Publications by authors named "Qing Cai"

397 Publications

The neural mechanism of spatial-positional association in working memory: A fMRI study.

Brain Cogn 2021 May 26;152:105756. Epub 2021 May 26.

Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, China; School of Psychology, Center for Studies of Psychological Application, and Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, China. Electronic address:

In the last decade, research has reported that items at the beginning of a memorized sequence are responded to faster with the left hand, whereas items at the end are responded to faster with the right hand. This Spatial-Positional Associations of Response Codes effect has been extensively studied using behavioral methods. However, the neural networks underlying it remain unclear. We found using functional magnetic resonance imaging (fMRI) that the dorsal attention network was involved in spatial-positional associations, in particular a region of the right superior frontal cortex / pre-supplementary motor area (pre-SMA), within which neural activity correlated with behavioral measures of the strength of spatial-positional associations. Psychophysiological interaction (PPI) analysis revealed functional connectivity between this area and other regions of the dorsal attentional network including the SMA, and with the hippocampal-retrosplenial network. In contrast, explicit processing of serial order independent of spatial-positional associations was related to activity in the inferior parietal cortex. Our results provide new insight into positional coding theories of working memory, including the mental whiteboard hypothesis. They suggest that the behavioral effects of positional coding (congruency between hand and ordinal position within the list) are mediated through spatial and motor control maps in the dorsal attentional system.
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http://dx.doi.org/10.1016/j.bandc.2021.105756DOI Listing
May 2021

Dissociable plasticity of visual-motor system in functional specialization and flexibility in expert table tennis players.

Brain Struct Funct 2021 May 26. Epub 2021 May 26.

Key Laboratory of Adolescent Health Assessment and Exercise Intervention of Ministry of Education, College of Physical Education and Health, East China Normal University, Shanghai, 200062, China.

Specialization and flexibility are two basic attributes of functional brain organization, enabling efficient cognition and behavior. However, it is largely unknown what plastic changes in specialization and flexibility in visual-motor areas occur in support of extraordinary motor skills in expert athletes and how the selective adaptability of the visual-motor system affects general perceptual or cognitive domains. Here, we used a dynamic network framework to investigate intrinsic functional specialization and flexibility of visual-motor system in expert table tennis players (TTP). Our results showed that sensorimotor areas increased intrinsic functional flexibility, whereas visual areas increased intrinsic functional specialization in expert TTP compared to nonathletes. Moreover, the flexibility of the left putamen was positively correlated with skill level, and that of the left lingual gyrus was positively correlated with behavioral accuracy of a sport-unrelated attention task. This study has uncovered dissociable plasticity of the visual-motor system and their predictions of individual differences in skill level and general attention processing. Furthermore, our time-resolved analytic approach is applicable across other professional athletes for understanding their brain plasticity and superior behavior.
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http://dx.doi.org/10.1007/s00429-021-02304-wDOI Listing
May 2021

Mobilization and geochemistry of nutrients in sediment evaluated by diffusive gradients in thin films: Significance for lake management.

J Environ Manage 2021 Aug 18;292:112770. Epub 2021 May 18.

State Environmental Protection Key Laboratory for Lake Pollution Control, Institute of Lake Environment, Chinese Research Academy of Environmental Sciences (CRAES), Beijing, 100012, China; College of Water Science, Beijing Normal University, Beijing, 100875, China.

Investigation of in-situ mobilization of both nitrogen (N) and phosphate (PO) in sediment is important for lake management strategy. In this paper, diffusion gradients in thin films (DGT) and DGT induced flux in sediments (DIFS) model are newly designed for in-situ measurement of iron (Fe), PO, nitrate (NO-N) and ammonium (NH-N), and nutrients' mobility in sediment in Lake Nanhu (China). According to DGT profiles together with physicochemical properties in sediment, (I) PO is released from (i) Fe-bound P plus loosely sorbed P in anoxic sediment and (ii) the loosely sorbed P in oxic sediment; (II) anoxic sediment inhibits nitrification and NO-N release, but it favors denitrification and dissimilatory nitrate reduction to ammonium (DNRA), leading to NH-N release; (III) Eh and organic matter are two key influence factors on mobility of PO, NO-N and NH-N. According to DIFS calculation, the dynamics of desorption and diffusion at two sites belong to (i) slow rate of resupply and (ii) fast resupply cases, respectively. Internal loadings are estimated to be 92.74 (PO), 268.1 (NH-N) and -2466 kg a (NO-N), which reflects sediment mainly acts as a source for PO and NH-N, and a sink for NO-N in water. Based on sediment P release risk index (SPRRI), P release risks in lake sediments are estimated, ranging from light to relative high level. DGT and SPRRI aid choice of restoration methods for sediment, including sediment dredging, phytoremediation and in-situ inactivation.
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http://dx.doi.org/10.1016/j.jenvman.2021.112770DOI Listing
August 2021

Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout.

