Publications by authors named "Qingtao Wang"

73 Publications

Reciprocal interaction between SIRT6 and APC/C regulates genomic stability.

Sci Rep 2021 Jul 9;11(1):14253. Epub 2021 Jul 9.

MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

SIRT6 is an NAD-dependent deacetylase that plays an important role in mitosis fidelity and genome stability. In the present study, we found that SIRT6 overexpression leads to mitosis defects and aneuploidy. We identified SIRT6 as a novel substrate of anaphase-promoting complex/cyclosome (APC/C), which is a master regulator of mitosis. Both CDH1 and CDC20, co-activators of APC/C, mediated SIRT6 degradation via the ubiquitination-proteasome pathway. Reciprocally, SIRT6 also deacetylated CDH1 at lysine K135 and promoted its degradation, resulting in an increase in APC/C-CDH1-targeted substrates, dysfunction in centrosome amplification, and chromosome instability. Our findings demonstrate the importance of SIRT6 for genome integrity during mitotic progression and reveal how SIRT6 and APC/C cooperate to drive mitosis.
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http://dx.doi.org/10.1038/s41598-021-93684-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270898PMC
July 2021

Comparability of thyroid-stimulating hormone immunoassays using fresh frozen human sera and external quality assessment data.

PLoS One 2021 15;16(6):e0253324. Epub 2021 Jun 15.

Department of Clinical Laboratory, Beijing Chaoyang Hospital, Beijing Center for Clinical Laboratories, The Third Clinical Medical College of Capital Medical University, Beijing, P.R. China.

Background: This study aimed to assess the comparability among assays using freshly frozen human sera and external quality assessment (EQA) data in China.

Methods: Twenty-nine serum samples and two commercial EQA materials, obtained from the National Center for Clinical Laboratories (NCCL), were analyzed in triplicate using eight routine TSH assays. The commutability of commercial EQA materials (NCCL materials) was evaluated in accordance with the CLSI EP30-A and IFCC bias analysis. Median values obtained for the NCCL EQA materials were used to determine the systematic and commutability-related biases among immunoassays through back-calculation. The comparability of TSH measurements from a panel of clinical samples and NCCL EQA data was determined on the basis of Passing-Bablok regression. Furthermore, human serum pools were used to perform commutable EQA.

Results: NCCL EQA materials displayed commutability among three or five of seven assay combinations according CLSI or IFCC approach, respectively. The mean of systematic bias ranged from -13.78% to 9.85% for the eight routine TSH assays. After correcting for systematic bias, averaged commutability-related biases ranged between -42.26% and 12.19%. After correction for systematic and commutability -related biases, the slopes indicating interassay relatedness ranged from 0.801 to 1.299 using individual human sera, from 0.735 to 1.254 using NCCL EQA data, and from 0.729 to 1.115 using pooled human serum EQA(the commutable EQA).

Conclusions: The harmonization of TSH measurement is challenging; hence, systematic and commutability-related biases should be determined and corrected for accurate comparisons among assays when using human individual serum and the commercial EQA materials.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253324PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205121PMC
June 2021

Analytical characterization and clinical performance evaluation of a new point-of-care testing system for high-sensitivity cardiac troponin I assay.

Ann Clin Biochem 2021 Jul 8:45632211027604. Epub 2021 Jul 8.

Department of Clinical Laboratory, Beijing Chao-yang Hospital, Capital Medical University, Beijing, People's Republic of China.

Background: We aimed to evaluate the analytical performance and clinical diagnostic accuracy of the SuperFlex point-of-care testing (POCT) high-sensitivity cardiac troponin I (hs-cTnI) assay system.

Method: The imprecision, the limit of blank, the limit of detection, the limit of quantitation, linearity and comparability were assessed as per the Clinical and Laboratory Standards Institute guidelines. Also, the 99th-percentile reference value and diagnostic accuracy were evaluated.

Results: The reproducibility and total imprecision were 1.52-1.92% and 2.69-2.92%, respectively. Limit of blank and limit of detection were 1 ng/L and 1.8 ng/L, respectively, and limit of quantitation was 12 ng/L at 10% coefficient of variation (CV). The results met the requirements of linearity, and the correlation coefficient was 0.996. The SuperFlex POCT results had good agreement with those obtained by the Siemens Advia 2400. The CV% was 7.24% at the 99th percentile concentration (p99th) of 25.6 ng/L (95% confidence interval: 22.0-33.3 ng/L) from 620 healthy subjects. The sex-partitioned CV% and p99th were 7.15% at 27 ng/L (males;  = 308) and 7.35% at 24 ng/L (females;  = 312), respectively ( < 0.0001). The hs-cTnI detection rate of all observed healthy individuals from limit of detection to 99th was 82.57% by the SuperFlex POCT assay, 89.90% for the males and 75.48% for the females. The sensitivity, specificity, positive predictive value and negative predictive value of diagnostic performance for acute myocardial infarction were 100%, 81.25%, 57% and 100%, respectively.

Conclusions: The SuperFlex POCT system showed the analytical performance characteristics required for enabling the clinical use of a hs-cTnI assay.
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http://dx.doi.org/10.1177/00045632211027604DOI Listing
July 2021

Protective effects of natural products against drug-induced nephrotoxicity: A review in recent years.

Food Chem Toxicol 2021 Jul 11;153:112255. Epub 2021 May 11.

Innovation Center for Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. Electronic address:

Drug-induced nephrotoxicity (DIN) is a major cause of kidney damage and is associated with high mortality and morbidity, which limits the clinical use of certain therapeutic or diagnostic agents, such as antineoplastic drugs, antibiotics, immunosuppressive agents, non-steroidal anti-inflammatory drugs (NSAIDs), and contrast agents. However, in recent years, a number of studies have shown that many natural products (NPs), including phytochemicals, various plants extracts, herbal formulas, and NPs derived from animals, confer protective effects against DIN through multi-targeting therapeutic mechanisms, such as inhibition of oxidative stress, inflammation, apoptosis, fibrosis, and necroptosis, regulation of autophagy, maintenance of cell polarity, etc., by regulating multiple signaling pathways and novel molecular targets. In this review, we summarize and discuss the protective effects and mechanisms underlying the action of NPs against DIN found in recent years, which will contribute to the development of promising renal protective agents.
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http://dx.doi.org/10.1016/j.fct.2021.112255DOI Listing
July 2021

Trueness assessment of routine electrolytes measuring systems using the candidate reference method by ion chromatography.

