Publications by authors named "Qing Chen"

1,344 Publications

  • Page 1 of 1

Effects of curcumin on the bioavailability of dioxin-like pollutants in rats.

Sci Rep 2021 Jun 14;11(1):12461. Epub 2021 Jun 14.

Zhejiang Provincial Center for Disease Control and Prevention, Binsheng Road 3399, Hangzhou, 310051, China.

The effects of curcumin on the bioavailability of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) were investigated in Sprague-Dawley rats. Tetra- and penta-chlorinated PCDFs had the lowest bioavailability and hexa-chlorinated PCDD/Fs had the highest, while there was no obvious change in that of DL-PCBs. Curcumin markedly reduced the toxic equivalent (TEQ) of PCDD/Fs in rats, illustrating the potential to competitively inhibit absorption of PCDD/Fs by the epithelial cells of the small intestine due to the similar chemical structure (diphenyl) between curcumin and PCDD/Fs. Moreover, curcumin lowered the TEQ of DL-PCBs in the liver of male rats, but not female rats. The significant decrease in the bioavailability of PCDD/Fs and DL-PCBs demonstrates the potential detoxification mechanisms of curcumin.
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http://dx.doi.org/10.1038/s41598-021-92085-3DOI Listing
June 2021

Efficacy and Safety of Endovascular Treatment for Acute Large-Vessel Ischemic Stroke Beyond 6 h After Symptom Onset: A Meta-Analysis.

Front Neurol 2021 28;12:654816. Epub 2021 May 28.

Department of Neurosurgery, Fujian Medical University Union Hospital, Fuzhou, China.

There is considerable evidence on the benefits of endovascular thrombectomy (EVT) for acute ischemic stroke (AIS) within 6 h after symptom onset. However, uncertainties remain regarding EVT efficacy beyond 6 h after symptom onset. We undertook a meta-analysis to assess the efficacy and safety of EVT in patients with AIS >6 h after symptom onset. We searched PubMed, EMBASE, and Chinese Biomedical through July 2019. We included studies involving early (≤6 h) vs. delayed (>6 h) EVT in selected patients with AIS, based on radiological evaluation criteria. Functional independence, successful recanalization, mortality, and symptomatic intracranial hemorrhage (sICH) rates were assessed. Eight articles, with 3,265 patients who had undergone early EVT and 1,078 patients who had received delayed EVT, were included in the meta-analysis. Patients treated with early EVT showed a similar proportion of functional independence at 90 days [odds ratio (OR) = 1.14, 95% confidence interval (CI) = 0.926-1.397, = 0.219; = 36.2%, = 0.128] as those treated with delayed EVT. Delayed EVT was also associated with no significant difference in mortality (OR = 1.015, 95% CI = 0.852-1.209; = 0.871; = 0.0%, = 0.527), successful recanalization (OR = 1.255, 95% CI = 0.923-1.705; = 0.147; = 60.5%, = 0.009), and sICH (OR = 0.976, 95% CI = 0.737-1.293; = 0.871; = 0.0%, = 0.742) rates compared with early EVT. Among selected patients with AIS, delayed EVT showed comparable outcomes in functional independence, recanalization, mortality, and sICH rates compared with early EVT.
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http://dx.doi.org/10.3389/fneur.2021.654816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195613PMC
May 2021

RELT promotes the growth of esophageal squamous cell carcinoma by activating the NF-κB pathway.

Cell Cycle 2021 Jun 13:1-11. Epub 2021 Jun 13.

Department of Thoracic Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan, China.

The members of the tumor necrosis factor receptor (TNFR) family have been demonstrated to play critical roles in various cancers. However, little is known about the function of the Receptor Expressed in Lymphoid Tissues (RELT) in cancers, which is a member of the TNFR family, especially in the esophageal squamous cell carcinoma (ESCC). In this study, we found that RELT expression was increased in ESCC tissues and was consequently associated with poor overall survival of ESCC patients. Moreover, RELT overexpression was found to promote cell growth, cell cycle progression, and suppressed cell apoptosis ; it also decreased the expression of p27 and caspase 3, and increased the expression of survivin. In addition, RELT contributed to the tumorigenesis of ESCC . Furthermore, we suggest that RELT may function in the pathogenesis of ESCC by activating the nuclear factor κB (NF-κB) pathway. An inhibitor of NF-κB reversed the RELT-induced malignancy in the ESCC cells. Altogether, our findings identified that RELT served as an oncogene in ESCC through the NF-κB pathway, suggesting that RELT may be developed as a novel biomarker for the diagnosis and treatment of the ESCC.
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http://dx.doi.org/10.1080/15384101.2021.1924451DOI Listing
June 2021

A prediction score model and survival analysis of acute kidney injury following orthotopic liver transplantation in adults.

Ann Palliat Med 2021 May 24. Epub 2021 May 24.

Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Acute kidney injury (AKI) is one of the postoperative complications following orthotopic liver transplantation (OLT), and is related to the high morbidity and mortality. Although there were numerous propensity factors for AKI, their cumulative influence remains unclear. Our aims were to develop a score model to predict postoperative AKI and to evaluate the impact of AKI on the recipients' long-term survival.

Methods: We retrospectively analyzed 99 adult patients underwent OLT in Union Hospital, Tongji Medical College, Huazhong University of Science and Technology between October 2014 and July 2020. The patients were dichotomized into the non-AKI and the AKI groups according to the Kidney Disease Improving Global Outcomes (KDIGO) criteria. We defined stage-1 AKI as mild AKI, stage-2 AKI and stage-3 AKI as severe AKI.

