Publications by authors named "Qingwen Huang"

20 Publications

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Assessment of Human Exposure to Five Mycotoxins in China by Biomonitoring Approach.

Toxins (Basel) 2021 Oct 28;13(11). Epub 2021 Oct 28.

Key Laboratory of Protected Horticultural Technology, Institute for Agro-Food Standards and Testing Technology, Academy of Agricultural Sciences, Shanghai 201403, China.

This biomonitoring study was conducted to investigate the concentration levels of five mycotoxins in urine samples from 269 healthy volunteers living in the Yangtze River Delta, China. Alternariol (AOH), alternariol monomethyl ether (AME), tenuazonic acid (TeA) and tentoxin (TEN) were detected in 38.3%, 48.7%, 63.9% and 23.4% of urine samples with the concentrations ranging from 0.057 to 45.8 ng/mL, 0.020 to 0.802 ng/mL, 0.050 to 80.6 ng/mL and 0.021 to 0.939 ng/mL, respectively. Altenuene (ALT) was not detected in any urine sample. Based on the urinary concentrations, the probable daily intake (PDI) values of mycotoxins were calculated, and 100%, 99.2-100%, 0.372% and 1.12% of participants exceeded the threshold of toxicological concern (TTC) values for AOH, AME, TeA and TEN, respectively. This study revealed high potential health risks related to the contaminations of major mycotoxins in China and highlighted the necessity for more toxicological studies to provide better basis for further comprehensive risk assessments.
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http://dx.doi.org/10.3390/toxins13110762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625692PMC
October 2021

High expression predicts poor prognosis in patients with Head and Neck Squamous Cell Carcinomas.

J Cancer 2021 5;12(13):3887-3899. Epub 2021 May 5.

Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Overexpression of the membrane protein SEC61 translocon gamma subunit (SEC61G) has been observed in a variety of cancers; however, its role in head and neck squamous cell carcinomas (HNSCC) is unknown. This study aimed to elucidate the relationship between and HNSCC based on data from The Cancer Genome Atlas (TCGA) database. Data for HNSCC patients were collected from TCGA and the expression level of was compared between paired HNSCC and normal tissues using the Wilcoxon rank-sum test. The relationship between clinicopathologic features and expression was also analyzed using the Wilcoxon rank-sum test and logistic regression. Receiver operating characteristic (ROC) curves were generated to evaluate the value of as a binary classifier using the area under the curve (AUC value). The association of clinicopathologic characteristics with prognosis in HNSCC patients was assessed using Cox regression and the Kaplan-Meier methods. A nomogram, based on Cox multivariate analysis, was used to predict the impact of on prognosis. Functional enrichment analysis was performed to determine the hallmark pathways associated with differentially expressed genes in HNSCC patients exhibiting high and low expression. The expression of was significantly elevated in HNSCC tissues compared to normal tissues (P < 0.001). The high expression of was significantly correlated with the T stage, M stage, clinical stage, mutation status, mutation status, primary therapy outcome, and cervical lymph node dissection (all P < 0.05). Meanwhile, ROC curves suggested the significant diagnostic ability of for HNSCC (AUC = 0.923). Kaplan-Meier survival analysis showed that patients with HNSCC characterized by high expression had a poorer prognosis than patients with low expression (hazard ratio = 1.95, 95% confidence interval 1.48-2.56, P < 0.001). Univariate and multivariate analyses revealed that was independently associated with overall survival (P = 0.027). Functional annotations indicated that SEC61G is involved in pathways related to translation and regulation of SLITs/ROBOs expression, SRP-dependent co-translational protein targeting to the membrane, nonsense-mediated decay, oxidative phosphorylation, and Parkinson's disease. plays a vital role in HNSCC progression and prognosis; it may, therefore, serve as an effective biomarker for the prediction of patient survival.
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http://dx.doi.org/10.7150/jca.51467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176234PMC
May 2021

A Multifunctional N-Doped Cu-MOFs (N-Cu-MOF) Nanomaterial-Driven Electrochemical Aptasensor for Sensitive Detection of Deoxynivalenol.

Molecules 2021 Apr 13;26(8). Epub 2021 Apr 13.

College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China.

