Publications by authors named "Qing Yan"

229 Publications

Fabrication of Robust, Highly Conductive, and Elastic Hybrid Carbon Foam Platform for High-Performance Compressible Asymmetry Supercapacitors.

ACS Omega 2021 Jun 25;6(22):14230-14241. Epub 2021 May 25.

College of Material Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, P. R. China.

Highly conductive and elastic three-dimensional (3D) porous carbon materials are ideal platforms to fabricate electrodes for high-performance compressible supercapacitors. Herein, a robust, highly conductive, and elastic carbon foam (CF) hybrid material is reported, which is fabricated by integrating cellulose nanofiber/multiwalled carbon nanotube (CNF/MWCNT) aerogel sheets with a melamine sponge (MS), followed by carbonization. The carbonized CNF/MWCNT aerogel sheets contribute to the high conductivity and specific surface area of the CF, and the 3D network-like skeleton derived from the carbonization of the MS enhances the elasticity and stability of the CF. More importantly, the CF possesses good scalability, allowing the introduction of electroactive materials such as polypyrrole (PPy) and FeO to fabricate high-performance compressible PPy-CF and FeO-CF electrodes. Moreover, an assembled PPy-CF//FeO-CF device shows reversible charging-discharging at a voltage of 1.6 V and demonstrates a high specific capacitance (172.5 F/g) and an outstanding energy density (59.9 W h/kg). The device exhibits capacitance retention rates reaching 98.3% and stable energy storage characteristics even under different degrees of compressive deformation. This study offers a scalable strategy for fabricating high-performance compressible supercapacitors, thereby providing a new means of satisfying the energy storage needs of portable electronic devices that are prone to deformation.
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http://dx.doi.org/10.1021/acsomega.1c00952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190803PMC
June 2021

Efficiency Analysis of New Rural Cooperative Medical System in China: Implications for the COVID-19 Era.

Front Psychol 2021 28;12:686954. Epub 2021 May 28.

School of Economics and Management, Hunan University of Science and Engineering, Yongzhou, China.

The sudden outbreak of coronavirus disease 2019 (COVID-19) has caused a huge impact on the Chinese residents' health and economic level. In the pandemic background, the country and its institutions have introduced pandemic-related insurance to stabilize the national situation. At this stage, insurance has played an increasingly important role in social life. With the popularization of insurance, the idea of buying insurance to avoid risk has gradually become popular among people. Among them, the New Rural Cooperative Medical System (NRCMS) has been farmers' common choice. The NRCMS, a mutual aid system created by farmers spontaneously in the country, plays a great role in guaranteeing farmers access to basic health services, alleviating poverty caused by disease and returning to poverty due to disease, and promoting poverty alleviation and rural revitalization. Given this backdrop, we study the efficiency of the NRCMS that can effectively promote poverty alleviation and rural revitalization and ensure the people's happy life. Implementing the Data Envelopment Analysis (DEA), we find that technological progress is one of the main factors influencing the efficiency of the NRCMS. Therefore, it is important to improve the technology for providing the efficiency of the NRCMS and promoting the happiness of the society.
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http://dx.doi.org/10.3389/fpsyg.2021.686954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193048PMC
May 2021

Anti-tumor activity of a novel proteasome inhibitor D395 against multiple myeloma and its lower cardiotoxicity compared with carfilzomib.

Cell Death Dis 2021 Apr 30;12(5):429. Epub 2021 Apr 30.

Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing, 210029, China.

Carfilzomib, a second-generation proteasome inhibitor, has significantly improved the survival rate of multiple myeloma (MM) patients, but its clinical application is still restricted by drug resistance and cardiotoxicity. Here, we identified a novel proteasome inhibitor, D395, and assessed its efficacy in treating MM as well as its cardiotoxicity at the preclinical level. The activities of purified and intracellular proteasomes were measured to determine the effect of D395 on the proteasome. CCK-8 and flow cytometry experiments were designed to evaluate the effects of D395 on cell growth and apoptosis. The effects of D395 and carfilzomib on serum enzyme activity, echocardiography features, cardiomyocyte morphology, and hERG channels were also compared. In our study, D395 was highly cytotoxic to MM cell lines and primary MM cells but not normal cells, and it was well tolerated in vivo. Similar to carfilzomib, D395 inhibited osteoclast differentiation in a dose-dependent manner. In particular, D395 exhibited lower cardiotoxicity than carfilzomib in all experiments. In conclusion, D395 is a novel irreversible proteasome inhibitor that has remarkable anti-MM activity and mild cardiotoxicity in vitro and in vivo.
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http://dx.doi.org/10.1038/s41419-021-03701-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087809PMC
April 2021

Umbilical cord mesenchymal stem cells enhance the therapeutic effect of imipenem by regulating myeloid-derived suppressor cells in septic mice.

Ann Transl Med 2021 Mar;9(5):404

School of Medicine, Southeast University, Nanjing, China.

Background: Umbilical cord mesenchymal stem cells (UC-MSCs), which possess potent immunomodulatory effects and low immunogenicity, are considered to be a promising stem cell-based therapy for sepsis. In the current study, we aimed to investigate whether the combined use of UC-MSCs and imipenem has a better effect than imipenem alone in treating ()-induced sepsis and to explore the mechanism by which UC-MSCs exert their therapeutic effect in septic mice.

Methods: We randomly divided mice into five groups with 10 mice in each group: the normal control group (control group), the sepsis group (vehicle group), the MSCs treatment group (MSCs group), the imipenem treatment group (imipenem group), and the imipenem plus MSCs treatment group (imipenem + MSCs group). We monitored the survival rate in each group every 12 h for 3 days. After observing the survival rate, another 50 mice were also randomly divided into five groups, and the mice were sacrificed after 24 h. Bacterial colonies from the blood and peritoneal lavage fluid were counted in a blinded manner. Organ injury was analyzed by hematoxylin and eosin (HE) staining. Frequencies of myeloid-derived suppressor cells (MDSCs) in the blood, spleen, and bone marrow (BM) were determined by flow cytometry. Plasma levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β, and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA).