Results: Overall, 29 (29.29%) patients developed AKI after OLT, of these, stage-1, stage-2, stage-3 account for 20.20% (20 of 99 patients), 2.02% (2 of 99 patients), 7.07% (7 of 99 patients), respectively; and 13.79% of postoperative AKI patients (4 of 29 patients) accepted renal replacement therapy (RRT). Operative time and MELD-Na score predicted the postoperative AKI, with odds ratio of 1.006, 1.061, respectively. The generated AKI prediction model is as follows: -5.594+0.007×Operative time+0.060×MELD-Na. The area under the receiver operating characteristic curve (AUC) for the AKI prediction model was 0.762, and the sensitivity and specificity were 79.3%, 61.4%, respectively. There was no difference in long-term survival among the mild AKI group and the non-AKI group (P=0.751). However, the impact of severe AKI on longterm survival of patients was statistically significant when comparing the non-AKI group and the mild AKI group (P=0.001, P=0.011).

Conclusions: AKI occurs frequently in adult patients after OLT, and it poses a threat to patients' longterm survival. The severe AKI has negative impact on long-term survival, while the mild AKI has limited impact on long-term survival, compared with non-AKI group. The novel AKI prediction model has prognostic value in identifying patients at high risk for postoperative AKI.
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http://dx.doi.org/10.21037/apm-21-842DOI Listing
May 2021

Integrative Analysis of Genome, 3D Genome, and Transcriptome Alterations of Clinical Lung Cancer Samples.

Genomics Proteomics Bioinformatics 2021 Jun 8. Epub 2021 Jun 8.

Center for Bioinformatics, School of Life Sciences, Center for Statistical Science, Peking University, Beijing 100871, China. Electronic address:

Genomic studies of cancer cell alterations, such as mutations, copy number variations (CNVs), and translocations, greatly promote our understanding of the genesis and development of cancer. However, the 3D genome architecture of cancers remains less studied due to the complexity of cancer genomes and technical difficulties. To explore the 3D genome structure in clinical lung cancer, we performed Hi-C experiments using paired normal and tumor cells harvested from patients with lung cancer, combining with RNA-seq analysis. We demonstrated the feasibility of studying 3D genome of clinical lung cancer samples with a small number of cells (1 × 10), compared the genome architecture between clinical samples and cell lines of lung cancer, and identified conserved and changed spatial chromatin structures between normal and cancer samples. We also showed that Hi-C data can be used to infer CNVs and point mutations in cancer. By integrating those different types of cancer alterations, we showed significant associations between CNVs, 3D genome, and gene expression. We propose that 3D genome mediates the effects of cancer genomic alterations on gene expression through altering regulatory chromatin structures. Our study highlights the importance of analyzing 3D genomes of clinical cancer samples in addition to cancer cell lines and provides an integrative genomic analysis pipeline for future larger-scale studies in lung cancer and other cancers.
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http://dx.doi.org/10.1016/j.gpb.2020.05.007DOI Listing
June 2021

The Impact of hyperuricemia on long-term clinical outcomes of renal transplant recipients: a systematic review and meta-analysis.

J Pharm Pharm Sci 2021 ;24:292-307

Purpose: To evaluate the effect of hyperuricemia on clinical outcomes of renal transplant recipients (RTRs).

Methods: A literature search of PubMed, Cochrane, Embase was conducted up to March 20, 2020. The primary outcome was the estimated glomerular filtration rate (eGFR). The second outcomes were the risk of graft loss, death, cardiovascular event and the level of triglyceride. The following search terms were utilized: ((Hyperuricemic group) OR (Hyperuricaemia) OR (Hyperuric) OR (Urea acid) OR (Uric acid) OR (Acid urate) OR (Urate) OR (Gout)) and ((Transplantation) OR (Transplantations) OR (Transplant) OR (Transplants) OR (Graft)).

Results: 28 studies with 18224 patients were eligible for inclusion. There was no significant difference in eGFR (<12 months, p=0.07), the risk of graft loss (<60 months, p=0.07) and death (<60months, p=0.19) between the hyperuricemic and normouricemic group in the early post-transplantation period. But increased uric acid levels contributed to the long-term decline of eGFR, the risk of graft loss and death increased after transplantation. Hyperuricemia increased the risk of cardiovascular event with no significant difference in the level of triglyceride between the two groups.

Conclusions: Increased uric acid levels contributed to the long-term decline of eGFR, increased risk of graft loss and death after transplantation. Although there was no significant effect on triglyceride, hyperuricemia increased the risk of cardiovascular event.
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http://dx.doi.org/10.18433/jpps31620DOI Listing
January 2021

[Study on the Identification of Samples Mistyped as O Phenotype and the Strategy of Safe Blood Transfusion].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):910-916

Jiangsu Province Blood Center, Nanjing Medical University, Nanjing 210042, Jiangsu Province, China,Center for Global Health, Nanjing Medical University, Nanjing 210042, Jiangsu Province,

Objective: To investigate the indentification method of samples mistyped as O phenotype and to explore the precision transfusion strategy.

Methods: The blood samples from donors and patients admitted in our center from 2018 to 2019 was collected. The samples with O phenotype suspected subtypes were further determined by tube test, adsorption-elution test, etc. Molecular testing was used to sequence the related blood type genes of the subjects.

Results: Among 14 subjects misjudged as O, 11 different genotypes were identified, in which 3 blood donors were Ael02/O02, Bel03/O02, and one para-Bombay with B101/O02 (FUT1: h3h3; FUT2: SeSe); the genotypes of 11 patients were Ael02/O01, 2 cases with Ael02/O02, Ael08/O01, Aw37/O02, Aw43/O02, Bel03/O01, 3 cases with Bel03/O02, and one case was para-Bombay with A102/B101 (FUT1: h3h3; FUT2: SeSe).