Deoxynivalenol (DON) is one of the most common mycotoxins in grains, causing gastrointestinal inflammation, neurotoxicity, hepatotoxicity and embryotoxicity, even at a low quantity. In this study, a facile electrochemical aptasensor was established for the rapid and sensitive determination of DON based on a multifunctional N-doped Cu-metallic organic framework (N-Cu-MOF) nanomaterial. The N-Cu-MOF, with a large specific surface area and good electrical conductivity, served not only as an optimal electrical signal probe but also as an effective supporting substrate for stabilizing aptamers through the interactions of amino (-NH) and copper. Under the optimal conditions, the proposed sensor provided a wide linear concentration range of 0.02-20 ng mL (R = 0.994), showing high sensitivity, with a lower detection limit of 0.008 ng mL, and good selectivity. The sensor's effectiveness was also verified in real spiked wheat samples with satisfactory recoveries of 95.6-105.9%. The current work provides a flexible approach for the rapid and sensitive analysis of highly toxic DON in food samples and may also be easily extended to detect other hazardous substances with alternative target-recognition aptamers.
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http://dx.doi.org/10.3390/molecules26082243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069659PMC
April 2021

Exposure Assessment of Multiple Mycotoxins and Cumulative Health Risk Assessment: A Biomonitoring-Based Study in the Yangtze River Delta, China.

Toxins (Basel) 2021 02 1;13(2). Epub 2021 Feb 1.

Institute for Agro-Food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Laboratory of Quality and Safety Risk Assessment for Agro-Products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.

The extensive exposure to multiple mycotoxins has been demonstrated in many countries; however, realistic assessments of the risks related to cumulative exposure are limited. This biomonitoring study was conducted to investigate exposure to 23 mycotoxins/metabolites and their determinants in 227 adults (aged 20-88 years) in the Yangtze River Delta, China. Eight mycotoxins were detected in 110 urine samples, and multiple mycotoxins co-occurred in 51/227 (22.47%) of urine samples, with deoxynivalenol (DON), fumonisin B1 (FB1), and zearalenone (ZEN) being the most frequently occurring. For single mycotoxin risk assessment, FB1, ZEN, aflatoxin B1 (AFB1), and ochratoxin A (OTA) all showed potential adverse effects. However, for the 12 samples containing DON and ZEN, in which none had a hazard risk, the combination of both mycotoxins in two samples was considered to pose potential endocrine disrupting risks to humans by hazard index (HI) method. The combined margin of exposure (MOE) for AFB1 and FB1 could constitute a potential health concern, and AFB1 was the main contributor. Our approach provides a blueprint for evaluating the cumulative risks related to different types of mycotoxins and opens a new horizon for the accurate interpretation of epidemiological health outcomes related to multi-mycotoxin exposure.
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http://dx.doi.org/10.3390/toxins13020103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912756PMC
February 2021

A flexible assay strategy for non-glucose targets based on sulfhydryl-terminated liposomes combined with personal glucometer.

Biosens Bioelectron 2021 Mar 16;175:112884. Epub 2020 Dec 16.

Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Laboratory of Quality and Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai, 201403, China. Electronic address:

The personal glucose meter (PGM) is one of the most successful point-of-care (POC) testing devices. It is simple, robust and inexpensive, but cannot be easily adapted to analytes other than glucose. We report a novel chemical conjugation-based assay strategy, using rational design of chemically-derivatized glucose-encapsulating liposomes, to repurpose a PGM, taking an important mycotoxin patulin as the model analyte. Sulfhydryl (-SH) was proposed for the first time as a specific functional group for efficient recognition of patulin. Multifunctional sulfhydryl-terminated glucose-encapsulating liposomes (G-LIP-SH) were synthesized in a simple, single step, which efficiently captured patulin by covalent bonding, and interacted strongly with [email protected] nanoparticles. Magnetic removal of nanoparticles efficiently and selectively separated patulin-derivatized from un-derivatized G-LIP-SH, permitting the latter to be lysed and the released glucose measured by PGM. The PGM signal was inversely proportional to the patulin concentration, over the range of 0.1-50 ng mL (R = 0.995) with a detection limit of 0.05 ng mL (S/N = 3). This approach overcame interference from endogenous glucose, other mycotoxins and metal ions, allowing the analysis of a wide range of sample matrices and showed high specificity, acceptable reproducibility, good accuracy and optimal applicability. Other derivatization chemistries will enable this approach to be adapted to analytes with a wide range of chemical structures, to facilitate development of rapid, portable, user-friendly and cost-effective assays applicable to diverse analytes and sample matrices.
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http://dx.doi.org/10.1016/j.bios.2020.112884DOI Listing
March 2021

Reduced graphene oxide-zinc oxide nanocomposite as dispersive solid-phase extraction sorbent for simultaneous enrichment and purification of multiple mycotoxins in Coptidis rhizoma (Huanglian) and analysis by liquid chromatography tandem mass spectrometry.