Results: Compared with imipenem treatment, the co-administration of UC-MSCs and imipenem dramatically improved the survival rate, decreased the bacterial load, and ameliorated organ injury. Furthermore, UC-MSCs treatment, either alone or in combination with imipenem, significantly increased plasma levels of IL-10 and the percentage of MDSCs by inducing arginase-1 in septic mice.

Conclusions: Our results indicated that UC-MSCs protect mice against sepsis by acting on MDSCs. Combination therapy of UC-MSCs and imipenem may be a new approach for the future clinical treatment of sepsis.
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http://dx.doi.org/10.21037/atm-20-6371DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033360PMC
March 2021

Identification of Spodoptera frugiperda importin alphas that facilitate the nuclear import of Autographa californica multiple nucleopolyhedrovirus DNA polymerase.

Insect Mol Biol 2021 Apr 9. Epub 2021 Apr 9.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.

Proteins containing nuclear localization signals (NLSs) are actively transported into the nucleus via the classic importin-α/β-mediated pathway, and NLSs are recognized by members of the importin-α family. Most studies of insect importin-αs have focused on Drosophila to date, little is known about the importin-α proteins in Lepidoptera insects. In this study, we identified four putative importin-α homologues, Spodoptera frugiperda importin-α1 (SfIMA1), SfIMA2, SfIMA4 and SfIMA7, from Sf9 cells. Immunofluorescence analysis showed that SfIMA2, SfIMA4 and SfIMA7 localized to the nucleus, while SfIMA1 distributed in cytoplasm. Additionally, SfIMA4 and SfIMA7 were also detected in the nuclear membrane of Sf9 cells. SfIMA1, SfIMA4 and SfIMA7, but not SfIMA2, were found to associate with the C terminus of AcMNPV DNA polymerase (DNApol) that harbours a typical monopartite NLS and a classic bipartite NLS. Further analysis of protein-protein interactions revealed that SfIMA1 specifically recognizes the bipartite NLS, while SfIMA4 and SfIMA7 bind to both monopartite and bipartite NLSs. Together, our results suggested that SfIMA1, SfIMA4 and SfIMA7 play important roles in the nuclear import of AcMNPV DNApol C terminus in Sf9 cells.
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http://dx.doi.org/10.1111/imb.12704DOI Listing
April 2021

A Model-Based Meta-Analysis of Willingness to Participate in Cancer Screening.

Int J Environ Res Public Health 2021 03 4;18(5). Epub 2021 Mar 4.

School of Journalism and Communication, Jinan University, Guangzhou 510610, China.

Although early screening tests are beneficial for the detection and treatment of cancers, many people have failed to participate in screening tests. The present study aims to explore the theoretical underpinning of low participation in screening programs using the method of meta-analytic structural equation modeling. It was found that the health belief model is the most adopted theoretical framework. Moreover, the intended uptake of screening was positively predicted only by cues to action, health literacy, and perceived susceptibility. As a result, a health intention model, including the three significant variables, is proposed. The practical implications of the findings are that health communication campaigns should focus on enlightening and engaging the public through all necessary means to raise awareness and transfer knowledge in relation to screening procedures as well as cancers per se.
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http://dx.doi.org/10.3390/ijerph18052580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967393PMC
March 2021

The Impact of Childhood Left-Behind Experience on the Mental Health of Late Adolescents: Evidence from Chinese College Freshmen.

Int J Environ Res Public Health 2021 03 9;18(5). Epub 2021 Mar 9.

Institute for Social and Economic Research, Nanjing Audit University, Nanjing 211815, China.

A paucity of public service afforded to migrant workers often begets a wide range of social problems. In China, hundreds of millions of migrant worker parents have to leave children behind in their hometowns. This paper investigated the long-term effects of the childhood experience of being left behind on the mental well-being of late adolescents. Mandatory university personality inventory (UPI) surveys (involving psychosomatic problems such as anxiety, depression, and stress) were conducted at a university in Jiangsu, China, during 2014-2017. The study sample consisted of 15,804 first-year college students aged between 15 and 28 years. The PSM method and the OLS regression model were employed. Controlling for the confounding factors (gender, age, single-child status, hometown location, ethnicity, and economic status), our empirical investigation demonstrated that childhood left-behind experience significantly worsened the mental health of the study sample, increasing the measure of mental ill-being by 0.661 standard deviations ( < 0.01). Moreover, the effects were consistently significant in subsamples divided by gender, single-child status, and hometown location; and the effects were greater for females, single-child students, and urban residents.
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http://dx.doi.org/10.3390/ijerph18052778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967284PMC
March 2021

90% yield production of polymer nano-memristor for in-memory computing.

Nat Commun 2021 Mar 31;12(1):1984. Epub 2021 Mar 31.

Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.

Polymer memristors with light weight and mechanical flexibility are preeminent candidates for low-power edge computing paradigms. However, the structural inhomogeneity of most polymers usually leads to random resistive switching characteristics, which lowers the production yield and reliability of nanoscale devices. In this contribution, we report that by adopting the two-dimensional conjugation strategy, a record high 90% production yield of polymer memristors has been achieved with miniaturization and low power potentials. By constructing coplanar macromolecules with 2D conjugated thiophene derivatives to enhance the π-π stacking and crystallinity of the thin film, homogeneous switching takes place across the entire polymer layer, with fast responses in 32 ns, D2D variation down to 3.16% ~ 8.29%, production yield approaching 90%, and scalability into 100 nm scale with tiny power consumption of ~ 10 J/bit. The polymer memristor array is capable of acting as both the arithmetic-logic element and multiply-accumulate accelerator for neuromorphic computing tasks.
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http://dx.doi.org/10.1038/s41467-021-22243-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012610PMC
March 2021

Correction to: Quantifying soil NO emissions from soil and anaerobically digested swine manure, nitrification and denitrification using N isotope labeling method.

Environ Sci Pollut Res Int 2021 Mar 31. Epub 2021 Mar 31.

Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, 300191, China.

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http://dx.doi.org/10.1007/s11356-021-13656-5DOI Listing
March 2021

A novel cytogenetic method to image chromatin interactions at subkilobase resolution: Tn5 transposase-based fluorescence in situ hybridization.

