Publications by authors named "Qingchun Li"

46 Publications

Advantages of interstitial radioactive seed implantation for the treatment of Stage III pancreatic cancer.

J Cancer Res Ther 2021 Jul;17(3):702-706

Department of Ultrasound, Third Hospital of Jilin University, Changchun, China.

Objective: The objective of the study was to identify the advantages of interstitial radioactive seed implantation for the treatment of Stage III pancreatic cancer.

Materials And Methods: Clinical data of 160 patients with pancreatic cancer implanted with radioactive seeds were retrospectively analyzed. Patients were grouped according to tumor size, lymph node metastasis, and tumor invasion to important blood vessels, and survival time statistics were obtained.

Results: The mean postoperative survival time (months) was 24.80 for Stage I, 12.89 for Stage II, 13.51 for Stage III, and 7.49 for Stage IV patients, and the difference between Stage II and Stage III patients was not statistically significant. The efficacy of radioactive seed implantation therapy for pancreatic cancer was strongly associated with tumor size and number of lymph node metastases but not significantly associated with tumor invasion to blood vessels.

Conclusions: Radioactive seed implantation obviously advantageous for the treatment of Stage III pancreatic cancer.
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http://dx.doi.org/10.4103/jcrt.JCRT_803_20DOI Listing
July 2021

Unraveling the dynamic importance of county-level features in trajectory of COVID-19.

Sci Rep 2021 06 22;11(1):13058. Epub 2021 Jun 22.

Zachry Department of Civil and Environmental Engineering, Texas A&M University, 199 Spence St., College Station, TX, 77843, USA.

The objective of this study was to investigate the importance of multiple county-level features in the trajectory of COVID-19. We examined feature importance across 2787 counties in the United States using data-driven machine learning models. Existing mathematical models of disease spread usually focused on the case prediction with different infection rates without incorporating multiple heterogeneous features that could impact the spatial and temporal trajectory of COVID-19. Recognizing this, we trained a data-driven model using 23 features representing six key influencing factors affecting the pandemic spread: social demographics of counties, population activities, mobility within the counties, movement across counties, disease attributes, and social network structure. Also, we categorized counties into multiple groups according to their population densities, and we divided the trajectory of COVID-19 into three stages: the outbreak stage, the social distancing stage, and the reopening stage. The study aimed to answer two research questions: (1) The extent to which the importance of heterogeneous features evolved at different stages; (2) The extent to which the importance of heterogeneous features varied across counties with different characteristics. We fitted a set of random forest models to determine weekly feature importance. The results showed that: (1) Social demographic features, such as gross domestic product, population density, and minority status maintained high-importance features throughout stages of COVID-19 across 2787 studied counties; (2) Within-county mobility features had the highest importance in counties with higher population densities; (3) The feature reflecting the social network structure (Facebook, social connectedness index), had higher importance for counties with higher population densities. The results showed that the data-driven machine learning models could provide important insights to inform policymakers regarding feature importance for counties with various population densities and at different stages of a pandemic life cycle.
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http://dx.doi.org/10.1038/s41598-021-92634-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219723PMC
June 2021

Incidence Density and Predictors of Multidrug-Resistant Tuberculosis Among Individuals With Previous Tuberculosis History: A 15-Year Retrospective Cohort Study.

Front Public Health 2021 28;9:644347. Epub 2021 May 28.

Hangzhou Center for Disease Control and Prevention, Hangzhou, China.

To date, too little attention has been paid to monitoring and estimating the risk of incident multidrug-resistant tuberculosis (MDR-TB) among individuals with a previous tuberculosis history (PTBH). The purpose of this study was to assess the incidence of and risk factors for MDR-TB in those individuals. Between 2005 and 2020, a large, retrospective, population-based cohort study was performed in Hangzhou, China. A multivariable Cox regression model was used to evaluate independent predictors of incident MDR-TB among individuals with PTBH. The incidence density of MDR-TB was 22.6 per 1,000 person-years (95% confidence level and an interval of 20.9-24.3) for individuals with PTBH. The incidence of MDR-TB increased significantly in individuals who • were under 60 years old. • were male. • had a history of direct contact. • came from low-income families. • worked in high-risk occupations. • lived in rural areas. • had a retreatment TB history. • had an unfavorable outcome in their previous treatment ( < 0.05). In addition, we found that the following factors were significantly linked to the MDR-TB risk among individuals with PTBH ( < 0.05): • sociodemographic factors such as the 21-30 and 31-40 year age groups, or a history of direct contact. • clinical factors like passive modes of TB case finding (PMTCF), human immunodeficiency virus infection, unfavorable treatment outcomes, retreated TB history, non-standardized treatment regimens of retreatment TB patients, and duration of pulmonary cavities (DPC). • microbiological factors, such as duration of positive sputum culture. We also found that the 21-30 year age group, low family income, and PMTCF were significantly linked to incident MDR-TB only in males with PTBH, whilst the 41-50 year age group, extended treatment course, and DPC were significantly associated with female MDR-TB only. The incidence of MDR-TB was high, with a higher rate among subjects with a history of direct contact and unfavorable treatment outcomes. There was a gender difference in the incidence density and risk factors of MDR-TB among individuals with PTBH. Long-term monitoring and gender-specific risk-factor modifications should be given to individuals with PTBH.
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http://dx.doi.org/10.3389/fpubh.2021.644347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193499PMC
June 2021

An iron oxide nanoworm hybrid on an interdigitated microelectrode silica surface to detect abdominal aortic aneurysms.

Mikrochim Acta 2021 May 11;188(6):185. Epub 2021 May 11.

Department of Cardiology, China-Japan Union Hospital of Jilin University, Jilin Provincial Key Laboratory for Genetic Diagnosis of Cardiovascular Disease, Jilin Provincial Cardiovascular Research Institute, Changchun, Jilin, 130031, China.

An abdominal aortic aneurysm (AAA) is abnormal swelling in the abdominal aorta and a prevalent life-threatening disease. This research introduces a new interdigitated microelectrode (IDME)-sensing surface modified by iron oxide nanoworms (IONWs) for detecting the AAA biomarker insulin-like growth factor-1 (IGF1). A sandwich pattern was formulated with the IGF1 aptamer and IGFBP1 (IGF binding protein-1) on the IONW-constructed IDME hybrid to identify IGF1. The surface morphology of the IONWs revealed a uniform distribution of worm-like structures (80-100 nm) as confirmed by FESEM and FETEM analyses. Further, the presence of the major elements, Fe and O, was confirmed by EDX and XPS studies. The crystal planes that appeared in the IONW reflect cubic magnetite. IONW-modified IDME attained a limit of detection for IGF1 of 1 fM (3σ) with an aptamer-IGF1-IGFBP1 sandwich. This sandwich with IGFBP1 enhanced the current level at all concentrations of IGF1 and displayed linearity in the range 1 fM to 100 pM with a determination coefficient of R = 0.9373 [y = 3.38221x - 4.79]. Control experiments with complementary aptamer sequences, IGF2 and IGFBP3 did not show notable signal changes, indicating the specific detection of IGF1. This IONW constructed electrode helps to achieve the detection of low amounts of IGF1 and diagnose AAA at the stage prior to rupture.
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http://dx.doi.org/10.1007/s00604-021-04836-8DOI Listing
May 2021

Transcription activation of microRNA-25 by PEA3 augments progression of gastric cancer through suppressing SIK1.

