Publications by authors named "Qingzhi Wang"

58 Publications

Persistent mTORC1 activation via Depdc5 deletion results in spontaneous hepatocellular carcinoma but does not exacerbate carcinogen- and high-fat diet-induced hepatic carcinogenesis in mice.

Biochem Biophys Res Commun 2021 Nov 20;578:142-149. Epub 2021 Sep 20.

School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang Medical University, Xinxiang, Henan, China. Electronic address:

The mechanistic target of rapamycin complex 1 (mTORC1) acts as a central regulator of metabolic pathways that drive cellular growth. Abnormal activation of mTORC1 occurs at high frequency in human and mouse hepatocellular carcinoma (HCC). DEP domain-containing protein 5 (DEPDC5), a component of GATOR1 complex, is a repressor of amino acid-sensing branch of the mTORC1 pathway. In the current study, we found that persistent activation of hepatic mTORC1 signaling caused by Depdc5 ablation was sufficient to induce a pathological program of liver damage, inflammation and fibrosis that triggers spontaneous HCC development. Take advantage of the combinatory treatment with a single dose of diethylnitrosamine (DEN) and chronic feeding with high-fat diet (HFD), we demonstrated that hepatic depdc5 deletion did not aggravate DEN&HFD induced liver tumorigenesis, probably due to its protective effects on diet-induced liver steatosis. In addition, we further showed that chronic rapamycin treatment did not have any apparent tumor-suppressing effects on DEN&HFD treated control mice, whereas it dramatically reduced the tumor burden in mice with hepatic Depdc5 ablation. This study provides the novel in vivo evidence for Depdc5 deletion mediated mTORC1 hyperactivation in liver tumorigenesis caused by aging or DEN&HFD treatment. Moreover, our findings also propose that pharmacological inhibition of mTORC1 signaling maybe a promising strategy to treat HCC patients with mutations in DEPDC5 gene.
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http://dx.doi.org/10.1016/j.bbrc.2021.09.036DOI Listing
November 2021

Glycerol or crude glycerol as substrates make Pseudomonas aeruginosa achieve anaerobic production of rhamnolipids.

Microb Cell Fact 2021 Sep 23;20(1):185. Epub 2021 Sep 23.

Research Institute of Petroleum Exploration and Development (Langfang), Langfang, 065007, Hebei, China.

Background: The anaerobic production of rhamnolipids is significant in research and application, such as foamless fermentation and in situ production of rhamnolipids in the anoxic environments. Although a few studies reported that some rare Pseudomonas aeruginosa strains can produce rhamnolipids anaerobically, the decisive factors for anaerobic production of rhamnolipids were unknown.

Results: Two possible hypotheses on the decisive factors for anaerobic production of rhamnolipids by P. aeruginosa were proposed, the strains specificity of rare P. aeruginosa (hypothesis 1) and the effect of specific substrates (hypothesis 2). This study assessed the anaerobic growth and rhamnolipids synthesis of three P. aeruginosa strains using different substrates. P. aeruginosa strains anaerobically grew well using all the tested substrates, but glycerol was the only carbon source that supported anaerobic production of rhamnolipids. Other carbon sources with different concentrations still failed for anaerobic production of rhamnolipids by P. aeruginosa. Nitrate was the excellent nitrogen source for anaerobic production of rhamnolipids. FTIR spectra analysis confirmed the anaerobically produced rhamnolipids by P. aeruginosa using glycerol. The anaerobically produced rhamnolipids decreased air-water surface tension to below 29.0 mN/m and emulsified crude oil with EI above 65%. Crude glycerol and 1, 2-propylene glycol also supported the anaerobic production of rhamnolipids by all P. aeruginosa strains. Prospects and bottlenecks to anaerobic production of rhamnolipids were also discussed.

Conclusions: Glycerol substrate was the decisive factor for anaerobic production of rhamnolipids by P. aeruginosa. Strain specificity resulted in the different anaerobic yield of rhamnolipids. Crude glycerol was one low cost substrate for anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Results help advance the research on anaerobic production of rhamnolipids, deepen the biosynthesis theory of rhamnolipids and optimize the anaerobic production of rhamnolipids.
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http://dx.doi.org/10.1186/s12934-021-01676-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461908PMC
September 2021

Finite-time stabilization and H∞ control of Port-controlled Hamiltonian systems with disturbances and saturation.

PLoS One 2021 16;16(8):e0255797. Epub 2021 Aug 16.

College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, Shandong Province, China.

The finite-time stabilization and finite-time H∞ control problems of Port-controlled Hamiltonian (PCH) systems with disturbances and input saturation (IS) are studied in this paper. First, by designing an appropriate output feedback, a strictly dissipative PCH system is obtained and finite-time stabilization result for nominal system is given. Second, with the help of the Hamilton function method and truncation inequality technique, a novel output feedback controller is developed to make the PCH system finite-time stable when IS occurs. Further, a finite-time H∞ controller is designed to attenuate disturbances for PCH systems with IS, and sufficient conditions are presented. Finally, a numerical example and a circuit example are given to reveal the feasibility of the obtained theoretical results.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0255797PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366989PMC
August 2021

Consultation length, process quality and diagnosis quality of primary care in rural China: A cross-sectional standardized patient study.

Patient Educ Couns 2021 Aug 8. Epub 2021 Aug 8.

Department of Health Behavior and Social Medicine, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China. Electronic address:

Objective: Consultation length, the time spent between patient and health care provider during a visit, is an essential element in measuring quality of health care patients receive from a primary care facility. However, the linkage between consultation length and process quality and diagnosis quality of primary care is still uncertain. This study aims to examine the role consultation length plays in delivering process quality and diagnosis quality, two central components of overall primary care quality, in rural China.

Methods: We recruited unannounced standardized patients (SPs) to present classic symptoms of angina and tuberculosis in selected healthcare facilities in three provinces of China. The consultation length and primary care quality of SPs were measured and compared with both international and national standards of care. Ordinary Least Squares (OLS) regressions for process quality (continuous dependent variable) and Logistic regressions for diagnosis quality (binary dependent variable) were performed to investigate the relationship between consultation length and primary care quality.