Conclusion: The phenotypes of Ael, Bel, Aw and para-Bombay subtypes are easily misjudged as type O. Molecular technology is helpful to identify the genotype of subtypes, and the corresponding transfusion strategies could be reasonably performed.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.03.041DOI Listing
June 2021

Percutaneous coronary intervention versus coronary artery bypass grafting in patients with coronary heart disease and type 2 diabetes mellitus: Cumulative meta-analysis.

Clin Cardiol 2021 Jun 5. Epub 2021 Jun 5.

Department of Cardiology, Zhuzhou Central Hospital, Zhuzhou, China.

Previous meta-analyses showed that coronary artery bypass grafting (CABG) has lower all-cause mortality than percutaneous coronary intervention (PCI) for the management of coronary heart disease (CHD), but the long-term outcomes were not analyzed thoroughly in patients with type 2 diabetes mellitus (T2DM). To perform a meta-analysis of randomized controlled trials (RCTs) to explore the long-term effectiveness between CABG and PCI in patients with T2DM and study the temporal trends using a cumulative meta-analysis. PubMed, Embase, Cochrane library, and Clinical Trials Registry for eligible RCTs published up to September 2020. The outcomes were all-cause death, cardiac death, myocardial infarction, repeat revascularization, and stroke. Nine RCTs and 4566 patients were included. CABG resulted in better outcomes than PCI in terms of all-cause death (RR = 1.41, 95%CI: 1.22-1.63, p < 0.001), cardiac death (RR = 1.56, 95%CI: 1.25-1.95, p < 0.001), and repeat revascularization (RR = 2.68, 95%CI: 1.86-3.85, p < 0.001), but with difference regarding the occurrence of myocardial infarction (RR = 1.20, 95%CI: 0.78-1.85, p = 0.414), while PCI was associated with better outcomes in terms of stroke occurrence (RR = 0.51, 95%CI: 0.34-0.77, p = 0.001). The cumulative meta-analysis for all-cause death showed that the differences between CABG and PCI started to be significant at 3 years of follow-up, while the difference became significant at 5 years for cardiac death. In patients with CHD and T2DM, CABG results in better outcomes than PCI in terms of all-cause death, cardiac mortality, and repeat revascularization, while PCI had better outcomes in terms of stroke. The differences are mainly observed over the long-term follow-up.
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http://dx.doi.org/10.1002/clc.23613DOI Listing
June 2021

MiR-146a Negatively Regulates Aspergillus fumigatus-Induced TNF-α and IL-6 Secretion in THP-1 Macrophages.

Mycopathologia 2021 Jun 4. Epub 2021 Jun 4.

Jiangsu Key Laboratory of Molecular Biology for Skin, Institute of Dermatology, Diseases and STIs, Chinese Academy of Medical Science & Peking Union Medical College, Nanjing, 210042, People's Republic of China.

Aspergillus fumigatu (A. fumigatus) is one of the most common important fungal pathogens that cause life-threatening infectious disease in immunocompromised individuals. However, the host immune response against this pathogenic mold is not fully understood. MicroRNAs (miRNAs) play essential roles in regulating innate immunity. Thus, we investigated the function of miR-146a in inflammatory responses in macrophages after A. fumigatus stimulation in this study. We found that TNF-α and IL-6 were increased in THP-1 macrophage-like cells treated with A. fumigatus at both the mRNA and protein levels. The interaction between THP-1 macrophage-like cells and A. fumigatus resulted in a long-lasting increase in miR-146a expression dependent on p38 MAPK and NF-κB signaling. In A. fumigatus-challenged THP-1 macrophage-like cells, overexpression of miR-146a by miR-146a mimics decreased TNF-α and IL-6 production, whereas downregulation of miR-146a by anti-miR-146a significantly enhanced the level of TNF-α and IL-6. Our study demonstrates that the crosstalk between miR-146a and the inflammation-regulating p38 MAPK and NF-κB pathways might be a fine-tuning mechanism in the modulation of the inflammatory response in macrophages infected with A. fumigatus. Our findings illuminate the crucial role of miR-146a in the pathogenesis of human diseases associated with A. fumigatus infection.
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http://dx.doi.org/10.1007/s11046-021-00538-0DOI Listing
June 2021

An Update on the Progress of Endometrial Receptivity in Women with Polycystic Ovary Syndrome.

Reprod Sci 2021 Jun 2. Epub 2021 Jun 2.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Xi'an Jiaotong University, No.157 of Xiwu Road, Xi'an, People's Republic of China.

Polycystic ovary syndrome (PCOS) is a significant public health issue with diverse presentations, including reproductive, metabolic, and psychological disorders. Although problems with ovulation, metabolism, and hormonal imbalance can be pharmacologically improved, even the excellent quality of transferred embryos does not necessarily increase the pregnancy rate. Poor endometrial receptivity in women with PCOS perturbs endometrial decidualization and blastocyst implantation, increasing adverse pregnancy outcomes, such as miscarriage and poor embryonic development. The etiological and pathophysiological mechanisms involved in defective endometrial receptivity in women with PCOS have not been fully elucidated to date. Various contributing factors have been reported as primary causes of defective endometrial receptivity in women with PCOS, including metabolic alterations, inflammatory events, and some abnormally expressed endometrial molecular markers. However, few studies to date have investigated in depth the complex mechanisms underlying the compromised endometrial receptivity in women with PCOS. This article reviews recent reports mainly on metabolic alterations and some new endometrial molecular markers in order to collate the existing data and improve our understanding in this field. The aim was to discuss current novel insights on defective endometrial receptivity in women with PCOS in order to provide a theoretical basis for reducing adverse pregnancy outcomes and improving the live birth rate in PCOS.
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http://dx.doi.org/10.1007/s43032-021-00641-zDOI Listing
June 2021

Measurement of Exhaled Nitric Oxide in 456 Lung Cancer Patients Using a Ringdown FENO Analyzer.