Analyst 2021 May;146(10):3299-3304

Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, China.

The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection. In this work, a thin film fabricated from a hyaluronic acid (HA)-polyethyleneimine (PEI) hydrogel has been covered on a mixed cellulose microporous membrane (MCEM) to form a HA-PEI-MCEM firstly and it was then applied in a filtration system. The permeability of the filter membrane greatly affects the amount of water passing through within a certain time and the water can be collected and quantitatively measured with a simple electronic balance easily. The low permeability of the HA-PEI-MCEM allows a small amount of water to be drained. But after the addition of HAase, which can hydrolyze HA in the hydrogel, the permeability of the membrane increased. Therefore, the amount of water passing through the HA-PEI-MCEM composite membrane increased accordingly. The composite of the membrane, and the reaction conditions after the addition of HAase were optimized. Under the optimized conditions, the amount of water collected within 5 min showed a linear relationship with the HAase concentration in a range of 1.0-36 U mL-1 with a limit of detection of 0.35 U mL-1.The proposed method has been applied to detect HAase in vitreous samples with satisfactory results.
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http://dx.doi.org/10.1039/d1an00257kDOI Listing
May 2021

Effects of Hyperoxia on Mitochondrial Homeostasis: Are Mitochondria the Hub for Bronchopulmonary Dysplasia?

Front Cell Dev Biol 2021 30;9:642717. Epub 2021 Apr 30.

Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang City, China.

Mitochondria are involved in energy metabolism and redox reactions in the cell. Emerging data indicate that mitochondria play an essential role in physiological and pathological processes of neonatal lung development. Mitochondrial damage due to exposure to high concentrations of oxygen is an indeed important factor for simplification of lung structure and development of bronchopulmonary dysplasia (BPD), as reported in humans and rodent models. Here, we comprehensively review research that have determined the effects of oxygen environment on alveolar development and morphology, summarize changes in mitochondria under high oxygen concentrations, and discuss several mitochondrial mechanisms that may affect cell plasticity and their effects on BPD. Thus, the pathophysiological effects of mitochondria may provide insights into targeted mitochondrial and BPD therapy.
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http://dx.doi.org/10.3389/fcell.2021.642717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120003PMC
April 2021

Cognitive control and emotional response in attention-deficit/ hyperactivity disorder comorbidity with disruptive, impulse-control, and conduct disorders.

BMC Psychiatry 2021 05 4;21(1):232. Epub 2021 May 4.

Clinical Research Center, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030, China.

Background: This study investigated cognitive and emotional functioning in children and adolescents with attention-deficit/hyperactivity disorder (ADHD) and disruptive, impulse-control, and conduct disorders (DICCD).

Methods: Thirty patients with ADHD, 26 with DICCD, 22 with ADHD+DICCD were recruited from the outpatient department of Shanghai Changning Mental Health Center, plus 20 healthy controls (HC). Differences between the groups in cognitive and emotional functioning were examined using Golden's Stroop and Emotional Stroop tests. For Emotional Stroop Mean reaction time (RT) of positive word (POS) and negative word (NEG) with color congruence (C) or incongruence (I) were recorded as POS-C, POS-I, NEG-C and NEG-I, respectively.

Results: For Golden's interference scores (IGs), both errors and RTs in the ADHD group were higher than in the other groups. Longer mean RTs of POS-C, POS-I, NEG-C and neural word (NEU) of the ADHD group, and NEG-I of ADHD+DICCD and DICCD groups were observed compared to HC. After 12 weeks of methylphenidate treatment, differences between ADHD subgroups and HC on Golden's Stroop RT disappeared, but differences in Golden's Stroop errors and Emotional Stroop mean RTs remained. The ADHD+DICCD group showed longer mean RTs in NEG-C, NEG-I and NEU of the Emotional Stroop test than the ADHD group.