Exp Physiol 2021 Apr 20. Epub 2021 Apr 20.

Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, P.R. China.

New Findings: What is the central question of this study? What is the function of miR-25 in the development of gastric cancer (GC) and the molecular mechanism? What is the main finding and its importance? Polyomavirus enhancer activator protein 3 (PEA3) activates transcription of microRNA-25 and augments progression of GC through suppressing salt-inducible kinase 1 (SIK1) and activating the Wnt/β-catenin pathway.

Abstract: Gastric cancer (GC) is one of the leading causes of cancer-related death worldwide. Aberrant expression of microRNAs (miRNAs) is frequently involved in the pathogenesis of GC. This study aims to determine the functions of miR-25 in GC development and the molecular mechanism. miR-25 was screened to be significantly highly expressed in GC tissues using a miRNA microarray analysis. The subsequent RT-qPCR suggested high-expression profiling of miR-25 in cancer tissues and cells relative to the normal samples. High miR-25 expression indicated poor prognosis and shorter survival time of GC patients. Polyomavirus enhancer activator protein 3 (PEA3) was identified as an upstream transcriptional activator of miR-25. miR-25 directly bound to salt-inducible kinase 1 (SIK1) mRNA. Downregulation of miR-25 suppressed proliferation and invasiveness of GC cells and the growth of xenograft tumors in vivo. These tumor-suppressive effects were strengthened by further silencing of PEA3 but blocked by SIK1 knockdown. The PEA3/miR-25 axis was found to activate the Wnt/β-catenin signaling pathway. To conclude, this study suggested that PEA3 activates miR-25 transcription and regulates GC progression through suppressing SIK1 and activating the Wnt/β-catenin pathway. This study may provide new ideas in GC treatment. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1113/EP089254DOI Listing
April 2021

Decreased amino acids in the brain might contribute to the progression of diabetic neuropathic pain.

Diabetes Res Clin Pract 2021 Jun 1;176:108790. Epub 2021 Apr 1.

Department of Endocrinology and Metabolism, Huashan Hospital, Fudan University, Shanghai 200040, China. Electronic address:

Aims: The pathophysiological alteration of diabetic neuropathic pain (DNP) in brain is unclear. Here we aimed to explore the metabolomic characteristics of brain in rats over the progression of DNP through metabolomic analysis.

Methods: Adult rats were randomly divided into control group and DNP group. Body weight, blood glucose and behavioral assessment of neuropathic pain were measured every week after streptozotocin (STZ) injection. Finally, the brains of 2 rats from control group and 6 rats from DNP group were removed every 4 weeks after STZ injection for metabolomics analysis.

Results: After 4 weeks of STZ-injection, the rats with diabetes developed DNP, which was characterized as mechanical allodynia and thermal nociception. As for metabolomic analysis, differentially expressed metabolites (DE metabolites) showed a dynamic alteration over the development of DNP and affected several KEGG pathways associated with amino acid metabolism. Furthermore, the expression of l-Threonine, l-Methionine, d-Proline, l-Lysine and N-Acetyl-l-alanine were significantly decreased at all time points of DNP group. The amino acids which were precursor of analgesic neurotransmitters were downregulated over the progression of DNP, including l-tryptophan, l-histidine and l-tyrosine.

Conclusions: The impairment of amino acid metabolism in brain might contribute to the progression of DNP through decreasing analgesic neurotransmitters.
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http://dx.doi.org/10.1016/j.diabres.2021.108790DOI Listing
June 2021

Effects of population co-location reduction on cross-county transmission risk of COVID-19 in the United States.

Appl Netw Sci 2021 18;6(1):14. Epub 2021 Feb 18.

Zachry Department of Civil and Environmental Engineering, Texas A&M University, College Station, TX 77843 USA.

The objective of this study is to examine the transmission risk of COVID-19 based on cross-county population co-location data from Facebook. The rapid spread of COVID-19 in the United States has imposed a major threat to public health, the real economy, and human well-being. With the absence of effective vaccines, the preventive actions of social distancing, travel reduction and stay-at-home orders are recognized as essential non-pharmacologic approaches to control the infection and spatial spread of COVID-19. Prior studies demonstrated that human movement and mobility drove the spatiotemporal distribution of COVID-19 in China. Little is known, however, about the patterns and effects of co-location reduction on cross-county transmission risk of COVID-19. This study utilizes Facebook co-location data for all counties in the United States from March to early May 2020 for conducting spatial network analysis where nodes represent counties and edge weights are associated with the co-location probability of populations of the counties. The analysis examines the synchronicity and time lag between travel reduction and pandemic growth trajectory to evaluate the efficacy of social distancing in ceasing the population co-location probabilities, and subsequently the growth in weekly new cases across counties. The results show that the mitigation effects of co-location reduction appear in the growth of weekly new confirmed cases with one week of delay. The analysis categorizes counties based on the number of confirmed COVID-19 cases and examines co-location patterns within and across groups. Significant segregation is found among different county groups. The results suggest that within-group co-location probabilities (e.g., co-location probabilities among counties with high numbers of cases) remain stable, and social distancing policies primarily resulted in reduced cross-group co-location probabilities (due to travel reduction from counties with large number of cases to counties with low numbers of cases). These findings could have important practical implications for local governments to inform their intervention measures for monitoring and reducing the spread of COVID-19, as well as for adoption in future pandemics. Public policy, economic forecasting, and epidemic modeling need to account for population co-location patterns in evaluating transmission risk of COVID-19 across counties.
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http://dx.doi.org/10.1007/s41109-021-00361-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7891476PMC
February 2021

Disparate patterns of movements and visits to points of interest located in urban hotspots across US metropolitan cities during COVID-19.

R Soc Open Sci 2021 Jan 13;8(1):201209. Epub 2021 Jan 13.

Zachry Department of Civil and Environmental Engineering, Texas A&M University, 199 Spence Street, College Station, TX 77843-3112, USA.