Scand J Clin Lab Invest 2021 04 11;81(2):85-91. Epub 2021 Feb 11.

Department of Clinical Laboratory, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of China.

Electrolytes for sodium, potassium, magnesium, and calcium are important serum ions that are frequently assayed in clinical laboratories. In this study, we assessed the trueness of routine analytical systems for four cations using an inexpensive candidate reference method aimed to promote the standardization of serum electrolyte detection. An ion chromatography (IC) method with Cesium as an internal standard was developed and evaluated. The residual clinical serum samples at Chaoyang Hospital were collected and prepared into three human serum pools of electrolytes, which were used for the trueness evaluation of five routine analytical systems. Furthermore, the agreement between routine methods and the IC method was verified using 40 individual human samples. The recovery rates of sodium, potassium, magnesium and calcium were 99.69%, 100.34%, 100.43% and 99.89%, respectively. The intra-batch standard deviation and intra-laboratory precision of NIST SRM 956c were all less than 1% for the four ions. The certified values were within the validation range, and the deviation between the results and the certified values were less than 0.5%. The three serum pools were homogeneous and stable. All routine systems aligned with the IC method for four cations and achieved the analytical quality specifications for potassium and magnesium at 3 different concentrations. The developed IC method is simple, practical, accurate, and precise, which can be used as a candidate reference method for serum electrolytes measurement. Five routine analytical systems for electrolytes measurement had the acceptable bias for potassium and magnesium and their results showed good concordance.
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http://dx.doi.org/10.1080/00365513.2020.1855469DOI Listing
April 2021

Nicotinamide mononucleotide inhibits hepatic stellate cell activation to prevent liver fibrosis via promoting PGE degradation.

Free Radic Biol Med 2021 01 19;162:571-581. Epub 2020 Nov 19.

MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University, Beijing, China. Electronic address:

Liver fibrosis is a reversible wound-healing response to acute or chronic liver injury that can progress to cirrhosis and liver cancer. Finding new strategies for prevention and management of liver fibrosis is urgently needed. It is known that hepatic stellate cell (HSC) is the primary source of extracellular matrix that drives liver fibrosis progression. Herein, we carried out a comprehensive secretome profiling to identify NMN-induced changes in secretory proteins and found that NMN suppressed the secretion of profibrotic protein and oxidoreductase in activated HSC (LX-2) cells, while real-time quantitative PCR analysis revealed that NMN downregulated profibrotic gene expression, resulting in HSC inactivation. Next, we demonstrated that nicotinamide mononucleotide (NMN) reduced the accumulation of liver extracellular matrix in thioacetamide (TAA) and carbon tetrachloride (CCl) induced mouse models for liver fibrosis. Furthermore, we determined that NMN inhibited oxidation-mediated 15-PGDH degradation to promote prostaglandin E degradation and suppress HSC activation. In summary, our results propose that NMN supplementation is a new therapeutic approach for liver fibrosis prevention.
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http://dx.doi.org/10.1016/j.freeradbiomed.2020.11.014DOI Listing
January 2021

Sacrifice Template Strategy to the Fabrication of a Self-Cleaning Nanofibrous Membrane for Efficient Crude Oil-in-Water Emulsion Separation with High Flux.

ACS Appl Mater Interfaces 2020 Nov 11;12(47):53484-53493. Epub 2020 Nov 11.

Key Laboratory of Eco-functional Polymer Materials of the Ministry of Education, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, P. R. China.

The superhydrophilic/underwater superoleophobic membrane materials have attracted considerable attention in oil/water separation. However, most materials are extremely susceptible to pollution during oil-water separation, which drastically restricts their widespread applications. Herein, a momordica-charantia-like nanofibrous membrane (MCNM) with underwater superoleophobic performance was fabricated through a sacrifice template strategy by the electrospinning solution of zeolitic imidazolate framework-8 (ZIF-8) and polyacrylonitrile particles. The opened voids and wrinkles left after removing the template of nanocrystals ZIF-8 not only increased the porosity and roughness of the as-prepared fibrous membrane but also tremendously improved the underwater superoleophobicity. Therefore, the as-prepared MCNM showed excellent self-cleaning performance toward crude oil under water, avoiding the decrease of the separation efficiency and flux caused by membrane fouling during oil-water separation. Meanwhile, the separation efficiency of various surfactant-stabilized oil-in-water emulsions was higher than 99.6% with a flux up to 1580 ± 30 L m h solely driven by gravity. Moreover, no obvious wrinkles and cracks were observed on the resulted nanofibrous membrane after the sand impact and bent testing. More importantly, the as-prepared MCNM still maintained exceptional underwater superoleophobicity in harsh environment (3.5 wt % NaCl, 4 M HCl, 50 °C hot water) even after ultrasound for 1 h. The robust mechanical and chemical stability makes the antifouling MCNM exhibit tremendous potential for practical applications in dealing with oily wastewater in the future.
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http://dx.doi.org/10.1021/acsami.0c15387DOI Listing
November 2020

Comparative proteomic analysis identifies biomarkers for renal aging.

Aging (Albany NY) 2020 11 6;12(21):21890-21903. Epub 2020 Nov 6.

MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University, Beijing, China.

