Publications by authors named "Qing Sun"

555 Publications

Peptidomic Analysis of Neonate Umbilical Cord Blood for the Identification of Endogenous Peptides Involved in Hypoxic-Ischemic Encephalopathy.

Front Pediatr 2021 25;9:718704. Epub 2021 Aug 25.

Department of Pediatric, Jingjiang People's Hospital Affiliated to Yangzhou University, Jingjiang, China.

Neonatal hypoxic-ischemic encephalopathy (HIE) is a common neurological disorder triggered by perinatal cerebral ischemia and hypoxia. Accumulating evidence has shown that peptides have neuroprotective effects in nerve injury. However, the function of endogenous peptides in the pathogenesis of HIE has not been studied. In the present study, a comparative peptidomic profile was performed in the serum of the human umbilical cord blood with HIE (three patients) and the control group (three health control) by liquid chromatography-mass spectrometry (LC-MS). Our study demonstrated that a total of 49 peptides derived from 25 precursor proteins were differentially expressed in the serum of HIE compared with normal controls, including 33 upregulated peptides and 16 downregulated peptides. Each of the differentially expressed peptides has specific characteristics, including pI, Mw, and cleavage pattern. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that the precursor proteins of differentially expressed peptides participate in the different biological process. Moreover, among the 49 differentially expressed peptides, 21 peptides were identified from the fibrinogen chain family, which plays a role in neurological diseases, suggesting that these peptides may play an important role in maintaining brain health. In conclusion, our results showed a comparative peptidomic profile from human umbilical cord blood of HIE patients and normal controls. These dysregulated peptides may have potentially important functions in umbilical cord blood with HIE and may be involved in the pathogenesis of the HIE.
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http://dx.doi.org/10.3389/fped.2021.718704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424085PMC
August 2021

Role of hyperbaric oxygen in glioma: a narrative review.

Med Gas Res 2022 Jan-Mar;12(1):1-5

Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.

Gliomas are common brain mass with a high mortality rate. Patients with gliomas have a severely bad outcome, with an average survive duration less 15 months because of high recurrent rate and being resistant to radio-therapy and chemistry drugs therapy. Hyperbaric oxygen is extensively taken as an adjuvant treatment for various disease conditions. To know the characteristics of hyperbaric oxygen as a remedy for gliomas, we find that, in general, hyperbaric oxygen shows an obviously positive effect on the treatment of gliomas, and it can also relieve the complications caused by postoperative radiotherapy and chemotherapy of gliomas. Whereas, several researches have shown that hyperbaric oxygen promotes glioma progression.
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http://dx.doi.org/10.4103/2045-9912.324589DOI Listing
September 2021

Synergistical Performance Modification of Epoxy Resin by Nanofillers and Carboxyl-Terminated Liquid Nitrile-Butadiene Rubber.

Materials (Basel) 2021 Aug 16;14(16). Epub 2021 Aug 16.

School of Electrical Engineering, Xi'an University of Technology, Xi'an 710048, China.

Epoxy composite materials are widely used in power equipment. As the voltage level increases, the requirement of material properties, including electrical, thermal, and mechanical, has also increased. Introducing thermally conductive nanofiller to the epoxy/liquid rubber composites system is an effective approach to improve heat performance, but the effects of thermally conductive nanofillers on relaxation characteristics remain unclarified. In this paper, nano-alumina (nano-AlO) and nano-boron nitride (nano-BN) have been employed to modify the epoxy/carboxyl-terminated liquid nitrile-butadiene rubber (epoxy/CTBN) composites system. The thermal conductivity and glass transition temperature of different formula systems have been measured. The effect of the nanofillers on the relaxation behaviors of the resin matrix has been investigated. Results show that the different kinds of nanofillers will introduce different relaxation processes into the matrix and increase the conductivity at the same time. This study can provide a theoretical basis for the synergistic improvement of multiple properties of epoxy resin composites.
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http://dx.doi.org/10.3390/ma14164601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402213PMC
August 2021

Carbon Nitride Supported High-Loading Fe Single-Atom Catalyst for Activation of Peroxymonosulfate to Generate O with 100 % Selectivity.

Angew Chem Int Ed Engl 2021 Sep 31;60(40):21751-21755. Epub 2021 Aug 31.

Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle, Nanchang Hangkong University, Nanchang, Jiangxi, 330063, P. R. China.

Singlet oxygen ( O ) is an excellent active species for the selective degradation of organic pollutions. However, it is difficult to achieve high efficiency and selectivity for the generation of O . In this work, we develop a graphitic carbon nitride supported Fe single-atoms catalyst (Fe /CN) containing highly uniform Fe-N active sites with a high Fe loading of 11.2 wt %. The Fe /CN achieves generation of 100 % O by activating peroxymonosulfate (PMS), which shows an ultrahigh p-chlorophenol degradation efficiency. Density functional theory calculations results demonstrate that in contrast to Co and Ni single-atom sites, the Fe-N sites in Fe /CN adsorb the terminal O of PMS, which can facilitate the oxidization of PMS to form SO , and thereafter efficiently generate O with 100 % selectivity. In addition, the Fe /CN exhibits strong resistance to inorganic ions, natural organic matter, and pH value during the degradation of organic pollutants in the presence of PMS. This work develops a novel catalyst for the 100 % selective production of O for highly selective and efficient degradation of pollutants.
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http://dx.doi.org/10.1002/anie.202109488DOI Listing
September 2021

ER-α36 Promotes the Malignant Progression of Cervical Cancer Mediated by Estrogen HMGA2.

Front Oncol 2021 14;11:712849. Epub 2021 Jul 14.

Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, China.

