Publications by authors named "Qing Ye"

918 Publications

Differential Expression and Prognostic Correlation of Immune Related Factors Between Right and Left Side Colorectal Cancer.

Front Oncol 2022 22;12:845765. Epub 2022 Jul 22.

Department of Pathology, The First Affiliated Hospital of University of Science and Technology of China (USTC), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Background: Growing evidence suggests that colorectal cancer (CRC) should be considered a heterogeneous disease. The right side (RCC) and left side (LCC) colorectal cancer have different clinical characteristics and immune landscapes. The aim of this study was to analyze differential expression and prognostic correlation of immune-related factors between RCC and LCC.

Methods: The gene expression profile and clinical characteristics of CRC patients were retrieved from The Cancer Genome Atlas data portal (n=525). Using a deconvolution algorithm, immune cell infiltration in RCC and LCC based on the RNA-seq data was analyzed. Differentially expressed genes (DEGs) were obtained by performing differential gene expression analysis. Immune-related DEGs were derived by the intersection with immune-related factors downloaded from the IMMPORT database. To further validate the findings, we applied immunohistochemical (IHC) staining of a CRC tissue microarray (TMA). The distribution of immune cells in RCC and LCC and changes in the expression of immune molecules on their membranes were verified. The expression levels of circulating cytokines were measured by flow cytometry to detect the cytokines secreted by immune cells in RCC and LCC. Furthermore, to reveal the prognostic value of differential immune factors on RCC and LCC patients, survival analysis based on mRNA levels using TCGA cohort and survival analysis using protein levels was performed using our CRC patients.

Results: The infiltration of immune cells differed between RCC and LCC, the infiltration degree of macrophages M0 was significantly higher in LCC, while the infiltration degree of differentiated macrophages M1 and M2, CD4+ T and CD8+ T cells was significantly higher in RCC. The expression of related molecules by immune cells also differed between RCC and LCC. The expression of 7 genes in RCC was higher than that in LCC, which were CCR5, CD209, CD8A, HCK, HLA-DPB1, HLA-DQA1, HLA-DRA, respectively. Meanwhile, the expression of 2 genes in LCC was higher than in RCC, which were IL-34 and PROCR. Patients with RCC having high expression of HLA-DQA1 mRNA or proteins had better survival and LCC patients with high expression of IL 34 mRNA or protein had better survival.

Conclusions: In this study, we comprehensively compared differences in immune cells and regulating factors between left and right colorectal cancer. Different expression patterns and their effects on survival were identified. The analysis of immune-related factors may provide a theoretical basis for precise immunotherapy of RCC and LCC.
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http://dx.doi.org/10.3389/fonc.2022.845765DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353740PMC
July 2022

Novel red light-emitting two-photon absorption compounds with large Stokes shifts for living cell imaging.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jul 25;282:121660. Epub 2022 Jul 25.

Department of Chemistry, Anhui Province Key Laboratory of Functional Inorganic Materials, Anhui University, Hefei 230039, PR China.

Three novel donor-π-acceptor two-photon absorption compounds (1PZPy, 2PZIm, 3CZPy) bearing the 10-butyl-10H-phenothiazine (9-butyl-9H-carbazole) donor, the pyridinium (benzimidazolium) acceptor, and the 2,5-divinylthiophene π-bridge were synthesized and fully characterized by H NMR, C NMR, FT-IR, and HRMS. The linear and nonlinear photophysical properties were systematically investigated. Their absorption properties show a strong solvent dependence, while the emission properties are nearly independent of solvent polarity. All of them possess large Stokes shifts (Δλ=149-190 nm in HO). 1PZPy and 3CZPy exhibit red fluorescence emission centered at about 635 and 660 nm, respectively. The two-photon absorption cross-sections measured by the open aperture Z-scan technique are determined to be 486 (1PZPy), 601 (2PZIm), and 753 GM (3CZPy) in DMF. The density functional theory calculations were further carried out to reveal their electronic structures. All the target compounds are verified to have low cytotoxicity in the working solution and good capability for one- and two-photon excitation fluorescence imaging, suggesting their potential application in bioimaging. Moreover, they show the organelle targeting ability in living cells with the high Pearson's coefficients above 0.94 for the endoplasmic reticulum.
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http://dx.doi.org/10.1016/j.saa.2022.121660DOI Listing
July 2022

Genotype-Phenotype Correlation Reanalysis in 83 Chinese Cases with Mutations.

Genet Res (Camb) 2022 19;2022:1473260. Epub 2022 Jul 19.

Department of Nephrology, The Children's Hospital of Zhejiang University School of Medicine, Hangzhou, China.

Background: Both Lowe syndrome and Dent-2 disease are caused by variants in the gene. However, the reason why patients with similar gene mutations presented with different phenotypes remains uncertain.

Methods: Children with hemizygous pathogenic or likely pathogenic variants in were compiled from published and unpublished consecutive cases from China. Furthermore, a Chi-square test was employed to analyze the correlation of the location and types of mutations on the phenotype of children with Lowe syndrome or Dent-2 disease.

Results: Among the total 83 patients, 70.8% (34/48) cases of Lowe syndrome presented with truncating mutations, while only 31.4% (11/35) cases of Dent-2 disease presented with truncating mutation (Χ = 12.662; < 0.001). Meanwhile, the majority of mutations in Dent-2 disease are located in Exon 2-12 (21/35, 60.0%), while the majority of mutations in Lowe syndrome are located in Exon 13-23 (39/48, 81.3%; Χ = 14.922; < 0.001).

Conclusions: Truncating mutations of the gene were more common in patients with Lowe syndrome than in Dent-2 disease, while mutation is more likely located at exon 2-12 in Dent-2 disease than that in Lowe syndrome. The type and location of mutation are important indicators for the phenotypes in patients with mutation. This is a large cohort study analyzing the genotype-phenotype correlation in patients with Lowe syndrome and Dent-2 disease in China. Our data may improve the interpretation of new variants and genetic counseling. Furthermore, a large international study would be necessary to illustrate the genotype-phenotype correlation in patients with mutations.
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http://dx.doi.org/10.1155/2022/1473260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325342PMC
August 2022

Life-cycle assessment of flue gas CO fixation from coal-fired power plant and coal chemical plant by microalgae.

Sci Total Environ 2022 Jul 30;848:157728. Epub 2022 Jul 30.