We examined the effect of social distancing on changes in visits to urban hotspot points of interest. In a pandemic situation, urban hotspots could be potential superspreader areas as visits to urban hotspots can increase the risk of contact and transmission of a disease among a population. We mapped census-block-group to point-of-interest (POI) movement networks in 16 cities in the United States. We adopted a modified coarse-grain approach to examine patterns of visits to POIs among hotspots and non-hotspots from January to May 2020. Also, we conducted chi-square tests to identify POIs with significant flux-in changes during the analysis period. The results showed disparate patterns across cities in terms of reduction in hotspot POI visitors. Sixteen cities were divided into two categories using a time series clustering method. In one category, which includes the cities of San Francisco, Seattle and Chicago, we observed a considerable decrease in hotspot POI visitors, while in another category, including the cities of Austin, Houston and San Diego, the visitors to hotspots did not greatly decrease. While all the cities exhibited overall decreased visitors to POIs, one category maintained the proportion of visitors to hotspot POIs. The proportion of visitors to some POIs (e.g. restaurants) remained stable during the social distancing period, while some POIs had an increased proportion of visitors (e.g. grocery stores). We also identified POIs with significant flux-in changes, indicating that related businesses were greatly affected by social distancing. The study was limited to 16 metropolitan cities in the United States. The proposed methodology could be applied to digital trace data in other cities and countries to study the patterns of movements to POIs during the COVID-19 pandemic.
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http://dx.doi.org/10.1098/rsos.201209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890478PMC
January 2021

Identification of differentially expressed miRNAs in serum extracellular vesicles (EVs) of Kazakh sheep at early pregnancy.

Reprod Domest Anim 2021 May 9;56(5):713-724. Epub 2021 Mar 9.

College of Animal Science and Technology, Shihezi University, Shihezi, China.

MiRNAs-containing extracellular vesicles (EVs) possess the unique function of mediating intercellular communication and participating in many biological processes such as post-transcriptional gene regulation of embryo implantation and placental development. In the present study, Illumina small-RNA sequencing was used to identify differentially expressed (DE) miRNAs in serum EVs of pregnant (P) and non-pregnant (NP) Kazakh sheep at Day 17 from mating. The specifically and differentially expressed miRNAs at early pregnancy in sheep were verified by using RT-PCR. The target genes of DE miRNAs were predicted by bioinformatics software, and the functional and pathway enrichment analysis was performed on Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) terms. A total of 562 miRNAs (210 novel miRNAs) were identified by sequencing, of which 57 miRNAs were differentially expressed, 49 were up-regulated, 8 were down-regulated and 22 novel miRNAs were specifically expressed in the pregnant sheep. Eight highly expressed known miRNA (miR-378-3p, miR-320-3p, miR-22-3p, let-7b, miR-423-3p, miR-221, miR-296-3p, miR-147-3p) in pregnant group were down-regulated in the control group. miRNAs-containing pregnancy-related terms and regulatory pathways regulation were enriched using both GO and KEGG analyses. Moreover, we also envisioned a miRNA-mRNA interaction network to understand the function of miRNAs involved in the early pregnancy serum regulatory network. The results of RT-PCR verification confirmed the reliability of small-RNA sequencing. Among them, miR-22-3p and miR-378-3p were significantly differentially expressed (DE) between pregnant sheep and non-pregnant group (p <  0.01). The site at which oar-miR-22-3p binds MAPK3 was determined with a dual-luciferase system. This is the first integrated analysis of the expression profiles of EV-miRNAs and their targets during early pregnancy in ewes. These data identify key miRNAs that influence the implantation of sheep in the early stage of pregnancy, and provide theoretical basis for further molecular regulatory mechanisms research.
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http://dx.doi.org/10.1111/rda.13910DOI Listing
May 2021

The anti-angiogenesis role of FBXW7 in diabetic retinopathy by facilitating the ubiquitination degradation of c-Myc to orchestrate the HDAC2.

J Cell Mol Med 2021 Feb 25;25(4):2190-2202. Epub 2020 Dec 25.

Aier Eye Hospital of Wuhan University, Wuhan, China.

Diabetic retinopathy (DR) is the most prevalently occurring microvascular complication in diabetic patients that triggers severe visual impairments. The anti-angiogenesis role of FBXW7 has been identified in breast cancer. Therefore, this study intends to decipher the mechanism of FBXW7 in angiogenesis of DR. DR model was induced on mice using high-glucose (HG) and high-fat diet, and retinal microvascular endothelial cells (RMECs) isolated from normal mice were induced with HG, followed by evaluation of FBXW7, Ki67, HIF-1α and VEGF expression by immunofluorescence, immunohistochemistry or Western blot analysis. After gain- and loss-of-function assays in normal and DR mice, angiogenesis was assessed by CD31 fluorescence staining and Western blot analysis. After ectopic expression and silencing experiments in HG-induced RMECs, RMEC proliferation, migration and angiogenesis were, respectively, determined by EdU, Transwell and in vitro angiogenesis assays. The impact of FBXW7 on the ubiquitination of c-Myc was studied by cycloheximide chase assay and proteasome inhibition, and the binding of c-Myc to HDAC2 promoter by dual-luciferase reporter gene experiment. DR mice and HG-induced RMECs possessed down-regulated FBXW7 and up-regulated Ki67, HIF-1α and VEGF. Silencing FBXW7 enhanced angiogenesis in normal mouse retinal tissue, but overexpressing FBXW7 or silencing c-Myc diminished angiogenesis in DR mouse retinal tissue. Overexpressing FBXW7 or silencing c-Myc depressed proliferation, migration and angiogenesis in HG-induced RMECs. FBXW7 induced c-Myc ubiquitination degradation, and c-Myc augmented HDAC2 expression by binding to HDAC2 promoter. Conclusively, our data provided a novel sight of anti-angiogenesis role of FBXW7 in DR by modulating the c-Myc/HDAC2 axis.
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http://dx.doi.org/10.1111/jcmm.16204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882985PMC
February 2021

Impact of different tuberculosis history at the onset of future multidrug-resistant tuberculosis: A large, retrospective, population-based cohort study.

J Glob Antimicrob Resist 2021 03 24;24:158-168. Epub 2020 Dec 24.

Hangzhou Center for Disease Control and Prevention, Hangzhou 310021, China. Electronic address:

Objectives: The impact of tuberculosis (TB) history on the risk of multidrug-resistant tuberculosis (MDR-TB) is not yet fully understood. We aimed to identify the impact of different TB history at the onset of future MDR-TB.

Methods: A large, retrospective, population-based cohort study was performed between 2005 and 2019. A multivariable Cox model was used to evaluate independent risk factors for MDR-TB for individuals with different previous TB history (PTBH), such as newly diagnosed TB history (NDTH) and re-treated TB history (RTH).

Results: Overall, 12 172 individuals with PTBH were included in this study. The main impacts of different PTBH at the onset of future MDR-TB were as follows: (a) low family income, high-risk occupation, TB patients with severe infection, extended or shortened treatment course, 2H3R3Z3E3/4H3R3 and frequency of sputum culture were significantly linked to incident MDR-TB only in individuals with NDTH (P < 0.05); (b) passive mode of TB case finding, individualised treatment regimens, 3HRZES/6HRE, duration of pulmonary cavities, excellent frequency of chest X-ray examination and duration of negative sputum smear were significantly associated with incident MDR-TB only in individuals with RTH (P < 0.05); (c) age <60 years, history of direct contact, human immunodeficiency virus (HIV) infection, unsuccessful treatment and duration of positive sputum culture were related to incident MDR-TB in both categories of PTBH individuals (P < 0.05).