Objectives: Estrogen is proven to promote the malignant behaviors of many cancers its receptors. Estrogen receptor alfa 36 (ER-α36) is a newly identified isoform of estrogen receptor alfa (ER-α), the role of ER-α36 in regulating the effects of estrogen and its potential impact on human cervical cancer is poorly understood.

Methods: Immunohistochemistry staining was used to evaluate the expression of ER-α36, estrogen receptor alfa 66 (ER-α66) and their prognostic values in cervical cancer. The effects of ER-α36 and ER-α66 on the proliferation and metastasis of cervical cancer were measured . A xenograft tumor assay was used to study the tumorigenesis role of ER-α36 . Furthermore, the functional gene at the downstream of ER-α36 was obtained next-generation sequencing, and the biological functions of high mobility group A2 (HMGA2) in cervical cancer cells were investigated .

Results: ER-α36 was over-expressed in cervical cancer tissues and elevated ER-α36 expression was associated with poor prognosis in cervical cancer patients. High expression of ER-α36 promoted the proliferation, invasion and metastasis of cervical cancer cells mediated by estrogen, while silencing ER-α36 had the opposite effects. Further research showed that HMGA2 was a downstream target of ER-α36 in cervical cancer cells. The oncogenic effect of ER-α36 was attenuated after HMGA2 knockdown.

Conclusions: High expression of ER-α36 was correlated with a poor prognosis in cervical cancer by regulating HMGA2. ER-α36 could be a prognostic biomarker and a target for cervical cancer treatment.
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http://dx.doi.org/10.3389/fonc.2021.712849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317436PMC
July 2021

Partial Gyrus Rectus Resection as a Technique to Improve the Exposure to the Anterior Communicating Artery Complex through the Junctional Triangle: A Quantitative Study.

J Neurol Surg B Skull Base 2021 Jul 5;82(Suppl 3):e211-e216. Epub 2020 May 5.

Department of Neurosurgery, The Loyal and Edith Davis Neurosurgical Research Laboratory, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, United States.

 The junctional triangle, formed by the distal A1 anterior cerebral artery (ACA) segment, the proximal A2 ACA segment, and the medial surface of gyrus rectus (GR), is a corridor of access to superiorly and posteriorly projecting anterior communicating artery (AComA) aneurysms that is widened by GR retraction or resection. Exposure of the AComA complex through the junctional triangle after GR resection has not been previously quantitatively evaluated.  GR resection extent and increase in artery exposure through the junctional triangle were assessed in this study.  This study was conducted in the laboratory with a pterional approach, exposing the AComA complex.  Ten sides of five cadaveric heads were considered.  Exposure extent of ipsilateral and contralateral A1, A2, and AComA and accessibility of branches coming off the AComA complex were measured before and after GR resection. The GR was resected until sufficient bilateral A2 and contralateral A1 exposures were achieved. GR resection span was measured.  The mean (standard deviation) resected span of GR was 7 ± 3.9 mm. After GR resection, the exposed span of the ipsilateral A2 increased from 2 ± 0.7 mm to 4 ± 1.1 mm (  = 0.001); contralateral A2 exposure increased from 3 ± 1.5 mm to 4 ± 1.1 mm (  = 0.03). Contralateral recurrent artery of Heubner (RAH) and orbitofrontal artery were accessible in five and eight specimens, respectively, before GR resection and in all 10 after resection.  GR resection improves exposure of bilateral A2 segments through the junctional triangle. Exposure improvement is greater for the ipsilateral A2 than contralateral A2. The junctional triangle concept is enhanced by partial GR resection during surgery for superior and posterior AComA aneurysms.
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http://dx.doi.org/10.1055/s-0040-1710517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289494PMC
July 2021

Granulocyte-Macrophage Colony Stimulating Factor in Single Blastocyst Conditioned Medium as a Biomarker for Predicting Implantation Outcome of Embryo.

Front Immunol 2021 30;12:679839. Epub 2021 Jun 30.

Shenzhen Key Laboratory of Reproductive Immunology for Peri-Implantation, Shenzhen Zhongshan Institute for Reproduction and Genetics, Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen, China.

Background: It is highly desirable to develop new strategies based on secretomics to more accurately selection of embryos with the highest developmental potential for transfer. Granulocyte-macrophage colony-stimulating factor (GM-CSF) has been reported to promote embryo development and pregnancy establishment. However, the predictive value of GM-CSF in single blastocyst selection remains unclear. This study is to determine the concentration of GM-CSF in human single-blastocyst conditioned medium (SBCM) and to evaluate its association with embryo quality and pregnancy outcome.

Methods: The patients with ≤38 years of age receiving the first cycle of assisted reproductive therapy were included in this study. The patients who had <4 top-quality embryos formed by the fertilized two pronuclear zygotes on day 3 were excluded. A total of 126 SBCM samples (SBCMs) were included, of which blastocysts from 77 SBCMs were later transferred in subsequent frozen-thawed embryo transfer. The concentrations of GM-CSF were detected by single-molecule array (SIMOA) and analyzed for their possible association with embryo quality and pregnancy outcomes. The top-quality embryo (TQ), positive HCG (HP), clinical pregnancy (CP), and ongoing pregnancy (OP) rates were determined and compared between groups divided based on GM-CSF concentrations.

Results: The detection rate of GM-CSF was found to be 50% in all SBCMs. There were significant differences in TQ rate, HP rate, CP rate and OP rate among high concentration group, medium concentration group and low concentration group. Both GM-CSF alone or GM-CSF combined with the morphological score (MS) had a greater AUC of ROC curve than that of MS alone to predict the pregnancy outcome, and GM-CSF combined with MS had the highest AUC.

