Publications by authors named "Qing-Lin Li"

50 Publications

Amygdala dynorphin/κ opioid receptor system modulates depressive-like behavior in mice following chronic social defeat stress.

Acta Pharmacol Sin 2021 May 25. Epub 2021 May 25.

Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

Major depression disorder is a severe and recurrent neuropsychological disorder characterized by lowered mood and social activity and cognitive impairment. Owing to unclear molecular mechanisms of depression, limited interventions are available in clinic. In this study we investigated the role of dynorphin/κ opioid receptor system in the development of depression. Mice were subjected to chronic social defeat stress for 14 days. Chronic social defeat stress induced significant social avoidance in mice characterized by decreased time duration in the interaction zone and increased time duration in the corner zone. Pre-administration of a κ opioid receptor antagonist norBNI (10 mg/kg, i.p.) could prevent the development of social avoidance induced by chronic social defeat stress. Social avoidance was not observed in κ opioid receptor knockout mice subjected to chronic social defeat stress. We further revealed that social defeat stress activated c-fos and ERK signaling in the amygdala without affecting the NAc, hippocampus and hypothalamus, and ERK activation was blocked by systemic injection of norBNI. Finally, the expression of dynorphin A, the endogenous ligand of κ opioid receptor, was significantly increased in the amygdala following social defeat stress; microinjection of norBNI into the amygdala prevented the development of depressive-like behaviors caused by social defeat stress. The present study demonstrates that upregulated dynorphin/κ opioid receptor system in the amygdala leads to the emergence of depression following chronic social defeat stress, and sheds light on κ opioid receptor antagonists as potential therapeutic agents for the prevention and treatment of depression following chronic stress.
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http://dx.doi.org/10.1038/s41401-021-00677-6DOI Listing
May 2021

[ acupuncture combined with computer-assisted cognitive training for post stroke cognitive impairment: a randomized controlled trial].

Zhongguo Zhen Jiu 2021 Mar;41(3):247-51

Rehabilitation Medicine Center of Hubei Hospital of Integrated Chinese and Western Medicine, Wuhan 430015, China.

Objective: To explore the effect of acupuncture combined with computer-assisted cognitive training on the recovery of cognitive function and activities of daily living in patients with post stroke cognitive impairment.

Methods: A total of 98 patients with post stroke cognitive impairment were randomized into an observation group (50 cases, 6 cases dropped off) and a control group (48 cases, 5 cases dropped off). Both groups were treated with conventional treatment, such as computer-assisted cognitive training. On the basis of the conventional treatment, acupuncture at Taixi (KI 3), Sanyinjiao (SP 6), Shuigou (GV 26), Baihui (GV 20), ect. was given in the observation group. In the control group, acupuncture at acupoints of limbs was given. The treatment was given once a day, 5 times a week for 8 weeks. Before and after treatment, the scores of Montreal cognitive assessment (MoCA) scale, modified Barthel index (MBI) and stroke syndrome of TCM scale were used to evaluate the cognitive function, activities of daily living and syndrome of TCM in the two groups. The latency and amplitude of P300 were detected by electromyographs and evoked response instrument. And the clinical efficacy was evaluated in the two groups.

Results: Compared before treatment, the MoCA and MBI scores were increased (<0.01), and the scores of stroke syndrome of TCM scale were decreased (<0.01) after treatment in the two groups. After treatment,the MoCA and MBI scores in the observation group were higher than the control group (<0.01, <0.05), and the score of stroke syndrome of TCM scale was lower than the control group (<0.05). Compared before treatment, the latency of P300 was shortened and amplitude was prolonged after treatment in the two groups (<0.01). After treatment, in the observation group, the latency of P300 was shorter, and amplitude was longer than the control group (<0.01). The effective rate was 86.4% (38/44) in the observation group, which was higher than 67.4% (29/43) in the control group (<0.01).

Conclusion: acupuncture combined with computer-assisted cognitive training could improve the cognitive function of patients with post stroke cognitive impairment.
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http://dx.doi.org/10.13703/j.0255-2930.20200311-k0001DOI Listing
March 2021

Comparison of survival between adolescent and young adult older patients with hepatocellular carcinoma.

World J Gastrointest Oncol 2020 Dec;12(12):1394-1406

Department of Geriatric Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.

Background: Due to the special clinical features and biologic characteristics of adolescent and young adult (AYA) cancers, AYA cancers are different from cancers in children and elderly individuals. However, there are few reports on AYA hepatocellular carcinoma (HCC).

Aim: To investigate the overall survival (OS) of AYA (15-39 years) and elderly (40-74 years) patients with HCC.

Methods: The data of all the HCC cases were extracted from the Surveillance, Epidemiology, and End Results database from 2004 to 2015 and were then divided into two groups based on age: AYA group (15-39 years) and older group (40-74 years). Kaplan-Meier curves and log-rank tests were used to compare the OS of the two groups. Propensity score matching (PSM) was employed to analyze the OS difference between the two groups. The Cox proportional hazards regression model was used to perform multivariate analysis to explore the risk factors for OS of HCC patients.

Results: Compared to elderly cancer patients, AYA patients with HCC had a worse Surveillance, Epidemiology, and End Results stage, including the distant stage (22.1% 15.4%, < 0.001), and a more advanced American Joint Committee on Cancer (AJCC) stage, including AJCC III and IV (49.2% 38.3%, < 0.001), and were more likely to receive surgery (64.5% 47.5%, < 0.001). Before PSM, the AYA group had a longer survival in months (median: 20.00, interquartile range [IQR]: 5.00-62.50) than the older group (median: 15.00, IQR: 4.00-40.00) ( < 0.001). After PSM, the AYA group still had a longer survival in months (median: 21.00, IQR: 5.00-64.50) than the older group (median: 18.00, IQR: 6.00-53.00) ( < 0.001). The Cox proportional hazards regression model showed that advanced age (hazard ratio [HR] = 1.405, 95%CI: 1.218-1.621, < 0.001) was a risk factor for OS of HCC patients. In the subgroup analysis, the Cox proportional hazards regression model showed that in AJCC I/II HCC patients, advanced age (HR = 1.749, 95%CI: 1.352-2.263, < 0.001) was a risk factor for OS, while it was not a risk factor in AJCC III/IV HCC patients (HR = 1.186, 95%CI: 0.997-1.410, = 0.054) before PSM. After PSM, advanced age (HR = 1.891, 95%CI: 1.356-2.637, < 0.001) was still a risk factor for OS in AJCC I/II HCC patients, but was not a risk factor for OS in AJCC III/IV HCC patients (HR = 1.192, 95%CI: 0.934-1.521, = 0.157) after PSM.

