Publications by authors named "Qing Ye"

744 Publications

External treatment of traditional Chinese medicine for myasthenia gravis: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Apr;100(15):e25475

Chengdu Eighth People's Hospital (Geriatric Hospital of Chengdu Medical College).

Background: Myasthenia gravis (MG) is an archetypal autoimmune disorder. The conventional treatments for this disease are drugs, plasma exchange, surgical, and so on. However, this disease is difficult to cure. A mass of studies revealed that the external treatment of traditional Chinese medicine (TCM) for MG is a safe and economical approach. The present study conducted a meta-analysis to compare TCM external treatment combined with modern medicine with modern medicine for MG, in order to determine which TCM external treatment intervention has the best relative efficacy, safety, and provide the best evidence for clinical practice.

Methods: PubMed, Cochrane Library, EMBASE, Web of Science, Springer, China National Knowledge Infrastructure (CNKI), Wan-fang database, VIP Chinese Science and Technique Journals Database, the Chinese Bio Medical Database (CBM), and Baidu Scholar were searched. The time of publication was limited from inception to February 28, 2021. Two reviewers independently searched for the selected articles and extract the data. The RevMan V.5.3 statistical software (Cochrane Collaboration) and Stata V.16.0 software were used to conduct the meta-analysis.

Results: The results of the systematic review and meta-analysis will be published in a peer-reviewed journal.

Conclusion: The present study provides a protocol that can be used in the systematic review and meta-analysis, with the intent to inform professionals on the external treatment of TCM for MG. These would lead to investigations on the use of the most external treatment of TCM for MG.

Trial Registration Number: INPLASY202110083.
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http://dx.doi.org/10.1097/MD.0000000000025475DOI Listing
April 2021

Self-reference Network-Related Interactions During the Process of Cognitive Impairment in the Early Stages of Alzheimer's Disease.

Front Aging Neurosci 2021 24;13:666437. Epub 2021 Mar 24.

Department of Neurology, Affiliated Drum Tower Hospital of Medical School and The State Key Laboratory of Pharmaceutical Biotechnology, Institute of Brain Science, Nanjing University, Nanjing, China.

: Normal establishment of cognition occurs after forming a sensation to stimuli from internal or external cues, in which self-reference processing may be partially involved. However, self-reference processing has been less studied in the Alzheimer's disease (AD) field within the self-reference network (SRN) and has instead been investigated within the default-mode network (DMN). Differences between these networks have been proven in the last decade, while ultra-early diagnoses have increased. Therefore, investigation of the altered pattern of SRN is significantly important, especially in the early stages of AD. : A total of 65 individuals, including 43 with mild cognitive impairment (MCI) and 22 cognitively normal individuals, participated in this study. The SRN, dorsal attention network (DAN), and salience network (SN) were constructed with resting-state functional magnetic resonance imaging (fMRI), and voxel-based analysis of variance (ANOVA) was used to explore significant regions of network interactions. Finally, the correlation between the network interactions and clinical characteristics was analyzed. : We discovered four interactions among the three networks, with the SRN showing different distributions in the left and right hemispheres from the DAN and SN and modulated interactions between them. Group differences in the interactions that were impaired in MCI patients indicated that the degree of damage was most severe in the SRN, least severe in the SN, and intermediate in the DAN. The two SRN-related interactions showed positive effects on the executive and memory performances of MCI patients with no overlap with the clinical assessments performed in this study. : This study is the first and primary evidence of SRN interactions related to MCI patients' functional performance. The influence of the SRN in the ultra-early stages of AD is nonnegligible. There are still many unknowns regarding the contribution of the SRN in AD progression, and we strongly recommend future research in this area.
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http://dx.doi.org/10.3389/fnagi.2021.666437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024683PMC
March 2021

Identification of active molecules against Mycobacterium tuberculosis through machine learning.

Brief Bioinform 2021 Apr 5. Epub 2021 Apr 5.

College of Pharmaceutical Sciences at Zhejiang University, China.

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb) and it has been one of the top 10 causes of death globally. Drug-resistant tuberculosis (XDR-TB), extensively resistant to the commonly used first-line drugs, has emerged as a major challenge to TB treatment. Hence, it is quite necessary to discover novel drug candidates for TB treatment. In this study, based on different types of molecular representations, four machine learning (ML) algorithms, including support vector machine, random forest (RF), extreme gradient boosting (XGBoost) and deep neural networks (DNN), were used to develop classification models to distinguish Mtb inhibitors from noninhibitors. The results demonstrate that the XGBoost model exhibits the best prediction performance. Then, two consensus strategies were employed to integrate the predictions from multiple models. The evaluation results illustrate that the consensus model by stacking the RF, XGBoost and DNN predictions offers the best predictions with area under the receiver operating characteristic curve of 0.842 and 0.942 for the 10-fold cross-validated training set and external test set, respectively. Besides, the association between the important descriptors and the bioactivities of molecules was interpreted by using the Shapley additive explanations method. Finally, an online webserver called ChemTB (http://cadd.zju.edu.cn/chemtb/) was developed, and it offers a freely available computational tool to detect potential Mtb inhibitors.
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http://dx.doi.org/10.1093/bib/bbab068DOI Listing
April 2021

Three-dimensional remodeling of functional cerebrovascular architecture and gliovascular unit in leptin receptor-deficient mice.

J Cereb Blood Flow Metab 2021 Apr 4:271678X211006596. Epub 2021 Apr 4.

Veterans Affairs Pittsburgh Healthcare System, Pittsburgh, PA, USA.