Metabolites 2021 May 31;11(6). Epub 2021 May 31.

Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 100730, China.

The objective of this study was to investigate the clinical value of exhaled nitric oxide (NO) for diagnosing lung cancer patients by using a relatively large sample. An online and near-real-time ringdown exhaled NO analyzer calibrated by an electrochemical sensor at clinical was used for breath analysis. A total of 740 breath samples from 284 healthy control subjects (H) and 456 lung cancer patients (LC) were collected. The recorded data included exhaled NO, medications taken within the last half month, demographics, fasting status and smoking status. The LC had a significantly higher level of exhaled NO than the H (H: 21.0 ± 12.1 ppb vs. LC: 34.1 ± 17.2 ppb). The area under the receiver operating characteristic curve for exhaled NO predicting LC and H was 0.728 (sensitivity was 0.798; specificity was 0.55). There was no significant difference in exhaled NO level between groups divided by different types of LC, tumor node metastasis (TNM) stage, sex, smoking status, age, body mass index (BMI) or fasting status. Exhaled NO level alone is not a useful clinical tool for identifying lung cancer, but it should be considered when developing a diagnosis model of lung cancer by using breath analysis.
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http://dx.doi.org/10.3390/metabo11060352DOI Listing
May 2021

Epidemiology, Genetic Characterization, and Evolution of Carried by and : The First Epidemiological Evidence from Southern China.

Pathogens 2021 May 28;10(6). Epub 2021 May 28.

Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou 510515, China.

is a novel member of the family . A single species, , is currently described. However, there is limited information on the identification of to date, and thereby the circulation of is not fully understood. Thus, the objective of this study was to investigate the prevalence, genomic characteristics, and evolution of rat hunnivirus in southern China. A total of 404 fecal samples were subjected to detection of from urban rats ( and ) using PCR assay based on specific primers targeted to partial 3D regions, with the prevalence of 17.8% in and 15.6% in . An almost full-length rat hunnivirus sequence (RatHuV/YY12/CHN) and the genome structure were acquired in the present study. Phylogenetic analysis of the P1 coding regions suggested the RatHuV/YY12/CHN sequence was found to be within the genotype of . The negative selection was further identified based on analysis of non-synonymous to synonymous substitution rates. The present findings suggest that hunniviruses are common in urban rats. Further research is needed for increased surveillance and awareness of potential risks to human health.
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http://dx.doi.org/10.3390/pathogens10060661DOI Listing
May 2021

Sensitive and Label-free Detection of Bacteria in Osteomyelitis through Exo III-Assisted Cascade Signal Amplification.

ACS Omega 2021 May 30;6(18):12223-12228. Epub 2021 Apr 30.

Service Centre, Taizhou Hospital of Zhejiang Province, Linhai 317000, China.

Rapid and sensitive pathogenic bacterial identification and isolation from complicated clinical specimens are of great importance for the early diagnosis and prevention of osteomyelitis. Herein, we proposed a novel methicillin-resistant (MRSA) detection strategy through two specially designed streptavidin magnetic bead-based probes, including a capture probe and a report probe. In detail, the capture probe takes the responsibility to specially bind with the surface protein of MRSA and leads to the liberation of the promoter which could subsequently initiate report probe-based signal amplification. Afterward, the hybridization of the promoter probe with the report probe could then transform the protruding 3' terminus of template DNA in the report probe into a blunt end. With the assistance of Exo III, the template could be digested to liberate the promoter to form a recycle and to liberate the biprobe to induce the following rolling circle amplification (RCA)-based signal amplification. Through the integration of the Exo III-assisted recycle and RCA-based signal amplification, the proposed method exhibited a favorable detection performance.
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http://dx.doi.org/10.1021/acsomega.1c01107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154161PMC
May 2021

Prevalence and contributory factors of anxiety and depression among pregnant women in the post-pandemic era of COVID-19 in Shenzhen, China.

J Affect Disord 2021 May 18;291:243-251. Epub 2021 May 18.

Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, Guangzhou 510515, Guangdong, China. Electronic address:

Background: Pregnant women are emotionally vulnerable and have suffered great psychological impacts. Following the coronavirus disease 2019 (COVID-19) outbreak, a study was undertaken of the prevalence of, and factors contributing to, symptoms of anxiety and depression among pregnant women in Shenzhen, China.

Methods: A cross-sectional study on pregnant women was conducted from September to December 2020 in Shenzhen, using a random-recruit method. The General Anxiety Disorder (GAD-7) and Patient Health Questionnaire (PHQ-9) surveys were used to evaluate symptoms of anxiety and depression. A multivariate logistic regression model was developed to explore factors potentially associated with symptoms of anxiety and depression during pregnancy.

Results: A total of 3,434 pregnant women aged 15 to 59 years were enrolled. Symptoms of anxiety and depression were present in 9.8% and 6.9%, respectively. Logistic regression analysis using a stepwise procedure revealed that an increased risk of symptoms of anxiety and depression was associated with unmarried/divorced/widowed, unemployed, received professional psychological counseling, family dysfunction, the first trimester of pregnancy, pregnancy complications and vaginal bleeding, unplanned pregnancy, decline in household income and disputes between partners caused by the COVID-19 pandemic, consumption of alcoholic drinks by women and their partners, smoking, lack of exercise and sedentary lifestyle. Women with education from junior high school through college were less likely to experience symptoms of prenatal depression.