Conclusion: AYA patients with HCC have different clinical characteristics from older adults. In different AJCC stages, the two groups of patients have different OS: In AJCC I/II HCC patients, advanced age is a risk factor for OS, but it is not a risk factor for OS in the AJCC III/IV HCC patient group.
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http://dx.doi.org/10.4251/wjgo.v12.i12.1394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7739151PMC
December 2020

Ferrostatin-1 protects HT-22 cells from oxidative toxicity.

Neural Regen Res 2020 Mar;15(3):528-536

Xin'an Key Laboratory of Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, Anhui Province, China.

Ferroptosis is a type of programmed cell death dependent on iron. It is different from other forms of cell death such as apoptosis, classic necrosis and autophagy. Ferroptosis is involved in many neurodegenerative diseases. The role of ferroptosis in glutamate-induced neuronal toxicity is not fully understood. To test its toxicity, glutamate (1.25-20 mM) was applied to HT-22 cells for 12 to 48 hours. The optimal experimental conditions occurred at 12 hours after incubation with 5 mM glutamate. Cells were cultured with 3-12 μM ferrostatin-1, an inhibitor of ferroptosis, for 12 hours before exposure to glutamate. The cell viability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Autophagy was determined by monodansylcadaverine staining and apoptosis by caspase 3 activity. Damage to cell structures was observed under light and by transmission electron microscopy. The release of lactate dehydrogenase was detected by the commercial kit. Reactive oxygen species were measured by flow cytometry. Glutathione peroxidase activity, superoxide dismutase activity and malondialdehyde level were detected by the appropriate commercial kit. Prostaglandin peroxidase synthase 2 and glutathione peroxidase 4 gene expression was detected by real-time quantitative polymerase chain reaction. Glutathione peroxidase 4 and nuclear factor erythroid-derived-like 2 protein expression was detected by western blot analysis. Results showed that ferrostatin-1 can significantly counter the effects of glutamate on HT-22 cells, improving the survival rate, reducing the release of lactate dehydrogenase and reducing the damage to mitochondrial ultrastructure. However, it did not affect the caspase-3 expression and monodansylcadaverine-positive staining in glutamate-injured HT-22 cells. Ferrostatin-1 reduced the levels of reactive oxygen species and malondialdehyde and enhanced superoxide dismutase activity. It decreased gene expression of prostaglandin peroxidase synthase 2 and increased gene expression of glutathione peroxidase 4 and protein expressions of glutathione peroxidase 4 and nuclear factor (erythroid-derived)-like 2 in glutamate-injured HT-22 cells. Treatment of cultured cells with the apoptosis inhibitor Z-Val-Ala-Asp (OMe)-fluoromethyl ketone (2-8 μM), autophagy inhibitor 3-methyladenine (100-400 μM) or necrosis inhibitor necrostatin-1 (10-40 μM) had no effect on glutamate induced cell damage. However, the iron chelator deferoxamine mesylate salt inhibited glutamate induced cell death. Thus, the results suggested that ferroptosis is caused by glutamate-induced toxicity and that ferrostatin-1 protects HT-22 cells from glutamate-induced oxidative toxicity by inhibiting the oxidative stress.
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http://dx.doi.org/10.4103/1673-5374.266060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921338PMC
March 2020

Preventive Effect and Mechanism of Ethyl Acetate Extract of Sceptridium ternatum in Monocrotaline-Induced Pulmonary Arterial Hypertension.

Chin J Integr Med 2020 Mar 28;26(3):205-211. Epub 2018 Dec 28.

Department of Pharmacy, Zhejiang Cancer Hospital, Hangzhou, 310022, China.

Objective: To observe the effect and molecular mechanism of ethyl acetate extract of Sceptridium ternatum (STE) on the monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH).

Methods: The main chemical components of Sceptridium ternatum were determined, and the effects in PAH rats were observed. A total of 140 Sprague Dawley rats were randomly and equally divided into the normal group, the model group, the Bosentan group, and the STE groups (2.5, 5, 10 g/kg) by the random number table method. The characteristic indicators of PAH were measured, and immunohistochemistry was used to observe the lung tissue of rats. Morphological changes of the lung tissue were observed under the light microscope.

Results: Compared with the normal group, rats in the model group showed a significant increase in right ventricular free wall thickness (RVFWT), mean pulmonary arterial pressure (mPAP), mean right ventricular pressure (mRVP), max right ventricular pressure (max RVP), weight of right ventricle (RV), and lung index (LI), while a significant decrease in pulmonary artery acceleration time (PAAT, P<0.01). Compared with the model group, rats treated with STE had a significant decrease of RVFWT, mPAP, mRVP, max RVP, and RV, while a significant increase of PAAT (P<0.01). After injection of MCT, nuclear factor- κB (NF- κB) p65 and α -smooth muscle actin (α -SMA) expression levels were up-regulated, and on the contrary, the treatment groups showed a significant down-regulation without dose-dependent trend.

Conclusions: STE can relieve the PAH in rats. STE may relieve pulmonary vascular disease and pulmonary injury by down-regulating the expression of NF- κB p65 and α -SMA.
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http://dx.doi.org/10.1007/s11655-018-2573-6DOI Listing
March 2020

[Mechanism of gambogenic acid in resisting angiogenesis of lung cancer in vitro].

Zhongguo Zhong Yao Za Zhi 2018 Nov;43(21):4311-4316

Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, China.