J Genet Genomics 2020 Dec 7;47(12):727-735. Epub 2020 Nov 7.

Department of Automation, Tsinghua University, Beijing, 100084, China; MOE Key Laboratory of Bioinformatics, Bioinformatics Division, BNRist, Center for Synthetic & Systems Biology, Tsinghua University, Beijing, 100084, China. Electronic address:

There is an increasing interest in understanding how three-dimensional organization of the genome is regulated. Different strategies have been used to identify genome-wide chromatin interactions. However, owing to current limitations in resolving genomic contacts, visualization and validation of these genomic loci at subkilobase resolution remain unsolved to date. Here, we describe Tn5 transposase-based fluorescence in situ hybridization (Tn5-FISH), a polymerase chain reaction-based, cost-effective imaging method, which can colocalize the genomic loci at subkilobase resolution, dissect genome architecture, and verify chromatin interactions detected by chromatin configuration capture-derived methods. To validate this method, short-range interactions in the keratin-encoding gene (KRT) locus in the topologically associated domain were imaged by triple-color Tn5-FISH, indicating that Tn5-FISH is very useful to verify short-range chromatin interactions inside the contact domain and TAD. Therefore, Tn5-FISH can be a powerful molecular tool for clinical detection of cytogenetic changes in numerous genetic diseases such as cancers.
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http://dx.doi.org/10.1016/j.jgg.2020.04.008DOI Listing
December 2020

Detection of atmospheric temperature by using polarization high-spectral-resolution lidar.

Appl Opt 2021 Mar;60(8):2109-2117

In order to achieve high signal-to-noise ratio by using small laser energy and telescope aperture, we present a polarization filter in high-spectral-resolution lidar (HSRL) for the measurement of atmospheric temperature. Compared with the filter method in a traditional HSRL in which the intensity of the return signal is split into the different transmission channel of a discriminator, the advantage of this filter system is that the intensity of the return signal is fully utilized for each discriminator channel, and the return signal changes the polarization state of the light without loss of intensity when it is incident on the two Rayleigh channels. In addition, the daytime detection capability of HSRL is improved by using a polarization optical scheme to suppress the solar background light. The advantages of the polarization filter are proven by the theoretical calculations using the Stokes vector and a Mueller matrix. In detection experiments of atmospheric temperature, the detection height is 4 km at night and 2.5 km during the day by using the pulsed energy of 50 mJ and telescope diameter of 250 mm. The results are in good agreement with the data detected by radiosonde.
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http://dx.doi.org/10.1364/AO.412676DOI Listing
March 2021

Common and Specific Alterations of Amygdala Subregions in Major Depressive Disorder With and Without Anxiety: A Combined Structural and Resting-State Functional MRI Study.

Front Hum Neurosci 2021 15;15:634113. Epub 2021 Feb 15.

Department of Psychiatry, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Anxious major depressive disorder is a common subtype of major depressive disorder; however, its unique neural mechanism is not well-understood currently. Using multimodal MRI data, this study examined common and specific alterations of amygdala subregions between patients with and without anxiety. No alterations were observed in the gray matter volume or intra-region functional integration in either patient group. Compared with the controls, both patient groups showed decreased functional connectivity between the left superficial amygdala and the left putamen, and between the right superficial amygdala and the bilateral anterior cingulate cortex and medial orbitofrontal cortex, while only patients with anxiety exhibited decreased activity in the bilateral laterobasal and superficial amygdala. Moreover, the decreased activity correlated negatively with the Hamilton depression scale scores in the patients with anxiety. These findings provided insights into the pathophysiologic processes of anxious major depressive disorder and may help to develop new and effective treatment programs.
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http://dx.doi.org/10.3389/fnhum.2021.634113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917186PMC
February 2021

Stilbene glycoside upregulates SIRT3/AMPK to promotes neuronal mitochondrial autophagy and inhibit apoptosis in ischemic stroke.

Adv Clin Exp Med 2021 Feb;30(2):139-146

Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, China.

Background: Ischemic stroke, also known as cerebrovascular accident or cerebral stroke, occupies the first place in the world's top 10 causes of death, with high incidence, mortality and disability rates.

Objectives: To investigate the effect of stilbene glycoside upregulated SIRT3/AMPK expression on neuronal mitochondrial autophagy and neuronal apoptosis in ischemic stroke.

Material And Methods: The PC12 cells were cultured without serum to construct an ischemic neuron model. The cells were divided into 6 groups: normal group (untreated cells), model group (ischemic treated cells), TSG group (stilbene glycoside treatment), NC group (SIRT3 and AMPK negative control treatment), si-SIRT3 group (SIRT3 silencing treatment), TSG+si-SIRT3 group (joint treatment), and TSG+si-SIRT3+oe-AMPK group (joint treatment). Cell survival and the expression of related molecules were detected.

Results: Compared with normal group, the model group had significantly decreased cell survival rate, mitochondrial membrane potential, as well as the expression of Bcl-2, LC3II/I, P62, PINK1, Parkin, SIRT3, AMPK, and p-AMPK, while showing significantly increased proportion of apoptosis and the expression of caspase 3 and Bax. Compared with the model group, TSG treatment promoted cell survival rate and mitochondrial autophagy, and inhibited apoptosis, while SIRT3 silencing treatment reduced cell survival rate and mitochondrial autophagy, and increased apoptosis. The SIRT3 silencing could block the inhibitory effect of TSG on the apoptosis of ischemic PC12 cells and promote mitochondrial autophagy, and AMPK overexpression could save the apoptosis of ischemic PC12 cells caused by SIRT3 silencing, and promote mitochondrial autophagy.

Conclusions: By promoting the expression of SIRT3/AMPK, TSG promotes mitochondrial autophagy in ischemic neurons and inhibits their apoptosis.
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http://dx.doi.org/10.17219/acem/130608DOI Listing
February 2021

Quantifying soil NO emissions from soil and anaerobically digested swine manure, nitrification and denitrification using N isotope labeling method.

Environ Sci Pollut Res Int 2021 Feb 23. Epub 2021 Feb 23.

Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, 300191, China.

