Publications by authors named "Qingqing Wang"

591 Publications

Single-particle assessment of six different drug-loading strategies for incorporating doxorubicin into small extracellular vesicles.

Anal Bioanal Chem 2022 Aug 10. Epub 2022 Aug 10.

Department of Chemical Biology, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, People's Republic of China.

Extracellular vesicles (EVs) have emerged as an attractive drug delivery system owing to their natural roles in intercellular communication. On account of the large intrinsic heterogeneity of EVs, it is highly desirable to evaluate not only the encapsulation efficiency but also the alteration of biological functionality after the drug-loading process at the single-particle level. However, the nanoscale size of EVs poses a great challenge. Taking advantage of nano-flow cytometry (nFCM) in the multiparameter analysis of single EVs as small as 40 nm, six commonly used drug-loading strategies (coincubation, electroporation, extrusion, freeze-thawing, sonication, and surfactant treatment) were exploited by employing doxorubicin (Dox) as the model drug. Encapsulation ratio, EV concentration, drug content, and membrane proteins of Dox-loaded EVs were measured at the single-particle level. Our data indicated that coincubation and electroporation outperformed other methods with an encapsulation ratio of approximately 45% and a higher Dox content in single EVs. Interestingly, the labeling ratios of membrane proteins indicated that varying degrees of damage to the surface proteins of EVs occurred upon extrusion, freeze-thawing, sonication, and surfactant treatment. Confocal fluorescence microscopy and flow cytometry analysis revealed that Dox-loaded EVs prepared by electroporation induced the strongest apoptosis followed by coincubation. These results correlated well with their cellular uptake rate and fundamentally with the Dox encapsulation efficiency of single EVs. nFCM provides a rapid and sensitive platform for single-particle assessment of drug-loading strategies for incorporating drugs into EVs.
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http://dx.doi.org/10.1007/s00216-022-04248-4DOI Listing
August 2022

Human microRNA-4433 (hsa-miR-4443) Targets 18 Genes to be a Risk Factor of Neurodegenerative Diseases.

Curr Alzheimer Res 2022 Aug 5. Epub 2022 Aug 5.

Xuzhou Medical University, Xuzhou, Jiangsu 221002, China.

Background: Neurodegenerative diseases, such as Alzheimer's disease patients (AD), Huntington's disease (HD) and Parkinson's disease (PD), are common causes of morbidity, mortality, and cognitive impairment in older adults.

Objective: We aimed to understand the transcriptome characteristics of the cortex of neurodegenerative diseases and to provide an insight into the target genes of differently expressed microRNAs in the occurrence and development of neurodegenerative diseases.

Methods: The Limma package of R software was used to analyze GSE33000, GSE157239, GSE64977 and GSE72962 datasets to identify the differentially expressed genes (DEGs) and microRNAs in the cortex of neurodegenerative diseases. Bioinformatics methods, such as GO enrichment analysis, KEGG enrichment analysis and gene interaction network analysis, were used to explore the biological functions of DEGs. Weighted gene co-expression network analysis (WGCNA) was used to cluster DEGs into modules. RNA22, miRDB, miRNet 2.0 and TargetScan7 databases were performed to predict the target genes of microRNAs.

Results: Among 310 Alzheimer's disease (AD) patients, 157 Huntington's disease (HD) patients and 157 non-demented control (Con) individuals, 214 co-DEGs were identified. Those co-DEGs were filtered into 2 different interaction network complexes, representing immune-related genes and synapse-related genes. The WGCNA results identified five modules: yellow, blue, green, turquoise, and brown. Most of the co-DEGs were clustered into the turquoise module and blue module, which respectively regulated synapse-related function and immune-related function. In addition, human microRNA-4433 (hsa-miR-4443), which targets 18 co-DEGs, was the only 1 co-up-regulated microRNA identified in the cortex of neurodegenerative diseases.

Conclusion: 214 DEGs and 5 modules regulate the immune-related and synapse-related function of the cortex in neurodegenerative diseases. Hsa-miR-4443 targets 18 co-DEGs and may be a potential molecular mechanism in neurodegenerative diseases' occurrence and development.
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http://dx.doi.org/10.2174/1567205019666220805120303DOI Listing
August 2022

Particulate matter and ozone might trigger deaths from chronic ischemic heart disease.

Ecotoxicol Environ Saf 2022 Jul 30;242:113931. Epub 2022 Jul 30.

Department of Environment and Health, Jiangsu Provincial Center for Disease Control and Prevention, 172 Jiangsu Road, Nanjing, Jiangsu, 210009, China. Electronic address:

Aims: To study the association between short-term exposure to air pollutants and mortality of Chronic Ischemic Heart Disease (CIHD).

Methods: Using a case-crossover design, we investigated 148,443 CIHD deaths from 2015 to 2020 in Jiangsu Province, China. Exposure to six ambient pollutants, including PM, PM, NO, CO, SO, and O was assessed by extracting daily concentrations from validated 10 km × 10 km pollutant grids at each subject's residential address. A conditional logistic regression approach was used to explore the exposure-response relationship with adjustment for temperature and relative humidity. We calculated the Population Attributable Fractions (PAFs) and the attributable deaths number of CIHD.

Results: An increase of 10 μg/m in PM and PM exposure was associated with a 1.16% (95% CI: 0.85-1.48%) and 1.80% (1.36-2.24%) increase in CIHD mortality, respectively. A threshold value of 123 µg/m was identified for the association between O exposure and CIHD mortality. Controlling for PM, each increase of 10 µg/m in O (>threshold) was statistically significantly associated with a 0.94% (0.19-1.71%) increase in CIHD mortality, however there was no association between NO, SO, CO exposure and CIHD mortality. Reducing PM, PM and O to the WHO air quality guidelines would prevent 6.16% (95% CI: 4.70-7.58%), 4.30% (3.18-5.43%) and 1.29% (0.48-4.20%) of CIHD deaths, respectively. During the warm season, mortality and PAFs of CIHD associated with PM, PM, and O were significantly higher.

Conclusions: Short-term exposure to ambient PM, PM and O might trigger deaths from CIHD. These findings indicate that the premature deaths of CIHD patients can be alleviated by reducing exposure to polluted air.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113931DOI Listing
July 2022

Elevated standardised uptake value of the free wall of the right ventricular myocardium is correlated with enlarged left ventricular end-diastolic volume among patients with heart failure with reduced ejection fraction: A retrospective study.