The cerebrovascular sequelae of diabetes render victims more susceptible to ischemic stroke, vascular cognitive impairment, and Alzheimer's disease. However, limited knowledge exists on the progressive changes in cerebrovascular structure and functional remodeling in type 2 diabetes. To ascertain the impact of diabetes on whole-brain cerebrovascular perfusion, leptin-receptor-deficient mice were transcardially injected with tomato-lectin before sacrifice. The whole brain was clarified by the Fast free-of-acrylamide clearing tissue technique. Functional vascular anatomy of the cerebrum was visualized by light-sheet microscopy, followed by analysis in Imaris software. We observed enhanced neovascularization in adult db/db mice, characterized by increased branch level and loop structures. Microvascular hypoperfusion was initially detected in juvenile db/db mice, suggesting early onset of insufficient microcirculation. Furthermore, gliovascular unit remodeling was verified by loss of pericytes and overactivation of microglia and astrocytes in adult diabetic mice. However, the integrity of the blood-brain barrier (BBB) was fundamentally preserved, as shown by a lack of extravasation of IgG into the brain parenchyma. In summary, we, for the first time, reveal that functional cerebrovascular remodeling occurs as early as four weeks in db/db mice and the deficit in gliovascular coupling may play a role in cerebral hypoperfusion before BBB breakdown in 16-week-old db/db mice.
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http://dx.doi.org/10.1177/0271678X211006596DOI Listing
April 2021

Distributed optical fiber hydrophone based on Φ-OTDR and its field test.

Opt Express 2021 Feb;29(3):3147-3162

In this letter, a distributed optical fiber hydrophone (DOFH) based on Φ-OTDR is demonstrated and tested in the field. The specially designed sensitized optical cable with sensitivity up to -146 dB rad/µPa/m is introduced, and an array signal processing model for DOFH is constructed to analyze the equivalence and specificity of the distributed array of acoustic sensors. In the field test, a 104-meter-long optical cable and a Φ-OTDR system based on heterodyne coherent detection (Het Φ-OTDR) is utilized, and underwater acoustic signal spatial spectrum estimation, beamforming and motion trajectory tracking with high accuracy can be realized. As far as we know, this is the first report on the field trial of DOFH based on Φ-OTDR. The DOFH has the potential to achieve an array range of tens of kilometers, with elements spaced up to the meter level and flexible configuration, which has a broad application prospect for marine acoustic detection.
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http://dx.doi.org/10.1364/OE.414598DOI Listing
February 2021

UCHL1 inhibition attenuates cardiac fibrosis via modulation of nuclear factor-κB signaling in fibroblasts.

Eur J Pharmacol 2021 Mar 19;900:174045. Epub 2021 Mar 19.

Provincial Hospital of Anhui Medical University, Hefei, 230000, Anhui, PR China; The First Hospital of University of Science and Technology of China, Hefei, 230000, Anhui, PR China. Electronic address:

The ubiquitin-proteasome system (UPS) plays an essential role in cellular homeostasis and myocardial function. Ubiquitin carboxy-terminal hydrolase 1 (UCHL1) is involved in cardiac remodeling, but its underlying mechanisms are largely unknown. Here, we observed that the UCHL1 was significantly up-regulated in angiotensin II-infused heart and primary cardiac fibroblast (CF). Systemic administration of the UCHL1 inhibitor LDN57444 significantly ameliorated cardiac fibrosis and improved cardiac function induced by angiotensin II. Also, LDN57444 inhibited CF cell proliferation as well as attenuated collagen I, and CTGF gene expression in the presence of Ang II. Mechanistically, UCHL1 promotes angiotensin II-induced fibrotic responses by way of activating nuclear factor kappa B (NF-κB) signaling. Moreover, suppression of the NF-κB pathway interfered with UCHL1 overexpression-mediated fibrotic responses. Besides, the chromatin immunoprecipitation assay demonstrated that NF-κB can bind to the UCHL1 promoter and trigger its transcription in cardiac fibroblasts. These findings suggest that UCHL1 positively regulates cardiac fibrosis by modulating NF-κB signaling pathway and identify UCHL1 could be a new treatment strategy for cardiac fibrosis.
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http://dx.doi.org/10.1016/j.ejphar.2021.174045DOI Listing
March 2021

Low-temperature (NO + O) adsorption performance of alkaline earth metal-doped C-FDU-15.

J Environ Sci (China) 2021 May 16;103:172-184. Epub 2020 Nov 16.

Beijing Key Laboratory for Green Catalysis and Separation, Key Laboratory of Beijing on Regional Air Pollution Control, Key Laboratory of Advanced Functional Materials, Education Ministry of China, and Laboratory of Catalysis Chemistry and Nanoscience, Department of Chemistry and Chemical Engineering, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address:

To improve the removal capacity of NO + O effectively, the alkaline earth metal-doped order mesoporous carbon (A-C-FDU-15(0.001) (A = Mg, Ca, Sr and Ba)) and Mg-C-FDU-15(x) (x = 0.001-0.003) samples were prepared, and their physicochemical and NO + O adsorption properties were determined by means of various techniques. The results show that the sequence in (NO + O) adsorption performance was as follows: Mg-C-FDU-15(0.001) (93.2 mg/g) > Ca-C-FDU-15(0.001) (82.2 mg/g) > Sr-C-FDU-15(0.001) (76.1 mg/g) > Ba-C-FDU-15(0.001) (72.9 mg/g) > C-FDU-15 (67.1 mg/g). Among all of the A-C-FDU-15(0.001) samples, Mg-C-FDU-15(0.001) possessed the highest (NO + O) adsorption capacity (106.2 mg/g). The species of alkaline earth metals and basic sites were important factors determining the adsorption of NO + O on the A-C-FDU-15(x) samples, and (NO + O) adsorption on the samples was mainly chemical adsorption. Combined with the results of (NO + O)-temperature-programmed desorption ((NO + O)-TPD) and in situ diffused reflectance infrared Fourier transform spectroscopy (DRIFTS) characterization, we deduced that there were two main pathways of (NO + O) adsorption: one was first the conversion of NO and O to NO and then part of NO was converted to NO and NO; and the other was the direct oxidation of NO to NO and NO.
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http://dx.doi.org/10.1016/j.jes.2020.10.014DOI Listing
May 2021

Leaf hydraulic acclimation to nitrogen addition of two dominant tree species in a subtropical forest.

Sci Total Environ 2021 Jun 27;771:145415. Epub 2021 Jan 27.

College of Environment and Planning, Henan University, Jinming Avenue, Kaifeng 475004, China.