Results: The average consultation lengths among patients with classic symptoms of angina and those with symptoms of tuberculosis were approximately 4.33 min and 6.28 min, respectively. Providers who spent more time with patients were significantly more likely to complete higher percentage of recommended checklist items of both questions and examinations for angina (β = 1.39, 95%CI 1.01-1.78) and tuberculosis (β = 0.89, 95%CI 0.69-1.08). Further, providers who spent more time with patients were more likely to make correct diagnosis for angina (marginal effect = 0.014, 95%CI 0.002-0.026) and for tuberculosis (marginal effect = 0.013, 95%CI 0.005-0.021).

Conclusions: The average consultation length is extremely short among primary care providers in rural China. The longer consultation leads to both better process and diagnosis quality of primary care.

Practice Implications: We recommend primary care providers to increase the length of their communication with patients. To do so, government should implement healthcare reforms to clarify the requirements of affordable and reliable consultation length in medical care services. Moreover, such an experience can also be extended to other developing countries.
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http://dx.doi.org/10.1016/j.pec.2021.08.006DOI Listing
August 2021

Foraging behavior of the sea urchin Mesocentrotus nudus exposed to conspecific alarm cues in various conditions.

Sci Rep 2021 08 2;11(1):15654. Epub 2021 Aug 2.

Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China.

Conspecific alarm cues crushed from Mesocentrotus nudus prevent sea urchins from foraging the kelp, but do not repel them far away from the kelp. However, it remains largely unknown of whether this phenomenon was affected by conspecific alarm cues or by the attraction of the kelp. The present study found no significant difference in the duration in the danger area with or without the kelp around conspecific alarm cues. This suggests that the phenomenon is the strategy of sea urchins but not by the attraction of kelp. We found that conspecific alarm cues appearing between the kelp and sea urchins significantly affected foraging behavior of sea urchins fasted for 21 days. This indicates that conspecific alarm cues can effectively prevent fasted sea urchins from foraging the kelp. Further, there was no correlation between foraging velocity and the duration in the danger area. Pearson correlation analysis revealed no significant correlation between foraging velocity and the duration in the safety area close to different amounts of conspecific alarm cues, suggesting that conspecific alarm cues prevent sea urchins with strong foraging ability to forage. Collectively, the present results indicate that conspecific alarm cues as highly available biological barriers are cost-effective approaches to preventing overgrazing of sea urchins in the protection of kelp beds ecosystems. Notably, the present study is a short-term laboratory investigation that does not consider the complexity of natural conditions. Future studies are essential to test the present findings in the field.
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http://dx.doi.org/10.1038/s41598-021-94969-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329164PMC
August 2021

Adipocyte-specific deletion of Depdc5 exacerbates insulin resistance and adipose tissue inflammation in mice.

Biochem Biophys Res Commun 2021 Sep 7;569:118-124. Epub 2021 Jul 7.

School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China. Electronic address:

The mammalian target of rapamycin complex 1 (mTORC1) is a crucial regulator of adipogenesis and systemic energy metabolism. Its dysregulation leads to a diversity of metabolic diseases, including obesity and type 2 diabetes. DEP-domain containing 5 (DEPDC5) is a critical component of GATOR1 complex that functions as a key inhibitor of mTORC1. So far, its function in adipose tissue remains largely unknown. Herein we evaluated how persistent mTORC1 activation in adipocyte via Depdc5 knockout modulates adiposity in vivo. Our data indicated that adipocyte-specific knockout of Depdc5 in aged mice led to reduced visceral fat, aggravated insulin resistance and enhanced adipose tissue inflammation. Moreover, we found that Depdc5 ablation resulted in upregulation of adipose triglyceride lipase (ATGL) in adipocytes and elevated levels of serum free fatty acids (FFAs). Intriguingly, rapamycin treatment did not reverse insulin resistance but alleviated adipose tissue inflammation caused by Depdc5 deletion. Taken together, our findings revealed that mTORC1 activation caused by Depdc5 deletion promotes lipolysis process and further exacerbates insulin resistance and adipose tissue inflammation in mice.
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http://dx.doi.org/10.1016/j.bbrc.2021.07.003DOI Listing
September 2021

Chromosome-level genome assembly of Scapharca kagoshimensis reveals the expanded molecular basis of heme biosynthesis in ark shells.

Mol Ecol Resour 2021 Jul 2. Epub 2021 Jul 2.

Liaoning Ocean and Fisheries Science Research Institute and Dalian Key Laboratory of Genetic Resources for Marine Shellfish, Dalian, China.

Ark shells are commercially important clam species that inhabit in muddy sediments of shallow coasts in East Asia. For a long time, the lack of genome resources has hindered scientific research of ark shells. Here, we report a high-quality chromosome-level genome assembly of Scapharca kagoshimensis, with an aim to unravel the molecular basis of heme biosynthesis, and develop genomic resources for genetic breeding and population genetics in ark shells. Nineteen scaffolds corresponding to 19 chromosomes were constructed from 938 contigs (contig N50 = 2.01 Mb) to produce a final high-quality assembly with a total length of 1.11 Gb and scaffold N50 around 60.64 Mb. The genome assembly represents 93.4% completeness via matching 303 eukaryota core conserved genes. A total of 24,908 protein-coding genes were predicted and 24,551 genes (98.56%) of which were functionally annotated. The enrichment analyses suggested that genes in heme biosynthesis pathways were expanded and positive selection of the haemoglobin genes was also found in the genome of S. kagoshimensis, which gives important insights into the molecular mechanisms and evolution of the heme biosynthesis in mollusca. The valuable genome assembly of S. kagoshimensis would provide a solid foundation for investigating the molecular mechanisms that underlie the diverse biological functions and evolutionary adaptations of S. kagoshimensis.
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http://dx.doi.org/10.1111/1755-0998.13460DOI Listing
July 2021

Characterization of the complete mitochondrial genome of (Bivalvia: Ostreidae).

Mitochondrial DNA B Resour 2021 May 4;6(5):1581-1582. Epub 2021 May 4.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China.

In this study, we sequenced and analyzed the complete mitochondrial genome of (Gmelin 1791), the newly determined mitochondrial genome is 18225 bp in length, it is a circular molecule and consists of 12 protein-coding genes ( is absent), 24 transfer RNA (with two copies of and ), and 2 ribosomal RNA genes (splitting of the gene and duplication of the gene were identified). Phylogenetic analysis based on 12 protein coding genes showed that is closely related to .
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http://dx.doi.org/10.1080/23802359.2021.1915205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218865PMC
May 2021

Anaerobic biosynthesis of rhamnolipids by Pseudomonas aeruginosa: performance, mechanism and its application potential for enhanced oil recovery.