Hell J Nucl Med 2022 Aug 3. Epub 2022 Aug 3.

Department of Nuclear Medicine, Shanghai Pulmonary Hospital Affiliated to Tongji University, 507 Zhengmin Road, Yangpu District, Shanghai 200433, China.

This study aimed to investigate the relationship between the standardised uptake value of the free wall of the right ventricular myocardium (SUVrv) and left ventricular end-diastolic volume (LVEDV) among patients with heart failure with reduced ejection fraction (HFrEF) with coronary artery disease (CAD). This retrospective study included 50 patients with CAD and HFrEF scheduled for cardiac viability imaging with electrocardiography-gated fluorine-18-fluorodeoxyglucose (F-FDG) positron emission tomography/computed tomography (PET/CT). The maximal SUVrv was measured. The LVEDV and left ventricular ejection fraction (LVEF) were automatically measured using quantitative gated single-photon emission computed tomography. Plasma brain natriuretic peptide (BNP) levels were obtained from medical records.The LVEF (0.24±0.06) was markedly reduced while the LVEDV (201.5±59.9mL) and BNP level (1348.1±1382.9pg/mL) were remarkably elevated. The SUVrv was 3.7±1.7 and was similar between patients with and without diabetes. The SUVrv was significantly positively correlated with the LVEDV and BNP level (r=0.35, 0.45; P=0.01, ≤0.01, respectively) but was unrelated to the LVEF (r=0.11, P=0.44). Herein, SUVrv was elevated and significantly positively correlated with LVEDV and BNP levels among patients with CAD and HFrEF but was unrelated to LVEF and diabetic status.
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http://dx.doi.org/10.1967/s002449912477DOI Listing
August 2022

The Role of Amnioreduction in Emergency Cervical Cerclage with Bulging Membranes: A Retrospective Comparative Study.

Front Surg 2022 12;9:928322. Epub 2022 Jul 12.

Department of Obstetrics and Gynecology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

The purpose of this study was to investigate the role of amnioreduction in patients who underwent emergency cervical cerclage (ECC) with bulging membranes during the second trimester. This retrospective comparative study included 46 singleton pregnant women who had cervical dilation at least 1 cm with bulging membranes beyond the external cervical os and underwent ECC at the Third Affiliated Hospital of Sun Yat-sen University between December 2016 and December 2021. Cases were categorized as amnioreduction group ( = 16) and non-amnioreduction group ( = 30) according to whether amnioreduction was performed prior to ECC. The gestational age and cervical dilation at cerclage, operative time, prolongation of pregnancy, and outcomes of pregnancy were compared between the two groups. All 46 patients underwent successful ECC excepted one case with intraoperative rupture of membrane in non-amnioreduction group. In the amnioreduction group, the cervical dilation at cerclage was larger than that in the non-amnioreduction group (4.5 ± 2.2 vs. 2.2 ± 1.2 cm,  < 0.001), and had more patients with cervical dilation ≥4 cm (50.0% vs. 10.0%,  = 0.004). However, the gestational age at cerclage, operative time, prolongation of pregnancy, gestational age at delivery were not significantly different between the two groups (22.9 ± 2.8 vs. 22.9 ± 3.2 weeks, 31.1 ± 9.2 vs. 27.9 ± 11.4 min, 21.3 ± 21.5 vs. 38.7 ± 40.2 days, 25.9 ± 4.5 vs. 28.4 ± 6.1 weeks;  > 0.05). The rates of delivery ≥28 weeks, ≥32 weeks, and live birth were 20.0% vs. 80.0%, 12.5% vs. 26.7%, 56.3% vs. 66.7% ( > 0.05) in amnioreduction group and non-amnioreduction group, respectively. In conclusion, even in cases with larger cervical dilation, the application of amnioreduction with ECC is possible to get the acceptable pregnancy outcomes. These findings suggested that amnioreduction may be a safe and feasible option to be applied before ECC, especially for those with advanced cervical dilation and bulging membranes.
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http://dx.doi.org/10.3389/fsurg.2022.928322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314748PMC
July 2022

Analysis of Competitive Endogenous RNA Regulatory Network of Exosomal Breast Cancer Based on exoRBase.

Evol Bioinform Online 2022 20;18:11769343221113286. Epub 2022 Jul 20.

Department of Oncology, Xuyi People's Hospital, Huai An, Jiangsu, China.

Objective: To construct a competitive endogenous RNA (ceRNA) regulatory network derived from exosomes of human breast cancer (BC) by using the exoRbase database, to explore the possible pathogenesis of BC, and to develop new targets for future diagnosis and treatment.

Methods: The exosomal gene sequencing data of BC patients and normal controls were downloaded from the exoRbase database, and the expression profiles of exosomal mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) were analyzed by using R language. Use Targetscan and miRanda database to jointly predict and differentially express miRNA (microRNA), miRNA combined with mRNA. The miRcode database was used to predict the miRNA combined with differentially expressed lncRNA, and the starBase database was used to predict the miRNA combined with circRNA in the difference table. The related mRNA, circRNA, lncRNA, and their corresponding miRNA prediction data were imported into Cytoscape software to visualize the ceRNA network. Enrichment analysis and visualization of KEGG were carried out using KOBAS. Hub gene was determined by Cytohubba plug-in.

Results: Forty-two differentially expressed mRNA, 43 differentially expressed circRNA, and 26 differentially expressed lncRNA were screened out. The ceRNA network was constructed by using Cytoscape software, including 19 mRNA nodes, 2 lncRNA nodes, 8 circRNA nodes, and 41 miRNA nodes. KEGG enrichment analysis showed that differentially expressed mRNA in the regulatory network mainly enriched the p53 signaling pathway. Find the key Hub gene PTEN.

Conclusion: The ceRNA regulatory network in blood exosomes of BC patients has been successfully constructed in this study, which provides an exact target for the diagnosis and treatment of BC.
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http://dx.doi.org/10.1177/11769343221113286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309761PMC
July 2022

Bis-Pyridine-Based Organogel with AIE Effect and Sensing Performance towards Hg.

Gels 2022 Jul 25;8(8). Epub 2022 Jul 25.

College of Chemistry and Chemical Engineering and Synthesis Key Laboratory of Xinyang City, Xinyang Normal University, Xinyang 464000, China.