Plant hydraulic traits have been shown to be sensitive to changes in nitrogen (N) availability in short-term studies largely using seedlings or saplings. The extent and the magnitude of N-sensitivity of the field grown mature trees in long-term experiments, however, are relatively unknown. Here, we investigated responses of leaf water relations and morphological and anatomical traits of two dominant tree species (Castanopsis chinensis and Schima superba) to a six-year canopy N addition in a subtropical forest. We found that N addition increased leaf hydraulic conductivity in both species along with higher transpiration rate and less negative water potential at 50% loss of leaf hydraulic conductivity and at leaf turgor loss point. Examination of leaf morphological and anatomical traits revealed that increased leaf hydraulic efficiency was at least in part due to increased vessel diameter which also compromised the hydraulic safety under increased water stress. Moreover, reduced vessel reinforcement and increased thickness shrinkage index further interpreted the increases in leaf hydraulic vulnerability under N addition. Our results demonstrated that N deposition may lead to increases of plant water loss to the atmosphere as well as tree vulnerability to drought.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145415DOI Listing
June 2021

Exosomal MicroRNAs Contribute to Cognitive Impairment in Hypertensive Patients by Decreasing Frontal Cerebrovascular Reactivity.

Front Neurosci 2021 1;15:614220. Epub 2021 Mar 1.

The State Key Laboratory of Pharmaceutical Biotechnology, Department of Neurology, Medical School, Drum Tower Hospital, Institute of Brain Science, Nanjing University, Nanjing, China.

Mechanisms underlying cognitive impairment (CI) in hypertensive patients remain relatively unclear. The present study aimed to explore the relationship among serum exosomal microRNAs (miRNAs), cerebrovascular reactivity (CVR), and cognitive function in hypertensive patients. Seventy-three hypertensive patients with CI (HT-CI), 67 hypertensive patients with normal cognition (HT-NC), and 37 healthy controls underwent identification of exosomal miRNA, multimodal magnetic resonance imaging (MRI) scans, and neuropsychological tests. CVR mapping was investigated based on resting-state functional MRI data. Compared with healthy subjects and HT-NC subjects, HT-CI subjects displayed decreased serum exosomal miRNA-330-3p. The group difference of CVR was mainly found in the left frontal lobe and demonstrated that HT-CI group had a lower CVR than both HT-NC group and control group. Furthermore, both the CVR in the left medial superior frontal gyrus and the miRNA-330-3p level were significantly correlated with executive function ( = -0.275, = 0.021, and = -0.246, = 0.04, respectively) in HT-CI subjects, and the CVR was significantly correlated with the miRNA-330-3p level ( = 0.246, = 0.040). Notably, path analysis showed that the CVR mediated the association between miRNA-330-3p and executive function. In conclusion, decreased miRNA-330-3p might contribute to CI in hypertensive patients by decreasing frontal CVR and could be a biomarker of early diagnosis.
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http://dx.doi.org/10.3389/fnins.2021.614220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957933PMC
March 2021

Joint association of cigarette smoking and PM with COPD among urban and rural adults in regional China.

BMC Pulm Med 2021 Mar 15;21(1):87. Epub 2021 Mar 15.

Nanjing Municipal Center for Disease Control and Prevention, 3, Zizhulin, Nanjing, 210037, China.

Background: Cigarette smoking and PM are important risk factors of Chronic Obstructive Pulmonary Disease (COPD). However, the joint association of cigarette smoking and PM with COPD is unknown.

Methods: A community-based study was conducted among urban and rural adults aged 40 + years between May and December of 2015 in Jiangsu Province, China. The outcome variable was spirometry-defined COPD. Explanatory measures were smoking status (non-smokers or smokers) and PM exposure [low level (< 75 μg/m) or high level (≥ 75 μg/m)]. Mixed-effects logistic regression models were applied to calculate the odds ratio (OR) and 95% confidence interval (CI) to investigate the associations of cigarette smoking and PM with COPD.

Results: The prevalence of COPD was 11.9% (95% CI = 10.9%, 13.0%) within the overall 3407 participants in this study. After adjustment for potential confounders and community-level clustering effect, smokers tended to develop COPD relative to non-smokers (OR = 2.46, 95% CI 1.76, 3.43), while only smokers exposed to high level PM were more likely to experience COPD (OR = 1.36; 95% CI 1.01, 1.83) compared to their counterparts exposed to low level PM. Meanwhile, compared to non-smokers who exposed to low level PM, non-smokers who exposed to high level PM (OR = 1.10, 95% CI 0.74, 1.64), smokers who exposed to low (OR = 2.22, 95% CI 1.51, 3.27) or high level PM (OR = 3.14, 95% CI 2.15, 4.59) were, respectively, more like to develop COPD.

Conclusions: Cigarette smoking was positively associated with COPD among overall participants, while PM was in positive relation to COPD among smokers only. Moreover, cigarette smoking and PM might have an additive effect on the risk of COPD among adult smokers aged 40 years or older in China.
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http://dx.doi.org/10.1186/s12890-021-01465-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962238PMC
March 2021

Sequential macrophage transition facilitates endogenous bone regeneration induced by Zn-doped porous microcrystalline bioactive glass.

J Mater Chem B 2021 Mar 15;9(12):2885-2898. Epub 2021 Mar 15.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

Macrophages play an important role in the immune microenvironment during bone healing, and sequential macrophage phenotypic transition could achieve superior osteogenic outcomes. Microcrystalline bioactive glasses (MCBGs) with osteoimmunomodulatory effects show potential in bone tissue regeneration. Zinc (Zn) has been approved to coordinate innate and adaptive immunity. Therefore, in this study, different amounts of ZnO were incorporated into microcrystalline bioactive glass to improve its immunomodulatory ability. The effect of Zn-MCBG ionic extracts on macrophage transition was studied, and the 5Zn-MCBG extracts could orchestrate sequential M1-to-M2 macrophage transition and promote the expression of proinflammatory and anti-inflammatory genes and cytokine expression to induce human bone marrow stromal cells (hBMSCs) osteogenic differentiation in vitro. Macroporous Zn-MCBG scaffolds containing mesopores were fabricated and showed good cell adhesion and feasible apatite formation when immersed in SBF in vitro. Furthermore, a rat calvarial defect model was used to confirm that the Zn-MCBG scaffold could modulate macrophage phenotypic transition and create a desirable osteogenic microenvironment to promote osteogenesis in vivo.
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http://dx.doi.org/10.1039/d0tb02884cDOI Listing
March 2021

Potential false-positive reasons for SARS-CoV-2 antibody testing and its solution.