The aim of this paper was to observe the effect of gambogenic acid on angiogenesis of lung cancer and its preliminary mechanism. After culturing lung adenocarcinoma A549 cells, the conditioned medium was treated with gambogenic acid and then used to culture human umbilical vein endothelial cells (HUVECs) to establish the indirect contact cell co-culture system. A two-dimensional culture model of HUVEC was established with matrigel to observe the effect of gambogenic acid on angiogenesis. DAPI staining was used to observe the morphological changes in HUVEC cells after treatment with gambogenic acid under the fluorescence microscope. Annexin V-FITC/PI staining and flow cytometry analysis were used to determine gambogenic acid's effect on HUVEC cell apoptosis rate. The protein expressions of PI3K, p-PI3K, Akt, p-Akt were measured by Western blot. PTEN-siRNA was transfected into cells, and RT-PCR was used to detect the expression levels of PI3K and Akt genes. Gambogenic acid can significantly inhibit angiogenesis, and its inhibitory effect was dose-dependent. DAPI staining showed apoptotic morphological features of HUVEC cells under fluorescence microscope. Annexin V-FITC/PI staining showed that gambogenic acid induced apoptosis in HUVECs. The results of Western blot showed that the expressions of p-PI3K and p-Akt protein were down-regulated with gambogenic acid, while the expressions of PI3K and Akt protein was insignificant. The results of RT-PCR indicated that the expressions of PI3K and Akt protein were up-regulated by PTEN siRNA. Gambogenic acid can inhibit angiogenesis in lung cancer in vitro, and the mechanism of inhibiting angiogenesis may be related to the PI3K/Akt signaling pathway.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20180601.001DOI Listing
November 2018

[Effects of Huoxue Dingxuan Capsule Glare on Vertebral Artery Blood Flow,Plasma PAI and t-PA in CSA Rat Models.]

Sichuan Da Xue Xue Bao Yi Xue Ban 2016 Nov;47(6):862-865

Clinical College of Traditional Chinese Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China.

Objectives: To determine the effects of Huoxue Dingxuan Capsule on vertebral artery blood flow,plasma plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) in rats with cervical spondylosis of vertebral artery type (CSA).

Methods: Ninety healthy male Wistar rats were equally and randomly divided into control,model and treatment groups.The rats in the model and treatment groups were subject to composite modeling manufacturing CSA.The treatment group was given six-week interventions with Huoxue Dingxuan capsule 4 weeks after the modeling.Vertebral artery blood flow,plasma PAI,and t-PA contents were detected before modeling,prior to the interventions,and post interventions.

Results: Before the interventions,the rats in the model and treatment groups had significantly lower blood flow of vertebral artery than the controls (<0.05).The model rats also had increased serum PAI and t-PA contents (<0.01).After the interventions,significantly higher vertebral blood flow was found in the treatment group compared with the controls (<0.05).After the interventions,increased serum PAI and t-PA contents were observed in the rats in the model group (<0.01);whereas,decreased serum PAI and t-PA contents were observed in the rats in the treatment group (<0.01).The treatment group had lower levels of serum PAI and t-PA contents than the model group (<0.01).

Conclusions: Huoxue Dingxuan Capsule glare can improve the blood flow of vertebral artery and reduce serum PAI and t-PA contents.
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November 2016

Anfibatide protects against rat cerebral ischemia/reperfusion injury via TLR4/JNK/caspase-3 pathway.

Eur J Pharmacol 2017 Jul 5;807:127-137. Epub 2017 Apr 5.

Department of Pharmacology, Anhui Medical University, Hefei, Anhui 230032, China. Electronic address:

Anfibatide (ANF) is a GPIb antagonist derived from the protein complex agglucetin. Previous studies have showed that it has protective effect on cerebral ischemia/reperfusion injury, the mechanism of which is still unclear, however. The present study was designed to investigate the protective effect of ANF on cerebral I/R injury in rats and the possible mechanisms. Focal cerebral ischemia was induced by 90min of transient middle cerebral artery occlusion (MCAO). ANF (1, 2, 4μg/kg) was achieved by intravenous injection after 120min of MCAO followed by 1h, 24h ,48h and 72h reperfusion. Neurological deficit, infarct volume, histopathology, neuronal apoptosis, NeuN and the expression of TLR4, total and phosphorylated c-Jun NH-terminal kinase (JNK/p-JNK), Bcl-2, Bax, caspase-3, NF-κB protein in rat brain, the levels of IL-1β, IL-6 and TNF-α in serum were evaluated 72h after reperfusion. ANF could significantly decrease neurological score, reduce the infarct volumes, ameliorate the histopathological alteration, attenuate the neuronal apoptosis and increase the fluorescence density of NeuN in the rat brain. Furthermore, ANF could obviously decrease the expression of TLR4, p-JNK, caspase-3, NF-κB , relative ratio of Bax/Bcl-2 in brain and the levels of IL-1β, IL-6 and TNF-α in serum. The results indicate that ANF has protective effect against cerebral I/R injury in rats and the underlying mechanism may be associated with the suppression of apoptosis through inhibiting TLR4/JNK/caspase-3 signaling pathway.
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http://dx.doi.org/10.1016/j.ejphar.2017.04.002DOI Listing
July 2017

Two Unusual Nanocage-Based Ln-MOFs with Triazole Sites: Highly Fluorescent Sensing for Fe and Cr O , and Selective CO Capture.

Chempluschem 2016 Dec 30;81(12):1299-1304. Epub 2016 Sep 30.

School of Chemistry, Monash University, Melbourne, VIC, 3800, Australia.

Luminescent metal-organic frameworks (LMOFs) containing fluorescent probes for the detection of pollutants such as organic solvents and heavy metals are becoming increasingly important, with lanthanide-MOF (Ln-MOF) materials receiving greater attention owing to the possibility of achieving fine-tuned luminescent properties. Herein, two unusual isostructural nanocage-based three-dimensional Ln-MOFs, 1-Ln (Ln=Tb, Eu), are constructed, using a new diisophthalate ligand with active Lewis basic triazole sites. Selective gas adsorption, especially the removal of CO from CH , a primary component of natural gas and biogas, is desirable in terms of both economic and environmental considerations. 1-Eu is found to exhibit highly efficient luminescent sensing for Fe cations and Cr O anions, as well as selective CO capture over CH .
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http://dx.doi.org/10.1002/cplu.201600289DOI Listing
December 2016

Development of neural basis for chinese orthographic neighborhood size effect.

Hum Brain Mapp 2016 Feb 19;37(2):632-47. Epub 2015 Nov 19.

Key Laboratory of Behavioral Science, Institute of Psychology, Chinese Academy of Sciences, Beijing, 100101, China.