Proteomics have long been applied into characterization of molecular signatures in aging. Due to different methods and instrumentations employed for proteomic analysis, inter-dataset validation needs to be performed to identify potential biomarkers for aging. In this study, we used comparative proteomics analysis to profile age-associated changes in proteome and glutathionylome in mouse kidneys. We identified 108 proteins that were differentially expressed in young and aged mouse kidneys in three different datasets; from these, 27 proteins were identified as potential renal aging biomarkers, including phosphoenolpyruvate carboxykinase (Pck1), CD5 antigen-like protein (Cd5l), aldehyde dehydrogenase 1 (Aldh1a1), and uromodulin. Our results also showed that peroxisomal proteins were significantly downregulated in aged mice, whereas IgGs were upregulated, suggesting that peroxisome deterioration might be a hallmark for renal aging. Glutathionylome analysis demonstrated that downregulation of catalase and glutaredoxin-1 (Glrx1) significantly increased protein glutathionylation in aged mice. In addition, nicotinamide mononucleotide (NMN) administration significantly increased the number of peroxisomes in aged mouse kidneys, indicating that NMN enhanced peroxisome biogenesis, and suggesting that it might be beneficial to reduce kidney injuries. Together, our data identify novel potential biomarkers for renal aging, and provide a valuable resource for understanding the age-associated changes in kidneys.
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http://dx.doi.org/10.18632/aging.104007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695359PMC
November 2020

Comparability of four clinical laboratory measurement methods for GGT and commutability of candidate reference materials.

J Clin Lab Anal 2020 Dec 11;34(12):e23557. Epub 2020 Sep 11.

Department of Clinical Laboratory, Beijing Chao-yang Hospital, Capital Medical University, Beijing, China.

Background: This study was conducted to evaluate the progress in the standardization of the gamma-glutamyl transferase (GGT) to achieve metrological traceability of routine in vitro diagnosis (IVD) medical devices.

Methods: We collected 25 single fresh frozen serum samples for GGT analysis. Candidate reference materials (RMs), calibrators, internal quality controls (IQC), and external quality assessment (EQA) materials from the National Center for Clinical Laboratory (NCCL), Beijing Center for Clinical Laboratory (BCCL), and College of American Pathologists (CAP) were randomly added to these serum samples. A total of 42 samples were examined using IFCC reference method and four different IVD medical devices to perform the comparability and commutability study.

Results: The four IVD medical devices achieved trueness assessment within the measurement range. Linear analysis showed the agreement of Siemens ADVIA 2400, Hitachi 7600-020/BioSino, Beckman AU 5800, and Roche Cobas 501 with the reference method. These assay pairs were comparable at the medical decision levels. The GGT in-house candidate RMs, and Beckmann and Roche calibrators were all within the limits of the 95% prediction intervals, the commutability of BioSino calibrators was indeterminate, and some internal and external quality controls were not commutable for comparisons of certain IVD medical devices vs the reference method.

Conclusions: By comparing with the reference method, we found that performance of GGT conventional measurement systems to be traceable to the higher order references was improved. The commutable materials for calibration and trueness controls of routine methods were significant to promote the standardization of GGT analysis.
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http://dx.doi.org/10.1002/jcla.23557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755790PMC
December 2020

Glutathionylation Decreases Methyltransferase Activity of PRMT5 and Inhibits Cell Proliferation.

Mol Cell Proteomics 2020 11 31;19(11):1910-1920. Epub 2020 Aug 31.

MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, MOE Key Laboratory of Bioinformatics, Beijing, China. Electronic address:

Glutathionylation is an important posttranslational modification that protects proteins from further oxidative damage as well as influencing protein structure and activity. In the present study, we demonstrate that the cysteine-42 residue in protein arginine N-methyltransferase 5 (PRMT5) is glutathionylated in aged mice or in cells that have been exposed to oxidative stress. Deglutathionylation of this protein is catalyzed by glutaredoxin-1 (Grx1). Using mutagenesis and subsequent biochemical analyses, we show that glutathionylation decreased the binding affinity of PRMT5 with methylosome protein-50 (MEP50) and reduced the methyltransferase activity of PRMT5. Furthermore, overexpression of PRMT5-C42A mutant caused a significant increase in histone methylation in HEK293T and A549 cells and promoted cell growth, whereas overexpression of the PRMT5-C42D mutant, a mimic of glutathionylated PRMT5, inhibited cell proliferation. Taken together, our results demonstrate a new mechanism of regulation of PRMT5 methyltransferases activity and suggest that PRMT5 glutathionylation is partly responsible for reactive oxygen species-mediated cell growth inhibition.
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http://dx.doi.org/10.1074/mcp.RA120.002132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664116PMC
November 2020

Silencing Promotes Mitochondrial Biogenesis, Increases Putrescine Toxicity and Decreases Cell Motility to Suppress ccRCC Progression.

Int J Mol Sci 2020 Aug 18;21(16). Epub 2020 Aug 18.

MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

Carbonic anhydrase IX (CA9), a pH-regulating transmembrane protein, is highly expressed in solid tumors, and particularly in clear cell renal cell carcinoma (ccRCC). The catalytic mechanisms of CA9 are well defined, but its roles in mediating cell migration/invasion and survival in ccRCC remain to be determined. Here, we confirmed that the mRNA expression of in ccRCC was significantly higher than that in para-carcinoma tissues from analysis of the datasets in The Cancer Genome Atlas. knockdown upregulated oxidative phosphorylation-associated proteins and increased mitochondrial biogenesis, resulting in the reversal of the Warburg phenotype and the inhibition of cell growth. Our study revealed that knockdown upregulated mitochondrial arginase 2 (ARG2), leading to the accumulation of putrescine, which suppressed ccRCC proliferation. Surfaceomics analysis revealed that knockdown downregulated proteins associated with extracellular matrix (ECM)-receptor interaction and cell adhesion, resulting in decreased cell migration. silencing also downregulated amino acid transporters, leading to reduced cellular amino acids. Collectively, our data show that knockdown suppresses proliferation via metabolic reprogramming and reduced cell migration, reaffirming that CA9 is a potential therapeutic target for ccRCC treatment.
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http://dx.doi.org/10.3390/ijms21165939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460829PMC
August 2020

Establishment of reference intervals for pediatric complete capillary blood counts: A multicenter study in Beijing.

Int J Lab Hematol 2020 Oct 13;42(5):533-543. Epub 2020 Aug 13.