Conclusions: The concentration of GM-CSF in SBCM was detected at fg/ml levels, which was associated with embryo quality and pregnancy outcome. Collectively, GM-CSF may be used as a biomarker for prediction of pregnancy outcome and selection of embryos with high developmental potential for transfer in assisted reproductive technology (ART).
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http://dx.doi.org/10.3389/fimmu.2021.679839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278135PMC
June 2021

A multidimensional rational design of nickel-iron sulfide and carbon nanotubes on diatomite via synergistic modulation strategy for supercapacitors.

J Colloid Interface Sci 2021 Dec 27;603:799-809. Epub 2021 Jun 27.

School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address:

Based on their characteristics, transition metal layered double hydroxides have been of great scientific interest for their use in supercapacitors. Up until now, severe aggregation and low intrinsic conductivity have been the major hurdles for their application. In this work, nickel-iron sulfide nanosheets (NiFeSx) and carbon nanotubes (CNTs) were synthesized on diatomite using chemical vapor deposition and a two-step hydrothermal method to overcome these challenges. Synthesis of this composite successfully exploits the synergistic effect of multicomponent materials to improve the electrochemical performance. Diatomite is selected as a substrate to provide preferable surroundings for the uniform dispersion of nanomaterial on its surface, which enlarges the active sites that come in contact with the electrolytes, significantly improving the electrochemical properties. Combined with high conductivity and a synchronous sulfurization effect, the [email protected]@[email protected] electrode delivered a high specific capacitance of 552F g at a current density of 1 A g, a good rate capability of 68.4% retention at 10 A g, and superior cycling stability of 89.8% capacitance retention after 5000 cycles at 5 A g. Furthermore, an asymmetric supercapacitor assembled via [email protected]@[email protected] and graphene delivered a maximum energy density of 28.9 Wh kg and a maximum power density of 9375 W kg at a potential of 1.5 V. This research lays the groundwork for ideal material preparation as well as a rational design for the electrode material, including property enhancement of diatomite-based material for use in supercapacitors.
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http://dx.doi.org/10.1016/j.jcis.2021.06.131DOI Listing
December 2021

Microplastic-water partitioning of two states halogenated PAHs: Solute and sol.

Environ Res 2021 Jul 6;202:111634. Epub 2021 Jul 6.

School of Urban Planning and Design, Peking University Shenzhen Graduate School, Shenzhen, 518055, China. Electronic address:

The complex interactions of contaminants with microplastics significantly affect ecological risk assessments. Studies of the sorption behavior of freely dissolved hydrophobic organic contaminants (HOCs) on microplastics are common. However, concentrations of HOCs in the actual aquatic environment sometimes exceed their water solubility. A possible explanation is that a microplastic-sol-water three-phase medium (TPM) is formed in the actual water environment. Both states HOCs (in solute and sol) have the potential to migrate to particles suspended in water. To confirm this view, four kinds of microplastics and eight halogenated polycyclic aromatic hydrocarbons (HPAHs) were selected to examine the partitioning of HPAHs between microplastics and water (sol and solute). Both monolayer and multilayer coverage of HPAHs onto microplastics occurred, and chemical sorption dominated the pseudo-sorption mechanism. The microplastic-water partition ratios of HPAHs (0.12-0.74) were approximately four to five orders of magnitude lower than their corresponding K values, suggesting that mechanisms other than sorption were involved. Apparently, the sol HPAHs contributed almost identically large increments to both microplastics and water, and closed the gap. For microplastic-supported HPAHs, the contribution of the sol fraction was more than triple that of the dissolved fraction; the key influencing factor was the water solubility of HPAHs.
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http://dx.doi.org/10.1016/j.envres.2021.111634DOI Listing
July 2021

Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic LM187.

J Microbiol Biotechnol 2021 Aug;31(8):1144-1153

Key Laboratory of Food Biotechnology, College of Food and Bioengineering, Xihua University, Chengdu 610039, Sichuan, P.R. China.

A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as subsp. mesenteroides. The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 10 Da. Analysis of the monosaccharide composition revealed that the molecule was mainly composed of glucose. The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds. H and C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid. Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures. In addition, rEPS414 exhibited strong in vitro antioxidant activity. These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.
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http://dx.doi.org/10.4014/jmb.2103.03055DOI Listing
August 2021

Synthesis and Surface Behaviour of NDI Chromophores Mounted on a Tripodal Scaffold: Towards Self-Decoupled Chromophores for Single-Molecule Electroluminescence.

Chemistry 2021 Aug 22;27(47):12144-12155. Epub 2021 Jul 22.

Institute of Nanotechnology, Karlsruhe Institute of Technology, P.O. Box 3640, 76021, Karlsruhe, Germany.

This paper reports the efficient synthesis, absorption and emission spectra, and the electrochemical properties of a series of 2,6-disubstituted naphthalene-1,4,5,8-tetracarboxdiimide (NDI) tripodal molecules with thioacetate anchors for their surface investigations. Our studies showed that, in particular, the pyrrolidinyl group with its strong electron-donating properties enhanced the fluorescence of such core-substituted NDI chromophores and caused a significant bathochromic shift in the absorption spectrum with a correspondingly narrowed bandgap of 1.94 eV. Cyclic voltammetry showed the redox properties of NDIs to be influenced by core substituents. The strong electron-donating character of pyrrolidine substituents results in rather high HOMO and LUMO levels of -5.31 and -3.37 eV when compared with the parental unsubstituted NDI. UHV-STM measurements of a sub-monolayer of the rigid tripodal NDI chromophores spray deposited on Au(111) show that these molecules mainly tend to adsorb flat in a pairwise fashion on the surface and form unordered films. However, the STML experiments also revealed a few molecular clusters, which might consist of upright oriented molecules protruding from the molecular island and show electroluminescence photon spectra with high electroluminescence yields of up to 6×10 . These results demonstrate the promising potential of the NDI tripodal chromophores for the fabrication of molecular devices profiting from optical features of the molecular layer.
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http://dx.doi.org/10.1002/chem.202101264DOI Listing
August 2021

as potential source of natural bioactive compounds: Nutritional, phytochemical, and antioxidant properties.