Microbial Sciences Institute, Department of Microbial Pathogenesis, Yale University, New Haven, CT 06511, USA. Electronic address:

The technology of flue gas CO fixation by microalgae is highly attractive in the era of CO neutrality. However, CO emission along the whole process has yet to be sufficiently evaluated. Here, a life-cycle assessment was performed to evaluate the energy conversion characteristics and environmental impacts of flue gas CO fixation from coal-fired power plant (Case 1) and coal chemical plant (Case 2) by microalgae. The results show that total energy consumption and CO gas emissions for Case 1 are 27.5-38.0 MJ/kg microalgae power (MP) and 5.7-7.7 kg CO equiv/kg MP, respectively, which are lower than that for Case 2 (122.5-181.3 MJ/kg MP and 32.7-48.6 kg CO equiv/kg MP). The CO gas aeration rate and microalgae growth rate are the two most sensitive parameters for the energy conversion and net CO emission. Therefore, increasing the CO aeration efficiency and microalgae growth rate are key to advance the technology of flue gas CO fixation by microalgae which will contribute to carbon naturality.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157728DOI Listing
July 2022

Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice.

J Clin Invest 2022 Aug;132(15)

Pittsburgh Institute of Brain Disorders and Recovery, and Department of Neurology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Immunomodulation holds therapeutic promise against brain injuries, but leveraging this approach requires a precise understanding of mechanisms. We report that CD8+CD122+CD49dlo T regulatory-like cells (CD8+ TRLs) are among the earliest lymphocytes to infiltrate mouse brains after ischemic stroke and temper inflammation; they also confer neuroprotection. TRL depletion worsened stroke outcomes, an effect reversed by CD8+ TRL reconstitution. The CXCR3/CXCL10 axis served as the brain-homing mechanism for CD8+ TRLs. Upon brain entry, CD8+ TRLs were reprogrammed to upregulate leukemia inhibitory factor (LIF) receptor, epidermal growth factor-like transforming growth factor (ETGF), and interleukin 10 (IL-10). LIF/LIF receptor interactions induced ETGF and IL-10 production in CD8+ TRLs. While IL-10 induction was important for the antiinflammatory effects of CD8+ TRLs, ETGF provided direct neuroprotection. Poststroke intravenous transfer of CD8+ TRLs reduced infarction, promoting long-term neurological recovery in young males or aged mice of both sexes. Thus, these unique CD8+ TRLs serve as early responders to rally defenses against stroke, offering fresh perspectives for clinical translation.
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http://dx.doi.org/10.1172/JCI157678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337834PMC
August 2022

Mucin 1 Inhibits Ferroptosis and Sensitizes Vitamin E to Alleviate Sepsis-Induced Acute Lung Injury through GSK3/Keap1-Nrf2-GPX4 Pathway.

Oxid Med Cell Longev 2022 21;2022:2405943. Epub 2022 Jul 21.

Department of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Ferroptosis is a nonapoptotic form of programmed cell death, which may be related to the occurrence and development of sepsis-induced acute respiratory distress syndrome (ARDS)/acute lung injury (ALI). Mucin 1 (MUC1) is a kind of macromolecule transmembrane glycoprotein. Previous studies have shown that MUC1 could relieve ALI in sepsis and predict whether sepsis patients would develop into ARDS. However, the role of MUC1 in the ferroptosis of sepsis-induced ALI/ARDS remains unclear.

Materials And Methods: Sera samples from 50 patients with sepsis/septic shock were used to detect iron metabolism-related markers. Western blot and qRT-PCR were conducted to detect the expression levels of ferroptosis-related genes. Enzyme-linked immunosorbent assay (ELISA) was performed to evaluate inflammatory factors. Transmission electron microscopy (TEM) was used to assess morphological changes of cells.

Results: The results showed that the iron metabolism-related indicators in sepsis-induced ARDS patients changed significantly, suggesting the iron metabolism disorder. The expression levels of ferroptosis-related genes in lung tissues of sepsis had marked changes, and the lipid peroxidation levels increased, while Ferrostatin-1 (Fer-1) could reverse the above results, which confirmed the occurrence of ferroptosis. In terms of mechanism studies, inhibition of MUC1 dimerization could increase the expression level of Keap1, reduce the phosphorylation level of GSK3, inhibit the entry of Nrf2 into the nucleus, further inhibit the expression level of GPX4, enhance the lipid peroxidation level of lung tissues, trigger ferroptosis, and aggravate lung injury. Besides, inhibiting MUC1 reversed the alleviating effect of vitamin E on ALI caused by sepsis, increased the aggregation of inflammatory cells in lung tissues, and aggravated alveolar injury and edema.

Conclusions: Our study was the first to explore the changes of iron metabolism indicators in ALI/ARDS of sepsis, clarify the important role of ferroptosis in ALI/ARDS induced by sepsis, and reveal the effects and specific mechanisms of MUC1 in regulating ferroptosis, as well as the sensitization on vitamin E.
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http://dx.doi.org/10.1155/2022/2405943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334047PMC
August 2022

Association of Environmental Elements with Respondents' Behaviors in Open Spaces Using the Direct Gradient Analysis Method: A Case Study of Jining, China.

Int J Environ Res Public Health 2022 Jul 12;19(14). Epub 2022 Jul 12.

School of Civil Engineering and Architecture, University of Jinan, Jinan 250022, China.

Following rapid urban development, higher demands are now being placed on urban open spaces in China, and the relationship between environmental elements and respondents' behaviors in open spaces has become a common concern for researchers. Current research using geographic information systems has yielded macroscopic portraits of the behavioral trends and outcomes of research subjects, but evaluating their actual needs is complex. This paper proposes a new method to analyze the relationship between open spaces and respondents' behaviors from a detailed perspective. Direct gradient analysis was employed with stratified sampling to select sample points in open spaces. Environment quality, ancillary facilities, and canal culture were selected as subjective evaluation factors. The greatest advantage of the proposed procedure is that it produces a ranking diagram, which compensates for the shortcomings of research methods that cannot directly express the actual needs related to respondents' behaviors. From a case study in Jining, China, a location's environmental quality and ancillary facilities were found to have the greatest influence on the behaviors of those using open spaces. Finally, strategies for improving environmental quality in open spaces are proposed.
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http://dx.doi.org/10.3390/ijerph19148494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316188PMC
July 2022

Rational development of a combined mRNA vaccine against COVID-19 and influenza.

NPJ Vaccines 2022 Jul 26;7(1):84. Epub 2022 Jul 26.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.

