Publications by authors named "Qingfeng Guo"

26 Publications

  • Page 1 of 1

Temporary Ligation of the Bilateral Uterine Arteries During Laparoscopy Combined with Hysteroscopy in the Treatment of Caesarean Scar Pregnancy: Experience at a Chinese Teaching Hospital.

Int J Gen Med 2021 26;14:2087-2094. Epub 2021 May 26.

Department of Gynecology, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, 361000, People's Republic of China.

Purpose: This study aimed to investigate the clinical efficacy of temporary ligation of the bilateral uterine arteries during laparoscopy combined with hysteroscopy in the treatment of caesarean scar pregnancy (CSP).

Patients And Methods: This study was a retrospective analysis of 83 patients who had initially undergone laparoscopy combined with hysteroscopy for CSP between 2012 and 2018 at Xiamen Women and Children's Hospital. Patients were assigned to the ligation group or the no ligation group according to whether they underwent temporary ligation of the bilateral uterine arteries. Factors extracted from the database included general preoperative information, surgical indicators (intraoperative blood loss, operation time, and blood transfusion), postoperative recovery indicators (β-hCG on day 3 after surgery, time to β-hCG normalization), and postoperative complications (decrease in menstrual bleeding, alteration in the menstrual cycle) and were compared between the two groups.

Results: The intraoperative blood loss of patients in the ligation group was significantly less than that of patients in the no ligation group (P=0.027), especially in patients with higher serum β-hCG level (P=0.030). No significant differences in the operation time, blood transfusion, decline ratio of hCG on day 3 after surgery, reduction in haemoglobin and haematocrit value, decrease in menstrual bleeding, or alteration in the menstrual cycle were observed between the two groups (P>0.05).

Conclusion: For CSP patients, temporary ligation of the bilateral uterine arteries during laparoscopy combined with hysteroscopy achieved better clinical outcomes than laparoscopy combined with hysteroscopy without ligation with respect to intraoperative blood loss. This approach offers effective and safe surgical management for CSP in clinical practice.
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http://dx.doi.org/10.2147/IJGM.S306462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165297PMC
May 2021

Hydrazine-Containing Heterocycle Cytochalasan Derivatives From Hydrazinolysis of Extracts of a Desert Soil-Derived Fungus 375.

Front Chem 2021 21;9:620589. Epub 2021 Apr 21.

Zhengzhou Key Laboratory of Synthetic Biology of Natural Products, Zhengzhou Key Laboratory of Medicinal Resources Research, Comprehensive Utilization of Edible and Medicinal Plant Resources Engineering Technology Research Center, Huanghe Science and Technology College, Zhengzhou, China.

"Diversity-enhanced extracts" is an effective method of producing chemical libraries for the purpose of drug discovery. Three rare new cytochalasan derivative chaetoglobosins B-B (-) were obtained from chemically engineered crude broth extracts of 375 prepared by reacting with hydrazine monohydrate and four known metabolite chaetoglobosins () were also identified from the fungus. The structures were identified by NMR and MS analysis and electronic circular dichroism simulation. In addition, the antiproliferative activities of these compounds were also evaluated, and the drug-resistant activities of cytochalasans were evaluated for the first time. Compound possessed potent activity against four human cancer cells (A549, HCC827, SW620, and MDA-MB-231), and two drug-resistant HCC827 cells (Gefitinib-resistant, Osimertinib-resistant) compared with the positive controls.
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http://dx.doi.org/10.3389/fchem.2021.620589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097171PMC
April 2021

The assessment of circulating cell-free DNA as a diagnostic tool for breast cancer: an updated systematic review and meta-analysis of quantitative and qualitative ssays.

Clin Chem Lab Med 2021 May 5. Epub 2021 May 5.

Department of General Surgery, Affiliated Hospital of Jiangnan University (Original Area of Wuxi No. 3 People's Hospital), Wuxi, P. R. China.

Objectives: This updated meta-analysis aimed to assess the diagnostic accuracy of circulating cell-free DNA (cfDNA) in breast cancer (BC).

Content: An extensive systematic search was performed in PubMed, Scopus, Embase, and Science Direct databases to retrieve all related literature. Various diagnostic estimates, including sensitivity (SE), specificity (SP), likelihood ratios (LRs), diagnostic odds ratio (DOR), and area under the curve (AUC) of summary receiver operating characteristic (sROC) curve, were also calculated using bivariate linear mixed models.

Summary: In this meta-analysis, 57 unique articles (130 assays) on 4246 BC patients and 2,952 controls, were enrolled. For quantitative approaches, pooled SE, SP, PLR, NLR, DOR, and AUC were obtained as 0.80, 0.88, 6.7, 0.23, 29, and 0.91, respectively. Moreover, for qualitative approaches, pooled SE and SP for diagnostic performance were obtained as 0.36 and 0.98, respectively. In addition, PLR was 14.9 and NLR was 0.66. As well, the combined DOR was 23, and the AUC was 0.79.