Food Sci Nutr 2021 Jun 3;9(6):2968-2984. Epub 2021 May 3.

Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining China.

Royle ex Decne. (family , subfamily , genus ) is a wild berry fruit with illustrated health-promoting features, which widely distributed in Northwest China are deficiently exploited. This study aimed to assess the potential of a as a source of natural bioactive compounds through characterizing its nutraceutical characteristics, phytochemicals properties, and antioxidant ability. Fresh berries were quantitatively analyzed for proximate composition, minerals, vitamins, amino acids, total polyphenols, total flavonoids, anthocyanins, procyanidin, and polysaccharides contents through China National Food Safety Standard; the characterization and identification of extracts of wild berries obtained with ethanol 30%, ethanol 50%, and ethanol 95% were firstly performed by UPLC-Triple-TOF-MS. Furthermore, antioxidant activity of the ethanol extract was evaluated via different assay methods such as DPPH, ABTS, and FRAP. The results indicated that the most important bioactive composition was procyanidin (0.72%), polyphenols (0.49%), total flavonoids (0.38%), vitamin C (64.6 mg/100g FW), and K (218.44 mg/100 g FW), and a total of 95 compounds were detected with polyphenols, flavonoids, and proanthocyanidins as the dominant, and also ethanol extract possessed stronger antioxidant activity. These results suggested that fruit has great potential in protecting human health, with the focus on the development of functional products.
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http://dx.doi.org/10.1002/fsn3.2256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194758PMC
June 2021

Evaluating scenarios for carbon reduction using different tableware in China.

Sci Total Environ 2021 Oct 5;791:148279. Epub 2021 Jun 5.

School of Urban Planning and Design, Peking University Shenzhen Graduate School, Shenzhen 518055, China. Electronic address:

Global consumption of disposable plastic tableware (DPT) is massive because it is durable, light and inexpensive. Using the life cycle assessment method, we found that DPT for per person per meal emitted 597 g of CO and was far more than that of reusable plastic tableware (RPT, 7.00 g), ceramic tableware (9.55 g) and straw tableware (14.6 g). If the demand growth for DPT continues, 416 MT of CO will be emitted due to DPT consumption by 2050 globally. We further explored strategies to reduce CO emissions by examining the life cycles of four types of tableware according to sensitivity analysis. According to our results, if the recycling rate of DPT reaches 60% at the end-of-life stage, 50% of CO emissions can be cut; if dishwashing instead of hand washing is used to clean RPT, ceramic, and straw tableware, approximately 64%, 71%, and 23% of CO emissions can be reduced, respectively. If 60% of DPT is replaced by RPT, this plastic tableware will halve carbon emissions. If the rate reaches 100%, carbon emissions will be reduced by 92%. Although the CO emissions of the three types of tableware other than DPT are relatively small, RPT will bring other environmental burdens and human health risks, ceramic tableware is bulky and its additives are toxic. Straw tableware combines practical and safety performance. The results show that the choice of straw tableware plays a significant role in curbing the greenhouse effect without compromising consumer safety.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148279DOI Listing
October 2021

Primary colonic lymphoma: report of two cases and a literature review.

J Int Med Res 2021 Jun;49(6):3000605211017037

Department of Colorectal Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Primary colonic lymphoma is a very rare malignant tumor with no standard treatment. We report two cases of primary colonic lymphoma successfully treated with surgery and chemotherapy, and chemotherapy alone, respectively. The first case was a 61-year-old woman who presented with abdominal pain of more than 1 month. The patient was diagnosed with a colonic tumor, and immunohistochemical examinations confirmed the initial diagnosis of colonic lymphoma. The patient underwent laparoscopic-assisted right hemicolectomy followed by postoperative adjuvant chemotherapy with the cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) regimen, combined with targeted therapy with rituximab (R-CHOP). The second case was a 78-year-old man who presented with a complaint of abdominal distention for more than 1 year. Diffuse large B-cell lymphoma was definitively diagnosed by immunohistochemical examinations, and the patient underwent systemic chemotherapy with the R-CHOP regimen. Primary colonic lymphoma is a rare type of non-Hodgkin's lymphoma (NHL), and the clinical treatment is not standardized, unlike for many other types of lymphoma. Therefore, treatment is mainly based on the patient's symptoms to determine whether surgery or systemic chemotherapy is appropriate. Rituximab is effective in some patients and may play an important role in the treatment of unresectable or asymptomatic colonic lymphoma.
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http://dx.doi.org/10.1177/03000605211017037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182193PMC
June 2021

First Report of Causing Leaf Sheath and Leaf Spot on Maize in China.

Plant Dis 2021 Jun 2. Epub 2021 Jun 2.

Nantong University, 66479, College of Life Sciences, Nantong, China;