J Med Virol 2021 Mar 12. Epub 2021 Mar 12.

Laboratory Medicine Department, College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou, China.

Coronavirus disease 2019 (COVID-19) has brought a huge impact on global health and the economy. Early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is essential for epidemic prevention and control. The detection of SARS-CoV-2 antibodies is an important criterion for diagnosing COVID-19. However, SARS-CoV-2 antibody testing also has certain false positives causing confusion in clinical diagnosis. This article summarizes the causes of false-positive detection of SARS-CoV-2 antibodies in clinical practice. The results indicate that the most common endogenous interferences include rheumatoid factor, heterophile antibodies, human anti-animal antibodies, lysozyme, complement, and cross-antigens. The exogenous interference is mainly incomplete coagulation of the specimen, contamination of the specimen, and insufficient optimization of the diagnostic kit's reaction system.
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http://dx.doi.org/10.1002/jmv.26937DOI Listing
March 2021

Typing FGFR2 translocation determines the response to targeted therapy of intrahepatic cholangiocarcinomas.

Cell Death Dis 2021 Mar 11;12(3):256. Epub 2021 Mar 11.

Department of Medical Genetics, Nanjing Medical University, Nanjing, 211166, Jiangsu Province, China.

Chromosomal translocations involving fibroblast growth factor receptor 2 (FGFR2) gene at the breakpoints are common genetic lesions in intrahepatic cholangiocarcinoma (ICC) and the resultant fusion protein products have emerged as promising druggable targets. However, predicting the sensitivity of FGFR2 fusions to FGFR kinase inhibitors is crucial to the prognosis of the ICC-targeted therapy. Here, we report identification of nine FGFR2 translocations out of 173 (5.2%) ICC tumors. Although clinicopathologically these FGFR2 translocation bearing ICC tumors are indistinguishable from the rest of the cohort, they are invariably of the mass-forming type originated from the small bile duct. We show that the protein products of FGFR2 fusions can be classified into three subtypes based on the breaking positions of the fusion partners: the classical fusions that retain the tyrosine kinase (TK) and the Immunoglobulin (Ig)-like domains (n = 6); the sub-classical fusions that retain only the TK domain without the Ig-like domain (n = 1); and the non-classical fusions that lack both the TK and Ig-like domains (n = 2). We demonstrate that cholangiocarcinoma cells engineered to express the classical and sub-classical fusions show sensitivity to FGFR-specific kinase inhibitors as evident by the suppression of MAPK/ERK and AKT/PI3K activities following the inhibitor treatment. Furthermore, the kinase-deficient mutant of the sub-classical fusion also lost its sensitivity to the FGFR-specific inhibitors. Taken together, our study suggests that it is essential to determine the breakpoint and type of FGFR2 fusions in the small bile duct subtype of ICC for the targeted treatment.
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http://dx.doi.org/10.1038/s41419-021-03548-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946919PMC
March 2021

Dither removing Fourier ptychographic microscope based on a two-axis rotation stage.

J Biomed Opt 2021 Mar;26(3)

Ministry of Education, Nankai University, School of Physics, Key Laboratory of Weak-Light Nonlinear, China.

Significance: Large space-bandwidth product is highly desirable in many biomedical imaging. Fourier ptychographic microscopy (FPM) is a computational imaging technique that can significantly increase the space-bandwidth product of a standard microscope. The illuminator of a Fourier ptychographic microscope is not flexible at present, and it is inconvenient to meet different imaging needs.

Aim: An illuminator based on a two-axis motorized rotation stage was presented to provide a more flexible illuminating way with the goal of meeting different imaging needs.

Approach: The illuminator adopts a concentric illuminating method to provide coherent illumination in any direction on the sample plane. The sampling pattern can be freely designed and changed according to the parameters of the imaging system. A dither removing algorithm was proposed to remove the potential dither influence introduced in the image acquisition process.

Results: The illuminator could be conveniently integrated into different imaging systems. The feasibility and flexibility were demonstrated by applying it to imaging systems with numerical aperture of 0.045 and 0.01. The resolution gain is about 4- and 13-fold, respectively. The effectiveness of the dither removing algorithm was validated in both simulation and experiment.

Conclusions: A more flexible illuminator for FPM was presented to meet different imaging needs. A dither removing algorithm was proposed to remove dither influence.
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http://dx.doi.org/10.1117/1.JBO.26.3.036501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930810PMC
March 2021

Roxadustat for Renal Anemia in ESRD from PKD Patients: Is It Safe Enough?

J Am Soc Nephrol 2021 Mar 1. Epub 2021 Mar 1.

Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China

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http://dx.doi.org/10.1681/ASN.2020111664DOI Listing
March 2021

Inhibiting microRNA-155 attenuates atrial fibrillation by targeting CACNA1C.

J Mol Cell Cardiol 2021 Feb 24;155:58-65. Epub 2021 Feb 24.

Key Laboratory of Interdisciplinary Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; Medical School of University of Chinese Academy of Sciences, Beijing, China; Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China. Electronic address:

Background: Reduction in L-type Ca current (I) density is a hallmark of the electrical remodeling in atrial fibrillation (AF). The expression of miR-155, whose predicted target gene is the α1c subunit of the calcium channel (CACNA1C), was upregulated in atrial cardiomyocytes (aCMs) from patients with paroxysmal AF.The study is to determine miR-155 could target the gene expression of I and contribute to electrical remodeling in AF.

Methods: The expression of miR-155 and CACNA1C was assessed in aCMs from patients with paroxysmal AF and healthy control. I properties were observed after miR-155 transfection in human induced pluripotent stem cell derived atrial cardiomyocytes (hiPSC-aCMs). Furthermore, an miR-155 transgene (Tg) and knock-out (KO) mouse model was generated to determine whether miR-155 was involved in I-related electrical remodeling in AF by targeting CACNA1C.