Outlook: Regardless of promising SE and SP, analysis of LRs suggested that quantitative assays are not robust enough neither for BC confirmation nor for its exclusion. On the other hand, qualitative assays showed satisfying performance only for confirming the diagnosis of BC, but not for its exclusion. Furthermore, qualitative cfDNA assays showed a better diagnostic performance in patients at the advanced stage of cancer, which represented no remarkable clinical significance as a biomarker for early detection.
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http://dx.doi.org/10.1515/cclm-2021-0193DOI Listing
May 2021

WeChat-based education and rehabilitation program in unprotected left main coronary artery disease patients after coronary artery bypass grafting: an effective approach in reducing anxiety, depression, loss to follow-up, and improving quality of life.

Braz J Med Biol Res 2021 12;54(4):e10370. Epub 2021 Feb 12.

Ministry of Nursing, 4th Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

This study aimed to investigate the effect of WeChat-based education and rehabilitation program (WERP) on anxiety, depression, health-related quality of life (HRQoL), major adverse cardiac/cerebrovascular events (MACCE)-free survival, and loss to follow-up rate in unprotected left main coronary artery disease (ULMCAD) patients after coronary artery bypass grafting (CABG). In this randomized controlled study, 140 ULMCAD patients who underwent CABG were randomly assigned to WERP group (n=70) or control care (CC) group (n=70). During the 12-month intervention period, anxiety and depression (using hospital anxiety and depression scale (HADS)) and HRQoL (using 12-Item Short-Form Health Survey (SF-12)) were assessed longitudinally. During the total 36-month follow-up period (12-month intervention and 24-month non-intervention periods), MACCE and loss to follow-up were recorded. During the intervention period, HADS-anxiety score at month 9 (M9) (P=0.047) and month 12 (M12) (P=0.034), anxiety rate at M12 (P=0.028), and HADS-D score at M12 (P=0.048) were all reduced in WERP group compared with CC group. As for HRQoL, SF-12 physical component summary score at M9 (P=0.020) and M12 (P=0.010) and SF-12 mental component summary score at M9 (P=0.040) and M12 (P=0.028) were all increased in WERP group compared with CC group. During the total follow-up period, WERP group displayed a trend of longer MACCE-free survival than that in CC group but without statistical significance (P=0.195). Additionally, loss to follow-up rate was attenuated in WERP group compared with CC group (P=0.033). WERP serves as an effective approach in optimizing mental health care and promoting life quality in ULMCAD patients after CABG.
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http://dx.doi.org/10.1590/1414-431X202010370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894386PMC
February 2021

Anticancer Effects and Mechanisms of Action of Plumbagin: Review of Research Advances.

Biomed Res Int 2020 1;2020:6940953. Epub 2020 Dec 1.

Zhengzhou Key Laboratory of Medicinal Resources Research, Huanghe Science and Technology College, Zhengzhou 450063, China.

Plumbagin (PLB), a natural naphthoquinone constituent isolated from the roots of the medicinal plant L., exhibited anticancer activity against a variety of cancer cell lines including breast cancer, hepatoma, leukemia, melanoma, prostate cancer, brain tumor, tongue squamous cell carcinoma, esophageal cancer, oral squamous cell carcinoma, lung cancer, kidney adenocarcinoma, cholangiocarcinoma, gastric cancer, lymphocyte carcinoma, osteosarcoma, and canine cancer. PLB played anticancer activity many molecular mechanisms, such as targeting apoptosis, autophagy pathway, cell cycle arrest, antiangiogenesis pathway, anti-invasion, and antimetastasis pathway. Among these signaling pathways, the key regulatory genes regulated by PLB were NF-k, STAT3, and AKT. PLB also acted as a potent inducer of reactive oxygen species (ROS), suppressor of cellular glutathione, and novel proteasome inhibitor, causing DNA double-strand break by oxidative DNA base damage. This review comprehensively summarizes the anticancer activity and mechanism of PLB.
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http://dx.doi.org/10.1155/2020/6940953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7725562PMC
December 2020

Chaetoglobosin G inhibits proliferation, autophagy and cell cycle of lung cancer cells through EGFR/MEK/ERK signaling pathway.

Pharmazie 2020 12;75(12):642-645

Chaetoglobosin G (CG) is a fungal secondary metabolite and shows anti-tumor effects. However, the mechanisms behind the anti-tumor effect is still unclear. In this study, we evaluated the anti-proliferation effect of CG on human NSCLC A549 cells and explored the underlying mechanisms. The anti-proliferation effect of CG on A549 cells was evaluated by MTT. The targets of CG were screened through transcriptome sequencing. A flow cytometer was used to detect cell cycle and apoptosis. Western blotting was used to analyze apoptosis, cell cycle and autophagy related protein expression. Our results showed that CG had a dose-dependent inhibitory effect on proliferation of A549 cells. Transcriptome sequencing analysis found that CG obviously induced cell cycle arrest. Flow cytometry analysis and western blot showed that CG induced G2/M arrest with p21 protein upregulation and cyclinB1 protein downregulation. Western blot analysis also indicated that p-EGFR, EGFR, p-MEk and p-ERK protein expressions decreased and autophagy protein LC3II expression increased, indicating that CG can promote autophagy through EGFR/MEK/ERK/LC3 pathway. Moreover, CG can induce apoptosis with bcl-2 protein decrease. In conclusion, this study indicated that CG obviously inhibited A549 cell proliferation, and its mechanism may induce autophagy of A549 cells through EGFR/MEK/ERK/LC3 pathway to upregulate the expression of P21, thus lead to G2/M phase arrest to exert an anti-tumor role.
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http://dx.doi.org/10.1691/ph.2020.0750DOI Listing
December 2020

Anti-inflammatory and antioxidant effects of Chaetoglobosin V in LPS-induced RAW264.7 cells: Achieved via the MAPK and NF-κB signaling pathways.