Conclusions: Our study shows that regardless of emotional responding, deficit in cognitive control is the core symptom of ADHD. However, emotionally biased stimuli may cause response inhibitory dysfunction among DICCD with callous-unemotional traits, and the comorbidity of ADHD and DICCD tends to account for the negative emotional response characteristic of DICCD. These deficits may be eliminated by medication treatment in ADHD, but not the ADHD with comorbid DICCD. Our results support the notion that ADHD with comorbid DICCD is more closely related to DICCD than to ADHD.
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http://dx.doi.org/10.1186/s12888-021-03221-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094501PMC
May 2021

Composites made of polyorganophosphazene and carbon nanotube up-regulating osteogenic activity of BMSCs under electrical stimulation.

Colloids Surf B Biointerfaces 2021 Apr 24;204:111785. Epub 2021 Apr 24.

State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, 100029, China.

Bone is an electrically responsive tissue, so electroactive materials that can deliver electrical cues to bone are helpful for enhancing regeneration under electrical stimulation (ES), and conductive materials are crucial in ES transmission to determine osteogenesis. Compared with polyesters, biodegradable polyorganophosphazenes (POPPs) show superiority in the field of bone tissue engineering thanks to their rich phosphorus/nitrogen contents, suggesting that the combination of POPPs-based conductive substrates with ES may achieve synergistic enhancements on osteogenesis. Herein, conductive composite films were fabricated by blending poly[(alanine ethyl ester)-(glycine ethyl ester)]phosphazene (PAGP) with carbon nanotubes (CNTs). After surface modification with polydopamine (PDA), bone marrow mesenchymal stromal cells (BMSCs) were cultured on the films under ES, using the cells cultured on conductive films composed of poly(L-lactide) (PLLA) and CNTs as controls. The BMSCs on PAGP/CNT films demonstrated significantly faster proliferation rates and stronger osteogenic differentiation potentials than those on PLLA/CNT films, while cell attachments on the two PDA-coated substrates were similar. Under appropriate ES, further increases in the expressions of osteogenic markers as alkaline phosphatase, collagen I and calcium deposition were identified in comparison with the cases without ES. The contributions of the osteocompatible POPPs, the substrate conductivity and the ES treatment to enhanced osteogenesis suggested new strategies for the design of bone repair materials.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111785DOI Listing
April 2021

Progress on AlGaN-based solar-blind ultraviolet photodetectors and focal plane arrays.

Light Sci Appl 2021 Apr 30;10(1):94. Epub 2021 Apr 30.

Key Laboratory of Advanced Photonic and Electronic Materials, School of Electronic Science and Engineering, Nanjing University, Nanjing, 210093, China.

Solar-blind ultraviolet (UV) photodetectors (PDs) have attracted tremendous attention in the environmental, industrial, military, and biological fields. As a representative III-nitride material, AlGaN alloys have broad development prospects in the field of solar-blind detection due to their superior properties, such as tunable wide bandgaps for intrinsic UV detection. In recent decades, a variety of AlGaN-based PDs have been developed to achieve high-precision solar-blind UV detection. As integrated optoelectronic technology advances, AlGaN-based focal plane arrays (FPAs) are manufactured and exhibit outstanding solar-blind imaging capability. Considering the rapid development of AlGaN detection techniques, this paper comprehensively reviews the progress on AlGaN-based solar-blind UV PDs and FPAs. First, the basic physical properties of AlGaN are presented. The epitaxy and p-type doping problems of AlGaN alloys are then discussed. Diverse PDs, including photoconductors and Schottky, metal-semiconductor-metal (MSM), p-i-n, and avalanche photodiodes (APDs), are demonstrated, and the physical mechanisms are analyzed to improve device performance. Additionally, this paper summarizes imaging technologies used with AlGaN FPAs in recent years. Benefiting from the development of AlGaN materials and optoelectronic devices, solar-blind UV detection technology is greeted with significant revolutions. Summarizing recent advances in the processing and properties of AlGaN-based solar-blind UV PDs and FPAs as well as AlGaN growth and doping techniques.
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http://dx.doi.org/10.1038/s41377-021-00527-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087770PMC
April 2021

Polydatin alleviates traumatic brain injury: Role of inhibiting ferroptosis.

Biochem Biophys Res Commun 2021 Jun 8;556:149-155. Epub 2021 Apr 8.

Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710038, China. Electronic address:

Secondary injury is the main cause of high mortality and poor prognosis of TBI, which has recently been suggested to be related to ferroptosis. Polydatin, a monocrystalline compound extracted from the rhizome of Polygonum, has been shown to exert potential neuroprotective effects. However, its role and mechanism in the secondary injury of TBI has not been elucidated. In this study, the inhibition of Polydatin on ferroptosis was observed both in the hemoglobin treated Neuro2A cells in vitro and in TBI mouse model in vivo, characterized by reversion of accumulation or deposition of free Fe, increased content of MDA, decreased activity of key REDOX enzyme GPx4, cell death and tissues loss. Although Polydatin corrected the increased mRNA levels of ferroptosis signaling molecules GPX4, SLC7A11, PTGS2, and ATP5G3 after TBI, TBI and Polydatin treatment had no significant effect on their protein expression. Notably, Polydatin could completely reverse the decrease of GPx4 activity after TBI in vivo and in vitro, and the effect was stronger than that of the classical ferroptosis inhibitor FER-1 in vitro. Further, Polydatin has been shown to reduce the severity of acute neurological impairment and significantly improve subacute motor dysfunction in TBI mice. Our findings provided translational insight into neuroprotection with Polydatin in TBI by inhibiting ferroptosis mainly depending on the maintenance of GPx4 activity.
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http://dx.doi.org/10.1016/j.bbrc.2021.03.108DOI Listing
June 2021

A fungal sirtuin modulates development and virulence in the insect pathogen, Beauveria bassiana.

Environ Microbiol 2021 Apr 5. Epub 2021 Apr 5.

Department of Microbiology and Cell Science, University of Florida, Bldg. 981, Museum Rd, Gainesville, FL, 32611, USA.

Chromatin transitions are mediated in part by acetylation/deacetylation post-translational modifications of histones. Histone deacetylases, e.g. sirtuins (Sir-proteins), repress transcription via promotion of heterochromatin formation. Here, we characterize the Sir2 class III histone deacetylase (BbSir2) in the environmentally and economically important fungal insect pathogen, Beauveria bassiana. BbSir2 is shown to contribute to the deacetylation of lysine residues on H3 and H4 histones. Targeted gene knockout of BbSir2 resulted in impaired asexual development, reduced abilities to utilize various carbon/nitrogen sources, reduced tolerance to oxidative, heat, and UV stress, and attenuated virulence. ΔBbSir2 cells showed disrupted cell cycle development and abnormal hyphal septation patterns. Proteomic protein acetylation analyses of wild type and ΔBbSir2 cells, revealed the differential abundance of 462 proteins, and altered (hyper- or hypo-) acetylation of 436 lysine residues on 350 proteins. Bioinformatic analyses revealed enrichment in pathways involved in carbon/nitrogen metabolism, cell cycle control and cell rescue, defense, and mitochondrial functioning. Critical targets involved in virulence included LysM effector proteins and a benzoquinone oxidoreductase implicated in detoxification of cuticular compounds. These data indicate broad effects of BbSir2 on fungal development and stress response, with identification of discrete targets that can account for the observed (decreased) virulence phenotype. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1462-2920.15497DOI Listing
April 2021

Synergistic effects of D-arginine, D-methionine and D-histidine against biofilms.

Biofouling 2021 02 7;37(2):222-234. Epub 2021 Mar 7.

Department of Dental Implantology, Hospital of Stomatology, Jilin University, Changchun, China.

biofilms are implicated in the pathology of peri-implantitis and periodontitis. In this study, D-arginine (R), D-methionine (M), D-histidine (H), and a mixture of these D-amino acids (D-AAs) were investigated as an effective therapeutic strategy against biofilms. The bacterial growth activity and minimum inhibitory concentrations were determined for each D-AA, along with the effects of the D-AAs mixture on biofilm development, morphology, structure, extracellular polysaccharides (EPS), cytotoxicity towards commensals, and bacterial structure. The D-AA mixture delayed the proliferation of , changed its membrane structure, and decreased biofilm thickness and integrity, as compared with individual D-AAs. The EPS content increased with the concentration of D-AAs. The present study shows that a 4 mM RMH, triple D-AA mixture, enhanced deleterious effects on biofilms without any cytotoxicity compared with individual D-AAs, thus providing a new strategy for the treatment of peri-implantitis and periodontitis.
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http://dx.doi.org/10.1080/08927014.2021.1893309DOI Listing
February 2021

Bilingualism and domain-general cognitive functions from a neural perspective: A systematic review.

