Publications by authors named "Qing Zhou"

1,251 Publications

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Design, Synthesis and Antibacterial Activity of Novel Pyrimidine-Containing 4H-Chromen-4-one Derivatives.

Chem Biodivers 2021 Jun 22. Epub 2021 Jun 22.

Ministry of Education, State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Guizhou University, 550025, Guiyang, CHINA.

A series of pyrimidine-containing 4H-chromen-4-one derivatives were designed and synthesized by combining bioactive substructures. Preliminary biological activity results showed that most of the compounds displayed significant inhibitory activities in vitro against Xanthomonas axonopodis pv. Citri ( X. axonopodis), Xanthomonas oryzae pv. oryzae (X. oryzae) and Ralstonia solanacearum (R. solanacearum). In particular, compound 2-((3-((5,7-dimethoxy-4-oxo-2-(3,4,5-trimethoxyphenyl)-4H-chromen-3-yl)oxy)propyl)thio)-6-oxo-4-(p-tolyl)-1,6-dihydropyrimidine-5-carbonitrile ( 4c ) demonstrated a good inhibitory effect against X. axonopodis and X. oryzae, with the half-maximal effective concentration(EC 50 ) values of 15.5 and 14.9 μg/mL, respectively, and compound 2-((3-((5,7-dimethoxy-4-oxo-2-(3,4,5-trimethoxyphenyl)-4H-chromen-3-yl)oxy)propyl)thio)-4-(3-fluorophenyl)-6-oxo-1,6-dihydropyrimidine-5-carbonitrile ( 4h ) showed the best antibacterial activity against R. solanacearum with an EC50 value of 14.7 μg/mL. These results were better than commercial reagents bismerthiazol (BT, 51.7, 70.1 and 52.7 μg/mL, respectively) and thiodiazole copper (TC, 77.9, 95.8 and 72.1 μg/mL respectively). In vivo antibacterial activity results indicated that compound 2-((3-((5,7-dimethoxy-4-oxo-2-(3,4,5-trimethoxyphenyl)-4H-chromen-3-yl)oxy)propyl)thio)-6-oxo-4-(p-tolyl)-1,6-dihydropyrimidine-5-carbonitrile (4c) displayed better curative(42.4%) and protective (49.2%) activities for rice bacterial leaf blight than BT (35.2, 39.1%) and TC (30.8, 27.3%). The mechanism of compound 2-((3-((5,7-dimethoxy-4-oxo-2-(3,4,5-trimethoxyphenyl)-4H-chromen-3-yl)oxy)propyl)thio)-6-oxo-4-(p-tolyl)-1,6-dihydropyrimidine-5-carbonitrile ( 4c ) against X. oryzae was analysed through scanning electron microscopy (SEM).  These results indicated that pyrimidine-containing 4H-chromen-4-one derivatives have important value in the research of new agrochemicals.
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http://dx.doi.org/10.1002/cbdv.202100186DOI Listing
June 2021

Low-dose lanthanum activates endocytosis, aggravating accumulation of lanthanum or/and lead and disrupting homeostasis of essential elements in the leaf cells of four edible plants.

Ecotoxicol Environ Saf 2021 Jun 17;221:112429. Epub 2021 Jun 17.

National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China. Electronic address:

Rare earth elements (REEs) are emerging as a serious threat to ecological safety due to their increasing accumulation in environments. The accumulation of REEs in environments has significantly increased its accumulation in the leaves of edible plants. However, the accumulation pathway of REEs in the leaves of edible plants are still unknown. In this study, lanthanum [La(III), a widely used and accumulated REE] and four edible plants (soybean, lettuce, pakchoi, and celery) with short growth cycles were selected as research objects. By using interdisciplinary research techniques, we found that low-dose La(III) activated endocytosis (mainly the clathrin-mediated endocytosis) in the leaf cells of four edible plants, which provided an accumulation pathway for low-dose La in the leaf cells of these edible plants. The accumulation of La in the leaf cells was positively correlated with the intensity of endocytosis, while the intensity of endocytosis was negatively correlated with the density of leaf trichomes. In addition to the accumulation of La, low-dose La(III) also brought other risks. For example, the harmful element (Pb) can also be accumulated in the leaf cells via La(III)-activated endocytosis; the homeostasis of the essential elements (K, Ca, Fe, Mg) was disrupted, although the chlorophyll synthesis and the growth of these leaf cells were accelerated; and the expression of stress response genes (GmNAC20, GmNAC11) in soybean leaves was increased. These results provided an insight to further analyze the toxicity and mechanism of REEs in plants, and sounded the alarm for the application of REEs in agriculture.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112429DOI Listing
June 2021

Association of serum selenium with anemia-related indicators and risk of anemia.

Food Sci Nutr 2021 Jun 27;9(6):3039-3047. Epub 2021 Mar 27.

Science and Education Department Shenzhen Baoan Shiyan People's Hospital Shenzhen China.

Few studies have examined the association of serum selenium with anemia-related indicators and risk of anemia. We conducted a cross-sectional analysis of 2,902 adults in 2003-2004 National Health and Nutrition Examination Survey database. Multivariable linear and logistic regression models were used to examine the association of serum selenium with anemia-related indicators and risk of anemia. The nonlinear relationship was analyzed using a generalized additive model with the smoothing plot. A total of 1,472 males and 1,430 females with a mean age of 61.94 ± 13.73 years were included. Compared with the lowest quintile, the highest quintile of serum selenium was associated with increased level of serum iron (β = 12.44, 95% confidence interval [CI]: 7.14, 17.75,  < .001), mean corpuscular hemoglobin concentration (MCHC) (β = 0.14, 95%CI: 0.02, 0.26,  = .020), and hemoglobin (β = 0.40, 95%CI: 0.19, 0.61,  < .001), and decreased risk of anemia (odds ratio [OR] = 0.47, 95%CI: 0.28, 0.77,  = .002). Furthermore, smoothed plots suggested the nonlinear relationships between serum selenium and MCHC, hemoglobin level, and risk of anemia. Interestingly, on the left of inflection point, serum selenium was associated with decreased risk of anemia (OR = 0.972, 95%CI: 0.960, 0.985,  < .001), and then, the risk of anemia increased with increasing serum selenium concentration (OR = 1.011, 95%CI: 1.002, 1.021,  = .023). Future large-scale, polycentric prospective studies should be conducted to verify our results.
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http://dx.doi.org/10.1002/fsn3.2261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194763PMC
June 2021

Two New Coordination Polymers: Selective Detection of Cu(II) Ion and Treatment Activity on Inner Ear Damage.