A novel gelator () based on a bis-pyridine derivative was designed and synthesized, which could form stable gels in methanol, ethanol, acetonitrile, ethyl acetate, DMF/HO (4/1, /) and DMSO/HO (4/1, /). The self-assembly process of gelator was studied by field emission scanning electron microscopy (FESEM), UV-vis absorption spectroscopy, fluorescence emission spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray powder diffraction and a water contact angle experiment. Gelator exhibited obvious AIE behavior. On the base of its AIE, the gel of could detect Hg, which resulted in fluorescence quenching and a gel-sol transition. H NMR titration experiments with Hg revealed that the metal coordination interaction induced the fluorescence quenching and the breakdown of the noncovalent interaction in the gel system. This research provides a new molecular mode for designing a functional self-assembly gel system.
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http://dx.doi.org/10.3390/gels8080464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331886PMC
July 2022

Based on abnormal fluctuations in user-side flow simulation analysis of low- and medium-pressure gas pipeline leakage monitoring.

PLoS One 2022 25;17(7):e0270290. Epub 2022 Jul 25.

School of Management, Xi'an University of Architecture and Technology, Xi'an, Shaanxi, China.

Due to the weak monitoring equipment for low- and medium-pressure gas pipelines, it is not easy to identify small flow leaks. The detection methods are mostly traditional manual inspections or night pressure-maintaining leak detection methods, which cannot be automatically monitored and sensed immediately. The abnormal fluctuations in client traffic caused by pipeline leaks studied in this paper can locate and detect leak locations more effectively. This paper analyzes the theoretical formula of leakage location based on flow data and finds out how to use the abnormal fluctuation of user-side flow to detect and locate gas pipeline leakage. First of all, this article uses the simulation software Pipeline Studio to construct the medium and low-pressure pipeline model. On this basis, 6 sets of leakage conditions were designed and simulated dynamically. Finally, the simulation results are analyzed, and the results show that: 1) The advisable monitoring period for monitoring abnormal fluctuations of user-side traffic is 10s. 2) There are two relationships between abnormal flow fluctuations and leakage position. They are: when the leakage point is at the first 40% of the relative distance from the gas source, the disturbance amplitude first increases and then decreases, and at the last 60%, it continues to decrease.; The closer the leak is to the user end, the more significant the abnormal flow fluctuations will be. On the contrary, the smaller the abnormal flow fluctuations will be; 3) No matter where the leakage occurs, the abnormal flow fluctuations in the 2nd and 3rd seconds after the leak occurs tend to be consistent. The proposal of the advisable monitoring period and the relationship between abnormal fluctuations of flow and the location of leakage provides a theoretical basis for the use of abnormal fluctuations of user-side flow for gas pipeline leakage detection and location.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270290PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312409PMC
July 2022

Effects and Prognostic Values of Circadian Genes CSNK1E/GNA11/KLF9/THRAP3 in Kidney Renal Clear Cell Carcinoma via a Comprehensive Analysis.

Bioengineering (Basel) 2022 Jul 11;9(7). Epub 2022 Jul 11.

School of Life Science, Bengbu Medical College, Bengbu 233030, China.

Kidney renal clear cell carcinoma (KIRC) is one of the most prevalent and deadly types of renal cancer in adults. Recent research has identified circadian genes as being involved in the development and progression of KIRC by altering their expression. This study aimed to identify circadian genes that are differentially expressed in KIRC and assess their role in KIRC progression. In KIRC, there were 553 differentially expressed rhythm genes (DERGs), with 300 up-regulated and 253 down-regulated DERGs. Functional enrichment analyses showed that DERGs were greatly enriched in the circadian rhythm and immune response pathways. Survival analyses indicated that higher expression levels of CSNK1E were related to shorter overall survival of KIRC patients, whereas lower expression levels of GNA11, KLF9, and THRAP3 were associated with shorter overall survival of KIRC patients. Through cell assay verification, the mRNA level of CSNK1E was significantly up-regulated, whereas the mRNA levels of GNA11, KLF9, and THRAP3 were dramatically down-regulated in KIRC cells, which were consistent with the bioinformatics analysis of KIRC patient samples. Age, grade, stage, TM classification, and CSNK1E expression were all shown to be high-risk variables, whereas GNA11, KLF9, and THRAP3 expression were found to be low-risk factors in univariate Cox analyses. Multivariate Cox analyses showed that CSNK1E and KLF9 were also independently related to overall survival. Immune infiltration analysis indicated that the proportion of immune cells varied greatly between KIRC tissues and normal tissue, whereas CSNK1E, GNA11, KLF9, and THRAP3 expression levels were substantially linked with the infiltration abundance of immune cells and immunological biomarkers. Moreover, interaction networks between CSNK1E/GNA11/KLF9/THRAP3 and immune genes were constructed to explore the stream connections. The findings could help us better understand the molecular mechanisms of KIRC progression, and CSNK1E/GNA11/KLF9/THRAP3 might be used as molecular targets for chronotherapy in KIRC patients in the near future.
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http://dx.doi.org/10.3390/bioengineering9070306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311602PMC
July 2022

Targeting tumor endothelial hyperglycolysis enhances immunotherapy through remodeling tumor microenvironment.

Acta Pharm Sin B 2022 Apr 22;12(4):1825-1839. Epub 2022 Feb 22.

Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.

Vascular abnormality is a hallmark of most solid tumors and facilitates immune evasion. Targeting the abnormal metabolism of tumor endothelial cells (TECs) may provide an opportunity to improve the outcome of immunotherapy. Here, in comparison to vascular endothelial cells from adjacent peritumoral tissues in patients with colorectal cancer (CRC), TECs presented enhanced glycolysis with higher glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. Then an unbiased screening identified that osimertinib could modify the GAPDH and thus inhibit its activity in TECs. Low-dose osimertinib treatment caused tumor regression with vascular normalization and increased infiltration of immune effector cells in tumor, which was due to the reduced secretion of lactate from TECs by osimertinib through the inhibition of GAPDH. Moreover, osimertinib and anti-PD-1 blockade synergistically retarded tumor growth. This study provides a potential strategy to enhance immunotherapy by targeting the abnormal metabolism of TECs.
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http://dx.doi.org/10.1016/j.apsb.2022.02.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279856PMC
April 2022

Chinese Medicine, Succinum, Ameliorates Cognitive Impairment of Carotid Artery Ligation Rats and Inhibits Apoptosis of HT22 Hippocampal Cells Regulation of the GSK3β/β-Catenin Pathway.

Front Pharmacol 2022 15;13:867477. Epub 2022 Jun 15.