Results: The expression level of miR-155 was increased, while the expression level of CACNA1C reduced in the aCMs of patients with AF. miR-155 transfection in hiPSC-aCMs produced changes in I properties qualitatively similar to those produced by AF. miR-155/Tg mice developed a shortened action potential duration and increased vulnerability to AF, which was associated with decreased I and attenuated by an miR-155 inhibitor. Finally, the genetic inhibition of miR-155 prevented AF induction in miR-155/KO mice with no changes in I properties.

Conclusions: The increased miR-155 expression in aCMs was sufficient for the reduction in the density of I and the underlying electronic remodeling. The inhibition of miR-155 prevented I-related electric remodeling in AF and might constitute a novel anti-AF approach targeting electrical remodeling.
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http://dx.doi.org/10.1016/j.yjmcc.2021.02.008DOI Listing
February 2021

L. Extract Protects against Lipopolysaccharide-Induced Inflammation in RAW 264.7 Macrophage and Zebrafish via Inhibition of Akt/MAPKs and NF-B/iNOS-NO Signaling Pathways.

Evid Based Complement Alternat Med 2021 12;2021:6628561. Epub 2021 Feb 12.

Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Inflammation response is a regulated cellular process and excessive inflammation has been recognized in numerous diseases, such as cardiovascular disease, neurodegenerative disease, inflammatory bowel disease, and cancer. L. (TT), also known as Bai Jili in Chinese, has been applied in traditional Chinese medicine for thousands of years while its anti-inflammatory activity and underlying mechanism are not fully elucidated. Here, we hypothesize L. extract (BJL) which presents anti-inflammatory effect, and the action mechanism was also investigated. We employed the transgenic zebrafish line Tg(MPO:GFP), which expresses green fluorescence protein (GFP) in neutrophils, and mice macrophage RAW 264.7 cells as the and model to evaluate the anti-inflammatory effect of BJL, respectively. The production of nitric oxide (NO) was measured by Griess reagent. The mRNA expression levels of inflammatory cytokines and inducible nitric oxide synthase (iNOS) were measured by real-time PCR, and the intracellular total or phosphorylated protein levels of NF-B, Akt, and MAPKs including MEK, ERK, p38, and JNK were detected by western blot. We found that BJL significantly inhibited fin transection or lipopolysaccharide- (LPS-) induced neutrophil migration and aggregation in zebrafish . In mice macrophage RAW 264.7 cells, BJL ameliorated LPS-triggered excessive release of NO and transcription of inflammatory cytokine genes including tumor necrosis factor-alpha (TNF-), interleukin-6 (IL-6), and interleukin-1 beta (IL-1). BJL also reduced the LPS-induced elevations of intracellular iNOS and nuclear factor kappa B (NF-B) which mediate the cellular NO and inflammatory cytokine productions, respectively. Moreover, LPS dramatically increased the phosphorylation of Akt and MAPKs including MEK, ERK, p38, and JNK in RAW 264.7 cells, while cotreatment BJL with LPS suppressed their phosphorylation. Taken together, our data suggested that BJL presented potent anti-inflammatory effect and the underlying mechanism was closely related to the inhibition of Akt/MAPKs and NF-B/iNOS-NO signaling pathways.
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http://dx.doi.org/10.1155/2021/6628561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895590PMC
February 2021

Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1.

Acta Physiol (Oxf) 2021 Feb 22:e13634. Epub 2021 Feb 22.

Department of Nephrology, The Children Hospital of Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China.

Aims: Diabetic nephropathy (DN) is a leading cause of end-stage renal disease. BASP1 (brain acid-soluble protein) is up-regulated in podocyte-specific protein phosphatase 2A knockout mice (Pod-PP2A-KO) that develop kidney dysfunction. Here, we explore the role of BASP1 for podocytes in DN.

Methods: BASP1 was assessed in kidneys from DN patients and DN mouse models, podocyte specific BASP1 knockout mice (Pod-BASP1-KO mice) were generated and studied in vivo. Furthermore, podocyte injury and apoptosis were measured after BASP1 knockdown and overexpression in a mouse podocyte cell line (MPC5). Potential signalling pathways involved in podocyte apoptosis were detected.

Results: BASP1 expression was up-regulated in DN patients compared to normal controls. BASP1 specific deletion in podocytes protected against podocyte injury by reducing the loss of expression of slit diaphragm molecules and foot process effacement in the DN model. BASP1 promoted actin cytoskeleton rearrangements and apoptosis in the MPC5 podocyte line. Molecules involved in the p53 pathway were down-regulated in BASP1 knockdown podocytes treated with high glucose compared to controls. BASP1 promoted podocyte apoptosis and P53 pathway activation through co-repression with Wilms' tumour 1 transcription factor (WT1).

Conclusion: BASP1 activates the p53 pathway through modulation of WT1 to induce podocyte apoptosis in diabetic nephropathy.
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http://dx.doi.org/10.1111/apha.13634DOI Listing
February 2021

Impaired Cellular Immunity to SARS-CoV-2 in Severe COVID-19 Patients.

Front Immunol 2021 2;12:603563. Epub 2021 Feb 2.

Institute for Immunology and School of Medicine, Tsinghua University, Beijing, China.

The high infection rate and rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) make it a world-wide pandemic. Individuals infected by the virus exhibited different degrees of symptoms, and most convalescent individuals have been shown to develop both cellular and humoral immune responses. However, virus-specific adaptive immune responses in severe patients during acute phase have not been thoroughly studied. Here, we found that in a group of COVID-19 patients with acute respiratory distress syndrome (ARDS) during hospitalization, most of them mounted SARS-CoV-2-specific antibody responses, including neutralizing antibodies. However, compared to healthy controls, the percentages and absolute numbers of both NK cells and CD8 T cells were significantly reduced, with decreased IFNγ expression in CD4 T cells in peripheral blood from severe patients. Most notably, their peripheral blood lymphocytes failed in producing IFNγ against viral proteins. Thus, severe COVID-19 patients at acute infection stage developed SARS-CoV-2-specific antibody responses but were impaired in cellular immunity, which emphasizes on the role of cellular immunity in COVID-19.
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http://dx.doi.org/10.3389/fimmu.2021.603563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884325PMC
February 2021

Efficacy and safety of anticoagulants in liver cirrhosis patients with portal vein thrombosis: A meta-analysis.

