Publications by authors named "Qingfu Wang"

39 Publications

Is Induced by Agricultural and Forestry Residues and Exhibits Allelic Expression Bias in .

Front Microbiol 2022 30;13:890686. Epub 2022 Jun 30.

Guangdong Engineering Laboratory of Biomass High-Value Utilization, Guangdong Plant Fiber Comprehensive Utilization Engineering Technology Research and Development Center, Guangzhou Key Laboratory of Biomass Comprehensive Utilization, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou, China.

has a wide carbon spectrum, while the expression profile of key genes relevant to carbon metabolism on different carbon sources has been seldom studied. Here, the transcriptomes of mycelia cultured on each of 19 carbon sources were conducted. In comparison with glucose, 16 to 1,006 genes were upregulated and 7 to 1,865 genes were downregulated. Significant gene expression dynamics and induced activity were observed in laccase genes when using agricultural and forestry residues (AFRs) as solo carbon sources. Furthermore, study of laccase gene family in two haploids of GL0102 was conducted. Totally, 15 and 16 laccase genes were identified in GL0102_53 and GL0102_8, respectively, among which 15 pairs were allelic genes. Gene structures were conserved between allelic laccase genes, while sequence variations (most were SNPs) existed. Nine laccase genes rarely expressed on all the tested carbon sources, while the other seven genes showed high expression level on AFRs, especially and , which showed 5- to 1,149-fold and 4- to 94-fold upregulation in mycelia cultured for 5 days, respectively. The expression of was consistently higher than that of on all the carbon sources except XM, exhibiting a case of allelic expression bias. A total of 47 SNPs and 3 insertions/deletions were observed between promoters of and , which lead to differences in predicted binding sites of zinc fingers. These results provide scientific data for understanding the gene expression profile and regulatory role on different carbon sources and may support further functional research of laccase.
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http://dx.doi.org/10.3389/fmicb.2022.890686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279560PMC
June 2022

Synergistic Double Cross-Linked Dynamic Network of Epoxidized Natural Rubber/Glycinamide Modified Polyacrylic Acid for Silicon Anode in Lithium Ion Battery: High Peel Strength and Super Cycle Stability.

ACS Appl Mater Interfaces 2022 Jul 14. Epub 2022 Jul 14.

Qingdao University of Science and Technology, Qingdao 266045, China.

Silicon (Si), a high-capacity lithium-ion battery anode material, has aroused wide attention. Its further practical application has been limited by its huge volume change during the cycle. To reduce this defect, the double cross-linked product of glycinamide hydrochloride modified poly(acrylic acid) (PAG) and epoxidized natural rubber (ENR) was developed as a water-based binder to obtain sufficient elasticity and a sufficiently strong adhesive force. Due to the double cross-linked structures in the system, the binder was enabled to effectively disperse and transfer the stress generated by the volume expansion of the Si particles and keep the integrity of the electrode during the cycle, thus obtaining excellent cycle performance. When the current density was 1 A g, PE55 (PAG: ENR = 1:1 cross-linked polymer) electrode still achieved a specific capacity of 2322.2 mAh g after 100 cycles of constant current charge and discharge, and PE55 binder exhibited excellent bonding properties (4.45 N) and mechanical properties (stress: 5.51 MPa, strain: 87.4%). The comparison of poly(acrylic acid) (PAA) electrodes suggests that the introduction of elastic polymer and the construction of double cross-linked structures can increase the stability of Si anodes.
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http://dx.doi.org/10.1021/acsami.2c08038DOI Listing
July 2022

Cyclodextrin-mediated conjugation of macrophage and liposomes for treatment of atherosclerosis.

J Control Release 2022 Jul 4;349:2-15. Epub 2022 Jul 4.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao 999078, China; Department of Pharmaceutical Sciences, Faculty of Health Sciences, University of Macau, Taipa, Macao 999078, China. Electronic address:

Current pharmacological treatments of atherosclerosis often target either cholesterol control or inflammation management, to inhibit atherosclerotic progression, but cannot lead to direct plaque lysis and atherosclerotic regression, partly due to the poor accumulation of medicine in the atherosclerotic plaques. Due to enhanced macrophage recruitment during atheromatous plaque progression, a macrophage-liposome conjugate was facilely constructed for targeted anti-atherosclerosis therapy via synergistic plaque lysis and inflammation alleviation. Endogenous macrophage is utilized as drug-transporting cell, upon membrane-modification with a β-cyclodextrin (β-CD) derivative to form β-CD decorated macrophage (CD-MP). Adamantane (ADA) modified quercetin (QT)-loaded liposome (QT-NP), can be conjugated to CD-MP via host-guest interactions between β-CD and ADA to form macrophage-liposome conjugate (MP-QT-NP). Thus, macrophage carries liposome "hand-in-hand" to significantly increase the accumulation of anchored QT-NP in the aorta plaque in response to the plaque inflammation. In addition to anti-inflammation effects of QT, MP-QT-NP efficiently regresses atherosclerotic plaques from both murine aorta and human carotid arteries via CD-MP mediated cholesterol efflux, due to the binding of cholesterol by excess membrane β-CD. Transcriptome analysis of atherosclerotic murine aorta and human carotid tissues reveal that MP-QT-NP may activate NRF2 pathway to inhibit plaque inflammation, and simultaneously upregulate liver X receptor to promote cholesterol efflux.
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http://dx.doi.org/10.1016/j.jconrel.2022.06.053DOI Listing
July 2022

Annexin V-Modified Platelet-Biomimetic Nanomedicine for Targeted Therapy of Acute Ischemic Stroke.

Adv Healthc Mater 2022 Jun 16:e2200416. Epub 2022 Jun 16.

Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Taipa, Macau SAR, 999078, P. R. China.

Thromboembolic stroke is typically characterized by the activation of platelets, resulting in thrombus in the cerebral vascular system, leading to high morbidity and mortality globally. Intravenous thrombolysis by tissue plasminogen activator (tPA) administration within 4.5 h from the onset of symptoms is providing a standard therapeutic strategy for ischemic stroke, but this reagent simultaneously shows potential serious adverse effects, e.g., hemorrhagic transformation. Herein, a novel delivery platform based on Annexin V and platelet membrane is developed for tPA (APLT-PA) to enhance targeting efficiency, therapeutic effects, and reduce the risk of intracerebral hemorrhage in acute ischemic stroke. After preparation by extrusion of platelet membrane and subsequent insertion of Annexin V to liposomes, APLT-PA exhibits a high targeting efficiency to activated platelet in vitro and thrombosis site in vivo, due to the binding to phosphatidylserine (PS) and activated platelet membrane proteins. One dose of APLT-PA leads to obvious thrombolysis and significant improvement of neurological function within 7 days in mice with photochemically induced acute ischemic stroke. This study provides a novel, safe platelet-biomimetic nanomedicine for precise thrombolytic treatment of acute ischemic stroke, and offers new theories for the design and exploitation of cell-mimetic nanomedicine for diverse biomedical applications.
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http://dx.doi.org/10.1002/adhm.202200416DOI Listing
June 2022

Epitaxial Electrocrystallization of Magnesium Synergy of Magnesiophilic Interface, Lattice Matching, and Electrostatic Confinement.