As the world continues to experience the COVID-19 pandemic, seasonal influenza remain a cause of severe morbidity and mortality globally. Worse yet, coinfection with SARS-CoV-2 and influenza A virus (IAV) leads to more severe clinical outcomes. The development of a combined vaccine against both COVID-19 and influenza is thus of high priority. Based on our established lipid nanoparticle (LNP)-encapsulated mRNA vaccine platform, we developed and characterized a novel mRNA vaccine encoding the HA antigen of influenza A (H1N1) virus, termed ARIAV. Then, ARIAV was combined with our COVID-19 mRNA vaccine ARCoV, which encodes the receptor-binding domain (RBD) of the SARS-CoV-2 S protein, to formulate the final combined vaccine, AR-CoV/IAV. Further characterization demonstrated that immunization with two doses of AR-CoV/IAV elicited robust protective antibodies as well as antigen-specific cellular immune responses against SARS-CoV-2 and IAV. More importantly, AR-CoV/IAV immunization protected mice from coinfection with IAV and the SARS-CoV-2 Alpha and Delta variants. Our results highlight the potential of the LNP-mRNA vaccine platform in preventing COVID-19 and influenza, as well as other respiratory diseases.
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http://dx.doi.org/10.1038/s41541-022-00478-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315335PMC
July 2022

RBCK1 promotes hepatocellular carcinoma metastasis and growth by stabilizing RNF31.

Cell Death Discov 2022 Jul 22;8(1):334. Epub 2022 Jul 22.

The Third Clinical Medical College, Fujian Medical University, Fuzhou, China.

RNF31 (HOIP), RBCK1 (HOIL-1L), and SHARPIN are subunits of the linear ubiquitin chain assembly complex. Their function and specific molecular mechanisms in hepatocellular carcinoma (HCC) have not been reported previously. Here, we investigated the role of RNF31 and RBCK1 in HCC. We showed that RNF31 and RBCK1 were overexpressed in HCC and that upregulation of RNF31 and RBCK1 indicated poor clinical outcomes in patients with HCC. RNF31 overexpression was significantly associated with more satellite foci and vascular invasion in patients with HCC. Additionally, RBCK1 expression correlated positively with RNF31 expression in HCC tissues. Functionally, RBCK1 and RNF31 promote the metastasis and growth of HCC cells. Moreover, the RNF31 inhibitor gliotoxin inhibited the malignant behavior of HCC cells. Mechanistically, RBCK1 interacted with RNF31 and repressed its ubiquitination and proteasomal degradation. In summary, the present study revealed an oncogenic role and regulatory relationship between RBCK1 and RNF31 in facilitating proliferation and metastasis in HCC, suggesting that they are potential prognostic markers and therapeutic targets for HCC.
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http://dx.doi.org/10.1038/s41420-022-01126-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307510PMC
July 2022

The Evaluation of a SEER-Based Nomogram in Predicting the Survival of Patients Treated with Neoadjuvant Therapy Followed by Esophagectomy.

Front Surg 2022 29;9:853093. Epub 2022 Jun 29.

Department of Thoracic Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai China.

Background: A novel nomogram based on the Surveillance, Epidemiology, and End Results (SEER) database has been developed to predict the survival of patients with esophageal carcinoma who received neoadjuvant therapy followed by surgery. We aimed to evaluate the accuracy and value of the nomogram with an external validation cohort.

Methods: A total of 2,224 patients in SEER database were divided into the training cohort ( = 1556) and the internal validation cohort ( = 668), while 77 patients in our institute were enrolled in the external validation cohort. A Cox proportional hazards regression model was used to develop a nomogram based on the training cohort, while the C-indexes, the calibration curves, receiver operating characteristics curve (ROC), and Kaplan-Meier survival curve were applied in the internal and external validation cohort.

Results: Five independent risk factors were identified and integrated into the nomogram (C-index = 0.645, 95%CI 0.627-0.663). The nomogram exhibited good prognostic value in the internal validation cohort (C-index = 0.648 95%CI 0.622-0.674). However, the C-index, calibration plot, receiver operating characteristics curve (ROC) analysis, Kaplan-Meier survival curve of the nomogram in the external validation cohort were not as good as the training and internal validation cohort (C-index = 0.584 95%CI 0.445-0.723). Further analysis demonstrated that the resection margin involvement (R0, R1, or R2 resection) was an independent risk factor for the patients, which was not included in the SEER cohort.

Conclusions: the nomogram based on the SEER database fails to accurately predict the prognosis of the patients in the external validation cohort, which can be caused by the absence of essential information from the SEER database.
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http://dx.doi.org/10.3389/fsurg.2022.853093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276989PMC
June 2022

MIP-1 Level and Its Correlation with the Risk of Left Atrial Remodeling in Patients with Atrial Fibrillation.

Contrast Media Mol Imaging 2022 24;2022:1756268. Epub 2022 Jun 24.

Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.

The aim of this study is to investigate the expression level of macrophage inflammatory protein-1 (MIP-1) in atrial fibrillation patients and its correlation with the risk of left atrial remodeling. A total of 64 atrial fibrillation patients admitted to our hospital from April 2020 to December 2021 were prospectively selected as the case group and 61 healthy subjects who received physical examination during the same period were selected as the control group. Serum MIP-1 level was determined by a double-antibody sandwich enzyme-linked immunosorbent assay. Serum MIP-1 expression levels were compared between the case and the control groups. The case group was divided into high-level and low-level groups according to the serum MIP-1 median. Simultaneously, the sociodemographic data, clinical data, and left atrial remodeling indexes of the patients were collected in the case group. The Pearson correlation analysis was applied to analyze the correlation between the serum MIP-1 level and the risk of left atrial remodeling in patients with atrial fibrillation. The serum MIP-1 level was significantly higher in the case group than that in the control group ( < 0.05), high-level group (≥2.14 pg/mL, 32 cases), and low-level group (<2.14 pg/mL, 32 cases). There were significant differences in the anteroposterior diameter, upper and lower diameter, left and right diameter of the left atrium, left atrial volume, volume index, left atrial global ejection fraction, and sphericity between the low-level and high-level groups ( < 0.05). The Pearson correlation analysis showed that serum MIP-1 level was positively correlated with the left atrial anteroposterior diameter ( = 0.745), left atrial left and right diameter ( = 0.759), left atrial upper and lower diameter ( = 0.810), left atrial volume ( = 0.837), left atrial volume index ( = 0.813), and left atrial sphericity ( = 0.785) but negatively correlated with the left atrial global ejection fraction ( = -0.731) ( < 0.05). The expression level of serum MIP-1 is high in atrial fibrillation patients and is associated with the risk of left atrial remodeling.
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http://dx.doi.org/10.1155/2022/1756268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249486PMC
July 2022

Evaluation of nitroreductase activity in nasopharyngeal carcinoma progression by an activatable two-photon fluorescent probe.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jul 11;281:121616. Epub 2022 Jul 11.