Conclusion: Early and differential surveillances, assessments and interventions for reducing the risk of MDR-TB among individuals with different PTBH play a key role.
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http://dx.doi.org/10.1016/j.jgar.2020.12.001DOI Listing
March 2021

Time to sputum culture conversion and its predictors among patients with multidrug-resistant tuberculosis in Hangzhou, China: A retrospective cohort study.

Medicine (Baltimore) 2020 Dec;99(50):e23649

SJTU-Yale Joint Center for Biostatistics and Data Science, Shanghai Jiao Tong University (SJTU), Shanghai, China.

The objective is to investigate the time to initial sputum culture conversion (SCC) and its predictors among multidrug-resistant tuberculosis (MDR-TB) patients in Hangzhou, China.A retrospective cohort study was conducted among patients who initiated MDR-TB treatment from 2011 to 2015 in Hangzhou, China. Successful achievement of initial SCC was defined as 2 consecutive negative cultures taken at least 30 days apart after initiation of treatment of MDR-TB. Successful treatment outcomes included being cured and completing treatment, while poor treatment outcomes included treatment failure, loss to follow-up, and death. Time to initial SCC was analyzed using the Kaplan-Meier method, and Cox proportional hazards regression was used to identify predictors of SCC.Among 384 patients enrolled with MDR-TB, 359 (93.5%) successfully achieved initial SCC after a median of 85 days (interquartile range, 40-112 days). A higher rate of SCC was observed in participants with successful treatment outcomes than those with poor treatment outcomes (P<.01). Multivariate analysis showed that age 25 to 64 years (compared with age<25; adjusted odds ratio [AOR], 0.7; 95% confidence interval [CI], 0.5-0.9; P < .01), age ≥65 years (compared with age < 25; AOR, 0.5; 95% CI, 0.3-0.8; P < .01), and household registration in Hangzhou (compared with non-Hangzhou registration; AOR, 1.3; 95% CI, 1.0-1.5; P < .05) were found to be associated with SCC.Although high SCC and treatment success rates were observed among MDR-TB patients in Hangzhou, the prolonged duration to initial SCC underscores the importance of emphasizing measures for infection control. A new policy of shifting outpatient treatment to inpatient treatment in China may reduce the risk of transmission from patients in the time window prior to SCC.
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http://dx.doi.org/10.1097/MD.0000000000023649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738096PMC
December 2020

Comparison of small-diameter decellularized scaffolds from the aorta and carotid artery of pigs.

Int J Artif Organs 2021 May 28;44(5):350-360. Epub 2020 Sep 28.

Department of Plastic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

Aim: Tissue-specific extracellular matrix promotes tissue regeneration and repair. We aimed to identify the optimal decellularized matrices for tissue-engineered vascular graft (TEVG).

Methods: Decellularized aorta of fetal pigs (DAFP,  = 6, group A), decellularized aorta of adult pigs (DAAP,  = 6, group B), and decellularized carotid artery of adult pigs (DCAP,  = 6, group C) were prepared. Scaffolds were compared using histology and ultrastructure. Endothelial cell (EC) and myofibroblast (MFB) infiltration assessments were performed in vitro. Cell infiltration was measured in vivo. Biomechanical properties were also determined.

Results: Almost original cells were removed by the acellularization procedure, while the construction of the matrix basically remained. In vitro, monolayer ECs and multi-layer MFBs were formed onto the internal surface of the specimens after 3 weeks. In vivo, cell infiltration in group A significantly increased at the 6th and 8th week when compared with groups B and C ( < 0.01). The infiltrated cells were mainly MFBs and a few CD4+ T-lymphocytes/macrophages in the specimens. Groups A and B showed greater axial compliance than group C ( < 0.01).

Conclusion: DAFP was the most suitable for use as a small-caliber vascular graft.
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http://dx.doi.org/10.1177/0391398820959350DOI Listing
May 2021

CircHIPK3/miR-876-5p/PIK3R1 axis regulates regulation proliferation, migration, invasion, and glutaminolysis in gastric cancer cells.

Cancer Cell Int 2020 13;20:391. Epub 2020 Aug 13.

Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, No. 126, Xiantai Street, Changchun, 130031 Jilin China.

Background: Circular RNAs (circRNAs) are a new group of non-coding RNAs that play vital roles in cancer occurrence, including gastric cancer (GC). Nevertheless, the role and underlying regulatory mechanisms of circHIPK3 in GC remain unclear.

Methods: The expression levels of circHIPK3, miR-876-5p, and phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) were estimated by real-time quantitative polymerase chain reaction (RT-qPCR) assay. The proliferation, migration, and invasion of GC cells were determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazol-3-ium bromide (MTT) and transwell assay. Glutaminolysis of GC cells was assessed by measuring glutamine, glutamate, and α-ketoglutarate levels. The western blot was employed to examine the related-protein expression. The association between miR-876-5p and circHIPK3 or PIK3R1 was predicted and affirmed by bioinformatics database starBase v2.0 and dual-luciferase reporter assay, respectively. Eventually, the xenograft experiment was used to assess the role of circHIPK3 silencing in vivo.

Results: CircHIPK3 was upregulated in GC tissues and cells compared with controls, and circHIPK3 was more resistance to RNase R than linear homeodomain interacting protein kinase 3 (HIPK3) mRNA. Silencing of circHIPK3 inhibited GC cells proliferation, migration, invasion, and glutaminolysis as well as tumor tumorigenic ability. Moreover, we also found that miR-876-5p, interacted with PIK3R1, was a target gene of circHIPK3. CircHIPK3 silencing induced effects on GC cells were abolished by silencing of miR-876-5p. In addition, upregulation of PIK3R1 inversed miR-876-5p overexpression-induced effects on GC cells.

Conclusion: The circHIPK3 mediated the proliferation, migration, invasion, and glutaminolysis of GC cells partly through regulation of miR-876-5p/PIK3R1 axis by the mechanism of competing endogenous RNAs (ceRNA), indicating circHIPK3 was a GC-associated circRNA that promoted GC development.
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http://dx.doi.org/10.1186/s12935-020-01455-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7427081PMC
August 2020

Nomogram for individualized prediction of incident multidrug-resistant tuberculosis after completing pulmonary tuberculosis treatment.

Sci Rep 2020 08 13;10(1):13730. Epub 2020 Aug 13.

Division of Infectious Diseases, Hangzhou Center for Disease Control and Prevention, 568 Mingshi Road, Hangzhou, 310021, China.