ACS Nano 2022 Jun 13. Epub 2022 Jun 13.

Qingdao Industrial Energy Storage Research Institute, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, People's Republic of China.

Rechargeable magnesium batteries are particularly advantageous for renewable energy storage systems. However, the inhomogeneous Mg electrodeposits greatly shorten their cycle life under practical conditions. Herein, the epitaxial electrocrystallization of Mg on a three-dimensional magnesiophilic host is implemented the synergy of a magnesiophilic interface, lattice matching, and electrostatic confinement effects. The vertically aligned nickel hydroxide nanosheet arrays grown on carbon cloth (abbreviated as "Ni(OH)@CC") have been delicately designed, which satisfy the essential prerequisite of a low lattice geometrical misfit with Mg (about 2.8%) to realize epitaxial electrocrystallization. Simultaneously, the ionic crystal nature of Ni(OH) displays a periodic and hillock-like electrostatic potential field over its exposed facets, which can precisely capture and confine the reduced Mg species onto the local electron-enriched sites at the atomic level. The Ni(OH)@CC substrate undergoes sequential Mg-ion intercalation, underpotential deposition, and electrocrystallization processes, during which the uniform, lamellar Mg electrodeposits with a locked crystallographic orientation are formed. Under practical conditions (10 mA cm and 10 mAh cm), the Ni(OH)@CC substrate exhibits stable Mg stripping/plating cycle performances over 600 h, 2 orders of magnitude longer than those of the pristine copper foil and carbon cloth substrates.
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http://dx.doi.org/10.1021/acsnano.2c04135DOI Listing
June 2022

Naringin in the repair of knee cartilage injury via the TGF-β/ALK5/Smad2/3 signal transduction pathway combined with an acellular dermal matrix.

J Orthop Translat 2022 Jan 6;32:1-11. Epub 2021 Aug 6.

Orthopedics Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Objective: Based on the expression changes in the TGF-β/ALK5/Smad2/3 signal transduction pathway, the repair of cartilage injury in the rabbit knee joint was investigated and evaluated by oral administration of naringin in combination with acellular dermal matrix implantation.

Methods: First, twenty New Zealand white rabbits were randomly divided into five groups: a sham operation group (Sham group), a model group (Mod group), a naringin group (Nar group), an acellular dermal matrix group (ADM group), a naringin ​+ ​acellular dermal matrix group (Nar/ADM group). After the 12th week, the repaired tissues were assessed for histomorphology and repair content of the repaired site by observing the morphological characteristics of articular cartilage. The International Cartilage Repair Society (ICRS)'s macroscopic evaluation of the cartilage repair scale and the quantitative scoring repair effect of the modified O'Driscoll grading system were used as evaluation criteria. In addition, the structure of the rabbit knee joint was evaluated by micro-CT scan, histological staining (H & E staining, Alcian blue staining, Safranin-O staining) and immunohistochemical staining (TGF-β2 immunostaining, TGF-β3 immunostaining, Sox-9 immunostaining).

Results: ① The observation of the repair morphology of joint defect tissues showed that the repair effects of the Nar and ADM groups were better than that of the Mod group, and the repair effect of Nar/ADM group was the best ( < 0.05). ② Quantitative scoring of joint defect tissue showed that the Nar/ADM group had the best repair efficacy in the quantitative scores of the above two scales compared with the other groups ( < 0.05). ③ Micro-CT scan showed that the ADM group had obvious repair of the defect structure, while the ADM/Nar group had blurred repair boundaries, and the layers of cartilage and subchondral bone were clear. ④ Histological staining (H & E staining, Alcian blue stain, Safranin-O staining) showed that the ADM group had a better effect on the repair of joint structure at the joint defect, the Nar group had a better effect on the repair of cartilage quality at the joint defect, and the ADM/Nar group had satisfactory results in both of the above aspects. ⑤ Immunohistochemical staining (TGF-β2 immunostaining, TGF-β3 immunostaining, Sox-9 immunostaining) revealed that the Nar group showed more abundant expression of the above proteins in articular cartilage defects than the Mod and ADM groups and that the Nar/ADM groups showed extensive TGF-β2, TGF-β3 and Sox-9 protein expression, with uniform expression and smooth distribution.

Conclusions: Oral administration of naringin, the active ingredient of Rhizoma Drynariae, combined with acellular dermal matrix can achieve better repair effects in both joint structure repair and cartilage quality repair at the defect site when repairing cartilage defects in rabbit knees, and the generation of this effect may be caused by the activation of the TGF-β/ALK5/Smad2/3 signal transduction pathway by naringin, resulting in the increased expression of TGF-β2, TGF-β3, and Sox-9 in cartilage defects.

The Translational Potential Of This Article: Naringin combined with acellular dermal matrix can facilitate the repair of osteochondral defects and has potential for application in osteochondral tissue engineering.
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http://dx.doi.org/10.1016/j.jot.2021.06.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072805PMC
January 2022

In vivo hitchhiking of immune cells by intracellular self-assembly of bacteria-mimetic nanomedicine for targeted therapy of melanoma.

Sci Adv 2022 05 11;8(19):eabn1805. Epub 2022 May 11.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau 999078, China.