Conclusions: Our study revealed factors associated with psychological symptoms among pregnant women in the post-COVID-19-pandemic era. These results should help to update guidance for psychological interventions for pregnant women during the period of COVID-19.
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http://dx.doi.org/10.1016/j.jad.2021.05.014DOI Listing
May 2021

Health risk assessment of heavy metals in marine fish to the population in Zhejiang, China.

Sci Rep 2021 May 26;11(1):11079. Epub 2021 May 26.

Zhejiang Provincial Center for Disease Control and Prevention, Room No. 401, Bin-Sheng Road No. 3399, Binjiang District, Hangzhou City, 310051, China.

Environmental pollution with toxic metals can lead to the possible contamination of the marine fish. We investigated the levels of As, Cd, Cr, Hg and Pb in 652 marine fish samples (15 species) collected from coastal areas of Zhejiang, China and estimated their health risk. Mean concentrations of As, Cd, Cr, Hg and Pb were 0.783, 0.009, 0.114, 0.031, 0.043 mg/kg wet weight. The average estimated daily intakes (EDIs) for As, Cd, Cr, Hg and Pb were 1.214, 0.014, 0.177, 0.048 and 0.067 μg/kg bw/day. The risk assessment at mean exposure level showed that there was no health risk associated with these elements through consumption of marine fish. However, potential health risk may exist for high exposure consumers considering the possible contamination of As and Hg. Given that the different levels of certain elements in marine fish in China, this study provides a scientific basis for food safety assessment and suggestions for risk management.
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http://dx.doi.org/10.1038/s41598-021-90665-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155212PMC
May 2021

High-Performance Room-Temperature UV-IR Photodetector Based on the InAs Nanosheet and Its Wavelength- and Intensity-Dependent Negative Photoconductivity.

ACS Appl Mater Interfaces 2021 Jun 25;13(22):26187-26195. Epub 2021 May 25.

Key Laboratory for the Physics and Chemistry of Nanodevices and Department of Electronics, Peking University, Beijing 100871, China.

Low-dimensional narrow-band-gap III-V semiconductors have great potential in high-performance electronics, photonics, and quantum devices. However, high-performance nanoscale infrared photodetectors based on isolated two-dimensional (2D) III-V compound semiconductors are still rare. In this work, we demonstrate a new type of photodetector based on the InAs nanosheet. The photodetector has high optoelectronic response in the ultraviolet-infrared band (325-2100 nm) at room temperature. The high-performance photodetector has very high responsivity (∼1231 A/W), EQE (2.2 × 10 %), and detectivity (5.46 × 10 Jones) to 700 nm light at low operating voltage (∼0.1 V). These results indicate that 2D InAs nanosheet devices have great potential in nano-optoelectronic devices and integrated optoelectronic devices. In addition, we observe for the first time that the InAs nanosheet devices have a negative photoconductivity (NPC) that is not only affected by the wavelength but also related to the optical power intensity of the light. After analyzing experimental data, we propose that the origin of the NPC may come from electron trapping, and two competing mechanisms of optical absorption and the photogating effect in the photoelectric response process cause the dependence on the light wavelength and optical power intensity.
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http://dx.doi.org/10.1021/acsami.1c05226DOI Listing
June 2021

Pretreatment by composting increased the utilization proportion of pig manure biogas digestate and improved the seedling substrate quality.

Waste Manag 2021 Jun 21;129:47-53. Epub 2021 May 21.

College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.

Anaerobic digestion of organic residues has the potential to significantly contribute to a shift from fossil to renewable energy, while the remaining biogas digestate need to be treated or used for a second time. In this study, the pig manure biogas digestate (PMBD) was evaluated as a potential part of seedling substrates and composting was considered a pretreating method to improve its characters. Composting was carried out firstly in a forced aeration composting system (100 L), in which perlite and sawdust were used as additives in different proportions separately or together. Based on the comparison of the physicochemical characters of different seedling substrates formulas mixed with PMBD or pig manure biogas digestate pretreated by composting (CPMBD), selected seedling substrates were analyzed by bioassay experiment. The results showed that pretreatment by composting and the additives (perlite and sawdust) used in composting decreased the pH value of PMBD and make it suitable for seedling substrates, especially composted with perlite and sawdust. Both PMBD and CPMBD with low proportion in the substrates improved plant growth of lettuce and tomato, while CPMBD was better than PMBD. However, when the proportions of PMBD was increased more than 20% and CPMBD was increased more than 40%, plant growth inhibition was observed. Tomato was more sensitive than lettuce to the physicochemical characters of the substrate. In summary, PMBD pretreated by composting not only increases the uses of digestate, but also enhances plant growth and hence yield.
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http://dx.doi.org/10.1016/j.wasman.2021.05.010DOI Listing
June 2021

Characteristics of mental health implications and plasma metabolomics in patients recently recovered from COVID-19.

Transl Psychiatry 2021 05 21;11(1):307. Epub 2021 May 21.

Department of Respiratory and Critical Care Medicine, NHC Key Laboratory of Pulmonary Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, Hubei, China.

This study aimed to explore the associations between cerebral white matter (WM) alterations, mental health status, and metabolism in recovered COVID-19 patients. We included 28 recovered COVID-19 patients and 27 healthy controls between April 2020 and June 2020. Demographic data, the mental health scores, diffusion-tensor imaging (DTI) data, and plasma metabolomics were collected and compared between the two groups. Tract-based spatial statistics and graph theory approaches were used for DTI data analysis. Untargeted metabolomics analysis of the plasma was performed. Correlation analyses were performed between these characteristics. Recovered COVID-19 patients showed decreased fractional anisotropy, increased mean diffusivity and radial diffusivity values in widespread brain regions, and significantly lower global efficiency, longer shortest path length, and less nodal local efficiency in superior occipital gyrus (all, P < 0.05, Bonferroni corrected). Our results also demonstrated significantly different plasma metabolic profiling in recovered COVID-19 patients even at 3 months after their hospital discharge, which was mainly related to purine pathways, amino acids, lipids, and amine metabolism. Certain regions with cerebral WM alterations in the recovered patients showed significant correlations with different metabolites and the mental health scores. We observed multiple alterations in both WM integrity and plasma metabolomics that may explain the deteriorated mental health of recovered COVID-19 patients. These findings may provide potential biomarkers for the mental health evaluation for the recovered COVID-19 patients and potential targets for novel therapeutics.
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http://dx.doi.org/10.1038/s41398-021-01426-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138845PMC
May 2021

Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells.