Department of Clinical Laboratory, Beijing Center for Clinical Laboratories, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Introduction: Clinical reference intervals represent the normal range of clinical parameters for distinguishing healthy and sick individuals, and they show some variation among different populations. Many reference intervals are still lacking for the pediatric population in China. Thus, the aim of this study was to establish and validate pediatric reference intervals for capillary blood cell counts.

Methods: A total of 9942 children were enrolled from 10 medical institutions in Beijing, China, for capillary complete blood count (CCBC) values according to the EP28-A3c guideline issued by the Clinical and Laboratory Standards Institute.

Results: Pediatric reference intervals for 17 CCBC parameters were established for children aged 6 months to 7 years. The red blood cell count and red blood cell distribution width were generally higher in males than in females, and the mean corpuscular hemoglobin and mean corpuscular volume were higher in females than in males. The red blood cell, hemoglobin, hematocrit, and neutrophil percentages increased while the percentage of lymphocytes decreased with age. The overall trends for each reference interval were relatively similar in different ethnic groups and regions in the world, but with variation in the upper and lower limits, which confirms the existence of racial and geographical differences. Further validation with 508 healthy subjects showed that the verified proportions were within 90.9%-100% of the reference intervals.

Conclusions: This study offers local reference intervals for CCBC values for the pediatric population in Beijing, China, and thus provides basic criteria for the diagnosis, treatment, and health assessment of childhood diseases in China.
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http://dx.doi.org/10.1111/ijlh.13150DOI Listing
October 2020

Establishment of Multiple Myeloma Diagnostic Model Based on Logistic Regression in Clinical Laboratory.

Clin Lab 2020 Aug;66(8)

Background: Due to the insidious onset of multiple myeloma (MM), missed diagnosis and misdiagnosis have a serious impact on the health of MM patients. Simple, rapid, and valid laboratory screening is critical for MM clinical diagnosis.

Methods: We used routine laboratory tests to establish a simple, inexpensive, and non-invasive diagnostic model for MM based on logistic regression. In the retrospective analysis, a total of 273 newly diagnosed MM inpatients and 288 non-MM participants, from January 2016 to December 2018 in Beijing Chaoyang hospital, Capital Medical University, were divided into training set and validation set. Age, gender, and the related routine laboratory tests for MM, including albumin (ALB), globulin (GLB), lactate dehydrogenase (LDH), creatinine (Cr), calcium (Ca2+), hemoglobin (Hb) and platelet (PLT), were analyzed by multivariate logistic regression to develop a diagnostic model.

Results: A diagnostic model was calculated using the formula MM index=-((-18×gender-3×ALB-Hb)/10), based on the logistic regression. The MM index [22 (20 - 25)] of MM patients was significantly lower than that of non-MM [30 (29 - 31)] in the training set (p < 0.001). It showed an excellent diagnostic performance in diagnosing MM through a receiver operating characteristic (ROC) curve, and its corresponding sensitivity, specificity, and area under the curve (AUC) were 95.6%, 96.7%, and 0.982 (0.968, 0.997), respectively. At a diagnostic risk threshold of 28, the model identified MM with a sensitivity of 95.6% and a specificity of 98.1% by using independent validation data. There was a significant positive correlation (r = 0.845, p < 0.001) between the DS grading and the MM index among all the participants.

Conclusions: The established diagnostic model of MM index can successfully identify newly diagnosed MM from healthy controls. The diagnostic model of MM index may also act as a predictor of the severity of MM without therapy.
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http://dx.doi.org/10.7754/Clin.Lab.2019.190832DOI Listing
August 2020

Iron ion irradiated BiTe nanosheets with defects and regulated hydrophilicity to enhance the hydrogen evolution reaction.

Nanoscale 2020 Aug;12(30):16208-16214

Key Laboratory of Evidence Science Research and Application of Gansu Province, Gansu University of Political Science and Law, Lanzhou 730070, China.

The introduction of defects can enhance the active sites on transition metal dichalcogenides, which can cause changes in crystal structures, and then lead to a change in the original catalytic performance. Herein, an efficient method of introducing defects was reported. In this method, high-energy iron ions were irradiated on the surface of Bi2Te3 nanosheets by ion irradiation technology, which resulted in the generation of a variety of defects. Compared to the original Bi2Te3 nanosheets, the Bi2Te3 nanosheets irradiated by iron ions showed significant improvement in the hydrogen evolution reaction performance in acidic solution. After the iron ion irradiation, the electric double layer capacitance of the Bi2Te3 nanosheets increased significantly, which indicated an increase in the number of active sites on the surface of Bi2Te3. Analysis of high-resolution transmission electron microscopy images reveals the occurrence of various defects on the surface of Bi2Te3 after the iron ion irradiation, which increased the active sites. Moreover, the conductivity of the iron ion-irradiated Bi2Te3 nanosheets was also significantly improved. It is noteworthy that iron ion irradiation changed the characteristic of the Bi2Te3 surface from hydrophobic to hydrophilic, which facilitated the release of hydrogen bubbles from the catalyst surface and exposed the active sites in time. At the same time, the damage caused by the large bubbles to the electrode material could be avoided, and the stability of the material was improved. This efficient iron ion irradiation method provides an innovative idea for the design of other high-efficient catalysts.
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http://dx.doi.org/10.1039/d0nr03256eDOI Listing
August 2020

Superhydrophobic ZIF-8-Based Dual-Layer Coating for Enhanced Corrosion Protection of Mg Alloy.

ACS Appl Mater Interfaces 2020 Aug 27;12(31):35453-35463. Epub 2020 Jul 27.

Research Center of Gansu Military and Civilian Integration Advanced Structural Materials, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, P. R. China.