Microb Cell Fact 2021 May 20;20(1):103. Epub 2021 May 20.

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, Liaoning Province, China.

Background: Pseudomonas aeruginosa, the rhamnolipids-producer, is one of dominant bacteria in oil reservoirs. Although P. aeruginosa strains are facultative bacteria, the anaerobic biosynthesis mechanism of rhamnolipids is unclear. Considering the oxygen scarcity within oil reservoirs, revealing the anaerobic biosynthesis mechanism of rhamnolipids are significant for improving the in-situ production of rhamnolipids in oil reservoirs to enhance oil recovery.

Results: Pseudomonas aeruginosa SG anaerobically produced rhamnolipids using glycerol rather than glucose as carbon sources. Two possible hypotheses on anaerobic biosynthesis of rhamnolipids were proposed, the new anaerobic biosynthetic pathway (hypothesis 1) and the highly anaerobic expression of key genes (hypothesis 2). Knockout strain SGΔrmlB failed to anaerobically produce rhamnolipids using glycerol. Comparative transcriptomics analysis results revealed that glucose inhibited the anaerobic expression of genes rmlBDAC, fabABG, rhlABRI, rhlC and lasI. Using glycerol as carbon source, the anaerobic expression of key genes in P. aeruginosa SG was significantly up-regulated. The anaerobic biosynthetic pathway of rhamnolipids in P. aeruginosa SG were confirmed, involving the gluconeogenesis from glycerol, the biosynthesis of dTDP-L-rhamnose and β-hydroxy fatty acids, and the rhamnosyl transfer process. The engineered strain P. aeruginosa PrhlAB constructed in previous work enhanced 9.67% of oil recovery higher than the wild-type strain P. aeruginosa SG enhancing 8.33% of oil recovery.

Conclusion: The highly anaerobic expression of key genes enables P. aeruginosa SG to anaerobically biosynthesize rhamnolipids. The genes, rmlBDAC, fabABG, rhlABRI, rhlC and lasI, are key genes for anaerobic biosynthesis of rhamnolipid by P. aeruginosa. Improving the anaerobic production of rhamnolipids better enhanced oil recovery in core flooding test. This study fills the gaps in the anaerobic biosynthesis mechanism of rhamnolipids. Results are significant for the metabolic engineering of P. aeruginosa to enhance anaerobic production of rhamnolipids.
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http://dx.doi.org/10.1186/s12934-021-01593-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139158PMC
May 2021

[Status and influencing factors of breastfeeding behavior in rural areas of Han, Tibetan and Yi nationalities in Sichuan Province].

Wei Sheng Yan Jiu 2021 Mar;50(2):250-255

Department of Health Related Social and Behavioral Science, West China School of Public Health, Sichuan University, Chengdu 610041, China.

Objective: To evaluate the status of breastfeeding among infants aged 0-18 months in rural areas of Han, Tibetan and Yi ethnic in Sichuan Province, and to explore the relevant impact factors.

Methods: Using multistage sampling method, choose two Han county, two Tibetan county and two Yi county rural 0-18 months infants and young children with their mother as the subjects in Sichuan Province, to collect mothers and infants and young children's demography, family status, the information such as breastfeeding knowledge and behavior through questionnaires. Unconditional Logistic regression was used to analyze the influencing factors of breastfeeding.

Results: Among the 1087 infants, early initiation of breastfeeding rate was 22. 45%, among the 985 infants aged 6 months and above, the rate of exclusive breastfeeding under 6 months was 35. 94%, among the 658 infants aged 12 months and above, the rate of continued breastfeeding at 1 year was 34. 50%. The favorable factors for early initiation of breastfeeding were that normal delivery(OR=2. 80, 95% CI 1. 82-4. 30) and mothers with knowledge of early initiation of breastfeeding behavior(OR=2. 48, 95% CI 1. 69-3. 64). Tibetan mother(OR=1. 56, 95% CI 1. 02-2. 39), very good family economic status(OR=2. 70, 95% CI 1. 47-4. 98), mother's knowledge of exclusive breastfeeding under 6 months(OR=1. 47, 95% CI 1. 07-2. 02) were favorable factors for exclusive breastfeeding behavior under 6 months. Second birth and above(OR=1. 67, 95% CI 1. 11-2. 52) and mothers' knowledge of weaning(OR=3. 96, 95% CI 2. 65-5. 93) were favorable factors for continued breastfeeding at 1 year.

Conclusion: The status of breastfeeding in the multi-ethnic rural areas of Sichuan Province is poor. The mother's ethnicity, delivery method, and whether they have relevant knowledge, parity and family economic status will affect the breastfeeding behavior of infant mothers.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.02.014DOI Listing
March 2021

Characterization of the complete mitochondrial genome of (Littorinimorpha: Ficidae) and molecular phylogeny of Caenogastropoda.

Mitochondrial DNA B Resour 2021 Mar 19;6(3):1126-1128. Epub 2021 Mar 19.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, PR China.

Ficidae is a family of chiefly tropical marine gastropod mollusks with approximately 20 described species. Hitherto, there are no complete mitochondrial genome (mitogenome) of Ficidae available for the Ficoidea. Here, we determined the complete mitogenome of Röding, 1798 representing the first species from the family Ficidae. The newly sequenced mitogenome consists of 13 protein-coding genes, two ribosomal genes, and 22 transfer genes. All of 13 PCGs use ATG as initiation codons and end with conventional stop codons TAA and TAG, and the genome organization is similar to those of other documented caenogastropod mitogenomes. Tonnoidea and Ficoidea were recovered as sister group in the Caenogastropoda tree.
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http://dx.doi.org/10.1080/23802359.2021.1901628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995913PMC
March 2021

Alarmingly high malnutrition in childhood and its associated factors: A study among children under 5 in Yemen.

Medicine (Baltimore) 2021 Feb;100(5):e24419

West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.