Food Chem Toxicol 2021 Jan 5;147:111915. Epub 2020 Dec 5.

National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng, Henan Province, 475004, China. Electronic address:

There are few reports on the biological activities of chaetoglobosin V (Cha V) (a cytochalasin alkaloid). In this study, we investigated the molecular mechanisms underlying the anti-inflammatory and antioxidant effects of Cha V in the RAW264.7 cells stimulated lipopolysaccharide (LPS). LPS stimulation-induced oxidative stress (i.e. increase production of reactive oxygen species (ROS) and decreased expression of antioxidant superoxide dismutase (SOD)) was suppressed after a Cha V treatment. Cha V could significantly inhibit the upregulated expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) gene and protein induced by LPS whilst attenuating the production of pro-inflammatory cytokines TNF-α, IL-6 and IL-1β. Such antioxidant and anti-inflammatory effects were achieved through the TLR4-mediated MyD88-dependent signaling pathways (via suppressing the phosphorylation of p38, ERK, JNK MAPK and translocation of the NF-κB p65 subunit into nucleus), and the TRIF-dependent signaling pathways (via reducing IFN-β release without inhibiting interferon-regulated factor 3 (IRF3) and IRF7). At 25-100 μM (a concentration range with no cytotoxicity), Cha V dose-dependently influenced SOD enzyme activity and phosphorylation of p38, ERK1/2 and JNK, and at 100 μM, likely exerted the greatest inhibition towards LPS-induced oxidative stress and inflammatory response via the MAPK and NF-κB signaling pathway.
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http://dx.doi.org/10.1016/j.fct.2020.111915DOI Listing
January 2021

Bioactivities and Future Perspectives of Chaetoglobosins.

Evid Based Complement Alternat Med 2020 24;2020:8574084. Epub 2020 Mar 24.

Department of Pharmacy, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China.

Chaetoglobosins belonging to cytochalasan alkaloids represent a large class of fungal secondary metabolites. To date, around 100 chaetoglobosins and their analogues have been isolated and identified over the years from a variety of fungi, mainly from the fungus . Studies have found that chaetoglobosins possess a broad range of biological activities, including antitumor, antifungal, phytotoxic, fibrinolytic, antibacterial, nematicidal, anti-inflammatory, and anti-HIV activities. This review will comprehensively summarize the biological activities and mechanisms of action of nature-derived chaetoglobosins.
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http://dx.doi.org/10.1155/2020/8574084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7132351PMC
March 2020

Color and genesis of californite from Pakistan: insights from μ-XRF mapping, optical spectra and X-ray photoelectron spectroscopy.

Sci Rep 2020 01 14;10(1):285. Epub 2020 Jan 14.

School of Gemmology, China University of Geosciences, Beijing, 100083, China.

Four californite samples from Pakistan with yellowish-green, green and reddish-brown colors were investigated by combining the methods of μ-XRF mapping, XRD, Raman spectra, optical spectra, EPMA and XPS. The results show that the californite is composed mainly of microcrystalline vesuvianite and smaller amounts of clinochlore. Based on the distribution of the clinochlore, the californite can be divided into three types. The gem-quality californite is composed of microcrystalline vesuvianite and has a translucent appearance. The ordinary-quality californite contains microcrystalline vesuvianite as well as clinochlore, and it has an opaque appearance. The transitional-type has properties that are intermediate between those of gem- and ordinary-quality californite. Octahedrally coordinated iron and chromium in the clinochlore reduce the transparency and contribute to the opaque green and yellowish-green colors of the californite. At sites where there is no clinochlore, Cr in the octahedrally coordinated site Y3 of the vesuvianite is mainly responsible for the green tone of the californite, Fe and Mn at the Y3 site contribute mainly to the yellowish-green and reddish-brown colors, respectively. The Fe → Fe charge transfer also occurs in vesuvianite and partly influences the appearance of the californite. The actual color of californite that lacks clinochlore is due to the synergy of Cr, Fe and Mn crystal field transfers at the octahedral site Y3 as well as the Fe → Fe charge transfer in the vesuvianite. Vesuvianite in the californite can be assigned to the P4/n space group, and the occurrence of clinochlore reflects the fact that the californite from Pakistan formed under medium-grade metamorphic conditions at temperatures of ~300-500 °C. The content of clinochlore provides a basis for grading the quality of the californite.
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http://dx.doi.org/10.1038/s41598-019-57186-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6959299PMC
January 2020

Two Novel Polysaccharides in L and anti-A549 Lung Cancer Cells Activity .

Molecules 2019 Oct 16;24(20). Epub 2019 Oct 16.

Zhengzhou Key Laboratory of Medicinal Resources Research, Huanghe Science and Technology College, Zhengzhou 450063, China.

Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from L. Their average molecular weights were 2.721 × 10 and 2.850 × 10. PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different. Based on methylation and NMR spectroscopy, the part structure of PCp-I was identified. The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved. The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability. Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC = 64.84 and 126.30 μM) .
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http://dx.doi.org/10.3390/molecules24203733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6833038PMC
October 2019

Pharmacological Effects of Verticine: Current Status.

Evid Based Complement Alternat Med 2019 7;2019:2394605. Epub 2019 Mar 7.

Zhengzhou Key Laboratory of Medicinal Resources Research, Huanghe Science and Technology College, Zhengzhou 450063, China.

Verticine is the major bioactive constituent of as a kind of Traditional Chinese Medicine. Pharmacological researches have reported various benefits of verticine, including anticancer, anti-inflammatory, protecting against acute lung injury, tracheobronchial relaxation, antitussive, expectorant, sedative, and analgesic activities, in addition to inhibiting proliferation of cultured orbital fibroblast, angiotensin converting enzyme (ACE), and acetylcholinesterase (AChE) and inhibiting hERG potassium channels. The underlying mechanisms of verticine are still under investigation. This review will comprehensively summarize the metabolism, biological activities, and possible mechanism of verticine.
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http://dx.doi.org/10.1155/2019/2394605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431433PMC
March 2019

Optical Pressure Sensor Based on the Emission and Excitation Band Width (fwhm) and Luminescence Shift of Ce-Doped Fluorapatite-High-Pressure Sensing.

ACS Appl Mater Interfaces 2019 Jan 17;11(4):4131-4138. Epub 2019 Jan 17.

Adam Mickiewicz University , Faculty of Chemistry, Department of Rare Earths , Umultowska 89b , 61-614 Poznań , Poland.

A novel, contactless optical sensor of pressure based on the luminescence red-shift and bandwidth (full width at half-maximum, fwhm) of the Ce-doped fluorapatite-YBa(SiO)F powder has been successfully synthesized via a facile solid-state method. The obtained material exhibits a bright blue emission under UV light excitation. It was characterized using powder X-ray diffraction, scanning electron microscopy and luminescence spectroscopy, including high-pressure measurements of excitation and emission spectra, up to above ∼30 GPa. Compression of the material resulted in a significant red-shift of the allowed 4f → 5d and 5d → 4f transitions of Ce in the excitation and emission spectra, respectively. The pressure-induced monotonic shift of the emission band, as well as changes in the excitation/emission band widths, have been correlated with pressure for sensing purposes. The material exhibits a high pressure sensitivity (dλ/d P ≈ 0.63 nm/GPa) and outstanding signal intensity at high-pressure conditions (∼90% of the initial intensity at around 20 GPa) with minimal pressure-induced quenching of luminescence.
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http://dx.doi.org/10.1021/acsami.8b19500DOI Listing
January 2019

Metabolomic comparison between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris.

Biomed Chromatogr 2018 May 11:e4279. Epub 2018 May 11.

Zhengzhou Key Laboratory of Medicinal Resources Research, Institute of Nanostructured Functional Materials, Huanghe Science and Technology College, Zhengzhou, Henan, China.

A systematic study on the metabolome differences between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris was conducted using liquid chromatography-mass spectrometry. Principal component analysis and orthogonal projection on latent structure-discriminant analysis results showed that C. militaris grown on solid rice medium (R-CM) and C. militaris grown on tussah pupa (T-CM) evidently separated and individually separated from wild O. sinensis, indicating metabolome difference among wild O. sinensis, R-CM and T-CM. The metabolome differences between R-CM and T-CM indicated that C. militaris could accommodate to culture medium by differential metabolic regulation. Hierarchical clustering analysis was further performed to cluster the differential metabolites and samples based on their metabolic similarity. The higher content of amino acids (pyroglutamic acid, glutamic acid, histidine, phenylalanine and arginine), unsaturated fatty acid (linolenic acid and linoleic acid), peptides, mannitol, adenosine and succinoadenosine in O. sinensis make it as an excellent choice as a traditional Chinese medicine for invigoration or nutritional supplementation. Similar compositions with O. sinensis and easy cultivation make artificially cultured C. militaris a possible alternative to O. sinensis.
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http://dx.doi.org/10.1002/bmc.4279DOI Listing
May 2018

New insight into PM pollution patterns in Beijing based on one-year measurement of chemical compositions.

Sci Total Environ 2018 Apr 1;621:734-743. Epub 2017 Dec 1.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, China.

In recent years, air pollution has become a major concern in China, especially in the capital city of Beijing. Haze events occur in Beijing over all four seasons, exhibiting distinct characteristics. In this study, the typical evolution patterns of atmospheric particulate matter with a diameter of less than 2.5μm (PM) in each season were illustrated by episode-based analysis. In addition, a novel method was developed to elucidate the driving species of pollution, which is the largest contributor to the incremental PM (ΔPM), not PM. This method revealed a temporal variation of the driving species throughout the year: nitrate-driven spring, sulfate-driven summer, nitrate-driven early fall, and organic matters (OM)-driven late fall and winter. These results suggested that primary organic particles or volatile organic compounds emissions were dominant in the heating season due to residential heating, while NOx and SO emissions dominated in the other seasons. Besides, nitrate formation seemed more significant than sulfate formation during severe pollution episodes. It was also found that the pollution formation mechanism in the winter showed some unique features in comparison with the other seasons: aqueous reactions were more important in the winter, while multiple pathways coexisted in the other seasons. Furthermore, this study confirmed that the PM in Beijing was moderately acidic despite a fully neutralized system. In addition, the acidity variation during pollution episodes displayed different patterns between seasons and was driven by both the variation of aerosol water and chemical compositions. These results provide a new perspective to understand the characteristics and mechanisms of aerosol pollution in Beijing. However, more accurate measurements are necessary for effective air pollution control that depends on the seasonal variation of fine particle formation in Beijing and the surrounding areas.
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http://dx.doi.org/10.1016/j.scitotenv.2017.11.208DOI Listing
April 2018