Shengli Clinical Medical College of Fujian Medical University, Department of Otolaryngology, Head and Neck Surgery, Fujian Provincial Hospital, Fuzhou 350001, China. Electronic address:

Nasopharyngeal carcinoma (NPC) originating from the epithelium cells is the most common malignant tumor of the head and neck. Small-molecule fluorescent probes for early diagnosis of NPC can effectively improve the 5-year survival rate of patients, which makes it become a research hotspot in recent years. Previous studies have suggested the expression levels of NTR in hypoxic tissues or cells and tumors increased relative to the normal state and were positively correlated with the degree of hypoxia. Regarding the mentioned above, we designed a two-photon fluorescent probe NaT-NTR for the detection of NTR in nasopharyngeal cell lines and tissues at different hypoxia levels. NaT-NTR showed high selectivity and sensitivity toward NTR in a complex physiological environment. Furthermore, imaging NTR in different cell lines revealed that the level of intracellular NTR might be positively correlated with the malignancy of nasopharyngeal carcinoma. More importantly, NaT-NTR was successfully applied to detect and image NTR in human nasopharyngeal carcinoma with a penetration depth of 100 µm. On this basis, NaT-NTR might be a powerful chemical tool for the early diagnosis of nasopharyngeal carcinoma.
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http://dx.doi.org/10.1016/j.saa.2022.121616DOI Listing
July 2022

The Enhanced Interhemispheric Functional Connectivity in the Striatum Is Related to the Cognitive Impairment in Individuals With White Matter Hyperintensities.

Front Neurosci 2022 28;16:899473. Epub 2022 Jun 28.

State Key Laboratory of Pharmaceutical Biotechnology, Department of Neurology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.

Objective: The cognitive performance of individuals with white matter hyperintensities (WMH) tends to vary considerably. This study aimed to explore the relationship of the synchronous spontaneous activities in homotopic areas across hemispheres, named as voxel-mirrored homotopic connectivity (VMHC), with the cognitive performance of individuals with WMH.

Materials And Methods: Eighty-two WMH subjects without cognitive impairment (CI), 56 WMH subjects with CI, and 92 healthy subjects (HS) underwent neuropsychological tests and multimodal magnetic resonance imaging scans. VMHC maps were analyzed among the three groups. Correlative analyses were performed between VMHC values and cognitive function.

Results: No significant difference in WMH volume, brain volume, or gray matter atrophy rate was shown between WMH subjects with and without CI. In contrast, those with CI displayed lower VMHC in the bilateral cuneus and calcarine and higher VMHC in the lentiform nucleus and caudate nucleus (LNCN) than those without CI. Furthermore, the VMHC in the LNCN was negatively associated with the global function and the memory function in WMH subjects.

Conclusion: The enhanced VMHC in the LNCN was associated with the development of CI in individuals with WMH. This finding may contribute to the exploration of surrogate markers for the CI caused by WMH.
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http://dx.doi.org/10.3389/fnins.2022.899473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273905PMC
June 2022

The Diagnostic Potential of and DNA Methylation in Early Lung Adenocarcinoma.

Front Oncol 2022 28;12:849024. Epub 2022 Jun 28.

Department of Pathology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Objective: Methylation of the promoters of and are potentially informative biomarkers for the diagnosis of early lung adenocarcinoma (LUAD). Abnormal methylation of and promoters may promote the occurrence and facilitate the progression of LUAD.

Materials And Methods: We selected 54 patients with early LUAD and 31 patients with benign lung nodules as a NJDT cohort and evaluated their DNA methylation and mRNA sequencing levels. The DNA methylation sequencing, mRNA sequencing, and clinical data for patients with LUAD were obtained from The Cancer Genome Atlas, and served as a TCGA cohort. We evaluated the diagnostic potential of a and combined promoter methylation assay for detection of early LUAD in the NJDT cohort. Then we explored the promoter methylation levels of and and their gene expression between normal and tumor samples at different stages in both cohorts. Pathways enriched between tumor and normal samples of methylation-positive patients in the NJDT cohort were analyzed.

Results: In the NJDT cohort, the sensitivity of the combined promoter methylation assay on tumor samples was 74.07%, the sensitivity on paired tumor and paracancerous samples was 77.78%, and the specificities in both contexts were 100%. The combined promoter methylation-positive patients had clinicopathologic features including older age, larger tumors, deeper invasion, and higher Ki-67 expression. In both cohorts, expression increased and expression decreased in tumor samples. The promoter methylation level of and was significantly higher in tumor samples at stage I-II than that in normal samples. The promoter methylation levels of these two genes were both negative associated with their expression in early tumor samples. In the NJDT cohort, methylation-positive patients of both individual and assays exhibited upregulation of folate acid metabolism and nucleotide metabolism in tumor samples. The methylation-positive and methylation-positive patients showed the downregulation of pathways related to cell proliferation and apoptosis and pathways involved in DNA repair, cell growth and cell adhesion, respectively.

Conclusion: The combined promoter methylation assay for and can be used for screening and diagnosis of early LUAD, with good sensitivity and specificity. The promoter methylation levels of and were associated with their abnormal mRNA expression, and affected DNA instability, cell proliferation, apoptosis and tumor microenvironment in patients with LUAD.
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http://dx.doi.org/10.3389/fonc.2022.849024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273978PMC
June 2022

SARS-CoV-2 Spreads Globally Through the Object-to-Human Transmission of Cross-Border Logistics.

Authors:
Wenxia Shao Qing Ye

Front Microbiol 2022 23;13:918957. Epub 2022 Jun 23.

Department of Clinical Laboratory, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China.

With globalization, the demand for transnational logistics is growing rapidly. However, the object-to-human transmission of SARS-CoV-2 has been reported in transnational logistics production, transportation, storage, sales, and consumption. Every link of transnational logistics has the risk of spreading the COVID-19 pandemic. It is concluded that low temperatures, dry environments, and smooth surfaces are conducive to the long-term survival of SARS-CoV-2 on the surface of transnational goods. Epidemiological investigation and big data analysis show that the object-to-human transmission route of direct contact with contaminated cold chain goods plays a key role in the outbreak and transmission of the COVID-19 pandemic. This may be the most crucial reason for the global spread of SARS-CoV-2 caused by transnational logistics. It is an effective way to prevent the spread of SARS-CoV-2 from object-to-human through transnational logistics by strengthening the management of employees in all aspects of transnational logistics, carrying out comprehensive disinfection and quarantine of and guiding consumers to handle transnational goods properly.
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http://dx.doi.org/10.3389/fmicb.2022.918957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260597PMC
June 2022

Development of Enzyme-Free DNA Amplifier Based on Chain Reaction Principle.