The purposes of this study were to construct a comprehensive nomogram for providing a simple, precise and personalized prediction of incident multidrug-resistant tuberculosis (MDR-TB) after completing pulmonary tuberculosis treatment (CPTBT). A matched case-control study (1:2 ratios) was performed between 2005 and 2018. A multivariable Cox regression analysis was used to evaluate independent predictors of incident MDR-TB after the CPTBT. A comprehensive nomogram was developed based on the multivariable Cox model. Overall, 1, 836 participants were included in this study. We developed and validated a simple-to-use nomogram that predicted the individualized risk of incident MDR-TB by using 10 parameters after the CPTBT. The concordance index of this nomogram was 0.833 [95% confidence interval (CI) 0.807-0.859] and 0.871 (95% CI 0.773-0.969) for the training and validation sets, respectively, which indicated adequate discriminatory power. The calibration curves for the risk of incident MDR-TB showed an optimal agreement between nomogram prediction and actual observation in the training and validation sets, respectively. The high sensitivity and specificity of nomogram was indicated by using a receiver operating characteristic curve analysis. Through this clinic tool, TB control executives could more precisely monitor, estimate and intervene the risk of incident MDR-TB among individuals with CPTBT.
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http://dx.doi.org/10.1038/s41598-020-70748-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426812PMC
August 2020

Treatment outcomes of multidrug-resistant tuberculosis in Hangzhou, China, 2011 to 2015.

Medicine (Baltimore) 2020 Jul;99(30):e21296

SJTU-Yale Joint Center for Biostatistics and Data Science, Shanghai Jiao Tong University, Shanghai, China.

Treatment of multidrug-resistant tuberculosis (MDR-TB) is challenging. More research is needed to understand treatment outcomes and associated factors.A retrospective cohort study was conducted to assess trends and predictors of treatment success among 398 MDR-TB and extensively drug resistant TB patients who started treatment in 2011 to 2015 in Hangzhou, China. Sociodemographic and clinical characteristic data were obtained from the national reporting database. Chi-square test for trend was used to evaluate changes in treatment success rates over the study years, and Cox regression analysis was used to identify predictors for poor treatment outcomes.The treatment success rate was 76% (301/398) for all participants, 77% (298/387) for MDR-TB cases and 27% (3/11) for extensively drug-resistant tuberculosis -TB cases. Treatment success increased significantly from 66% among patients who started treatment in 2011 to 85% in 2015 (P < .01). Of the 97 (24.4%) patients with unsuccessful treatment outcomes, 10 (2.5%) died, 64 (16.1%) failed treatment, and 23 (5.8%) were lost to follow-up. Patients who started treatment in 2013 to 2015 were less likely to have unsuccessful outcomes than those who started in 2011-2012 (adjusted odds ratio [AOR] 0.4, 95% confidence interval [CI] 0.3-0.6), patients ≥25 years were more likely to have unsuccessful outcomes than younger patients (AOR 1.6, 95% CI 1.3-2.1), and cases with kanamycin resistance was associated with three times the odds of having unsuccessful outcomes than kanamycin-susceptible cases (AOR 3.0, 95% CI 1.5-5.8).With proper case management of MDR-TB, patients can achieve a high treatment success rate. Hangzhou's program offers clinical evidence that can be used to inform MDR-TB programs elsewhere in China and abroad.
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http://dx.doi.org/10.1097/MD.0000000000021296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387009PMC
July 2020

Circ_0008035 contributes to cell proliferation and inhibits apoptosis and ferroptosis in gastric cancer via miR-599/EIF4A1 axis.

Cancer Cell Int 2020 16;20:84. Epub 2020 Mar 16.

1Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, No.126, Xiantai Street, Changchun, 130031 Jilin China.

Background: Currently, multiple circular RNAs (circRNAs) have been verified to act as essential regulators in the progression of gastric cancer (GC). We aimed to investigate the role of circ_0008035 in GC progression.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was utilized to measure the expression of circ_0008035 and miR-599. 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2--tetrazolium bromide (MTT) assay was employed to evaluate cell proliferation and ferroptosis. Western blot assay was performed to measure the levels of cyclin D1, proliferating cell nuclear antigen (PCNA) and eukaryotic initiation factor 4A1 (EIF4A1). Flow cytometry analysis was conducted to assess cell apoptosis. The iron accumulation, lipid peroxidation and mitochondrial membrane potential were examined by relevant kits. Dual-luciferase reporter assay was conducted to determine the targeting relationship between miR-599 and circ_0008035 or EIF4A1. A murine xenograft model was established to investigate the function of circ_0008035 in vivo.

Results: Circ_0008035 was up-regulated in GC tissues and cells. Silencing of circ_0008035 repressed cell proliferation and induced cell apoptosis and ferroptosis in GC cells. Circ_0008035 acted as a sponge of miR-599. The effects of circ_0008035 knockdown on GC cell proliferation, apoptosis and ferroptosis were abolished by miR-599 inhibition. EIF4A1 was confirmed to be a target gene of miR-599. Circ_0008035 knockdown inhibited EIF4A1 expression by targeting miR-599. Moreover, the suppressive role of circ_0008035 deficiency in GC progression could be restored by EIF4A1. Additionally, circ-0008035 knockdown hampered tumorigenesis in vivo.

Conclusion: Circ_0008035 promoted GC cell growth and repressed apoptosis and ferroptosis by up-regulating EIF4A1 through sponging miR-599.
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http://dx.doi.org/10.1186/s12935-020-01168-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7076943PMC
March 2020

Modeling of inter-organizational coordination dynamics in resilience planning of infrastructure systems: A multilayer network simulation framework.

PLoS One 2019 13;14(11):e0224522. Epub 2019 Nov 13.

Zachry Department of Civil and Environmental Engineering, Urban Resilience, Networks, and Informatics Lab, Texas A&M University, College Station, Texas, United States of America.

This paper proposes and tests a multilayer framework for simulating the network dynamics of inter-organizational coordination among interdependent infrastructure systems (IISs) in resilience planning. Inter-organizational coordination among IISs (such as transportation, flood control, and emergency management) would greatly affect the effectiveness of resilience planning. Hence, it is important to examine and understand the dynamics of coordination in networks of organizations within and across various systems in resilience planning. To capture the dynamic nature of coordination frequency and the heterogeneity of organizations, this paper proposes a multilayer network simulation framework enabling the characterization of inter-organizational coordination dynamics within and across IISs. In the proposed framework, coordination probabilities are utilized to approximate the varying levels of collaboration among organizations. Based on these derived collaborations, the simulation process perturbs intra-layer or inter-layer links and unveils the level of inter-organizational coordination within and across IISs. To test the proposed framework, the study examined a multilayer collaboration network of 35 organizations from five infrastructure systems within Harris County, Texas, based on the data gathered from a survey in the aftermath of Hurricane Harvey. The results indicate that prior to Hurricane Harvey: (1) coordination among organizations across different infrastructure systems is less than the coordination within the individual systems; (2) organizations from the community development system had a low level of coordination for hazard mitigation with organizations in flood control and transportation systems; (3) achieving a greater level of coordination among organizations across infrastructure systems is more difficult and would require a greater frequency of interaction (compared to within-system coordination). The results show the capability of the proposed multilayer network simulation framework to examine inter-organizational coordination dynamics at the system level (e.g., within and across IISs). The assessment of inter-organizational coordination within and across IISs sheds light on important organizational interdependencies in IISs and leads to recommendations for improving the resilience planning process.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224522PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6853286PMC
March 2020

The Preventive Effect of IL-1beta Antagonist on Diabetic Peripheral Neuropathy.