Clin Res Hepatol Gastroenterol 2021 Feb 15:101649. Epub 2021 Feb 15.

Department of gastroenterology, Tianjin Third Central Hospital, Tianjin, 300170, China. Electronic address:

Objective: To investigate the efficacy and safety of anticoagulants in liver cirrhosis patients with portal vein thrombosis (PVT).

Methods: PubMed, BioMed Central, Cochrane Library and Web of Science were retrieved to identify relevant literature. Forest plots were applied to display the results of the meta-analysis. The odds ratios (ORs) were used as the effect index for the enumeration data, and the effect size was expressed as 95% confidence intervals (CIs). Publication bias was evaluated by funnel plots and Egger's test.

Results: Eight articles included 225 patients with liver cirrhosis and PVT receiving anticoagulants and 232 not receiving anticoagulants. The data demonstrated that the recanalization rate of PVT was significantly higher in patients with anticoagulant treatment than in patients without anticoagulant treatment (OR=5.60; 95% CI: 3.40-9.22; P<0.001). The exacerbation risk of PVT was significantly lower in patients with anticoagulant treatment than in patients without anticoagulant treatment (OR=0.15; 95% CI: 0.04-0.54; P<0.001). A significantly lower portal hypertension bleeding effect was observed in patients with anticoagulant treatment than in patients without anticoagulant treatment (OR=0.21; 95% CI: 0.10-0.45; P<0.001). Low molecular weight heparins (LMWH) were more effective in preventing the PVT exacerbation in liver cirrhosis patients with PVT than warfarin (OR=0.16; 95% CI: 0.08-0.35).

Conclusions: Anticoagulants were effective and safe in treating patients with liver cirrhosis and PVT as they could increase the PVT recanalization rate and decrease the risks of PVT exacerbation and portal hypertension bleeding.
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http://dx.doi.org/10.1016/j.clinre.2021.101649DOI Listing
February 2021

Diagnosis of COVID-19 Pneumonia Based on Graph Convolutional Network.

Front Med (Lausanne) 2020 21;7:612962. Epub 2021 Jan 21.

School of Innovation and Entrepreneurship, Nanjing Institute of Technology, Nanjing, China.

A three-dimensional (3D) deep learning method is proposed, which enables the rapid diagnosis of coronavirus disease 2019 (COVID-19) and thus significantly reduces the burden on radiologists and physicians. Inspired by the fact that the current chest computed tomography (CT) datasets are diversified in equipment types, we propose a COVID-19 graph in a graph convolutional network (GCN) to incorporate multiple datasets that differentiate the COVID-19 infected cases from normal controls. Specifically, we first apply a 3D convolutional neural network (3D-CNN) to extract image features from the initial 3D-CT images. In this part, a transfer learning method is proposed to improve the performance, which uses the task of predicting equipment type to initialize the parameters of the 3D-CNN structure. Second, we design a COVID-19 graph in GCN based on the extracted features. The graph divides all samples into several clusters, and samples with the same equipment type compose a cluster. Then we establish edge connections between samples in the same cluster. To compute accurate edge weights, we propose to combine the correlation distance of the extracted features and the score differences of subjects from the 3D-CNN structure. Lastly, by inputting the COVID-19 graph into GCN, we obtain the final diagnosis results. In experiments, the dataset contains 399 COVID-19 infected cases, and 400 normal controls from six equipment types. Experimental results show that the accuracy, sensitivity, and specificity of our method reach 98.5%, 99.9%, and 97%, respectively.
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http://dx.doi.org/10.3389/fmed.2020.612962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875085PMC
January 2021

Acupuncture for diabetic neurogenic bladder: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Feb;100(6):e24573

Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, China.

Background: Diabetic neurogenic bladder (DNB) is one of the common complications of diabetes mellitus, which has a high prevalence rate. Some research suggested that acupuncture can improve the clinical symptoms of diabetic neurogenic bladder patients, but there is no systematic review or meta-analysis to assess this therapy. Therefore, this study aims to explore the effectiveness and safety of acupuncture for patients with DNB.

Methods: In this study, we will search for electronic databases including the Cochrane Library, Web of Science, PubMed, MEDLINE, EMBASE,China National Knowledge Infrastructure (CNKI), Wan-Fang, and Baidu Scholar Database from inception to December 2020. We will select randomized controlled trials that have been published in English or Chinese related to acupuncture for DNB. Selection of study, extraction of data, and assessment of study quality will be performed independently by 2 researchers, and we will use Revman 5.3 software which is provided by Cochrane assistance network, to perform the data analysis.

Results: This study will provide evidence of the effectiveness and safety of acupuncture for DNB.

Conclusion: This study will clarify whether acupuncture is an effective treatment for DNB, and will also provide a reference for clinical practice and guidelines development.
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http://dx.doi.org/10.1097/MD.0000000000024573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886445PMC
February 2021

A clinical characteristic analysis of five cases of rare bilateral cerebral peduncular infarction (BCPI) with the 'Mickey Mouse ears' sign.

Authors:
Qing Ye Tao Xiang

Brain Inj 2021 Feb 9:1-5. Epub 2021 Feb 9.

Department of Neurology, The First Affiliated Hospital of University of South China , Hengyang, Hunan, China.

: To investigate the clinical manifestations, magnetic resonance imaging (MRI) features, and prognosis of patients diagnosed with bilateral cerebral peduncular infarction (BCPI) with the 'Mickey Mouse ears' sign. : This study, retrospectively summarized the clinical features, MRI and magnetic resonance angiography (MRA) manifestations, and prognosis of 5 patients diagnosed with BCPI and with the 'Mickey Mouse ears' sign from our hospital. In the meantime, we also systematically analyzed and summarized the clinical and imaging features, and prognosis in combination with relevant cases from the national and international literature that had been reported. : In our study, five cases presented tetraplegia whereas 4 cases presented disturbance of consciousness. In all cases, the 'Mickey Mouse ears' sign was observed in the mid-brain level using a diffusion-weighted imaging (DWI) cross-section. The MRA images showed thrombosis on either the basilar artery (BA), posterior cerebral artery (PCA), or superior cerebellar artery (SCA). All the studied patients received standardized treatment for cerebral infarction. However, three patients died and the remaining two were left in a locked-in syndrome state. : BCPI is a very rare stroke disease and its main clinical manifestations are locked-in syndrome and persistent vegetative states. However, the obtained imaging features on the brain-MRI of patients diagnosed with BCPI with the 'Mickey Mouse ears' sign can predict a bad prognosis.
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http://dx.doi.org/10.1080/02699052.2021.1872097DOI Listing
February 2021

Role of iodine oxoacids in atmospheric aerosol nucleation.