Acta Biomater 2022 Jul 8. Epub 2022 Jul 8.

School of Medicine, Nankai University, Tianjin 300071, China; Nankai University Eye Institute, Nankai University, Tianjin 300071, China. Electronic address:

Enzyme-free DNA amplifiers can amplify the signal of nucleic acid molecules. They can be applied to DNA molecular operation and nucleic acid detection. The reaction speed is the core index to evaluate DNA amplifiers. In this study, we designed a DNA amplifier based on an enzyme-free chain reaction. This DNA amplifier can release one more signal molecule in each round of reaction and trigger the next round, which significantly improved reaction speed. Moreover, because the amplifier used a stable DNA structure, the reaction can occur at room temperature. To integrate the amplifier into other DNA molecular operations, we performed the amplification reaction in a microfluidic chip module. The results showed that the amplifier can realize real-time signal feedback at a proper input molecule concentration and reach the endpoint in 40 s, even at a low relative concentration. To apply the amplifier for nucleic acid detection, we also used a conventional fluorescent polymerase chain reaction instrument for the reaction. The results showed that the amplifier specifically detected trace DNA single-stranded molecules. To solve the leakage problem of existing amplifiers, we designed a DNA molecule as the chain reaction's inhibitor, which was crucial in controlling the reaction speed and preventing leakage. STATEMENT OF SIGNIFICANCE: Traditional amplifier strategies of enzyme-free DNA amplifiers relied on a constant number of cycling molecules to catalyze the amplifier molecules' changing structure and release fluorescent signals, which lead low reaction speed. Based on an enzyme-free chain reaction, we designed a DNA amplifier which can release one more cycling molecule in each loop and trigger the next loop and significantly improve reaction speed in this study. Our analysis on microfluidic chip module and PCR instrument verifies high sensitivity and selectivity. And this strategy of DNA amplifier realizes the control of reaction and prevents leakage. We believe that this automated amplification strategy could have great applications in vivo signal detection, imaging, and signal molecule translation.
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http://dx.doi.org/10.1016/j.actbio.2022.06.047DOI Listing
July 2022

Single B Cell Gene Co-Expression Networks Implicated in Prognosis, Proliferation, and Therapeutic Responses in Non-Small Cell Lung Cancer Bulk Tumors.

Cancers (Basel) 2022 Jun 25;14(13). Epub 2022 Jun 25.

West Virginia University Cancer Institute, Morgantown, WV 26506, USA.

In NSCLC, there is a pressing need for immunotherapy predictive biomarkers. The processes underlying B-cell dysfunction, as well as their prognostic importance in NSCLC, are unknown. Tumor-specific B-cell gene co-expression networks were constructed by comparing the Boolean implication modeling of single-cell RNA sequencing of NSCLC tumor B cells and normal B cells. Proliferation genes were selected from the networks using in vitro CRISPR-Cas9/RNA interfering (RNAi) screening data in more than 92 human NSCLC epithelial cell lines. The prognostic and predictive evaluation was performed using public NSCLC transcriptome and proteome profiles. A B cell proliferation and prognostic gene co-expression network was present only in normal lung B cells and missing in NSCLC tumor B cells. A nine-gene signature was identified from this B cell network that provided accurate prognostic stratification using bulk NSCLC tumor transcriptome ( = 1313) and proteome profiles ( = 103). Multiple genes (, , , and ) differentially expressed in NSCLC B cells, peripheral blood lymphocytes, and tumor T cells had concordant prognostic indications at the mRNA and protein expression levels. The selected genes were associated with drug sensitivity/resistance to 10 commonly used NSCLC therapeutic regimens. Lestaurtinib was discovered as a potential repositioning drug for treating NSCLC.
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http://dx.doi.org/10.3390/cancers14133123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265014PMC
June 2022

Radiofrequency Ablation in Patients Undergoing Mitral Valve Surgery with or without Giant Left Atria.

Ann Thorac Cardiovasc Surg 2022 Jul 6. Epub 2022 Jul 6.

Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Purpose: We aimed to compare the efficacy of radiofrequency ablation (RFA) in patients undergoing mitral valve (MV) surgery with or without giant left atria.

Methods: This retrospective, single-center, cohort study investigated patients who underwent MV surgery and concomitant RFA from 2009 to 2019. Patients were divided into non-giant left atria (diameter ≤65 mm, n = 1543) and giant left atria (diameter >65 mm, n = 241) groups. Five-year freedom from atrial tachyarrhythmia recurrence and thromboembolic event (TE) rates were assessed with death as the competing risk factor with and without propensity-score matching.

Results: Patients with giant left atria had higher mortality (10.8% versus 6.2%, P = 0.008) and readmission rates for heart failure than those without (12.0% versus 6.8%, P = 0.004). Atrial tachyarrhythmia recurrence rates were higher in patients with giant left atria than in those without (49% versus 24% at 5 years, P <0.001), but the cumulative incidence of TEs before (P = 0.944) and after (P = 0.695) propensity-score matching was comparable.

Conclusions: RFA effectively prevented TEs in patients with giant left atria, despite significant atrial tachyarrhythmia recurrence. Atrial tachyarrhythmia recurrence did not increase the risk of TEs. A lower success rate should be considered when deciding whether to perform surgical ablation in patients with giant left atria.
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http://dx.doi.org/10.5761/atcs.oa.22-00033DOI Listing
July 2022

Ångstrom-scale silver particles potently combat SARS-CoV-2 infection by suppressing the ACE2 expression and inflammatory responses.

J Mater Chem B 2022 Jul 20;10(28):5454-5464. Epub 2022 Jul 20.

Institute of Virology and AIDS Research, Key laboratory of Organ Regeneration and Transplantation of The Ministry of Education, The First Hospital of Jilin University, Changchun, Jilin 130021, China.