Temporal and spatial distribution of PM chemical composition in a coastal city of Southeast China.

Sci Total Environ 2017 Dec 29;605-606:337-346. Epub 2017 Jun 29.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.

Rapid economic development and urbanization in China has been concentrated in coastal cities, resulting in haze and photochemical smog issues, especially in the densely-populated Yangtze River Delta. In this study, we explore particulate matter (specifically PM) pollution in a city in Zhejiang Province (Ningbo), chosen to represent a typical, densely-populated urban city with residential and industrial sections. PM samples were collected at five sites in four seasons from Dec. 2012 to Nov. 2013. The annual average PM mass concentration was 53.2±30.4μg/m, with the highest concentration in winter and lowest in summer. Among the five sites, PM concentration was highest in an urban residential site and lowest in a suburban site, due to effects of urbanization and the anthropogenic influences. The chemical components of PM show significant seasonal variation. In addition, secondary transformation was high in Ningbo, with the highest proportion of secondary components found at a suburban site and the lowest at the industrial sites. Ningbo is controlled by five major air masses originating from inland China, from the Bohai Sea, offshore from the southeast, the Yellow Sea, and off the east coast of Korea. The relative contributions of these air masses differ, by season, with the Bohai Sea air mass dominating in winter and spring, the maritime southeast air mass in summer, and the Yellow Sea and coastal Korean air masses dominating in autumn. The continental air mass is associated with a high PM concentration, indicating that it is primarily transports primary emissions. In contrast, the concentration ratios among secondary formed pollutants were higher in the maritime air masses, which suggests that sea breezes control temporal and spatial variations of air pollution over coastal cities.
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http://dx.doi.org/10.1016/j.scitotenv.2017.03.260DOI Listing
December 2017

Effects of continental anthropogenic sources on organic aerosols in the coastal atmosphere of East China.

Environ Pollut 2017 Oct 10;229:350-361. Epub 2017 Jun 10.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.

Although organic compounds in marine atmospheric aerosols have significant effects on climate and marine ecosystems, they have rarely been studied, especially in the coastal regions of East China. To assess the origins of the organic aerosols in the East China coastal atmosphere, PM samples were collected from the atmospheres of the Yellow Sea, the East China Sea, and Changdao Island during the CAPTAIN (Campaign of Air PolluTion At INshore Areas of Eastern China) field campaign in the spring of 2011. The marine atmospheric aerosol samples that were collected were grouped based on the backward trajectories of their air masses. The organic carbon concentrations in the PM samples from the marine and Changdao Island atmospheres were 5.5 ± 3.1 μgC/m and 6.9 ± 2.4 μgC/m, respectively, which is higher than in other coastal water atmospheres. The concentration of polycyclic aromatic hydrocarbons (PAHs) in the marine atmospheric PM samples was 17.0 ± 20.2 ng/m, indicating significant continental anthropogenic influences. The influences of fossil fuels and biomass burning on the composition of organic aerosols in the coastal atmosphere of East China were found to be highly dependent on the origins of the air masses. Diesel combustion had a strong impact on air masses from the Yangtze River Delta (YRD), and gasoline emissions had a more significant impact on the "North China" marine atmospheric samples. The "Northeast China" marine atmospheric samples were most impacted by biomass burning. Coal combustion contributed significantly to the compositions of all of the atmospheric samples. The proportions of secondary compounds increased as samples aged in the marine atmosphere indicating that photochemical oxidation occured during transport. Our results quantified ecosystem effects on marine atmospheric aerosols and highlighted the uncertainties that arise when modeling marine atmospheric PM without considering high spatial resolution source data and meteorological parameters.
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http://dx.doi.org/10.1016/j.envpol.2017.05.015DOI Listing
October 2017

Molecular Characterization of Nitrogen-Containing Organic Compounds in Humic-like Substances Emitted from Straw Residue Burning.

Environ Sci Technol 2017 Jun 25;51(11):5951-5961. Epub 2017 May 25.

Key Laboratory for Urban Habitat Environmental Science and Technology, School of Environment and Energy, Peking University Shenzhen Graduate School , Shenzhen 518055, China.