Abstract: Childhood malnutrition is a serious public health problem in Yemen. However, there is a limited information regarding association of malnutrition with different socio-economic factors. This study examines the correlates of socioeconomic and maternal behavioral factors on malnutrition in Yemeni children under 5 years of age.Our study focuses on the nutritional status of children under 5 years of age, and uses the data provided by the cross-sectional study namely Yemen National Demographic and Health Survey. Three anthropometric indicators: stunting, wasting, and underweight were selected for the evaluation of malnutrition. Independent variables include personal and maternal characteristics, socioeconomic and behavioral factors, and illness conditions. The study used the Chi-Squared test to test the significant association between independent variables and logistic regression to estimate the odds of being malnourished.A total of 13,624 Yemeni children under 5 years of age were included in the study. The results show the high malnutrition level - the prevalence of stunting was 47%, wasting was 16%, and underweight was 39%. There is a statistically significant association between socioeconomic status, behavioral factors, and child malnutrition. The odds of malnutrition decreased with the increase in the level of mother's education, economic status, and frequency of prenatal visits. The odds of malnutrition were least for children whose mothers had highest level of education (OR = 0.64; 95%CI = 0.55-0.76), who belonged to highest wealth index (OR = 0.41; 95%CI = 0.36-0.47). Moreover, the likelihood of malnutrition was less among the children whose mother had highest number of prenatal visits during the pregnancy (OR = 0.67; 95%CI = 0.59-0.76).The high prevalence of stunting, wasting, and undernutrition were found in Yemeni children. Different factors such as regional variations, socio-economic disparities, and maternal education and health care utilization behavior are found to be associated with high malnutrition. These findings provide important policy implications to improving childhood malnutrition in Yemen.
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http://dx.doi.org/10.1097/MD.0000000000024419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870187PMC
February 2021

Sirtuin 6 ameliorates alcohol-induced liver injury by reducing endoplasmic reticulum stress in mice.

Biochem Biophys Res Commun 2021 03 28;544:44-51. Epub 2021 Jan 28.

School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang Medical University, Xinxiang, Henan, China. Electronic address:

Alcoholic liver disease (ALD) occurs as a result of chronic and excessive alcohol consumption. It encompasses a wide spectrum of chronic liver abnormalities that range from steatosis to alcoholic hepatitis, progressive fibrosis and cirrhosis. Endoplasmic reticulum (ER) stress induced by ethanol metabolism in hepatocytes has been established as an important contributor to the pathogenesis of ALD. However, whether SIRT6 exerts regulatory effects on ethanol-induced ER stress and contributes to the pathogenesis of ALD is unclear. In this study, we developed and characterized Sirt6 hepatocyte-specific knockout and transgenic mouse models that were treated with chronic-plus-binge ethanol feeding. We observed that hepatic Sirt6 deficiency led to exacerbated ethanol-induced liver injury and aggravated hepatic ER stress. Tauroursodeoxycholic acid (TUDCA) treatment remarkably attenuated ethanol-induced ER stress and ameliorated ALD pathologies caused by Sirt6 ablation. Reciprocally, SIRT6 hepatocyte-specific transgenic mice exhibited reduced ER stress and ameliorated liver injury caused by ethanol exposure. Consistently, knockdown of Sirt6 elevated the expression of ER stress related genes in primary hepatocytes treated with ethanol, whereas overexpression of SIRT6 reduced their expression, indicating SIRT6 regulates ethanol-induced hepatic ER stress in a cell autonomous manner. Collectively, our results suggest that SIRT6 is a positive regulator of ethanol-induced ER stress in the liver and protects against ALD by relieving ER stress.
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http://dx.doi.org/10.1016/j.bbrc.2021.01.061DOI Listing
March 2021

Can Nepal achieve nutritional targets by 2030? A trend analysis of childhood undernutrition in Nepal from 2001 to 2016.

Public Health Nutr 2021 Jan 21:1-10. Epub 2021 Jan 21.

Department of Health and Social Behavior Science, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu610041, People's Republic of China.

Objective: This study assesses the prevalence of childhood undernutrition from 2001 to 2016 and estimate projections of undernutrition for 2016-2030 in Nepal.

Design: The study used data from four rounds of a cross-sectional survey of Nepal Demographic and Health Survey (NDHS) conducted in 2001, 2006, 2011 and 2016. Descriptive analyses were conducted to calculate prevalence, binary logistic regression was used to test the significance of trends over time and autoregressive integrated moving average model was used to forecast the prevalence of childhood undernutrition.

Settings: The children and household member datasets from four NDHS were merged to assess the trends of childhood undernutrition in Nepal.

Participants: A total of 16 613 children (8399 male and 8214 female) under 5 years of age were selected for anthropometric measurements using a stratified cluster random sampling method.

Results: Overall results show a decline in prevalence of stunting from 57·2 % to 35·8 % (P < 0·001), underweight from 42·7 % to 27 % (P < 0·001) and wasting from 11·2 % to 9·7 % (P < 0·05) from 2001 to 2016. However, different population subgroups have a higher prevalence of undernutrition than national average. Further, the analyses show that the prevalence of stunting will decline to 14·3 % and wasting to 8·4 % by 2030.

Conclusion: A remarkable decrease in the prevalence of stunting and underweight has been observed over the last 15 years. Nepal is likely to achieve the nutritional targets for stunting but not for wasting by 2030. Given large subpopulation variations, further improvement in undernutrition require more specific, targeted and localised programmes.
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http://dx.doi.org/10.1017/S1368980021000240DOI Listing
January 2021

Sirtuin 1 inhibitor EX527 suppresses morphine-induced behavioral sensitization.

Neurosci Lett 2021 01 4;744:135599. Epub 2021 Jan 4.

Xinxiang Key Laboratory of Forensic Toxicology, School of Forensic Medicine, Xinxiang Medical University, Xinxiang, 453003, Henan, China. Electronic address:

Morphine addiction is categorized as a chronic recurrent brain disease which always results in mental disturbance, concomitant diseases and early death. Recent evidence suggested that Sirtuin 1 (SIRT1) played a crucial role in learning, memory and reward, nevertheless, its role in morphine addiction is still unclear. We explored whether SIRT1 in the ventrolateral orbital cortex (VLO) is associated with morphine addiction and its potential mechanism. We applied the morphine-induced behavioral sensitization paradigm to investigate whether microinjection of EX527, a SIRT1 inhibitor, into the VLO could affect the rat behaviors. Furthermore, we focused on the expression of extracellular signal-regulated protein kinases (ERK) and brain-derived neurotrophic factor (BDNF), potential downstream targets of SIRT1. Microinjecting EX527 into the VLO significantly suppressed morphine-induced behavioral sensitization. We found that the expression of SIRT1, phosphorylated ERK (p-ERK) and BDNF in the VLO were markedly up-regulated by morphine administrations in expression phase. These positive changes were significantly inhibited by microinjecting EX527 into the VLO. These results suggest that SIRT1 in the VLO may mediate morphine-induced behavioral sensitization and the overexpression of SIRT1, p-ERK and BDNF could be the potential mechanism. Taken together, the results of our research provide evidence to support that SIRT1 play an important role in morphine vulnerability and microinjecting EX527 into the VLO could significantly suppress morphine addiction in rats.
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http://dx.doi.org/10.1016/j.neulet.2020.135599DOI Listing
January 2021

Organophosphate esters (OPEs) in wetland soil and Suaeda salsa from intertidal Laizhou Bay, North China: Levels, distribution, and soil-plant transfer model.

