Publications by authors named "Qing X Li"

260 Publications

Isoorientin Affects Markers of Alzheimer's Disease via Effects on the Oral and Gut Microbiota in APP/PS1 Mice.

J Nutr 2021 Oct 12. Epub 2021 Oct 12.

Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Background: There is growing evidence of strong associations between the pathogenesis of Alzheimer's disease (AD) and dysbiotic oral and gut microbiota. Recent studies demonstrated that isoorientin (ISO) is anti-inflammatory and alleviates markers of AD, which were hypothesized to be mediated by the oral and gut microbiota.

Objectives: We studied the effects of oral administration of ISO on AD-related markers and the oral and gut microbiota in mice.

Methods: Eight-month-old amyloid precursor protein/presenilin-1 (AP) transgenic male mice were randomly allocated to 3 groups of 15 mice each: vehicle (AP) alone or with a low dose of ISO (AP + ISO-L; 25 mg/kg) or a high dose of ISO (AP + ISO-H; 50 mg/kg). Age-matched wild-type (WT) C57BL/6 male littermates were used as controls. The 4 groups were treated intragastrically with ISO or sterilized ultrapure water for 2 months. AD-related markers in the brain, serum, colon, and liver were analyzed with immunohistochemical and histochemical staining, Western blotting, and ELISA. Oral and gut microbiotas were analyzed using 16S ribosomal RNA gene sequencing.

Results: The high-dose ISO treatment significantly decreased amyloid beta 42-positive deposition by 38.1% and 45.2% in the cortex and hippocampus, respectively, of AP mice (P < 0.05). Compared with the AP group, both ISO treatments reduced brain phospho-Tau, phosphor-p65, phosphor-inhibitor of NF-κB, and brain and serum LPS and TNF-α by 17.9%-72.5% and increased brain and serum IL-4 and IL-10 by 130%-210% in the AP + ISO-L and AP + ISO-H groups (P < 0.05). Abundances of 26, 25, and 23 microbial taxa in oral, fecal and cecal samples, respectively, were increased in both the AP + ISO-L and AP + ISO-H groups relative to the AP group [linear discriminant analysis (LDA) >3.0; P < 0.05]. Gram-negative bacteria, Alteromonas, Campylobacterales, and uncultured Bacteroidales bacterium were positively correlated (rho = 0.28-0.59; P < 0.05) with the LPS levels and responses of inflammatory cytokines.

Conclusions: The microbiota-gut-brain axis is a potential mechanism by which ISO reduces AD-related markers in AP mice.
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http://dx.doi.org/10.1093/jn/nxab328DOI Listing
October 2021

Enantiomer metabolism of acephate and its metabolite methamidophos in in vitro tea (Camellia sinensis L.) systems: Comparison between cell suspensions and excised tissues.

Sci Total Environ 2021 Oct 6:150863. Epub 2021 Oct 6.

School of Resource & Environment, Anhui Agricultural University, Key Laboratory of Agri-food Safety of Anhui Province, Hefei 230036, PR China. Electronic address:

Enantioselective metabolism of chiral pesticide in plants is very important. In vitro system has become an effective means to study the metabolism of pesticides in plants, but the study on the metabolism of chiral pesticides has not been reported. This work compared the enantiomer metabolic behavior of acephate and its metabolite methamidophos between tea cell suspensions and excised tea stem with leaves. (±)-Acephate could be absorbed and transferred well to top leaves by the cut end of excised stem after 24 h. (±)-Methamidophos was derived from the metabolism of (±)-acephate in tea plants at 3-5% in leaves and 2-3% in stems at 216 h. The content of (+)-methamidophos was 1.5 times higher than that of (-)-methamidophos in excised leaves. Though both (±)-acephate and (±)-methamidophos could be metabolized well by cell suspension, (±)-acephate and (±)-methamidophos was non-enantioselectively metabolized in cell suspension. It was shown that using the excised tea stem with leaves for chiral pesticide metabolism studies was much closer to intact plant than cell suspensions. This result also established an effective and easily available in vitro metabolic model for the study of enantioselective metabolism of chiral contaminants from environment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150863DOI Listing
October 2021

Interactions of isoorientin and its Semi-synthetic analogs with human serum albumin.

Bioorg Chem 2021 Sep 1;116:105319. Epub 2021 Sep 1.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East-West Road, Honolulu, HI 96822, USA.

Isoorientin is a C-glycosyl flavone with a wide range of health beneficial effects and inhibits glycogen synthase kinase 3β (GSK-3β) potentially against Alzheimer's disease. Its semi-synthetic derivatives have greater potency than isoorientin. The present study was aimed to determine the mechanism of interactions of isoorientin and its derivatives with human serum albumin (HSA) using multi-spectroscopic, microscale thermophoresis (MST) and computational studies. Spectra of steady-state fluorescence, UV-Vis, and time-resolved fluorescence indicated that isoorientin and its derivatives quenched the intrinsic fluorescence of HSA through a static quenching process. Isoorientin and its derivatives had a moderate affinity with HSA (K 7.7-14.9 × 10 M). The binding process was accompanied by an exothermic phenomenon, ΔG° of HSA-isoorientin and its derivatives systems were calculated as from -29.51 kJ mol to -27.87 kJ mol. Displacement experiments with site-specific markers revealed that isoorientin and its derivatives bind to HSA at site II (subdomain IIIA) only. A reduction in the α-helical content of HSA-isoorientin and its derivatives complex was observed, because the conformational changes was structurally perturbed by the hydrophilic groups of the compounds. Further molecular modeling studies confirmed that the binding of isoorientin and its derivatives to the site II via hydrophobic interaction. The MST results confirmed the interactions between HSA and the compounds of interest. The esterase-like assay studies indicated that isoorientin and its derivatives shared the same binding site in HSA, and their induced structural changes of HSA may have been caused by partial unfolding of HSA. This work helps to understand transport, distribution, bioactivity, and design of flavonoid-based GSK-3β inhibitors.
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http://dx.doi.org/10.1016/j.bioorg.2021.105319DOI Listing
September 2021

Protecting and Enhancing Scarce Water Resources through Chemistry.