Increasing use of anaerobically digested swine manure in the farmland makes it necessary to understand its impact on NO emissions, regarding the source of NO and the corresponding mechanism of action. We used a N-labeled sulfate modifying the soil in order to identify the sources of NO and the pathways of nitrification and denitrification. Three soil moisture contents (50% WHC, 75% WHC, and 100% WHC) along with three levels of anaerobically digested swine manure (0 g·kg, 10 g·kg, and 25 g·kg) were tested using randomized block design. Although the combined effect of contents of anaerobically digested swine manure and the soil moisture contents added to the system stimulated the utilization of soil N and promoted denitrification, the process of nitrification dominated. In anaerobically digested swine manure-treated soils, the rate of contribution of anaerobically digested swine manure to NO accounted for 68.6-99.8%. In the 25 g·kg treatment, the maximum of NO produced by denitrification and nitrification were 14.1% and 93.1%.
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http://dx.doi.org/10.1007/s11356-021-12981-zDOI Listing
February 2021

Elevated preoptic brain activity in zebrafish glial glycine transporter mutants is linked to lethargy-like behaviors and delayed emergence from anesthesia.

Sci Rep 2021 Feb 4;11(1):3148. Epub 2021 Feb 4.

Department of Biology, University of Miami, 1301 Memorial Drive, Coral Gables, FL, 33146, USA.

Delayed emergence from anesthesia was previously reported in a case study of a child with Glycine Encephalopathy. To investigate the neural basis of this delayed emergence, we developed a zebrafish glial glycine transporter (glyt1 - / -) mutant model. We compared locomotor behaviors; dose-response curves for tricaine, ketamine, and 2,6-diisopropylphenol (propofol); time to emergence from these anesthetics; and time to emergence from propofol after craniotomy in glyt1-/- mutants and their siblings. To identify differentially active brain regions in glyt1-/- mutants, we used pERK immunohistochemistry as a proxy for brain-wide neuronal activity. We show that glyt1-/- mutants initiated normal bouts of movement less frequently indicating lethargy-like behaviors. Despite similar anesthesia dose-response curves, glyt1-/- mutants took over twice as long as their siblings to emerge from ketamine or propofol, mimicking findings from the human case study. Reducing glycine levels rescued timely emergence in glyt1-/- mutants, pointing to a causal role for elevated glycine. Brain-wide pERK staining showed elevated activity in hypnotic brain regions in glyt1-/- mutants under baseline conditions and a delay in sensorimotor integration during emergence from anesthesia. Our study links elevated activity in preoptic brain regions and reduced sensorimotor integration to lethargy-like behaviors and delayed emergence from propofol in glyt1-/- mutants.
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http://dx.doi.org/10.1038/s41598-021-82342-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862283PMC
February 2021

IL-37 Confers Anti-Tumor Activity by Regulation of m6A Methylation.

Front Oncol 2020 8;10:526866. Epub 2021 Jan 8.

Clinical Laboratory, Qingdao Central Hospital, The Second Affiliated Hospital of Medical College of Qingdao University, Qingdao, China.

N6-methyladenosine (m6A) is a common transcriptomic modification in cancer. Recently, it has been found to be involved in the regulation of non-small cell lung cancer (NSCLC) formation and metastasis. Interleukin 37 (IL-37) plays a crucial protective role in lung cancer. In our previous studies, we found that IL-37 is a potential novel tumor suppressor by inhibiting IL-6 expression to suppress STAT3 activation and decreasing epithelial-to-mesenchymal transition. Moreover, we found that treatment of IL-37 in lung cancer cells induced widespread and dynamic RNA m6A methylation. The effects of RNA m6A methylation of IL-37 treatment require further study. However, the functions of RNA m6A methylation of IL-37 treatment still await elucidation. Using MeRIP-seq and RNA-seq, we uncovered a unique m6A methylation profile in the treatment of IL-37 on the A549 cell line. We also showed the expression of m6A writers METTL3, METTL14, and WTAP and erasers ALKBH5 and FTO in A549 cells and lung cancer tissues after the treatment of IL-37. This study showed that IL-37 could lead to changes in m6A methylation level and related molecule expression level in A546 cells and may downregulate the proliferation by inhibiting Wnt5a/5b pathway in A549 cells. We conclude that IL-37 suppresses tumor growth through regulation of RNA m6A methylation in lung cancer cells.
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http://dx.doi.org/10.3389/fonc.2020.526866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821743PMC
January 2021

Evaluation of fronto-orbital reconstruction surgery for the treatment of metopic synostosis in Chinese population.

Childs Nerv Syst 2021 Apr 6;37(4):1167-1174. Epub 2021 Jan 6.

Department of Neurosurgery, Children's Hospital of Nanjing Medical University, Nanjing, China.

Purpose: To evaluate the efficacy of fronto-orbit reconstruction surgery on pediatric metopic synostosis via an image-based 3D reconstruction in Chinese population.

Methods: Thirty pediatric metopic synostosis patients who received fronto-orbital reconstruction surgery in the Children's Hospital of Nanjing Medical University, Department of Neurosurgery, from January 2007 to December 2018 were analyzed in the study. Here we use the Mimics 20.0 software to reconstruct patients' cranial thin-section CT scan images from pre- and post-operation and control groups. Then the data of intracranial volume, frontal volume, orbital hypertelorism, ECA, ZF, and ORA were analyzed using the paired t-test or Wilcoxon matched-pairs signed-ranks test.

Results: The age of these patients was 15.83 ± 16.12 months. After surgery, the mean frontal volume was enlarged from 92.75 ± 26.97 to 138.62 ± 47.97 cm (P < 0.0001), and the intracranial volume was enhanced from 976.87 ± 230.83 to 1059.44 ± 217.98 cm (P < 0.0001). In the meantime, the ECA was changed from 108.02 ± 8.17 to 134 ± 5.59° (P < 0.0001). In line with the alteration of the parameters mentioned above, the head shapes in all patients were also significantly improved after the surgery with no obvious complications.