Sci Total Environ 2021 Apr 14;764:142891. Epub 2020 Oct 14.

Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.

Wetlands have attracted much attention due to releases of organophosphate esters (OPEs) and other emerging contaminants into this particular environment. Here, Suaeda salsa plants and wetland soils collected from Laizhou Bay, North China, were analyzed to investigate the levels, distribution, and soil-plant transfer of OPEs in these ecosystems. The ΣOPEs concentrations ranged from 137 to 386 ng/g dry weight (dw), whereas in rhizosphere the concentrations were between 99.8 and 198 ng/g dw. Suaeda salsa rhizosphere could promote the absorption of OPEs in wetlands, and Suaeda salsa root presents a greater rate of absorption. The ΣOPEs concentrations ranged from 32.9 to 56.8 ng/g dw in roots, 3.93 to 7.51 ng/g dw in stems, and 2.79 to 4.06 ng/g dw in leaves. Log RCFs, log TF and log TF showed no significant correlations with their log K, indicating the complexity of uptake and translocation in the natural environment. Predictive model for the OPEs availability to Suaeda salsa was established from the experimental data. The field-based BCFs of all OPEs were dependent on K, decreased with increasing K. This study provides important insights into the phytoremediation potential of OPEs using Suaeda salsa as an effective strategy and their role in environmental risk assessment of OPEs in wetlands.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142891DOI Listing
April 2021

Tissue distribution and bioaccumulation of organophosphate esters in wild marine fish from Laizhou Bay, North China: Implications of human exposure via fish consumption.

J Hazard Mater 2021 01 6;401:123410. Epub 2020 Jul 6.

Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.

Despite organophosphate esters (OPEs) are ubiquitous in the environment, limited information is available about their tissue-specific accumulation potential in marine fish and exposure risks. Ten fish species from the coastal area of Laizhou Bay, North China, were sampled and analyzed to investigate tissue levels, bioaccumulation, and human exposure risks of 20 OPEs. Seventeen OPEs were detected in fish tissues with total concentration ranging from 6.6-107 ng/g dry weight. The average log bioaccumulation factor (BAF) values of OPEs ranged from 2.8 to 4.4 in livers, 2.3-3.8 in muscles, 2.5-3.9 in gills, and 2.8-4.4 in kidneys. The log BAF values of OPEs significantly increased with increasing their log K values (r = 0.55-0.63, p < 0.001). The estimated daily intake of OPEs ranged from 1.7-12.0 and 3.1-22.1 ng/kg bw/d for rural and urban residents, respectively. The hazard quotients of OPEs were in the range of 4 × 10 to 6.7 × 10 and 7 × 10 to 1.2 × 10 for rural and urban residents, respectively. Results showed that the human health risks of OPEs associated with fish consumption is at low level.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123410DOI Listing
January 2021

A reactive probe for Co ion detection based on a catalytic decomposition process and its fluorescence imaging in living cells.

Luminescence 2021 Feb 15;36(1):4-10. Epub 2020 Jul 15.

Xinxiang Key Laboratory of Forensic Science Evidence, School of Forensic Medicine, Xinxiang Medical University, Jinsui Road No. 601, Xinxiang, Henan Province, China.

A novel reactive fluorescent probe for cobalt ions was prepared based on integration of thiourea functional groups, coumarin, and naphthalimide fluorophores. There was no fluorescence observed for the probe itself, however, in the presence of cobalt ions, catalytic decomposition occurred for the probe and coumarin molecular fragments were produced that emitted blue fluorescence. This enabled the probe to be used as a 'turn on' reagent for detection of cobalt ions. Under physiological pH conditions and in appropriate solvent systems, an obvious fluorescence enhancement for cobalt ions was observed in selective experiments. Competition experiments indicated that cobalt ions could still induce fluorescence enhancement in the presence of other metal ions. Sensitivity experiments showed that the detection limit for cobalt ions was 6.0 nM. Dynamics research demonstrated that the catalytic process was a pseudo-first-order reaction and the reaction constant (k ) was calculated to be 1.49 × 10 min . In addition, the mechanism of catalytic decomposition could be demonstrated using electrospray ionization mass spectrometry and thin layer chromatography experiments. Cell fluorescence imaging experiments demonstrated that the probe could be used to detect cobalt ions in living HeLa cells.
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http://dx.doi.org/10.1002/bio.3909DOI Listing
February 2021

Genetic diversity and population structure of in China revealed by sequence-related amplified polymorphism markers.

PeerJ 2020 25;8:e8723. Epub 2020 Mar 25.

College of Fisheries and Life Science, Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian Ocean University, Dalian, China.

Genetic variation in nine stocks of collected from China was analyzed using sequence-related amplified polymorphism (SRAP) markers. Eight primer pairs produced 132 polymorphic loci with an average of 16.5 loci per primer pair. A population from Jiangsu had the highest percentage of polymorphic loci at 27.27%, suggesting that these resources had a rich genetic diversity. The Nei's gene diversity of the nine populations ranged from 0.0647 to 0.0793; a population from Shandong was the lowest and a population from North Korea the highest. The Shannon's information index was between 0.1023 and 0.1202, with the lowest in the Shandong population and the highest in the Jiangsu population. The Nei's unbiased genetic distance between the nine populations was 0.0243-0.0570 and the genetic similarity was 0.9446-0.9760; the genetic distance between Guangxi and Shandong populations was the furthest (0.0570) and the genetic distance between Shandong and Jiangsu populations was the closest (0.0243). Nei's gene diversity analysis indicated that the genetic variance was mainly found within individual geographical populations, and the analysis of molecular variance revealed low but significant genetic differentiation among local and regional populations. The limited gene flow (Nm = 0.555) was inferred as a major reason for the extent of genetic differentiation in . The results obtained here indicated that have high degree of genetic diversity and the potential of further breeding with excellent germplasm resources. This study provides a scientific basis for the protection of germplasm resources and the breeding of .
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http://dx.doi.org/10.7717/peerj.8723DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102500PMC
March 2020

miR-205-3p Functions as a Tumor Suppressor in Ovarian Carcinoma.