The brain activity of orthographic neighborhood size (N size) effect in Chinese character naming has been studied in adults, meanwhile behavioral studies have revealed a developmental trend of Chinese N-size effect in developing readers. However, it is unclear whether and how the neural mechanism of N-size effect changes in Chinese children along with development. Here we address this issue using functional magnetic resonance imaging. Forty-four students from the 3(rd) , 5(th) , and 7(th) grades were scanned during silent naming of Chinese characters. After scanning, all participants took part in an overt naming test outside the scanner, and results of the naming task showed that the 3(rd) graders named characters from large neighborhoods faster than those from small neighborhoods, revealing a facilitatory N-size effect; the 5(th) graders showed null N-size effect while the 7(th) graders showed an inhibitory N-size effect. Neuroimaging results revealed that only the 3(rd) graders exhibited a significant N-size effect in the left middle occipital activity, with greater activation for large N-size characters. Results of 5(th) and 7(th) graders showed significant N-size effects in the left middle frontal gyrus, in which 5(th) graders induced greater activation in large N-size condition than in small N-size condition, while 7(th) graders exhibited an opposite effect which was similar to the adult pattern reported in a previous study. The current findings suggested the transition from broadly tuned to finely tuned orthographic representation with reading development, and the inhibition from neighbors' phonology for higher graders. Hum Brain Mapp 37:632-647, 2016. © 2015 Wiley Periodicals, Inc.
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http://dx.doi.org/10.1002/hbm.23055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6867302PMC
February 2016

Investigation on Molecular Mechanism of Fibroblast Regulation and the Treatment of Recurrent Oral Ulcer by Shuizhongcao Granule-Containing Serum.

Evid Based Complement Alternat Med 2015 18;2015:324091. Epub 2015 Oct 18.

The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, China.

The purpose is to study the intervention, proliferation, and differentiation on fibroblast by Shuizhongcao Granule during the treatment of ROU and investigate the action mechanism in inflammatory microenvironment. Proliferation of rat fibroblasts was detected using CCK8. Western blot was used to detect the effect of drug-containing serum on the expression of protein associated with NF-κB and ERK pathway in rat fibroblasts. Expression of IL-10 and IL-12 was detected by PCR. Shuizhongcao Granule group successfully inhibited proliferation of rat fibroblast. Western blot results revealed that p65 and IKKB were significantly less expressed in Chinese medicine group, while pIκBα and pIKKαβ expression were significantly increased. We have also found that in this group the expression of pAKT was evidently suppressed and expression of pERK significantly decreased. PCR results showed significantly decreased expression levels of IL-10 and 1IL-12b in Chinese medicine group, while the expression of IL-12a was increased. Our results suggest that Shuizhongcao Granule can suppress the proliferation of fibroblast and inhibit the activation of NF-κB and thus suppress inflammatory reactions, possibly involving the inhibited expression of phosphorylated AKT, rather than the canonical pathway. Furthermore, it can inhibit ERK pathway and reduce IL-10 and IL-12b gene expression while enhancing IL-12a expression.
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http://dx.doi.org/10.1155/2015/324091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4628681PMC
November 2015

Predictors and impact of cytotoxic second-line chemotherapy for stage IIIa-IV nonsmall lung cancer patients in China: A retrospective institution analysis of 132 patients.

J Cancer Res Ther 2015 Aug;11 Suppl 1:C84-8

Department of Pharmacy, Zhejiang Cancer Hospital, Hangzhou 310022, Zhejiang Province, People's Republic of China.

Objective: To investigate the predictors and impact of cytotoxic second-line chemotherapy for stage IIIa-IV nonsmall cell lung cancer (NSCLC) patients in China.

Methods: Medical records of 132 patients who underwent chemotherapy from January 2008 to December 2010 in our hospital were retrospectively reviewed. The response of first-line gemcitabine (GEM) and platinum doublets chemotherapy was evaluated, and the overall survival (OS) of all patients was followed. Further, risk factors of receipt cytotoxic second-line chemotherapy or not were identified and evaluated by univariate analyses.

Results: Sixty-six cases have undergone cytotoxic second-line chemotherapy for lung cancer. The OS between patients received first-line GEM plus platinum doublets chemotherapy and patients without cytotoxic second chemotherapy had no statistical difference (P = 0.73). Smoking or not might be a meaningful predictor for cytotoxic second-line therapy among these patients in this investigation (P < 0.05). Other factors, such as age (≥ 65 or < 65), gender, alcohol use, hypertension, diabetes mellitus, histology type, number of cycles of first-line chemotherapy, and response of first-line chemotherapy had no statistical difference between patients received first-line GEM plus platinum doublets chemotherapy and patients received cytotoxic second chemotherapy (P > 0.05). In the sub-analysis, we found that the OS between patients received first-line GEM plus platinum doublets chemotherapy and patients without cytotoxic second chemotherapy had statistical difference in the population whose OS < 540 days (P = 0.019). Moreover, in these patients, the blood type was found to be a selected factor in receiving cytotoxic second-line chemotherapy or not (P < 0.05). Whereas other factors were not shown their selected effect (P > 0.05).

Conclusion: This study demonstrated that though the essentiality of cytotoxic second-line chemotherapy for stage IIIa-IV EGFR mutation indefinite NSCLC is unclear, patients who are blood type AB with poor prognosis and short OS might be a dominant population for cytotoxic second-line chemotherapy.
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http://dx.doi.org/10.4103/0973-1482.163849DOI Listing
August 2015

Enhanced growth suppression of TERT-positive tumor cells by oncolytic adenovirus armed with CCL20 and CD40L.

Int Immunopharmacol 2015 Sep 24;28(1):487-93. Epub 2015 Jul 24.

Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, China-Japan Union Hospital, Jilin University, Changchun 130062, PR China. Electronic address:

Conditionally replicating adenoviruses (CRAds) selectively replicate in cancer cells and induce cell lysis, which represents a potential platform for cancer immunotherapy. The chemokine CCL20 exerts antitumor activity via chemoattraction of immature dendritic cells (DCs) and lymphocytes. However, the activation and maturation status of DCs is a limiting factor in the DCs -based immunity response. CD40L induces the phenotypic maturation of DCs, mediates DCs cytokine secretion, and increases the expression of FasL, which mediates apoptosis. We constructed a CCL20/CD40L co-expression CRAds (Ad-CCL20-CD40L) based on the AdEasy system. Ad-CCL20-CD40L was constructed from three plasmids, pGTE-CD40L, pShuttle-CMV-CCL20 and AdEasy-1, and was homologously recombined and propagated in the Escherichia coli strain BJ5183 and the packaging cell line HEK-293, respectively. Ad-CCL20-CD40L selectively replicates in TERT-positive tumor cells because the pGTE-CD40L plasmid contains the telomerase reverse transcriptase promoter (TERTp). Our results showed that Ad-CCL20-CD40L induced oncolytic effects and tumor-specific cytotoxicity of cytotoxic T lymphocytes (CTLs) in vitro. This study suggests that Ad-CCL20-CD40L can induce the antitumor immune response and that this platform can be modified to generate novel CRAds with other transgenes.
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http://dx.doi.org/10.1016/j.intimp.2015.07.005DOI Listing
September 2015

[Study on the polarized reflectance-hyperspectral information fusion technology of tomato leaves nutrient diagnoses].