J Fluoresc 2021 Jun 15. Epub 2021 Jun 15.

Department of Medicine, Shenzhen University, Shenzhen, Guangdong, China.

In the current study, on the basis of 1,3,5-tris(2-methylimidazol-1-yl)benzene (timb), a designed tripodal connector, two new transition metal coordination polymers (CPs), {[Cu(timb)(Br-IPA)]·5HO} (1) and {[Zn(timb)(NH-IPA)]·4HO} (2) have been generated with the mixed ligand method by the reaction between the timb and corresponding metal salts in the existence of dissimilar functional isophthalic acid (HIPA) ligands. Furthermore, the Zn(II)-based complex 2 displays high sensitivity in the detection of Cu(II) ion in water. The neural stem cells proliferation after treated via compounds was detected with Cell Counting Kit-8 detection assay. And the real time reverse transcription polymerase chain reaction was carried out for the investigation of the differentiation function of the neural stem cells after the compound 1 treatment and compound 2 treatment. Further, molecular docking simulations confirmed that the biological activity that has been observed from experiments were from the carboxyl group on the Cu complex, in contrast, the imidazole groups were only used for binding with the Cu metal ion to retain the complex structure.
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http://dx.doi.org/10.1007/s10895-021-02768-3DOI Listing
June 2021

A clustering-triggered emission strategy for tunable multicolor persistent phosphorescence.

Chem Sci 2020 Feb 11;11(11):2926-2933. Epub 2020 Feb 11.

School of Chemistry and Chemical Engineering, Shanghai Key Lab of Electrical Insulation and Thermal Aging, Shanghai Electrochemical Energy Devices Research Center, Shanghai Jiao Tong University Shanghai 200240 China

A clustering-triggered emission (CTE) strategy, namely the formation of heterogeneous clustered chromophores and conformation rigidification, for achieving tunable multicolor phosphorescence in single-component compounds is proposed. Non-conventional luminophores comprising just oxygen functionalities and free of π-bonding, , d-(+)-xylose (d-Xyl), pentaerythritol (PER), d-fructose (d-Fru) and d-galactose (d-Gal), were adopted as a simple model system with an explicit structure and molecular packing to address the hypothesis. Their concentrated solutions and crystals at 77 K or under ambient conditions demonstrate remarkable multicolor phosphorescence afterglows in response to varying excitation wavelengths, because of the formation of diverse oxygen clusters with sufficiently rigid conformations. The intra- and inter-molecular O⋯O interactions were definitely illustrated by both single crystal structure analysis and theoretical calculations. These findings shed new light on the origin and simple achievement of tunable multicolor phosphorescence in single-component pure organics, and in turn, have strong implications for the emission mechanism of non-conventional luminophores.
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http://dx.doi.org/10.1039/c9sc06518kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157572PMC
February 2020

QTMP, a Novel Thiourea Polymer, Causes DNA Damage to Exert Anticancer Activity and Overcome Multidrug Resistance in Colorectal Cancer Cells.

Front Oncol 2021 28;11:667689. Epub 2021 May 28.

School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.

Colorectal cancer (CRC) is one of the most common malignancies, and multidrug resistance (MDR) severely restricts the effectiveness of various anticancer drugs. Therefore, the development of novel anticancer drugs for the treatment of CRC patients with MDR is necessary. Quaternized thiourea main-chain polymer (QTMP) is a self-assembled nanoparticle with good water solubility. Notably, QTMP is not a P-glycoprotein (P-gp) substrate, and it exhibits potent cytotoxic activity against CRC cells, including HCT116/DDP and P-gp-mediated multidrug-resistant Caco2 cells. QTMP also exhibits a strong anticancer activity against SW480 cells . Interestingly, reactive oxygen species (ROS) and reactive nitrogen species (RNS) production were increased in a concentration-dependent manner in QTMP-treated HCT116, SW480 and Caco2 cells. Importantly, QTMP causes DNA damage in these CRC cells direct insertion into the DNA or regulation of ROS and/or RNS production. QTMP also induces caspase-dependent apoptosis overproduction of ROS and RNS. Therefore, QTMP is a promising anticancer therapeutic agent for patients with CRC, including those cancer cells with P-gp-mediated MDR. The present study also indicates that the design and synthesis of anticancer drugs based on thiourea polymers is promising and valuable, thereby offering a new strategy to address MDR, and provides reference resources for further investigations of thiourea polymers.
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http://dx.doi.org/10.3389/fonc.2021.667689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194350PMC
May 2021

Comparison of viscocanalostomy plus suture-assisted near-360-degree trabeculotomy and viscocanalostomy plus rigid probe trabeculotomy in primary congenital glaucoma.

Acta Ophthalmol 2021 Jun 11. Epub 2021 Jun 11.

Department of Ophthalmology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Purpose: To compare the efficacy and safety of viscocanalostomy plus near-360-degree suture trabeculotomy (VST) with viscocanalostomy plus rigid probe trabeculotomy (VT) in treating primary congenital glaucoma (PCG) over a one-year follow-up.

Methods: This consecutive retrospective study included patients with PCG confirmed within 3 years of age from March 2017 to October 2019. Efficacy was evaluated by comparing the postoperative intraocular pressure (IOP) curve and the success rate at one year after surgery. Safety was assessed by comparing the postoperative complications. The number of anti-glaucoma agents, horizontal corneal diameter (HCD) and cup-to-disc ratio (C/D) of the two surgical methods were also compared.