J Chromatogr A 2020 Sep 1;1630:461515. Epub 2020 Sep 1.

College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China. Electronic address:

In the current study, a robust dispersive solid-phase extraction (dSPE) strategy using reduced graphene oxide-zinc oxide (rGO-ZnO) nanocomposite as the sorbent was proposed for separation, purification and enrichment of 12 mycotoxins in Coptidis rhizoma (Huanglian). The targeted mycotoxins included aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, aflatoxin M1, alternariol-methylether, mycophenolic acid, ochratoxin A, penitrem A, nivalenol, zearalenone and zearalanone. The rGO-ZnO nanocomposite was successfully synthesized through hydrothermal process by a modified Hummers method, and further characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), FTIR spectroscopy, ultraviolet-visible spectroscopy and X-ray diffraction (XRD). Several key parameters affecting the performance of the dSPE approach were extensively investigated, and after optimization, acetonitrile/water/formic acid (80/19/1, v/v/v) as the extraction solution, 2% acetonitrile as the adsorption solution, 15 mg rGO-ZnO as the sorbent, n-hexane as the washing solution, and methanol/formic acid (99/1, v/v) as the desorption solution presented an excellent purification and enrichment efficiency. Under the optimal dSPE procedure followed by analysis with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), adequate linearity (R ≥ 0.991), high sensitivity (limit of quantification in the range of 0.09-0.41 µg kg), acceptable recovery (70.3-105.7%) and satisfactory precision (RSD 1.4-15.0%) were obtained. The analysis of 12 selected mycotoxins was also carried out in real Coptidis rhizoma (Huanglian) samples for applicability evaluation of the established method.
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http://dx.doi.org/10.1016/j.chroma.2020.461515DOI Listing
September 2020

Class A1 scavenger receptors mediated macrophages in impaired intestinal barrier of inflammatory bowel disease.

Ann Transl Med 2020 Feb;8(4):106

Department of Gastroenterology, Affiliated Zhongshan Hospital of Xiamen University, Xiamen 361004, China.

Background: This study was to investigate the cytokines and phenotype of macrophages pre-treated with class A1 scavenger receptor (SR-A1) antibody in vitro and the influence on apoptotic pathway of colonic epithelial cells, and to explore the role of SR-A1 mediated macrophages in impaired intestinal barrier of inflammatory bowel diseases (IBDs).

Methods: Mouse macrophage RAW264.7 was pre-treated with SR-A1 antibody in the presence of lipopolysaccharide (LPS). Transwell system was employed for co-culture of RAW264.7 and Caco-2 in the presence of LPS and IFN-γ, with or without SR-A1 antibody pre-treatment. The percentage of F4/80CD11c macrophages, apoptosis rate of Caco-2 cells, and expression of apoptosis and tight junction proteins in Caco-2 cells was determined.

Results: Pre-treatment with SR-A1 antibody up-regulated IL-10 expression in RAW264.7, whereas down-regulated the expression of TNF and iNOS. Immunofluorescence staining indicated the upregulation of NF-κB p-p56 after LPS stimulation was significantly inhibited in the presence of SR-A1 antibody. The increase in p-JNK expression was inhibited by SR-A1 antibody. Transwell assay showed the percentage of F4/80CD11c macrophages and apoptotic Caco-2 cells increased after treatment with LPS and IFN-γ, which could be reversed in the presence of SR-A1 antibody. The induction of cleaved caspase-3 and claudin-1 in Caco-2 cells was also suppressed when SR-A1 antibody pre-treatment.

Conclusions: Pre-treatment with SR-A1 antibody can inhibit inflammatory response in LPS-induced macrophages in a NF-κB dependent manner. Pre-treatment with SR-A1 antibody also inhibits M1 phenotype expression of macrophages, and attenuates the pro-apoptotic effect on colonic epithelial cells and disruption of intestinal barrier integrity induced by macrophages.
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http://dx.doi.org/10.21037/atm.2019.12.107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049059PMC
February 2020

Dual-target electrochemical aptasensor based on co-reduced molybdenum disulfide and Au NPs (rMoS-Au) for multiplex detection of mycotoxins.