The SARS-CoV-2 pandemic has become a severe global public health event, and the development of protective and therapeutic strategies is urgently needed. Downregulation of angiotensin converting enzyme 2 (ACE2; one of the important SARS-CoV-2 entry receptors) and aberrant inflammatory responses (cytokine storm) are the main targets to inhibit and control COVID-19 invasion. Silver nanomaterials have well-known pharmaceutical properties, including antiviral, antibacterial, and anticancer properties. Here, based on a self-established metal evaporation-condensation-size graded collection system, smaller silver particles reaching the Ångstrom scale (AgÅPs) were fabricated and coated with fructose to obtain a stabilized AgÅP solution (F-AgÅPs). F-AgÅPs potently inactivated SARS-CoV-2 and prevented viral infection. Considering the application of anti-SARS-CoV-2, a sterilized F-AgÅP solution was produced spray formulation. In our model, the F-AgÅP spray downregulated ACE2 expression and attenuated proinflammatory factors. Moreover, F-AgÅPs were found to be rapidly eliminated to avoid respiratory and systemic toxicity in this study as well as our previous studies. This work presents a safe and potent anti-SARS-CoV-2 agent using an F-AgÅP spray.
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http://dx.doi.org/10.1039/d2tb00336hDOI Listing
July 2022

An Intelligent Fault Diagnosis Based on Adversarial Generating Module and Semi-supervised Convolutional Neural Network.

Comput Intell Neurosci 2022 24;2022:1679836. Epub 2022 Jun 24.

General Office, Yangtze University, Jingzhou 430023, China.

Aiming at the existing problems in machinery monitoring data such as high cost of labeling and lack of typical failure samples, this paper launches a research on the semi-supervised-style intelligent fault diagnosis. Taking a great mount of unlabeled data and only a small quantity of labeled data as inputs, a novel fault diagnosis framework based on adversarial generating module and semi-supervised convolutional neural network (SSCNN) is proposed. Firstly, a semi-supervised learning module based on manifold-regularization-based fuzzy clustering discrimination (MRFCD) is proposed to make full use of the valuable fault-related information contained in unlabeled data. Secondly, MRFCD was introduced into CNN to construct pseudo-labels and estimate the objective function of unlabeled data. Then, the semi-supervised deep-learning-module-based MRFCD-SSCNN is established. Thirdly, to enhance the effect of MRFCD-SSCNN, generative adversarial network (GAN) was utilized to increase the size of training data under failure conditions. The framework based on GAN-MRFCD-SSCNN is proposed to achieve semi-supervised style intelligent fault diagnosis. To verify the performance of the diagnostic framework, vibrational signals of main reducer collected from actual test rig are employed. The comparative results confirm that the proposed framework outperforms some classical semi-supervised diagnostic models and achieves the accuracy of 96.2% using only 400 labeled samples.
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http://dx.doi.org/10.1155/2022/1679836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249457PMC
July 2022

A sliver deposition signal-enhanced optical biomolecular detection device based on reduced graphene oxide.

Talanta 2022 Jun 17;249:123691. Epub 2022 Jun 17.

Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics and TEDA Applied Physics, Nankai University, Tianjin, 300071, PR China. Electronic address:

The development of high-sensitive biomolecular detection system is of great significance for diseases early diagnosis. The novel optical sensor based on the polarization-sensitive absorption of graphene has a great potential in biological detection. However, the detection sensitivity of the device can hardly meet the needs of clinical analysis currently. This study applies sliver deposition signal amplification to the optical biomolecular detection device based on reduced graphene oxide for the sensitive immunoassay. In redox cycling enzymatic silver deposition reaction, the more alkaline phosphatase label bound on chip surface will cause a faster silver deposition rate. The specific antibody detection confirms that the sliver deposition can enhance the detection signal significantly. In cardiac biomarker Creatine Kinase-MB measurement, the minimum detection concentration is 0.1 ng/mL. To be more important, within the range from detection limit to 10 ng/mL, the signal intensity is highly correlated with target protein concentration, so the biomolecular detection device can meet clinical assay requirements. The signal-enhanced optical biomolecular detection device based on reduced graphene oxide shows excellent sensitivity and selectivity, and provides a new strategy for biomolecules detection, which can be applied in diseases accurate prediction and diagnosis.
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http://dx.doi.org/10.1016/j.talanta.2022.123691DOI Listing
June 2022

A critical role of the podocyte cytoskeleton in the pathogenesis of glomerular proteinuria and autoimmune podocytopathies.

Acta Physiol (Oxf) 2022 Aug 28;235(4):e13850. Epub 2022 Jun 28.

Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China.

Selective glomerular filtration relies on the membrane separating the glomerular arterioles from the Bowman space. As a major component of the glomerular filtration barrier, podocytes form foot processes by the actin cytoskeleton, which dynamically adjusts in response to environmental changes to maintain filtration barrier integrity. The slit diaphragms bridge the filtration slits between neighboring foot processes and act as signaling hubs interacting with the actin cytoskeleton. Focal adhesions relay signals to regulate actin dynamics while allowing podocyte adherence to the basement membrane. Mutations in actin regulatory and signaling proteins may disrupt the actin cytoskeleton, resulting in foot process retraction, effacement, and proteinuria. Large-scale gene expression profiling platforms, transgenic animal models, and other in vivo gene delivery methods now enhance our understanding of the interactions among podocyte focal adhesions, slit diaphragms, and actin dynamics. In addition, our team found that at least 66% of idiopathic nephrotic syndrome (INS) children have podocyte autoantibodies, which was defined as a new disease subgroup-, autoimmune podocytopathies. This review outlines the pathophysiological mechanisms of podocyte cytoskeleton protein interactions in proteinuria and glomerular podocytopathy.
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http://dx.doi.org/10.1111/apha.13850DOI Listing
August 2022

A Three-Dimensional Inorganic Analogue of 9,10-Diazido-9,10-Diboraanthracene: A Lewis Superacidic Azido Borane with Reactivity and Stability.

Angew Chem Int Ed Engl 2022 Jun 17:e202205506. Epub 2022 Jun 17.

Department of Chemistry, Southern University of Science and Technology, 518055, Shenzhen, P. R. China.

Herein, we report the facile synthesis of a three-dimensional (3D) inorganic analogue of 9,10-diazido-9,10-dihydrodiboraantracene, which turned out to be a monomer in both the solid and solution state, and thermally stable up to 230 °C, representing a rare example of azido borane with boosted Lewis acidity and stability in one. Apart from the classical acid-base and Staudinger reactions, E-H bond activation (E=B, Si, Ge) was investigated. While the reaction with B-H (9-borabicyclo[3.3.1]nonane) led directly to the 1,1-addition on N upon N elimination, the Si-H (Et SiH, PhMe SiH) activation proceeded stepwise via 1,2-addition, with the key intermediates 5 and 6 being isolated and characterized. In contrast, the cooperative Ge-H was reversible and stayed at the 1,2-addition step.
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http://dx.doi.org/10.1002/anie.202205506DOI Listing
June 2022

Traditional Chinese medicine syndrome differentiation and treatment by stages of Parkinson's disease: study protocol for a multicentre, randomized, double-blind, placebo-controlled clinical trial.