Results: Data of 90 eyes from 61 patients were analysed. The baseline parameters of the two groups were similar. The IOP at 12 months after surgery in the VST group was 12.7 ± 4.8 mmHg, while that in the VT group was 15.8 ± 6.5 mmHg. The IOP at 6, 9 and 12 months postoperatively in the VST group was significantly lower than in the VT group (p < 0.05). Viscocanalostomy plus near-360-degree suture trabeculotomy (VST) remained a significant favourable factor for complete one-year success (93.6% versus 74.4%, p = 0.005) but not qualified one-year success (97.9% versus 88.4%, p = 0.06). The number of anti-glaucoma agents, HCD and C/D were reduced in both groups. Postoperative complications were not significantly different between the two groups.

Conclusions: In children with PCG, VST provides a more durable IOP control than VT over the one-year follow-up, with a similar safety profile.
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http://dx.doi.org/10.1111/aos.14941DOI Listing
June 2021

Heart Failure With Mid-range Ejection Fraction: A Distinctive Subtype or a Transitional Stage?

Front Cardiovasc Med 2021 25;8:678121. Epub 2021 May 25.

State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Heart failure with mid-range ejection fraction (HFmrEF) was first proposed by Lam and Solomon in 2014, and was listed as a new subtype of heart failure (HF) in 2016 European Society of Cardiology guidelines. Since then, HFmrEF has attracted an increasing amount of attention, and the number of related studies on this topic has grown rapidly. The diagnostic criteria on the basis of left ventricular ejection fraction (LVEF) are straightforward; however, LVEF is not a static parameter, and it changes dynamically during the course of HF. Thus, HFmrEF may not be an independent disease with a uniform pathophysiological process, but rather a collection of patients with different characteristics. HFmrEF is often associated with various cardiovascular and non-cardiovascular diseases. Thus, the pathophysiological mechanisms of HFmrEF are particularly complex, and its clinical phenotypes are diverse. The complexity and heterogeneity of HFmrEF may be one reason for inconsistent results between clinical studies. In fact, whether HFmrEF is a distinctive subtype or a transitional stage between HF with reduced ejection fraction (HFrEF) and HF with preserved ejection fraction (HFpEF) is controversial. In this review, we discuss the clinical characteristics, treatment and prognosis of patients with HFmrEF, as well as the differences among HFmrEF, HFrEF, and HFpEF.
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http://dx.doi.org/10.3389/fcvm.2021.678121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185203PMC
May 2021

Utilization of Echocardiography After Acute Kidney Injury Was Associated with Improved Outcomes in Patients in Intensive Care Unit.

Int J Gen Med 2021 1;14:2205-2213. Epub 2021 Jun 1.

Department of Ultrasound Imaging, Renmin Hospital of Wuhan University, Wuhan, 430061, People's Republic of China.

Background: We aimed to investigate the association between usage of transthoracic echocardiography (TTE) within 24 hours after acute kidney injury (AKI) and the prognosis of patients in intensive care unit (ICU).

Methods: The Medical Information Mart for Intensive Care III (MIMIC-III) database was used to identify AKI patients with and without TTE administration. The primary outcome was 28-day mortality. Multivariable regression was used to clarify the association between TTE and clinical outcomes and propensity score matching (PSM) and inverse probability of treatment weighting (IPTW) were utilized to validate our findings.

Results: Among 23,945 eligible AKI patients, 3361 patients who received TTE and 3361 who did not conduct TTE had similar propensity scores which were included in this study. After matching, the TTE group had a significantly lower 28-day mortality (OR 0.80, 95% CI 0.72-0.88, P<0.001). Patients in the TTE group received more fluid on day 1 and day 2 and had a more urine volume on day 1 and day 3, and the reduction in serum creatinine was greater than that in the no TTE group. The mediating effect of creatinine reduction was remarkable for the whole cohort (P=0.02 for the average causal mediation effect).

Conclusion: TTE utilization was associated with decreased risk-adjusted 28-day mortality for AKI patients in ICU and was proportionally mediated through creatinine reduction.
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http://dx.doi.org/10.2147/IJGM.S310445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183456PMC
June 2021

Early prediction of response to neoadjuvant chemotherapy using contrast-enhanced ultrasound in breast cancer.

Medicine (Baltimore) 2021 May;100(19):e25908

Department of Medical Ultrasound.

Abstract: Early prediction of non-response is essential in order to avoid inefficient treatments. The objective of this study was to determine the contrast-enhanced ultrasound (CEUS) for early predicting pathologic complete response (pCR) to neoadjuvant chemotherapy (NAC) in breast cancer patients.Between March 2018 and October 2019, 93 consecutive patients with histologically proven breast cancer scheduled for NAC were enrolled. Conventional ultrasound and CEUS imaging were performed before NAC and after two cycles of NAC. CEUS parameters were compared with pathologic response. Multiple logistic regression analyses were utilized to explore CEUS parameters to predict pCR, and receiver operating characteristic analysis was used to evaluate the predictive ability.Therapeutic response was obtained from 25 (27%) patients with pCR and 68 (73%) with non-pCR. Compared to non-pCR, pCR cases have a significantly higher proportion of homogeneous enhancement feature (56% vs 14%, P < .001) and centripetal enhancement (52% vs 23%, P = .012). A significant decrease in peak intensity (PI) was observed after two cycles of NAC. Compared with non-pCR patients, the kinetic parameters PI change (PI%) was higher in pCR patients (P < .001). Multiple logistic regression demonstrated two independent predictors of pCR: internal homogeneity (odds ratio, 4.85; 95% confidence interval: 1.20-19.65; P = .027) and PI% (odds ratio, 1.08; 95% confidence interval: 1.02-1.15; P = .007). In receiver operating characteristic curve analysis, internal homogeneity and PI%, with area under curve of 0.71 and 0.84, predicted pCR with sensitivity (56%, 95%) and specificity (85%, 70%), respectively.Internal homogeneity and PI% of CEUS may be useful in the noninvasive early prediction of pCR in patients with breast cancer.
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http://dx.doi.org/10.1097/MD.0000000000025908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133101PMC
May 2021

[A review of consensus statements, practice resources, standards and guidelines for clinical applications of next-generation sequencing technologies in the United States].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Jun;38(6):513-520

Department of Genetics, Yale University School of Medicine, New Haven CT 06511, USA.