Jiangsu Key Laboratory for High Technology Research of TCM Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nan Jing, China.

Succinum is an organic mineral formed from the resin of ancient coniferous and leguminous plants, which is applied for tranquilizing mood, promoting blood circulation, and removing blood stasis in Chinese medicine. For quite a long time, the modern research of succinum mainly focuses on the study of physical and chemical properties and authenticity identification while few reports on its medicinal mechanism. In current study, we evaluated different solvent extracts of succinum on carotid artery ligation rats mimicking vascular dementia. It was found that ethyl acetate extracts of succinum significantly improved the learning and memory abilities of model rats and inhibited neuronal apoptosis in the hippocampus. On a mice hippocampal neuronal cell line (HT22), ethyl acetate extracts of succinum also exerted better action trend in inhibiting cell apoptosis induced by oxygen glucose deprivation (OGD). By using XAV-939 on both and studies, it was found that ethyl acetate extracts of succinum might exert these functions by regulating the GSK3β/β-catenin pathway. These studies revealed the neuronal function of succinum, which explained the traditional effects of succinum and provided more modern scientific basis for its clinical application.
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http://dx.doi.org/10.3389/fphar.2022.867477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240707PMC
June 2022

Mitochondrial Dynamics and Mitophagy in Cardiometabolic Disease.

Front Cardiovasc Med 2022 17;9:917135. Epub 2022 Jun 17.

Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Mitochondria play a key role in cellular metabolism. Mitochondrial dynamics (fusion and fission) and mitophagy, are critical to mitochondrial function. Fusion allows organelles to share metabolites, proteins, and mitochondrial DNA, promoting complementarity between damaged mitochondria. Fission increases the number of mitochondria to ensure that they are passed on to their offspring during mitosis. Mitophagy is a process of selective removal of excess or damaged mitochondria that helps improve energy metabolism. Cardiometabolic disease is characterized by mitochondrial dysfunction, high production of reactive oxygen species, increased inflammatory response, and low levels of ATP. Cardiometabolic disease is closely related to mitochondrial dynamics and mitophagy. This paper reviewed the mechanisms of mitochondrial dynamics and mitophagy (focus on MFN1, MFN2, OPA1, DRP1, and PINK1 proteins) and their roles in diabetic cardiomyopathy, myocardial infarction, cardiac hypertrophy, heart failure, atherosclerosis, and obesity.
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http://dx.doi.org/10.3389/fcvm.2022.917135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247260PMC
June 2022

Suppression of osteoclast multinucleation via a posttranscriptional regulation-based spatiotemporally selective delivery system.

Sci Adv 2022 07 29;8(26):eabn3333. Epub 2022 Jun 29.

Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang 310016, China.

Redundancy of multinucleated mature osteoclasts, which results from the excessive fusion of mononucleated preosteoclasts (pOCs), leads to osteolytic diseases such as osteoporosis. Unfortunately, the currently available clinical drugs completely inhibit osteoclasts, thus interfering with normal physiological bone turnover. pOC-specific regulation may be more suitable for maintaining bone homeostasis. Here, circBBS9, a previously unidentified circular RNA, was found to exert regulatory effects via the circBBS9/miR-423-3p/Traf6 axis in pOCs. To overcome the long-standing challenge of spatiotemporal RNA delivery to cells, we constructed biomimetic nanoparticles to achieve the pOC-specific targeted delivery of circBBS9. pOC membranes (POCMs) were extracted to camouflage cationic polymer for RNA interference with circBBS9 ([email protected]/shRNA). POCM-NPs endowed the nanocarriers with improved stability, accurate pOC targeting, fusogenic uptake, and reactive oxygen species-responsive release. In summary, our findings may provide an alternative strategy for multinucleated cell-related diseases that involves restriction of mononucleated cell multinucleation through a spatiotemporally selective delivery system.
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http://dx.doi.org/10.1126/sciadv.abn3333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242458PMC
July 2022

A sensitive photoluminescent sensor based on highly charged monoruthenium(II) complexes for dopamine detection.

J Inorg Biochem 2022 Sep 15;234:111902. Epub 2022 Jun 15.

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University, Fuzhou 350116, PR China. Electronic address:

A sensitive and selective photoluminescent sensor based on the highly charged monoruthenium(II) complex was designed to detect dopamine (DA) in aqueous samples. Two novel highly charged cationic ruthenium(II) complexes [Ru(bpy)(bpy-N)]X (bpy = 2,2'-bipyridine, bpy-N = 4,4'-bis[N,N,N-triethyl-(methylamino)]-2,2'-bipyridine, X = [PF] (1a) or Cl (1b) and [Ru(bpy)(bpy-N)]X (X = [PF] (2a) or Cl(2b)) can be assembled with anionic surfactant sodium dodecylbenzene sulfonate (SDBS), leading to an enhancement of photoluminescence intensity. Upon addition of DA to the system, the photoluminescence intensity of the assembled system was quenched due to the energy transfer effect. It exhibited a wide linear range (0.1-50 μM) and low detection limit (10 nM). The sensor demonstrated a high selectivity toward DA, especially in the presence of adrenaline (Adr) and norepinephrine (NE), whose structures are similar to DA in biological systems. With the merits of simple operation, obvious phenomenon and fast response speed, the sensor had a potential application prospect in human urine sample.
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http://dx.doi.org/10.1016/j.jinorgbio.2022.111902DOI Listing
September 2022

Reduction of selenite to selenium nanospheres by Se(IV)-resistant Lactobacillus paralimentarius JZ07.

Food Chem 2022 Nov 2;393:133385. Epub 2022 Jun 2.

College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China. Electronic address:

Elemental selenium nanosphere is considered to exhibit high bioavailability compared to its salts. In this study, a Se(IV)-resistant Lactobacillus paralimentarius strain JZ07 with great selenium biotransformation ability was screened and the red elemental selenium biosynthesized by it was characterized. The results indicated that Se(0) occurred as major accumulated species and the S atom content of the cells increased significantly in the presence of selenite. The reduced amorphous selenium nanospheres (150 to 300 nm in diameter) deposited in the extracellular space of JZ07 and the cells exhibited altered morphology under selenium stress. The macromolecules containing carboxylate bands and amide groups played an important role in Se(IV) bioaccumulation. The findings of present study indicate that JZ07 can be a promising SeNPs producing probiotic LAB and has the potential to be explored as an alternative source of Se supplements for human or animal consumption.
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http://dx.doi.org/10.1016/j.foodchem.2022.133385DOI Listing
November 2022

Development of a Monoclonal Antibody to Pig CD69 Reveals Early Activation of T Cells in Pig after PRRSV and ASFV Infection.