Magnesium (Mg) and its alloys are regarded as the most promising engineering materials because of their unique property. However, the Mg alloys were easily corroded in humid environments, which restricted their wider applications. Herein, the superhydrophobic ZIF-8/PVDF/LDH (SZPL) double-layered coating was fabricated on Mg alloys via electrodeposition and dip-coating methods, which consisted of the underlying layered double hydroxide (LDH) transition structure and top superhydrophobic zeolitic imidazolate framework-8 (ZIF-8) layer. Besides, the LDH transition structure not only worked as a protection shield but also strengthened the binding force between the substrate and the top superhydrophobic ZIF-8 layer. The top superhydrophobic ZIF-8 layer could serve as an armor on the LDH layer to further prevent the corrosive ions from infiltrating the microporous defects. In addition, the as-prepared SZPL double-layered coating showed robust superhydrophobic and self-cleaning properties, which could block the electrolyte invasion. Furthermore, the electrochemical tests demonstrated that the SZPL coating highly enhanced the corrosion protection ability of Mg alloys. Moreover, the superhydrophobic ZIF-8-based coating could still retain excellent anticorrosion property after immersion in 3.5 wt % NaCl solution for 7 days. The enhanced anticorrosion ability was ascribed to the fact that a synergistic effect of the underlying LDH transition layer hindered the transmission of aggressive ions and the top superhydrophobic ZIF-8-based coating decreased the contact area of the substrate with corrosive solution. Therefore, such coatings offer a new strategy for fabricating excellent anticorrosive coatings with robust superhydrophobicity and self-cleaning performance on metal substrates.
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http://dx.doi.org/10.1021/acsami.0c09497DOI Listing
August 2020

Commutability of external quality assessment materials for point-of-care glucose testing using the Clinical and Laboratory Standards Institute and International Federation of Clinical Chemistry approaches.

J Clin Lab Anal 2020 Aug 27;34(8):e23327. Epub 2020 Apr 27.

Department of Laboratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Objectives: The aim of this study was to assess the commutability of three external quality assessment (EQA) materials for point-of-care (POC) glucose testing using two approaches, to identify suitable EQA materials to evaluate and monitor the quality of POC testing.

Methods: Commercial control materials (CCMs), pooled human serum samples (PHSs), and homemade human whole-blood samples (HWBs) were measured along with 33 individual clinical samples using five POC instruments and a Hitachi 7600 analyzer. Data were analyzed by Deming regression analysis with a 95% prediction interval as described in Clinical and Laboratory Standards Institute (CLSI) EP30-A, and by difference in bias analysis as described by the International Federation of Clinical Chemistry (IFCC) Working Group on Commutability.

Results: Using the CLSI approach, HWBs, CCMs, and PHSs were commutable with five, one, and two instruments, respectively. With the IFCC approach, HWBs were commutable with two instruments, while CCMs and PHSs were largely inconclusive or non-commutable on five instruments.

Conclusions: HWBs were commutable on all instruments by the CLSI approach and may be a suitable EQA material for POC testing. Although some results differed between the IFCC and CLSI approaches, both indicated that HWBs were far superior to CCMs and PHSs in commutability.
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http://dx.doi.org/10.1002/jcla.23327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439342PMC
August 2020

Phosphorus-doped CoTe/C nanoparticles create new Co-P active sites to promote the hydrogen evolution reaction.

Nanoscale 2020 Apr;12(16):9171-9177

Key Laboratory of Eco-Functional Polymer Materials of the Ministry of Education, Key Laboratory of Eco-Environmental Polymer Materials of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.

Doping has been widely recognized as an effective method for adjusting the performance of electrocatalysts. It can cause changes in the electronic structure of substances. Thereby, it can affect the intrinsic catalytic performance. Herein, we report a facile doping method in which phosphorus can be simultaneously doped into both CoTe2 and C. In the acidic solution, the hydrogen evolution reaction (HER) performance of the obtained P-CoTe2/C nanoparticles was significantly improved compared with that of undoped nanoparticles. At a current density of 10 mA cm-2, the overpotential decreased from 430 mV to 159 mV. Density functional theory (DFT) calculations show that phosphorus doping can produce new high activity Co-P catalytic sites. In addition, phosphorus can be doped into the carbon in the composite at the same time, which enhances the electrical conductivity of the composite. Moreover, in the process of calcination and doping, the electric double layer capacitance (Cdl) of the composite is significantly increased, which helps in exposing more active sites. This work has developed a multi-effect doping method that simultaneously increases the intrinsic activity, conductivity and active sites of the material. This method provides a new strategy for the performance regulation of other electrocatalysts.
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http://dx.doi.org/10.1039/d0nr00007hDOI Listing
April 2020

Evaluation of Uniformity and Stability of Frozen Mixed Serum Potassium Candidate Reference Materials.

Clin Lab 2020 Apr;66(4)

Background: We tested the uniformity and stability of candidate reference materials (RMs) for serum potassium and aimed to design RMs with better quality that can meet all clinical test requirements and effectively solve the quantity traceability transfer problem.

Methods: Three levels of frozen mixed serum potassium candidate RMs were prepared and packed in freezing tubes. RMs were determined in triplicate in 10 vials randomly selected from each level. A one-way analysis of variance was used to evaluate the uniformity using a ratio of the mean squares among groups to mean squares within groups F < F0.05 as the criteria. Stability was studied by synchronization; the short-term stability of the serum potassium in the transport conditions was observed for 30, 15, and 7 days at refrigeration (2 - 8°C), room temperature (18 - 25°C), and 37°C, respectively. By linear regression analysis of variance, the straight line was used as an empirical model. The criterion for judging is |b1| < t0.95, n - 2·sb1.

Results: Based on the statistical analysis using SPSS 17, the F values for the homogeneity tests of each level of the frozen mixed serum potassium RMs were 0.247, 0.117, and 0.162. These values were less than F0.05 (9, 20) = 2.39. When the short-term stability of the serum potassium was observed for 30, 12, and 4 days at 2 - 8°C, room temperature, and 37°C, respectively, |b1| < t0.95, n - 2·sb1, and instability was not observed.

Conclusions: The three levels of the frozen mixed serum potassium RMs have good uniformity. At 2 - 8°C, room temperature, and 37°C, the stability can be ensured for at least 30, 12, and 4 days, respectively. The serum potassium candidate RMs are sufficiently stable under these transportation conditions.
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http://dx.doi.org/10.7754/Clin.Lab.2019.190803DOI Listing
April 2020

Outstanding catalytic performance in the semi-hydrogenation of acetylene in a front-end process by establishing a "hydrogen deficient" phase.