In November 2020, leaf sheath on maize () was detected in southeastern Jiangsu (Nantong municipality; 120.54° E, 31.58° N) in China. Physiologically mature plants, 13 weeks of cultivation (at the harvest stage), exhibited red-brown lesions in stem and leaves, and dried-up stem (Figure 1). The symptoms were observed on approximately 95% of the maize plants in a 0.8 ha maize field surrounded by old sorghum fields and the crop yield was decreased by 70-85% with respect previous years, when no disease symptoms were detected. Small pieces, approximately 0.3 cm in size, of symptomatic tissue were surface sterilized in 1.5% NaOCl for 1 min, and washed twice with sterile ddHO. The pathogen was isolated (one isolate was obtained) and cultured on PDA medium, containing chloramphenicol (50 µg/mL), under darkness at 26 ºC for 3 days. Amplification of internal transcribed spacer (), large subunit (), actin () and β-tubulin () genes was performed using , , and primers, respectively (Ma et al. 2021). Sequences were submitted to GenBank under accession numbers MW800180 (), MW800361 (), MW845677 () and MW892439 (). Blast search revealed that the sequence had 100% (492/492 bp) homology with LY-D-1-1, MT604999, had 98% (1075/1091 bp) homology with GZDS2018BXT010, MK516207, had 96% (214/222 bp) homology with M3, MK044832, and had 99% (498/499 bp) homology with BJ-F1, MF987525. Molecular phylogenetic tree was constructed using MEGA7 to confirm the identity of the pathogen. The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model, and the tree with the highest likelihood (-1774.9882) is shown in Figure 2. , and spp. found causing leaf spot on maize were included in the phylogenetic tree (Liu et al. 2021; Reyes Gaige et al. 2020; Chang et al. 2016). To confirm pathogenicity, a sterilized spatula was used to make wounds (3 mm diameter, 1 mm depth) on the stem and leaves of 2-week old maize plants. A solution containing 1 × 108 spores/mL (20 µL) was injected in the wound, whereas sterilized ddHO was used in the control experiment. Inoculated plants were maintained in a growth chamber at 28 °C and 60% relative humidity for 3 days, observing fast-growing necrotic lesions in both stem and leaves. The pathogen was recovered from the infected plants and its identity was confirmed by morphological and sequence analyses. Microscope observations indicated the presence of chlamydospores, oval conidia (3 × 5 µm) and round pycnidia (60-100 µm diameter), and agree with those previously reported for the morphology of (Bao et al. 2019). During last 2 years, was reported to cause leaf spot on a number of relevant agricultural crops in China, including taro, , tea, rice and wheat (Du et al. 2020; Li et al. 2020; Liu et al. 2020a, 2020b), confirming the expansion and host promiscuity of this pathogen. The pathogen was also reported to cause leaf spot on maize in Brazil in 2004 (Do Amaral et al. 2004); however, this is the first report of causing leaf sheath and leaf spot on maize in China. Maize an important agricultural crop in China with more than 168 million tons produced in 2019. The observed yield loss and disease incidence of the isolated strain suggest that may be a threat to maize production in China.
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http://dx.doi.org/10.1094/PDIS-04-21-0746-PDNDOI Listing
June 2021

Characterization of genotype-phenotype correlation with MORC2 mutated Axonal Charcot-Marie-Tooth disease in a cohort of Chinese patients.

Orphanet J Rare Dis 2021 05 31;16(1):244. Epub 2021 May 31.

Department of Neurology, Xuanwu Hospital, Capital Medical University, Chang Chun Street, Beijing, 100053, People's Republic of China.

Background: Charcot-Marie-Tooth (CMT) disease is an exciting field of study, with a growing number of causal genes and an expanding phenotypic spectrum. The microrchidia family CW-type zinc finger 2 gene (MORC2) was newly identified as a causative gene of CMT2Z in 2016. We aimed to describe the phenotypic-genetic spectrum of MORC2-related diseases in the Chinese population.

Methods: With the use of Sanger sequencing and Next Generation Sequencing (NGS) technologies, we screened a cohort of 284 unrelated Chinese CMT2 families. Pathogenicity assessments of MORC2 variants were interpreted according to the ACMG guidelines. Potential pathogenic variants were confirmed by Sanger sequencing.

Results: We identified 4 different heterozygous MORC2 mutations in four unrelated families, accounting for 1.4% (4/284). A novel mutation c.1397A>G p. D466G was detected in family 1 and all affected patients presented with later onset axonal CMT with hyperCKemia. The patient in family 2 showed a spinal muscular atrophy (SMA)-like disease with cerebellar hypoplasia and mental retardation, with a hot spot de novo mutation c.260C>T p. S87L. The twin sisters in family 3 were identified as having the most common mutation c.754C>T p. R252W and suffered from axonal motor neuropathy with high variability in disease severity and duration. The patient in family 4 developed an early onset axonal motor and sensory neuropathy, with a reported mutation c.1220G>A p.C407Y. All identified mutations associated with MORC2-related neuropathies are localized in the N-terminal ATPase module.

Conclusions: Our study confirmed that MORC2-related neuropathies exist in the Chinese population at a relatively high mutation rate. We revealed a complex genotype-phenotype correlation with MORC2 mutations. This report adds a new piece to the puzzle of the genetics of CMT and contributes to a better understanding of the disease mechanisms.
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http://dx.doi.org/10.1186/s13023-021-01881-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166055PMC
May 2021

Exotic Vortex States with Discrete Rotational Symmetry in Atomic Fermi Gases with Spin-Orbital-Angular-Momentum Coupling.

Phys Rev Lett 2021 May;126(19):193401

School of Science, Westlake University, 18 Shilongshan Road, Hangzhou 310024, Zhejiang Province, China.

We investigate the superfluidity of a two-component Fermi gas with spin-orbital-angular-momentum coupling (SOAMC). Because of the intricate interplay of SOAMC, two-photon detuning and atom-atom interaction, a family of vortex ground states emerges in a broad parameter regime of the phase diagram, in contrast to the usual case where an external rotation or magnetic field is generally required. More strikingly, an unprecedented vortex state, which breaks the continuous rotational symmetry to a discrete one spontaneously, is predicted to occur. The underlying physics are elucidated and verified by numerical simulations. The unique density distributions of the predicted vortex states enable a direct observation in experiment.
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http://dx.doi.org/10.1103/PhysRevLett.126.193401DOI Listing
May 2021

Correction to: Nrf2 is required to maintain the self-renewal of glioma stem cells.