J Agric Food Chem 2021 08 6;69(32):9199-9201. Epub 2021 Aug 6.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, Hawaii 96822, United States.

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http://dx.doi.org/10.1021/acs.jafc.1c03540DOI Listing
August 2021

Chiral enantiomers of the plant growth regulator paclobutrazol selectively affect community structure and diversity of soil microorganisms.

Sci Total Environ 2021 Nov 16;797:148942. Epub 2021 Jul 16.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.

Paclobutrazol is a triazole plant growth regulator with a wide range of applications in crop and fruit tree production. Paclobutrazol is used as a racemic mixture in agriculture. However, the effects of paclobutrazol enantiomers on soil microbial community structure and diversity are unclear. In the present study, Illumina high-throughput sequencing was used to study the enantioselective effects of two paclobutrazol enantiomers on soil microbial community. S-paclobutrazol was more persistent than R-paclobutrazol. The half-lives of the S- and R-isomers were 80 d and 50 d, respectively. No interconversion between the two isomers occurred in soils. In addition, the enantiomers had significant enantiomeric effects on soil microbial community and the paclobutrazol degradation was probably attributed to the presence of Pseudomonas and Mycobacterium. Notably, the relative abundance of Fusarium, a genus of filamentous fungi producing gibberellins, could be enantioselectively affected by the chiral enantiomers. Paclobutrazol enantiomers exhibited greater effects on the fungal community structure than bacterial community structure due to the fungicidal activity of paclobutrazol. Finally, R-paclobutrazol had a significant effect on the microbial networks. The findings of the present study suggest that the use of S-paclobutrazol may accomplish both plant growth regulation and the minimization of effects of paclobutrazol on soil microbial communities.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148942DOI Listing
November 2021

Variety-Selective Rhizospheric Activation, Uptake, and Subcellular Distribution of Perfluorooctanesulfonate (PFOS) in Lettuce ( L.).

Environ Sci Technol 2021 07 25;55(13):8730-8741. Epub 2021 Jun 25.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, Hawaii 96822, United States.

Perfluorooctanesulfonate (PFOS) as an accumulative emerging persistent organic pollutant in crops poses severe threats to human health. Lettuce varieties that accumulate a lower amount of PFOS (low-accumulating crop variety, LACV) have been identified, but the regarding mechanisms remain unsolved. Here, rhizospheric activation, uptake, translocation, and compartmentalization of PFOS in LACV were investigated in comparison with those of high-accumulating crop variety (HACV) in terms of rhizospheric forms, transporters, and subcellular distributions of PFOS. The enhanced PFOS desorption from the rhizosphere soils by dissolved organic matter from root exudates was observed with weaker effect in LACV than in HACV. PFOS root uptake was controlled by a transporter-mediated passive process in which low activities of aquaporins and rapid-type anion channels were corrected with low expression levels of ( and ) and ( and ) genes in LACV roots. Higher PFOS proportions in root cell walls and trophoplasts caused lower root-to-shoot transport in LACV. The ability to cope with PFOS toxicity to shoot cells was poorer in LACV relative to HACV since PFOS proportions were higher in chloroplasts but lower in vacuoles. Our findings provide novel insights into PFOS accumulation in lettuce and further understanding of multiprocess mechanisms of LACV.
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http://dx.doi.org/10.1021/acs.est.1c01175DOI Listing
July 2021

Enantioselective metabolism of phenylpyrazole insecticides by rat liver microsomal CYP3A1, CYP2E1 and CYP2D2.

Pestic Biochem Physiol 2021 Jul 11;176:104861. Epub 2021 May 11.

College of Resources and Environment, Anhui Agricultural University, Key Laboratory of Agri-food Safety of Anhui Province, Hefei 230036, China. Electronic address:

The stereoselective difference of chiral pesticide enantiomers is an important factor of risk evaluation and the subject has received wide attention. In the present work, enantioselective metabolism of chiral phenylpyrazole insecticides including fipronil, ethiprole and flufiprole in rat liver microsomes was investigated in vitro. The result showed remarkable enantioselectivity for fipronil and ethiprole with the EF values of 0.11-0.58. The metabolite fipronil-sulfone was formed with the degradation of fipronil. R-Ethiprole to S-ethiprole transformation was observed, but not S-ethiprole to R-ethiprole. No enantioselective metabolism was observed for flufiprole with the EF values of 0.49-0.51. The enzymatic assays showed that the inhibition ratio of R-fipronil and S-ethiprole was 1.5-2.1times that of the corresponding enantiomers on CYP2E1 and CYP2D2 activity, leading to the enantioselective metabolism. The result of the homology modeling and molecular docking further revealed that S-fipronil (-7.56 kcal mol) and R-ethiprole (-6.45 kcal mol) performed better binding with CYP2E1 and CYP2D2, respectively. The results provided useful data for the risk evaluation of chiral phenylpyrazole insecticides on ecological safety and human health.
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http://dx.doi.org/10.1016/j.pestbp.2021.104861DOI Listing
July 2021

Enantioselective degradation of the organophosphorus insecticide isocarbophos in Cupriavidus nantongensis X1: Characteristics, enantioselective regulation, degradation pathways, and toxicity assessment.

J Hazard Mater 2021 09 7;417:126024. Epub 2021 May 7.