Guang Pu Xue Yu Guang Pu Fen Xi 2014 Sep;34(9):2500-5

With 25%, 50%, 75%, 100% and 150%, five levels of, nitrogen (N), phosphorus (P) and potassium (K) nutrition stress samples cultivated in Venlo type greenhouse soilless cultivation mode as the research object, polarized reflectance spectra and hyperspectral images of different nutrient deficiency greenhouse tomato leaves were acquired by using polarized reflectance spectroscopy system developed by our own research group and hyperspectral imaging system respectively. The relationship between a certain number of changes in the bump and texture of non-smooth surface of the nutrient stress leaf and the level of polarization reflected radiation was clarified by scanning electron microscopy (SEM). On the one hand, the polarization spectrum was converted into the degree of polarization through Stokes equation, and the four polarization characteristics between the polarization spectroscopy and reference measurement values of N, P and K respectively were extracted. On the other hand, the four characteristic wavelengths of N, P, K hyperspectral image data were determined respectively through the principal component analysis, followed by eight hyperspectral texture features extracted corresponding to the four characteristic wavelengths through correlation analysis. Polarization characteristics and hyperspectral texture features combined with each characteristics of N, P, K were extracted. These 12 characteristic variables were normalized by maximum-minimum value method. N, P, K nutrient levels quantitative diagnostic models were established by SVR. Results of models are as follows: the correlation coefficient of nitrogen r = 0.961 8, root mean square error RMSE= 0.451; correlation coefficient of phosphorus r = 0.916 3, root mean square error RMSE = 0.620; correlation coefficient of potassium r = 0.940 6, root mean square error RMSE = 0.494. The results show that high precision tomato leaves nutrition prediction model could be built by using polarized reflectance spectroscopy combined with high spectral information fusion technology and achieve good diagnoses effect. It has a great significance for the improvement of model accuracy and the development of special instruments. The research provides a new idea for the rapid detection of tomato nutrient content.
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September 2014

Two isoreticular metal-organic frameworks with CdSO4-like topology: selective gas sorption and drug delivery.

Dalton Trans 2014 Dec;43(46):17265-73

School of Pharmacy, Guangdong Medical College, Dongguan, 523808, P. R. China.

Two isoreticular metal-organic frameworks with chemical formulae [Cu(L)(4,4'-bipy)(H2O)]n 1.5nCH3CN (1) and [Cu(L)(4,4'-bipy)(H2O)]n·4nH2O (2) (H2L = diphenylmethane-4,4'-dicarboxylic acid) were synthesized and structurally characterized. They show the CdSO4 (6(5) 8) net and have an obvious 1D channel that is spread along the crystallographic c axis. More importantly, 1 shows high selectivity for H2 over N2 and CO2 at low pressure, which could be confirmed via computational calculations using the Connolly algorithm to reveal the size and shape of accessible voids. The incorporation of the drug 5-fluorouracil (5-FU) into the desolvated 1 was around 27.5 wt% per gram of the dehydrated 1. 5-FU is released in a highly controlled and progressive fashion with 61% of the drug released after 95 hours. In addition, we have applied molecular docking calculations to investigate the preferred conformation of 5-FU molecules upon binding to MOF 1. These calculations provide a structural basis to explain the 5-FU release from MOF 1.
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http://dx.doi.org/10.1039/c4dt01890gDOI Listing
December 2014

[Apoptosis of melanoma B16 cells induced by gambogenic acid].

Zhong Yao Cai 2014 Mar;37(3):469-73

Objective: To study the inhibitory effect of Gambogenic acid (GNA) on melanoma B16 cells proliferation, and to explore the role of cell apoptosis.

Methods: The inhibitory effect of GNA on the proliferation of B16 cells was measured by methyl thiazolyl tetrazolium (MTT) assay; Alternation of B16 cells ultrastructure was detected by AO/EB staining under fluorescent microscope; Flow cytometry was used to detect intracellular reactive oxygen species (ROS) in B16 cells generated by GNA treatment Western blotting was used to investigate the expression of intracellular Caspase-3 proteins changes.

Results: MTT results showed that the GNA within a certain time and a certain concentration significantly suppressed the proliferation of B16 cells and morphological changes were observed by fluorescence microscope on B16 cells after GNA treatment. AO/EB staining showed that the major cell density decreased. GNA treated cells showed obvious apoptotic status. After the cells treated with GNA, in a short period of time, intracellular ROS levels increased dramatically compared with the control group (P < 0.01), and the mitochondrial membrane had a low potential consistently. Western blotting results showed that changes of intracellular proteins expression in the release of Caspase-3 proteins expression levels were increased after GNA treatment.

Conclusion: GNA can inhibit malignant melanoma B16 cells growth and proliferation and induce apoptosis within a certain time and at a certain concentration.
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March 2014

[Study of gambogenic acid-induced apoptosis of melanoma B16 cells through PI3K/Akt/mTOR signaling pathways].

Zhongguo Zhong Yao Za Zhi 2014 May;39(9):1666-9

Objective: To discuss the mechanism of gambogenic acid (GNA) in inducing the apoptosis of melanoma B16 cells.

Method: The inhibitory effect of GNA on the proliferation of B16 cells was measured by the methyl thiazolyl tetrazolium (MTT) assay. The effect of GNA on B16 cells was detected by the Hoechst 33258 staining. The transmission electron microscopy was used to observe the ultra-structure changes of B16 cells. The changes in PI3K, p-PI3K, Akt, p-Akt, p-mTOR, PTEN proteins were detected by the Western blotting to discuss the molecular mechanism of GNA in inducing the apoptosis of B16 cells.