BMC Cancer 2021 May 21;21(1):582. Epub 2021 May 21.

Department of Neurosurgery in Jinling Hospital, Neurosurgical Institution of People's Liberation Army of China, No. 305, East Zhongshan Road, Nanjing, 210002, Jiangsu, China.

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http://dx.doi.org/10.1186/s12885-021-08338-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139154PMC
May 2021

Programming gene expression in multicellular organisms for physiology modulation through engineered bacteria.

Nat Commun 2021 05 11;12(1):2689. Epub 2021 May 11.

Department of Chemical Engineering, Texas A&M University, College Station, TX, USA.

A central goal of synthetic biology is to predictably and efficiently reprogram living systems to perform computations and carry out specific biological tasks. Although there have been many advances in the bio-computational design of living systems, these advances have mainly been applied to microorganisms or cell lines; programming animal physiology remains challenging for synthetic biology because of the system complexity. Here, we present a bacteria-animal symbiont system in which engineered bacteria recognize external signals and modulate animal gene expression, twitching phenotype, and fat metabolism through RNA interference toward gfp, sbp-1, and unc-22 gene in C. elegans. By using genetic circuits in bacteria to control these RNA expressions, we are able to program the physiology of the model animal Caenorhabditis elegans with logic gates. We anticipate that engineered bacteria can be used more extensively to program animal physiology for agricultural, therapeutic, and basic science applications.
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http://dx.doi.org/10.1038/s41467-021-22894-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113242PMC
May 2021

Toll-like receptor 3 ablation prevented high-fat diet-induced obesity and metabolic disorder.

J Nutr Biochem 2021 09 6;95:108761. Epub 2021 Jun 6.

School of Public Health and Joint China-US Research Center for Environment and Pulmonary Diseases, Zhejiang Chinese Medical University, Hangzhou, China. Electronic address:

Inflammation in insulin-sensitive tissues (e.g., liver, visceral adipose tissue [VAT]) plays a major role in obesity and insulin resistance. Recruitment of innate immune cells drives the dysregulation of glucose and lipid metabolism. We aimed to seek the role of Toll like receptor 3 (TLR3), a pattern recognition receptor involved in innate immunity, obesity and the metabolic disorder. TLR3 expression in liver and VAT from diet induced obese mice and in VAT from overweight women was examined. Body weight, glucose homeostasis and insulin sensitivity were evaluated in TLR3 wild-type and knockout (KO) mice on a chow diet (CD) or high-fat diet for 15 weeks. At euthanasia, blood was collected, and plasma biochemical parameters and adipokines were determined with commercial kits. Flow cytometry was used to measure macrophage infiltration and activation in VAT. Standard western blot, immunohistochemistry and quantative PCR were used to assess molecules in pathways about lipid and glucose metabolism, insulin and inflammation in tissues of liver and VAT. Utilizing human and animal samples, we found that expression of TLR3 was upregulated in the liver and VAT in obese mice as well as VAT in overweight women. TLR3-deficiency protected against high-fat diet induced obesity, glucose intolerance, insulin resistance and lipid accumulation. Lipolysis was enhanced in VAT and hepatic lipogenesis was inhibited in TLR3 KO animals. Macrophages infiltration into adipose tissue was attenuated in TLR3 KO mice, accompanied with inhibition of NF-κB-dependent AMPK/Akt signaling pathway. These findings demonstrated that TLR3 ablation prevented obesity and metabolic disorders, thereby providing new mechanistic links between inflammation and obesity and associated metabolic abnormalities in lipid/glucose metabolism.
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http://dx.doi.org/10.1016/j.jnutbio.2021.108761DOI Listing
September 2021

Genus Ribes Linn. (Grossulariaceae): A comprehensive review of traditional uses, phytochemistry, pharmacology and clinical applications.

J Ethnopharmacol 2021 Aug 30;276:114166. Epub 2021 Apr 30.

Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai, 810008, China; Key Laboratory of Restoration Ecology of Cold Area in Qinghai Province, Xining, Qinghai, 810008, China.

Ethnopharmacological Relevance: The genus Ribes Linn., which belongs to the Grossulariaceae family, contains 160 species distributed mainly in temperate and cold regions of the Northern Hemisphere. There are 59 species in southwest, northwest and northeast China. Some species of Ribes have been used as traditional and local medicines for the treatment of glaucoma, cardiovascular disease, stomachache, hepatitis, hyperlipidemia, hypertension and other ailments. However, the data provided in recent years have not been collated and compared.

Aim Of The Study: This review aims to summarize the current status of ethnopharmacological uses, phytochemistry, pharmacology, clinical applications, and pharmacokinetics of the genus Ribes to better understand the therapeutic potential of the genus Ribes in the future and hope to provide a relatively novel perspective for further clinical application on the genus.

Materials And Methods: The literature on Ribes was collected through a series of scientific search engines including Elsevier, ACS, Springer, Web of Science, PubMed, Google Scholar, Baidu Scholar, Wiley, China National Knowledge Infrastructure (CNKI) and books.

Results: Ribes species have been used for detoxification, glaucoma, cardiovascular disease, stomachache, hepatitis, hyperlipidemia, hypertension and other ailments. These plants mainly contain phenolic glycosides, flavonoids, proanthocyanidins, polysaccharides, etc. Most traditional uses are related to biological activity and have been confirmed by modern research. Pharmacological studies in vitro and in vivo revealed that the extracts and pure compounds possessed significant hypolipidemic, antioxidant, anti-inflammatory, antitumor, antibacterial, and antiviral activity, eyesight protection and other effects.