The molecular composition of humic-like substances (HULIS) in different aerosol samples was analyzed using an ultrahigh-resolution mass spectrometer to investigate the influence of biomass burning on ambient aerosol composition. HULIS in background aerosols were characterized with numerous molecular formulas similar to biogenic secondary organic aerosols. The abundance of nitrogen-containing organic compounds (NOC), including nitrogen-containing bases (N-bases) and nitroaromatics, increased dramatically in ambient aerosols affected by crop residue burning in the farm field. The molecular distribution of N-bases in these samples exhibited similar patterns to those observed in smoke particles freshly emitted from lab-controlled burning of straw residues but were significantly different with those observed from wood burning. Signal intensity of the major N-bases correlated well with the atmospheric concentrations of potassium and levoglucosan. These N-bases can serve as molecular markers distinguishing HULIS from crop residue burning with from wood burning. More nitroaromatics were detected in ambient aerosols affected by straw burning than in fresh smoke aerosols, indicating that many of them are formed in secondary oxidation processes as smoke plumes evolve in the atmosphere. This study highlights the significant contribution of crop residue burning to atmospheric NOC. Further study is warranted to evaluate the roles of NOC on climate and human health.
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http://dx.doi.org/10.1021/acs.est.7b00248DOI Listing
June 2017

Evolution of secondary inorganic and organic aerosols during transport: A case study at a regional receptor site.

Environ Pollut 2016 Nov 9;218:794-803. Epub 2016 Aug 9.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.

Understanding the evolution of aerosols in the atmosphere is of great importance for improving air quality and reducing aerosol-related uncertainties in global climate simulations. Here, a unique haze episode at a regional receptor site near the East China Sea was examined as a case study of the aging process of atmospheric aerosols during transport. An increase in photochemical age from 5 h to more than 25 h and a progressive increase in the fitted mean particle diameter from 70 nm to approximately 300 nm were observed. According to the pollution features and meteorology conditions involved, pollution accumulation (PA), sea breeze (SB), and land breeze (LB) periods were identified. Concentrations of black carbon (BC), hydrocarbon-like organic aerosols (HOA), semi-volatile oxidized organic aerosols (SV-OOA), and nitrate increased by 7-fold up to 39-fold when the air masses passed through Taizhou, a nearby city. In addition, nitrate and SV-OOA dominated the aerosol composition in the urban outflow plumes (52% and 18%, respectively), yet they gradually decreased in concentration during transport. In contrast, sulfate and the low-volatile oxidized organic aerosols (LV-OOA) exhibited more regional footprints and potentially have similar formation mechanisms. The atomic oxygen-to-carbon (O/C) ratio also increased from 0.45 to 0.9, thereby suggesting that rapid formation of highly oxidized secondary organic aerosols (SOA) occurred during transport. Overall, these results provide valuable insight into the evolution of the chemical and physical features of aerosol pollution during transport and also highlight the need for regulatory controls of nitrogen oxides, sulfur dioxide, and VOCs to improve air quality on different scales.
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http://dx.doi.org/10.1016/j.envpol.2016.08.003DOI Listing
November 2016

A novel apatite, Lu5(SiO4)3N:(Ce,Tb), phosphor material: synthesis, structure and applications for NUV-LEDs.

Phys Chem Chem Phys 2016 Jun 11;18(23):15545-54. Epub 2016 May 11.

Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Sciences and Technology, China University of Geosciences, Beijing 100083, China.

The lutetium containing nitride apatite Lu5(SiO4)3N was prepared by a solid state reaction at high temperature for the first time. Rietveld refinement indicated that the Lu5(SiO4)3N compound has a hexagonal space group of P63/m with cell parameters a = b = 9.700 Å and c = 7.238 Å. Additionally, the results revealed that there are two distinct lutetium sites in the Lu5(SiO4)3N host lattice, i.e. a Lu(1) site with nine coordination (Wyckoff site 4f) and a Lu(2) site with seven coordination (Wyckoff site 6h). Furthermore, the ratio of the number of Lu atoms in Lu(1) and Lu(2) sites is 3 : 2. The band gap for Lu5(SiO4)3N was determined to be 4.12 eV based on the density functional theory (DFT). In the Ce(3+) doped Lu5(SiO4)3N:0.03Ce(3+) compound, the emission peak centered at 462 nm was observed with the Commission International de I'Eclairage (CIE) coordinates of (0.148, 0.184), indicating blue-emission. Remarkably, in Ce(3+) and Tb(3+) co-doped Lu4.97-y(SiO4)3N:0.03Ce(3+),yTb(3+) compounds, the color-tunability was observed with increasing Tb(3+) co-doping rate on moving from blue at Tb(3+) = 0.00 to green at Tb = 0.09, due to the energy transfer from Ce(3+) to Tb(3+) ions being matched well with the decay curve results. Under the excitation at 359 nm, the absolute quantum efficiency (QE) for Lu5(SiO4)3N:0.03Ce(3+) was determined to be 42.13%. This phosphor material could be a platform for modeling a new phosphor and application in the solid-state lighting field.
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http://dx.doi.org/10.1039/c6cp01512cDOI Listing
June 2016

A new azaphilone from the entomopathogenic fungus Hypocrella sp.

Nat Prod Res 2015 23;29(21):2000-6. Epub 2015 Mar 23.

a Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University , Fuzhou , Fujian 350002 P.R. China.