Reprod Sci 2020 01 6;27(1):380-388. Epub 2020 Jan 6.

Heze Municipal Hospital, No. 28888 Caozhou Road, Heze, 274000, Shandong, China.

Ovarian cancer (OC) represents the most lethal form of gynaecologic cancers in developed countries. To develop a better therapeutic against OC, characterizing new classes of molecular regulators such as microRNAs (miRNAs) involved in OC tumorigenesis becomes immensely important. We used human OC cell lines to study the expression pattern of miRNA-205-3p. We then employed miRNA-205-3p mimic and inhibitor to elucidate its functional role in OC cells. Downstream target of miRNA-205-3p was characterized in OC cells with luciferase gene reporter assay and Western blotting. Its functional role in OC was also investigated with the siRNA approach. Lastly, we assessed the expression change of miRNA-205-3p and its newly identified target in human OC tissues. miR-205-3p was downregulated in five human OC lines tested. Over-expressing miR-205-3p reduced OC cell proliferation and migration. MAPK10 was identified as a direct target of miR-205-3p. Knocking down MAPK10 suppressed OC cell growth and migration. In contrast, knocking down miR-205-3p promoted clonogenicity of primary ovary cells. In clinical samples, miR-205-3p and MAPK10 expressed inversely in accordance with their expression patterns in OC cells. miR-205-3p was shown as a novel tumor suppressor in OC via inhibiting the MAPK10 pathway. This new finding may inspire new personalized treatment for OC.
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http://dx.doi.org/10.1007/s43032-019-00047-yDOI Listing
January 2020

FGF21 Protects against Aggravated Blood-Brain Barrier Disruption after Ischemic Focal Stroke in Diabetic db/db Male Mice via Cerebrovascular PPARγ Activation.

Int J Mol Sci 2020 Jan 28;21(3). Epub 2020 Jan 28.

Clinical Neuroscience Research Center, Department of Neurosurgery, School of Medicine, Tulane University, New Orleans, LA 70112, USA.

Recombinant fibroblast growth factor 21 (rFGF21) has been shown to be potently beneficial for improving long-term neurological outcomes in type 2 diabetes mellitus (T2DM) stroke mice. Here, we tested the hypothesis that rFGF21 protects against poststroke blood-brain barrier (BBB) damage in T2DM mice via peroxisome proliferator-activated receptor gamma (PPARγ) activation in cerebral microvascular endothelium. We used the distal middle cerebral occlusion (dMCAO) model in T2DM mice as well as cultured human brain microvascular endothelial cells (HBMECs) subjected to hyperglycemic and inflammatory injury in the current study. We detected a significant reduction in PPARγ DNA-binding activity in the brain tissue and mRNA levels of BBB junctional proteins and PPARγ-targeting gene and in cerebral microvasculature at 24 h after stroke. Ischemic stroke induced a massive BBB leakage two days after stroke in T2DM mice compared to in their lean controls. Importantly, all abnormal changes were significantly prevented by rFGF21 administration initiated at 6 h after stroke. Our in vitro experimental results also demonstrated that rFGF21 protects against hyperglycemia plus interleukin (IL)-1β-induced transendothelial permeability through upregulation of junction protein expression in an FGFR1 activation and PPARγ activity elevation-dependent manner. Our data suggested that rFGF21 has strong protective effects on acute BBB leakage after diabetic stroke, which is partially mediated by increasing PPARγ DNA-binding activity and mRNA expression of BBB junctional complex proteins. Together with our previous investigations, rFGF21 might be a promising candidate for treating diabetic stroke.
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http://dx.doi.org/10.3390/ijms21030824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037567PMC
January 2020

Bioaccumulation and Trophic Transfer of Emerging Organophosphate Flame Retardants in the Marine Food Webs of Laizhou Bay, North China.

Environ Sci Technol 2019 Nov 6;53(22):13417-13426. Epub 2019 Nov 6.

Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology , Dalian University of Technology , Dalian , 116024 , China.

Despite the increase in production, usage, and discharge of organophosphate flame retardants (OPFRs), little information is available about their bioaccumulation and trophic transfer in the marine food web. In this study, seawater, sediment, and marine species (10 fish and 9 invertebrate species) collected from Laizhou Bay, North China, were analyzed to investigate the levels, bioaccumulation, and biomagnification of OPFRs in a marine food web. Of 20 OPFRs screened for, 17 were quantifiable in seawater, sediment, and organisms. The ∑OPFRs concentrations ranged from 0.2 to 28.4 ng/L in seawater, 0.1-96.9 ng/g dry weight in sediment, and 21.1-3510 ng/g lipid weight in organisms. Benthic fish accumulated more OPFRs than pelagic fish and invertebrates. A linear and significant increase of bioaccumulation factors with increasing lipophilicity of OPFRs was observed ( = 0.63, < 0.05), and the biota-sediment accumulation factors increased with hydrophobicity up to log  = 4.59 and then decreased with increase in log . Trophic magnification factors of OPFRs ranged from 1.06 to 2.52, indicating biomagnification potential of OPFRs in a marine food web. This study provides important insight into the biomagnification potential of OPFRs and suggests further investigation on this group of chemicals.
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http://dx.doi.org/10.1021/acs.est.9b03687DOI Listing
November 2019

[Effect of utilization of child health services on the nutrition and growth status of infants in poor rural areas in Sichuan and Gansu Province].

Wei Sheng Yan Jiu 2019 Sep;48(5):745-750

Department of Health Related Social and Behavioral Science, West China School of Public Health, Sichuan University, Chengdu 610041, China.

Objective: To investigate the current situation of the utilization of infant and young child health services in pool rural areas where minorities gathered in Sichuan and Gansu Province and analyze its effect on infant nutrition and growth.

Methods: We choosed 1065 infants and young children aged≤24 months with their mother as the subjects by multistage cluster random sampling in October to November 2014 in poor rural areas where ethnic minorities gathered in Sichuan and Gansu Province. Questionnaires were used to obtain the data of demography and utilization of maternal and child health services, physical examination to obtain the data of growth status, and hemoglobin detection to obtain the hemoglobin level. The relationship between the nutrition and growth status and maternal and child health service utilization was analyzed by the unconditioned Logistic regression analysis.