Authors:
Xu-Cheng He Yee Jun Tham Lubna Dada Mingyi Wang Henning Finkenzeller Dominik Stolzenburg Siddharth Iyer Mario Simon Andreas Kürten Jiali Shen Birte Rörup Matti Rissanen Siegfried Schobesberger Rima Baalbaki Dongyu S Wang Theodore K Koenig Tuija Jokinen Nina Sarnela Lisa J Beck João Almeida Stavros Amanatidis António Amorim Farnoush Ataei Andrea Baccarini Barbara Bertozzi Federico Bianchi Sophia Brilke Lucía Caudillo Dexian Chen Randall Chiu Biwu Chu António Dias Aijun Ding Josef Dommen Jonathan Duplissy Imad El Haddad Loïc Gonzalez Carracedo Manuel Granzin Armin Hansel Martin Heinritzi Victoria Hofbauer Heikki Junninen Juha Kangasluoma Deniz Kemppainen Changhyuk Kim Weimeng Kong Jordan E Krechmer Aleksander Kvashin Totti Laitinen Houssni Lamkaddam Chuan Ping Lee Katrianne Lehtipalo Markus Leiminger Zijun Li Vladimir Makhmutov Hanna E Manninen Guillaume Marie Ruby Marten Serge Mathot Roy L Mauldin Bernhard Mentler Ottmar Möhler Tatjana Müller Wei Nie Antti Onnela Tuukka Petäjä Joschka Pfeifer Maxim Philippov Ananth Ranjithkumar Alfonso Saiz-Lopez Imre Salma Wiebke Scholz Simone Schuchmann Benjamin Schulze Gerhard Steiner Yuri Stozhkov Christian Tauber António Tomé Roseline C Thakur Olli Väisänen Miguel Vazquez-Pufleau Andrea C Wagner Yonghong Wang Stefan K Weber Paul M Winkler Yusheng Wu Mao Xiao Chao Yan Qing Ye Arttu Ylisirniö Marcel Zauner-Wieczorek Qiaozhi Zha Putian Zhou Richard C Flagan Joachim Curtius Urs Baltensperger Markku Kulmala Veli-Matti Kerminen Theo Kurtén Neil M Donahue Rainer Volkamer Jasper Kirkby Douglas R Worsnop Mikko Sipilä

Science 2021 02 4;371(6529):589-595. Epub 2021 Feb 4.

Institute for Atmospheric and Earth System Research/Physics, Faculty of Science, University of Helsinki, 00014 Helsinki, Finland.

Iodic acid (HIO) is known to form aerosol particles in coastal marine regions, but predicted nucleation and growth rates are lacking. Using the CERN CLOUD (Cosmics Leaving Outdoor Droplets) chamber, we find that the nucleation rates of HIO particles are rapid, even exceeding sulfuric acid-ammonia rates under similar conditions. We also find that ion-induced nucleation involves IO and the sequential addition of HIO and that it proceeds at the kinetic limit below +10°C. In contrast, neutral nucleation involves the repeated sequential addition of iodous acid (HIO) followed by HIO, showing that HIO plays a key stabilizing role. Freshly formed particles are composed almost entirely of HIO, which drives rapid particle growth at the kinetic limit. Our measurements indicate that iodine oxoacid particle formation can compete with sulfuric acid in pristine regions of the atmosphere.
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http://dx.doi.org/10.1126/science.abe0298DOI Listing
February 2021

ASFP (Artificial Intelligence based Scoring Function Platform): a web server for the development of customized scoring functions.

J Cheminform 2021 Feb 4;13(1). Epub 2021 Feb 4.

Hangzhou Institute of Innovative Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, China.

Virtual screening (VS) based on molecular docking has emerged as one of the mainstream technologies of drug discovery due to its low cost and high efficiency. However, the scoring functions (SFs) implemented in most docking programs are not always accurate enough and how to improve their prediction accuracy is still a big challenge. Here, we propose an integrated platform called ASFP, a web server for the development of customized SFs for structure-based VS. There are three main modules in ASFP: (1) the descriptor generation module that can generate up to 3437 descriptors for the modelling of protein-ligand interactions; (2) the AI-based SF construction module that can establish target-specific SFs based on the pre-generated descriptors through three machine learning (ML) techniques; (3) the online prediction module that provides some well-constructed target-specific SFs for VS and an additional generic SF for binding affinity prediction. Our methodology has been validated on several benchmark datasets. The target-specific SFs can achieve an average ROC AUC of 0.973 towards 32 targets and the generic SF can achieve the Pearson correlation coefficient of 0.81 on the PDBbind version 2016 core set. To sum up, the ASFP server is a powerful tool for structure-based VS.
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http://dx.doi.org/10.1186/s13321-021-00486-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7860246PMC
February 2021

Pilot deep RNA sequencing of worker blood samples from Singapore printing industry for occupational risk assessment.

NanoImpact 2020 Jul 13;19. Epub 2020 Aug 13.

Center for Nanotechnology and Nanotoxicology, Department of Environmental Health, T.H. Chan School of Public Health, Harvard University, Boston, MA 02115, USA.

