Publications by authors named "Qing Shen"

257 Publications

Cardiac troponin T is elevated and increases longitudinally in ALS patients.

Amyotroph Lateral Scler Frontotemporal Degener 2021 Jun 21:1-8. Epub 2021 Jun 21.

Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.

To test whether high-sensitivity cardiac troponin T (hs-cTnT) could act as a diagnostic or prognostic biomarker in ALS, comparing hs-cTnT to neurofilament light (NfL). We performed a case-control study, including 150 ALS patients, 28 ALS mimics, and 108 healthy controls, and a follow-up study of the ALS patients, during 2014-2020 in Stockholm, Sweden. We compared concentrations of hs-cTnT in plasma and NfL in the cerebrospinal fluid between cases and controls. To evaluate the diagnostic performance, we calculated the area under the curve (AUC). Hazard ratios (HRs) were estimated from Cox models to assess associations between hs-cTnT and NfL at ALS diagnosis and risk of death. The longitudinal analysis measured changes of hs-cTnT and NfL since ALS diagnosis. We noted higher levels of hs-cTnT in ALS patients (median: 16.5 ng/L) than in ALS mimics (11 ng/L) and healthy controls (6 ng/L). Both hs-cTnT and NfL could distinguish ALS patients from ALS mimics, with higher AUC noted for NfL (AUC 0.88; 95%CI 0.79-0.97). Disease progression correlated weakly with hs-cTnT (Pearson's  = 0.18,  = 0.04) and moderately with NfL (Pearson's  = 0.41,  < 0.001). Shorter survival was associated with higher levels of NfL at diagnosis (HR 1.08, 95%CI 1.04-1.11), but not hs-cTnT. hs-cTnT increased (12.61 ng/L per year, 95%CI 7.14-18.06) whereas NfL decreased longitudinally since ALS diagnosis. NfL is a stronger diagnostic and prognostic biomarker than hs-cTnT for ALS. However, hs-cTnT might constitute a disease progression biomarker as it increases longitudinally. The underlying causes for this increase need to be investigated.
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http://dx.doi.org/10.1080/21678421.2021.1939384DOI Listing
June 2021

Surface-Modified Graphene Oxide/Lead Sulfide Hybrid Film-Forming Ink for High-Efficiency Bulk Nano-Heterojunction Colloidal Quantum Dot Solar Cells.

Nanomicro Lett 2020 May 16;12(1):111. Epub 2020 May 16.

Faculty of Informatics and Engineering, The University of Electro-Communications, Tokyo, 182-8585, Japan.

Solution-processed colloidal quantum dot solar cells (CQDSCs) is a promising candidate for new generation solar cells. To obtain stable and high performance lead sulfide (PbS)-based CQDSCs, high carrier mobility and low non-radiative recombination center density in the PbS CQDs active layer are required. In order to effectively improve the carrier mobility in PbS CQDs layer of CQDSCs, butylamine (BTA)-modified graphene oxide ([email protected]) is first utilized in PbS-PbX (X = I, Br) CQDs ink to deposit the active layer of CQDSCs through one-step spin-coating method. Such surface treatment of GO dramatically upholds the intrinsic superior hole transfer peculiarity of GO and attenuates the hydrophilicity of GO in order to allow for its good dispersibility in ink solvent. The introduction of [email protected] in CQDs layer can build up a bulk nano-heterojunction architecture, which provides a smooth charge carrier transport channel in turn improves the carrier mobility and conductivity, extends the carriers lifetime and reduces the trap density of PbS-PbX CQDs film. Finally, the [email protected]/PbS-PbX hybrid CQDs film-based relatively large-area (0.35 cm) CQDSCs shows a champion power conversion efficiency of 11.7% which is increased by 23.1% compared with the control device.
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http://dx.doi.org/10.1007/s40820-020-00448-8DOI Listing
May 2020

Impact of air-frying on the plasmalogens deterioration and oxidation in oyster revealed by mild acid hydrolysis and HILIC-MS-based lipidomics analysis.

Electrophoresis 2021 Jun 14. Epub 2021 Jun 14.

Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, P. R. China.

Oyster is rich in plasmalogens that are ether phospholipids with biological functions to human body. Air-frying is a popular technique for preparing delicious oyster but makes the plasmalogens vulnerable to oxidation. In this study, the effect of air-frying processing on plasmalogens oxidation was studied by lipidomic approach. Plasmalogens were always mixed with normal phospholipids, thus the lipid extract was treated with mild acid hydrolysis to rapidly degrade plasmalogens owing to the acid lability of vinyl ether linkage at sn-1 position. After hydrophilic interaction chromatography MS/MS analysis, there were three plasmalogen classes, plasmanylcholine, plasmanylethanolamine, and plasmanylinositol, completely separated, and each plasmalogen molecular species was identified and quantified. It indicated that the content of plasmalogens underwent an obvious decrease during the air-frying process. To weaken such effect, the influence of air-frying temperature was further inspected by multivariate statistical analyses. The main variables, including the ions of m/z 756.4927, 784.5486, 828.5812, etc., were revealed by unsupervised principle component analysis, supervised orthogonal partial least-square analysis, and variable importance in projection plot. As a conclusion, air-frying has health benefits in reducing fat content but destructive to plasmalogens, thus interventions are recommended to prevent the degradation of plasmalogens.
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http://dx.doi.org/10.1002/elps.202100106DOI Listing
June 2021

The role of sodium in stabilizing tin-lead (Sn-Pb) alloyed perovskite quantum dots.

J Mater Chem A Mater 2021 Mar 19;9(20):12087-12098. Epub 2021 Mar 19.