Cell-based drug carriers are mostly prepared in vitro, which may negatively affect the physiological functions of cells, and induce possible immune rejections when applied to different individuals. In addition, the immunosuppressive tumor microenvironment limits immune cell-mediated delivery. Here, we report an in vivo strategy to construct cell-based nanomedicine carriers, where bacteria-mimetic gold nanoparticles (GNPs) are intravenously injected, selectively phagocytosed by phagocytic immune cells, and subsequently self-assemble into sizable intracellular aggregates via host-guest interactions. The intracellular aggregates minimize exocytosis of GNPs from immune cells and activate the photothermal property via plasmonic coupling effects. Phagocytic immune cells carry the intracellular GNP aggregates to melanoma tissue via inflammatory tropism. Moreover, an initial photothermal treatment (PTT) of the tumor induces tumor damage that subsequently provides positive feedback to recruit more immune cell-based carriers for enhanced targeting efficiency. The optimized secondary PTT notably improves antitumor immunotherapy, further strengthened by immune checkpoint blockade.
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http://dx.doi.org/10.1126/sciadv.abn1805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094661PMC
May 2022

[Study on homeostasis and circadian rhythm of attention performance of different chronotypes in sleep deprivation].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2022 Apr;39(2):248-256

School of Safety Science and Engineering, Civil Aviation University of China, Tianjin 300300, P. R. China.

Difference of chronotypes makes influence to cognitive performance of individuals in routine duties. In this paper, 55 subjects with different chronotypes were subjected to continuous sleep deprivation for 30 h by using the constant routine protocol, during which core body temperature was measured continuously, and subjective sleepiness self-rating and the performance of selective attention were measured hourly. The results showed that the phase difference of core body temperature has no significant difference, yet the amplitude and term difference among the three chronotypes are significant. There was an advance in phase between subjective sleepiness self-rating and core body temperature, and the self-rating sleepiness of evening type came the latest, and the self-rating sleepiness of morning type dissipated the fastest. The response time of selective attention showed a 2 h phase delay with subjective sleepiness self-rating. And the analysis of core body temperature showed that the later the chronotype was, the greater the phase delay was. The correct rate of selective attention of different chronotypes were inconsistent with delay of subjective sleepiness self-rating and core body temperature. We provide reference for industry, aviation, military, medical and other fields to make a more scientific scheduling/ shifting based on cognitive performance characteristics of different chronotypes.
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http://dx.doi.org/10.7507/1001-5515.202110083DOI Listing
April 2022

Effect of hydroxyl on antioxidant properties of 2,3-dihydro-3,5-dihydroxy-6-methyl-4-pyran-4-one to scavenge free radicals.

RSC Adv 2021 Oct 25;11(55):34456-34461. Epub 2021 Oct 25.

Technology Center, China Tobacco Henan Industrial Co., Ltd. Zhengzhou Henan 450016 China

It is well known that 2,3-dihydro-3,5-dihydroxy-6-methyl-4-pyran-4-one (DDMP) is usually formed in the Maillard reaction and it contributes to the antioxidant properties of Maillard reaction intermediates. A series of hydroxyl group protected DDMP derivatives were synthesized to further understand the source of antioxidant activity. Antioxidant abilities of the DDMP derivatives were evaluated by scavenging the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) cationic radical (ABTS˙), 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH), and galvinoxyl radical, respectively. It was found that the introduction of protecting groups to the free hydroxyl groups of DDMP decreases their reducing abilities. In particular, the hydroxyl group at the olefin position exhibited a remarkable impact on the antioxidant activity of DDMP, indicating that the unstable enol structure in the DDMP moiety is the key factor for its antioxidant activity.
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http://dx.doi.org/10.1039/d1ra06317kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042674PMC
October 2021

Enhancement of Rebaudioside M Production by Structure-Guided Engineering of Glycosyltransferase UGT76G1.

J Agric Food Chem 2022 Apr 13;70(16):5088-5094. Epub 2022 Apr 13.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, P. R. China.

Owing to zero-calorie and advanced organoleptic properties similar to sucrose, the plant-derived rebaudioside M (Reb M) has been considered as a next generation sweetener. However, a low content of Reb M in Bertoni and low enzymatic activity of UGT76G1, which is an uridine diphosphate glucose (UDPG)-dependent glycosyltransferase with the ability to glycosylate rebaudioside D (Reb D) to produce Reb M through the formation of β-1,3 glycosidic bond, restrict its commercial usage. To improve the catalytic activity of UGT76G1, a variant UGT76G1-T284S/M88L/L200A was obtained by structure-guided evolution, whose catalytic activity toward Reb D increased by 2.38 times compared with UGT76G1-T284S. This allowed us to prepare Reb M on a large-scale with a great yield of 90.50%. Moreover, molecular dynamics simulation illustrated that UGT76G1-T284S/M88L/L200A reduced distances from Reb D to catalytic residues and UDPG. Hence, we report an efficient method for the potential scale production of Reb M in this study.
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http://dx.doi.org/10.1021/acs.jafc.2c01209DOI Listing
April 2022

Synovial tissue-derived extracellular vesicles induce chondrocyte inflammation and degradation via NF-κB signalling pathway: An in vitro study.

J Cell Mol Med 2022 04 18;26(7):2038-2048. Epub 2022 Feb 18.

Department of Orthopaedic Surgery, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, China.

Osteoarthritis (OA) is a whole-joint disease characterized by synovial inflammation and cartilage degeneration. However, the relationship between synovial inflammation and cartilage degeneration remains unclear. The modified Hulth's method was adopted to establish a knee OA (KOA) rabbit model. Synovial tissue was collected after 8 weeks, and synovial tissue-derived extracellular vesicles (ST-EVs) were extracted by filtration combined with size exclusion chromatography (SECF), followed by identification through transmission electron microscopy (TEM), nanoparticle tracer analysis (NTA) and Western blot (WB). The collagenase digestion method was used to extract normal rabbit chondrocytes, which were then treated with the SF-EVs to observe the effect and mechanism of SF-EVs on chondrocytes. The morphology, particle size and labelled protein marker detection confirmed that SECF successfully extract ST-EVs. The ST-EVs in the KOA state significantly inhibited chondrocyte proliferation and promoted chondrocytes apoptosis. Moreover, the ST-EVs also promoted the expression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α and COX-2) and cartilage degradation-related enzymes (MMP13, MMP9 and ADAMTS5) in the chondrocytes. Mechanistically, the ST-EVs significantly promoted the activation of NF-κB signalling pathway in chondrocytes. Inhibition the activation of the NF-κB signalling pathway significantly rescued the expression of inflammatory cytokines and cartilage degradation-related enzymes in the ST-EVs-induced chondrocytes. In conclusion, the ST-EVs promote chondrocytes inflammation and degradation by activating the NF-κB signalling pathway, providing novel insights into the occurrence and development of OA.
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http://dx.doi.org/10.1111/jcmm.17227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8980928PMC
April 2022

Cinnamic Aldehyde, the main monomer component of Cinnamon, exhibits anti-inflammatory property in OA synovial fibroblasts via TLR4/MyD88 pathway.