Chem Commun (Camb) 2019 Dec;55(99):14910-14913

Industrial Catalysis Institute of Zhejiang University of Technology, State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technology, Hangzhou, 310014, P. R. China.

Pd-[Bmim][Cl] phase was immobilized onto Al2O3 to neutralize the excessive hydrogen in the gas phase to prevent the over-hydrogenation of acetylene, thereby achieving a high selectivity for ethylene.
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http://dx.doi.org/10.1039/c9cc06469aDOI Listing
December 2019

Ultrathin 2D TiCT MXene membrane for effective separation of oil-in-water emulsions in acidic, alkaline, and salty environment.

J Colloid Interface Sci 2020 Mar 18;561:861-869. Epub 2019 Nov 18.

Gansu International Scientific and Technological Cooperation Base of Water-retention Chemical Functional Materials, Northwest Normal University, Lanzhou 730070, China. Electronic address:

Layered TiCT MXene is a two-dimensional (2D) metal carbide based material with high adsorption capacity and hydrophilicity, which is beneficial for oil/water separation. Herein, the TiCT (T represents O, OH, and/or F) MXene membrane was obtained by depositing TiCT MXene 2D nanosheets carbides onto porous polyvinylidene fluoride (PVDF) membranes by vacuum filtration. The as-prepared TiCT MXene membrane exhibits excellent underwater superoleophobicity with oil contact angles (OCAs) close to 158° and oil sliding angles (OSAs) lower than 7°. In addition, the layered TiCT MXene membrane can separate a series of stable emulsions even emulsified crude oil-in-water mixtures, and displays excellent separation efficiency over 99.4% and high permeation flux of 887 L m h bar. Furthermore, the TiCT membrane displays superior durability to the corrosive liquids such as acidic, alkaline and salty, and can also effectively remove oil droplets from water in corrosive environment. This work provides a promising approach to prepare the ultrathin and layered 2D MXene TiCT membrane for separation of stable emulsified oil-in-water mixtures separation.
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http://dx.doi.org/10.1016/j.jcis.2019.11.069DOI Listing
March 2020

Different approaches for estimating measurement uncertainty: An effective tool for improving interpretation of results.

Clin Chim Acta 2020 Apr 13;503:223-227. Epub 2019 Nov 13.

Department of Laboratory Medicine, University Hospital of Padova, Padova, Italy. Electronic address:

Background: The estimation of measurement uncertainty (MU) in clinical laboratories is of crucial importance in improving laboratory testing quality and correctly interpreting results. However, it is difficult for clinical laboratories to reliably estimate MU since current guidelines and standards fail to clearly define and harmonize methods to be used for this purpose.

Aims: To propose a model for MU estimation in relation to test interpretation on the basis of three different scenarios.

Methods: MU estimation was evaluated regard to the inclusion of imprecision and bias components for different test purposes.

Results: Three scenarios were identified. The expanded uncertainty values were: 0.56 ng/L and 1.86 ng/L for troponin I at levels 4.95 ng/L and 20.60 ng/L, respectively; 9.4 μmol/L and 27.2 μmol/L for creatinine at levels 121.0 μmol/L and 390.0 μmol/L, respectively. 0.2 mmol/L and 0.3 mmol/L for potassium at levels 4.03 mmol/L and 6.35 mmol/L, respectively; 0.36 mmol/L for glucose at level of around 6 mmol/L. These values represent MU results estimated for the three scenarios, which contemplate test results used for 1a) short term patient monitoring, 1b) longer term patient monitoring, 2) comparison with reference intervals and 3) comparison with a clinical decision point.

Conclusions: Goal-based measurement uncertainty estimation in clinical laboratories provides an opportunity to improve laboratory testing quality and reduce risk in the interpretation of clinical results.
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http://dx.doi.org/10.1016/j.cca.2019.11.011DOI Listing
April 2020

Establishment of a reference procedure to measure urine-formed elements and evaluation of an automated urine analyzer.

Scand J Clin Lab Invest 2019 Dec 30;79(8):579-583. Epub 2019 Oct 30.

Department of Clinical Laboratory, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

A standardized reference method is needed to accurately and precisely measure urine-formed elements (UFEs; red blood cells [RBCs], white blood cells [WBCs], and squamous epithelial cells [sECs]). We compared the results from a standard method with those from an automated analyzer. Trained technicians used standardized bright-field microscopy of fresh non-centrifuged urine samples, and disposable 1 µl chambers. Fifteen experienced technicians from 5 hospitals (3 per hospital) each performed 6 manual counts of 10 different native urine samples using a manual chamber and standard methods. The sEC counts were at least 50/µL, and the coefficient of variation (CV) was less than 14%; the RBC and WBC counts were at least 200/µL and the CVs were less than 7%. The same samples were also analyzed 6 times using automated analyzers. The means, CVs, and biases were determined. The median CVs for the manual measurements were 6.4% (WBCs), 6.6% (RBCs), and 12.7% (sECs). The CVs of the automated analyzer were 4.7% (WBCs), 5.6% (RBCs), and 9.2% (sECs). Biases between the automated and manual methods were -2.9% to 5.0%(WBCs), -0.8% to 8.8% (RBCs) and -2.8% to 9.4% (sECs). The count mean values and expanded uncertainties of these counts were (224.5 ± 15.0) cells/µL, (234.2 ± 16.2) cells/µL, and (61.5 ± 7.9) cells/µL, respectively. The standardized manual method for measuring UFEs had high precision and accuracy, making it a suitable reference method. Use of this reference method to calibrate an automated analyzer improved the accuracy of automated analysis.
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http://dx.doi.org/10.1080/00365513.2019.1680860DOI Listing
December 2019

Highly Active AuCu-Based Catalysts for Acetylene Hydrochlorination Prepared Using Organic Aqua Regia.

Materials (Basel) 2019 Apr 22;12(8). Epub 2019 Apr 22.

Industrial Catalysis Institute, Laboratory Breeding Base of Green Chemistry-Synthesis Technology, Zhejiang University of Technology, Hangzhou 310014, China.