Int J Nanomedicine 2021 10;16:3201-3216. Epub 2021 May 10.

Spine Center, Department of Orthopaedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, People's Republic of China.

Purpose: Polyetheretherketone (PEEK) exhibits high mechanical strengths and outstanding biocompatibility but biological inertness that does not excite the cell responses and stimulate bone formation. The objective of this study was to construct submicro-nano structures on PEEK by femtosecond laser (FSL) for exciting the responses of MC3T3-E1 cells and gingival epithelial (GE) cells, which induce regeneration of bone/gingival tissues for long-term stability of dental implants.

Materials And Methods: In this study, submicro-nano structures were created on PEEK surface by FSL with power of 80 mW (80FPK) and 160 mW (160FPK).

Results: Compared with PEEK, both 80FPK and 160FPK with submicro-nano structures exhibited elevated surface performances (hydrophilicity, surface energy, roughness and protein absorption). Furthermore, in comparison with 80FPK, 160FPK further enhanced the surface performances. In addition, compared with PEEK, both 80FPK and 160FPK significantly excited not only the responses (adhesion, proliferation, alkaline phosphatase [ALP] activity and osteogenic gene expression) of MC3T3-E1 cells but also responses (adhesion as well as proliferation) of GE cells of human in vitro. Moreover, in comparison with 80FPK, 160FPK further enhanced the responses of MC3T3-E1 cells/GE cells.

Conclusion: FSL created submicro-nano structures on PEEK with elevated surface performances, which played crucial roles in exciting the responses of MC3T3-E1 cells/GE cells. Consequently, 160FPK with elevated surface performances and outstanding cytocompatibility would have enormous potential as an implant for dental replacement.
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http://dx.doi.org/10.2147/IJN.S303411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121686PMC
May 2021

Novel intergenic fusion variant in a patient with gastric cancer: A case report.

World J Clin Cases 2021 May;9(14):3350-3355

Department of Oncology, The Seventh Affiliated Hospital of Yangzhou University, Jingjiang People's Hospital, Jingjiang 214500, Jiangsu Province, China.

Background: fusion is a key driver mutation, but it is rare in gastric cancer (GC). Several (hepatocyte growth factor receptor) inhibitors have been approved for the treatment of -positive patients, but the tumor response is heterogeneous. With the development of next-generation sequencing, diverse fusion partner genes have been identified. We herein report a fusion variant involving - in GC.

Case Summary: After thoracoscopic inferior lobectomy plus lymph node dissection under general anesthesia, a "tumor within a tumor" was found in the lung tumor tissue of a 64-year-old non-smoking male patient. Combining the medical history and the results of enzyme labeling, the focal area was considered to be GC. To seek potential therapeutic regimens, an intergenic region between and fusion was identified. This fusion contains a kinase domain and coil-coiled domains encoded by exons 1-25, which might drive the oncogenesis.

Conclusion: Our finding could extend the spectrum and genomic landscape of fusions in GC and favor the development of personalized therapy.
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http://dx.doi.org/10.12998/wjcc.v9.i14.3350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107910PMC
May 2021

miR-942-5p Inhibits Proliferation, Metastasis, and Epithelial-Mesenchymal Transition in Colorectal Cancer by Targeting CCBE1.

Biomed Res Int 2021 28;2021:9951405. Epub 2021 Apr 28.

Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.

Although colorectal cancer (CRC) is common, there is a paucity of information regarding its molecular pathogenesis. Studies have shown that miRNAs play pivotal roles in the development and progression of CRC. There is a need to further investigate the biological functions of miRNAs in CRC. In particular, it has been reported that miR-942-5p exhibits tumor-suppressive properties. Thus, we analyzed the functional significance of miR-942-5p in CRC and the underlying molecular mechanisms. We found that miR-942-5p was downregulated in CRC tissues and cells. Cell Counting Kit-8, EdU, and colony formation assays revealed that the overexpression of miR-942-5p by mimics inhibited the proliferation of CRC cells. Use of the miR-942-5p inhibitor effectively enhanced the proliferative potential of CRC cells. Further, xenograft experiments confirmed these results. Increased expression of miR-942-5p suppressed the invasion, migration, and epithelial-mesenchymal transition of CRC cell lines, while decreased miR-942-5p expression had the opposite effect. CCBE1, a secretory molecule for lymphangiogenesis, was established as a downstream target of miR-942-5p, and its expression was inversely correlated with the expression of miR-942-5p in CRC cells. Additionally, cotransfection of the miR-942-5p inhibitor with si-CCBE1 into CRC cells reversed the effects induced by miR-942-5p overexpression. In conclusion, we confirmed that miR-942-5p exerts oncogenic actions in CRC by targeting CCBE1 and identified miR-942-5p as a potential clinical biomarker for CRC diagnosis and therapy.
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http://dx.doi.org/10.1155/2021/9951405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102100PMC
May 2021

Age-stratified and gender-specific reference intervals of six tumor markers panel of lung cancer: A geographic-based multicenter study in China.