Conclusion: Fronto-orbit reconstruction surgery is a safe and effective treatment for pediatric metopic synostosis. Computer-aided 3D reconstruction could serve as a quantitative strategy to evaluate the efficacy of craniofacial surgery.
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http://dx.doi.org/10.1007/s00381-020-04977-wDOI Listing
April 2021

Onion extract gel is not better than other topical treatments in scar management: A meta-analysis from randomised controlled trails.

Int Wound J 2021 Jun 28;18(3):396-409. Epub 2020 Dec 28.

Department of Plastic Surgery, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University), Chongqing, China.

To evaluate the efficacy and safety of onion extract (OE) gel on scar management, a systematic review was performed by searching Embase, PubMed, Medline, and the Cochrane Library databases, and a meta-analysis was conducted according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement guidelines. Finally, 13 randomised controlled trails were enrolled for meta-analysis. OE gel increased the total improvement scores assessed by investigators (P < .00001) and patients (P < .00001) than no treatment, but no differences were detected between OE gel and other commonly used topical treatments assessed by investigators (P = .56) and patients (P = .39). Moreover, OE in silicone gel increased the total improvement scores assessed by investigators (P < .00001) and patients (P = .0007) than other treatments. OE gel increased the incidence of total adverse effects compared with no treatment (P < .0001) and other treatments (P = .008) by a fixed-effects model, and increased the incidence of dropping out caused by intolerance of treatments (P = .0002). OE gel not only has no superiority to commonly used topical treatments, but also has the potential to increase the incidence of adverse effects on scar management; OE in silicone gel might be the optimal topical choice for scar treatment; however, more evidences are needed to strength these conclusions.
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http://dx.doi.org/10.1111/iwj.13542DOI Listing
June 2021

ABCG1 Attenuates Oxidative Stress Induced by HO through the Inhibition of NADPH Oxidase and the Upregulation of Nrf2-Mediated Antioxidant Defense in Endothelial Cells.

Anal Cell Pathol (Amst) 2020 3;2020:2095645. Epub 2020 Dec 3.

Department of Cardiovascular Medicine, Second Affiliated Hospital of Xi'an Jiaotong University, 157 West Five Road, Xi'an, Shaanxi 710004, China.

. Oxidative stress is an important factor that is related to endothelial dysfunction. ATP-binding cassette transporter G1 (ABCG1), a regulator of intracellular cholesterol efflux, has been found to prevent endothelial activation in vessel walls. To explore the role of ABCG1 in oxidative stress production in endothelial cells, HUAECs were exposed to HO and transfected with the specific ABCG1 siRNA or ABCG1 overexpression plasmid. The results showed that overexpression of ABCG1 by ABCG1 plasmid or liver X receptor (LXR) agonist T0901317 treatment inhibited ROS production and MDA content induced by HO in HUAECs. Furthermore, ABCG1 upregulation blunted the activity of prooxidant NADPH oxidase and the expression of Nox4, one of the NADPH oxidase subunits. Moreover, the increased migration of Nrf2 from the cytoplasm to the nucleus and antioxidant HO-1 expression were detected in HUAECs with upregulation of ABCG1. Conversely, ABCG1 downregulation by ABCG1 siRNA increased NADPH oxidase activity and Nox4 expression and abrogated the increase at Nrf2 nuclear protein levels. In addition, intracellular cholesterol load interfered with the balance between NADPH oxidase activity and HO-1 expression. It was suggested that ABCG1 attenuated oxidative stress induced by HO in endothelial cells, which might be involved in the balance between decreased NADPH oxidase activity and increased Nrf2/OH-1 antioxidant defense signaling via its regulation for intracellular cholesterol accumulation.
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http://dx.doi.org/10.1155/2020/2095645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7732382PMC
December 2020

Irrigation with secondary municipal-treated wastewater: Potential effects, accumulation of typical antibiotics and grain quality responses in rice (Oryza sativa L.).

J Hazard Mater 2021 05 25;410:124655. Epub 2020 Nov 25.

China National Rice Research Institute, Hangzhou, China; Laboratory of Quality & Safety Risk Assessment for Rice (Hangzhou), Ministry of Agriculture, Hangzhou 310006, China. Electronic address:

Using secondary treated wastewater to irrigate paddies presents an exposure pathway for antibiotics to enter the terrestrial food chain. To date, there has been no information on the biochemical reactions and antibiotic uptake in rice plants irrigated with secondary treated wastewater. The present study investigated antibiotic uptake and concentration-response trends in rice tissues and evaluated the effects of typical antibiotics (tetracycline, roxithromycin, ofloxacin, and sulfamethoxazole) on rice growth, grain yield and quality, and rice physiobiochemical characters via irrigation using treated wastewater augmented with varying concentrations (0-500 µg/L) in paddies. The results showed that the antibiotic accumulation in rice plants irrigated with treated wastewater was limited, and the studied antibiotics were not detected in rice grains (edible parts). The ability of rice to withstand certain antibiotics and grow in a healthy manner is attributed to the capacity to maintain reasonably normal photosynthesis activity and to elevate antioxidative defenses. The highest antibiotic concentration (500 µg/L) did not reduce the processing quality of the rice grain, but it enhanced the cooking and eating quality. From the obtained results, it can be concluded that secondary treated wastewater for paddy irrigation is an alternative water resource securing protection from the environment and rice grain quality.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124655DOI Listing
May 2021

Protective effects of HBOC on pulmonary vascular leakage after haemorrhagic shock and the underlying mechanisms.

Artif Cells Nanomed Biotechnol 2020 Dec;48(1):1272-1281

State Key Laboratory of Trauma, Burns and Combined Injury, Second Department of Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing, P.R. China.