Endocr Metab Immune Disord Drug Targets 2020 ;20(5):753-759

Department of Endocrinology and Metabolism, Huashan Hospital, Fudan University 12# Middle Wulumuqi Road, Shanghai, China.

Objective: To investigate the relationship between Interleukin-1beta (IL-1beta) and diabetic peripheral neuropathy (DPN) using animal models.

Methods: The rat model of diabetic neuropathy was induced by intraperitoneal injection of a single dose of streptozotocin (STZ) at 65mg/kg. Diabetic rats were randomly divided into two groups (10 each), one treated with 0.9% saline (DMS group) and the other with interleukin-1 receptor antagonist (IL-1RA) at 50mg/kg (DMI group) twice a day for 5 weeks. Ten normal rats matched for weight, age and sex served as normal controls (Con group) and were treated with saline. Morphologic studies of sciatic nerves were achieved using light and transmission electron microscopy.

Results: Transmission electron microscopy of the sciatic nerve showed the ultrastructure of myelin and the axon in the IL-1RA group was highly protected compared to diabetic controls.

Conclusion: High levels of circulating IL-1beta may be associated with the risk of DPN and anti-IL-1 treatment may provide a potential strategy for the prevention of diabetic neuropathy.
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http://dx.doi.org/10.2174/1871530319666191022114139DOI Listing
April 2021

HIV incidence and cohort retention among men who have sex with men in Hangzhou, China: A prospective cohort study.

Medicine (Baltimore) 2019 Oct;98(40):e17419

Department of Biostatistics, Yale School of Public Health, New Haven, Connecticut.

Prospective cohort studies have been conducted to estimate HIV incidence among men who have sex with men (MSM) in first-line megacities cities (>10 million residents) in China, but few in the second-line large- or middle-size cities. This study was to investigate HIV incidence and cohort retention among MSM in a second-line city Hangzhou in eastern China.A total of 523 HIV-seronegative MSM were recruited during September 2014 to September 2015, and were followed up prospectively at 3, 6, 9, and 12 months. Questionnaire interviews were conducted, and laboratory tests were performed to evaluate baseline syphilis infection and HIV seroconversions. Chi-square test and logistic regression model were used to identify factors associated with cohort retention rate and syphilis prevalence.Of 523 participants, 137 (26.2%) completed 6-month follow-up, and use of Internet for recruiting study participants (vs other recruitments: adjusted odds ratio [AOR] = 0.5; 95% confidence interval [CI]: 0.3-0.8) and being homosexual (vs heterosexual or bisexual: AOR = 0.6; 95% CI: 0.4-0.9) were associated with lower cohort retention. The overall HIV incidence during 12 months of follow-up was 6.6 per 100 person-years (95% CI: 3.4-9.8/100 PY). The prevalence of syphilis at baseline was 6.5% (95% CI: 4.4%-8.6%), and disclosing sexual orientation (AOR = 0.4, 95% CI: 0.2-0.9) was associated with lower risk of syphilis infection.HIV is spreading rapidly among MSM in the second-line Chinese city. Effective interventions are needed to target this population in both first-line megacities and second-line large and middle-size cities.
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http://dx.doi.org/10.1097/MD.0000000000017419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783165PMC
October 2019

Interpretation of adverse reactions and complications in Chinese expert consensus of Iodine-125 brachytherapy for pancreatic cancer.

J Cancer Res Ther 2019 ;15(4):751-754

Department of Gastrointestinal Surgical, Third Hospital of Jilin University, Changchun, China.

Owing to the location of the pancreas and its complex anatomical relationship, it is difficult to perform radioactive Iodine-125 seed implantation in patients with pancreatic cancer as it can cause surgical side effects and further complications. To standardize the procedure of radioactive Iodine-125 seed implantation in the treatment of pancreatic cancer and reduce the occurrence of adverse reactions and complications during and after operation, the Chinese Medical Doctor Association of Radioactive Seed Implantation Technology Expert Committee, Committee of Minimally Invasive Therapy in Oncology, Chinese Anti-Cancer Association, and the Radioactive Seed Therapy Branch organized and helped establish an expert consensus in China regarding radioactive Iodine-125 seed implantation in the treatment of pancreatic cancer. This article aims at interpreting the adverse reactions and complications after the implantation of radioactive seeds.
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http://dx.doi.org/10.4103/jcrt.JCRT_884_18DOI Listing
February 2020

[Association of EphA2 gene polymorphisms with susceptibility to age-related cataract among ethnic Han Chinese from Hubei].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2019 Apr;36(4):380-383

Department of ENT, Head and Neck Surgery, the Second Affiliated Hospital of Hubei College of Science and Technology, Xianning, Hubei 437100, China.

Objective: To assess the association of Eph-receptor tyrosinekinase-type A2 (EphA2) gene polymorphisms with susceptibility to age-related cataract (ARC) among ethnic Han Chinese from Hubei Province.

Methods: 280 patients with cortical ARC and 200 healthy controls were recruited. Polymorphisms at four loci (rs3768293, rs3754334, rs477558 and rs7548209) of the EphA2 gene were detected by PCR-restriction fragment length polymorphism (PCR-RELP) assay. Allelic and genotypic frequencies of the two groups were compared.

Results: Compared with the control group, the AA genotype of rs3768293 locus was more common, while the AC genotype was much rarer (P< 0.05). No significant difference was found in allelic and genotypic frequencies of rs3754334 locus between the two groups (P> 0.05). The AA genotype of the rs477558 locus was more common in the patient group, while the AG genotype was much rarer (P< 0.05). The genotype GG of the rs7548209 locus was more common in the patient group, while the CG genotype was rarer (P< 0.05), though no significant difference in allelic or haplotypic frequencies between the two groups (P> 0.05).

Conclusion: Polymorphisms of rs477558, rs7548209 and rs3768293 loci of the EphA2 gene are associated with susceptibility to ARC among ethnic Han Chinese from Hubei province.
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http://dx.doi.org/10.3760/cma.j.issn.1003-9406.2019.04.022DOI Listing
April 2019

Quantitative analysis of corneal nerve fibers in type 2 diabetics with and without diabetic peripheral neuropathy: Comparison of manual and automated assessments.

Diabetes Res Clin Pract 2019 May 30;151:33-38. Epub 2019 Mar 30.

Department of Endocrinology, Shanghai Jing'an District Central Hospital, Fudan University, Shanghai 200040, China. Electronic address:

Aims: To examine and compare fully-automated and manually measured corneal nerve fiber parameters in type 2 diabetes mellitus (T2DM) patients with and without diabetic peripheral neuropathy (DPN).