J Cell Mol Med 2022 02 28;26(3):913-924. Epub 2021 Dec 28.

Department of Orthopaedic Surgery, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, China.

Cinnamon is a wildly used traditional Chinese herbal medicine for osteoarthritis (OA) treatment, but the underlying mechanism remains ambiguous. The purpose of this study is to explore the mechanism of cinnamic aldehyde (CA), a bioactive substance extracted from Cinnamon, on synovial inflammation in OA. A total of 144 CA-OA co-targeted genes were identified by detect databases (PubChem, HIT, TCMSP, TTD, DrugBank and GeneCards). The results of GO enrichment analysis indicated that these co-targeted genes have participated in many biological processes including 'inflammatory response', 'cellular response to lipopolysaccharide', 'response to drug', 'immune response', 'lipopolysaccharide-mediated signalling pathway', etc. KEGG pathway analysis showed these co-targeted genes were mainly enriched in 'Toll-like receptor signalling pathway', 'TNF signalling pathway', 'NF-kappa B signalling pathway', etc. Molecular docking demonstrated that CA could successfully bind to TLR2 and TLR4. The results of in vitro experiments showed no potential toxicity of 10, 20 and 50 μM/L CA on human OA FLS, and CA can significantly inhibit the inflammation in LPS-induced human FLS. Further experimental mechanism evidence confirmed CA can inhibited the inflammation in LPS-induced human OA FLS via blocking the TLR4/MyD88 signalling pathway. Our results demonstrated that CA exhibited strong anti-inflammation effect in OA FLS through blocking the activation of TLR4/MyD88 signalling pathway, suggesting its potential as a hopeful candidate for the development of novel agents for the treatment of OA.
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http://dx.doi.org/10.1111/jcmm.17148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8817122PMC
February 2022

Effect of Tai Chi exercise on lower limb function and balance ability in patients with knee osteoarthritis: A protocol of a randomized controlled trial.

Medicine (Baltimore) 2021 Nov;100(46):e27647

Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, China.

Background: Knee osteoarthritis (KOA) is a chronic degenerative joint disease commonly occurring in middle-aged and elderly people. The main clinical manifestations are joint pain, limited activity, and decreased muscle strength resulting in decreased motor control ability. Exercise therapy is an effective method to enhance muscle strength of lower limbs, while China's traditional skill Tai Chi (TC) is a combination of activity and inertia, internal and external exercise therapy. In recent years, scholars at home and abroad have found that regular TC can effectively improve patients' lower limb function and balance ability. The purpose of this study is to explore the effects of TC on lower limb function and balance ability in patients with KOA.

Methods: This is a prospective randomized controlled clinical trial. One hundred forty-six cases of KOA patients will be randomly divided into experimental group and control group according to 1:1 ratio, 73 cases in each group, the control group: sodium hyaluronate; experimental group: TC added on the basis of the control group. Both groups will receive standard treatment for 5 weeks and will be followed up for 3 months. Observation indicators include: the western Ontario and McMaster universities osteoarthritis index; hospital for special surgery knee score; balance stability index, liver and kidney function, adverse reaction rate, etc. SPSS 23.0 software will be used for data analysis.

Discussion: This study will evaluate the effects of TC on lower limb function and balance ability of patients with KOA. The results of this trial will provide a clinical basis for the selection of exercise therapy for patients with KOA.
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http://dx.doi.org/10.1097/MD.0000000000027647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601362PMC
November 2021

Corrosion behavior of high nitrogen nickel-free austenitic stainless steel in the presence of artificial saliva and Streptococcus mutans.

Bioelectrochemistry 2021 Dec 26;142:107940. Epub 2021 Aug 26.

Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang 110819, China.

High nitrogen nickel-free austenitic stainless steels (HNSs) have great potentials to be used in dentistry owing to its exceptional mechanical properties, high corrosion resistance, and biocompatibility. In this study, HNSs with nitrogen of 0.88 wt% and 1.08 wt% displayed much lower maximum pit depths than 316L stainless steel (SS) after 21 d of immersion in abiotic artificial saliva (2.2 μm and 1.7 μm vs. 4.5 μm). Microbiologically influenced corrosion (MIC) evaluations revealed that Streptococcus mutans biofilms led to much severer corrosion of 316L SS than HNSs. Corrosion current densities of HNSs were two orders of magnitude lower than that of 316L SS after incubation of 7 d (37.5 nA/cm and 29.9 nA/cm vs. 5.63 μA/cm). The pitting potentials of HNSs were at least 550 mV higher than that of 316L SS in the presence of S. mutans, confirming the better MIC resistance of HNSs. Cytotoxicity assay confirmed that HNSs were not toxic to MC3T3-E1 cells and allowed better sessile cell growth on them than on 316L SS. It can be concluded that HNSs are more suitable dental materials than the conventional 316L SS.
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http://dx.doi.org/10.1016/j.bioelechem.2021.107940DOI Listing
December 2021

Efficacy and Mechanism of Electroacupuncture Treatment of Rabbits With Different Degrees of Knee Osteoarthritis: A Study Based on Synovial Innate Immune Response.

Front Physiol 2021 5;12:642178. Epub 2021 Aug 5.

Acupuncture and Moxibustion Department, The Third Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, China.

Knee osteoarthritis (KOA) is a chronic degenerative bone and joint disease, which is often clinically manifested as pain, joint swelling, and deformity. Its pathological manifestations are mainly synovial inflammation and cartilage degeneration. This study aims to investigate the efficacy of electro-acupuncture (EA) on model rabbits with varying degrees of KOA and to study the mechanism of EA on KOA based on the innate immune response. Mild and moderate rabbit KOA models were established using a modified Hluth method, and EA was given to both the mild and moderate model groups. The Lequesne-MG index was used to evaluate the behavioral changes in the rabbits before and after EA treatment. Morphological changes in the synovial membrane and cartilage of each group were observed by H&E staining. The Mankin scoring standard and the Krenn scoring standard were used to score the pathology of the cartilage tissue and synovial tissue, respectively. The inflammatory factors and metalloproteinases were detected in the serum of each group by ELISA. The protein and messenger RNA (mRNA) expressions of important elements related to Toll-like receptors (TLRs)-mediated innate immune response in the synovial tissue were detected by Western blot and quantitative PCR (qPCR). The Lequesne-MG index score of the rabbits gradually increased with the modeling prolonged but decreased significantly after EA treatment, indicating that EA has a better effect on alleviating the pain and improving the dysfunction. The morphological analysis showed that the inflammation of and the damage to the synovial membrane and the cartilage tissue gradually deteriorated with the modeling prolonged. However, the synovial membrane inflammation was significantly relieved after EA treatment, and the cartilage injury showed signs of repair. The ELISA analysis showed that, with the modeling prolonged, the serum-related inflammatory factors and mechanism of metalloproteinases gradually increased but decreased after EA treatment. The tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and matrix metalloproteinase3 (MMP3) of EA1 group were significantly lower than those of EA2 group. Both Western blot and qPCR results showed that the protein and mRNA expressions of the elements related to the innate immune response in the synovial membrane increased gradually with the modeling prolonged, but decreased significantly after EA treatment. Additionally, the expression of some components in EA1 group was significantly lower than that in EA2 group. These results confirm that synovial inflammation gradually aggravated with time from the early to mid-stage of KOA. EA alleviated the inflammation and histological changes in KOA rabbits by inhibiting the TLRs-mediated innate synovial immune response. This suggests that using EA in the early stage of KOA may achieve a desirable efficacy.
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http://dx.doi.org/10.3389/fphys.2021.642178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375319PMC
August 2021