Chin Med 2022 Jun 13;17(1):68. Epub 2022 Jun 13.

Encephalopathy Department, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, No. 725, South Wanping Road, Shanghai, China.

Background: Parkinson's disease (PD) is a progressive neurodegenerative disease common in aged populations. Classified by Hoehn & Yahr stages, patients are often divided into mild/early stage, moderate/middle stage, and advanced/late stage. With disease progression, PD shows high heterogeneity in each stage. Based on traditional Chinese medicine (TCM) syndrome differentiation theory and our previous works, we found that during the early stage, the main syndrome is Yin deficiency of the liver and kidney; during the moderate stage, the main syndromes are phlegm heat and wind stirring and blood stasis and wind stirring; and during the late stage, the dominant syndromes are deficiency of Yin and Yang and deficiency of Qi and blood. Hence, we proposed a new model of TCM treatment by the stage of PD. Based on Shudi Pingchan formula, an experimental formula of our team, we developed Ziyin Pingchan formula, Jiedu Pingchan formula, and Fuzheng Pingchan formula to treat each stage. This study is designed to evaluate the therapeutic effect of treating Parkinson's disease by stages using traditional Chinese medicine and to provide an evidence base for forming a standardized scheme of diagnosis and treatment.

Methods: This study is designed as a multicentre, randomized, double-blind, placebo-controlled clinical trial. Patients will be stratified into 3 subgroups according to Hoehn & Yahr stage; 172, 168, and 72 participants will be required to be in the mild PD, moderate PD, and advanced PD subgroups, respectively, and will be randomized into the treatment or control group at a 1:1 ratio. The mild PD subgroup will receive a 48-week intervention, and the other 2 groups will receive a 24-week intervention. All groups will have a follow-up visit 12 weeks after starting the intervention. The intervention group will receive the Ziyin Pingchan formula, Jiedu Pingchan formula, or Fuzheng Pingchan formula, and the control group will receive the corresponding placebo. The primary outcomes will be the first addition of levodopa for the mild PD subgroup, the duration of the "OFF" period for the moderate PD subgroup, and the Parkinson's Disease Questionnaire (PDQ-39) for the advanced PD subgroup. The secondary outcomes will also be verified by subgroups, including the Unified Parkinson's Disease Rating Scale (UPDRS), Parkinson's Disease Sleep Scale-2 (PDSS-2), scales for Outcomes in Parkinson's Disease-Autonomic (SCOPA-AUT), and the nonmotor symptom scale (NMSS).

Expected Outcomes: To our knowledge, this is the first trial to combine TCM syndrome differentiation with PD clinical stages and put it into clinical practice. The results of this trial will provide clinical evidence for the therapeutic effect of TCM formulas on PD patients of all stages and help build a new TCM treatment by stage model of PD.

Trial Registration: This trial is registered at the Chinese Clinical Trial Registry ( http://www.chictr.org.cn/ ).

Registration Number: ChiCTR2200056373, Date: 2022-02-04, version 1.
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http://dx.doi.org/10.1186/s13020-022-00625-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9190110PMC
June 2022

Comprehensive genomic analysis of primary malignant melanoma of the esophagus reveals similar genetic patterns compared with epithelium-associated melanomas.

Mod Pathol 2022 Jun 10. Epub 2022 Jun 10.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery II, Peking University Cancer Hospital & Institute, Beijing, 100142, China.

Primary malignant melanoma of the esophagus (PMME) is an exceedingly rare disease with a poor prognosis. The etiology of PMME remains largely unknown and genetic characteristics are yet to be clarified, essential for identifying potential therapeutic targets and defining treatment guidelines. Here, we performed whole-exome sequencing on 47 formalin-fixed paraffin-embedded specimens from 18 patients with PMME, including 23 tumor samples, 6 metastatic lymph nodes, and 18 tumor-adjacent normal tissues. The genomic features of PMME were comprehensively characterized, and comparative genomic analysis was further performed between these specimens and 398 skin cutaneous melanomas (SKCM), 67 non-esophagus mucosal melanomas (NEMM), and 79 uveal melanomas (UVM). In the PMME cohort, recurrently mutated driver genes, such as MUC16, RANBP2, NRAS, TP53, PTPRT, NF1, MUC4, KMT2C, and BRAF, were identified. All RANBP2 mutations were putatively deleterious, and most affected samples had multipoint mutations. Furthermore, RANBP2 showed parallel evolution by multiregional analysis. Whole-genome doubling was an early truncal event that occurred before most driver mutations, except for in TP53. An ultraviolet radiation-related mutational signature, SBS38, was identified as specific to epithelial melanomas and could predict inferior survival outcomes in both PMME and SKCM patients. Comparing the mutational and copy number landscapes between PMME and other subtypes of melanoma revealed that PMME has a similar genomic pattern and biological characteristics to SKCM. In summary, we comprehensively defined the key genomic aberrations and mutational processes driving PMME and suggested for the first time that PMME may share similar genomic patterns with SKCM; therefore, patients with rare melanomas, such as PMME, may benefit from the current treatment used for common cutaneous melanoma.
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http://dx.doi.org/10.1038/s41379-022-01116-5DOI Listing
June 2022

Longitudinal genomic alternations and clonal dynamics analysis of primary malignant melanoma of the esophagus.

Neoplasia 2022 08 1;30:100811. Epub 2022 Jun 1.

The Precision Medicine Centre of Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, Jiangsu, China; Department of Pathology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230036, China; Intelligent Pathology Institute, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230036, China. Electronic address:

Primary malignant melanoma of the esophagus (PMME) is a rare gastrointestinal melanoma with a high rate of recurrence and metastasis. The standard of care for PMME has not been established yet due to a lack of understanding of its clinical and molecular pathogenesis. Thus, we performed genomic profiling on a recurrent PMME case to seek novel opportunities for the management of this rare disease. Between 2013 and 2016, 6 tissue samples including 3 from the primary tumors, 2 from the relapsed tumors, and 1 from a normal control were collected from a patient diagnosed with PMME and were subjected to whole-exome sequencing to track the dynamic genetic changes. Additionally, we also analyzed a cohort of 398 samples obtained from the TCGA skin cutanesous melanoma (TCGA-SKCM) dataset to assess the frequency and determine the clinical implications of genomic events found in the presented study. ARHGAP35 (p.L1022M) was the only mutation shared across temporal PMME lesions. The PMME samples showed higher levels of genetic instability and intra-tumor heterogeneity. They also shared several concordant copy number variations (CNV). All lesions were concordant with the evolution trajectory, and shrinkage of the founding clone caused the subclonal population to become dominant in PT1c, which was likely the reason behind metastatic seeding. ARHGAP35 mutations were found in 6% of the TCGA-SKCM cohort samples. The presence of the mutations was associated with poor progression-free survival (PFS) by both univariate and multivariate Cox regression analyses. Our study showed that the primary tumor clone disseminates earlier in PMME. This highlights the need to understand the mechanism involved in the early PMME recurrence to optimize treatment.
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http://dx.doi.org/10.1016/j.neo.2022.100811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166384PMC
August 2022

Synthesis of Iminoboryl -Carboranes by Lewis Base Promoted Aminoborirane-to-Iminoborane Isomerization.