Viruses 2022 06 20;14(6). Epub 2022 Jun 20.

College of Animal Science, Zhejiang University, Hangzhou 310058, China.

The CD69 molecule, as an early activation marker of lymphocytes, is often used to assess the activation of cellular immunity. However, for pigs, an anti-pig CD69 antibody is not yet available for this purpose after infection or vaccination. In this study, a monoclonal antibody (mAb) against pig CD69 was produced by peptide immunization and hybridoma technique. One mAb (5F12) showed good reactivity with pig CD69 that was expressed in transfected-HEK-293T cells and on mitogen-activated porcine peripheral blood mononuclear cells (PBMCs) by indirect immunofluorescence assay and flow cytometry. This mAb did not cross-react with activated lymphocytes from mouse, bovine, and chicken. Epitope mapping showed that the epitope recognized by this mAb was located at amino acid residues 147-161 of pig CD69. By conjugating with fluorochrome, this mAb was used to detect the early activation of lymphocytes in PRRSV- and ASFV-infected pigs by flow cytometry. The results showed that PRRSV infection induced the dominant activation of CD4 T cells in mediastinal lymph nodes and CD8 T cells in the spleen at 14 days post-infection, in terms of CD69 expression. In an experiment on ASFV infection, we found that ASFV infection resulted in the early activation of NK cells, B cells, and distinct T cell subsets with variable magnitude in PBMCs, spleen, and submandibular lymph nodes. Our study revealed an early event of lymphocyte and T cell activation after PRRSV and ASFV infections and provides an important immunological tool for the in-depth analysis of cellular immune response in pigs after infection or vaccination.
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http://dx.doi.org/10.3390/v14061343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231377PMC
June 2022

C1R, CCL2, and TNFRSF1A Genes in Coronavirus Disease-COVID-19 Pathway Serve as Novel Molecular Biomarkers of GBM Prognosis and Immune Infiltration.

Dis Markers 2022 18;2022:8602068. Epub 2022 Jun 18.

School of Life Science, Bengbu Medical College, 233030 Bengbu City, Anhui Province, China.

Glioblastoma multiforme (GBM) is a prevalent intracranial brain tumor associated with a high rate of recurrence and treatment difficulty. The prediction of novel molecular biomarkers through bioinformatics analysis may provide new clues into early detection and eventual treatment of GBM. Here, we used data from the GTEx and TCGA databases to identify 1923 differentially expressed genes (DEGs). GO and KEGG analyses indicated that DEGs were significantly enriched in immune response and coronavirus disease-COVID-19 pathways. Survival analyses revealed a significant correlation between high expression of C1R, CCL2, and TNFRSF1A in the coronavirus disease-COVID-19 pathway and the poor survival in GBM patients. Cell experiments indicated that the mRNA expression levels of C1R, CCL2, and TNFRSF1A in GBM cells were very high. Immune infiltration analysis revealed a significant difference in the proportion of immune cells in tumor and normal tissue, and the expression levels of C1R, CCL2, and TNFRSF1A were associated with immune cell infiltration of GBM. Additionally, the protein-protein interaction networks of C1R, CCL2, and TNFRSF1A involved a total of 65 nodes and 615 edges. These results suggest that C1R, CCL2, and TNFRSF1A may be used as molecular biomarkers of prognosis and immune infiltration in GBM patients in the future.
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http://dx.doi.org/10.1155/2022/8602068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206210PMC
June 2022

Vertically Resolved Aerosol Chemistry in the Low Boundary Layer of Beijing in Summer.

Environ Sci Technol 2022 07 15;56(13):9312-9324. Epub 2022 Jun 15.

State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China.

Air quality in Beijing has been improved significantly in recent years; however, our knowledge of the vertically resolved aerosol chemistry in summer remains poor. Here, we carried out comprehensive measurements of aerosol composition, gaseous species, and aerosol optical properties on a meteorological tower in Beijing in summer and compared with those measured in winter. Our results showed that aerosol liquid water (ALW) contributing approximately 50% of the total mass with higher values aloft played a crucial role in aerosol formation. Particularly, the higher nitrate concentration in city aloft than at the ground level during daytime was mainly due to the enhanced gas-particle partitioning driven by ALW and particle acidity. The vertical profiles of organic aerosol (OA) factors varied more differently in the urban boundary layer. Although the ubiquitous decreases in primary OA with the increase in height were mainly due to the influences of local emissions and vertical convection, the vertical differences in oxygenated OA between summer and winter may be related to the photochemical processing of different biogenic and anthropogenic volatile organic compounds. The single-scattering albedo, brown carbon, and absorption Ångstrom exponent of aerosol particles also presented different vertical profiles between day and night due to the vertical changes in aerosol chemistry.
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http://dx.doi.org/10.1021/acs.est.2c02861DOI Listing
July 2022

Dynamic transcriptome landscape of pulmonary tissues of rats infected with .

Am J Transl Res 2022 15;14(5):3395-3406. Epub 2022 May 15.

School of Basic Medicine, Kunming Medical University Kunming 650504, Yunnan, China.

Paragonimiasis (pulmonary fluke disease) is a foodborne parasitic disease caused by trematode infections. is a characteristic species that was first identified in Yunnan Province of China. No direct evidence has yet proven that can infect humans. However, we previously found that infects and damages rat lung tissues via an unclear mechanism. Here, we infected Sprague Dawley rats with and sequenced their lung transcriptomes at various intervals thereafter. We detected on the surface of rat lung tissues at 7 days post infection. It colonized by attaching and secreting dsRNA and utilized nutrients from the lung tissues for mitosis and meiosis and the dynein arm of lung tissues to develop symmetrical organs. The rats generated different types of immune responses that differed according to the stage of infection. We then analyzed responses to these immune strategies and the genes expressed during each stage of infection. Our findings provide a foundation for developing medical treatments for infection.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185055PMC
May 2022

Comprehensive metabolite quantitative assay based on alternate metabolomics and lipidomics analyses.