The use of whole exome sequencing (WES) for the detection of disease-causing variants of genetic diseases and for non-invasive prenatal screening (NIPS) of fetal aneuploidies are two major clinical applications of next generation sequencing (NGS). This article has summarized the official documents developed and updated by the American College of Medical Genetics and Genomics (ACMG) on governing WES and NIPS. These include the development of expert consensus policies and position statements on an ongoing basis to guide clinical application of NGS technology and variant analysis, establish evidence-based practical resources, as well as standards and guidelines to govern diagnosis and screening. These ACMG documents are valuable references to Chinese geneticists, but direct adoption of these standards and guidelines may not be practical due to the differences in disease-associated variant frequencies in Chinese population, socioeconomic status, and medical practice between the two countries. It is hoped that this review could facilitate the development of NGS and NIPS standards and guidelines that are consistent with international standards and concordant with medical genetics practice in China to provide high-quality, efficient and safe clinical services for patients and their families with genetic diseases.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200924-00691DOI Listing
June 2021

Local administration of liposomal-based Srpx2 gene therapy reverses pulmonary fibrosis by blockading fibroblast-to-myofibroblast transition.

Theranostics 2021 13;11(14):7110-7125. Epub 2021 May 13.

Department of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of National Health Commission, Key Site of National Clinical Research Center for Respiratory Disease, Wuhan Clinical Medical Research Center for Chronic Airway Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences and Technology, 1095 Jiefang Ave, Wuhan 430030, China.

Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive fatal interstitial lung disease characterized by abnormal transition and proliferation of fibroblasts. The uncontrolled transition of fibroblasts, commonly known as myofibroblasts, are the principal source of the enormous extracellular matrix (ECM) depositing in lung parenchyma, leading to gradual failure of gas exchange and mortality of the patients. However, up to now, rare effective therapeutic strategies have been developed to blockade fibroblast-to-myofibroblast transition (FMT) in IPF. We illustrated that the lungs originated from IPF patients and mice with pulmonary fibrosis are characterized by the overexpression of sushi-repeat-containing protein, X-linked 2 (SRPX2). Further functionality studies identified the pivotal role of SRPX2 in FMT. Mechanistically, SRPX2 was involved in a TGFβR1/SMAD3/SRPX2/AP1/SMAD7 positive feedback loop. Specifically, SRPX2 was upregulated by TGF-β1 in a TGFβR1/SMAD3-dependent manner, after which SRPX2 in turn repressed the expression of AP1, subsequently minimized SMAD7 expression, through which it reduced the formation of inhibitory complex with TGFβR1 and enhanced SMAD signaling pathway to promote FMT and exacerbate pulmonary fibrosis. Notably, intratracheal administration of siRNA-loaded liposomes could effectively suppress the expression of Srpx2 in the lung and remarkably protect mice against BLM-induced pulmonary fibrosis, concomitant with a significant reduction of FMT. Accordingly, these data indicate that Srpx2 plays an essential role in the pathogenesis of pulmonary fibrosis and suggests the strategy aiming at silencing Srpx2 could be a promising therapeutic approach against pulmonary fibrosis in clinical settings.
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http://dx.doi.org/10.7150/thno.61085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171094PMC
May 2021

Cell-Penetrating CEBPB and CEBPD Leucine Zipper Decoys as Broadly Acting Anti-Cancer Agents.

Cancers (Basel) 2021 May 20;13(10). Epub 2021 May 20.

Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.

Transcription factors are key players underlying cancer formation, growth, survival, metastasis and treatment resistance, yet few drugs exist to directly target them. Here, we characterized the in vitro and in vivo anti-cancer efficacy of novel synthetic cell-penetrating peptides (Bpep and Dpep) designed to interfere with the formation of active leucine-zipper-based dimers by CEBPB and CEBPD, transcription factors implicated in multiple malignancies. Both peptides similarly promoted apoptosis of multiple tumor lines of varying origins, without such effects on non-transformed cells. Combined with other treatments (radiation, Taxol, chloroquine, doxorubicin), the peptides acted additively to synergistically and were fully active on Taxol-resistant cells. The peptides suppressed expression of known direct CEBPB/CEBPD targets , and asparagine synthetase (), supporting their inhibition of transcriptional activation. Mechanisms by which the peptides trigger apoptosis included depletion of pro-survival survivin and a required elevation of pro-apoptotic BMF. Bpep and Dpep significantly slowed tumor growth in mouse models without evident side effects. Dpep significantly prolonged survival in xenograft models. These findings indicate the efficacy and potential of Bpep and Dpep as novel agents to treat a variety of cancers as mono- or combination therapies.
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http://dx.doi.org/10.3390/cancers13102504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161188PMC
May 2021

Ion-Exchange Resins for Efficient Removal of Colorants in Bis(hydroxyethyl) Terephthalate.

ACS Omega 2021 May 29;6(18):12351-12360. Epub 2021 Apr 29.

Beijing Key Laboratory of Ionic Liquids Clean Process, CAS Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, P. R. China.

Bis(hydroxyethyl) terephthalate (BHET) obtained from waste poly(ethylene terephthalate) (PET) glycolysis often have undesirable colors, leading to an increased cost in the decoloration of the product and limiting the industrialization of chemical recycling. In this work, eight types of ion-exchange resins were used for BHET decoloration, and resin D201 showed an outstanding performance not only in the decoloration efficiency but also in the retention rate of the product. Under the optimal conditions, the removal rate of the colorant and the retention efficiency of BHET were over 99% and 95%, respectively. D201 showed outstanding reusability with five successive cycles, and the decolored BHET and its r-PET showed good chromaticity. Furthermore, the investigations of adsorption isotherms, kinetics, and thermodynamics have been conducted, which indicated that the decoloration process was a natural endothermic reaction. Adsorption interactions between the colorant and resin were extensively examined by various characterizations, revealing that electrostatic force, π-π interactions, and hydrogen bonding were the dominant adsorption mechanisms.
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http://dx.doi.org/10.1021/acsomega.1c01477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154176PMC
May 2021

Exploring the Mechanism of Zhibai Dihuang Decoction in the Treatment of Ureaplasma Urealyticum-Induced Orchitis Based on Integrated Pharmacology.

Front Pharmacol 2021 10;12:602543. Epub 2021 May 10.