Biosens Bioelectron 2020 Feb 15;150:111894. Epub 2019 Nov 15.

Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Laboratory of Quality and Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China. Electronic address:

Multiple mycotoxin contamination has posed health risks in the area of food safety. In this study, co-reduced molybdenum disulfide and gold nanoparticles (rMoS-Au) were designed and used for the first time as an efficient platform endowing electrochemical electrodes with superior electron transfer rates, large surface areas and strong abilities to firmly couple with large amounts of different aptamers. After further modification with thionine (Thi) and 6-(Ferrocenyl) hexanethiol (FC6S), a platform enabling sensitive, selective and simultaneous determination of two important mycotoxins, zearalenone (ZEN) and fumonisin B1 (FB1), was achieved. The established aptasensor showed excellent linear relationships (R > 0.99) when ZEN and FB1 concentrations were in the range of 1 × 10-10 ng mL and 1 × 10-1 × 10 ng mL, respectively. High sensitivity of ZEN and FB1 with a limit of detection as low as 5 × 10 ng mL was obtained with excellent selectivity and stability. The effectiveness of the aptasensor was verified in real maize samples, and satisfactory recoveries were attained. The established platform could be easily expanded to other aptamer-based multiplex screening protocols in biochemical research and clinical diagnosis.
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http://dx.doi.org/10.1016/j.bios.2019.111894DOI Listing
February 2020

Label-Free Fluorescent Aptasensor for Ochratoxin-A Detection Based on CdTe Quantum Dots and (-Methyl-4-pyridyl) Porphyrin.

Toxins (Basel) 2019 07 28;11(8). Epub 2019 Jul 28.

Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Laboratory of Quality and Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.

With the widespread contamination of ochratoxin A (OTA), it is of significant importance for detecting OTA in foods and traditional Chinese medicine (TCM). In this study, a novel label-free fluorescent aptasensor utilizing the interaction between OTA-triggered antiparallel G-quadruplex and (N-methyl-4-pyridy) porphyrin (TMPyP) for the rapid and sensitive determination of OTA was established. The fluorescence of CdTe quantum dots (QDs) could be quenched by TMPyP. In the presence of analyte (OTA), the aptamer could recognize OTA and transform from a random coil to the antiparallel G-quadruplex. The interaction between G-quadruplex and TMPyP could release CdTe QDs from TMPyP, and thus recover the fluorescence of CdTe QDs. Under optimized conditions, the detection limit of the designed aptasensor was 0.16 ng mL, with a linear range of 0.2 to 20 ng mL. Furthermore, this aptasensor showed high selectivity toward OTA against other structural analogs and other mycotoxins, and was successfully applied in samples. The presented aptasensor for OTA detection could be a promising tool for the field monitoring of food and TCM.
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http://dx.doi.org/10.3390/toxins11080447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724026PMC
July 2019

Thin-layer MoS and thionin composite-based electrochemical sensing platform for rapid and sensitive detection of zearalenone in human biofluids.

Biosens Bioelectron 2019 Apr 8;130:322-329. Epub 2019 Feb 8.

Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Laboratory of Quality and Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China. Electronic address:

In this study, a facile electrochemical sensing platform based on thin-layer MoS and thionin (MoS-Thi) composites was constructed for the sensitive and rapid detection of zearalenone (ZEA) in human biofluids. MoS-Thi composites with a thin-layer MoS structure and large specific surface area were synthesized via a simple ultrasound exfoliation method. To further enhance the performance of the platform but without increasing the complexity of the fabrication process, ZEA antibodies were modified with Pt nanoparticles (Pt NPs) using an environmentally friendly method, where they had a much wider linear range and four times higher sensitivity compared with the original ZEA monoclonal antibody (ZEA-MAb). Under the optimal conditions, the electrochemical responses of the MoS-Thi composite-based immunosensor were linear (R = 0.9915) when the ZEA concentrations ranged from 0.01 to 50 ng mL. The proposed immunosensor could detect ZEA at concentrations as low as 0.005 ng mL with excellent selectivity. The immunosensor exhibited acceptable stability with high reproducibility and accuracy, and it was applied successfully for determining ZEA in human plasma and urine samples. Our findings indicated that the proposed MoS-Thi composite-based electrochemical sensing platform provides a new approach for the rapid and sensitive bioanalysis of ZEA, and it may also be extended to other mycotoxins for multiplex detection.
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http://dx.doi.org/10.1016/j.bios.2019.02.003DOI Listing
April 2019

Molecularly Imprinted Poly(thionine)-Based Electrochemical Sensing Platform for Fast and Selective Ultratrace Determination of Patulin.