Anal Chim Acta 2022 Jul 23;1215:339979. Epub 2022 May 23.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China; Liaoning Province Key Laboratory of Metabolomics, Dalian, 116023, PR China. Electronic address:

Metabolomics-based precision medicine is facing several obstacles including cross-platform data comparison issue and the lack of metabolome benchmark values of healthy population, one of main reasons is the shortage of comprehensive metabolome quantitation methods. Here, we developed an alternate reversed-phase liquid chromatography-mass spectrometry (RPLC-MS) method to quantitatively determine metabolites and lipids. Assisted by a wide set of reference standards and real samples, up to 397 multiple reaction monitoring (MRM) transitions (239 for positive and 158 for negative ion modes) and 1080 MRM transitions (607 for positive and 473 for negative ion modes) were defined respectively in the metabolomic and lipidomic analyses with more than 1000 metabolites and lipids being quantified. Among them, 144 analytes including amines, amino acids, benzenoids, peptides, nucleobases and related, bile acids, carboxylic acids, fatty acids, hormones, indoles and others were absolutely quantified, while carnitines, lyso-phosphatidylcholines, lyso-phosphatidylethanolamines, free fatty acids, sphingomyelins, phosphatidylcholines (PCs), alkyl and alkenyl substituted PCs, phosphatidylethanolamines (PEs), alkyl and alkenyl substituted PEs and triacylglycerols were semiquantified. The developed method was validated to have good analytical characteristics. Analytical results of standard reference material 1950 human plasma had a good agreement with literature data. As a proof of application, this method was used to study serum metabolic pattern changes of patients with hyperuricemia and nonalcoholic fatty liver. This alternate RPLC-MS method for quantitative metabolites and lipids analysis can further be used to provide technology and large-scale data support for precision medicine and life sciences.
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http://dx.doi.org/10.1016/j.aca.2022.339979DOI Listing
July 2022

Effect of Compound Lactic Acid Bacteria Capsules on the Small Intestinal Bacterial Overgrowth in Patients with Depression and Diabetes: A Blinded Randomized Controlled Clinical Trial.

Dis Markers 2022 29;2022:6721695. Epub 2022 May 29.

Digestive Department, Cangzhou Central Hospital, Cangzhou, China.

Objective: The objective is to explore the clinical effect of compound lactic acid bacteria capsules on the small intestinal bacterial overgrowth (SIBO) in patients with depression and diabetes.

Methods: From January 2020 to January 2021, 60 SIBO patients with depression and diabetes in our hospital were selected and randomized into observation group (compound lactic acid bacteria capsules combined with escitalopram) and control group (Escitalopram) according to the odd and even numbers, 30 cases in each group. The two groups were compared in terms of SAS, SDS, levels of inflammatory factors, immune function, fasting plasma glucose (FPG), treatment effect, and the incidence of adverse reactions.

Results: Both self-rating anxiety scale (SAS) and self-rating depression scale (SDS) scores in both groups showed a decline after treatment ( < 0.05), and the reduction was more significant in the observation group ( = 10.047, 17.862, all ≤ 0.001). Both IL-2 and TNF- in both groups showed a decline after treatment ( < 0.05), and the reduction was more greater in the observation group in relative to the control group ( < 0.05). CD3+ and CD+4 in both groups showed an increase after treatment ( < 0.05), and the increase was more greater in the observation group as compared to the control group ( < 0.05). After treatment, the FPG levels of patients in both groups showed a decline ( < 0.05), and the reduction of FPG levels was more significant in the observation group than that in the control group ( = 3.948, ≤ 0.001). The control group experienced a remarkably higher incidence of adverse reactions.

Conclusion: The compound lactic acid bacteria capsule is a boon for SIBO patients with depression and diabetes. It can mitigate depression symptoms, improve immune function, reduce the level of inflammatory factors, and lower the FPG levels, along with fewer adverse reactions and robust effects.
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http://dx.doi.org/10.1155/2022/6721695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168214PMC
June 2022

Scutellarin enhances anti-tumor immune responses by reducing TNFR2-expressing CD4Foxp3 regulatory T cells.

Biomed Pharmacother 2022 Jul 27;151:113187. Epub 2022 May 27.

Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, 999078, Macao Special Administrative Region of China, China; Department of Pharmaceutical Sciences, Faculty of Health Sciences, University of Macau, 999078, Macao Special Administrative Region of China, China; MoE Frontiers Science Center for Precision Oncology, University of Macau, 999078, Macao Special Administrative Region of China, China; Guangdong-Hong Kong-Macau Joint Lab on Chinese Medicine and Immune Disease Research, University of Macau, 999078, Macao Special Administrative Region of China, China. Electronic address:

One characteristic of tumor-associated CD4Foxp3 regulatory T cells (Tregs) is the high expression of tumor necrosis factor receptor type II (TNFR2), a receptor that mediates the decisive effect of tumor necrosis factor (TNF) in the activation and expansion of Tregs. There is increasing evidence that inhibition of TNFR2 can enhance anti-tumor immune responses. Therefore, we screened Chinese herbal extracts for their capacity to block TNF-TNFR2 interaction. The results showed that the treatment with a Chinese herb extract could inhibit TNFR2-induced biological responses in vitro, including the proliferation of TNFR2 Tregs. Our subsequent study led to the identification of flavonoid compound scutellarin was responsible for the activity. Our results showed that scutellarin is able to disrupt the interaction of TNF-TNFR2 and inhibited the phosphorylation of p38 MAPK, a down-stream signaling component of TNFR2. Importantly, in vivo scutellarin treatment markedly enhanced the efficacy of tumor immunotherapy with CpG oligodeoxynucleotide in mouse CT26 colon cancer model. This effect of scutellarin was associated with the reduction of the number of tumor-infiltrating TNFR2-expressing Tregs and increased tumor infiltration of interferon-γ-producing CD8 T cells. Our result also suggests that scutellarin or its analogs may be used as an adjuvant to enhance the anti-tumor effect of immunotherapeutic agent by eliminating TNFR2 Treg activity.
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http://dx.doi.org/10.1016/j.biopha.2022.113187DOI Listing
July 2022

A single-cell map of dynamic chromatin landscapes of immune cells in renal cell carcinoma.

Nat Cancer 2022 Jul 6;3(7):885-898. Epub 2022 Jun 6.

Regeneron Pharmaceuticals, Tarrytown, NY, USA.