Neurosci Biobehav Rev 2021 Jun 22;125:264-295. Epub 2021 Feb 22.

Key Laboratory of Brain Functional Genomics (MOE & STCSM), Shanghai Changning-ECNU Mental Health Center, Institute of Cognitive Neuroscience, School of Psychology and Cognitive Science, East China Normal University, China; Institute of Brain and Education Innovation, East China Normal University, China; NYU-ECNU Institute of Brain and Cognitive Science, New York University Shanghai, China. Electronic address:

A large body of research has indicated that bilingualism - through continual practice in language control - may impact cognitive functions, as well as relevant aspects of brain function and structure. The present review aimed to bring together findings on the relationship between bilingualism and domain-general cognitive functions from a neural perspective. The final sample included 210 studies, covering findings regarding neural responses to bilingual language control and/or domain-general cognitive tasks, as well as findings regarding effects of bilingualism on non-task-related brain function and brain structure. The evidence indicates that a) bilingual language control likely entails neural mechanisms responsible for domain-general cognitive functions; b) bilingual experiences impact neural responses to domain-general cognitive functions; and c) bilingual experiences impact non-task-related brain function (both resting-state and metabolic function) as well as aspects of brain structure (both macrostructure and microstructure), each of which may in turn impact mental processes, including domain-general cognitive functions. Such functional and structural neuroplasticity associated with bilingualism may contribute to both cognitive and neural reserves, producing benefits across the lifespan.
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http://dx.doi.org/10.1016/j.neubiorev.2021.02.029DOI Listing
June 2021

Injectable GelMA Cryogel Microspheres for Modularized Cell Delivery and Potential Vascularized Bone Regeneration.

Small 2021 Mar 23;17(11):e2006596. Epub 2021 Feb 23.

Department of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing, 100871, China.

Cell therapeutics hold tremendous regenerative potential and the therapeutic effect depends on the effective delivery of cells. However, current cell delivery carriers with unsuitable cytocompatibility and topological structure demonstrate poor cell viability during injection. Therefore, porous shape-memory cryogel microspheres (CMS) are prepared from methacrylated gelatin (GelMA) by combining an emulsion technique with gradient-cooling cryogelation. Pore sizes of the CMS are adjusted via the gradient-cooling procedure, with the optimized pore size (15.5 ± 6.0 µm) being achieved on the 30-min gradient-cooled variant (CMS-30). Unlike hydrogel microspheres (HMS), CMS promotes human bone marrow stromal cell (hBMSC) and human umbilical vein endothelial cell (HUVEC) adhesion, proliferated with high levels of stemness for 7 d, and protects cells during the injection process using a 26G syringe needle. Moreover, CMS-30 enhances the osteogenic differentiation of hBMSCs in osteoinductive media. CMS can serve as building blocks for delivering multiple cell types. Here, hBMSC-loaded and HUVEC-loaded CMS-30, mixed at a 1:1 ratio, are injected subcutaneously into nude mice for 2 months. Results show the development of vascularized bone-like tissue with high levels of OCN and CD31. These findings indicate that GelMA CMS of a certain pore size can effectively deliver multiple cells to achieve functional tissue regeneration.
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http://dx.doi.org/10.1002/smll.202006596DOI Listing
March 2021

Classification of Peritumoral Veins in Convexity and Parasagittal Meningiomas and Its Significance in Preventing Cerebral Venous Infarction.

World Neurosurg 2021 May 20;149:e261-e268. Epub 2021 Feb 20.

Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, People's Republic of China. Electronic address:

Objective: The medium (2-4 cm) convexity located closer to the sinus and parasagittal meningiomas (Sindou type I-Ⅲ) without obvious invasion of the superior sagittal sinus are considered simple to operate on. However, the tumors are often accompanied by the cortical bridging vein. Because of lack of collateral vein circulation in cortical areas, the damage of peritumoral veins will subsequently lead to venous infarction. To avoid the serious complications caused by intraoperative injury of peritumoral veins, it is necessary to define the classification of the progression of peritumoral veins and tumors to guide surgical safety.

