted to result in a tyrosine‑to‑cysteine substitution at position 286 in the KCNQ4 protein. The tyrosine at position 286 is well conserved across different species. The substitution of tyrosine with cysteine would affect the structure of the pore region, resulting in the loss of channel function. The gene is one of the most common mutated genes observed in patients with autosomal dominant, non‑syndromic hearing loss. Taken together, for the family analyzed in the present study, performing WES in conjunction with Sanger sequencing has led to the detection of a novel, potentially causative variant (c.857 A>G; p.Tyr286Cys) in exon 6 of the gene. The present study has added to the number of pathogenic variants observed in the KCNQ4 gene, and the findings may prove to be useful for both the diagnosis of DFNA2A and in the design of early interventional therapies.
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http://dx.doi.org/10.3892/mmr.2021.12059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025472PMC
June 2021

Changes in the Leaf Physiological Characteristics and Tissue-Specific Distribution of Ginsenosides in During Flowering Stage Under Cold Stress.

Front Bioeng Biotechnol 2021 17;9:637324. Epub 2021 Mar 17.

Key Laboratory of Chinese Medicine Planting and Development, Changchun University of Chinese Medicine, Changchun, China.

is a valuable traditional herbal medicine material with numerous applications. Ginsenosides are the key bioactive compounds in ginseng. Cold stress can activate stress tolerance mechanisms that regulate biomass and biosynthesis in ginseng tissue. In this study, the effects of short- and long-term cold stress (5°C) on the physiological characteristics, tissue-specific ginsenoside distributions, and ginsenoside synthesis gene expressions of 3-year-old during the flowering period were investigated. Short-term cold stress significantly reduced ginseng biomass (root fresh weight and dry weight), and increased malondialdehyde, proline, soluble sugar, and soluble protein concentrations. Superoxide dismutase, peroxidase, and catalase activities also increased significantly under cold stress. With prolongation of the cold stress period, all antioxidant enzyme activity decreased. The protopanaxatriol-type ginsenoside concentrations in the taproots (phloem and xylem) and fibrous roots, as well as the protopanaxadiol-type ginsenoside concentrations in the leaves, increased significantly under short-term cold stress. The key genes (, , , and ) involved in the ginsenoside biosynthesis pathway were significantly positively correlated with the ginsenoside accumulation trends. Thus, short-term cold stress can stimulate membrane lipid peroxidation, in turn stimulating the antioxidant enzyme system to alleviate oxidative damage and increasing the expression of key enzyme genes involved in ginsenoside biosynthesis. During agricultural production, protopanaxadiol/protopanaxatriol ratios could be manipulated by low-temperature storage or treatments.
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http://dx.doi.org/10.3389/fbioe.2021.637324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011539PMC
March 2021

[Influence of canine vertical position on smile esthetic perceptions by using 3D digital facial reconstruction].

Shanghai Kou Qiang Yi Xue 2020 Dec;29(6):611-616

School and Hospital of Stomatology, Tongji University. Shanghai 200072, China.

Purpose: To verify the impact of alterations in the vertical position of maxillary canine on smile esthetic perceptions, in order to find an optimal canine position.

Methods: A volunteer's dentition and facial images were scanned by intra-oral scanning and three-dimensional facial scanning, which were then calibrated. In virtue of dental computer-aided design software, the canine vertical positions were symmetrically modified in a variable of 0.5 mm, creating 4 new 3D virtual digital smile faces varying from 1.0 mm of intrusion to 1.0 mm of extrusion. A total of 5 3D virtual digital smile faces were evaluated from the front and 45°to the right sides by 60 dentists and 60 laypersons on a visual analog scale. The data were input into SPSS 20.0 software package, ANOVA of repeated measurement was used to compare the differences in the assessment scores between two groups and rank sum test was used to analyze the differences from different evaluation angles.

Results: Alterations in the vertical position of maxillary canine had significant influence on the esthetic evaluation of the smile(P<0.05); there was significant difference between the dentists' and laypersons' esthetic evaluations after the canine vertical position being modified(P<0.05), there was no significant difference on the evaluations of smile esthetics from different evaluation perspectives(P>0.05).

Conclusions: The most attractive smile was the one with canine cusp 0.5 mm vertically higher than the incisal edge of the lateral incisor. The smile is not attractive when the cusp of canine is vertically lower than the incisal edge of lateral incisor. These results can be used as references in the esthetic treatment planning for anterior maxillary teeth.
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December 2020
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