Methods: A total of 128 T2DM subjects and 24 healthy controls underwent neuropathy assessment and bilateral corneal confocal microscopy (CCM). Five representative nerve fiber images were selected for each participant and analyzed manually and with fully-automated software. Corneal nerve fiber length (CNFL), branch density (CNBD), and fiber density (CNFD) were examined.

Results: Manual and full-automated methods for the whole cohort were significantly positive correlated for CNFL, CNBD and CNFD (r = 0.818, 0.845, 0.457, all P < 0.001). Analysis of agreement between the two measurements using Bland-Altman method showed a bias of 2.05 mm/mm (95% limits of agreement: -2.03 mm/mm, 6.13 mm/mm), 1.62 no./mm (95% limits of agreement: -17.92 no./mm, 21.17 no./mm), and 16.0 no./mm (95% limits of agreement: -0.14 no./mm, 32.14 no./mm) for CNFL, CNBD and CNFD respectively. A progressive decrease in manual and full-automated CNFL, CNBD and CNFD accompanied with the occurrence of DPN, The fully-automated method slightly underestimated corneal nerve fiber parameters.

Conclusions: This study demonstrated strong correlations between manual and fully-automated CNFL and CNBD, but not CNFD. Fully-automated corneal nerve fiber parameter quantification may be a fast, objective way to detect DPN.
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http://dx.doi.org/10.1016/j.diabres.2019.03.039DOI Listing
May 2019

Permanent Iodine-125 Seed Implantation for the Treatment of Nonresectable Retroperitoneal Malignant Tumors.

Technol Cancer Res Treat 2019 01;18:1533033819825845

1 Department of Gastrointestinal Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.

Purpose: This study aimed to investigate the outcomes of permanent Iodine-125 (I) radiotherapy for patients with unresectable retroperitoneal malignant tumor.

Methods: Twenty-six patients with retroperitoneal malignant tumors were implanted with I seeds under ultrasound guidance from June 2012 to June 2015. The patients were then followed up for 3 to 36 months after the implantation. During the follow-up, pain relief, control of tumor growth, over survival rate, and complications were evaluated.

Results: Most of the patients (90%, 24/26) suffered from mild to severe pain before I seed treatment. After 1-month treatment, 16 patients had 100% pain relief, 4 patients had at least 50% pain relief, and 4 patients had no response, showing 83.3% of pain relief response. Results of computed tomography scan after 2-month I treatment indicated that 3 patients had complete remission in the tumor size, 20 patients had partial remission in tumor size, 2 patients were stable, and 1 patient had progressive disease, accounting for 88.4% response in tumor size remission. The median survival of the 26 patients was 11 months. The 1-year and 2-year overall survival rates were 46% and 27%, respectively. The median survival of the 5 patients with pancreatic cancer was 9.4 months. None of the patients had any severe complications.

Conclusions: I implantation could effectively relieve the pain in the patients with advanced primary or metastatic retroperitoneal malignant tumors and suppress local tumor progress.
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http://dx.doi.org/10.1177/1533033819825845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373988PMC
January 2019

The impact on glycemic control through progressive resistance training with bioDensity in Chinese elderly patients with type 2 diabetes: The PReTTy2 (Progressive Resistance Training in Type 2 Diabetes) Trial.

Diabetes Res Clin Pract 2019 Apr 16;150:64-71. Epub 2019 Feb 16.

Department of Endocrinology and Metabolism, Huashan Hospital, Fudan University, Shanghai, People's Republic of China. Electronic address:

Aims: To evaluate the effects of a novel, low-volume, high-intensity Progressive Resistance Training (PRT) technique on blood glucose control in elderly Chinese patients with Type 2 Diabetes.

Materials And Methods: The PReTTy2 trial enrolled 300 male and female patients with Type 2 Diabetes in a randomized resistance training program with the bioDensity™ technique. 100 were control patients with no training intervention and 200 had resistance training. Anthropometry, biochemical parameters, HbA1c and fasting plasma glucose (FPG) were measured at baseline, 3-month and 6-month intervals.

Results: 265 patients completed the study with no adverse events. There were no statistically significant differences in HbA1c for all patients, control and PRT groups, at baseline (p = 0.60), 3 months (p = 0.42) and 6-months (p = 0.45). Subgroup analysis with baseline HbA1c > 7.5% (58 mmol/mol), showed statistically significant differences in HbA1c and FPG between groups at 6 months (p < 0.05). All PRT group patients had statistically significant differences from baseline at 6 months for HDL (1.25 + 0.32 vs. 1.17 + 0.26 mmol/L, p < 0.001), LDL (3.23 ± 0.89 vs. 2.93 ± 0.80 mmol/L, p < 0.001) and total cholesterol (4.97 ± 1.22 vs. 4.58 ± 1.03 mmol/L, p < 0.001).

Conclusions: PRT improves glycemic indices in elderly patients with Type 2 Diabetes with poor glucose control as an adjunct to diet and medication. Progressive Resistance Training with bioDensity™ is feasible, safe and effective in elderly patients with Type 2 Diabetes.
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http://dx.doi.org/10.1016/j.diabres.2019.02.011DOI Listing
April 2019

Low serum phosphate and magnesium levels are associated with peripheral neuropathy in patients with type 2 diabetes mellitus.

Diabetes Res Clin Pract 2018 Dec 28;146:1-7. Epub 2018 Sep 28.

Department of Endocrinology and Metabolism, Huashan Hospital, Fudan University, Shanghai 200040, China. Electronic address:

Aims: To determine the relationship of serum phosphate, serum magnesium and peripheral nerve function in patients with type 2 diabetes mellitus (T2DM).

Methods: A total of 254 patients diagnosed with T2DM were included. Peripheral nerve function was evaluated by nerve conduction study with the use of electromyography. Composite z scores of conduction velocity, latency, and amplitude were constructed, respectively. Demographic, medical and laboratory data including serum phosphate and magnesium were collected.

Results: Serum phosphate and serum magnesium levels were significantly lower in patients with diabetic peripheral neuropathy (DPN) (P < 0.01). And the percentages of DPN patients were lower in high tertile of serum phosphate and serum magnesium (P < 0.05). Furthermore, composite z score of conduction velocity (CV) (P = 0.012) were positively associated with serum phosphate levels and the composite z score of amplitude (P < 0.001) and CV (P = 0.041) were positively associated with serum magnesium levels. After adjusting potential related factors (age, gender, smoking, diabetes duration, body mass index, systolic blood pressure, glycated hemoglobin, total cholesterol, estimated glomerular filtration rate), serum levels of phosphate and magnesium were still related to status of DPN in logistic regression (P < 0.05).

Conclusion: Lower serum phosphate and magnesium significantly correlated with parameters of nerve conduction in T2DM patients. Serum phosphate and magnesium might underlie the pathophysiologic features of DPN.
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http://dx.doi.org/10.1016/j.diabres.2018.09.015DOI Listing
December 2018

A New Surgical Method of Correcting Abnormal Cartilage in Mild or Moderate Cryptotia.