Anal Chem 2019 03 6;91(6):4116-4123. Epub 2019 Mar 6.

Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Laboratory of Quality and Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture , Shanghai Academy of Agricultural Sciences , 1000 Jingqi Road , Shanghai 201403 , P. R. China.

An innovative approach based on a surface functional monomer-directing strategy for the construction of a sensitive and selective molecularly imprinted electrochemical sensor for patulin recognition is described. A patulin imprinted platinum nanoparticle (PtNP)-coated poly(thionine) film was grown on a preformed thionine tailed surface of PtNP-nitrogen-doped graphene (NGE) by electropolymerization, which provided high capacity and fast kinetics to uptake patulin molecules. Thionine acted not only as a functional monomer for molecularly imprinted polymer (MIP), but also as a signal indicator. Enhanced sensitivity was obtained by combining the excellent electric conductivity of PtNPs, NGE, and thionine with multisignal amplification. The designed sensor displayed excellent performance for patulin detection over the range of 0.002-2 ng mL (R = 0.995) with a detection limit of 0.001 ng mL for patulin. In addition, the resulting sensor showed good stability and high repeatability and selectivity. Furthermore, the feasibility of its applications has also been demonstrated in the analysis of real samples, providing novel tactics for the rational design of MIP-based electrochemical sensors to detect a growing number of deleterious substances.
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http://dx.doi.org/10.1021/acs.analchem.8b05791DOI Listing
March 2019

Development of a QuEChERS-Based UHPLC-MS/MS Method for Simultaneous Determination of Six Toxins in Grapes.

Toxins (Basel) 2019 02 1;11(2). Epub 2019 Feb 1.

Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.

A simple and reliable analytical method for the simultaneous determination of alternariol (AOH), altenuene (ALT), tentoxin (TEN), altenusin (ALS), tenuazonic acid (TeA), and alternariol monomethyl ether (AME) in grapes was developed by ultra-high-performance liquid chromatography⁻tandem mass spectrometry (UHPLC-MS/MS). A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure with the extraction by acetonitrile and purification by sodium chloride (0.5 g) and anhydrous magnesium sulfate (0.5 g) was established to recover the six toxins. After validation by determining the linearity (² > 0.99), recovery (77.8⁻101.6%), sensitivity (limit of detection in the range of 0.03⁻0.21 μg kg, and limit of quantification in the range of 0.09⁻0.48 μg kg), and precision (relative standard deviation (RSD) ≤ 12.9%), the analytical method was successfully applied to reveal the contamination state of toxins in grapes. Among 56 grape samples, 40 (incidence of 71.4%) were contaminated with toxins. TEN was the most frequently found mycotoxin (37.5%), with a concentration range of 0.10⁻1.64 μg kg, followed by TeA (28.6%) and AOH (26.8%). ALT (10.7%), AME (3.6%), and ALS (5.4%) were also detected in some samples. To the best of our knowledge, this is the first report about the toxins contamination in grapes in China.
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http://dx.doi.org/10.3390/toxins11020087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410285PMC
February 2019

Synthesis and anticancer activity of novel rapamycin C-28 containing triazole moiety compounds.

Arch Pharm (Weinheim) 2018 Nov 25;351(11):e1800123. Epub 2018 Oct 25.

Fujian University of Traditional Chinese Medicine, Fuzhou, P. R. China.