Development of a sustainable process for designing and synthesising an active and stable catalyst for hydrochlorination of acetylene is challenging, yet crucial, for industrial vinyl chloride monomer (VCM) production. Herein, direct synthesis of bimetallic AuCu catalysts using organic aqua regia (OAR) preparation methods was investigated. In comparison with conventional aqua regia (AR), bimetallic AuCu catalysts synthesised from OAR exhibit enhanced activity and stability. After careful characterisation of the catalyst samples using X-ray diffraction patterns (XRD), Scanning transmission electron microscopy (STEM), X-ray photoelectron spectroscopy (XPS), and Temperature-programmed desorption (TPD), this observation was justified for the following reasons: 1) the existence of sulphur and nitrogen atoms stabilised the cationic Au active sites, and 2) OAR helped to sustain the function of the Cu promotor by stabilising it. Advanced understanding on the importance of promoter stability has unveiled new perspectives for this research area.
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http://dx.doi.org/10.3390/ma12081310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515409PMC
April 2019

The Effects of Hybridization on the Flexural Performances of Carbon/Glass Interlayer and Intralayer Composites.

Polymers (Basel) 2018 May 20;10(5). Epub 2018 May 20.

College of Textiles, Donghua University, NO. 2999, Northern RenminRd, Songjiang District, Shanghai 201620, China.

The effects of hybridization on the flexural properties of interlayer and intralayer Carbon/Glass (C/G) composites were explored in this work. First, the theoretical analysis of stress distribution on interlayer hybrid composites was discussed. The experimental results showed that the layer structure is the critical factor affecting the flexural properties for interlayer hybrid composites, and the mixed ratio has no obvious impact. Since the carbon fiber is distributed at the top or bottom surface, the interlayer composites can obtain the maximum flexural modulus. Some structures can even achieve the same modulus as the pure carbon composites, and an excellent flexural strength can be attained with the carbon fiber located in the bottom layer. In terms of the intralayer hybrid composites, the fracture strain, flexural modulus, and flexural strength basically change linearly as the glass fiber content increases, which is consistent with the calculated values via the rule of mixture (ROM). Additionally, the C/G mixed ratio has a decisive effect on the flexural properties of intralayer hybrid composites; however, they are affected weakly by the layer structure. In general, some structures of the interlayer hybrid composites exhibit better flexural properties than that of the intralayer hybrid composites at the same C/G hybrid ratio; the alterations in layer structures make it possible to obtain excellent flexural properties for interlayer hybrid composites with less carbon fiber.
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http://dx.doi.org/10.3390/polym10050549DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415510PMC
May 2018

Compression Properties of Interlayer and Intralayer Carbon/Glass Hybrid Composites.

Polymers (Basel) 2018 Mar 21;10(4). Epub 2018 Mar 21.

College of Textiles, Donghua University, No. 2999, Northern Renmin Rd, Songjiang District, Shanghai 201620, China.

The compression properties and mechanisms of interlayer and intralayer Carbon/Glass (C/G) hybrid composites were investigated in this work. As revealed from the experimental results, the compression modulus increases linearly with the increase of carbon fiber content, following the rule of mixtures (ROM). The C/G hybrid ratio is regarded as the decisive factor for the compression modulus of hybrid composites. The positive mixing effect exists on compression strength for interlayer and intralayer hybrid composites, whereas the experimental values are above the theoretical calculation values. The compressive strength of interlayer hybrid composites taking on various hybrid structures differs largely at the same mixed ratio, at which the compressive strength of glass fiber sandwiching carbon fiber is higher than that of carbon fiber sandwiching glass fiber. Through comparing interlayer and intralayer hybrid composites, the impact exerted by layer structures on the compressive strength of interlayer hybrid composites is higher than that of intralayer hybrid composites, which leads to more designable characteristics for interlayer hybrid composites. This work makes it possible to optimize the compression strength of interlayer hybrid structures so that it achieves or basically exceeds pure carbon fiber composites.
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http://dx.doi.org/10.3390/polym10040343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414848PMC
March 2018

A pilot study for establishing quality indicators in molecular diagnostics according to the IFCC WG-LEPS initiative: preliminary findings in China.

Clin Chem Lab Med 2019 05;57(6):822-831

Department of Clinical Laboratory, Beijing Chaoyang Hospital, The Third Clinical Medical College of Capital Medical University, Beijing, P.R. China.

Background Quality indicators (QIs) are crucial tools in measuring the quality of laboratory services. Based on the general QIs of the Working Group "Laboratory Errors and Patient Safety (WG-LEPS)" of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), specific QIs have been established in order to monitor and improve the quality of molecular diagnostics, and to assess the detection level of associated disease. Methods A survey was conducted on 46 independent commercial laboratories in China, investigated using questionnaires and on-site inspections. Specific QIs established were mainly based on the specific laboratory work-flow for molecular diagnoses. The specific QI results from three volunteer laboratories were collected and used to validate their effectiveness. Results Of the 46 laboratories participating in the study, 44 (95.7%), conducted molecular diagnostics. Of 13 specific established QIs, six were priority level 1, and seven, priority level 3. At pre-evaluation of data from the three volunteering laboratories, it was found that the newly classified specific QIs had outstanding advantages in error identification and risk reduction. Conclusions Novel specific QIs, a promising tool for monitoring and improving upon the total testing process in molecular diagnostics, can effectively contribute to ensuring patient safety.
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http://dx.doi.org/10.1515/cclm-2018-0966DOI Listing
May 2019

Comparison of Tensile and Compressive Properties of Carbon/Glass Interlayer and Intralayer Hybrid Composites.

Materials (Basel) 2018 Jun 28;11(7). Epub 2018 Jun 28.

College of Textiles, Donghua University, No. 2999, Northern Renmin Rd., Songjiang District, Shanghai 201620, China.