Ann Plast Surg 2018 12;81(6):662-668

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin Medical University, Nangang District, Harbin, Heilongjiang Province, China.

Objective: This study aimed to introduce a new technique for the correction of mild or moderate types of cryptotia.

Methods: During January 2010 to March 2015, patients with cryptotia deformities came to our hospital and were enrolled in this study. A new surgical method of correction of abnormal cartilage by combining irregular incisions in auricle malformation and directional transplantation of auricular cartilage was designed. The irregular Z shaped flap was designed to correct the skin defects. In the ipsilateral cavum conchae, the auricular cartilage strut was harvested, followed with irregular cartilage incisions and cartilage transplantation.

Results: After correction, the corrected auricles in 3 cases showed natural contour with deep auriculotemporal sulcus and no conspicuous scars. The antihelix folding and backward rotation deformities of the auricular cartilage were corrected, and the morphology of superior and inferior crus of the antihelix was not destroyed. One case appeared necrotic. During 1 year of follow-up, there was no incidence of recurrence or revision.

Conclusions: The combined techniques for correction of abnormal cartilage are simple and easy to operate, and the reconstructed auricles show natural contour without conspicuous scars. The new technique is suitable for correction of mild and moderate cryptotia.
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http://dx.doi.org/10.1097/SAP.0000000000001591DOI Listing
December 2018

Prevalence and patterns of drug resistance among pulmonary tuberculosis patients in Hangzhou, China.

Antimicrob Resist Infect Control 2018 2;7:61. Epub 2018 May 2.

1Hangzhou Center for Disease Control and Prevention, Mingshi Road, Hangzhou City, 310021 Zhejiang Province China.

Background: To evaluate prevalence and patterns of drug resistance among pulmonary tuberculosis (TB) patients in Hangzhou City, China.

Methods: Sputum samples of smear positive TB patients enrolled in 2011 and 2015 were collected and tested for drug susceptibility, and demographic and medical record data were extracted from the electronic database of China Information System for Disease Control and Prevention. Chi-square test was used to compare drug resistance prevalence between new and treated patients and between male and female patients, and Chi-square test for trend was used to compare the prevalence over calendar years 2011 and 2015.

Results: Of 1326 patients enrolled in 2015, 22.3% had resistance to any first-line anti-TB drugs and 8.0% had multi-drug resistance (MDR); drug resistance rates among previously treated cases were significantly higher than among new cases. Significant declines of resistance to isoniazid, rifampin, ethambutol and streptomycin, and MDR from 2011 to 2015 were observed among previously treated patients, while a significant decline of resistance to rifampin was observed among new cases.

Conclusions: While the prevalence of acquired drug resistance decreased due to due to implementation of DOTS-Plus program, the prevalence of primary drug resistance due to transmission remained high. Greater efforts should be made to screen drug resistance for case finding and to reduce transmission through improving the treatment and management of drug-resistant patients.
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http://dx.doi.org/10.1186/s13756-018-0348-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5930636PMC
July 2019

The Individualized Facelift Technique in Improving Facial Asymmetry for Asian Patients.

Ophthalmic Plast Reconstr Surg 2018 Nov/Dec;34(6):516-521

Division of Plastic Surgery, The Second Hospital of Harbin Medical University, Harbin, Heilongjiang, People's Republic of China.

Purpose: Among multiple influential factors affecting facial symmetry, the role of soft tissue is often overlooked. Skin and skeletal differences between Asian and Caucasian people also require the adaptation of current techniques for Asian patients. This article aimed to explore the ability of individual facelift techniques to improve facial symmetry and reset youthful eye in Asian people, while a new method, called the grid method, was tried to evaluate the improvement in facial symmetry.

Methods: The authors conducted a review of 58 consecutive facelifts, which were all performed by a single surgeon between April 2009 and December 2016 following institutional review board approval. Among them, 21 patients underwent lower eyelid blepharoplasty. The original frontal photograph of each patient was evaluated by the grid method. Five independent plastic surgeons reviewed the facial asymmetry of the images before and after the operations using a visual analog scale to analyze the facial asymmetry of the patients.

Results: In the preoperative group evaluated by the grid, the mean facial asymmetry score was 4.11, while in the postoperative group, the mean score was 1.07, which was significantly lower than the mean score before the operation (p < 0.001). The change in mean scores illustrated that the technique was effective in improving facial symmetry in Asian people. A total of 8 patients experienced hematomas and recovered well without obvious sequelae.

Conclusions: The individual facelift technique was effective for improving facial symmetry and reshaping youthful eye in Asian people.
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http://dx.doi.org/10.1097/IOP.0000000000001072DOI Listing
May 2019

Development and in vivo validation of tissue-engineered, small-diameter vascular grafts from decellularized aortae of fetal pigs and canine vascular endothelial cells.

J Cardiothorac Surg 2017 Nov 25;12(1):101. Epub 2017 Nov 25.

Department of Plastic Surgery, The Second Affiliated Hospital of Harbin Medical University, 246 Xuefu Road, Nangang District, Harbin, Heilongjiang, 150086, China.

Background: Tissue engineering has emerged as a promising alternative for small-diameter vascular grafts. The aim of this study was to determine the feasibility of using decellularized aortae of fetal pigs (DAFPs) to construct tissue-engineered, small-diameter vascular grafts and to test the performance and application of DAFPs as vascular tissue-engineered scaffolds in the canine arterial system.

Methods: DAFPs were prepared by continuous enzymatic digestion. Canine vascular endothelial cells (ECs) were seeded onto DAFPs in vitro and then the vascular grafts were cultured in a custom-designed vascular bioreactor system for 7 days of dynamic culture following 3 days of static culture. The grafts were then transplanted into the common carotid artery of the same seven dogs from which ECs had been derived (two grafts were prepared for each dog with one as a backup; therefore, a total of 14 tissue-engineered blood vessels were prepared). At 1, 3, and 6 months post-transplantation, ultrasonography and contrast-enhanced computed tomography (CT) were used to check the patency of the grafts. Additionally, vascular grafts were sampled for histological and electron microscopic examination.

Results: Tissue-engineered, small-diameter vascular grafts can be successfully constructed using DAFPs and canine vascular ECs. Ultrasonographic and CT test results confirmed that implanted vascular grafts displayed good patency with no obvious thrombi. Six months after implantation, the grafts had been remodeled and exhibited a similar structure to normal arteries. Immunohistochemical staining showed that cells had evenly infiltrated the tunica media and were identified as muscular fibroblasts. Scanning electron microscopy showed that the graft possessed a complete cell layer, and the internal cells of the graft were confirmed to be ECs by transmission electron microscopy.

Conclusions: Tissue-engineered, small-diameter vascular grafts constructed using DAFPs and canine vascular ECs can be successfully transplanted to replace the canine common carotid artery. This investigation potentially paves the way for solving a problem of considerable clinical need, i.e., the requirement for small-diameter vascular grafts.
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http://dx.doi.org/10.1186/s13019-017-0661-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5702065PMC
November 2017
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