Result: GNA showed a significant inhibitory effect in the growth and proliferation of melanoma B16 cells. The cell viability remarkably decreased with the increase of GNA concentration and the extension of the action time. The results of the Hoechst 33258 staining showed that cells processed with GNA demonstrated apparent apoptotic characteristics. Under the transmission electron microscope, B16 cells, after being treated with GNA, showed obvious morphological changes of apoptosis. The Western blot showed a time-dependent reduction in the p-PI3K and p-Akt protein expressions, with no change in p-PI3K and p-Akt protein expression quantities. The p-mTOR protein expression decreased with the extension of time, where as the PTEN protein expression showed a time-dependent increase.

Conclusion: GNA could inhibit the proliferation of melanoma B16 cells and induce their apoptosis within certain time and concentration ranges. Its mechanism in inducing the cell apoptosis may be related to PI3K/Akt/mTOR signaling pathways.
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May 2014

[Gambogenic acid induces mitochondria-dependent apoptosis in human gastric carcinoma cell line].

Zhong Yao Cai 2014 Jan;37(1):95-9

Objective: To study the effects of Gambogenic acid (GNA) on the growth of human gastric carcinoma cell line MGC-803 and its underlying mechanisms.

Methods: MTT assay was used to measure the cell viability. Apoptosis, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) were detected using flow cytometry method. Among them, Annexin V-FITC/PI double staining was employed in the analysis of apoptosis, Rh123 in analyzing MMP and H2DCFDA in analyzing ROS formation. P53 expression was confirmed by Western blot.

Results: 4.0 micromol/L GNA inhibited MGC-803 cells growth in a time dependent manner from 24 to 48 h. At the concentration range from 1.0 to 12.0 micromol/L, the inhibitory effect was in a concentration dependent manner. After treatment with 4.0 micromol/L GNA for 48 h, apoptosis was obviously observed as assayed by Annexin V-FITC/PI staining. Importantly, MMP was decreased and ROS formation was increased following GNA treatment. Additionally, P53 expression was up-regulated following 4.0 micromol/ L GNA treatment in a time dependent manner.

Conclusion: GNA induces mitochondria-dependent apoptosis and increases P53 expression in human gastric carcinoma cell line.
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January 2014

[Effect and mechanism of polyphyllin I on human cervical cancer cell HeLa in vitro].

Zhong Yao Cai 2013 Nov;36(11):1815-9

Objective: To study the effect and mechanism of polyphyllin I on human cervical cancer cell HeLa.

Methods: The cell growth and proliferation effect of Polyphyllin I on HeLa cells were measured by MTT assay; Hoechst 33258 fluorescent staining was used to record changes in cell morphology and morphological changes in mitochondria of Polyphyllin I before and after treatment on He La cells. Annexin V-FITC/PI staining was used to detect the ratio of tumor cell apoptosis by flow cytometry. Release of intracellular re active oxygen species (ROS) generation level in HeLa cells was determined by flow cytometry,Caspase-3 activity was measured by fluorescent assay kits.

Results: MTT results showed that Polyphyllin I could significantly suppressed the proliferation of HeLa cells and in time-and concentration-dependence manner. The intracellular ROS levels were increased dramatically and the mitochondrial membrane was decreased consistently. Caspase-3 proteins expression levels were increased after Polyphyllin I treatment.

Conclusion: Polyphyllin I could inhibit HeLa cells growth and proliferation and its mechanism may be related to inducing cell apoptosis.
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November 2013

Extracts from Curcuma zedoaria Inhibit Proliferation of Human Breast Cancer Cell MDA-MB-231 In Vitro.

Evid Based Complement Alternat Med 2014 5;2014:730678. Epub 2014 May 5.

Zhejiang Cancer Hospital, Zhejiang 310022, China.

Objective. To evaluate the effect of petroleum ether extracts of Curcuma zedoaria on the proliferation of human triple negative breast cancer cell line MDA-MB-231. Methods. The reagents were isolated from Curcuma zedoaria by petroleum ether fraction. It was assayed by CCK8 for MDA-MB-231 cellular viability with various concentrations and days, cell cycle analyses, Western Blot analysis, and Realtime Reverse Transcriptase PCR analyses for chemokines molecules including E-cadherin, and E-selectin, and adhesion molecules including CCR7, SLC, SDF-1, and CXCR4. Epirubicin was used as control in the study. Results. MDA-MB-231 cells were inhibited by petroleum ether extracts of Curcuma zedoaria (P < 0.05), and the inhibition rate was dependent on concentrations and time. Petroleum ether extracts of Curcuma zedoaria as well as Epirubicin produce a significant G0/G1 cell cycle arrest. The level of expression of proteins E-cadherin and E-cadherin mRNA was significantly increased, while proteins SDF-1, CCR7, and CXCR4 mRNA were decreased after being incubated with petroleum ether extracts of Curcuma zedoaria at the concentrations of 300  μ g/mL than control (P < 0.05). The differences were that the protein CXCR4 mRNA expression level was higher than vehicle. Conclusions. MDA-MB-231 cells were inhibited by petroleum ether extracts of Curcuma zedoaria.
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http://dx.doi.org/10.1155/2014/730678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4026840PMC
June 2014

Long non-coding RNAs in stem cells and cancer.

World J Clin Oncol 2014 May;5(2):134-41

Gabriel Eades, Yong-Shu Zhang, Qing-Lin Li, Ji-Xiang Xia, Yuan Yao, Qun Zhou, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, United States.

An overwhelming majority of the transcribed genome encodes for non-coding RNA (ncRNA) sequences. Deep sequencing of the transcriptome has uncovered tens of thousands of long ncRNA (lncRNA) sequences. However, little is known regarding the possible functions for a vast majority of these sequences. Among those lncRNAs whose function has been experimentally validated, most serve as regulators of gene expression. LncRNAs have been found to be critical to development and homeostasis and they have been implicated in several pathologies including cancer. Here, we examine the functions and underlying mechanisms of lncRNAs in stem cells and in cancer biology, areas linked by the actions of lncRNAs.
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http://dx.doi.org/10.5306/wjco.v5.i2.134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014785PMC
May 2014

[Effect of chaperone-mediated autophagy in MPP(+) -induced SH-SY5Y cells and interventional effect of puerarin].

Zhongguo Zhong Yao Za Zhi 2014 Jan;39(1):106-12

Objective: To study the protective effect of puerarin on MPP(+) -induced SH-SY5Y cells by chaperone-mediated autophagy (CMA).