Anhui Provincial Key Laboratory for Quality and Safety of Agri-Products, School of Resource & Environment, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address:

The chiral pesticide enantiomers often show selective efficacy and non-target toxicity. In this study, the enantioselective degradation characteristics of the chiral organophosphorus insecticide isocarbophos (ICP) by Cupriavidus nantongensis X1 were investigated systematically. Strain X1 preferentially degraded the ICP R isomer (R-ICP) over the S isomer (S-ICP). The degradation rate constant of R-ICP was 42-fold greater than S-ICP, while the former is less bioactive against pest insects but more toxic to humans than the latter. The concentration ratio of S-ICP to R-ICP determines whether S-ICP can be degraded by strain X1. S-ICP started to degrade only when the ratio (C/C) was greater than 62. Divalent metal cations could improve the degradation ability of strain X1. The detected metabolites that were identified suggested a novel hydrolysis pathway, while the hydrolytic metabolites were less toxic to fish and green algae than those from P-O bond breakage. The crude enzyme degraded both R-ICP and S-ICP in a similar rate, indicating that enantioselective degradation was due to the transportation of strain X1. The strain X1 also enantioselectively degraded the chiral organophosphorus insecticides isofenphos-methyl and profenofos. The enantioselective degradation characteristics of strain X1 make it suitable for remediation of chiral organophosphorus insecticide contaminated soil and water.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126024DOI Listing
September 2021

Dihydromyricetin Imbues Antiadipogenic Effects on 3T3-L1 Cells via Direct Interactions with 78-kDa Glucose-Regulated Protein.

J Nutr 2021 Jul;151(7):1717-1725

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, USA.

Background: Obesity is among the most serious public health problems worldwide, with few safe pharmaceutical interventions. Natural products have become an important source of potential anti-obesity therapeutics. Dihydromyricetin (DHM) exerts antidiabetic effects. The biochemical target of DHM, however, has been unknown. It is crucial to identify the biochemical target of DHM for elucidating its physiological function and therapeutic value.

Objectives: The objective of this study was to identify the biochemical target of DHM.

Methods: An abundant antiadipogenic flavanonol was extracted from the herbal plant Ampelopsis grossedentata through bioassay-guided fractionation and characterized with high-resolution LC-MS and 1H and 13C nuclear magnetic resonance. Antiadipogenic experiments were done with mouse 3T3-L1 preadipocytes. A biochemical target of the chemical of interest was identified with drug affinity responsive target stability assay. Direct interactions between the chemical of interest and the protein target in vitro were predicted with molecular docking and subsequently confirmed with surface plasmon resonance. Expression levels of peroxisome proliferator-activated receptor γ (PPARγ), which is associated with 78-kDa glucose-regulated protein (GRP78), were measured with real-time qPCR.

Results: DHM was isolated, purified, and structurally characterized. Cellular studies showed that DHM notably reduced intracellular oil droplet formation in 3T3-L1 cells with a median effective concentration of 294 μM (i.e., 94 μg/mL). DHM targeted the ATP binding site of GRP78, which is associated with adipogenesis. An equilibrium dissociation constant between DHM and GRP78 was 21.8 μM. In 3T3-L1 cells upon treatment with DHM at 50 μM (i.e., 16 μg/mL), the expression level of PPARγ was downregulated to 53.9% of the solvent vehicle control's level.

Conclusions: DHM targets GRP78 in vitro. DHM is able to reduce lipid droplet formation in 3T3-L1 cells through a mode of action that is plausibly associated with direct interactions between GRP78 and DHM, which is a step forward in determining potential applications of DHM as an anti-obesity agent.
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http://dx.doi.org/10.1093/jn/nxab057DOI Listing
July 2021

Degradation of benzo[a]pyrene by halophilic bacterial strain Staphylococcus haemoliticus strain 10SBZ1A.

PLoS One 2021 25;16(2):e0247723. Epub 2021 Feb 25.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, Hawaii, United States of America.

The exploitation of petroleum oil generates a considerable amount of "produced water or petroleum waste effluent (PWE)" that is contaminated with polycyclic aromatic hydrocarbons (PAHs), including Benzo[a]pyrene (BaP). PWE is characterised by its high salinity, which can be as high as 30% NaCl, thus the exploitation of biodegradation to remove PAHs necessitates the use of active halophilic microbes. The strain 10SBZ1A was isolated from oil contaminated soils, by enrichment experiment in medium containing 10% NaCl (w/v). Homology analyses of 16S rRNA sequences identified 10SBZ1A as a Staphylococcus haemoliticus species, based on 99.99% homology (NCBI, accession number GI: MN388897). The strain could grow in the presence of 4-200 μmol l-1 of BaP as the sole source of carbon, with a doubling time of 17-42 h. This strain optimum conditions for growth were 37 oC, 10% NaCl (w/v) and pH 7, and under these conditions, it degraded BaP at a rate of 0.8 μmol l-1 per day. The strain 10SBZ1A actively degraded PAHs of lower molecular weights than that of BaP, including pyrene, phenanthrene, anthracene. This strain was also capable of removing 80% of BaP in the context of soil spiked with BaP (10 μmol l-1 in 100 g of soil) within 30 days. Finally, a metabolic pathway of BaP was proposed, based on the identified metabolites using liquid chromatography-high resolution tandem mass spectrometry. To the best of our knowledge, this is the first report of a halophilic BaP degrading bacterial strain at salinity > 5% NaCl.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247723PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939701PMC
August 2021

Development of a nanobody-based ELISA for the detection of the insecticides cyantraniliprole and chlorantraniliprole in soil and the vegetable bok choy.

Anal Bioanal Chem 2021 Apr 13;413(9):2503-2511. Epub 2021 Feb 13.

Beijing Key Laboratory of Biodiversity and Organic Farming, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China.