Conclusions: The traditional uses, phytochemistry, pharmacology, pharmacokinetics, and clinical applications described in this article explained that the Ribes species has numerous activities, and these findings will promote further action in the area of mechanism research. However, very few preclinical and clinical studies have focused on the toxicology and pharmacokinetics of crude extracts and pure compounds from the genus Ribes. Moreover, several clinical evidence to support the health benefits of Ribes plants. The development of new medicines based on Ribes species as ingredients may be restricted. The pharmacological activity, clinical efficacy and safety of Ribes species need to be verified by systematic and comprehensive preclinical studies and clinical trials.
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http://dx.doi.org/10.1016/j.jep.2021.114166DOI Listing
August 2021

Assessment for the Correlation Between Diabetic Retinopathy and Metabolic Syndrome: A Cross-Sectional Study.

Diabetes Metab Syndr Obes 2021 22;14:1773-1781. Epub 2021 Apr 22.

Department of Endocrinology, Fuling Center Hospital of Chongqing City, Chongqing, 408000, People's Republic of China.

Purpose: In this study, we explored the correlation between diabetic retinopathy (DR) and metabolic syndrome (MetS) among diabetes mellitus (DM) patients.

Methods: Logistic regression analysis was utilized to test the effects of MetS and its indicators on the incidence of DR and vision-related functional burden. The spline smoothing functions of continuous indicators of MetS were used to establish the logistic generalized additive model (GAM). The effective degree of freedom (EDF) =1 was served as a sign of linear relationship. EDF>1 was a sign of a more complex association between MetS and DR.

Results: The proportion of difficulties of looking for objects on the crowded shelves in the DR group was higher than that in the non-DR group (19.40 vs 12.10, <0.05). Elevated fasting glucose (Glu) and blood pressure levels were related to the vision-related functional burden. The risk of DR development increased by 6% [95% confidence interval (CI): 1.03-1.09, <0.001] and 1% (95% CI: 1.01-1.02, =0.004) per 1 unit increase in Glu and systolic blood pressure (SBP) of DM patients, respectively. In the univariate GAM, Glu had a linear effect on DR (EDF=1, <0.001) with a positive correlation after controlling SBP. And there was a nonlinear correlation between SBP and DR after controlling Glu (EDF=2.44, =0.024).

Conclusion: Both Glu and blood pressure were associated with the occurrence of DR and vision-related functional burden. Controlling the levels of Glu and blood pressure may reduce the risk of DR and vision loss among DM patients.
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http://dx.doi.org/10.2147/DMSO.S265214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075313PMC
April 2021

Occupational Health Risk Assessment of Benzene, Toluene, and Xylene in Shanghai.

Biomed Environ Sci 2021 Apr;34(4):290-298

Department of Occupation Health and Toxicology, School of Public Health, Fudan University, Shanghai 200000, China.

Objective: This study was designed to conduct a retrospective and systematic occupational health risk assessment (OHRA) of enterprises that used benzene, toluene, and xylene (BTX) in Shanghai, China.

Methods: All data for the study were obtained from 1,705 occupational health examination and evaluation reports from 2013 to 2017, and a semiquantitative model following Chinese OHRA guidelines (GBZ/T 298-2017) was applied for the assessment.

Results: The selected enterprises using BTX were mainly involved in manufacturing of products. Using the exposure level method, health risk levels associated with exposure to BTX were classified as medium, negligible, or low. However, the risk levels associated with benzene and toluene were significantly different according to job types, with gluers and inkers exhibiting greater health risks. For the same job type, the health risk levels assessed using the comprehensive index method were higher than those using the exposure level method.

Conclusion: Our OHRA reveals that workers who are exposed to BTX still face excessive health risk. Additionally, the risk level varied depending on job categories and exposure to specific chemicals. Therefore, additional control measures recommended by OHRA guidelines are essential to reduce worker exposure levels.
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http://dx.doi.org/10.3967/bes2021.038DOI Listing
April 2021

Polyphenol-Containing Nanoparticles: Synthesis, Properties, and Therapeutic Delivery.

Adv Mater 2021 Jun 19;33(22):e2007356. Epub 2021 Apr 19.

Yong Loo Lin School of Medicine and Faculty of Engineering, National University of Singapore, Singapore, 119077, Singapore.

Polyphenols, the phenolic hydroxyl group-containing organic molecules, are widely found in natural plants and have shown beneficial effects on human health. Recently, polyphenol-containing nanoparticles have attracted extensive research attention due to their antioxidation property, anticancer activity, and universal adherent affinity, and thus have shown great promise in the preparation, stabilization, and modification of multifunctional nanoassemblies for bioimaging, therapeutic delivery, and other biomedical applications. Additionally, the metal-polyphenol networks, formed by the coordination interactions between polyphenols and metal ions, have been used to prepare an important class of polyphenol-containing nanoparticles for surface modification, bioimaging, drug delivery, and disease treatments. By focusing on the interactions between polyphenols and different materials (e.g., metal ions, inorganic materials, polymers, proteins, and nucleic acids), a comprehensive review on the synthesis and properties of the polyphenol-containing nanoparticles is provided. Moreover, the remarkable versatility of polyphenol-containing nanoparticles in different biomedical applications, including biodetection, multimodal bioimaging, protein and gene delivery, bone repair, antibiosis, and cancer theranostics is also demonstrated. Finally, the challenges faced by future research regarding the polyphenol-containing nanoparticles are discussed.
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http://dx.doi.org/10.1002/adma.202007356DOI Listing
June 2021

Controllable morphology of electrospun nanofiber membranes with tunable groove structure and the enhanced filtration performance for ultrafine particulates.

Nanotechnology 2021 May 12;32(31). Epub 2021 May 12.

School of Materials Science & Engineering, Beijing Institute of Fashion Technology, Beijing 100029, People's Republic of China.