College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China.

Ureaplasma urealyticum (UU) infection is the most common cause of male infertility. Zhibai Dihuang Decoction (ZBDHD) can improve the rate of forwarding motility sperm, sperm deformity rate, seminal plasma zinc and refined berry sugar levels. The potential targets of ZBDHD are obtained from The Encyclopedia of Traditional Chinese Medicine (ETCM). Orchitis-related targets were collected from the Genecards and OMIM databases. The Cytoscape and the Database for Annotation, Visualization and Integrated Discovery (DAVID) were utilized to construct and analyzed the networks. Finally, a rat model of orchitis caused by UU infection was used to detect related indicators of mitochondrial energy metabolism using TUNEL apoptosis detection technology, loss cytometry, Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) and Western Blot. A total of 795 ZBDHD targets and 242 orchitis-related targets were obtained. The "ZBDHD- orchitis PPI network" was constructed and analyzed. ZBDHD can regulate signaling pathways and biological processes related to mitochondrial energy metabolism. The results of experimental studies have shown that ZBDHD maintains the integrity of sperm mitochondrial respiratory chain function by enhancing mitochondrial Na-K-ATPase and Ca2-Mg-ATPase activities, promotes the synthesis of mitochondrial ATP, and improves sperm energy supply, thereby improving the motility, vitality and survival rate of sperm, and effectively improving the quality of semen in UU-infected rats ( < 0.05). This study discovered the multi-pathway mechanism of ZBDHD intervention in UU-induced orchitis through integrated pharmacological strategies, which provides a reference for further research on the mechanism of ZBDHD intervention in orchitis in the direction of mitochondrial energy metabolism.
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http://dx.doi.org/10.3389/fphar.2021.602543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141734PMC
May 2021

Complicated Diagnosis and Treatment of HA20 due to Contiguous Gene Deletions involving 6q23.3.

J Clin Immunol 2021 May 25. Epub 2021 May 25.

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, 77030, USA.

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http://dx.doi.org/10.1007/s10875-021-01048-wDOI Listing
May 2021

The effect of accelerated rehabilitation nursing on postoperative recovery, nutritional status, and psychological status in patients with gastric cancer.

Am J Transl Res 2021 15;13(4):3666-3673. Epub 2021 Apr 15.

The Second Department of General Surgery, Affiliated Hospital of Jiangsu University Zhenjiang, Jiangsu Province, China.

Objective: To explore the effect of accelerated rehabilitation nursing in patients after gastric cancer surgery.

Methods: This prospective study included 88 gastric cancer patients who scheduled to receive surgery. According to the random number table, these patients were assigned to the control group and the experimental group. Patients in the control group received routine nursing, while those in the experimental group received accelerated rehabilitation nursing. Clinical-related parameters, nutritional index, physiological state, the quality of life (QOL), and complications were compared between the two groups.

Results: Compared with the control group, postoperative time to get out of bed, anal exhaust time, recovery time of bowel sound, and the length of hospitalization were shortened (all P<0.05). Hemoglobin (Hb), serum total protein (TP), and albumin (Alb) level in both groups after intervention were significantly lower than those before intervention (all P<0.05). Meanwhile, Hb, serum TP, and Alb level in the experimental group after intervention were significantly higher than those in the control group (all P<0.05). Visual analogue scale (VAS) scores at different time points (12 h-5 d after surgery) in the experimental group were significantly reduced when compared with the control group (all P<0.05). Hamilton anxiety scale (HAMA) and Hamilton depression scale (HAMD) score in the two groups after intervention were markedly lower those before intervention (both P<0.05). At the same time, HAMA and HAMD score in the experimental group after intervention were lower than those in the control group (both P<0.05). Generic quality of life inventory-74 (GQOLI-74) scores in all aspects after intervention were higher than those before intervention (all P<0.05). Meanwhile, GQOLI-74 scores in all aspects in the experimental group after intervention were higher than those in the control group (all P<0.05). The total incidence of complications in the experimental group was significantly decreased when compared with the control group (P<0.05).

Conclusion: For gastric cancer patients undergoing surgical treatment, accelerated rehabilitation nursing care can effectively promote their postoperative recovery of intestinal function, significantly improve their nutritional status, relieve their negative emotions, improve their quality of life, and reduce the incidence of complications. It is worth of clinical application.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129355PMC
April 2021

Targeted Inhibition of FTO Demethylase Protects Mice Against LPS-Induced Septic Shock by Suppressing NLRP3 Inflammasome.

Front Immunol 2021 4;12:663295. Epub 2021 May 4.

Department of Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, China.

Sepsis refers to the systemic inflammatory response syndrome caused by infection. It is a major clinical problem and cause of death for patients in intensive care units worldwide. The Fat mass and obesity-related protein (FTO) is the primary -methyladenosine demethylase. However, the role of FTO in the pathogenesis of inflammatory diseases remains unclear. We herein show that nanoparticle-mediated -siRNA delivery or FTO inhibitor entacapone administration dramatically inhibited macrophage activation, reduced the tissue damage and improved survival in a mouse model of LPS-induced endotoxic shock. Importantly, ablation of FTO could inhibit NLRP3 inflammasome through FoxO1/NF-κB signaling in macrophages. In conclusion, FTO is involved in inflammatory response of LPS-induced septic shock and inhibition of FTO is promising for the treatment of septic shock.
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http://dx.doi.org/10.3389/fimmu.2021.663295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128997PMC
May 2021

Comprehensive analysis of ADA2 genetic variants and estimation of carrier frequency driven by a function-based approach.

J Allergy Clin Immunol 2021 May 15. Epub 2021 May 15.

Division of Immunology, Boston Children's Hospital, Harvard Medical School, Boston, Mass; Division of Rheumatology, Inflammation, and Immunity, Brigham and Women's Hospital, Harvard Medical School, Boston, Mass. Electronic address:

Background: Deficiency of adenosine deaminase 2 (DADA2) is an autoinflammatory disease caused by deleterious ADA2 variants. The frequency of these variants in the general population, and hence the expected disease prevalence, remain unknown.

Objective: We aimed to characterize the functional impact and carrier frequency of ADA2 variants.