Materials Simulation and Modelling, Department of Applied Physics, Eindhoven University of Technology 5600 MB Eindhoven The Netherlands

Narrow-bandgap CsSn Pb I perovskite quantum dots (QDs) show great promise for optoelectronic applications owing to their reduced use of toxic Pb, improved phase stability, and tunable band gaps in the visible and near-infrared range. The use of small ions has been proven beneficial in enhancing the stability and photoluminescence quantum yield (PLQY) of perovskite QDs. The introduction of sodium (Na) has succeeded in boosting the PLQY of CsSnPbI QDs. Unfortunately, the initial PLQY of the Na-doped QDs undergoes a fast degradation after one-day storage in solution, hindering their practical applications. Using density functional theory (DFT) calculations and molecular dynamics (AIMD) simulations, we study the effect of Na ions on the strength of surface bonds, defect formation energies, and the interactions between surface ligands and perovskite QDs. Our results suggest that Na ions enhance the covalent bonding of surface tin-iodine bonds and form strong ionic bonding with the neighboring iodine anions, thus suppressing the formation of I and Sn vacancies. Furthermore, Na ions also enhance the binding strength of the surface ligands with the perovskite QD surface. However, according to our AIMD simulations, the enhanced surface ligand binding is only effective on a selected surface configuration. While the position of Na ions remains intact on a CsI-terminated surface, they diffuse vigorously on an MI-terminated surface. As a result, the positive effect of Na vanishes with time, explaining the relatively short lifetime of the experimentally obtained high PLQYs. Our results indicate that engineering the surface termination of the QDs could be the next step in maintaining the favorable effect of Na doping for a high and stable PLQY of Sn-Pb QDs.
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http://dx.doi.org/10.1039/d1ta00955aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148221PMC
March 2021

Multi-Arm PEG/Peptidomimetic Conjugate Inhibitors of DR6/APP Interaction Block Hematogenous Tumor Cell Extravasation.

Adv Sci (Weinh) 2021 06 18;8(11):e2003558. Epub 2021 Mar 18.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, School of Biomedical Engineering, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200032, China.

The binding of amyloid precursor protein (APP) expressed on tumor cells to death receptor 6 (DR6) could initiate the necroptosis pathway, which leads to necroptotic cell death of vascular endothelial cells (ECs) and results in tumor cells (TCs) extravasation and metastasis. This study reports the first inhibitor of DR6/APP interaction as a novel class of anti-hematogenous metastatic agent. By rationally utilizing three combined strategies including selection based on phage display library, d-retro-inverso modification, and multiple conjugation of screened peptidomimetic with 4-arm PEG, the polymer-peptidomimetic conjugate PEG-tAHP-DRI (tetra-(D-retro-inverso isomer of AHP-12) substitued 4-arm PEG ) is obtained as the most promising agent with the strongest binding potency (K  = 51.12 × 10  m) and excellent pharmacokinetic properties. Importantly, PEG-tAHP-DRI provides efficient protection against TC-induced ECs necroptosis both in vitro and in vivo. Moreover, this ligand exhibits prominent anti-hematogenous metastatic activity in serval different metastatic mouse models (B16F10, 4T1, CT26, and spontaneous lung metastasis of 4T1 orthotopic tumor model) and displays no apparent detrimental effects in preliminary safety evaluation. Collectively, this study demonstrates the feasibility of exploiting DR6/APP interaction to regulate hematogenous tumor cells transendothelial migration and provides PEG-tAHP-DRI as a novel and promising inhibitor of DR6/APP interaction for developments of anti-hematogenous metastatic therapies.
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http://dx.doi.org/10.1002/advs.202003558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188212PMC
June 2021

Passivating Quantum Dot Carrier Transport Layer with Metal Salts.

ACS Appl Mater Interfaces 2021 Jun 8. Epub 2021 Jun 8.

Cavendish Laboratory, University of Cambridge, Cambridge CB3 0HE, United Kingdom.

Quantum dots (QDs) have a wide range of applications in the field of optoelectronics. They have been playing multiple roles within the configuration of a device, by serving as the building blocks for both the active layer and the carrier transport layer. While the performance of various optoelectronic devices has been steadily improving via developments in passivating the QD active layer, the possible improvement via passivation of the QD-based carrier transport layer has been largely overlooked. Here, with lead sulfide QD photovoltaics as the platform of study, we demonstrate that the device performance can be significantly improved by passivating the QD hole transport layer (HTL) with zinc salt post-treatments. The power conversion efficiency is improved from 8.7% of the reference device to 10.2% and 9.5% for devices with zinc acetate (ZnAc)- and zinc iodide (ZnI)-treated HTLs, respectively. Transient absorption spectroscopy confirms that both treatments effectively reduce band-tail states and increase carrier lifetime of the HTLs. Further elemental analysis shows that ZnAc provides a higher amount of Zn for passivation while maintaining the function of HTL by allowing essential p-doping oxidation. In contrast, the additional I passivation from ZnI inhibits p-doping oxidation and limits the function of HTL. This work demonstrates the potential of improving device performance by passivating the QD-based HTLs, and the method developed is likely applicable to other optoelectronic devices.
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http://dx.doi.org/10.1021/acsami.1c06410DOI Listing
June 2021

Polyadenylation-related isoform switching in human evolution revealed by full-length transcript structure.

Brief Bioinform 2021 May 11. Epub 2021 May 11.

Laboratory of Bioinformatics and Genomic Medicine, Institute of Molecular Medicine, Peking University, Beijing, China.

Rhesus macaque is a unique nonhuman primate model for human evolutionary and translational study, but the error-prone gene models critically limit its applications. Here, we de novo defined full-length macaque gene models based on single molecule, long-read transcriptome sequencing in four macaque tissues (frontal cortex, cerebellum, heart and testis). Overall, 8 588 227 poly(A)-bearing complementary DNA reads with a mean length of 14 106 nt were generated to compile the backbone of macaque transcripts, with the fine-scale structures further refined by RNA sequencing and cap analysis gene expression sequencing data. In total, 51 605 macaque gene models were accurately defined, covering 89.7% of macaque or 75.7% of human orthologous genes. Based on the full-length gene models, we performed a human-macaque comparative analysis on polyadenylation (PA) regulation. Using macaque and mouse as outgroup species, we identified 79 distal PA events newly originated in humans and found that the strengthening of the distal PA sites, rather than the weakening of the proximal sites, predominantly contributes to the origination of these human-specific isoforms. Notably, these isoforms are selectively constrained in general and contribute to the temporospatially specific reduction of gene expression, through the tinkering of previously existed mechanisms of nuclear retention and microRNA (miRNA) regulation. Overall, the protocol and resource highlight the application of bioinformatics in integrating multilayer genomics data to provide an intact reference for model animal studies, and the isoform switching detected may constitute a hitherto underestimated regulatory layer in shaping the human-specific transcriptome and phenotypic changes.
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http://dx.doi.org/10.1093/bib/bbab157DOI Listing
May 2021

Factors Affecting Microbial Contamination on the Back Surface of Worn Soft Contact Lenses.