Several engineered nanomaterials (ENMs) are used in toner-based printing equipment (TPE) including laser printers and photocopiers to improve toner performance. High concentration of airborne nanoparticles due to TPE emissions has been documented in copy centers and chamber studies. Recent animal inhalation studies by our group suggested exposure to laser printer-emitted nanoparticles (PEPs) increased cardiovascular risk by impairing ventricular performance and inducing hypertension and arrhythmia, consistent with global transcriptomic and metabolomic profiling results. There has been no genome-wide transcriptomic analysis of workers exposed to TPE emissions to systematically assess the occupational exposure health risks. In this pilot study, deep RNA sequencing of blood samples of workers in two printing companies in Singapore was performed. The genome-scale analysis of the blood samples from TPE exposed workers revealed perturbed transcriptional activities related to inflammatory and immune responses, metabolism, cardiovascular impairment, neurological diseases, oxidative stress, physical morphogenesis/deformation, and cancer, when compared with the control peers (office workers). Many of these disease risks associated with particle inhalation exposures in such work environments were consistent with the observation from the PEPs rat inhalation studies. In particular, the cell adhesion molecules (CAMs) was a top significantly perturbed pathway in blood samples from exposed workers compared with the office workers in both companies. The protein expression of sICAM was verified in plasma of exposed workers, showing a positive correlation with daily average nanoparticle concentration in indoor air measured in these two companies. Larger scale genomic and molecular epidemiology studies in copier operators are warranted in order to assess potential risks from such particulate matter exposures.
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http://dx.doi.org/10.1016/j.impact.2020.100248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840153PMC
July 2020

The mA methylome of SARS-CoV-2 in host cells.

Cell Res 2021 04 28;31(4):404-414. Epub 2021 Jan 28.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a global health emergency because of its rapid spread and high mortality. The molecular mechanism of interaction between host and viral genomic RNA is yet unclear. We demonstrate herein that SARS-CoV-2 genomic RNA, as well as the negative-sense RNA, is dynamically N-methyladenosine (mA)-modified in human and monkey cells. Combined RIP-seq and miCLIP analyses identified a total of 8 mA sites at single-base resolution in the genome. Especially, epidemic strains with mutations at these identified mA sites have emerged worldwide, and formed a unique cluster in the US as indicated by phylogenetic analysis. Further functional experiments showed that mA methylation negatively regulates SARS-CoV-2 infection. SARS-CoV-2 infection also triggered a global increase in host mA methylome, exhibiting altered localization and motifs of mA methylation in mRNAs. Altogether, our results identify mA as a dynamic epitranscriptomic mark mediating the virus-host interaction.
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http://dx.doi.org/10.1038/s41422-020-00465-7DOI Listing
April 2021

Early Segmental White Matter Fascicle Microstructural Damage Predicts the Corresponding Cognitive Domain Impairment in Cerebral Small Vessel Disease Patients by Automated Fiber Quantification.

Front Aging Neurosci 2020 11;12:598242. Epub 2021 Jan 11.

Department of Neurology, Drum Tower Hospital, Medical School and the State Key Laboratory of Pharmaceutical Biotechnology, Institute of Brain Science, Nanjing University, Nanjing, China.

To characterize earlier damage pattern of white matter (WM) microstructure in cerebral small vessel disease (CSVD) and its relationship with cognitive domain dysfunction. A total of 144 CSVD patients and 100 healthy controls who underwent neuropsychological measurements and diffusion tensor imaging (DTI) examination were recruited. Cognitive function, emotion, and gait were assessed in each participant. The automated fiber quantification (AFQ) technique was used to extract different fiber properties between groups, and partial correlation and general linear regression analyses were performed to assess the relationship between position-specific WM microstructure and cognitive function. Specific segments in the association fibers, commissural WM regions of interest (ROIs), and projection fibers were damaged in the CSVD group [ < 0.05, family-wise error (FWE) correction], and these damaged segments showed interhemispheric symmetry. In addition, the damage to specific tract profiles [including the posteromedial component of the right cingulum cingulate (CC), the occipital lobe portion of the callosum forceps major, the posterior portion of the left superior longitudinal fasciculus (SLF), and the bilateral anterior thalamic radiation (ATR)] was related to the dysfunction in specific cognitive domains. Among these tracts, we found the ATR to be the key set of tracts whose profiles were most associated with cognitive dysfunction. The left ATR was a specific fiber bundle associated with episode memory and language function, whereas the fractional anisotropy (FA) values of the intermediate component of the right ATR were negatively correlated with executive function and gait evaluation. It should be noted that the abovementioned relationships could not survive the Bonferroni correction ( < 0.05/27), so we chose more liberal uncorrected statistical thresholds. Damage to the WM fiber bundles showed extensive interhemispheric symmetry and was limited to particular segments in CSVD patients. Disruption of strategically located fibers was associated with different cognitive deficits, especially the bilateral ATR.
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http://dx.doi.org/10.3389/fnagi.2020.598242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829360PMC
January 2021

The predictive value of diabetic retinopathy on subsequent diabetic nephropathy in patients with type 2 diabetes: a systematic review and meta-analysis of prospective studies.

Ren Fail 2021 Dec;43(1):231-240

Division of Nephrology, Zhongshan Hospital, Xiamen University, Xiamen, PR China.

This systematic review and meta-analysis aimed to assess the predictive value of diabetic retinopathy (DR) on further diabetic nephropathy (DN) risk in patients with type 2 diabetes (T2D) based on the prospective cohort studies. PubMed, Embase, and the Cochrane Library were systematically searched for eligible prospective cohort studies through March 2020. The predictive value of DR was assessed using sensitivity, specificity, positive likelihood ratio (PLR) and negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the receiver operating characteristic curve (AUC) through the bivariate generalized linear mixed model and the random-effects model. Ten prospective cohort studies recruited 635 patients with T2D. The pooled sensitivity and specificity of DR for predicted DN were noted to be 0.64 (95% CI, 0.54-0.73) and 0.77 (95% CI, 0.60-0.88), respectively. The pooled PLR and NLR of DR for predicted DN were 2.72 (95% CI, 1.42-5.19) and 0.47 (95% CI, 0.33-0.67), respectively. The summary DOR for the relationship between DR and subsequent DN for T2D patients was 5.53 (95% CI, 2.00-15.30), and the AUC of DR for predicted DN was 0.73 (95% CI, 0.69-0.77). This study found significant associations between DR and subsequent DN risk for patients with T2D. Moreover, the predictive value of DR on subsequent DN risk was relatively lower.
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http://dx.doi.org/10.1080/0886022X.2020.1866010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7833016PMC
December 2021