Method: The Parkinson's disease cell model was established by injuring SH-SY5Y cells with 1 mmol x L(-1) MPP+. The CCK-8 staining was adopted to detect the effect the puerarin of different concentrations on the survival rate of MPP(+)-induced SH-SYSY cells. The autophagosome formation was observed under transmission electron microscope. The AO staining showed the changes in the lysosome activity. RT-PCR was used to detect the changes in Lamp2a and Hsc70 mRNA expressions. The western blotting was adopted to test the expressions of Lamp2a, Hsc70 and alpha-synuclein protein in cells.

Result: Within the concentration range of 12. 5-50.0 micromol x L(-1), the pretreatment with puerain for 30 minutes could protect the injury of MPP+ in SH-SY5Y cells, and showed a certain dose-effect relationship. The AO staining and electron microscope showed the effect of puerain within the concentration range of 12.5-50.0 micromol x L(-1) on 1 mmol x L(-1) MPP(+)-induced SH-SY5Y cells; autophagosomes emerged in cells, and increased along with the rise in the puerarin dose. The results of the flow cytometry revealed that 50.0 micromol x L(-1) of puerarin could protect against the increase of the ROS level in 1 mmol x L(-1) MPP(+) -induced SH-SY5Y cells and prevent the oxidative injury. The results of RT-PCR and western blotting indicated that puerain within the concentration range of 12.5-50.0 micromol x L(-1) alleviated the MPP(+)-induced SH-SY5Y cell injury, and inhibited the accumulation of alpha-synuclein proteins in MPP(+) -induced SH-SY5Y cells by up-regulating Hsc70, Lamp2a mRNA and protein level.

Conclusion: Puerarin could protect against the MPP(+) -induced cell injury, whose protective mechanism may be related to the chaperone-mediated autophagy pathway of interventional molecules.
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January 2014

[Risk factors and short-term prognosis of acute kidney injury in elderly patients].

Zhonghua Yi Xue Za Zhi 2013 Sep;93(34):2715-8

Department of Geriatric Nephrology, Chinese PLA General Hospital, Beijing 100853, China.

Objective: To explore the risk factors and short-term outcomes of acute kidney injury (AKI) in elderly patients.

Methods: A total of 232 elderly AKI patients at Chinese PLA General Hospital from June 2008 to December 2009 were enrolled. They were divided into two groups according to their outcomes at 28 days and at 29 days to 3 months after AKI respectively. Their clinical data were analyzed to explore the risk factors and their effects on the outcomes of AKI.

Results: There were 215 males and 17 females with an average age of (86.7 ± 5.3) years. Thirty-eight cases (16.4%) died within 28 days after AKI and 57 (24.6%) died within 3 months. Infection (43.1%) was the major cause of AKI. The other causes included hypovolemia (19.0%), use of nephrotoxic drugs (16.8%) and cardiovascular events (15.1%) respectively. Logistic regression analysis revealed that low body mass index (BMI), oliguria, mechanical ventilation, hypoalbuminemia and peak serum level of creatinine (Scr >246.5 µmol/L) were the prognostic factors of AKI in those patients dying within 28 days after AKI (P < 0.05). Low BMI, hypoalbuminemia and high blood level of urea nitrogen (BUN) were the prognostic factors of AKI in those patients dying within 29 days to 3 months after AKI (P < 0.05).

Conclusion: Infection, hypovolemia, use of nephrotoxic drugs and cardiovascular events are common causes of AKI in elderly patients.Low BMI, oliguria, mechanical ventilation, hypoalbuminemia, high level of BUN and peak level of Scr ( > 246.5 µmol/L) are the prognostic factors of AKI in elderly patients.
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September 2013

The characteristics of Chinese orthographic neighborhood size effect for developing readers.

PLoS One 2012 8;7(10):e46922. Epub 2012 Oct 8.

Key Laboratory of Behavioral Science, Institute of Psychology, Chinese Academy of Sciences, Beijing, China.

Orthographic neighborhood size (N size) effect in Chinese character naming has been studied in adults. In the present study, we aimed to explore the developmental characteristics of Chinese N size effect. One hundred and seventeen students (40 from the 3(rd) grade with mean age of 9 years; 40 from the 5(th) grade with mean age of 11 years; 37 from the 7(th) grade with mean age of 13 years) were recruited in the study. A naming task of Chinese characters was adopted to elucidate N-size- effect development. Reaction times and error rates were recorded. Results showed that children in the 3(rd) grade named characters from large neighborhoods faster than named those from small neighborhoods, revealing a facilitatory N size effect; the 5(th) graders showed null N size effect; while the 7(th) graders showed an inhibitory N size effect, with longer reaction times for the characters from large neighborhoods than for those from small neighborhoods. The change from facilitation to inhibition of neighborhood size effect across grades suggested the transition from broadly tuned to finely tuned lexical representation in reading development, and the possible inhibition from higher frequency neighbors for higher graders.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046922PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3466200PMC
May 2013

Solid lipid nanoparticles as delivery systems for Gambogenic acid.

Colloids Surf B Biointerfaces 2013 Feb 7;102:391-7. Epub 2012 Sep 7.

Department of Clinical Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230038, China.

In this study, Gambogenic acid loaded by solid lipid nanoparticles (GNA-SLNs) was explored to reduce toxicity and improve therapeutic efficacy. GNA-SLNs were prepared by emulsification and low temperature solidification methods, and the freeze-dried powders were then developed to improve the stability. The physical-chemical properties of the products in terms of particle size, zeta potential, morphology and entrapment efficiency were well evaluated. The results revealed that the mean diameter, polydispersivity index (PI), zeta potential, and the entrapment efficiency of the nanoparticles were 163.3 nm, 0.203, -16.9 mV and 61.2%, respectively. In comparion with GNA-SLNs, the freeze-dried solid lipid nanoparticles (SLNs) showed a slight augmentation in the mean particle size (from 163.3 to 173 nm) and PI (from 0.203 to 0.253), and no significant modification in the zeta potential, entrapment efficiency and drug loading. In vitro release kinetics based on a dialysis method demonstrated that Gambogenic acid (GNA) was released in a prolonged fashion for 96 h and followed Higuchi equation unitarily. The release profile did not show any significant modification after the freeze-drying process. The Pharmacokinetic study was carried out, the i.p. administration of GNA formulations to rats at doses of 2.5mg/kg. AUC((0-t)) was increased (up to 3.1-fold) and clearance was decreased (up to 3.03-fold) when GNA entrapped in SLNs. In conclusions, the freeze-dried powders form could enhance the long-term stability of SLN, and solid lipid nanoparticles encapsulation could effectively strategy to change the poor aqueous solubility and prolong the half-life of GNA.
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http://dx.doi.org/10.1016/j.colsurfb.2012.08.058DOI Listing
February 2013

[Nondestructive analysis of a Liuli ring of Warring State Period by EDXRF probe].