This report describes the isolation of a new azaphilone, designated hypocrellone A (2), together with five known compounds (1, 3-6) from a submerged culture of the entomopathogenic fungus Hypocrella sp. (isolate WYTY-21). The absolute stereostructures of the two compounds (1 and 2) were elucidated based on 1D and 2D NMR spectroscopic and mass spectrometric data combined with the data from various chemical transformations. Hypocrellone A (2) and three (3-6) of the five known compounds were cytotoxic to hepatoma cells (cell line BEL-7404); IC50 values ranged from 6.2 to 17.4 μM. At 200 μM, none of the six compounds was toxic to normal human liver cells (cell line HL-7702) or to normal human kidney epithelial cells (cell line HEK-293T).
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http://dx.doi.org/10.1080/14786419.2015.1023199DOI Listing
December 2015

Relationship between urinary nickel and methylation of p15, p16 in workers exposed to nickel.

J Occup Environ Med 2014 May;56(5):489-92

From the Department of Occupational Health (Dr Yang, Mr Chen, Mr Guo, and Mr Wang), School of Public Health, Shanxi Medical University, Taiyuan; and Centers for Disease Control and Prevention (Mr Li and Mr Sun), Taiyuan Iron and Steel (Group) Co Ltd, Shanxi, China.

Objective: The purpose of this study was to investigate the relationship between urinary nickel and methylation of p15, p16 in workers exposed to nickel.

Methods: In this study, 165 nickel-exposed workers and 67 workers without exposure were recruited. The levels of urinary nickel were analyzed using dimethylglyoxime spectrophotometric method. Methylation-specific polymerase chain reaction was used to detect the methylation of p15 and p16.

Results: The median concentration of urinary nickel in the exposed group (4.58 μg/L) was significantly higher than that in the control group (1.78 μg/L; P < 0.01). The rate of methylation of p15 in the exposed group was significantly higher than that in the control group (P = 0.023). The multiple logistic analysis showed that workers having higher urinary nickel were at the higher risk of methylation of p15 (P = 0.024).

Conclusions: The levels of urinary nickel were significantly associated with the methylation of p15.
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http://dx.doi.org/10.1097/JOM.0000000000000168DOI Listing
May 2014

Comparisons of ultrafine and fine particles in their associations with biomarkers reflecting physiological pathways.

Environ Sci Technol 2014 May 11;48(9):5264-73. Epub 2014 Apr 11.

Duke University , Nicholas School of the Environment and Duke Global Health Institute, Durham, North Carolina, United States.

Using a quasi-experimental opportunity offered by greatly restricted air pollution emissions during the Beijing Olympics compared to before and after the Olympics, we conducted the current study to compare ultrafine particles (UFPs) and fine particles (PM2.5) in their associations with biomarkers reflecting multiple pathophysiological pathways linking exposure and cardiorespiratory events. Number concentrations of particles (13.0-764.7 nm) and mass concentrations of PM2.5 were measured at two locations within 9 km from the residence and workplace of 125 participating Beijing residents. Each participant was measured 6 times for biomarkers of autonomic function (heart rate, systolic and diastolic blood pressures), hemostasis (von Willebrand factor, soluble CD40 ligand, and P-selectin), pulmonary inflammation and oxidative stress (exhaled nitric oxide and exhaled breath condensate pH, malondialdehyde, and nitrite), and systemic inflammation and oxidative stress (urinary malondialdehyde and 8-hydroxy-2'-deoxyguanosine, plasma fibrinogen, and white blood cells). Linear mixed models were used to estimate associations of biomarkers with UFPs and PM2.5 measured 1-7 days prior to biomarker measurements (lags). We found that the correlation coefficient for UFPs at two locations (∼ 9 km apart) was 0.45, and at the same location, the correlation coefficient for PM2.5 vs UFPs was -0.18. Changes in biomarker levels associated with increases in UFPs and PM2.5 were comparable in magnitude. However, associations of certain biomarkers with UFPs had different lag patterns compared to those with PM2.5, suggesting that the ultrafine size fraction (≤ 100 nm) and the fine size fraction (∼ 100 nm to 2.5 μm) of PM2.5 are likely to affect PM-induced pathophysiological pathways independently.
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http://dx.doi.org/10.1021/es5006016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015680PMC
May 2014

Proteins differentially expressed in conidia and mycelia of the entomopathogenic fungus Metarhizium anisopliae sensu stricto.

Can J Microbiol 2013 Jul 14;59(7):443-8. Epub 2013 May 14.

Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, People's Republic of China.

Metarhizium anisopliae is a well-characterized entomopathogenic fungus that attacks a variety of insects. Its conidia are involved in its propagation and also in its infection of host insects. To investigate the protein expression profiles and to identify the proteins related to development and pathogenesis, we performed a comparative proteomic analysis of the conidia and mycelia of an M. anisopliae strain (Ma1291). The analysis used 2-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. We detected 898 ± 37 protein spots in conidia and 1072 ± 24 in mycelia of strain Ma1291. A comparison of the 2 protein-expression profiles indicated that only 28% of protein spots were common to both developmental stages. Finally, we identified 30 proteins (19 from conidia and 11 from mycelia). The identified proteins exclusive to conidia were those involved in protective processes, appressorium formation, and degradation of the host cuticle (protease PR1H). The identified proteins exclusive to mycelia included major proteins participating in biosynthetic and energy metabolism, such as UTP-glucose-1-phosphate uridylyltransferase and heat shock protein 70. This research provides the first proteomic analysis of different developmental stages of M. anisopliae, and the results should facilitate clarification of the molecular basis of these epigenetic variations.
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http://dx.doi.org/10.1139/cjm-2013-0096DOI Listing
July 2013

Biological phosphorus removal inhibition by roxarsone in batch culture systems.