J Clin Lab Anal 2021 Jun 12;35(6):e23816. Epub 2021 May 12.

Division of in vitro Diagnostics, Shenzhen Mindray Bio-Medical Electronics Corporation, Shenzhen, China.

Background: Serum biomarkers have been widely adopted in clinical practice for assisting lung cancer diagnoses, therapeutic monitoring, and prognostication. The function of a well-performing tumor biomarker depends on a reliable reference interval (RI) with consideration of the study subjects' age, gender, and geographical location. This study aimed to establish a RI for each of 6 lung cancer biomarkers for use in the whole country of China on Mindray platform.

Methods: The levels of serum 6 lung cancer biomarkers-namely progastrin-releasing peptide (ProGRP), neuron-specific enolase (NSE), squamous cell carcinoma antigen (SCC), carcinoembryonic antigen (CEA), cytokeratin-19 fragment (CYFRA21-1), and human epididymis protein 4 (HE4)-were measured utilizing the chemiluminescence immunoassay on the Mindray CL-6000i platform following the laboratory standard operating procedures in apparently healthy Chinese individuals on large cohort, multicenter, and geographical consideration bases. The CLSI EP28-A3C guideline was followed for the enrollment of study subjects.

Results: The age-stratified, gender-specific RIs for ProGRP, NSE, SCC, CEA, CYFRA21-1, and HE4 lung cancer biomarkers in the Chinese population have been established as described in the results and discussion in this work. In addition, various levels of the six lung cancer biomarkers among nine geographical locations in China have been observed.

Conclusions: The sample volume of study cohort, age, and geographical location should be considered upon establishing a reliable biomarker RI. A RI for each of six lung cancer biomarkers has been established. The results from this study would be helpful for clinical laboratories in interpreting the analytical results and for clinicians in patient management.
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http://dx.doi.org/10.1002/jcla.23816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183943PMC
June 2021

Shifts in enzymatic activities and microbial community structures in the bioenhanced treatment of ship domestic sewage under microaerobic conditions.

Environ Sci Pollut Res Int 2021 May 12. Epub 2021 May 12.

Marine Engineering College, Dalian Maritime University, Dalian, 116026, People's Republic of China.

A bioenhancement strategy for improving the anaerobic degradation efficiency of ship domestic sewage under microaerobic conditions was proposed in this study. Strains Stenotrophomonas sp. MSPP05 and Prevotella sp. MSPP07 with high organic-degrading efficiency and extracellular hydrolase yield were used for the bioenhancement of activated sludge. In batch experiments, the removal rates of chemical oxygen demand and total nitrogen reached 94.5% and 66.9% after 72 h of degradation. The activities of dehydrogenase, extracellular amylase, and protease in the treatment group were 1.2, 1.4, and 2.0 times higher than those in the control group. Microbial community analysis showed that exogenous enhanced strains competed with original microorganisms and became dominant. One-stage continuous stirred tank reactor with bioenhanced activated sludge ran steadily for 90 days with average effluent COD and TN concentrations of 87.5 and 14.6 mg/L. The feasibility of improving organic-degrading efficiency through bioenhancement by using exogenous hydrolase-producing strains was confirmed under microaerobic conditions. This work provided a theoretical basis for improving treatment effects and developing a new technique for ship domestic sewage treatment.
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http://dx.doi.org/10.1007/s11356-021-14232-7DOI Listing
May 2021

Possibility of inducing tumor cell senescence during therapy.

Oncol Lett 2021 Jul 27;22(1):496. Epub 2021 Apr 27.

School of Pharmaceutical Sciences and Yunnan Provincial Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, Yunnan 650500, P.R. China.

The treatment options for cancer include surgery, radiotherapy and chemotherapy. However, the traditional approach of high-dose chemotherapy brings tremendous toxic side effects to patients, as well as potentially causing drug resistance. Drug resistance affects cell proliferation, cell senescence and apoptosis. Cellular senescence refers to the process in which cells change from an active proliferative status to a growth-arrested status. There are multiple factors that regulate this process and cellular senescence is activated by various pathways. Senescent cells present specific characteristics, such as an increased cell volume, flattened cell body morphology, ceased cell division and the expression of β-galactosidase. Tumor senescence can be categorized into replicative senescence and premature senescence. Cellular senescence may inhibit the occurrence and development of tumors, serving as an innovative strategy for the treatment of cancer. The present review mainly focuses on senescent biomarkers, methods for the induction of cellular senescence and its possible application in the treatment of cancer.
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http://dx.doi.org/10.3892/ol.2021.12757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108274PMC
July 2021

Taishania pollutisoli gen. nov., sp. nov., Isolated from Tetrabromobisphenol A-Contaminated Soil.

Curr Microbiol 2021 May 11. Epub 2021 May 11.

Key Lab of Microbiological Engineering of Agricultural Environment, Department of Microbiology, College of Life Sciences, Ministry of Agriculture, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China.