Cyantraniliprole and chlorantraniliprole are anthranilic diamide insecticides acting on ryanodine receptors. In this study, two camel-derived nanobodies (Nbs, named C1 and C2) recognizing cyantraniliprole as well as chlorantraniliprole were generated. C1-based enzyme-linked immunosorbent assays (ELISAs) for the detection of the two insecticides were developed. The half-maximum signal inhibition concentrations (IC) of cyantraniliprole and chlorantraniliprole by ELISA were 1.2 and 1.5 ng mL, respectively. This assay was employed to detect these two insecticides in soil and vegetables. The average recoveries of cyantraniliprole from both bok choy (Brassica chinensis L.) and soil samples were 90-129%, while those of chlorantraniliprole were in a range of 89-120%. The insecticide residues in soil and bok choy, which were collected from plots sprayed with cyantraniliprole and chlorantraniliprole, were simultaneously detected by the resulting ELISA and a high-performance liquid chromatography (HPLC) method, showing a satisfactory correlation. Higher concentrations of chlorantraniliprole than cyantraniliprole were detected in soil and vegetables, which indicates the longer persistence of chlorantraniliprole in the environment.
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http://dx.doi.org/10.1007/s00216-021-03205-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422987PMC
April 2021

Mdfi Promotes C2C12 Cell Differentiation and Positively Modulates Fast-to-Slow-Twitch Muscle Fiber Transformation.

Front Cell Dev Biol 2021 22;9:605875. Epub 2021 Jan 22.

National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, China.

Muscle development requires myoblast differentiation and muscle fiber formation. Myod family inhibitor (Mdfi) inhibits myogenic regulatory factors in NIH3T3 cells, but how Mdfi regulates myoblast myogenic development is still unclear. In the present study, we constructed an Mdfi-overexpression (Mdfi-OE) C2C12 cell line by the CRISPR/Cas9 system and performed RNA-seq on Mdfi-OE and wild-type (WT) C2C12 cells. The RNA-seq results showed that the calcium signaling pathway was the most significant. We also established the regulatory networks of Mdfi-OE on C2C12 cell differentiation and muscle fiber type transformation and identified hub genes. Further, both RNA-seq and experimental verification demonstrated that Mdfi promoted C2C12 cell differentiation by upregulating the expression of Myod, Myog, and Myosin. We also found that the positive regulation of Mdfi on fast-to-slow-twitch muscle fiber transformation is mediated by , , and its downstream genes, such as , , , , , , , and . In conclusion, our results demonstrated that Mdfi promotes C2C12 cell differentiation and positively modulates fast-to-slow-twitch muscle fiber transformation. These findings further our understanding of the regulatory mechanisms of Mdfi in myogenic development and muscle fiber type transformation. Our results suggest potential therapeutic targets for muscle- and metabolic-related diseases.
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http://dx.doi.org/10.3389/fcell.2021.605875DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862576PMC
January 2021

Genome, metabolic pathways and characteristics of cometabolism of dibenzothiophene and the biodiesel byproduct glycerol in Paraburkholderia sp. C3.

Bioresour Technol 2021 Apr 12;326:124699. Epub 2021 Jan 12.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.

Utilization of glycerol, a biodiesel byproduct, has not been well explored. In the present study, glycerol and the other carbon sources were studied for cometabolism of dibenzothiophene (DBT), a model chemical commonly used in bioremediation studies, by Paraburkholderia sp. C3. This study showed a direct association between rhamnolipids (RLs) biosynthesis and DBT biodegradation induced by different carbon sources in a Paraburkholderia specie. Glycerol can induce the strain C3 produce at least four RLs. The RL precursor is mainly derived from the fatty acid synthesis (FAS II) and β-oxidation pathway. The genome contained two (fabF and fabG) and four (fadA, fadE, fadB and echA) genes involved in FAS II and β-oxidation, respectively. The genome also carried the rhlA and rhlB genes involved in rhamnosyltransferase for RL biosynthesis and two DBT dioxygenase genes (nahAc and catA). The findings suggest a viable approach of using the biodiesel byproduct glycerol to remediate contaminated environments.
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http://dx.doi.org/10.1016/j.biortech.2021.124699DOI Listing
April 2021

Fusion expression of nanobodies specific for the insecticide fipronil on magnetosomes in Magnetospirillum gryphiswaldense MSR-1.

J Nanobiotechnology 2021 Jan 19;19(1):27. Epub 2021 Jan 19.

Beijing Key Laboratory of Biodiversity and Organic Farming, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China.

Background: Magnetic nanoparticles such as magnetosomes modified with antibodies allow a high probability of their interaction with targets of interest. Magnetosomes biomineralized by magnetotactic bacteria are in homogeneous nanoscale size and have crystallographic structure, and high thermal and colloidal stability. Camelidae derived nanobodies (Nbs) are small in size, thermal stable, highly water soluble, easy to produce, and fusible with magnetosomes. We aimed to functionalize Nb-magnetosomes for the analysis of the insecticide fipronil.

Results: Three recombinant magnetotactic bacteria (CF, CF+ , and CFFF) biomineralizing magnetosomes with different abundance of Nbs displayed on the surface were constructed. Compared to magnetosomes from the wild type Magnetospirillum gryphiswaldense MSR-1, all of the Nb-magnetosomes biosynthesized by strains CF, CF+ , and CFFF showed a detectable level of binding capability to fipronil-horseradish peroxidase (H2-HRP), but none of them recognized free fipronil. The Nb-magnetosomes from CFFF were oxidized with HO or a glutathione mixture consisting of reduced glutathione and oxidized glutathione in vitro and their binding affinity to H2-HRP was decreased, whereas that to free fipronil was enhanced. The magnetosomes treated with the glutathione mixture were employed to develop an enzyme-linked immunosorbent assay for the detection of fipronil in water samples, with average recoveries in a range of 78-101%.

Conclusions: The economical and environmental-friendly Nb-magnetosomes biomineralized by the bacterial strain MSR-1 can be potentially applied to nanobody-based immunoassays for the detection of fipronil or nanobody-based assays in general.
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http://dx.doi.org/10.1186/s12951-021-00773-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816308PMC
January 2021

Where are the new herbicides?

Pest Manag Sci 2021 Jun 2;77(6):2620-2625. Epub 2021 Feb 2.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan, China.