Chemosphere 2013 Jun 14;92(1):138-42. Epub 2013 Mar 14.

Department of Civil Engineering, Hefei University of Technology, Hefei 230009, China.

Roxarsone has been extensively used in the feed of animals, which is usually excreted unchanged in the manure and eventually enter into animal wastewater, challenging the biological phosphorus removal processes. Knowledge of its inhibition effect is key for guiding treatment of roxarsone-contaminated wastewater, and is unfortunately keeping unclear. We study the inhibition of roxarsone on biological phosphorus removal processes for roxarsone-contaminated wastewater treatment, in terms of the removal and rates of chemical oxygen demand (COD), phosphate. Results showed that presence of roxarsone considerably limited the COD removals, especially at roxarsone concentration exceeding 40 mg L(-1). Additionally, roxarsone inhibited both phosphorus release and uptake processes, consistent with the phosphate profiles during the biological phosphorus removal processes; whereas, roxarsone is more toxic to phosphorus uptake process, than release function. The results indicated that it is roxarsone itself, rather than the inorganic arsenics, inhibit biological phosphorus removal processes within both aerobic and anaerobic roxarsone-contaminated wastewater treatment.
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http://dx.doi.org/10.1016/j.chemosphere.2013.02.029DOI Listing
June 2013

Fast-track rehabilitation program and conventional care after esophagectomy: a retrospective controlled cohort study.

Support Care Cancer 2013 Mar 30;21(3):707-14. Epub 2012 Aug 30.

Department of Thoracic Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China.

Purpose: The purpose of this article is to evaluate fast-track rehabilitation program and conventional care after esophagectomy using a retrospective controlled cohort study in esophageal cancer patients.

Methods: Fifty-five patients underwent fast-track rehabilitation program and 57 patients underwent conventional care after esophagectomy. Fast-track rehabilitation program was performed to patients who have early movement, epidural analgesia control, fluid infusion volume control and enteral nutrition for early discharge. The other 57 patients underwent conventional care after esophagectomy. The average of hospital stay and complications were calculated in the patients between the two groups.

Results: The median length of hospital stay in the patients was significantly shorter after fast-track rehabilitation program than after conventional care (7.7 vs 14.8 day, P < 0.01). The percentage of patients who developed complications was significantly lower 30 day after fast-track rehabilitation program than after conventional care (29.1 vs 47.4%, P < 0.05). 87.3% in patients of the fast-track rehabilitation program group and 54.4% in those of the conventional care group reported excellent to very good satisfaction with their pain control (P = 0.000).

Conclusions: The fast-track rehabilitation program results in fewer complications, less postoperative pain, a reduction in the hospital length of stay, and quicker return to work and normal activities after esophagectomy.
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http://dx.doi.org/10.1007/s00520-012-1570-0DOI Listing
March 2013

Primary sources and secondary formation of organic aerosols in Beijing, China.

Environ Sci Technol 2012 Sep 29;46(18):9846-53. Epub 2012 Aug 29.

State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, China.

Ambient aerosol samples were collected at an urban site and an upwind rural site of Beijing during the CAREBEIJING-2008 (Campaigns of Air quality REsearch in BEIJING and surrounding region) summer field campaign. Contributions of primary particles and secondary organic aerosols (SOA) were estimated by chemical mass balance (CMB) modeling and tracer-yield method. The apportioned primary and secondary sources explain 73.8% ± 9.7% and 79.6% ± 10.1% of the measured OC at the urban and rural sites, respectively. Secondary organic carbon (SOC) contributes to 32.5 ± 15.9% of the organic carbon (OC) at the urban site, with 17.4 ± 7.6% from toluene, 9.7 ± 5.4% from isoprene, 5.1 ± 2.0% from α-pinene, and 2.3 ± 1.7% from β-caryophyllene. At the rural site, the secondary sources are responsible for 38.4 ± 14.4% of the OC, with the contributions of 17.3 ± 6.9%, 13.9 ± 9.1%, 5.6 ± 1.9%, and 1.7 ± 1.0% from toluene, isoprene, α-pinene, and β-caryophyllene, respectively. Compared with other regions in the world, SOA in Beijing is less aged, but the concentrations are much higher; between the sites, SOA is more aged and affected by regional transport at the urban site. The high SOA loading in Beijing is probably attributed to the high regional SOC background (~2 μg m(-3)). The toluene SOC concentration is high and comparable at the two sites, implying that some anthropogenic components, at least toluene SOA, are widespread in Beijing and represents a major factor in affecting the regional air quality. The aerosol gaseous precursor concentrations and temperature correlate well with SOA, both affecting SOA formation. The significant SOA enhancement with increasing water uptake and acidification indicates that the aqueous-phase reactions are largely responsible SOA formation in Beijing.
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http://dx.doi.org/10.1021/es2042564DOI Listing
September 2012