Results: Among the surveyed infants and young children, the underweight rate was 3. 38%, the stunting rate was 10. 52%, and the wasting rate was 2. 25%, the malnutrition prevalence was 12. 58%, and the anemia prevalence was 52. 68%. The children's physical examination rate was 37. 09%, and the systematic management( frequency of physical examination reached the standard) rate of children was 12. 68%. The rate of prophylactic inoculation was 92. 30%, and the rate of reaching the standard of vaccination frequency was 48. 73%. After adjusting the confounding factors, the result showed that utilization of vaccination( OR = 0. 41, 95% CI0. 23-0. 74) and vaccination frequency reaching the standard( OR = 0. 58, 95%CI 0. 36-0. 92) were protective factors for infant malnutrition. Children 's physical examinations frequency reaching the standard( OR = 0. 52, 95%CI 0. 35-0. 79) was a protective factor for infant anemia.

Conclusion: Reasonable utilization of health services can reduce the poor result of nutrition and growth of infants in poor rural areas where ethnic minorities gather in Sichuan and Gansu provinces.
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September 2019

Correlates of School Children's Handwashing: A Study in Tibetan Primary Schools.

Int J Environ Res Public Health 2019 09 3;16(17). Epub 2019 Sep 3.

Department of Health and Social Behavior Science, West China School of Public Health, Sichuan University, Chengdu 610041, China.

Hand hygiene, including handwashing by children, has been reported to contribute to the prevention of various infectious conditions. This study aims to explore the correlates of handwashing behavior among 1690 fourth to sixth grade primary school students in 19 Tibetan primary schools (Golog, Qinghai, China). The theory of reasoned action (TRA) was applied. Data was collected by questionnaire. Structural equation modeling (SEM) analysis showed that students' attitude (β = 0.22, 95% CI 0.13-0.31) and subjective norms in terms of compliance to teachers', parents' and peers' suggestions to wash hands (β = 0.09, 95% CI 0.01-0.18) were directly associated with students' handwashing behavior. Students' knowledge (β = 0.04, 95% CI 0.03-0.07) had an indirect association with handwashing behavior, mediated by students' attitudes and subjective norms. Subjective norms (β = 0.12, 95% CI 0.07-0.17) were also indirectly correlated with handwashing through students' attitudes. Therefore, our study supported the theory of reasoned action through our findings that students' attitude and knowledge, and also attitudes from teachers, parents and peers were correlated with student handwashing behavior. Students reported higher level of compliance to teachers than to their parents and classmates. Based on this information, we recommend teacher-involved participatory hygiene education to promote students' handwashing behaviors in areas at high risk for infectious diseases that can be prevented by handwashing.
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http://dx.doi.org/10.3390/ijerph16173217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747304PMC
September 2019

Synthesis and application of a "turn on" fluorescent probe for glutathione based on a copper complex of coumarin hydrazide Schiff base derivative.

Bioorg Chem 2019 10 3;91:103176. Epub 2019 Aug 3.

Xinxiang Key Laboratory of Forensic Science Evidence, School of Forensic Medicine, Xinxiang Medical University, Jinsui Road No. 601, Xinxiang 453003, Henan Province, PR China. Electronic address:

Discrimination and quantification of intracellular biothiols, such as cysteine (Cys), homocysteine (Hcy), glutathione (GSH) under physiological conditions is significant for academic research and disease diagnosis. A new fluorescent probe (complex 1-Cu) for discriminate detection of GSH was prepared by copper ions coordinate with coumarin carbohydrazide Schiff base derivative 1. In suitable buffer solution (CHCN: HEPES = 3:2, v/v) and under appropriate pH condition (pH = 7.2-7.4), the UV-vis spectroscopy experiments showed that compound 1 and copper ion exhibited a 1:1 ratio binding mode and moderate binding ability. Fluorescence quenching of compound 1 was observed when it complexed with Cu ions. An obviously fluorescence restoration appeared after addition of GSH to the solution of probe, which also exhibited a highly selectivity relative to cysteine (Cys) and homocysteine (Hcy) in the amino acid competitive experiments. The minimum detection limit was calculated to 0.12 μM by fluorescent method, which was distinctly below the physiological concentration of GSH in live cells. Its biological application to detect the endogenous GSH was further proved by the HepG2 cell fluorescence image test.
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http://dx.doi.org/10.1016/j.bioorg.2019.103176DOI Listing
October 2019

Use of maternal health services among women in the ethnic rural areas of western China.

BMC Health Serv Res 2019 Mar 19;19(1):179. Epub 2019 Mar 19.

The Freeman Spogli Institute for International Studies, Stanford University, Stanford, CA, USA.

Background: The use of maternal health services can markedly promote the maternal health and safety, but there has been a low utilization rate in the ethnic rural areas of western China. Furthermore, the correlated factors have not been well studied. This study aims to assess factors related to the use of maternal health services among women in these areas.

Methods: A cross-sectional study of 68 villages in China's western Sichuan province was conducted in September 2014. All qualifying women from each sample village were involved. A structured questionnaire was administrated in households through face-to-face interviews by trained enumerators to obtain information of use of maternal health services and related factors. Structural equation modeling (SEM) was used to evaluate the direct and indirect relationships between use of maternal health services and correlated factors.

Results: A total of 760 women from 68 villages were enrolled. The proportion of antenatal care (ANC), hospital delivery and postpartum visits were 68.94, 48.29 and 28.42% respectively. The SEM analysis demonstrated that social economic status (SES) (β= - 0.75, β< 0.01), ANC (β=0.13, β< 0.01), and time from home to the nearest hospital (β= - 0.09, β< 0.05), were positively correlated to hospital delivery and postpartum care visits, while maternal care knowledge and perceived quality of hospital care did not have direct correlation. For ANC, SES (β= - 0.36, β< 0.01), time from home to the nearest hospital (β= - 0.13, β< 0.05), knowledge on maternal care (β=0.12, β< 0.01) and perceived quality of hospital care (β=0.10, β< 0.01) were all directly correlated factors. Treating ANC as an intermediate variable showed the indirect relationship that perceived quality of hospital care (β=0.01, β< 0.01) and maternal care knowledge (β=0.02, β< 0.01) had with hospital delivery and postpartum care rates.