Herbicide resistance has become one of the foremost problems in crop production worldwide. New herbicides are required to manage weeds that have evolved resistance to the existing herbicides. However, relatively few herbicides with new modes of action (MOAs) have been discovered in the past two decades. Therefore, the discovery of new herbicides (i.e., new chemical classes or MOAs) remains a primary but ongoing strategy to overcome herbicide resistance and ensure crop production. In this mini-review, starting with the inherent characteristics of the target proteins and the inhibitor structures, we propose two strategies for the rational design of new herbicides and one computational method for the risk evaluation of target mutation-conferred herbicide resistance. The information presented here may improve the utilization of known targets and inspire the discovery of herbicides with new targets. We believe that these strategies may trigger the sustainable development of herbicides in the future. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.6285DOI Listing
June 2021

The first complete mitochondrial genome sequence of the korean endemic catfish (Actinopteri, Siluriformes, Siluridae).

Mitochondrial DNA B Resour 2019 Dec 13;5(1):131-132. Epub 2019 Dec 13.

School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea.

The is known as Korean endemic slender catfish. Despite its value as a biological resource, there is no complete mitochondrial genome sequence. The complete mitochondrial genome consisted of 16,524 bp including 22 transfer RNA (tRNAs), 2 ribosomal RNA (rRNAs), 13 protein-coding genes (PCGs), and A + T rich region. The overall base composition of was A + T: 56.1%, C + G: 43.9%, apparently with slight AT bias. Phylogenetic relationship showed that was closely related to
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http://dx.doi.org/10.1080/23802359.2019.1698336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721013PMC
December 2019

Comparison of three palm tree peroxidases expressed by Escherichia coli: Uniqueness of African oil palm peroxidase.

Protein Expr Purif 2021 03 7;179:105806. Epub 2020 Dec 7.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, 96822, USA. Electronic address:

Palm tree peroxidase has greater catalytic activity, stability and broad application prospects in comparison with horseradish peroxidase. However, slow growth, ecological destruction and high costs prohibit isolation of native peroxidases directly from palm trees. Bioreactor production of palm tree peroxidases would therefore be preferred to overcome such production limitations. Comparison of different recombinant glycan-free palm tree peroxidases would allow understanding the criticality of total glycans to the functions and characteristics. In the present study, African oil palm tree peroxidase expressed by Escherichia coli showed similar stability and 30-100-fold greater activity than that of recombinant royal palm tree peroxidases, but both of their comprehensive indexes were superior to the commercial, native horseradish peroxidase. Recombinant Chamaerops excelsa peroxidase showed no activity possibly due to incorrect protein folding. The results confirmed that recombinant expression by E. coli is potentially an effective means to obtain a mass of palm peroxidases with high activity and stability.
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http://dx.doi.org/10.1016/j.pep.2020.105806DOI Listing
March 2021

Isoorientin Inhibits Inflammation in Macrophages and Endotoxemia Mice by Regulating Glycogen Synthase Kinase 3.

Mediators Inflamm 2020 27;2020:8704146. Epub 2020 Oct 27.

Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, China.

Isoorientin has anti-inflammatory effects; however, the mechanism remains unclear. We previously found isoorientin is an inhibitor of glycogen synthase kinase 3 (GSK3) . Overactivation of GSK3 is associated with inflammatory responses. GSK3 is inactivated by phosphorylation at Ser9 (i.e., p-GSK3). Lithium chloride (LiCl) inhibits GSK3 and also increases p-GSK3 (Ser9). The present study investigated the anti-inflammatory effect and mechanism of isoorientin via GSK3 regulation in lipopolysaccharide- (LPS-) induced RAW264.7 murine macrophage-like cells and endotoxemia mice. LiCl was used as a control. While AKT phosphorylates GSK3, MK-2206, a selective AKT inhibitor, was used to activate GSK3 via AKT inhibition (i.e., not phosphorylate GSK3 at Ser9). The proinflammatory cytokines TNF-, IL-6, and IL-1 were detected by ELISA or quantitative real-time PCR, while COX-2 by Western blotting. The p-GSK3 and GSK3 downstream signal molecules, including NF-B, ERK, Nrf2, and HO-1, as well as the tight junction proteins ZO-1 and occludin were measured by Western blotting. The results showed that isoorientin decreased the production of TNF-, IL-6, and IL-1 and increased the expression of p-GSK3 and , similar to LiCl. Coadministration of isoorientin and LiCl showed antagonistic effects. Isoorientin decreased the expression of COX-2, inhibited the activation of ERK and NF-B, and increased the activation of Nrf2/HO-1 in LPS-induced RAW264.7 cells. Isoorientin increased the expressions of occludin and ZO-1 in the brain of endotoxemia mice. In summary, isoorientin can inhibit GSK3 by increasing p-GSK3 and regulate the downstream signal molecules to inhibit inflammation and protect the integrity of the blood-brain barrier and the homeostasis in the brain.
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http://dx.doi.org/10.1155/2020/8704146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641714PMC
September 2021

Chemical Nematicides: Recent Research Progress and Outlook.

J Agric Food Chem 2020 Nov 20;68(44):12175-12188. Epub 2020 Oct 20.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China.

Plant-parasitic nematodes have caused huge economic losses to agriculture worldwide and seriously threaten the sustainable development of modern agriculture. Chemical nematicides are still the most effective means to manage nematodes. However, the long-term use of organophosphorus and carbamate nematicides has led to a lack of field control efficacy and increased nematode resistance. To meet the huge market demand and slow the growth of resistance, new nematicides are needed to enter the market. The rational design and synthesis of new chemical scaffolds to screen for new nematicides is still a difficult task. We reviewed the latest research progress of nematicidal compounds in the past decade, discussed the structure-activity relationship and mechanism of action, and recommended some nematicidal active fragments. It is hoped that this review can update the recent progress on nematicide discoveries and provide new ideas for the design and mechanism of action studies of nematicides.
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http://dx.doi.org/10.1021/acs.jafc.0c02871DOI Listing
November 2020

Isoorientin, a GSK-3β inhibitor, rescues synaptic dysfunction, spatial memory deficits and attenuates pathological progression in APP/PS1 model mice.