Methods: We performed functional studies and in silico analysis on 163 ADA2 variants, including DADA2-associated variants and population variants identified in the Genome Aggregation Database. We estimated the carrier rate using the aggregate frequency of deleterious variants.

Results: Functional studies of ADA2 variants revealed that 77 (91%) of 85 of DADA2-associated variants reduced ADA2 enzymatic function by >75%. Analysis of 100 ADA2 variants in the database showed a full spectrum of impact on ADA2 function, rather than a dichotomy of benign versus deleterious variants. We found several in silico algorithms that effectively predicted the impact of ADA2 variants with high sensitivity and specificity, and confirmed a correlation between the residual function of ADA2 variants in vitro and the plasma ADA2 activity of individuals carrying these variants (n = 45; r = 0.649; P < .0001). Using <25% residual enzymatic activity as the cutoff to define potential pathogenicity, integration of our results with the database population data revealed an estimated carrier frequency of at least 1 in 236 individuals, corresponding to an expected DADA2 disease prevalence of ~1 in 222,000 individuals.

Conclusions: Functional annotation guides the interpretation of ADA2 variants to create a framework that enables estimation of DADA2 carrier frequency and disease prevalence.
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http://dx.doi.org/10.1016/j.jaci.2021.04.034DOI Listing
May 2021

Vinegar/Tetramethylpyrazine Induces Nutritional Preconditioning Protecting the Myocardium Mediated by VDAC1.

Oxid Med Cell Longev 2021 20;2021:6670088. Epub 2021 Apr 20.

Institute of Cardiovascular Diseases, Jiangxi Academy of Clinical Medical Sciences, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.

Vinegar is good for health. Tetramethylpyrazine (TMP) is the main component of its flavor, quality, and function. We hypothesized that vinegar/TMP pretreatment could induce myocardial protection of "nutritional preconditioning (NPC)" by low-dose, long-term supplementation and alleviate the myocardial injury caused by anoxia/reoxygenation (A/R). To test this hypothesis, TMP content in vinegar was detected by HPLC; A/R injury model was prepared by an isolated mouse heart and rat cardiomyocyte to evaluate the myocardial protection and mechanism of vinegar/TMP pretreatment by many enzymatic or functional, or cellular and molecular biological indexes. Our results showed that vinegar contained TMP, and its content was in direct proportion to storage time. Vinegar/TMP pretreatment could improve hemodynamic parameters, decrease lactate dehydrogenase (LDH) and creatine phosphokinase activities, and reduce infarct size and apoptosis in the isolated hearts of mice with A/R injury. Similarly, vinegar/TMP pretreatment could increase cell viability, decrease LDH activity, and decrease apoptosis against A/R injury of cardiomyocytes. Vinegar/TMP pretreatment could also maintain the mitochondrial function of A/R-injured cardiomyocytes, including improving oxygen consumption rate and extracellular acidification rate, reducing reactive oxygen species generation, mitochondrial membrane potential loss, mitochondrial permeability transition pore openness, and cytochrome c releasing. However, the protective effects of vinegar/TMP pretreatment were accompanied by the downregulation of VDAC1 expression in the myocardium and reversed by pAD/VDAC1, an adenovirus that upregulates VDAC1 expression. In conclusion, this study is the first to demonstrate that vinegar/TMP pretreatment could induce myocardial protection of NPC due to downregulating VDAC1 expression, inhibiting oxidative stress, and preventing mitochondrial dysfunction; that is, VDAC1 is their target, and the mitochondria are their target organelles. TMP is one of the most important myocardial protective substances in vinegar.
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http://dx.doi.org/10.1155/2021/6670088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081599PMC
April 2021

Saponin surfactants used in drug delivery systems: A new application for natural medicine components.

Int J Pharm 2021 Jun 14;603:120709. Epub 2021 May 14.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China; Key Laboratory for Production Process Control and Quality Evaluation of Traditional Chinese Medicine, Beijing Municipal Science & Technology Commission, Beijing 102488, China. Electronic address:

Saponins are a group of compounds widely distributed in the plant kingdom. Due to their amphiphilic characteristic structure, saponins have high surface activity and self-assembly property and can be used as natural biosurfactants. Therefore, saponin has become a potential drug delivery system (DDS) carrier and has attracted the attention of many researchers. Increasing studies have found that when drugs combining with saponins, their solubility or bioavailability are improved. This phenomenon may be due to a synergistic mechanism and provides a potentially novel concept for DDS: saponins may be also used for carrier materials. This review emphasized the molecular characteristics and mechanism of saponins as carriers and the research on the morphology of saponin carriers. Besides, the article also introduced the role and application of saponins in DDS. Although there are still some limitations with the application of saponins such as cost, applicability, and hemolysis, the development of technology and in-depth molecular mechanism research will provide saponins with greater application prospects as DDS carriers.
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http://dx.doi.org/10.1016/j.ijpharm.2021.120709DOI Listing
June 2021

Hemodynamic testing using three-dimensional printing and computational fluid dynamics preoperatively may provide more information in mitral repair than traditional image dataset.

Ann Transl Med 2021 Apr;9(8):632

Department of Ultrasound Imaging, Renmin Hospital of Wuhan University, Wuhan, China.

Background: Mitral valve repair (MVR) has been considered superior to mitral replacement for degenerative MV disease and even rheumatic diseases. However, the repair rate varies widely depending on the medical center and the surgeons' experience. The aim of our study was to apply three-dimensional printing (3DP) and computational fluid dynamics (CFD) in surgical simulation to provide reference for surgical decision-making, especially for inexperienced surgeons.

Methods: Our study included retrospective and prospective cohorts. We first enrolled the retrospective cohort of 35 patients who were prepared to have MVR, aiming at exploring the feasibility of surgical simulation using 3DP and CFD. Three-dimensional transesophageal echocardiography (3D-TEE) and computed tomography angiography (CTA) were performed for all patients, and imaging data were fused to construct a 3D digital model. Next, the model was used to make the 3DP dynamic model and for CFD analysis. Mitral repair was simulated in both the 3DP dynamic model and CFD to predict surgical outcomes (grade of regurgitation and vena contracta width) and possible complications (systolic anterior motion, left ventricular outflow tract obstruction). Second, a prospective cohort of 20 patients was studied with 10 patients placed in a 3DP-guided group and 10 in an image-guided group. Rate of transformation to mitral replacement, surgery time, surgical outcomes, and surgical complications were compared between groups.