Volume resuscitation is an important early treatment for haemorrhagic shock. Haemoglobin-based oxygen carrier (HBOC) can expand the volume and provide oxygen for tissues. Vascular leakage is common complication in the process of haemorrhagic shock and resuscitation. The aim of this study was to observe the effects of HBOC (a bovine-derived, cross-linked tetramer haemoglobin oxygen-carrying solution, 0.5 g/L) on vascular leakage in rats after haemorrhagic shock. A haemorrhagic shock rat model and hypoxic vascular endothelial cells (VECs) were used. The role of intercellular junctions and endothelial glycocalyx in the protective effects of HBOC and the relationship with mitochondrial function were analysed. After haemorrhagic shock, the pulmonary vascular permeability to FITC-BSA, Evans Blue was increased, endothelial glycocalyx was destroyed and the expression of intercellular junction proteins was decreased. After haemorrhagic shock, a small volume of HBOC solution (6 ml/kg) protected pulmonary vascular permeability, increased structural thickness of endothelial glycocalyx, the levels of its components and increased expression levels of the intercellular junction proteins ZO-1, VE-cadherin and occludin. Moreover, HBOC significantly increased oxygen delivery and consumption in rats, improved VEC mitochondrial function and structure. In conclusion, HBOC mitigates endothelial leakage by protecting endothelial glycocalyx and intercellular junctions through improving mitochondrial function and tissue oxygen delivery.
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http://dx.doi.org/10.1080/21691401.2020.1835937DOI Listing
December 2020

Cyclic-di-GMP Regulates the Quorum-Sensing System and Biocontrol Activity of Pseudomonas fluorescens 2P24 through the RsmA and RsmE Proteins.

Appl Environ Microbiol 2020 11 24;86(24). Epub 2020 Nov 24.

College of Agriculture, Guangxi University, Nanning, China

2P24 is a rhizosphere bacterium that protects many crop plants against soilborne diseases caused by phytopathogens. The PcoI/PcoR quorum-sensing (QS) system and polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) are particularly relevant to the strain's biocontrol potential. In this study, we investigated the effects of c-di-GMP on the biocontrol activity of strain 2P24. The expression of the diguanylate cyclase (YedQ) and phosphodiesterase (YhjH) in 2P24 significantly increased and decreased the cellular concentration of c-di-GMP, respectively. The production of the QS signals -acyl homoserine lactones (AHLs) and 2,4-DAPG was negatively regulated by c-di-GMP in 2P24. The regulatory proteins RsmA and RsmE were positively regulated by c-di-GMP. Genomic analysis revealed that 2P24 has 23 predicted proteins that contain c-di-GMP-synthesizing or -degrading domains. Among these proteins, C0J56_12915, C0J56_13325, and C0J56_27925 contributed to the production of c-di-GMP and were also involved in the regulation of the QS signal and antibiotic 2,4-DAPG production in Overexpression of C0J56_12915, C0J56_13325, and C0J56_27925 in 2P24 impaired its root colonization and biocontrol activities. Taken together, these results demonstrated that c-di-GMP played an important role in fine-tuning the biocontrol traits of In various bacteria, the bacterial second messenger c-di-GMP influences a wide range of cellular processes. However, the function of c-di-GMP on biocontrol traits in the plant-beneficial rhizobacteria remains largely unclear. The present work shows that the QS system and polyketide antibiotic 2,4-DAPG production are regulated by c-di-GMP through RsmA and RsmE proteins in 2P24. The diguanylate cyclases (DGCs) C0J56_12915, C0J56_13325, and C0J56_27925 are especially involved in regulating the biocontrol traits of 2P24. Our work also demonstrated a connection between the Gac/Rsm cascade and the c-di-GMP signaling pathway in .
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http://dx.doi.org/10.1128/AEM.02016-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688223PMC
November 2020

Total Cranial Reconstruction for the Treatment of Sagittal Craniosynostosis in Children.

J Craniofac Surg 2021 Jan-Feb 01;32(1):218-223

Department of Neurosurgery, Children's Hospital of Nanjing Medical University, Nanjing, China.

Objective: To study the effect of total cranial reconstruction for sagittal synostosis (scaphocephaly) deformity in Chinese children.

Methods: A retrospective analysis was performed involving 23 children with isolated non-syndromic sagittal synostosis who were treated by total calvarial vault remodeling after 1 year of age from May 2015 to June 2019 in the Department of Neurosurgery, Children's Hospital of Nanjing Medical University. The authors reconstruct patients' pre- and post-operative cranial thin-section CT scan images and those of the control group. The cephalic index (traditional, normative), intracranial volume, horizontal point of maximum width (H-PMW), vertical point of maximum width (V-PMW), frontal to head height ratio and occipital to head height ratio data were analyzed using a paired t test or Wilcoxon signed-rank test.

Results: Twenty-three patients met the inclusion criteria, including 19 males and 4 females. The ratio of males to females was 4.7:1. All patients underwent total cranial reconstruction. The average age was 26.52 months (13-48 months), the average operation time was 214.13 minutes (150-265 minutes), and the average amount of suspended erythrocytes was 200 ml (100-400 ml). The cranial morphology of all patients improved significantly after the operation. The traditional cephalic index (pre-operative: 0.70 (0.04); post-operative: 0.78 (0.02)) and normative cephalic index (pre-operative: 0.68 (0.03); post-operative: 0.77 (0.02)) were significantly increased (P < 0.0001). The mean horizontal point of maximum width improved from 0.54 to 0.56 (P = 0.0043), the mean vertical point of maximum width decreased from 0.59 to 0.54 (P = 0.0006), the frontal height decreased from 0.89 to 0.77 (P < 0.0001), and the occipital height improved from 0.78 to 0.88 (P < 0.0001). The intracranial volume increased from 1287.35 to 1426.90 cm3 (P < 0.0001). All of the children had a good skull shape and no recurrence of deformity.

Conclusions: Total calvarial reconstruction can effectively correct scaphocephaly in Chinese children, expand cranial volume, reduce cranial height, shorten fronto-occipital diameters and enlarge biparietal diameters.
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http://dx.doi.org/10.1097/SCS.0000000000006977DOI Listing
April 2021

The Effect of SiMe and SiEt Para Substituents for High Activity and Introduction of a Hydroxy Group in Ethylene Copolymerization Catalyzed by Phenoxide-Modified Half-Titanocenes.

Angew Chem Int Ed Engl 2020 Dec 7;59(51):23072-23076. Epub 2020 Oct 7.

Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami Osawa, Hachioji, Tokyo, 192-0376, Japan.