Methods: The clinical information of 57 patients with convexity and parasagittal meningiomas was collected and retrospectively analyzed. All patients underwent preoperative magnetic resonance imaging and magnetic resonance venography scanning to observe the imaging characteristics of peritumoral veins and preoperative evaluation. The actual relationship between the tumor and peritumoral vein was observed intraoperatively. Postoperative computed tomography and magnetic resonance imaging were used to determine tumor resection and the presence of venous infarction.

Results: According to preoperative magnetic resonance venography and intraoperative findings, we divided the peritumoral veins into 3 types: type A (n = 33, 57.9%), the vein surrounds the tumor; type B (n = 15, 26.3%), the vein is located on the ventral side of the tumor; and type C (n = 9, 15.8%), the vein is located on the dorsal side of the tumor. Peritumoral vein injury occurred in 6 cases followed by serious complications. Treatments were as follows: 4 cases underwent decompression and 2 cases were treated conservatively. The prognosis Glasgow Outcome Scale (GOS) scores were as follows: 3 cases were score 5 for injury of posterior frontal vein or middle frontal vein, 2 cases were score 3 for injury of the central vein, 1 case was score 1 for death due to injury of the central vein. All cases were followed up for 6 months.

Conclusions: Attention should be paid to the peritumoral vein of special meningiomas. Injured vein in the medial third of superior sagittal sinus carries a high rate of postoperative morbidity. Understanding the type of peritumoral veins preoperatively can be used as a guide in determining the corresponding protective strategy during surgery, which can significantly decrease postoperative disability and improve quality of life.
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http://dx.doi.org/10.1016/j.wneu.2021.02.041DOI Listing
May 2021

Role of ethylene in the regulatory mechanism underlying the abortion of ovules after fertilization in Xanthoceras sorbifolium.

Plant Mol Biol 2021 May 21;106(1-2):67-84. Epub 2021 Feb 21.

Institute of Botany, Chinese Academy of Sciences, Beijing, China.

Key Message: Genes related to the MAPK cascade, ethylene signaling pathway, Pi starvation response, and NAC TFs were differentially expressed between normal and abortive ovules. Receptor-mediated ethylene signal perception and transmission play an important role in regulating fruit and ovule development. Xanthoceras sorbifolium, a small to medium-sized tree endemic to northern China, is an emerging dedicated oilseed crop designed for applications in advanced biofuel, engine oil, and functional food, as well as for pharmaceutical and cosmetic applications. Despite the importance of Xanthoceras seed oil, low seed productivity has constricted commercial exploitation of the species. The abortion of developing seeds (ovules after fertilization) is a major factor limiting fruit and seed production in the plant. To increase fruit and seed yields, a better understanding of the mechanisms underlying the abortion of fertilized ovules is critical. This study revealed differences in nucellus degeneration, endosperm development, and starch grain content between normally and abnormally developing ovules after fertilization. We constructed 6 RNA-sequencing (RNA-seq) libraries from normally and abnormally developing ovules at the onset of their abortion process. Comparative transcriptome analysis between the normal and abnormal ovules identified 818 differentially expressed genes (DEGs). Among DEGs, many genes involved in mitogen-activated protein kinase (MAPK) cascades, ethylene signaling pathway, and NAC transcription factor genes showed up-regulated expression in abnormal ovules. The RNA-seq data were validated using quantitative reverse-transcription PCR. Using virus-induced gene silencing (VIGS) methods, evaluation of an ethylene receptor gene (XsERS) function indicated that the gene was closely related to early development of fruits and seeds. Based on the data presented here, we propose a model for a MAPK-ethylene signaling-NAC2 gene regulatory cascade that plays an important role in the regulation of the ovule abortion process in X. sorbifolium. The present study is imperative for understanding the mechanisms of ovule abortion after fertilization and identifying the critical genes and gene networks involved in determining the fate of ovule development.
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http://dx.doi.org/10.1007/s11103-021-01130-2DOI Listing
May 2021

Intraparenchymal atypical meningioma in the posterior fossa: a case report and literature review.

Br J Neurosurg 2021 Feb 17:1-4. Epub 2021 Feb 17.

Department of Neurosurgery, Tangdu hospital, The Fourth Military Medical University, Xi'an, People's Republic of China.