Guang Pu Xue Yu Guang Pu Fen Xi 2011 Dec;31(12):3395-8

History School of Wuhan University, Wuchang 430072, China.

A Liuli ring was nondestructively analyzed by EDXRF probe, to discuss the characteristic of chemical composition and the producing place of this Liuli ring. The result reveals that the chemical composition of this Liuli ring belongs to PbO-BaO-SiO2 system, which is similar to Chinese special ancient PbO-BaO-SiO2 glass dating from Chunqiu to East Han state. So, it was concluded that this Liuli ring was used Chinese special technology and formula, and was made in China. But on the appearance, this Liuli ring is not like a mature glass, but a faience or frit which is the compound of glass and crystal substance.
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December 2011

Neural basis of phonological processing in second language reading: an fMRI study of Chinese regularity effect.

Neuroimage 2012 Mar 8;60(1):419-25. Epub 2012 Jan 8.

Key Laboratory of Behavioral Science, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China.

The present study examined the neural basis of phonological processing in Chinese later acquired as a second language (L2). The regularity effect of Chinese was selected to elucidate the addressed phonological processing. We recruited a group of alphabetic language speakers who had been learning Chinese as L2 for at least one year, and a control group of native Chinese speakers. Participants from both groups exhibited a regularity effect in a pilot behavioral test. Neuroimaging results revealed that L2 learners exhibited stronger activation than native Chinese speakers in the right occipitotemporal region (i.e. right lingual gyrus and right fusiform gyrus). Moreover, L2 learners exhibited greater activations in the ventral aspects of the left inferior parietal lobule (LIPL) and the left inferior frontal gyrus (LIFG) for irregular character reading minus regular character reading. In contrast, native Chinese speakers exhibited more dorsal activations in the LIPL and LIFG. According to the "accommodation/assimilation" hypothesis of second language reading, the current findings suggest that native speakers of alphabetic languages utilized an accommodation pattern for the specific requirements of the visual form of Chinese characters, and an assimilation pattern for orthography-to-phonology transformation in Chinese reading.
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http://dx.doi.org/10.1016/j.neuroimage.2011.12.074DOI Listing
March 2012

Gambogenic acid inhibits proliferation of A549 cells through apoptosis inducing through up-regulation of the p38 MAPK cascade.

J Asian Nat Prod Res 2011 Oct 19;13(11):993-1002. Epub 2011 Oct 19.

Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui Province Key Laboratory of R&D of Traditional Chinese Medicine, Anhui University of Traditional Chinese Medicine, Hefei, 230038, China.

Gamboge is a dry resin secreted from Garcinia hanburryi, and gambogenic acid (GNA) is one of the main active compounds of gamboge. We have previously demonstrated the anticancer activity of GNA in A549 cells and pointed out its potential effects in anticancer therapies. Previous studies reported that GNA induced apoptosis in many cancer cell lines and inhibited A549 tumor growth in xenograft of nude mice in vivo. However, the anticancer mechanism of GNA has still not been well studied. In this paper, we have investigated whether GNA-induced apoptosis is critically mediated by the p38 mitogen-activated protein kinase (MAPK) pathway. Our findings revealed that GNA could induce apoptosis, inhibit proliferation, down-regulate the expression of p38 and MAPK, increase the activations of caspase-9, caspase-3, and cytochrome c release. Furthermore, using SB203580, an adenosine triphosphate-competitive inhibitor of p38 MAPK, inhibit the expression of p-p38 and the experimental results show that it may promote the occurrence of apoptosis induced by GNA. Taken together, these results suggested that up-regulation of the p38 MAPK cascade may account for the activation of GNA-induced apoptosis.
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http://dx.doi.org/10.1080/10286020.2011.605062DOI Listing
October 2011

Activin B promotes epithelial wound healing in vivo through RhoA-JNK signaling pathway.

PLoS One 2011 19;6(9):e25143. Epub 2011 Sep 19.

Department of Histology and Embryology, Southern Medical University, Guangzhou, China.

Background: Activin B has been reported to promote the proliferation and migration of keratinocytes in vitro via the RhoA-JNK signaling pathway, whereas its in vivo role and mechanism in wound healing process has not yet been elucidated.

Principal Findings: In this study, we explored the potential mechanism by which activin B induces epithelial wound healing in mice. Recombinant lentiviral plasmids, with RhoA (N19) and RhoA (L63) were used to infect wounded KM mice. The wound healing process was monitored after different treatments. Activin B-induced cell proliferation on the wounded skin was visualized by electron microscopy and analyzed by 5'-bromodeoxyuridine (BrdU) incorporation assay. Protein expression of p-JNK or p-cJun was determined by immunohistochemical staining and immunoblotting analysis. Activin B efficiently stimulated the proliferation of keratinocytes and hair follicle cells at the wound area and promoted wound closure. RhoA positively regulated activin B-induced wound healing by up-regulating the expression of p-JNK and p-cJun. Moreover, suppression of RhoA activation delayed activin B-induced wound healing, while JNK inhibition recapitulated phenotypes of RhoA inhibition on wound healing.

Conclusion: These results demonstrate that activin B promotes epithelial wound closure in vivo through the RhoA-Rock-JNK-cJun signaling pathway, providing novel insight into the essential role of activin B in the therapy of wound repair.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0025143PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3176320PMC
February 2012

[Nondestructive analysis of some ancient glass in Jin and Yuan dynasty, by EDXRF probe].

Guang Pu Xue Yu Guang Pu Fen Xi 2011 Jul;31(7):1960-3

History School of Wuhan University, Wuchang 430072, China.

The chemical composition of some glass samples in Jin and Yuan Dynasty were analyzed by EDXRF, to discuss the technological character of these glass samples. The result shows that the chemical composition of these glass samples is very complex. According to the chemical composition, these glass samples include K2-O-CaO-SiO2, Na2O-CaO-SiO2 and K2O-CaO-PbO-SiO2 glass, and a crystal SiO2 sample which was once considered a glass sample. The reason for the color of all blue glass samples is the existence of CuO, but not CoO.
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July 2011
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