Role of Dynamic Navigation Systems in Enhancing the Accuracy of Implant Placement: A Systematic Review and Meta-Analysis of Clinical Studies.

J Oral Maxillofac Surg 2021 10 10;79(10):2061-2070. Epub 2021 Jun 10.

Chief Physician, Department of Oral Implantology, Tianjin Stomatological Hospital, School of Medicine, Nankai University & Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin, China. Electronic address:

Purpose: This systematic review and meta-analysis aims to investigate the influence of dynamic navigation systems on accuracy (platform, apical and angular deviations) in clinical studies.

Methods: The research question was "Do dynamic navigation systems enhance the accuracy of implant placement?" The PubMed, Scopus and Embase databases were used to search the relevant studies up to January 2021. The role of dynamic navigation systems in enhancing the accuracy (platform, apical and angular deviations) of implant placement was then analyzed to conduct a systematic review and meta-analysis.

Results: Eight articles were analyzed in the systematic review and meta-analysis. The systematic review showed that the deviations in implant placement were significantly lower for dynamic navigation than for the freehand method, and there were no significant differences between the dynamic navigation and static guide methods. This meta-analysis showed that the dynamic navigation group exhibited less platform deviation, apical deviation and angular deviation than the control group. The results of subgroup analyses showed that the dynamic navigation group exhibited fewer deviations than the freehand group, and no significant differences were found between the dynamic navigation and static guide groups.

Conclusions: Dynamic navigation resulted in higher accuracy than the freehand method, and similar accuracies were found between dynamic navigation and static guidance for platform deviation, apical deviation or angular deviations.
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http://dx.doi.org/10.1016/j.joms.2021.06.005DOI Listing
October 2021

Can feeding sound attract flower fish (Ptychobarbus kaznakovi)?

J Comp Physiol A Neuroethol Sens Neural Behav Physiol 2021 09 24;207(5):617-627. Epub 2021 Jun 24.

Hubei International Scientific and Technological Cooperation Center of Ecological Protection and Management, Three Gorges Area, China Three Gorges University, Yichang, Hubei Province, 443002, People's Republic of China.

The use of acoustic attractants may have the potential to guide native migratory species towards safe passage. Flower fish Ptychobarbus kaznakovi, a short-distance migratory fish whose population is in decline in the past decades, was exposed to three acoustic stimuli (feeding sound, ambient riverine noise and the pure tone 1000 Hz) to examine the phonotaxic responses using playbacks approaches in a fibreglass tank. The results showed that the flower fish showed significantly greater positive phonotaxis and swam towards the sound sources significantly faster in response to the feeding sounds than to ambient riverine noise and the pure tone during the 5-min exposure. Distribution experiments were conducted to study the preference of flower fish to the three sounds stimuli. The results showed that the experimental fish in feeding sound trials spent significant more time in areas closer to the sound sources than that in the pure tone and the ambient riverine noise trials, respectively. This study indicates that the feeding sounds may serve as potential acoustic attractants to guide flower fish to safe passage routes.
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http://dx.doi.org/10.1007/s00359-021-01501-6DOI Listing
September 2021

Synthesis of 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one from maltol and its taste identification.

Food Chem 2021 Nov 13;361:130052. Epub 2021 May 13.

Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450016, PR China. Electronic address:

2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) exists in many foods, and its effect on taste is controversial. The aim of this study was to clarify whether DDMP has bitter taste or not. For this purpose, DDMP was synthesized from maltol instead of from glucose for the first time. In contrast, DDMP derived from glucose was also prepared and further purified. Their structures were identified by NMR and MS, and considered to be the same substance. The sensory analysis showed that DDMP derived from maltol was tasteless. Further studies indicated that some impurities in Maillard reaction made DDMP derived from glucose taste bitter.
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http://dx.doi.org/10.1016/j.foodchem.2021.130052DOI Listing
November 2021

Synergistic Effect by Polyethylene Glycol as Interfacial Modifier in Silane-Modified Silica-Reinforced Composites.

Polymers (Basel) 2021 Mar 4;13(5). Epub 2021 Mar 4.

Key Laboratory of Rubber-Plastics, Ministry of Education/Shandong Provincial Key Laboratory of Rubber-Plastics, School of Polymer Science and Engineering, Qingdao University of Science & Technology, Qingdao 266042, China.

The viscoelastic behavior and reinforcement mechanism of polyethylene glycol (PEG) as an interfacial modifier in green tire tread composites were investigated in this study. The results show a clear positive effect on overall performance, and it significantly improved all the parameters of the "magic triangle" properties, the abrasion resistance, wet grip and ice traction, as well as the tire rolling resistance, simultaneously. For the preparation of the compounds, two mixing steps were used, as PEG 4000 was added on the second stage in order to avoid the competing reaction between silica/PEG and silanization. Fourier transform infrared spectroscopy (FTIR) confirmed that PEG could cover the silanol groups on the silica surface, resulting in the shortening of cure times and facilitating an increase of productivity. At low content of PEG, the strength was enhanced by the improvement of silica dispersion and the slippage of PEG chains, which are chemically and physically adsorbed on silica surface, but the use of excess PEG uncombined with silica in the compound, i.e., 5 phr, increases the possibility to shield the disulfide bonds of bis(3-(triethoxysilyl)-propyl) tetrasulfide (TESPT), and, thus, the properties were deteriorated. A constrained polymer model was proposed to explain the constrained chains of PEG in the silica-loaded composites on the basis of these results. An optimum PEG content is necessary for moderately strong matrix-filler interaction and, hence, for the enhancement in the mechanical properties.
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http://dx.doi.org/10.3390/polym13050788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961832PMC
March 2021

Cinnamic Aldehyde Inhibits Lipopolysaccharide-Induced Chondrocyte Inflammation and Reduces Cartilage Degeneration by Blocking the Nuclear Factor-Kappa B Signaling Pathway.