Remarkable effects of SiMe and SiEt para-substituents in the phenoxide-modified half-titanocenes, Cp*TiCl (O-2,6- Pr -4-R-C H ) [R=SiMe (6), SiEt (7)], toward the catalytic activities in ethylene copolymerizations with 2-methyl-1-pentene, 1-decene, 1-dodecene and with 9-decen-1-ol (DC-OH) have been demonstrated. The activities by 6, 7 at 50 °C showed higher than those conducted at 25 °C in all cases in the presence of MAO cocatalyst. Efficient synthesis of high-molecular-weight (HMW) ethylene copolymers incorporating DC-OH (or 5-hexen-1-ol, HX-OH) has been attained in the copolymerization by 7, which showed better DC-OH (HX-OH) incorporation at 50 °C to afford the HMW copolymers, poly(ethylene-co-DC-OH)s, with high activities (activity 1.21-3.81×10  kg-polymer mol -Ti h, M =6.55-10.0×10 , DC-OH 2.3-3.6 mol %).
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http://dx.doi.org/10.1002/anie.202010559DOI Listing
December 2020

Combination of apolipoprotein-A-I/apolipoprotein-A-I binding protein and anti-VEGF treatment overcomes anti-VEGF resistance in choroidal neovascularization in mice.

Commun Biol 2020 07 16;3(1):386. Epub 2020 Jul 16.

Cullen Eye Institute, Baylor College of Medicine, Houston, TX, 77030, USA.

Many patients of choroidal neovascularization (CNV) are unresponsive to the current anti-VEGF treatment. The mechanisms for anti-VEGF resistance are poorly understood. We explore the unique property of the apolipoprotein A-I (apoA-I) binding protein (AIBP) that enhances cholesterol efflux from endothelial cells and macrophages to thereby limit angiogenesis and inflammation to tackle anti-VEGF resistance in CNV. We show that laser-induced CNV in mice with increased age showed increased resistance to anti-VEGF treatment, which correlates with increased lipid accumulation in macrophages. The combination of AIBP/apoA-I and anti-VEGF treatment overcomes anti-VEGF resistance and effectively suppresses CNV. Furthermore, macrophage depletion in old mice restores CNV sensitivity to anti-VEGF treatment and blunts the synergistic effect of combination therapy. These results suggest that cholesterol-laden macrophages play a critical role in inducing anti-VEGF resistance in CNV. Combination therapy by neutralizing VEGF and enhancing cholesterol removal from macrophages is a promising strategy to combat anti-VEGF resistance in CNV.
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http://dx.doi.org/10.1038/s42003-020-1113-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367303PMC
July 2020

Ancient co-option of an amino acid ABC transporter locus in Pseudomonas syringae for host signal-dependent virulence gene regulation.

PLoS Pathog 2020 07 16;16(7):e1008680. Epub 2020 Jul 16.

Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, United States of America.

Pathogenic bacteria frequently acquire virulence traits via horizontal gene transfer, yet additional evolutionary innovations may be necessary to integrate newly acquired genes into existing regulatory pathways. The plant bacterial pathogen Pseudomonas syringae relies on a horizontally acquired type III secretion system (T3SS) to cause disease. T3SS-encoding genes are induced by plant-derived metabolites, yet how this regulation occurs, and how it evolved, is poorly understood. Here we report that the two-component system AauS-AauR and substrate-binding protein AatJ, proteins encoded by an acidic amino acid-transport (aat) and -utilization (aau) locus in P. syringae, directly regulate T3SS-encoding genes in response to host aspartate and glutamate signals. Mutants of P. syringae strain DC3000 lacking aauS, aauR or aatJ expressed lower levels of T3SS genes in response to aspartate and glutamate, and had decreased T3SS deployment and virulence during infection of Arabidopsis. We identified an AauR-binding motif (Rbm) upstream of genes encoding T3SS regulators HrpR and HrpS, and demonstrated that this Rbm is required for maximal T3SS deployment and virulence of DC3000. The Rbm upstream of hrpRS is conserved in all P. syringae strains with a canonical T3SS, suggesting AauR regulation of hrpRS is ancient. Consistent with a model of conserved function, an aauR deletion mutant of P. syringae strain B728a, a bean pathogen, had decreased T3SS expression and growth in host plants. Together, our data suggest that, upon acquisition of T3SS-encoding genes, a strain ancestral to P. syringae co-opted an existing AatJ-AauS-AauR pathway to regulate T3SS deployment in response to specific host metabolite signals.
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http://dx.doi.org/10.1371/journal.ppat.1008680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386598PMC
July 2020

Pleiotropic effects of RsmA and RsmE proteins in Pseudomonas fluorescens 2P24.

BMC Microbiol 2020 07 2;20(1):191. Epub 2020 Jul 2.

College of Plant Protection, China Agricultural University, Beijing, 100193, China.

Background: Pseudomonas fluorescens 2P24 is a rhizosphere bacterium that produces 2,4-diacetyphloroglucinol (2,4-DAPG) as the decisive secondary metabolite to suppress soilborne plant diseases. The biosynthesis of 2,4-DAPG is strictly regulated by the RsmA family proteins RsmA and RsmE. However, mutation of both of rsmA and rsmE genes results in reduced bacterial growth.

Results: In this study, we showed that overproduction of 2,4-DAPG in the rsmA rsmE double mutant influenced the growth of strain 2P24. This delay of growth could be partially reversal when the phlD gene was deleted or overexpression of the phlG gene encoding the 2,4-DAPG hydrolase in the rsmA rsmE double mutant. RNA-seq analysis of the rsmA rsmE double mutant revealed that a substantial portion of the P. fluorescens genome was regulated by RsmA family proteins. These genes are involved in the regulation of 2,4-DAPG production, cell motility, carbon metabolism, and type six secretion system.

Conclusions: These results suggest that RsmA and RsmE are the important regulators of genes involved in the plant-associated strain 2P24 ecologic fitness and operate a sophisticated mechanism for fine-tuning the concentration of 2,4-DAPG in the cells.
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http://dx.doi.org/10.1186/s12866-020-01880-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331252PMC
July 2020

Configuring hierarchical Ni/NiO 3D-network assisted with bamboo cellulose nanofibers for high-performance Ni-Zn aqueous batteries.