Optom Vis Sci 2021 05;98(5):512-517

Faculty of Medicine and Health, School of Optometry and Vision Science, University of New South Wales, Sydney, New South Wales, Australia.

Significance: The results of this study demonstrate that Smart Touch Technology packaging, which is designed to reduce and simplify contact lens handling before insertion, is effective in reducing the frequency of bacterial contamination of the back surface of contact lenses after short-term wear.

Purpose: The purpose of this study was to investigate the effect of lens packaging type, chelating agent, and finger contamination on microbial contamination on the back surface of worn soft contact lenses.

Methods: Twenty-five subjects completed each contralateral lens wear comparison in this randomized study: Smart Touch Technology versus conventional blister packaging for (1) silicone hydrogel lenses with ethylenediaminetetraacetic acid (EDTA) and (2) hydrogel lenses without EDTA in the packaging, and (3) silicone hydrogel lenses without EDTA versus hydrogel lenses with EDTA both in Smart Touch Technology packaging. Participants washed hands, underwent finger swabs, and inserted the lenses. After 45 minutes, lenses were removed aseptically and the posterior lens surfaces cultured.

Results: Thirty-eight subjects (average age, 30.9 ± 12.5 years) participated in this study. Overall, the level of back surface contamination was low for both lens materials, ranging from 0 to 43 colony-forming unit (CFU)/lens for the silicone hydrogel and 0 to 17 CFU/lens for the hydrogel lenses. The proportion of lenses with zero back surface contamination ranged from 16 to 64% for silicone hydrogel lenses and 28 to 64% for hydrogel lenses. Contact lenses from conventional packaging containing EDTA had 3.38 times increased risk (95% confidence interval [CI], 1.02 to 11.11; P = .05) of contamination being present compared with lenses from Smart Touch packaging with EDTA. Contact lenses from conventional packaging without EDTA had 3.4 times increased risk (95% CI, 1.02 to 11.36; P = .05) of contamination being present compared with Smart Touch packaging without EDTA, and silicone hydrogel lenses had a 6.28 times increased risk (95% CI, 1.65 to 23.81; P = .007) of contamination being present compared with hydrogels. The median (interquartile range) number of bacteria isolated from fingers used to perform lens insertion after handwashing but before lens insertion was not significantly different between the silicone hydrogel and hydrogel lenses (63.7 [204.2] vs. 59 [84.5], P = .09). Finger contamination was not significantly associated with lens contamination in the presence or absence of EDTA.

Conclusions: Smart Touch Technology packaging was effective in reducing the proportion of contaminated lenses. Although silicone hydrogel lenses were more likely to be contaminated, the presence of EDTA ameliorated this effect. Finger contamination was not associated with lens contamination.
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http://dx.doi.org/10.1097/OPX.0000000000001693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132605PMC
May 2021

A Programmed Cell-Mimicking Nanoparticle Driven by Potato Alkaloid for Targeted Cancer Chemoimmunotherapy.

Adv Healthc Mater 2021 May 8:e2100311. Epub 2021 May 8.

Medical Research Institute, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China.

Membrane camouflaged-nanoparticles (CM-NPs) have been exploited to inherit desired functionalities from source cells. Despite those advantages, membrane cloak may play a "double-edged sword" role in tumor-targeting therapy, as the intact membrane coating may hinder function-exertion of loaded drugs after reaching predetermined site. Therefore, further optimization of CM-NPs is still needed to enhance their delivery efficiency. Herein, natural product, Solamargine (SM), a cholesterol-affiliative amphiphilic potato alkaloid is first applied as core component of "inner core," to design a cell-mimicking "core-shell" nanoparticle (RBC-SLip) with acid-responsive off-coating properties for tumor-targeted therapy. Owing to red blood cell membrane (RBCm)-derived outer coating, it circulates stably in physiological conditions. While it would undergo an off-coating morphological change in response to acid stimuli in tumor microenvironment (TME), afterwards, the resulting off-coating liposome (SLip) shows active tumor-targeting and endosomal escape abilities, thus contributing to superior antitumor efficacy. In addition, SM also possesses natural TME-modulating ability; therefore, RBC-SLip can synergize with the PD1/PD-L1 blockade immunotherapy when encapsulated with PTX to achieve enhanced chemoimmunotherapy. The off-coating strategy developed by natural products SM, provide a brand-new perspective to optimize CM-NPs, and it also embodies application value of "unification of medicines and excipients" of natural products.
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http://dx.doi.org/10.1002/adhm.202100311DOI Listing
May 2021

Publisher Correction: Triacylglycerols are preferentially oxidized over free fatty acids in heated soybean oil.

NPJ Sci Food 2021 Apr 28;5(1):13. Epub 2021 Apr 28.

Department of Food Science and Technology, College of Agriculture and Environmental Sciences, University of California Davis, Davis, CA, USA.

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http://dx.doi.org/10.1038/s41538-021-00095-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080563PMC
April 2021

Nanoscale metal-organic frameworks for tumor phototherapy.

J Mater Chem B 2021 05;9(18):3756-3777

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University, Nanjing 210009, P. R. China.