Behav Brain Res 2021 02 16;398:112968. Epub 2020 Oct 16.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East-West Road, Honolulu, HI 96822, United States. Electronic address:

β-Amyloid (Aβ) elevation, tau hyperphosphorylation, and neuroinflammation are major hallmarks of Alzheimer's disease (AD). Glycogen synthase kinase-3β (GSK-3β) is a key protein kinase implicated in the pathogenesis of AD. Blockade of GSK-3β is an attractive therapeutic strategy for AD. Isoorientin, a 6-C-glycosylflavone, was previously shown to be a highly selective inhibitor of GSK-3β, while exerting neuroprotective effects in neuronal models of AD. In the present study, we evaluated the in vivo effects of isoorientin on GSK-3β, tau phosphorylation, Aβ deposition, neuroinflammatory response, long-term potentiation, and spatial memory in amyloid precursor protein/presenilin 1 (APP/PS1) transgenic mice using biochemical, electrophysiological, and behavioral tests. Chronic oral administration of isoorientin to APP/PS1 mice at 8 months of age attenuated multiple AD pathogenic hallmarks in the brains, including GSK-3β overactivation, tau hyperphosphorylation, Aβ deposition, and neuroinflammation. For neuroinflammation, isoorientin treatment reduced the number of activated microglia associated with Aβ-positive plaques, and in parallel reduced the levels of pro-inflammatory factors in the brains of APP/PS1 mice. Strikingly, isoorientin reversed deficits in synaptic long-term potentiation and spatial memory relevant to cognitive functions. Together, the findings suggest that isoorientin is a brain neuroprotector and may be a promising drug lead for treatment of AD and related neurodegenerative disorders.
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http://dx.doi.org/10.1016/j.bbr.2020.112968DOI Listing
February 2021

Enhanced biodegradation of organophosphorus insecticides in industrial wastewater via immobilized Cupriavidus nantongensis X1.

Sci Total Environ 2021 Feb 25;755(Pt 1):142505. Epub 2020 Sep 25.

Key Laboratory for Agri-Food Safety, School of Resource & Environment, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address:

Chlorpyrifos is an important organophosphorus insecticide. It is highly toxic to mammals and can pollute the environment. Cupriavidus nantongensis X1 can efficiently degrade chlorpyrifos. Immobilization technology can also improve the viability, stability and catalytic ability of bacteria. In this study, strain X1 was, therefore, captured on various composite immobilized carriers, sodium alginate (SA), diatomite (KLG), chitosan (CTS) and polyvinyl alcohol (PVA). The four types of immobilized beads (SA, SA + KLG, SA + CTS and SA + PVA) could form a slice and honeycomb structure to capture strain X1. The results showed that SA + CTS (SC) was an optimal material combination for the immobilization of strain X1 to degrade chlorpyrifos. Compared with SA-X1, after adding CTS, the specific surface area and adsorption capacity for chlorpyrifos were increased 3.4 and 1.7 fold, respectively. SC-X1 could degrade 96.6% of chlorpyrifos at 20 mg/L within 24 h and the degradation rate constant was 4.8 fold greater than immobilized strain LLBD2, a well-studied chlorpyrifos-degrading strain. The immobilized beads SC-X1 also showed a more stable and greater degradation ability than X1 free cells for chlorpyrifos in industrial wastewater. The synergy of adsorption and degradation of immobilized strain X1 is suitable for in-situ remediation of chlorpyrifos contaminated environment.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142505DOI Listing
February 2021

Oxalic Acid in Root Exudates Enhances Accumulation of Perfluorooctanoic Acid in Lettuce.

Environ Sci Technol 2020 10 8;54(20):13046-13055. Epub 2020 Oct 8.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, Hawaii 96822, United States.

Perfluorooctanoic acid (PFOA) is bioaccumulative in crops. PFOA bioaccumulation potential varies largely among crop varieties. Root exudates are found to be associated with such variations. Concentrations of low-molecular-weight organic acids (LMWOAs) in root exudates from a PFOA-high-accumulation lettuce variety are observed significantly higher than those from PFOA-low-accumulation lettuce variety ( < 0.05). Root exudates and their LMWOAs components exert great influences on the linear sorption-desorption isotherms of PFOA in soils, thus activating PFOA and enhancing its bioavailability. Among root exudate components, oxalic acid is identified to play a key role in activating PFOA uptake, with >80% attribution. Oxalic acid at rhizospheric concentrations (0.02-0.5 mM) can effectively inhibit PFOA sorption to soils by decreasing hydrophobic force, electrostatic attraction, ligand exchange, and cation-bridge effect. Oxalic acid enhances dissolution of metallic ions, iron/aluminum oxides, and organic matters from soils and forms oxalate-metal complexes, based on nuclear magnetic resonance spectra, ultraviolet spectra, and analyses of metal ions, iron/aluminum organometallic complexes, and dissolved organic carbon. The findings not only reveal the activation process of PFOA in soils by root exudates, particularly oxalic acid at rhizospheric concentrations, but also give an insight into the mechanism of enhancing PFOA accumulation in lettuce varieties.
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http://dx.doi.org/10.1021/acs.est.0c04124DOI Listing
October 2020

Effects of dibutyl phthalate contamination on physiology, phytohormone homeostasis, rhizospheric and endophytic bacterial communities of Brassica rapa var. chinensis.

Environ Res 2020 10 19;189:109953. Epub 2020 Jul 19.

Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, 50 Zhongling Street, Nanjing, 210014, China. Electronic address:

Phthalates are plasticizers and are ubiquitously detected in the environment, frequently at mg/kg levels. The present study aimed to evaluate the effects of dibutyl phthalate (DBP) on germination, growth, enzyme activity, phytohormone homeostasis and bacterial communities of two cultivars of Brassica rapa var. chinensis. The germination rate was decreased up to 20% compared to the control, and the growth of the vegetables was severely inhibited at the early stage when exposed to DBP at 20 mg/kg. Antioxidant defense enzyme activities and malondialdehyde (MDA) content increased upon exposure to DBP. A dose-response of auxin (IAA) was observed after a 2 d exposure. Gibberellin (GA3) and abscisic acid (ABA) responded at day 10 under DBP stress. GA3 did not show a clear dose-response effect and ABA increased about 3 times as the DBP concentration increased from 2 to 20 mg/L. Microbial population shifts were observed, especially in rhizosphere soil and roots. No obvious change occurred for the α diversity of rhizospheric bacteria among different treatments. Chao1, Shannon and Simpson indices of the root endophytic bacteria showed a decreasing trend with increasing DBP supplementation, while all the indices increased in shoot endophytic bacteria in comparison to the control. The results indicated that exposure to DBP may compromise the fitness of the leafy vagetables and alter the endophytic and rhizospheric bacteria, which might further affect the nutrients of the vegetables and alter ecosystem functions.
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http://dx.doi.org/10.1016/j.envres.2020.109953DOI Listing
October 2020

Characteristics of bacterial populations in an industrial scale petrochemical wastewater treatment plant: Composition, function and their association with environmental factors.

Environ Res 2020 10 18;189:109939. Epub 2020 Jul 18.

State Key Laboratory of Heavy Oil Processing, State Key Laboratory of Petroleum Pollution Control, China University of Petroleum-Beijing, Beijing, 102249, China.

The efficiency of petrochemical wastewater biological treatment is dependent upon complex bacterial communities. A well understanding of the structure and function of bacterial community and their association with environmental variables is essential for the elucidation of contaminant removal mechanisms and optimization of wastewater treatment processes. In this study, the bacterial communities and metabolic functions in the primary hydrolysis acidification unit (PHAU), cyclic activated sludge system (CASS), secondary hydrolysis acidification unit (SHAU), and biological aerated filter (BAF) of a petrochemical wastewater treatment plant (WWTP) were studied via Illumina high-throughput sequencing. The correlations between bacterial community and environmental variables were also investigated. The phylum Proteobacteria, Planctomycetes, Chloroflexi, Acidobacteria and Bacteroidetes were dominant in the petroleum WWTP. The bacterial communities varied with wastewater characteristics and operational parameters, as a result of the differences in biosystems functions. Phylogenetic analysis showed that the genes involved in the degradation of benzoate, nitrotoluene and aminobenzoate degradation were abundant in PHAU, and the genes related to the degradation of benzoate, aminobenzoate, chloroalkane, chloroalkene, caprolactam, naphthalene and toluene were abundant in CASS, SHAU and BAF. The Redundancy analysis (RDA) suggested that biochemical oxygen demand (BOD), NH-N and total nitrogen concentrations exhibited significant impacts in shaping the structure of bacterial community. Variance partitioning analysis (VPA) showed that 18.6% of the community variance was related to wastewater characteristics, higher than operational parameters of 4.5%. These results provide insight into microbial community structure and metabolic function during petrochemical wastewater treatment, and discern the relationships between bacterial community and environmental variables, which can provide basic data and a theoretical analysis of the design and operation optimization in petrochemical WWTP.
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http://dx.doi.org/10.1016/j.envres.2020.109939DOI Listing
October 2020

Antibiotics and Food Safety in Aquaculture.

J Agric Food Chem 2020 Oct 4;68(43):11908-11919. Epub 2020 Oct 4.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, Hawaii 96822, United States.

Antibiotics are widely used in aquaculture. Intensive farming drives indiscriminate use of antibiotics, which results in residues of antibiotics in cultured aquatic products and bacterial resistance. This perspective attempts to present a brief update on usage, regulations, residues, and potential human health risk of antibiotics used in aquaculture. Through the comprehensive literature review, we provide a view that the safety of aquatic products still requires further attention and more rigorous risk assessment. Finally, we make a few suggestions for future research directions: reduce the use of antibiotics to bring down the speed of resistance development and monitor resistant pathogens and genes, strictly manage the environmental sanitation of aquaculture and pay attention to the quality of water bodies introduced into aquaculture, seek international cooperation to establish an information bank of antibiotic residues and antibiotic-resistant genes, and set up a quantitative model to assess the risk of antibiotic resistance associated with the antibiotic residues.
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http://dx.doi.org/10.1021/acs.jafc.0c03996DOI Listing
October 2020

Recent Research Progress in and Perspectives of Mesoionic Insecticides: Nicotinic Acetylcholine Receptor Inhibitors.

J Agric Food Chem 2020 Oct 22;68(40):11039-11053. Epub 2020 Sep 22.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, Guizhou 550025, People's Republic of China.

Triflumezopyrim exemplifies a new class of mesoionic insecticides and has attracted increasing attention as a result of its unique structure, high level of insecticidal activity, new mechanisms of action, low toxicity toward non-target organisms, and environmental friendliness. It inhibits the nicotinic acetylcholine receptor and has high potency against sucking pests, including the brown planthopper (), which has developed serious resistance to conventional neonicotinoids and low cross-resistance to some newly developed neonicotinoids. This review focuses on the discovery, synthesis, structure-activity relationships, and mechanism of action of mesoionic insecticides. Finally, potential directions for the development of mesoionic insecticides are discussed.
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http://dx.doi.org/10.1021/acs.jafc.0c02376DOI Listing
October 2020

Bioaccumulation and Phytotoxicity and Human Health Risk from Microcystin-LR under Various Treatments: A Pot Study.

Toxins (Basel) 2020 08 14;12(8). Epub 2020 Aug 14.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.