Front Pharmacol 2020 5;11:949. Epub 2020 Aug 5.

Department of Orthopedics, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, China.

Osteoarthritis (OA), as one of the top 10 causes of physical disability, is characterized by inflammation of the synovial membrane and progressive destruction of the articular cartilage. Cinnamic aldehyde (CA), an α,β-unsaturated aldehyde extracted from the traditional Chinese herbal medicine cinnamon ( J.Presl), has been reported to have anti-inflammatory, antioxidant, and anticancer properties. However, the anti-inflammatory effect of CA on OA remains unclear. The purpose of the present study was to investigate the effects of CA on inflammation, and cartilage degeneration in OA. A CCK-8 assay was performed to assess the potential toxicity of CA on cultured human OA chondrocytes. Following treatment with lipopolysaccharide (LPS) and CA, the expression of proinflammatory cytokines, including interleukin (IL)-1β, IL-6, and tumor necrosis factor-alfa (TNF-α), was evaluated using quantitative real-time polymerase chain reaction (RT-qPCR) analysis, enzyme-linked immunosorbent assay, and Western blotting (WB). The production of matrix metalloproteinase-13 (MMP-13) and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS-5) was also examined using RT-qPCR and WB. Furthermore, to investigate the potential anti-inflammatory mechanism of CA, biomarkers of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway (p65, IKB-α) were detected using WB. The results demonstrated that CA significantly inhibited the expressions of IL-1β, IL-6, TNF-α, MMP-13, and ADAMTS-5 in LPS-induced OA chondrocytes. CA dramatically suppressed LPS-stimulated NF-κB activation. Collectively, these results suggest that CA treatment may effectively prevent OA.
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http://dx.doi.org/10.3389/fphar.2020.00949DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7419651PMC
August 2020

Mobile Bearing versus Fixed Bearing for Total Knee Arthroplasty: Meta-analysis of Randomized Controlled Trials at Minimum 10-Year Follow-up.

J Knee Surg 2022 Jan 26;35(2):135-144. Epub 2020 Jun 26.

Department of Orthopaedic Surgery, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, People's Republic of China.

This meta-analysis aimed to compare the clinical and radiographic outcomes between mobile-bearing total knee arthroplasty (MB-TKA) and fixed-bearing total knee arthroplasty (FB-TKA) at a minimum 10-year follow-up. PubMed, EMBASE, and Cochrane databases were searched. All included articles were evaluated by two trained reviewers according to the guidelines of the Cochrane Collaboration Handbook for potential risk, and the Consolidated Standards on Reporting Trials (CONSORT) checklist and scoring system was also used to assess the methodological quality of each study. The extracted data included function scores, range of motion (ROM) of the knee, incidence of adverse events or revision, survivorship analysis, and radiographic outcomes. Seven randomized controlled trials (RCTs) were included in this meta-analysis, and all RCTs had a follow-up period longer than 10 years. This meta-analysis shows no significant difference between the two groups with respect to the Keen Society Score (KSS;  = 0.38), KSS function score ( = 0.30), the Western Ontario and McMaster Universities Osteoarthritis index (WOMAC;  = 0.59), ROM ( = 0.71), radiolucent line ( = 0.45), femoral and tibial component positions in the coronal plane ( = 0.55 and 0.35, respectively), revision incidence ( = 0.77), and survivorship rates ( = 0.39). Meanwhile, it showed a slight difference between the two groups in the tibial component position in the sagittal plane ( = 0.003). According to this meta-analysis, the current best available evidence suggests no significant difference between the MB-TKA and FB-TKA groups with respect to the clinical outcomes, radiographic outcomes, revision, and survivorship at a minimum 10-year follow-up. This is a Level II, meta-analysis study.
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http://dx.doi.org/10.1055/s-0040-1713356DOI Listing
January 2022

Genome-Wide Characterization and Comparative Analysis of MYB Transcription Factors in Species.

G3 (Bethesda) 2020 08 5;10(8):2653-2660. Epub 2020 Aug 5.

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

Numerous studies in plants have shown the vital roles of MYB transcription factors in signal transduction, developmental regulation, biotic/abiotic stress responses and secondary metabolism regulation. However, less is known about the functions of in In this study, five medicinal macrofungi of genus were subjected to a genome-wide comparative analysis of genes. A total of 75 genes were identified and classified into four types: 1R-MYBs (52), 2R-MYBs (19), 3R-MYBs (2) and 4R-MYBs (2). Gene structure analysis revealed varying exon numbers (3-14) and intron lengths (7-1058 bp), and noncanonical GC-AG introns were detected in and In a phylogenetic analysis, 69 out of 75 genes were clustered into 15 subgroups, and both single-copy orthologous genes and duplicated genes were identified. The promoters of the genes harbored multiple -elements, and specific genes were co-expressed with the genes, indicating the potential roles of these genes in stress response, development and metabolism. This comprehensive and systematic study of MYB family members provides a reference and solid foundation for further functional analysis of genes in species.
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http://dx.doi.org/10.1534/g3.120.401372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407476PMC
August 2020

Extraction and identification of synovial tissue-derived exosomes by different separation techniques.

J Orthop Surg Res 2020 Mar 9;15(1):97. Epub 2020 Mar 9.

Department of Orthopaedic Surgery, Beijing University of Chinese Medicine Third Affiliated Hospital, No. 51, Xiaoguan Street, Anding Gate, Chaoyang District, Beijing, China.

Objective: The aim of this study is to compare the efficiency of different separation techniques for extracting synovial tissue-derived exosomes.

Methods: The synovial tissue discarded during knee arthroscopy or total knee arthroplasty surgery was collected from the Third Affiliated Hospital of Beijing University of Chinese Medicine. Ultracentrifugation (UC), filtration combined with size exclusion chromatography (SECF), and 8% polyethylene glycol (PEG) were used to extract synovial tissue-derived exosomes. Transmission electron microscopy (TEM), nanoparticle tracer analysis (NTA), and Western Blot (WB) were used to detect the morphology, particle size, and biomarker proteins (CD9, CD63, Flotillin-1, and calnexin) of exosomes.