Metal-Organic Frameworks (MOFs) are constructed from metal ions/cluster nodes and functional organic ligands through coordination bonds. Owing to the advantages of diverse synthetic methods, easy modification after synthesis, large adsorption capacity for heavy metals, and short equilibrium time, considerable attention has recently been paid to MOFs for tumor phototherapy. Through rational tuning of metal ions and ligands, MOFs present abundant properties for various applications. Light-triggered phototherapy, including photodynamic therapy (PDT) and photothermal therapy (PTT), is an emerging cancer treatment approach. Nanosized MOFs can be applied as phototherapeutic agents to accomplish phototherapy with excellent phototherapeutic efficacy. This review outlines the latest advances in the field of phototherapy with various metal ion-based MOFs.
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http://dx.doi.org/10.1039/d1tb00349fDOI Listing
May 2021

Triacylglycerols are preferentially oxidized over free fatty acids in heated soybean oil.

NPJ Sci Food 2021 Apr 1;5(1). Epub 2021 Apr 1.

Department of Food Science and Technology, College of Agriculture and Environmental Sciences, University of California Davis, Davis, CA, USA.

In oil, free fatty acids (FFAs) are thought compared the efficiency of hydrolysis wto be the preferred substrate for lipid oxidation although triacylglycerols (TAGs) are the predominant lipid class. We determined the preferential oxidation substrate (TAGs versus FFAs) in soybean oil heated at 100 °C for 24 h, after validating a method for quantifying esterified and free lipid oxidation products (i.e., oxylipins) with mass-spectrometry. Reaction velocities and turnover (velocity per unit substrate) of FFA, and free and TAG-bound (esterified) oxylipins were determined. FFA hydrolysis rate and turnover were orders of magnitude greater (16-4217 fold) than that of esterified and free oxylipin formation. The velocity and turnover of TAG-bound oxylipins was significantly greater than free oxylipins by 282- and 3-fold, respectively. The results suggest that during heating, TAGs are preferentially oxidized over FFAs, despite the rapid hydrolysis and availability of individual FFAs as substrates for oxidation. TAG-bound oxylipins may serve as better markers of lipid oxidation.
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http://dx.doi.org/10.1038/s41538-021-00086-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016982PMC
April 2021

Elegant Construction of ZnInS/BiVO Hierarchical Heterostructures as Direct Z-Scheme Photocatalysts for Efficient CO Photoreduction.

ACS Appl Mater Interfaces 2021 Apr 24;13(13):15092-15100. Epub 2021 Mar 24.

Key Laboratory of Modern Acoustics (MOE), Institute of Acoustics, School of Physics, National Laboratory of Solid State Microstructures, Collaborative Innovation Center of Advanced Microstructures, Nanjing University, Nanjing, Jiangsu 210093, P. R. China.

The ZnInS/BiVO heterostructures were elegantly designed through assembling ZnInS nanosheets onto the surface of BiVO decahedrons. This composite photocatalyst exhibits efficient photocatalytic conversion of CO into CO with a detectable amount of CH in the presence of water vapor. An electron spin-resonance spectroscopy (ESR) technique and density function theory (DFT) calculation affirm the direct Z-scheme structure in ZnInS/BiVO. The larger surface photovoltage (SPV) change and the longer liquid photoluminescence (PL) lifetime of the heterostructure, compared to the individual ZnInS and BiVO components, demonstrate that the Z-scheme structure can effectively promote the recombination of the photogenerated holes in the valence band (VB) of the ZnInS nanosheet with the electrons in the conduction band (CB) of the decahedral BiVO and lead to the abundant electrons surviving in the CB of ZnInS and holes in the VB of BiVO, thus enhancing photocatalytic CO reduction performance. This study may make a potential contribution to the rational construction and deep understanding of the underlying mechanism of direct Z-schemes for advanced photocatalytic activity.
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http://dx.doi.org/10.1021/acsami.0c21266DOI Listing
April 2021

Lipidomics phenotyping of clam (Corbicula fluminea) through graphene/fibrous silica nanohybrids based solid-phase extraction and HILIC-MS analysis.

Food Chem 2021 Aug 13;354:129565. Epub 2021 Mar 13.

Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China. Electronic address:

Polyunsaturated phospholipids are abundant in clam (Corbicula fluminea) but difficult to be fully extracted. Herein, graphene/fibrous silica (G/KCC-1) nanohybrids were synthesized, characterized, and applied for solid-phase extraction (SPE) of phospholipids in clam. The effectiveness of G/KCC-1 SPE was verified by hydrophilic interaction chromatography mass spectrometry (HILIC-MS) based lipidomics and statistical analysis. The ions of PE 16:0/18:1 (m/z 716.4), PC 16:0/20:5 (m/z 824.6) and etc. were regarded as the main difference among the crude lipids, acetone washed extract, and eluate of G/KCC-1 SPE. Finally, this method was validated in terms of linearity (R 0.9965 to 0.9981), sensitivity (LOD 0.19-0.51 μg·mL and LOQ 0.48 - 1.47 μg·mL), and precision (RSD ≤ 7.16% and RSD ≤ 7.30%). In conclusion, the G/KCC-1 SPE and HILIC-MS method was shown to be accurate and efficient in selective extracting and phenotyping phospholipids in C. fluminea.
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http://dx.doi.org/10.1016/j.foodchem.2021.129565DOI Listing
August 2021

Stability improvement of reduced-fat reduced-salt meat batter through modulation of secondary and tertiary protein structures by means of high pressure processing.

Meat Sci 2021 Jun 17;176:108439. Epub 2021 Jan 17.

Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, PR China.

This study investigated the effect of high-pressure processing (HPP) at 100 to 400 MPa for 2 min on the stability of reduced-fat reduced-salt (RFRS) meat batter. Total expressible fluid (TEF) of RFRS batter reached its minimum value at 200 MPa. The results of Raman spectra revealed that α-helix reached its random coils increased as the pressure level was increased, and pressure up to 200 MPa remarkably increased protein unfolding but 400 MPa increased aggregation. Finally, Raman spectra and magnetic resonance imaging (MRI) revealed that 200 MPa significantly increased tryptophan, tyrosine doublet, CH and/or CH stretching and proton intensities related to water and fats; but decreased β structures, SS stretching (475) and (g-g-t or t-g-t, 540), as compared with the control. RFRS batter treated at 200 MPa is beneficial for the meat industry from the technological point of view and for consumers from the health point of view, as the improved emulsion stability contributed by the modified secondary and tertiary structures.
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http://dx.doi.org/10.1016/j.meatsci.2021.108439DOI Listing
June 2021

Ferroptosis and microbial pathogenesis.