Tensile and compressive properties of interlayer and intralayer hybrid composites were investigated in this paper. The tensile modulus and compression modulus of interlayer and intralayer hybrid composites are the same under the same mixed ratio, the tensile strength is much superior to the compression strength, and while the tensile modulus and strength increase along with the carbon fiber content, the compression values change slightly. The influence of stacking structures on the tensile and compressive strengths is opposite to the ratio of T/C (tensile/compression) strength for interlayer hybrid composites, and while the tensile and compression strengths with glass fiber sandwiching carbon fiber can reach the maximum value, the ratio of T/C strength is minimum. For structures with carbon fiber sandwiching glass fiber, or with asymmetric structures, the tensile and compressive strengths are at a low value. For intralayer hybrid structures, while the carbon/glass (C/G) dispersion degree is high, the tensile and compression strengths are low. The experimental tensile and compressive strengths for interlayer and intralayer hybrid composites are greater than the theoretical values, which demonstrates that strength conforms well to the positive hybrid effect. The tensile fracture strain is greater than the compression fracture strain for hybrid composites, with both of them basically maintained at the same level.
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http://dx.doi.org/10.3390/ma11071105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073288PMC
June 2018

Uncertainty evaluation in clinical chemistry, immunoassay, hematology and coagulation analytes using only external quality assessment data.

Clin Chem Lab Med 2018 08;56(9):1447-1457

Department of Clinical Laboratory, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, P.R. China.

Background: Measurement uncertainty (MU) is a parameter associated with the result of a measurement that characterizes its dispersion. We report results for estimating MU following the application of a top-down procedure using only proficiency test data to establish uncertainty levels for various analytes.

Methods: Data were obtained from 142 laboratories participating in the Beijing Center for Clinical Laboratory (BCCL) proficiency testing/external quality assessment (PT/EQA) schemes. The 24-month study included six selected PT shipments to obtain estimates for 50th percentile (median) and 90th percentile MUs and to compare those estimates to usual analytic goals. The number of laboratory participants varied for each trial. The expanded uncertainty (U) was calculated using a cover factor of k=2 for a confidence interval of 95%. All reproducibility, method and laboratory biases came from the PT/EQA data.

Results: The median U (k=2) ranged from 3.2% (plasma sodium, indirect ion selective electrode) to 32.8% (triglycerides, free glycerol blanking) for clinical chemistry analyte means from participants in the same method group. Immunoassay analyte median U results ranged from 11.3% (CA125 tumor marker, Roche) to 33.8% (prostate-specific antigen [PSA], Abbott). The range for median U was 3.5% (red blood cell [RBC], Abx) to 30.3% (fibrinogen [FBG], other) for hematology and coagulation analytes. The MUs for most analytes satisfied quality requirements.

Conclusions: The use of PT/EQA data, when available, provides an effective means for estimating uncertainties associated with quantitative measurements. Thus, medical laboratories can calculate their own MUs. Proficiency testing organizers can provide participants with an additional MU estimate using only EQA data, which may be updated at the end of each survey.
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http://dx.doi.org/10.1515/cclm-2017-1199DOI Listing
August 2018

Flexural Progressive Failure of Carbon/Glass Interlayer and Intralayer Hybrid Composites.

Materials (Basel) 2018 Apr 17;11(4). Epub 2018 Apr 17.

College of Textiles, Donghua University, No. 2999, Northern Renmin Rd, Songjiang District, Shanghai 201620, China.

The flexural progressive failure modes of carbon fiber and glass fiber (C/G) interlayer and intralayer hybrid composites were investigated in this work. Results showed that the bending failure modes for interlayer hybrid composites are determined by the layup structure. Besides, the bending failure is characterized by the compression failure of the upper layer, when carbon fiber tends to distribute in the upper layer, the interlayer hybrid composite fails early, the failure force is characterized by a multi-stage slightly fluctuating decline and the fracture area exhibits a diamond shape. While carbon fiber distributes in the middle or bottom layer, the failure time starts late, and the failure process exhibits one stage sharp force/stress drop, the fracture zone of glass fiber above the carbon layers presents an inverted trapezoid shape, while the fracture of glass fiber below the carbon layers exhibits an inverted triangular shape. With regards to the intralayer hybrid composites, the C/G hybrid ratio plays a dominating role in the bending failure which could be considered as the mixed failures of four structures. The bending failure of intralayer hybrid composites occurs in advance since carbon fiber are located in each layer; the failure process shows a multi-stage fluctuating decline, and the decline slows down as carbon fiber content increases, and the fracture sound release has the characteristics of a low intensity and high frequency for a long time. By contrast, as glass fiber content increases, the bending failure of intralayer composites is featured with a multi-stage cliff decline with a high amplitude and low frequency for a short-time fracture sound release.
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http://dx.doi.org/10.3390/ma11040619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5951503PMC
April 2018

Glutaredoxin-1 Silencing Induces Cell Senescence via p53/p21/p16 Signaling Axis.

J Proteome Res 2018 03 30;17(3):1091-1100. Epub 2018 Jan 30.

MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University , Beijing 100084, China.

Glutaredoxin-1 (Grx1) catalyzes deglutathionylation with glutathione as a cofactor. Accumulating evidence indicates important roles for Grx1 and S-glutathionylation in the aging process; however, further exploration of Grx1-regulated cellular processes is important to understand the functions of Grx1 in aging. In the present study, we constructed stable Grx1 knockdown or overexpression human cell lines. Grx1 silencing significantly decreased the cellular ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) (GSH/GSSG ratio), resulting in excessive reactive oxygen species (ROS) accumulation, whereas Grx1 overexpression decreased cellular ROS levels. Grx1 silencing also increased glutathionylation of DJ-1 and HSP60, contributing to decreased mitochondrial spare respiration capacity and ATP production. We applied quantitative proteomics to identify differentially expressed proteins between Grx1 knockdown and control cells and showed that Grx1 silencing inactivated DNA replication and damage repair pathways. p53 signaling was activated by Grx1 silencing, which inhibited the CDK4-mediated G1-S transition, resulting in G1 phase cell-cycle arrest and cell senescence, a known hallmark of aging. Taken together, our results indicate that Grx1 regulates DNA replication and damage repair processes and is a potential therapeutic target for aging-related diseases.
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http://dx.doi.org/10.1021/acs.jproteome.7b00761DOI Listing
March 2018
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