Strain CZZ-1 was isolated from long-term TBBPA-contaminated soil Zaozhuang city, Shandong province, People's Republic of China. CZZ-1 was pink-pigmented, Gram-stain-negative, rod-shaped, non-motile and aerobic. The 16S rRNA gene analysis indicated that strain CZZ-1 shows high similarities to Fluviicola taffensis DSM 16823 (92.6%) and Fluviicola hefeinensis KACC 16597 (92.5%) and less than 91% sequence similarities to other genus or species in the family Crocinitomicaceae. It was able to grow at 10-37 °C, with 0-6% (w/v) NaCl. It could hydrolyze gelatin, but could not reduce nitrates to nitrites. The predominant fatty acids of strain CZZ-1 were iso-C (51.3%), C 2-OH (11.0%), iso-C 3-OH (8.0%), C (7.0%), iso-C G (6.8%) and Summed Feature 3 (C ω7c and/or C ω6c, 4.4%). The polar lipid profile was composed of five unidentified lipids, two unidentified phospholipids, one phosphatidylethanolamine, one unidentified aminolipid and one unidentified glycolipid. The predominant respiratory quinone was MK-6. The genomic DNA G+C content of strain CZZ-1 was 41.5 mol%. Based on data from phenotypic, chemotaxonomic and genotypic analysis in this study, strain CZZ-1 represents a novel species in a new genus in the family Crocinitomicaceae, for which the name Taishania pollutisoli gen. nov., sp. nov. is proposed. The type strain is CZZ-1 (= KCTC 52343 = GDMCC 1.2270).
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http://dx.doi.org/10.1007/s00284-021-02522-9DOI Listing
May 2021

Regulating Twisted Skeleton to Construct Organ-Specific Perylene for Intensive Cancer Chemotherapy.

Angew Chem Int Ed Engl 2021 May 10. Epub 2021 May 10.

CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology (NCNST), Beijing, 100190, China.

The systemic use of pharmaceutical drugs for cancer patients is a compromise between desirable therapy and side effects because of the intrinsic shortage of organ-specific pharmaceutical drug. Design and construction of pharmaceutical drug to achieve the organ-specific delivery is thus desperately desirable. We herein regulate perylene skeleton to effect organ-specificity and present an example of lung-specific distribution on the basis of bay-twisted PDIC-NC. We further demonstrate that PDIC-NC can target into mitochondria to act as cellular respiration inhibitor, inducing insufficient production of adenosine triphosphate, promoting endogenous H O and OH burst, elevating calcium overload, efficiently triggering the synergistic apoptosis, autophagy and endoplasmic reticulum stress of lung cancer cells. The antitumor performance of PDIC-NC is verified on in vivo xenografted, metastasis and orthotopic lung cancer, presenting overwhelming evidences for potentially clinical application. This study contributes a proof-of-concept demonstration of twisted perylene to well attain lung-specific distribution, and meanwhile achieves intensive lung cancer chemotherapy.
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http://dx.doi.org/10.1002/anie.202105607DOI Listing
May 2021

Study on structural characterization, physicochemical properties and digestive properties of euryale ferox resistant starch.

Food Chem 2021 Oct 22;359:129924. Epub 2021 Apr 22.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.

At present, the raw materials for industrialized RS3 products are relatively simple and its purity is low. In addition, the correlation between structure and digestion characteristics of RS3 are rarely studied. In this study, euryale ferox, a kind of annual aquatic herb crop with high content of starch was used as a raw material to prepare RS3 by different methods, including autoclaving, enzymolysis-autoclaving and dual enzymolysis, respectively. The results showed that there were significant differences in the structure and physicochemical properties of the different euryale ferox resistant-enhanced and purified resistant starches (p < 0.05). Purified euryale ferox resistant starches belonged to B + V type crystal and had high thermal stability. After digestion, the structure and thermal properties of euryale ferox resistant-enhanced starches changed a lot. The digestion rate and estimated glycemic index (eGI) of the three kinds of purified euryale ferox RS3 were lower than 20% and 50%, respectively.
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http://dx.doi.org/10.1016/j.foodchem.2021.129924DOI Listing
October 2021

Cryo-EM structure of ABCG5/G8 in complex with modulating antibodies.

Commun Biol 2021 May 5;4(1):526. Epub 2021 May 5.

Department of Therapeutics Discovery, Amgen Research, Amgen Inc., South San Francisco, CA, USA.

The heterodimer of ATP-binding cassette transporter ABCG5 and ABCG8 mediates the excretion of sterols from liver and intestine, playing a critical role in cholesterol homeostasis. Here, we present the cryo-EM structure of ABCG5/G8 in complex with the Fab fragments from two monoclonal antibodies at 3.3Å resolution. The high-resolution structure reveals a unique dimer interface between the nucleotide-binding domains (NBD) of opposing transporters, consisting of an ordered network of salt bridges between the conserved NPXDFXXD motif and serving as a pivot point that may be important for the transport cycle. While mAb 11F4 increases the ATPase activity potentially by stabilization of the NBD dimer formation, mAb 2E10 inhibits ATP hydrolysis, likely by restricting the relative movement between the RecA and helical domain of ABCG8 NBD. Our study not only provides insights into the structural elements important for the transport cycle but also reveals novel epitopes for potential therapeutic interventions.
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http://dx.doi.org/10.1038/s42003-021-02039-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100176PMC
May 2021

On the redox property of AgAu clusters: One-way conversion from anionic [AuAgL] to charge neutral [AuAgL].

J Chem Phys 2021 Apr;154(16):164308

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

The redox property of metal nanoclusters plays a pivotal role and is of particular interest in catalysis and other applications, such as aerobic oxidation, hydrogenation, and singlet oxygen generation, over intact nanoclusters. In this study, we report a one-way conversion process of the anionic [AgAuL] nanocluster into a charge neutral nanocluster of [AgAuL] via oxidation in a solution phase using HO as the oxidant. Three-electron loss of [AgAuL] occurred during the oxidation process, which was confirmed by electron paramagnetic resonance and electrospray ionization mass spectrometry methods. The one-way conversion from [AgAuL] to [AgAuL] nanoclusters is in situ monitored by UV-visible spectroscopy. A nanocluster charge effect is manifested in the UV-visible spectra of nanoclusters; an ∼10 nm redshift is observed compared with the optical absorption spectrum of [AgAuL].
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http://dx.doi.org/10.1063/5.0048931DOI Listing
April 2021