Results: The extracts of enriched round and discoid vesicles were successfully extracted with UC, SECF, and PEG. The results of TEM have shown that all three extraction methods can extract circular or elliptical vesicles with disc- and cup-shaped structures from the synovial tissue, with the diameter is about 30-150 nm. NTA suggested the main peaks of three groups of exosomes are around 100-120 nm, and the concentration of the three groups of exosomes was greater than 1 × 10/ml. The results of WB showed that three positive protein markers (CD9, CD63, and Flotillin-1) were highly expressed in the suspension extracted by the three methods and low in the synovial tissue. However, the negative protein (calnexin) was highly expressed in synovial tissues and PEG group, while low in UC and SECF group.

Conclusion: Morphology, particle size, and labeled protein marker detection confirmed that UC, SECF, and PEG can extract exosomes derived from synovial tissue; UC and SECF are more recommended for the extraction of synovial tissue-derived exosomes, which provides a methodological basis for studying the function and mechanism of synovial tissue exosomes in the future.
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http://dx.doi.org/10.1186/s13018-020-01604-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063768PMC
March 2020

Intra-articular platelet-rich plasma injection for knee osteoarthritis: a summary of meta-analyses.

J Orthop Surg Res 2019 Nov 27;14(1):385. Epub 2019 Nov 27.

Department of Orthopaedic Surgery, Beijing University of Chinese Medicine Third Affiliated Hospital, No. 51, XiaoGuan street, AnDing gate, ChaoYang district, Beijing, China.

Objective: The purpose of this study was (1) to perform a summary of meta-analyses comparing platelet-rich plasma (PRP) injection with hyaluronic acid (HA) and placebo injection for KOA patients, (2) to determine which meta-analysis provides the best available evidence to making proposals for the use of PRP in the treatment of KOA patients, and (3) to highlight gaps in the literature that require future investigation.

Material And Methods: PubMed, EMBASE, and Cochrane databases search were performed for meta-analyses which compared PRP injection with HA or placebo. Clinical outcomes and adverse events were extracted from these meta-analyses. Meta-analysis quality was assessed using the Quality of Reporting of Meta-analyses (QUOROM) systems and the Oxman-Guyatt quality appraisal tool. The Jadad decision algorithm was also used to determine which meta-analysis provided the best available evidence.

Results: Four meta-analyses were included in our study, and all of these articles were Level I evidence. The QUOROM score of each included meta-analysis range from 14 to 17 points (mean score 15, maximum score 18), and the Oxman-Guyatt score range from 4 to 6 points (mean score 5, maximum score 7). Three meta-analyses indicated PRP showed more benefit in pain relief and functional improvement than the control group, and the other one suggested no difference between these groups. All included meta-analyses found no statistical difference in adverse events between these groups. In addition, a meta-analysis conducted by Shen et al. got the highest methodological quality score and suggested that PRP provided better pain relief and function improvement in the treatment of KOA.

Conclusions: For short-term follow-up (≤1 year), intra-articular PRP injection is more effective in terms of pain relief and function improvement in the treatment of KOA patients than HA and placebo, and there is no difference in the risk of an adverse event between PRP and HA or placebo.

Level Of Evidence: Level I evidence, a summary of meta-analyses TRIAL REGISTRATION: PROSPERO ID CRD42018116168.
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http://dx.doi.org/10.1186/s13018-019-1363-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880602PMC
November 2019

Remote Light-Responsive Nanocarriers for Controlled Drug Delivery: Advances and Perspectives.

Small 2019 11 10;15(45):e1903060. Epub 2019 Oct 10.

Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, QLD, 4072, Brisbane, Australia.

Engineering of smart photoactivated nanomaterials for targeted drug delivery systems (DDS) has recently attracted considerable research interest as light enables precise and accurate controlled release of drug molecules in specific diseased cells and/or tissues in a highly spatial and temporal manner. In general, the development of appropriate light-triggered DDS relies on processes of photolysis, photoisomerization, photo-cross-linking/un-cross-linking, and photoreduction, which are normally sensitive to ultraviolet (UV) or visible (Vis) light irradiation. Considering the issues of poor tissue penetration and high phototoxicity of these high-energy photons of UV/Vis light, recently nanocarriers have been developed based on light-response to low-energy photon irradiation, in particular for the light wavelengths located in the near infrared (NIR) range. NIR light-triggered drug release systems are normally achieved by using two-photon absorption and photon upconversion processes. Herein, recent advances of light-responsive nanoplatforms for controlled drug release are reviewed, covering the mechanism of light responsive small molecules and polymers, UV and Vis light responsive nanocarriers, and NIR light responsive nanocarriers. NIR-light triggered drug delivery by two-photon excitation and upconversion luminescence strategies is also included. In addition, the challenges and future perspectives for the development of light triggered DDS are highlighted.
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http://dx.doi.org/10.1002/smll.201903060DOI Listing
November 2019

Changes in blood lactate and muscle activation in elite rock climbers during a 15-m speed climb.

Eur J Appl Physiol 2019 Mar 28;119(3):791-800. Epub 2019 Jan 28.

Department of Human Performance and Health Education, College of Human Development and Education, Western Michigan University, Kalamazoo, USA.

Purpose: The purpose of this study was to investigate the changes in blood lactate concentration (BL) and muscle activity patterns during a 15-m speed climbing competition that consisted of ten consecutive climbing actions on a standardized artificial wall in trained rock climbers.

Methods: Twelve trained rock climbers participated in this study. Surface electromyography (sEMG) and video signals were synchronized and recorded during climbing. The blood lactate was also tested 3 min after completing the climb.

Results: The average climbing time was 8.1 ± 2.1 s for the 15-m speed climb across all subjects, accompanied by a BL of 7.6 ± 1.9 mmol/L. The climbing speed and power firstly increased and then slightly decreased relative to peak value during the 15-m speed climbing. The results showed there was a positive correlation between the BL and the climbing time, r = 0.59, P = 0.043. The sEMG showed the flexor digitorum superficialis (FDS) electric activity was the highest, followed by the biceps brachii (BB) and latissimus dorsi. The instantaneous median frequency of sEMG of FDS and BB significantly decreased during the 15-m speed climbing. All the participants showed the higher sEMG RMS (%) in the terminal phase than that in the initial phase, especially with a greater increase in the left upper limbs. However, the lower limbs muscles presented no significant changes in the sEMG amplitude during climbing.