PLoS Pathog 2021 Mar 4;17(3):e1009298. Epub 2021 Mar 4.

Temasek Life Sciences Laboratory; and Department of Biological Sciences, National University of Singapore, Singapore.

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http://dx.doi.org/10.1371/journal.ppat.1009298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7932112PMC
March 2021

The regulation of sodium alginate on the stability of ovalbumin-pectin complexes for VD encapsulation and in vitro simulated gastrointestinal digestion study.

Food Res Int 2021 02 15;140:110011. Epub 2020 Dec 15.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, People's Republic of China. Electronic address:

The ovalbumin (OVA)-pectin (PEC)-sodium alginate (SA)-Vitamin D (VD) complex nanoparticles were fabricated by antisolvent precipitation method, and the excellent encapsulation efficiency and loading capacity of VD were obtained by 96.6% and 2.8%, respectively. Compared with ternary OVA-PEC-VD complexes, the addition of SA with strong negative charge effectively regulated the OVA-PEC complexes and significantly improved the stability of OVA-PEC-SA-VD complex nanoparticles with preferable size as small as 126 nm. The storage stability was also investigated after low temperature storage for 31 d, and the particle size of quaternary complexes was increased only 40 nm. In vitro digestion results elucidated that the complex nanoparticles had good stability in the simulated gastric fluid, and almost completely released in the simulated intestinal fluid confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) experiments and scanning electron microscope (SEM) images. The release kinetics study clarified that it was close to Fick release. Fluorescence and Fourier transform infrared spectroscopy (FTIR) experiments showed that quaternary complex nanoparticles were mainly combined by electrostatic, hydrophobic and hydrogen bonding interactions. The novel quaternary protein-polysaccharide complexes have excellent stability and great sustained-release performance for VD, which may be helpful for the digestion and absorption of vitamin by human body, thus have potential applications in the food and drug industry.
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http://dx.doi.org/10.1016/j.foodres.2020.110011DOI Listing
February 2021

Modeling of Nucleation and Growth in the Synthesis of PbS Colloidal Quantum Dots Under Variable Temperatures.

ACS Omega 2021 Feb 29;6(5):3701-3710. Epub 2021 Jan 29.

Faculty of Informatics and Engineering, The University of Electro-Communications, Chofu, Tokyo 182-8585, Japan.

Lead sulfur colloidal quantum dots (PbS CQDs) are a kind of IV-VI semiconductor nanocrystals which have attracted enormous interest in recent years because of their unique physicochemical properties. Controlling size, size distribution, and yield of PbS CQDs plays key priorities in order to improve their properties when they are applied in the photovoltaics and energy storage applications. Despite many systematical studies in PbS CQD syntheses with various perspectives, details of the formation mechanism impacted on the size, concentration, and size distribution of PbS CQDs in complicated reaction conditions remain poorly understood. In this work, an improved kinetic rate equation (IKRE) model is employed to describe PbS CQD formation under variable solution temperatures. After establishing the necessary discretized equations and reviewing the link between model parameters and experimental information, a parametric study is performed to explore the model's feature. In addition, a set of experimental data has been compared with the result of IKRE model fits, which would be used to obtain corresponding thermodynamic and kinetic parameters that can further affect the CQD growth over longer timescales. This method builds up the relationship between the nucleation and Ostwald ripening stage that would provide the possibility for future large-scale manufacturing of CQDs.
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http://dx.doi.org/10.1021/acsomega.0c05223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876681PMC
February 2021

Psychiatric Disorders and Cardiovascular Diseases During the Diagnostic Workup of Suspected Prostate Cancer.

JNCI Cancer Spectr 2021 Feb 7;5(1):pkaa108. Epub 2020 Nov 7.

Unit of Integrative Epidemiology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.

Background: It is unknown whether the rate of psychiatric disorders and cardiovascular disease increases during the diagnostic workup of suspected prostate cancer.

Methods: We designed a population-based cohort study including 579 992 men living during 2005-2014 in Skåne, Sweden, according to the Swedish Total Population Register and the Skåne Healthcare Register (SHR). We used the Swedish Cancer Register and the SHR to identify all men with a new diagnosis of prostate cancer (N = 10 996), and all men underwent a prostate biopsy without receiving a cancer diagnosis (biopsy group, N = 20 482) as exposed to a diagnostic workup. Using Poisson regression, we compared the rates of psychiatric disorders and cardiovascular disease during the period before diagnosis or biopsy of exposed men with the corresponding rates of unexposed men.

Results: We found an increased rate of psychiatric disorders during the period before diagnosis or biopsy among men with prostate cancer (incidence rate ratio [IRR] = 1.87, 95% confidence interval [CI] = 1.67 to 2.10) and men in the biopsy group (IRR = 2.22, 95% CI = 2.08 to 2.37). The rate of cardiovascular disease increased during the period before diagnosis or biopsy among men with prostate cancer (IRR = 2.22, 95% CI = 2.12 to 2.32) and men in the biopsy group (IRR = 2.56, 95% CI = 2.49 to 2.63). Greater rate increases were noted for a diagnostic workup due to symptoms than due to other reasons.

Conclusions: There was an increased risk of psychiatric disorders and cardiovascular disease during the diagnostic workup of suspected prostate cancer regardless of the final cancer diagnosis.
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http://dx.doi.org/10.1093/jncics/pkaa108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853179PMC
February 2021

MicroRNA-23a reduces lipopolysaccharide-induced cellular apoptosis and inflammatory cytokine production through Rho-associated kinase 1/sirtuin-1/nuclear factor-kappa B crosstalk.

Chin Med J (Engl) 2021 Feb 3;134(7):829-839. Epub 2021 Feb 3.