Rapamycin is an mTOR allosteric inhibitor with multiple functions such as immunosuppressive, anticancer, and lifespan prolonging activities. Its C-43 semi-synthetic derivatives temsirolimus and everolimus have been used as mTOR targeting anticancer drugs in the clinic. Following our previous research on antitumor rapalogs modified on the C-43 position, 13 novel rapamycin triazole hybrids (6a-g, 7a-f) were designed and synthesized on the C-28 position of rapamycin via Huisgen's reaction. Anticancer assays indicated that the targeted derivatives containing phenyl and 4-methylphenyl groups showed an obvious raise in anticancer activity. On the contrary, the compounds with methoxyl, amine, and halogen groups on the benzene ring displayed lower anticancer activity. Compound 6c, as the most active compound, showed a stronger inhibition effect as compared with rapamycin for almost all of the tested cell lines (p < 0.01), except PC-3. Meanwhile, the effect of 6c on inducing apoptosis and cell cycle arrest in A549 cells was more powerful than that of rapamycin. In addition, 6c inhibited the phosphorylation of mTOR and its downstream key kinases 4EBP1 and p70S6K1 in A549 cells, indicating that 6c also effectively inhibits the mTORC1 signaling pathway as rapamycin. On the basis of these findings, 6c may have the potential to be developed as a new mTOR inhibitor against specific cancers.
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http://dx.doi.org/10.1002/ardp.201800123DOI Listing
November 2018

Facile Fabrication of Honeycomb-like Carbon Network-Encapsulated Fe/FeC/FeO with Enhanced Li-Storage Performance.

ACS Appl Mater Interfaces 2018 Oct 11;10(42):35994-36001. Epub 2018 Oct 11.

School of Chemistry and Materials Science, Jiangsu Key Laboratory of Green Synthetic Chemistry for Functional Materials , Jiangsu Normal University , Xuzhou , Jiangsu 221116 , China.

Three-dimensional honeycomb-like carbon network-encapsulated Fe/FeC/FeO composites are constructed via a facile pyrolysis of ferrite nitrate-poly(vinyl pyrrolidone) precursors. The nanostructures of the composites form in terms of the iron catalysis in the pyrolysis process, which greatly depends on the reaction temperature and contents of raw materials. The Fe/FeC/FeO/C composite obtained at 700 °C possesses a high surface area, outstanding structural stability, and fast electron/Li ion transportability. As the anode for lithium-ion batteries, it displays a high specific capacity (1295 mAh g at 0.2 A g), long cycling stability, and fast kinetics (345 mAh g after 500 cycles at 5 A g). Besides the nanostructures, the marriage of different components also contributes to the superior electrochemical performance. The integral carbon matrix supplies a fast electron/Li transportation pathway. Fe/FeC acts as an electrocatalyst in the electrode, which may bring extra capacity. The satisfied performance and facile fabrication with low cost make it a competitive material in practical applications.
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http://dx.doi.org/10.1021/acsami.8b13331DOI Listing
October 2018

Reduced graphene oxide and gold nanoparticle composite-based solid-phase extraction coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry for the determination of 9 mycotoxins in milk.

Food Chem 2018 Oct 8;264:218-225. Epub 2018 May 8.

Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory of Protected Horticultural Technology, Laboratory of Quality and Safety Risk Assessment for Agro-products (Shanghai), Ministry of Agriculture, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China. Electronic address:

A reliable solid-phase extraction (SPE) procedure using reduced graphene oxide and gold nanoparticle (rGO/Au) composite as sorbent was proposed for purification and enrichment of aflatoxin B (AFB), aflatoxin M (AFM), ochratoxin A (OTA), zearalenone (ZEA), α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), zearalanone (ZAN), α-zearalanol (α-ZAL) and β-zearalanol (β-ZAL) in milk. Main parameters affecting the performance of SPE procedure were thoroughly investigated. The optimized conditions included 2% acetonitrile in water as the loading solution, 5% methanol in water as the washing solution, and methanol/acetonitrile/formic acid (50/49/1, v/v/v) as the elution solvent. Satisfactory linearity (R ≥ 0.992), high sensitivity (limit of quantification in the range of 0.02-0.18 ng mL), adequate recovery (70.2-111.2%), and acceptable precision (RSD, 2.0-14.9%) were obtained when the optimal sample pretreatment protocol was combined with ultra-high-performance liquid chromatography-tandem mass spectrometry detection. The applicability of the validated method was further verified in real milk samples.
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http://dx.doi.org/10.1016/j.foodchem.2018.05.041DOI Listing
October 2018

The lncRNA-HOXA-AS2/EZH2/LSD1 oncogene complex promotes cell proliferation in pancreatic cancer.

Am J Transl Res 2017 15;9(12):5496-5506. Epub 2017 Dec 15.

Department of Gastroenterology, Zhongshan Hospital, Xiamen UniversityXiamen, People's Republic of China.