A complete chart of the chromatin regulatory elements of immune cells in patients with cancer and their dynamic behavior is necessary to understand the developmental fates and guide therapeutic strategies. Here, we map the single-cell chromatin landscape of immune cells from blood, normal tumor-adjacent kidney tissue and malignant tissue from patients with early-stage clear cell renal cell carcinoma (ccRCC). We catalog the T cell states dictated by tissue-specific and developmental-stage-specific chromatin accessibility patterns, infer key chromatin regulators and observe rewiring of regulatory networks in the progression to dysfunction in CD8 T cells. Unexpectedly, among the transcription factors orchestrating the path to dysfunction, NF-κB is associated with a pro-apoptotic program in late stages of dysfunction in tumor-infiltrating CD8 T cells. Importantly, this epigenomic profiling stratified ccRCC patients based on a NF-κB-driven pro-apoptotic signature. This study provides a rich resource for understanding the functional states and regulatory dynamics of immune cells in ccRCC.
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http://dx.doi.org/10.1038/s43018-022-00391-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325682PMC
July 2022

Transcriptomic signatures associated with autoimmune thyroiditis in papillary thyroid carcinoma and cancer immunotherapy-induced thyroid dysfunction.

Comput Struct Biotechnol J 2022 16;20:2391-2401. Epub 2022 May 16.

Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Radiation Oncology, Peking University Cancer Hospital & Institute, Beijing, China.

Up to 20% of patients treated with anti-PD-1/PD-L1 inhibitors suffered from thyroid dysfunctions, yet the mediators associated with their occurrence remain unclear. The increasing coincidence of papillary thyroid carcinoma (PTC) with Hashimoto thyroiditis (HT) and the high vulnerability of thyroid to immunotherapy motivated us to discover the similarities and their underlying transcriptomic basis. Clinical characteristics analysis of 468 PTC patients from two independent cohorts and meta-analysis of 22,155 PTC patients unveiled a strong negative association between HT and recurrence in PTC patients. Transcriptome analysis of both cohorts showed PTC patients with HT were enriched in macrophages, CD8 and CD4 cytotoxic T cells, which was further validated by single-cell transcriptome analysis of 17,438 cells from PTC patients, and CD8 T cells were correlated with disease-free survival of PTC patients. In both cohorts and single-cell dataset, elevated expression of PD-1-related genes was observed in the HT group, and appeared to be a target for enhancing the activation of CD8 T cells. Correlation analysis of 3,318 thyroid adverse events from 39,123 patients across 24 tumor types and molecular signatures demonstrated similar signatures associated with autoimmune thyroiditis in PTC and thyroid immune-related adverse events (irAEs), and several multi-omics signatures, including signatures of CD8A and CD8 T cells, showed positive associations with the odds ratio of thyroid irAEs. Our results unveil shared molecular signatures underlying thyroid dysfunction between patients receiving immunotherapies and PTC patients suffering from HT, which may shed light on managing the adverse events during cancer immunotherapy.
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http://dx.doi.org/10.1016/j.csbj.2022.05.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125670PMC
May 2022

A Cohort Study on the Comparison of Complications, Short-Term Efficacy, and Quality of Life between Thoracoscopic Surgery and Traditional Surgery in the Treatment of Rib Fractures.

Contrast Media Mol Imaging 2022 18;2022:2079098. Epub 2022 May 18.

Department of Thoracic Surgery, Shanghai Pudong Hospital Fudan University Pudong Medical Center, Shanghai 201399, China.

Objective: A case-control study was conducted, to assess the complications, short-term effectiveness, and quality of life of video-assisted thoracoscopic surgery with conventional surgery in the treatment of rib fractures.

Methods: From February 2018 to April 2021, 100 patients with rib fractures who required surgical treatment at the hospital were selected. Patients were randomly divided into control and study groups. The study group received thoracoscopy-assisted rib internal fixation, and the control group received traditional open reduction and internal fixation for rib fractures. The treatment effect, postoperative complication rate, surgery-related indicators, stress response, blood gas indicators, VAS (visual analog scale) pain score, and SF-36 quality of life score were compared between the two groups.

Results: The total effective rate of the study group was higher than that of the control group, and the difference was statistically significant ( < 0.05). The postoperative complications in the study group were significantly lower than those in the control group ( -5.317; < 0.05), but there was no significant difference in hospitalization costs between the two groups ( > 0.05). The operation time, intraoperative blood loss, incision length, drainage tube placement time, postoperative activity time, and hospital stay in the study group were significantly lower than those in the control group. The SF-36 score and VAS score in the study group were higher than those in the control group ( < 0.05). Compared with the two groups after the operation, the levels of PaO2, SaO2, and PaO2/FiO2 in the study group were significantly higher than those in the control group ( < 0.05). Before surgery, there was no significant difference in stress response indicators such as cortisol, blood sugar, and C-reactive protein between the two groups ( > 0.05), but there was no significant difference in stress response indicators after surgery ( > 0.05). Cortisol, blood sugar, C-reactive protein, and other indicators were increased in both groups, but compared with the control group, the study group had decreased postoperative cortisol, blood sugar, C-reactive protein, and other stress response indicators ( < 0.05).

Conclusion: There is a significant difference between thoracoscopic surgery and traditional surgery in the treatment of rib fractures. The probability of postoperative complications of thoracoscopic surgery is lower, and the operation time, intraoperative blood loss, and incision length are better. The pain of patients before and after the operation is significantly reduced, the quality of life is improved greatly, and the stress response is weak.
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http://dx.doi.org/10.1155/2022/2079098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132641PMC
June 2022

Synergistic simultaneous endogenous partial denitrification/anammox (EPDA) and denitrifying dephosphatation for advanced nitrogen and phosphorus removal in a complete biofilm system.

Bioresour Technol 2022 Aug 26;358:127378. Epub 2022 May 26.

Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province, School of Ecological and Environmental Science, Hainan University, Haikou 570228, China. Electronic address:

To achieve simultaneous biological nitrogen and phosphorus removal from municipal wastewater, the endogenous partial denitrification/anammox (EPDA) was combined with denitrifying dephosphatation in a complete biofilm reactor. Advanced nitrogen and phosphorus removal were achieved with effluent total nitrogen (TN) and PO-P concentrations of 7.77 ± 0.33 mg/L and 0.35 ± 0.10 mg/L, respectively. Anammox took a major role in the system, accounting for 76 ± 7% of nitrogen removal. 16S rRNA high-throughput sequencing results showed that the anammox bacteria co-existed with the denitrifying glycogen accumulating organisms (DGAOs) and the denitrifying phosphorus accumulating organisms (DPAOs). Anammox bacteria were mainly distributed in the inner layer, while DGAOs and DPAOs existed in the outer layer of EPDA biofilms. Furthermore, based on the EPDA biofilm system, a promising advanced nitrogen and phosphorus removal process was suggested to achieve lower requirements for energy and reagent consumption.
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http://dx.doi.org/10.1016/j.biortech.2022.127378DOI Listing
August 2022

High Efficacy Combined Microneedles Array with Methotrexate Nanocrystals for Effective Anti-Rheumatoid Arthritis.