Department of Emergency Medicine, South Campus of the Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 201499, China.

Background: MicroRNAs are closely associated with the progression and outcomes of multiple human diseases, including sepsis. In this study, we examined the role of miR-23a in septic injury.

Methods: Lipopolysaccharide (LPS) was used to induce sepsis in a rat model and H9C2 and HK-2 cells. miR-23a expression was evaluated in rat myocardial and kidney tissues, as well as H9C2 and HK-2 cells. A miR-23a mimic was introduced into cells to identify the role of miR-23a in cell viability, apoptosis, and the secretion of inflammatory cytokines. Furthermore, the effect of Rho-associated kinase 1 (ROCK1), a miR-23a target, on cell damage was evaluated, and molecules involved in the underlying mechanism were identified.

Results: In the rat model, miR-23a was poorly expressed in myocardial (sham vs. sepsis 1.00 ± 0.06 vs. 0.27 ± 0.03, P < 0.01) and kidney tissues (sham vs. sepsis 0.27 ± 0.03 vs. 1.00 ± 0.06, P < 0.01). Artificial overexpression of miR-23a resulted in increased proliferative activity (DNA replication rate: Control vs. LPS vs. LPS + Mock vs. LPS + miR-23a: H9C2 cells: 34.13 ± 3.12 vs. 12.94 ± 1.21 vs. 13.31 ± 1.43 vs. 22.94 ± 2.26, P < 0.05; HK-2 cells: 15.17 ± 1.43 vs. 34.52 ± 3.46 vs. 35.19 ± 3.12 vs. 19.87 ± 1.52, P < 0.05), decreased cell apoptosis (Control vs. LPS vs. LPS + Mock vs. LPS + miR-23a: H9C2 cells: 11.39 ± 1.04 vs. 32.57 ± 2.29 vs. 33.08 ± 3.12 vs. 21.63 ± 2.35, P < 0.05; HK-2 cells: 15.17 ± 1.43 vs. 34.52 ± 3.46 vs. 35.19 ± 3.12 vs. 19.87 ± 1.52, P < 0.05), and decreased production of inflammatory cytokines, including interleukin-6 (Control vs. LPS vs. LPS + Mock vs. LPS + miR-23a: H9C2 cells: 59.61 ± 5.14 vs. 113.54 ± 12.30 vs. 116.51 ± 10.69 vs. 87.69 ± 2.97 ng/mL; P < 0.05, F = 12.67, HK-2 cells: 68.12 ± 6.44 vs. 139.65 ± 16.62 vs. 143.51 ± 13.64 vs. 100.82 ± 9.74 ng/mL, P < 0.05, F = 9.83) and tumor necrosis factor-α (Control vs. LPS vs. LPS + Mock vs. LPS + miR-23a: H9C2 cells: 103.20 ± 10.31 vs. 169.67 ± 18.84 vs. 173.61 ± 15.91 vs. 133.36 ± 12.32 ng/mL, P < 0.05, F = 12.67, HK-2 cells: 132.51 ± 13.37 vs. 187.47 ± 16.74 vs. 143.51 ± 13.64 vs. 155.79 ± 15.31 ng/mL, P < 0.05, F = 9.83) in cells. However, ROCK1 was identified as a miR-23a target, and further up-regulation of ROCK1 mitigated the protective function of miR-23a in LPS-treated H9C2 and HK-2 cells. Moreover, ROCK1 suppressed sirtuin-1 (SIRT1) expression to promote the phosphorylation of nuclear factor-kappa B (NF-κB) p65, indicating the possible involvement of this signaling pathway in miR-23a-mediated events.

Conclusion: Our results indicate that miR-23a could suppress LPS-induced cell damage and inflammatory cytokine secretion by binding to ROCK1, mediated through the potential participation of the SIRT1/NF-κB signaling pathway.
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http://dx.doi.org/10.1097/CM9.0000000000001369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104237PMC
February 2021

Structural basis for high-pressure improvement in depolymerization of interfacial protein from RFRS meat batters in relation to their solubility.

Food Res Int 2021 01 3;139:109834. Epub 2020 Nov 3.

Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, PR China. Electronic address:

High-pressure processing (HPP) can modify the construction of interfacial proteins (IPs) to improve the properties of reduced-fat and reduced-salt (RFRS) meat batters. In this study, the relationship between the construction of IPs and their solubility at fat droplet/water interface in RFRS meat batters with HPP treatments was investigated. When 200 MPa for 2 min was applied, the IPs exhibited the highest solubility due to a high concentration of absorbed myosin with the content of random coil 65.62%, but the particle diameter was in reverse. The microscopy revealed the depolymerization of IPs occurred at low pressure, while macromolecular aggregates were produced as the cross-linking of IPs to some degree at pressure ≥ 200 MPa. This phenomenon was supported by the result of SDS-PAGE and the sulfhydryl of IPs. In conclusion, the HPP induced solubility alteration of IPs was achieved by modifying their construction through adjusting the secondary structures and regulating bond interactions.
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http://dx.doi.org/10.1016/j.foodres.2020.109834DOI Listing
January 2021

Identification and construction of a novel biomimetic delivery system of paclitaxel and its targeting therapy for cancer.

Signal Transduct Target Ther 2021 Jan 27;6(1):33. Epub 2021 Jan 27.

Institute of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai, 200041, China.

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http://dx.doi.org/10.1038/s41392-020-00390-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840929PMC
January 2021

Effect of steam explosion pretreatment on the production of microscale tuna bone power by ultra-speed pulverization.

Food Chem 2021 Jun 8;347:129011. Epub 2021 Jan 8.

Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China; Zhejiang Marine Development Research Institute, Zhoushan, China. Electronic address:

In this study, a steam explosion pretreatment method was established to prepare tuna bone powder. The conditions were optimized such that steam pressure of 0.6 MPa, reaction time of 5 min, and sample weight of 100 g. The result showed that steam explosion pretreatment would not change the chemical structure of bone powder, however, the median particle size (D) of the steam explosion pretreated tuna bone powder (SE-TBP) (13.186 μm) was significantly smaller than that of normal biological calcium tuna bone powder (N-TBP) (169.762 μm). The calcium absorption rate (79.75 ± 2.33%) and utilization rate (78.75% ± 2.85%) of the mice fed with SE-TBP were both higher than those of fed with CaCO or N-TBP with the same calcium equivalent in the feed. The steam explosion pretreatment method could obtain ideal tuna bone powder in a shorter time, provide a method for deep processing and utilization of tuna bone by-product.
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http://dx.doi.org/10.1016/j.foodchem.2021.129011DOI Listing
June 2021

Real-Time Screening of Omega-7 Phospholipids in Marine Biological Resources Using an iKnife-Rapid-Evaporative-Ionization-Mass-Spectrometry-Based Lipidomics Phenotype.

J Agric Food Chem 2021 Jan 12. Epub 2021 Jan 12.

Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, Zhejiang 310012, People's Republic of China.

Omega-7 (n-7) phospholipids were bioactive substances in marine animals. In this study, a fast lipidomics phenotyping approach for real-time screening of n-7 phospholipids in five kinds of economic seafood, salmon, prawn, bluefin tuna, hairtail, and butterfish, was established using iKnife rapid evaporative ionization mass spectrometry (REIMS). The n-7 phospholipids were structurally characterized and quantitatively analyzed, and the profiles were statistically analyzed by multivariate recognition analysis. It indicated that the difference of n-7 phospholipids in seafood samples was significant ( < 0.05), with (cum) and (cum) values of >0.9. The proportion of n-7 phospholipids in salmon was the highest (20.43%), followed by bluefin tuna, prawn, hairtail, and butterfish. The ions of / 742.54 (PC 16:1-18:1), 768.55 (PC 16:1-20:2), 697.48 (PE 16:1-18:1), and 699.48 (PE 16:1-18:0) were the main n-7 phospholipids. The effectiveness of iKnife REIMS was further verified by hydrophilic interaction chromatography mass spectrometry and gas chromatography. The results demonstrated that proposed iKnife REIMS was an excellent technique for front-line screening of n-7 phospholipids in a large variety of marine biological resources.
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http://dx.doi.org/10.1021/acs.jafc.0c05442DOI Listing
January 2021

Titania-coated fibrous silica (TiO/KCC-1) core-shell microspheres based solid-phase extraction in clam (Corbicula fluminea) using hydrophilic interaction liquid chromatography and mass spectrometry.

Food Res Int 2020 11 8;137:109408. Epub 2020 Jun 8.

Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China. Electronic address:

Clam (Corbicula fluminea) contains significant amount of phospholipids, and the profile of phospholipids could be used as an index for nutrition evaluation. In this study, a titania-coated fibrous silica (TiO/KCC-1) core-shell microsphere based solid-phase extraction (SPE) and hydrophilic interaction liquid chromatography mass spectrometry (HILIC-MS) method was developed to study the phospholipids in clam. The structure and morphology of TiO/KCC-1 were characterized by transmission electron microscopy (TEM), scanning electron microscope (SEM), X-ray diffraction (XRD), and ultraviolet-visible diffuse reflectance spectroscopy (UV-vis DRS). The analytical results indicated that the phospholipid molecular species (PMS) could be extracted in a cleaner manner after TiO/KCC-1 SPE, benefiting from the enhanced signals and decreased MS noise of phospholipids. Based on the principal component analysis (PCA) and partial least squares regression discriminant analysis (PLS-DA) models, the PMS with the most obvious beneficiaries of TiO/KCC-1 SPE were identified, e.g. PC 16:0/20:5 (m/z 824.6), PC 16:0/22:6 (m/z 850.6), PE 18:1/18:2 (m/z 740.4), and PE 18:0/22:6 (m/z 790.5). Finally, the proposed method was validated to be sensitive and precise. This study provides an efficient tool to analyze the phospholipids in clam and the prospect to use this method for future applications in various samples.
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http://dx.doi.org/10.1016/j.foodres.2020.109408DOI Listing
November 2020

Untargeted Screening of EPA/DHA Structured Phospholipids in Krill Oil by Chain-Lock-Driven Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry.

J Agric Food Chem 2020 Dec 23;68(49):14652-14659. Epub 2020 Nov 23.

Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, 310013, China.

Eicosapentaenoic and docosahexaenoic acids structured phospholipids (PL) have multiple biochemical and pharmacological effects on human health. In this study, EPA and DHA chains were locked under precursor ion scan (PreIS) mode for untargeted screening PL in krill oil using hydrophilic interaction liquid chromatography tandem mass spectrometry (HILIC-MS/MS). The effect of collision energy and declustering potential on the fragmentation of EPA ( 301.2) and DHA ( 327.2) chains was studied. A total of 33 PL were characterized (sn-1/sn-2) and quantified using regression models, including 16 PC, 11 PE, and 6 PI. Afterward, this method was validated in terms of linearity (≥0.9978), sensitivity (LOD ≤ 4.02 μg·L), precision (RSD ≤ 4.71%), and recovery (≥78.9%). Finally, the performance of HILIC-PreIS-MS/MS was compared with those of conventional methods, and the results indicated its superiority in selective screening PL in krill oil.
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http://dx.doi.org/10.1021/acs.jafc.0c06675DOI Listing
December 2020

Monodisperse microsphere-based immobilized metal affinity chromatography approach for preparing Antarctic krill phospholipids followed by HILIC-MS analysis.

Food Chem 2021 May 9;344:128585. Epub 2020 Nov 9.