Emerging evidence have indicated that long non-coding RNAs (lncRNAs) play crucial roles in cancer development and progression. Previous studies have suggested that lncRNA-HOXA cluster antisense RNA 2 (HOXA-AS2) is involved in tumorigenesis of several cancers. However, little is known about the alteration and biological functions of HOXA-AS2 in pancreatic cancer (PC). The purpose of this study is to identify the role of HOXA-AS2 in PC. Here, we provided evidence that lncRNA HOXA-AS2 was up-regulated in PC tissues. In addition, Loss-of-function experiments revealed that HOXA-AS2 knockdown effectively suppressed proliferation by blocking the cell cycle transition and caused apoptosis of PC cells in vitro and in vivo. Mechanistically, we found that HOXA-AS2 directly interacted with enhancer of zeste homolog 2 (EZH2) and lysine specific demethylase 1 (LSD1), which promoted PC cell growth ability. Collectively, our findings demonstrated that lncRNA-HOXA-AS2/EZH2/LSD1 complex may function as an oncogene in PC cell proliferation, and also provides a potential therapy target for PC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752899PMC
December 2017

Interleukin-25 enhances the capacity of mesenchymal stem cells to induce intestinal epithelial cell regeneration.

Am J Transl Res 2017 15;9(12):5320-5331. Epub 2017 Dec 15.

Department of Gastroenterology, Affiliated Zhongshan Hospital of Xiamen UniversityXiamen 361004, Fujian, China.

Background: This study aimed to investigate the influence of IL-25 on the capacity of mesenchymal stem cells (MSCs) to induce intestinal epithelial cell regeneration.

Methods: The CD4IL-25R cells and LGR5IL-25R cells in colonic mucosa of Crohn's disease (CD) patients, ulcerative colitis (UC) patients and healthy controls were detected by immunofluorescence staining, and the CD4IL-25R cells in peripheral blood were detected by flow cytometry. Rat MSCs were separated and stimulated with IL-25. Then, MSCs were further incubated in IL-25-free DMEM for 24 h, and this DMEM was collected as conditioned medium (CM). IEC-6 cells were divided into 3 groups: experimental group (CM and TNF-α), control group (DMEM and TNF-α) and negative control group (DMEM).

Results: The CD4IL-25R cells and LGR5IL-25R cells significantly increased in the colonic mucosa of active CD patients and UC patients compared with IBD patients in remission and healthy controls. The CD4IL-25R cells reduced in peripheral blood of IBD patients, which was inversely correlated with inflammatory markers (ESR and CRP). CM facilitated the migration and proliferation of IEC-6 cells in the presence of TNF-α. The protein expression of AKT, p38 and ERK increased in IEC-6 cells after treatment with CM and TNF-α.

Conclusion: IL-25R is involved in Th-related mucosal inflammation and proliferation of intestinal stem cells in IBD. IL-25 enhances the capacity of MSC to induce intestinal epithelial cell regeneration, and MSC therapy with IL-25 may be a new direction for IBD treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752884PMC
December 2017

Knockdown of pseudogene derived from lncRNA DUXAP10 inhibits cell proliferation, migration, invasion, and promotes apoptosis in pancreatic cancer.

J Cell Biochem 2018 04 5;119(4):3671-3682. Epub 2018 Jan 5.

Department of Gastroenterology, Zhongshan Hospital, Xiamen University, Xiamen, Fujian, China.

Current evidence suggests that pseudogene derived lncRNAs may be important players in human cancer progression. Our previous study showed that DUXAP10 could promote cell proliferation in colorectal cancer. However, the clinical significance and potential role of DUXAP10 in human pancreatic cancer (PC) has not been uncovered. In this study, we found that DUXAP10 was overexpressed in PC tissues compared with normal tissues. DUXAP10 expression was significantly higher in patients with an advanced TNM stage and positive lymph node metastasis. Bioinformatic analysis showed that cell cycle progression was increased in patients with high DUXAP10 expression. In vitro and in vivo assays of DUXAP10 alterations revealed a complex integrated phenotype affecting cell growth, apoptosis, migration, and invasion. Mechanistic studies revealed that DUXAP10 has a crucial role in G2/M arrest. We further showed that DUXAP10 regulated PC cell proliferation through interact with RNA-binding protein EZH2 and LSD1. Overall, our findings indicates that DUXAP10 is an oncogenic lncRNA that promotes PC proliferation and metastasis.
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http://dx.doi.org/10.1002/jcb.26578DOI Listing
April 2018

Interleukin-25 primed mesenchymal stem cells achieve better therapeutic effects on dextran sulfate sodium-induced colitis via inhibiting Th17 immune response and inducing T regulatory cell phenotype.