Int J Nanomedicine 2022 24;17:2397-2412. Epub 2022 May 24.

School of Pharmacy, Bengbu Medical College, Bengbu, Anhui Province, 233030, People's Republic of China.

Introduction: Methotrexate (MTX) is the first-line drug for the treatment of rheumatoid arthritis (RA) in several countries. However, MTX has an extremely low solubility in water, and the side effects caused by its delivery mode restrict its curative effect. In this study, we designed a dissolving microneedles array (DMNA) containing MTX nanocrystals (MTX-NCs) ([email protected]) to improve the treatment of RA. DMNA-based drug delivery combines the advantages of patient compliance with the use of transdermal drug delivery systems and high-efficiency injection administration; thus, it can mitigate the side effects that result from current administration routes. Carrier-free and surfactant-free MTX-NCs were prepared to overcome bioavailability limitations and poor drug loading problems.

Methods: The MTX-NCs prepared by reverse solvent precipitation method was encapsulated in the DMNA. The morphology, mechanical properties, safety, stability and in vivo dissolution were evaluated, and its pharmacodynamic characteristics were assessed in a rat model of RA.

Results: The particle size of the MTX-NCs was 148.1 ± 10.1 nm. The [email protected] were found to be rigid enough to penetrate the skin and deliver the drug successfully. The results indicated effective skin recovery after removal of the DMNA. It was found that the [email protected] significantly reduced foot swelling in the rats and regulated the balance in the levels of related cytokines. It also reduced pathological damage to the synovium, joint, and cartilage, and effectively alleviated organ injury in the rats.

Conclusion: Transdermal administration of [email protected] may be an effective approach for treating RA.
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http://dx.doi.org/10.2147/IJN.S365523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148202PMC
June 2022

Primary hepatic neuroendocrine carcinoma with colon adenoma: A case report with literature review.

Int J Surg Case Rep 2022 Jun 10;95:107176. Epub 2022 May 10.

Department of General Surgery, The First Hospital of Jiaxing (The First Affiliated Hospital of Jiaxing University), Jiaxing, Zhejiang 314001, PR China.

Introduction And Importance: Primary hepatic neuroendocrine tumors (PHNETs) are extremely rare, and the clinical symptoms, test results, and imaging characteristics are nonspecific in most patients; thus, it is difficult to differentiate from other liver masses before surgery. Histopathology and immunohistochemistry are the main basis for the diagnosis. PHNETs and colon tumors co-occur in a patient and are non-homologous, as reported in the English-language literature for the first time.

Case Presentation: We present a case of a 60-year-old woman with right hepatic lobe mass accidentally discovered on abdominal ultrasonography during a routine examination. Preoperative liver contrast-enhanced computed tomography suggested hepatocellular carcinoma; then, surgery were performed. Pathological results revealed a Grade 2 neuroendocrine tumor of the liver. In search of the primary tumor, upper and lower endoscopy of the GI tract was performed and revealed a mass in the ascending colon. Ascending colon cancer was considered; then, laparoscopic right hemicolectomy was performed. Pathological results suggested tubular villous adenoma of the ascending colon. The final diagnosis was not colon cancer with liver metastases but was PHNETs with colon adenoma.

Clinical Discussion: PHNETs are rare cancers that are difficult to diagnose, requiring not only differentiation from other liver masses but also exclusion of metastases from extrahepatic sources. The pathological results play an important in making an accurate diagnosis.

Conclusion: Pathology, postoperative follow-up, and comprehensive imaging examinations are powerful tools in the diagnosis of PHNETs. Currently, surgery is the best treatment to achieve a potential cure and prolong the patient's survival.
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http://dx.doi.org/10.1016/j.ijscr.2022.107176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157444PMC
June 2022

Corrigendum: The Effects of rs405509 on ε Non-carriers in Non-demented Aging.

Front Neurosci 2022 10;16:928289. Epub 2022 May 10.

Department of Neurology, The Affiliated Hospital of Qingdao University, Qingdao, China.

[This corrects the article DOI: 10.3389/fnins.2021.677823.].
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http://dx.doi.org/10.3389/fnins.2022.928289DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9128587PMC
May 2022

Temporal variation of the coupling relationship between methanogens and biogeochemical process in soil-microbes-rice system.

Chemosphere 2022 Sep 23;303(Pt 1):135099. Epub 2022 May 23.

Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province/Center for Eco-Environmental Restoration Engineering of Hainan Province/College of Ecology & Environment/State Key Laboratory of Marine Resource Utilization in South China Sea/Key Laboratory for Environmental Toxicology of Haikou, Haikou, Hainan University, Hainan, 570228, China.

Microbial community were most resilient option for methane associated mitigation strategies. Biogas slurry provides plant nutrition and affects microbial community. However, little is known about the changes of the functional guilds (methanogen and methanotroph) in the geochemical context after addition biogas slurry. For this purpose, a pot experiment was conducted. Six treatment groups were included in this study, four with biogas slurry: water ratio (1:4, T02; 2:3, T04; 3:2, T06; 4:1, T08), one with a chemical fertilizer (F), and a control (CK). The effective tiller and biomass significantly increased by 1.9 times and 2.1 times in T02 relative to CK. The relative abundance of Bacteroidetes in the biogas slurry treatments was 31.5%, while that in CK was 11.4%. The dominant methanogens in CK, F and treatments were different at heading and mature stages. CK and F were hydrogenotrophs with relative abundance of 0.09% and 0.06%, and the treatment group was acetotrophs with mean value of 1.21% at heading stage. Compared with CK, the number of methanotrophs in the treatments at heading stage increased by 4.1 times, while that at mature increased by 10.3 times. The methanogenic community in the treatments may be shaped by the amount of biogas slurry applied rather than by biogeochemical processes at heading stage. Nevertheless, there may be existed synergistic interaction in the soil-microbes-rice system at mature stage. These findings may provide a better understanding of regulating soil respiration in agricultural land.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135099DOI Listing
September 2022
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