Nanoscale 2020 Jul 2;12(27):14651-14660. Epub 2020 Jul 2.

School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, Hunan, China.

The further application of Ni-Zn aqueous batteries is majorly restricted by nickel-based cathodes due to their low capacity and poor cycling stability, which requires the development of hierarchically nanostructured nickel and nickel oxides. Herein, we prepare a novel nickel-based electrode with hierarchical 3D networks by configuring nanostructured Ni and Ni/NiO nanoparticles onto bamboo-derived cellulose nanofibers (denoted as Ni/NiO-BCF). Owing to the high conductivity of carbonized nanofibers and enhanced Ni/NiO active sites exposed, the Ni/NiO-BCF electrode delivers a capacity of 248 mA h g at 0.625 A g and exhibits a good cycling stability (94.5% after 2000 cycles). The as-fabricated Ni/NiO-BCF//Zn battery shows a high capacity of 296 mA h g at 0.625 A g and excellent cycling stability (almost no decay after 1000 cycles). Notably, a peak energy density of 313.4 W h kg is also achieved from the Ni/NiO-BCF//Zn battery. This work provides novel insights into developing elaborately-nanostructured electrodes from natural and sustainable resources for high-capacity and long-cycle energy storage systems.
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http://dx.doi.org/10.1039/d0nr03608kDOI Listing
July 2020

Changes in colistin resistance and mcr-1 abundance in Escherichia coli of animal and human origins following the ban of colistin-positive additives in China: an epidemiological comparative study.

Lancet Infect Dis 2020 10 4;20(10):1161-1171. Epub 2020 Jun 4.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China.

Background: Following the discovery and emergence of the plasmid-mediated colistin resistance gene, mcr-1, the Chinese government formally banned colistin as an animal growth promoter on April 30, 2017. Herein, we report patterns in colistin resistance and mcr-1 abundance in Escherichia coli from animals and humans between 2015 and 2019, to evaluate the effects of the colistin withdrawal.

Methods: We did an epidemiology comparative study to investigate: annual production and sales of colistin in agriculture across mainland China according to data from the China Veterinary Drug Association from 2015 to 2018; the prevalence of colistin-resistant E coli (CREC) in pigs and chickens in 23 Chinese provinces and municipalities as reported in the China Surveillance on Antimicrobial Resistance of Animal Origin database from Jan 1, 2015, to Dec 31, 2016, and Jan 1, 2017, to Dec 31, 2018; the presence of residual colistin and mcr-1 in faeces from 118 animal farms (60 pig, 29 chicken, and 29 cattle) across four provinces over July 1, 2017, to August 31, 2017, and July 1, 2018 to August 31, 2018; the prevalence of mcr-1-positive E coli (MCRPEC) carriage in healthy individuals attending routine hospital examinations across 24 provinces and municipalities from June 1 to July 30, 2019, comparing with equivalent 2016 data (June 1 to September 30) from our previous study in the same hospitals; and the patterns in CREC prevalence among hospital E coli infections across 26 provinces and municipalities from Jan 1, 2015, to Dec 31, 2016, and Jan 1, 2018, to Dec 31, 2019, reported on the China Antimicrobial Surveillance Network.

Findings: After the ban on colistin as a growth promoter, marked reductions were observed in the production (27 170 tonnes in 2015 vs 2497 tonnes in 2018) and sale (US$71·5 million in 2015 vs US$8·0 million in 2018) of colistin sulfate premix. Across 118 farms in four provinces, mean colistin residue concentration was 191·1 μg/kg (SD 934·1) in 2017 versus 7·5 μg/kg (50·0) in 2018 (p<0·0001), and the median relative abundance of mcr-1 per 16S RNA was 0·0009 [IQR 0·0001-0·0059] in 2017 versus 0·0002 [0·0000-0·0020] in 2018 (p=0·0001). Across 23 provinces and municipalities, CREC was identified in pig faeces in 1153 (34·0%) of 3396 samples in 2015-16 versus 142 (5·1%) of 2781 in 2017-18 (p<0·0001); and in chickens in 474 (18·1%) of 2614 samples in 2015-16 versus 143 (5·0%) of 2887 in 2017-18 (p<0·0001). In hospitals across 24 provincial capital cities and municipalities, human carriage of MCRPEC was identified in 644 (14·3%) of 4498 samples in 2016 versus 357 (6·3%) of 5657 in 2019 (p<0·0001). Clinical CREC infections in 26 provinces and municipalities comprised 1059 (1·7%) of 62 737 E coli infections in 2015-16 versus 794 (1·3%) of 59 385 in 2018-19 (p<0·0001).

Interpretation: The colistin withdrawal policy and the decreasing use of colistin in agriculture have had a significant effect on reducing colistin resistance in both animals and humans in China. However, continuous colistin monitoring is essential, in particular to act as an early warning system for colistin stewardship in Chinese hospitals.

Funding: National Key Research and Development Program of China, National Natural Science Foundation of China, and UK Medical Research Council.
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http://dx.doi.org/10.1016/S1473-3099(20)30149-3DOI Listing
October 2020

A Chromosome-Scale Assembly of the Asian Honeybee Genome.

Front Genet 2020 27;11:279. Epub 2020 Mar 27.

Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, China.

is one of the main honeybee species in artificial farming, which is widely distributed in Asian countries. The genome of has been sequenced by several different research groups using second generation sequencing technologies. However, it is still necessary to obtain more complete and accurate genome sequences. Here we present a chromosome-scale assembly of the genome using single-molecule real-time (SMRT) Pacific Biosciences sequencing and high-throughput chromatin conformation capture (Hi-C) genome scaffolding. The updated assembly is 215.67 Mb in size with a contig N50 of 4.49 Mb, representing an 212-fold improvement over the previous Illumina-based version. Hi-C scaffolding resulted in 16 pseudochromosomes occupying 97.85% of the assembled genome sequences. A total of 10,741 protein-coding genes were predicted and 9,627 genes were annotated. Besides, 314 new genes were identified compared to the previous version. The improved high-quality reference genome will provide precise sequence information for biological research of .
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http://dx.doi.org/10.3389/fgene.2020.00279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119468PMC
March 2020