Inorg Chem 2022 Jun 1;61(23):8879-8886. Epub 2022 Jun 1.

Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, P. R. China.

The iminoboryl -carboranes (MeSi)-Cb-B≡N-R (Cb = BCH, , R = SiMe; , R = Bu) have been successfully synthesized by tetrahydrofuran (THF)-promoted isomerization from the corresponding -carborane-fused aminoboriranes Cb{BN(SiMe)R} (). The synthetic protocol of the previously reported borirane was optimized. The borirane Cb{BN(SiMe)Bu} () and the iminoboranes and were fully characterized by NMR, IR, and single-crystal X-ray diffraction analyses. The borirane isomerizes more readily than . The kinetics study revealed a bimolecular mechanism between borirane and THF, which is in good agreement with the computationally proposed reaction pathway. The title compounds are thermally robust, but compound dimerized in the presence of a catalytic amount of BuNC to give the cyclodimer . Quick equilibrium between and the isonitrile adduct was observed in solution.
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http://dx.doi.org/10.1021/acs.inorgchem.2c00944DOI Listing
June 2022

Long chain triglyceride-lipid formulation promotes the oral absorption of the lipidic prodrugs through coincident intestinal behaviors.

Eur J Pharm Biopharm 2022 Jul 25;176:122-132. Epub 2022 May 25.

Department of Pharmaceutics, Wuya College of Innovation, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang 110016, China. Electronic address:

Oral administration of chemotherapy agents, such as docetaxel (DTX), is expected to reduce side effects significantly and increase dosing frequency. However, they often suffer from poor oral bioavailability, impeding their oral application. Dietary lipids such as triglycerides favor lymphatic transport nor vein system, bypassing the first-pass metabolism. Inspired by this concept, we developed a triglyceride-like prodrug of DTX (named as OATG) and explored the effect of lipid types on the OATG oral delivery. The plasma profile in rats revealed that long chain triglyceride (LCT)-based lipid formulations (LBLF) were more promising for OATG delivery than medium chain triglyceride (MCT) ones. The OATG LBLF elicited a markedly enhanced absorption compared with oral Taxotere or DTX LBLF, resulting in relative bioavailability 6.11 or 2.47-fold higher, respectively. The coincident intestinal behaviors of lipid excipients and TG-like prodrug facilitate the oral absorption of the prodrug. The effectiveness of the prodrug formulation was also examined in beagles with absolute bioavailability up to 41.08%, in sharp contrast to that of control DTX group (8%). Besides, the OATG oral formulation could be schedule-intensively administrated with no hypersensitivity, gastrointestinal and hematological toxicity. The current strategy provides an effective lipid formulation and a promising chance for chemotherapy at home.
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http://dx.doi.org/10.1016/j.ejpb.2022.05.015DOI Listing
July 2022

Thermal Insulation Performance of SiC-Doped Silica Aerogels under Large Temperature and Air Pressure Differences.

Gels 2022 May 20;8(5). Epub 2022 May 20.

College of Energy, Soochow University, 333 East Ganjiang Road, Suzhou 215031, China.

Silica aerogel composite is an excellent thermal insulator for spacecraft under high-temperature and complex air environments. This study intends to evaluate SiC-doped silica aerogel's thermal insulation performance under large temperature and air pressure differences. In this paper, the hot surface's temperature response of SiC-doped silica aerogel with different content was studied at significant temperature differences (Δ) when pressure changes instantaneously. Their thermal insulation performance was evaluated by analyzing the influence of pressure gradients on the unsteady-state heat transfer. When the cold surface's temperature of the specimen keeps constant at 15 °C and Δ = 171~912 K, the results demonstrate that the correlative thermal conductivities of silica aerogel with 1% and 5.84% SiC are 0.02223~0.04077 W·m·K at ≈ 10 Pa and 0.03165~0.04665 W·m·K at = 1 atm, respectively. The aerogel composite with 0% SiC showed the best thermal insulation performance at Δ < 200 K and ≈ 10 Pa, while the aerogel with 5.84% SiC became the best at Δ > 700 K and = 1 atm. In addition, the transient pressure decreases will significantly impair the heat transfer of the gas inside the aerogel, thereby weakening the gaseous thermal conductivity and improving the thermal insulation performance.
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http://dx.doi.org/10.3390/gels8050320DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141499PMC
May 2022

Efficient Linkable Ring Signature Scheme over NTRU Lattice with Unconditional Anonymity.

Comput Intell Neurosci 2022 13;2022:8431874. Epub 2022 May 13.

School of Software, Henan Polytechnic University, Jiaozuo 454000, China.

In cloud and edge computing, senders of data often want to be anonymous, while recipients of data always expect that the data come from a reliable sender and they are not redundant. Linkable ring signature (LRS) can not only protect the anonymity of the signer, but also detect whether two different signatures are signed by the same signer. Today, most lattice-based LRS schemes only satisfy computational anonymity. To the best of our knowledge, only the lattice-based LRS scheme proposed by Torres et al. can achieve unconditional anonymity. But the efficiency of signature generation and verification of the scheme is very low, and the signature length is also relatively long. With the preimage sampling, trapdoor generation, and rejection sampling algorithms, this study proposed an efficient LRS scheme with unconditional anonymity based on the e-NTRU problem under the random oracle model. We implemented our scheme and Torres et al.'s scheme, as well as other four efficient lattice-based LRS schemes. It is shown that under the same security level, compared with Torres et al.'s scheme, the signature generation time, signature verification time, and signature size of our scheme are reduced by about 94.52%, 97.18%, and 58.03%, respectively.
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http://dx.doi.org/10.1155/2022/8431874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122676PMC
May 2022
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