Conclusions: The FDS and BB play an important role in completing the 15-m speed climbing. The median frequency of arm EMG decreased more than that of legs, suggesting more fatigue. The blood lactate concentration increases in the current study suggest that a certain amount of glycolysis supplies energy in completing 15-m speed rock climbing. Based on the current data, it is suggested that muscular endurance of FDS and BB muscles in upper limbs should be improved for our climbers in this study.
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http://dx.doi.org/10.1007/s00421-018-04070-wDOI Listing
March 2019

Comparison of the Mitochondrial Genome Sequences of Six Isolates Suggests Short Fragment Insertions as a Potential Factor Leading to Larger Genomic Size.

Front Microbiol 2018 10;9:2079. Epub 2018 Sep 10.

Center for Genomics and Biotechnology, Haixia Institute of Science and Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

Mitochondrial DNA (mtDNA) is a core non-nuclear genetic material found in all eukaryotic organisms, the size of which varies extensively in the eumycota, even within species. In this study, mitochondrial genomes of six isolates of (Lév.) were assembled from raw reads from PacBio and Illumina sequencing. The diversity of genomic structures, conserved genes, intergenic regions and introns were analyzed and compared. Genome sizes ranged from 132 to 147 kb and contained the same sets of conserved protein-coding, tRNA and rRNA genes and shared the same gene arrangements and orientation. In addition, most intergenic regions were homogeneous and had similar sizes except for the region between cytochrome b (cob) and cytochrome c oxidase I (cox1) genes which ranged from 2,998 to 8,039 bp among the six isolates. Sixty-five intron insertion sites and 99 different introns were detected in these genomes. Each genome contained 45 or more introns, which varied in distribution and content. Introns from homologous insertion sites also showed high diversity in size, type and content. Comparison of introns at the same loci showed some complex introns, such as twintrons and ORF-less introns. There were 44 short fragment insertions detected within introns, intergenic regions, or as introns, some of them located at conserved domain regions of homing endonuclease genes. Insertions of short fragments such as small inverted repeats might affect or hinder the movement of introns, and these allowed for intron accumulation in the mitochondrial genomes analyzed, and enlarged their size. This study showed that the evolution of fungal mitochondrial introns is complex, and the results suggest short fragment insertions as a potential factor leading to larger mitochondrial genomes in .
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http://dx.doi.org/10.3389/fmicb.2018.02079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6140425PMC
September 2018

Histomorphology and innate immunity during the progression of osteoarthritis: Does synovitis affect cartilage degradation?

J Cell Physiol 2018 Feb 15;233(2):1342-1358. Epub 2017 Jun 15.

The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing, China.

Osteoarthritis (OA) is a common chronic degenerative disease that affects all joints. At present, the pathological processes and mechanisms of OA are still unclear. Innate immunity, a key player in damage to the structure of the joint and the mechanism by which the host attempts to repair OA, affects all pathological stages of the disease. In the present study, our aim was to assess changes in innate immunity during the pathological processes of OA in articular cartilage (AC) and the synovial membrane (SM), which are the major structures in joints, and to systematically examine the histological changes in AC and SM in mild, moderate and severe cases of OA, in order to further speculate about the manner in which the interactions of AC and SM are facilitated by innate immunity. Histological methods (including HE and Safranin O-fast green staining), immunofluorescent double staining, TUNEL stain, and Western blots were used to assess the morphological changes within AC and SM tissues in healthy and mild, moderate, or severe OA rats. Our results showed that the damage to AC and SM within the joints progressively worsened in different degrees during the course of the disease, and that the innate immune system was closely involved in the AC and SM during each stage of OA. These findings also confirmed that SM may affect the pathological changes in AC through the innate immune system, and therefore affect the progress of OA.
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http://dx.doi.org/10.1002/jcp.26011DOI Listing
February 2018

In Situ Formation of Polysulfonamide Supported Poly(ethylene glycol) Divinyl Ether Based Polymer Electrolyte toward Monolithic Sodium Ion Batteries.

Small 2017 Jan 3;13(2). Epub 2016 Nov 3.

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

Sodium ion battery is one of the promising rechargeable batteries due to the low-cost and abundant sodium sources. In this work, a monolithic sodium ion battery based on a Na V (PO ) cathode, MoS layered anode, and polyether-based polymer electrolyte is reported. In addition, a new kind of polysulfonamide-supported poly(ethylene glycol) divinyl ether based polymer electrolyte is also demonstrated for monolithic sodium ion battery via in situ preparation. The resultant polymer electrolyte exhibits relatively high ionic conductivity (1.2 mS cm ) at ambient temperature, wide electrochemical window (4.7 V), and favorable mechanical strength (25 MPa). Moreover, such a monolithic Na V (PO ) /MoS sodium ion battery using this polymer electrolyte delivers outstanding rate capability (up to 10 C) and superior cyclic stability (84%) after 1000 cycles at 0.5 C. What is more essential, such a polymer electrolyte based soft-package monolithic sodium ion cell can still power a red light emitting diode lamp and run finite times without suffering from any internal short-circuit failures, even in the case of a bended and wrinkled state. Considering these aspects, this work no doubt provides a new approach for the design of a high-performance polymer electrolyte toward monolithic sodium ion battery with exceptional rate capability and high safety.
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http://dx.doi.org/10.1002/smll.201601530DOI Listing
January 2017

Diruthenium(ii)-NNN pincer complex catalysts for transfer hydrogenation of ketones.

Dalton Trans 2016 Nov 24;45(44):17843-17849. Epub 2016 Oct 24.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, P. R. China.

Dinuclear ruthenium(ii)-NNN pincer complexes bearing a π linker-supported bis(pyrazolyl-imidazolyl-pyridine) ligand were synthesized and structurally characterized, and they exhibited excellent catalytic activity for the transfer hydrogenation of ketones in refluxing isopropanol, reaching TOF values up to 1.3 × 10 h. Compared with the corresponding mononuclear Ru(ii)-NNN pincer complexes, the bimetallic complexes could be applied at concentrations as low as 0.03 mol% Ru and demonstrated remarkably enhanced catalytic activity in the transfer hydrogenation reactions of ketones. The high catalytic activity of the diruthenium(ii) complexes is attributed to the excellent stability and possible cooperativity of the two coordinated Ru(ii) metal centers through the π linker. The present synthetic methodology has established an applicable strategy to construct highly active bimetallic NNN pincer complex catalysts.
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http://dx.doi.org/10.1039/c6dt03620aDOI Listing
November 2016
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