Microcystin-LR (MC-LR) is prevalent in water and can be translocated into soil-crop ecosystem via irrigation, overflow (pollution accident), and cyanobacterial manure applications, threatening agricultural production and human health. However, the effects of various input pathways on the bioaccumulation and toxicity of MCs in terrestrial plants have been hardly reported so far. In the present study, pot experiments were performed to compare the bioaccumulation, toxicity, and health risk of MC-LR as well as its degradation in soils among various treatments with the same total amount of added MC-LR (150 μg/kg). The treatments included irrigation with polluted water (IPW), cultivation with polluted soil (CPS), and application of cyanobacterial manure (ACM). Three common leaf-vegetables in southern China were used in the pot experiments, including L., L., and L. All leaf vegetables could bioaccumulate MC-LR under the three treatments, with much higher MC-LR bioaccumulation, especially root bioconcentration observed in ACM treatment than IPW and CPS treatments. An opposite trend in MC-LR degradation in soils of these treatments indicated that ACM could limit MC-LR degradation in soils and thus promote its bioaccumulation in the vegetables. MC-LR bioaccumulation could cause toxicity to the vegetables, with the highest toxic effects observed in ACM treatment. Similarly, bioaccumulation of MC-LR in the edible parts of the leaf-vegetables posed 1.1~4.8 fold higher human health risks in ACM treatment than in IPW and CPS treatments. The findings of this study highlighted a great concern on applications of cyanobacterial manure.
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http://dx.doi.org/10.3390/toxins12080523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472386PMC
August 2020

Rice root exudates enhance desorption and bioavailability of phthalic acid esters (PAEs) in soil associating with cultivar variation in PAE accumulation.

Environ Res 2020 07 28;186:109611. Epub 2020 Apr 28.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, 96822, USA.

Phthalic acid esters (PAEs) is a class of prevalent pollutants in agricultural soil, threating food safety through crop uptake and accumulation of PAEs. Accumulation of PAEs varies largely among crop species and cultivars. Nevertheless, how root exudates affect PAE bioavailability, dissipation, uptake and accumulation is still not well understood. In the present study, desorption and pot experiments were designed to investigate how root exudates from high-(Peizataifeng) and low-(Fengyousimiao) PAE accumulating rice cultivars affect soil PAE bioavailability, dissipation, and accumulation variation. Rice root exudates including low molecular weight organic acids (LMWOAs) of Peizataifeng and Fengyousimiao could enhance desorption of two typical PAE compounds, di-n-butyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP), from aged soil to their available fractions by increasing soil dissolved organic carbon (DOC), thus improving their bioavailability in soil. Peizataifeng produced twice higher amounts of oxalic acid, critic acid and malonic acid in root exudates, and exhibited stronger effects on enhancing desorption and bioavailability of DBP and DEHP than Fengyousimiao. Higher (by about 50%) total organic carbon contents of root exudates from Peizataifeng led to higher (by 10-30%) soil microbial biomass carbon and nitrogen than Fengyousimiao, and thus promoted more PAE dissipation from soil than Fengyousimiao. Nevertheless, higher (by 20-50%) soil DOC and significantly higher PAE bioavailability in the soils planted Peizataifeng resulted in greater (by 53-93%) PAE accumulation in roots and shoots of Peizataifeng than Fengyousimiao, confirming by higher (by 1.82-3.48 folds) shoot and root bioconcentration factors of Peizataifeng than Fengyousimiao. This study reveals that the difference in root exudate extent and LMWOAs between Peizataifeng and Fengyousimiao differentiates PAE accumulation.
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http://dx.doi.org/10.1016/j.envres.2020.109611DOI Listing
July 2020

Aerobic sludge granulation in shale gas flowback water treatment: Assessment of the bacterial community dynamics and modeling of bioreactor performance using artificial neural network.

Bioresour Technol 2020 Oct 15;313:123687. Epub 2020 Jun 15.

State Key Laboratory of Heavy Oil Processing, State Key Laboratory of Petroleum Pollution Control, China University of Petroleum-Beijing, Beijing 102249, China. Electronic address:

Flowback water from shale gas extraction is highly saline and comprises complex organic substances, thereby posing a significant challenge for the environmental management of the unconventional natural gas industry. In this work, an aerobic granular sludge (AGS) method was successfully used for the treatment of flowback water from shale gas extraction. The formed AGS had a diameter of 0.25-2.0 mm and the total sludge volume index was 23.40 mL g. The AGS efficiently removed COD, NH-N and TN by 70.1%, 92.1%, and 59.2%, respectively. The bacterial communities responsible for the removal of nitrogen and degradation of organics were enriched in AGS. The dynamics of contaminant removal was further explained with a three-layered artificial neural network model. The results showed that the initial concentration of COD, TDS, NH-N and TN governed the contaminants' removal. As for operating parameters, aerating time showed a strong effect on NH-N and TN removal, whereas settling time impacted the COD removal.
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http://dx.doi.org/10.1016/j.biortech.2020.123687DOI Listing
October 2020

Mini-review: recent advances in the identification and application of sex pheromones of gall midges (Diptera: Cecidomyiidae).

Pest Manag Sci 2020 Dec 17;76(12):3905-3910. Epub 2020 Jul 17.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, USA.

Most gall midges (Diptera: Cecidomyiidae) are cryptic agricultural pests. These tiny insects are prone to lurk in cargoes and spread over long distances. Their larvae feed in plant tissues, so their infestations are imperceptible until crop damage and economic loss appear. The application of sex pheromones is an efficient method with which to monitor and manage gall midges. The present review focuses on recent advances in the accurate identification of gall midge sex pheromones based on rapidly evolving analytical techniques and their use in field trials in integrated pest management. To date, sex pheromones from 19 species of gall midges have been identified and reported, and sex pheromone-based monitoring systems have been developed and commercialized for at least ten gall midge species. All monitoring systems were tested in various experiments with a modicum of success. In addition to further studies of sex pheromones, identification of plant-derived volatiles may offer potentials for the manipulation of behavioral response of gall midges, which can be used in dispensers for surveillance and control purposes. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.5949DOI Listing
December 2020
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