Conclusions: Use of maternal health services is low among women in ethnic rural areas. ANC has important direct and intermediate effects on subsequent use of hospital delivery and postpartum care. Improving ANC behavior should be a priority of maternal health care reforms. Given the long travel times for these women, reforms must also prioritize breaking down practical barriers that prevent this population from accessing care.
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http://dx.doi.org/10.1186/s12913-019-3996-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425603PMC
March 2019

Uptake and translocation of organophosphate flame retardants (OPFRs) by hydroponically grown wheat (Triticum aestivum L.).

Ecotoxicol Environ Saf 2019 Jun 14;174:683-689. Epub 2019 Mar 14.

Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.

The increasing load of organophosphate flame retardants (OPFRs) has generated wide concerns about their potential residues in aquatic environments. The uptake and translocation of fourteen OPFRs by wheat (Triticum aestivum L.) were studied under hydroponic conditions. The results revealed that OPFRs were removed from hydroponic solution by wheat, and the removal processes followed first-order kinetics. After 10 days, the removal efficiencies were in a range of 57.9 ± 3.8%-63.8 ± 5.6%. The potential for translocation of these OPFRs from the roots to foliage was also assessed. OPFRs with relatively higher hydrophobicity were more likely taken up by roots, and OPFRs with lower hydrophobicity were more prone to be translocated. Root concentration factors (RCFs), transpiration stream concentration factors (TSCFs), and foliage/root concentration factors (FRCFs) were calculated. Furthermore, significant correlations were found between RCF, FRCF or TSCF values of OPFRs and log K (p < 0.05), and translocation of OPFRs depended on their physicochemical properties. The findings of this study develop better understanding of accumulation and translocation of OPFRs in plants, which is valuable for environmental and human health assessments of such kind of contaminants.
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http://dx.doi.org/10.1016/j.ecoenv.2019.03.029DOI Listing
June 2019

Structural Equation Modeling (SEM) of Cysticercosis in School-Aged Children in Tibetan Rural Farming Areas of Western China: Implications for Intervention Planning.

Int J Environ Res Public Health 2019 03 4;16(5). Epub 2019 Mar 4.

Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Neurocysticercosis (NCC) significantly contributes to morbidity in developing countries. We recently published a study of prevalence and risk factors in school-aged children in three mountainous areas in Sichuan province of western China. Using structural equation modeling (SEM) on data from that study to guide intervention planning, here we examine risk factors grouped into three broad interventional categories: sociodemographics, human behavior, and sources of pork and pig husbandry. Because neuroimaging is not easily available, using SEM allows for the use of multiple observed variables (serological tests and symptoms) to represent probable NCC cases. Data collected from 2608 students was included in this analysis. Within this group, seroprevalence of cysticercosis IgG antibodies was 5.4%. SEM results showed that sociodemographic factors ( = 0.33, < 0.05), sources of pork and pig husbandry ( = 0.26, < 0.001), and behavioral factors ( = 0.33, < 0.05) were all directly related to probable NCC in school-aged children. Sociodemographic factors affected probable NCC indirectly via sources of pork and pig husbandry factors ( = 0.07, < 0.001) and behavioral variables ( = 0.07, < 0.001). Both sociodemographic factors ( = 0.07, < 0.05) and sources of pork and pig husbandry factors ( = 0.10, < 0.01) affected probable NCC indirectly via behavioral variables. Because behavioral variables not only had a large direct effect but also served as a critical bridge to strengthen the effect of sociodemographics and sources of pork and pig husbandry on probable NCC, our findings suggest that interventions targeting behavioral factors may be the most effective in reducing disease.
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http://dx.doi.org/10.3390/ijerph16050780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427563PMC
March 2019

NAMPT overexpression alleviates alcohol-induced hepatic steatosis in mice.

PLoS One 2019 22;14(2):e0212523. Epub 2019 Feb 22.

School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China.

Nicotinamide phosphoribosyltransferase (NAMPT) is a rate-limiting enzyme in mammalian nicotinamide adenine dinucleotide (NAD)+ biosynthesis. Through its NAD+-biosynthetic activity, NAMPT influences the activity of NAD+-dependent enzymes, such as sirtuins. NAMPT is able to modulate processes involved in the pathogenesis of non-alcohol induced fatty liver disease (NAFLD), but the roles NAMPT plays in development of alcoholic liver disease (ALD) still remain unknown. Here, we show that ethanol treatment suppresses the expression of Nampt in hepatocytes. Consistently, chronic ethanol administration also reduces Nampt expression in the mouse liver. We next demonstrate that hepatocytes infected with Ad-NAMPT adenovirus exhibit significantly elevated intracellular NAD+ levels and decreased ethanol-induced triglyceride (TG) accumulation. Similarly, adenovirus-mediated overexpression of NAMPT in mice ameliorates ethanol induced hepatic steatosis. Moreover, we demonstrate that SIRT1 is required to mediate the effects of NAMPT on reduction of hepatic TG accumulation and serum ALT, AST levels in ethanol-fed mice. Our results provide important insights in targeting NAMPT for treating alcoholic fatty liver disease.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212523PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386387PMC
November 2019

Distribution of Cu fraction in sediments using Suaeda heteroptera - Nereis succinea combination: A greenhouse study.

Int J Phytoremediation 2019 18;21(2):129-137. Epub 2019 Jan 18.

b Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, School of Fisheries and Life Science , Dalian Ocean University , Dalian , PR China.

A knowledge of the total amount of heavy metals is not enough to assess the environmental impact of polluted soils. Therefore, the determination of Cu fractions in sediment is important to evaluate its behavior in the environment and its mobilization capacity. The distribution of Cu (II) fractions in the sediment was studied in a laboratory simulation experiment. The results indicated that the distribution of Cu fractions was related to the concentration of Cu in sediments. In the Suaeda heteroptera group, the content of exchangeable and carbonate-bound were reducing, while the Fe-Mn oxide- and organic matter-bound were basically raising. In the Nereis succinea group, the content of Fe-Mn oxide- and organic matter-bound were from raising to reducing, and the residual was basically reducing with the increasing Cu concentrations. Generally speaking, the existence of N. succinea could enhance the uptake of Cu in the coastal sediment planted with S. heteroptera. When S. heteroptera and N. succinea in combination, effect of S. heteroptera and N. succinea on Cu fractions in sediments was significantly higher than the two working singly.
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http://dx.doi.org/10.1080/15226514.2018.1488807DOI Listing
September 2019
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