As researchers are striving to develop high-performance filtration membranes with hierarchical micro/nano structures, the challenges and costs of processing often limit creative innovation. Here, we propose a polyethersulfone/polyacrylonitrile (PES/PAN) nanofiber membrane with groove structure by electrospinning and facile post-processing. The resulted membrane can form a groove structure on the surface of the fiber after being soaked in chloroform, thereby increasing the collision probability and extending the residence time for ultrafine particulates and improving the filtration efficiency. The groove structure can be attributed to the solubility of PES constituent in chloroform, while PAN constituent will not be dissolved, thus forming a high-performance nanofiber membrane with high filtration efficiency (ca. 99.54%) and withstand pressure drop (ca. 133.9 Pa) for dioctyl phthalate aerosol particles with diameter of 0.3m. The results show that this convenient and low-cost fabrication technology can be used to prepare high-performance nanofiber membrane based air filters that have broad application prospects in respiratory protective equipment.
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http://dx.doi.org/10.1088/1361-6528/abf8daDOI Listing
May 2021

Sex-based differences in fecal short-chain fatty acid and gut microbiota in irritable bowel syndrome patients.

J Dig Dis 2021 May;22(5):246-255

Department of Gastroenterology, Peking University Third Hospital, Beijing, China.

Objective: To explore alterations in fecal short-chain fatty acids (SCFA) and gut microbiota in patients with diarrhea-predominant irritable bowel disease (IBS-D) and their relationships with clinical manifestations.

Methods: We recruited 162 patients with IBS-D and 66 healthy controls (HC). Their manifestations and psychological status were evaluated using the IBS severity scoring system and the Hospital Anxiety and Depression Scale (HADS). Colorectal visceral sensitivity was evaluated using a barostat. Systemic inflammation was evaluated using plasma cytokine levels. Fecal SCFA were quantified using ultra-performance liquid chromatography-tandem mass spectrometry, and fecal microbiota communities were analyzed using 16S rRNA sequencing.

Results: More men presented with IBS-D than women in our patient cohort. Patients with IBS-D had more severe manifestations, higher HADS score, and a higher rate of previous infectious enteritis than HC. Notably, female patients had significantly higher HADS scores than male patients. Male patients had significantly higher levels of plasma interleukin (IL)-12, fecal propionate and colorectal visceral sensitivity than male HC, while no differences were observed between female patients and female HC. Fecal acetate, butyrate and valerate correlated with the initial visceral sensory threshold, stressors, and IL-10 and IL-12 levels. The propionate-producing Prevotella 9 genus was significantly increased in male patients and positively correlated with fecal propionate.

Conclusion: Distinct sex-based differences in clinical manifestations, fecal SCFA and microbiota richness are found in Chinese patients with IBS-D, which may be used to diagnose dysbiosis in these patients.
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http://dx.doi.org/10.1111/1751-2980.12988DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8252430PMC
May 2021

A general approach to high-efficiency perovskite solar cells by any antisolvent.

Nat Commun 2021 Mar 25;12(1):1878. Epub 2021 Mar 25.

Kirchhoff Institute for Physics and Centre for Advanced Materials, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.

Deposition of perovskite films by antisolvent engineering is a highly common method employed in perovskite photovoltaics research. Herein, we report on a general method that allows for the fabrication of highly efficient perovskite solar cells by any antisolvent via manipulation of the antisolvent application rate. Through detailed structural, compositional, and microstructural characterization of perovskite layers fabricated by 14 different antisolvents, we identify two key factors that influence the quality of the perovskite layer: the solubility of the organic precursors in the antisolvent and its miscibility with the host solvent(s) of the perovskite precursor solution, which combine to produce rate-dependent behavior during the antisolvent application step. Leveraging this, we produce devices with power conversion efficiencies (PCEs) that exceed 21% using a wide range of antisolvents. Moreover, we demonstrate that employing the optimal antisolvent application procedure allows for highly efficient solar cells to be fabricated from a broad range of precursor stoichiometries.
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http://dx.doi.org/10.1038/s41467-021-22049-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994557PMC
March 2021

Head barotrauma.

Intern Emerg Med 2021 Mar 25. Epub 2021 Mar 25.

Dept. of Military and Special Medicine, No. 971 Hospital of the PLA Navy, No. 22 Minjiang Rd. Shinan District, Qingdao, 266071, China.

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http://dx.doi.org/10.1007/s11739-021-02709-9DOI Listing
March 2021

Cropping systems alter hydraulic traits of barley but not pea grown in mixture.

Plant Cell Environ 2021 Sep 27;44(9):2912-2924. Epub 2021 Apr 27.

Institute of Agricultural Sciences, ETH Zurich, Zurich, Switzerland.

Extreme events such as drought and heatwaves are among the biggest challenges to agricultural production and food security. However, the effects of cropping systems on drought resistance of arable crops via their hydraulic behaviour remain unclear. We investigated how hydraulic traits of a field-grown pea-barley (Pisum sativum L. and Hordeum vulgare L.) mixture were affected by different cropping systems, that is, organic and conventional farming with intensive or conservation tillage. Xylem vulnerability to cavitation of both species was estimated by measuring the pressure inducing 50% loss of hydraulic conductivity (P ), while the water stress plants experienced in the field were assessed using native percentage loss of hydraulic conductivity (nPLC). Pea and barley showed contrasting hydraulic behaviours: pea was less vulnerable to xylem cavitation and less stressed than barley; cropping systems affected the xylem vulnerability of barley, but not of pea. Barley grown under conventional farming with no tillage was more vulnerable and stressed than under organic farming with intensive tillage. nPLC proved to be a valuable indicator for plant water stress. Our results highlight the impact of cropping systems on crop xylem vulnerability and drought resistance, thus plant hydraulic traits, for protecting food security under future climate.
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http://dx.doi.org/10.1111/pce.14054DOI Listing
September 2021
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