Zhejiang Province Key Laboratory of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China. Electronic address:

Phospholipids enriched krill is a functional food beneficial in cardiovascular diseases. Herein, monodisperse microsphere-based immobilized metal affinity chromatographic material (MM-IMAC) was synthesized with Ti incorporated to enrich phospholipids from krill by coordination with phosphate group. The extract was profiled by hydrophilic interaction chromatography-mass spectrometry (HILIC-MS) with 154 phospholipid molecular species detected. The parameters were loading solvent n-hexane/isopropanol (2:8, v/v), flow rate 0.8 mL·min, and eluting volume 1 mL. Besides, eicosapentaenoic and docosahexaenoic acids structured phospholipids were located, such as phosphatidylcholine (PC) 20:5/22:6, phosphatidylinositol (PI) 18:0/20:5, etc. Finally, this method was validated in linearity (R ≥ 0.9953), sensitivity (LOD ≤ 0.53 μg·mL and LOQ ≤ 1.66 μg·mL), precision (RSD ≤ 4.86% and RSD ≤ 6.25%), and recovery (58-83%). It indicated that the MM-Ti-IMAC-HILIC-MS was reliable and efficient in specific study of phospholipids in food matrix.
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http://dx.doi.org/10.1016/j.foodchem.2020.128585DOI Listing
May 2021

Combined Spiral Transformation and Model-Driven Multi-Modal Deep Learning Scheme for Automatic Prediction of TP53 Mutation in Pancreatic Cancer.

IEEE Trans Med Imaging 2021 Feb 2;40(2):735-747. Epub 2021 Feb 2.

Pancreatic cancer is a malignant form of cancer with one of the worst prognoses. The poor prognosis and resistance to therapeutic modalities have been linked to TP53 mutation. Pathological examinations, such as biopsies, cannot be frequently performed in clinical practice; therefore, noninvasive and reproducible methods are desired. However, automatic prediction methods based on imaging have drawbacks such as poor 3D information utilization, small sample size, and ineffectiveness multi-modal fusion. In this study, we proposed a model-driven multi-modal deep learning scheme to overcome these challenges. A spiral transformation algorithm was developed to obtain 2D images from 3D data, with the transformed image inheriting and retaining the spatial correlation of the original texture and edge information. The spiral transformation could be used to effectively apply the 3D information with less computational resources and conveniently augment the data size with high quality. Moreover, model-driven items were designed to introduce prior knowledge in the deep learning framework for multi-modal fusion. The model-driven strategy and spiral transformation-based data augmentation can improve the performance of the small sample size. A bilinear pooling module was introduced to improve the performance of fine-grained prediction. The experimental results show that the proposed model gives the desired performance in predicting TP53 mutation in pancreatic cancer, providing a new approach for noninvasive gene prediction. The proposed methodologies of spiral transformation and model-driven deep learning can also be used for the artificial intelligence community dealing with oncological applications. Our source codes with a demon will be released at https://github.com/SJTUBME-QianLab/SpiralTransform.
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http://dx.doi.org/10.1109/TMI.2020.3035789DOI Listing
February 2021

Risk of Injuries around Diagnosis of Cervical Cancer and Its Precursor Lesions: A Nationwide Cohort Study in Sweden.

Cancer Epidemiol Biomarkers Prev 2020 11 21;29(11):2230-2234. Epub 2020 Oct 21.

Institutet of Environmental Medicine, Karolinska Institutet, Solna, Stockholm, Sweden.

Background: Highly increased risk of injuries has been noted around the time of cancer diagnosis. Whether there is a similar increase in risk around the diagnosis of cervical cancer and its precursor lesions was unknown.

Methods: We performed a cohort study including 3,016,307 Swedish women that participated in cervical screening during 2001 to 2012. We calculated the incidence rates (IR) of hospitalized iatrogenic or noniatrogenic injuries during the diagnostic workup, and the time interval from smear or punch biopsy until surgical treatment or 2 months after the last smear or biopsy, among women with invasive cervical cancer (ICC) or its precursor lesions. We calculated the IRs of injuries during the 2 months after a normal smear among the other women as reference. IR ratios (IRR) and 95% confidence intervals (CI) were calculated using Poisson regression.

Results: Compared with other women, there was an increased rate of iatrogenic injuries during the diagnostic workup of women with ICC (IR, 0.58 per 1,000 person-months; IRR, 8.55; 95% CI, 3.69-19.80) as well as of women with cervical intraepithelial neoplasia grade 3 and adenocarcinoma (IR, 0.09 per 1,000 person-months; IRR, 3.04; 95% CI, 1.73-5.34). We also found an increased rate of noniatrogenic injuries during the diagnostic workup of women with invasive cancer (IR, 0.65 per 1,000 person-months; IRR, 2.48; 95% CI, 1.30-4.47).

Conclusions: Although rare, there was an increased risk of inpatient care for iatrogenic and noniatrogenic injuries during the diagnostic workup of women with ICC.

Impact: Women experienced burden of medical complications and psychologic distress around diagnosis of a potential cervical cancer.
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http://dx.doi.org/10.1158/1055-9965.EPI-20-0673DOI Listing
November 2020

QuEChERS and 96-well plate solid phase extraction for determination of vancomycin and norvancomycin in fish meat by UPLC-MS/MS.

Food Chem 2021 Apr 10;342:128326. Epub 2020 Oct 10.

Zhoushan Institute for Food and Drug Control, Zhoushan 316000, China. Electronic address:

Vancomycin and norvancomycin are glycopeptide antibiotics for gram-positive bacteria infection, but indiscriminately used in aquaculture. In this study, a QuEChERS (quick, easy, cheap, effective, rugged, and safe)/96-well solid-phase extraction (SPE) plate method was used to extract vancomycin and norvancomycin in fish meat samples, and the drugs were further analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The parameters, such as the sorbent of cation exchange resin, the proportion of acetonitrile (15%) in extractant, the mobile phase of water (0.1% formic acid)/acetonitrile, were optimized. The method was validated in terms of linearity (0.9990-0.9994), LOD (0.51 μg·kg), LOQ (1.73 μg·kg), intra-dayprecision (<5.19%), inter-day precision (<6.30%), and recovery (86.7-98.6%). Finally, the method was successfully applied to contaminated and randomly collected samples. The results indicated that the proposed method meet the daily monitoring requirements for vancomycin and norvancomycin.
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http://dx.doi.org/10.1016/j.foodchem.2020.128326DOI Listing
April 2021