Results: Of the 35 patients retrospectively enrolled, 14 underwent MVR and 21 were transferred to mitral replacement. Surgical simulation for the 14 MVR patients showed high consistency with results. The result of surgical simulation for the 21 patients transferred to mitral replacement showed that 7 might have benefited from MVR. In the prospective cohort, the rate of transformation to mitral replacement and surgery time in the 3DP-guided group were significantly lower than those in the image-guided group.

Conclusions: 3DP and CFD models based on image data can be used for surgical simulation. These emerging technologies are now changing traditional models of diagnosis and treatment, and the role of imaging data will no longer be limited to diagnosis but will contribute more to assisting surgeons in choosing treatment strategies.
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http://dx.doi.org/10.21037/atm-20-7960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106081PMC
April 2021

Technology and Concept of Wastewater Treatment: Differences Between the Rhine Basin and the Yangtze Basin.

Bull Environ Contam Toxicol 2021 May 8. Epub 2021 May 8.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, 163 Xianlin Ave., Nanjing, 210023, China.

Based on field visits and literature research, the situations of several typical wastewater treatment plants (WWTPs) in the Rhine basin and the Yangtze basin were investigated, to compare the technology and concept of wastewater treatment in these two areas. Our results showed that WWTPs in the Rhine performed well in pollutant removal, and have shifted their focus to energy production and nutrient recovery; While in the Yangtze basin, most WWTPs still operate on the sole concept of pollution treatment. Though China's WWTPs attach importance to water reclamation, the related technologies are still under development. In years to come, the construction of New Concept WWTPs is expected by Chinese famous experts, to integrate sustainable wastewater treatment and energy/nutrient recovery. To better plan its future avenue in wastewater treatment, China is suggested to learn from the successful practice of energy production and nutrient recovery of WWTPs in the Rhine.
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http://dx.doi.org/10.1007/s00128-021-03217-4DOI Listing
May 2021

Clay mineral composition of upland soils and its implication for pedogenesis and soil taxonomy in subtropical China.

Sci Rep 2021 May 6;11(1):9707. Epub 2021 May 6.

College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, China.

Clay minerals are intermediate products generated during soil development, and their neoformation and transformation are closely related to pedogenesis. Here we aimed at identifying the difference in the clay mineral composition of upland soils derived from different parent materials and different soil-forming environments and exploring the importance of clay mineral composition in pedogenesis and soil taxonomy. We sampled 60 soil B horizons in Hunan Province of subtropical China by digging soils derived from granite (GR), slate and shale (SS), Quaternary red clay (QRC), limestone (LS), and sandstone (SDS). The clay mineral composition and its correlation with parent materials, elevation, micro-topography, and pedogenic processes were investigated using X-ray diffraction and Pearson's correlation analysis. The clay mineral was dominated by kaolinite, followed by 2:1-type minerals (illite and vermiculite), and a small fraction of mixed-layer minerals. The composition of soil clay minerals varied with parent materials. Kaolinite was predominant in soils derived from GR and LS; mixed-layer minerals prevailed in QRC, whereas illite and vermiculite were prevalent in SDS. In addition, elevation and micro-climate could also explain the variations in clay mineral composition. Increase in elevation was associated with decreased 1:1 clay mineral content and increased 2:1 clay mineral content, especially in soils developed from LS. The composition and content of clay minerals indicated that Ferrosols, Ultisols, and Acrisols had undergone intense weathering; Primosols, Entisols, and Leptosols were characterised by weak weathering, and Plinthic Ali-Udic Cambosols, Plinthudults, and Plinthosols were characterised by strong redox status. This study suggests that clay mineral composition is related to the parent material, climate, and micro-topography, and that it can serve as an indicator of pedogenesis and soil type in subtropical China.
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http://dx.doi.org/10.1038/s41598-021-89049-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102496PMC
May 2021

Inhibition mechanism of diacylated anthocyanins from purple sweet potato (Ipomoea batatas L.) against α-amylase and α-glucosidase.

Food Chem 2021 Oct 23;359:129934. Epub 2021 Apr 23.

Department of Pharmacy, Wuhan City Central Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China. Electronic address:

This study aimed to evaluate inhibitory activity of anthocyanins from purple sweet potato and blueberries against α-amylase and α-glucosidase, as well as investigate the inhibition mechanism of diacylated anthocyanins (Diacylated AF-PSP). Diacylated AF-PSP better inhibited α-amylase (IC = 0.078 mg mL) and α-glucosidase (IC = 1.56 mg mL) than other anthocyanin fractions, which was a mixed-type inhibitor. Fluorescence analysis indicated that Diacylated AF-PSP bound to the enzymes mainly through hydrogen bonds and influenced the microenvironments of proteins. Additionally, surface hydrophobicity and circular dichroism spectra results confirmed conformational changes in the enzymes induced by Diacylated AF-PSP. Molecular docking further demonstrated the interaction of Diacylated AF-PSP with enzyme active site, which might be stabilized by its acyl groups. Finally, 160 mg kg Diacylated AF-PSP significantly decreased (p < 0.01) blood glucose level peak by 20.52% after starch administration in SD rats. This study provided theoretical evidences for utilization of diacylated anthocyanins in hyperglycemia-management functional foods.
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http://dx.doi.org/10.1016/j.foodchem.2021.129934DOI Listing
October 2021

A New Group of Heterocyclic Nitrogenous Disinfection Byproducts (DBPs) in Drinking Water: Role of Extraction pH in Unknown DBP Exploration.

Environ Sci Technol 2021 May 30;55(10):6764-6772. Epub 2021 Apr 30.