Am J Transl Res 2017 15;9(9):4149-4160. Epub 2017 Sep 15.

Department of Gastroenterology, Zhongshan Hospital Affiliated to Xiamen UniversityXiamen 361004, Fujian Province, China.

Aim: This study aimed to investigate the anti-inflammatory mechanism of IL-25 mediated mesenchymal stem cells (MSC) treatment for inflammatory bowel disease (IBD) in a DSS-induced rat colitis model.

Methods: Rats with DSS-induced colitis were divided into control and treatment groups: normal control group (rats fed with water), DSS group (rats fed with DSS solution), MSC group (DSS-treated rats injected intravenously with GFP-MSCs), IL-25-MSC group (DSS-treated rats injected intravenously with IL-25 primed GFP-MSCs), and mesalazine group (DSS-treated rats fed with mesalazine).

Results: In IL-25-MSC group, therapeutic efficacy (clinical symptoms) was better than in MSC group, but comparable to mesalazine group. In IL-25-MSC group and mesalazine group, fewer infiltrating inflammatory cells and lower pathological score were observed in the intestine. The FOXP3 cells and IL-4 cells decreased, but IL-17A cells and IFN-γ cells increased in the peripheral blood and colonic mucosa after DSS induced colitis, and these phenomena were reversed by MSC or mesalazine treatment. IL-17A cells reduced and FOXP3 cells increased in IL-25-MSC group as compared with MSC group. The expressions of Ki67 and LGR5 were significantly elevated in MSC treatment groups as compared with normal control group, DSS group, and mesalazine group. Definite GFP positive cells were not observed in the intestine of MSC-treated rats.

Conclusion: IL-25 primed MSCs exert improved therapeutic effects on the intestinal inflammation of IBD rats which may be related to the inhibition of Th17 immune response and induction of T Regulatory cell phenotype. Thus, IL-25 may be an attractive candidate for MSC-based therapy of IBD.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5622258PMC
September 2017

Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy enhances real-time in vivo diagnosis of adenomatous polyps during colonoscopy.

J Biophotonics 2016 Apr 7;9(4):333-42. Epub 2015 Apr 7.

Optical Bioimaging Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore, 117576, Singapore.

Colorectal cancer can be prevented if detected early (e.g., precancerous polyps-adenoma). Endoscopic differential diagnosis of hyperplastic polyps (that have little or no risk of malignant transformation) and adenomas (that have prominent malignant latency) remains an unambiguous clinical challenge. Raman spectroscopy is an optical vibrational technique capable of probing biomolecular changes of tissue associated with neoplastic transformation. This work aims to apply a fiber-optic simultaneous fingerprint (FP) and high wavenumber (HW) Raman spectroscopy technique for real-time in vivo assessment of adenomatous polyps during clinical colonoscopy. We have developed a fiber-optic Raman endoscopic technique capable of simultaneously acquiring both the FP (i.e., 800-1800 cm(-1)) and HW (i.e., 2800-3600 cm(-1)) Raman spectra from colorectal tissue subsurface (<200 µm) for real-time assessment of colorectal carcinogenesis. In vivo FP/HW Raman spectra were acquired from 50 patients with 17 colorectal polyps during clinical colonoscopy. Prominent Raman spectral differences (p < 0.001) were found between hyperplastic (n = 118 spectra), adenoma (n = 184 spectra) that could be attributed to changes in inter- and intra-cellular proteins, lipids, DNA and water structures and conformations. Simultaneous FP/HW Raman endoscopy provides a diagnostic sensitivity of 90.9% and specificity of 83.3% for differentiating adenoma from hyperplastic polyps, which is superior to either the FP or HW Raman technique alone. This study shows that simultaneous FP/HW Raman spectroscopy technique has the potential to be a clinically powerful tool for improving early diagnosis of adenomatous polyps in vivo during colonoscopic examination.
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http://dx.doi.org/10.1002/jbio.201400141DOI Listing
April 2016
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