State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.

pH adjustment prior to extraction is an important step in water sample pretreatment processes for exploration of new/unknown disinfection byproducts (DBPs) in drinking water. To achieve a better extraction efficiency, the pH of a water sample is usually adjusted to a low level (e.g., < 0.5) to ensure that target DBPs are in their neutral forms. However, such a practice may elude some amphoteric DBPs (especially those nitrogenous DBPs with multiple functional groups), which can accept protons at a low pH and lose protons at a high pH. In this study, with careful extraction pH selection and optimization, we first report the detection and identification of a new group of heterocyclic nitrogenous DBPs, halogenated pyridinols, in simulated drinking water using ultra performance liquid chromatography/electrospray ionization-triple quadrupole mass spectrometry and time-of-flight mass spectrometry, including 5-chloro-3-pyridinol, 2-bromo-3-pyridinol, 2,6-dichloro-4-pyridinol, 2,6-dibromo-3-pyridinol, 3-bromo-2-chloro-5-pyridinol, 5-bromo-2-chloro-3-pyridinol, 3,5,6-trichloro-2-pyridinol, and 2,4,6-tribromo-3-pyridinol. On the basis of the speciation of dissociated chemical species and recovery tests at different extraction pH values, it was found that, only at a pH of 3.0, all the eight new DBPs could achieve recoveries of >50%. With subsequent instrumental parameter optimization, the method detection and quantitation limits of the eight new DBPs were determined to be 0.04-1.58 and 0.15-4.11 ng/L, respectively. The optimized method enabled an accurate detection of the eight new DBPs in two real drinking water samples. Further aided with developmental and acute toxicity assays using zebrafish embryos, the developmental and acute toxicity of the new DBPs were found to be slightly lower than those of halogenated benzoquinones but dozens of times higher than those of commonly known DBPs such as tribromomethane and iodoacetic acid.
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http://dx.doi.org/10.1021/acs.est.1c00078DOI Listing
May 2021

Comparison of Osteogenic Differentiation Potential of Human Dental-Derived Stem Cells Isolated from Dental Pulp, Periodontal Ligament, Dental Follicle, and Alveolar Bone.

Stem Cells Int 2021 7;2021:6631905. Epub 2021 Apr 7.

Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, Liaoning, China.

Background: Mesenchymal stem cells (MSCs) have become promising candidates for regeneration medicine due to their multidifferentiation potential and immunomodulatory ability. Compared with classic MSCs derived from the bone marrow and fat, dental-derived MSCs show high plasticity, accessibility, and applicability. Therefore, they are considered alternative sources for regeneration medicine.

Methods: Four types of MSCs were isolated from the dental pulp, periodontal ligament, dental follicle, and alveolar bone of the same donor, and there were five different individuals. We analyzed their morphology, immunophenotype, proliferation rate, apoptosis, trilineage differentiation potential, and the gene expression during osteogenic differentiation.

Results: Our research demonstrated that DPSCs, PDLSCs, DFPCs and ABMMSCs exhibited similar morphology and immunophenotype. DFPCs showed a higher rate of proliferation and apoptosis. When cultured in the trilineage differentiation medium, all types of MSCs presented the differentiation potential of osteogenesis, adipogenesis, and chondrogenesis. Through staining and genetic analysis during osteogenic induction, ABMMSCs and PDLSCs showed the highest osteogenic ability, followed by DPSCs, and DFPCs were the lowest.

Conclusions: Overall, our results indicated that different dental-derived stem cells possessed different biological characteristics. For bone tissue engineering, ABMMSCs and PDLSCs can be used as optimal candidates of seed cells.
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http://dx.doi.org/10.1155/2021/6631905DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8049831PMC
April 2021

Caregivers' joint depressive symptoms and preschoolers' daily routines in Chinese three-generation families: Does household chaos matter?

Curr Psychol 2021 Apr 17:1-9. Epub 2021 Apr 17.

Department of Psychology, Faculty of Social Sciences, University of Macau, Macau, China.

The benefits of routines for children have been consistently demonstrated in previous literature. However, factors that may confer risks for child routines have seldom been examined, particularly in families where parents and grandparents co-care the children. This study aimed to investigate the associations of parents' and grandparents' depressive symptoms with preschoolers' daily routines in Chinese three-generation families and to determine whether household chaos mediated or moderated the associations. The participants were from 171 urban three-generation families where mothers, fathers, and grandmothers (97 paternal and 74 maternal) were primary caregivers. Mothers, fathers, and grandmothers reported their depressive symptoms at Wave 1; at Wave 2 (during the COVID-19 pandemic), caregivers reported household chaos and child routines. The results revealed that child routines were negatively predicted by parents' joint depressive symptoms rather than grandmothers' depressive symptoms. In the associations, household chaos acted as a mediator rather than a moderator. Specifically, household chaos marginally mediated the associations between parents' and grandmothers' depressive symptoms and child routines only in maternal three-generation families. These findings suggest that in three-generation families, caregivers with more depressive symptoms may elicit more chaotic family environments, which may in turn compromise their children's daily routines.
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http://dx.doi.org/10.1007/s12144-021-01595-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053031PMC
April 2021

Predictive Factors for In-Hospital Preoperative Rupture in Hyperacute Type A Aortic Dissection.

Heart Surg Forum 2021 04 23;24(2):E379-E386. Epub 2021 Apr 23.

Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing China.

Objective: This study aims to figure out risk factors of in-hospital preoperative rupture of hyperacute type A aortic dissection (haTAAD) patients and build a prediction and risk stratification model.

Methods: From January 2011 to December 2019, 830 patients diagnosed as haTAAD from Nanjing Drum Tower Hospital were enrolled. Among them, 799 patients received prompt surgery and 31 suffered aortic rupture before operation. The association between in-hospital preoperative rupture and perioperative parameters were examined. Best subset selection was used for feature selection and ROC curve was used to identify the model.

Results: Age, winter season, back pain, preoperative hypotension, albumin and globulin ratio, high serum phosphorus level are risk factors for in-hospital preoperative rupture of haTAAD. On the basis of six variables with AUC 0.828, a nomogram was established. According to the robustness test, actual in-hospital preoperative ruptures were fitted well.

Conclusions: The in-hospital rupture prediction model was developed using logistic regression analysis. High serum phosphorus level is one of the strongest predictors. This nomogram may be useful when evaluating the risk of aortic dissection in-hospital rupture in future trials.
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http://dx.doi.org/10.1532/hsf.3765DOI Listing
April 2021