Intraparenchymal meningiomas without dural attachments are extremely rare. A 32-year-old female adult was admitted to our hospital, complaining of occasional dizziness. The patient had no neurological deficits. MRI demonstrated a lesion with mild edema located in the left cerebellar parenchyma. CT revealed calcification within the mass. Gross total resection was achieved. The histopathological examination indicated that the lesion was an atypical meningioma (WHO-II). We herein report an extremely rare case of an intraparenchymal meningioma located in the left cerebellar hemisphere. The significance of the differential diagnosis of lesions in the cerebellum should be emphasized.
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http://dx.doi.org/10.1080/02688697.2021.1884651DOI Listing
February 2021

Efficacy of scopolamine plus propofol in the treatment of recalcitrant psoriasis: A pilot study.

Dermatol Ther 2021 03 23;34(2):e14866. Epub 2021 Feb 23.

Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Accumulating evidence suggests that botulinum neurotoxins (BoNTs), which inhibit acetylcholine release, can be used for treating plaque psoriasis. The therapeutic effects of scopolamine occur through antagonism of central muscarinic acetylcholine receptors. Thus, scopolamine has potential for the treatment of psoriasis. We aimed to evaluate the efficacy and safety of scopolamine plus propofol for the treatment of recalcitrant psoriasis. Twelve patients with recalcitrant psoriasis were enrolled. Patients received intravenous injection of scopolamine plus propofol for 5 consecutive days per month for a total of 3 months. Clinical efficacy was evaluated using a Psoriasis Area and Severity Index (PASI) score. Efficacy outcome was ≥75% reduction in PASI score (PASI75) from baseline. Two patients were lost to follow-up. At week 8, two of 10 patients (20%) achieved PASI75, and at week 12, seven of 10 (70%) achieved PASI75. Treatment was well tolerated, with no reported adverse events. Our study revealed the efficacy and safety of scopolamine plus propofol for the treatment of recalcitrant psoriasis. Scopolamine plus propofol therapy may be a new treatment for recalcitrant psoriasis.
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http://dx.doi.org/10.1111/dth.14866DOI Listing
March 2021

Keratinized mucosa augmentation guided by double xenogeneic collagen matrix membranes around implants in the posterior mandible: A case report.

Medicine (Baltimore) 2021 Jan;100(3):e23609

School and Hospital of Stomatology, Jilin University, Department of Dental Implantology, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun, Jilin Province, China.

Rationale: Traditional free gingival graft (FGG) technique is usually used for patients with insufficient peri-implant keratinized mucosa. However, this technique often requires a second surgical area which increases the pain as well as the risk of infection in patients. Xenogeneic collagen matrix (XCM) membrane technique can obtain good results for keratinized mucosa increment.

Patient Concerns: The patient was a 66-year-old healthy female with loss of left mandibular first molar and second molar (FDI #36, #37) for 5 years. Two implants were placed submucosally for 3 months with no interference, while a stage II surgery was needed.

Diagnosis: Probing depth measurements suggested that the mesial, medial, and distal widths of buccal keratinized mucosa within the edentulous area were 0.5, 0.5, and 1 mm, respectively, which were insufficient to maintain the health of peri-implant tissues.

Interventions: Keratinized mucosa augmentation guided by XCM membranes was performed to increase the inadequate buccal keratinized mucosa.

Outcomes: After 2 months of healing, the widths of mesial, medial, and distal buccal keratinized mucosa were 4, 3, and 3 mm, respectively, and the thickness of the augmented mucosa was 4 mm. Then a stage II surgery was followed. The patient was satisfied with the outcomes of keratinized mucosa augmentation.

Lessons: Keratinized mucosa augmentation guided by double XCM membrane technique can be applied to cases with keratinized mucosa width within 2 mm around implants.
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http://dx.doi.org/10.1097/MD.0000000000023609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837944PMC
January 2021

Distribution of Malassezia species on the skin of patients with psoriasis.

J Mycol Med 2021 Jun 7;31(2):101111. Epub 2021 Jan 7.

Department of Mycology, Shanghai Dermatology Hospital, 1278 Baode Road, Shanghai, China. Electronic address:

Introduction: Malassezia species can induce the expression of interleukin-17 (IL-17), which plays an important role in the inflammatory and immune response in psoriasis (PS). The purpose of this study was to investigate the Malassezia species composition in patients with PS and healthy individuals and explore the role of Malassezia species in the pathogenesis of PS.

Materials And Methods: A total of 28 patients with PS and 10 age- and sex-matched healthy individuals participated in this study. Specimens collected from the lesional and non-lesional skin of patients with PS and the skin of healthy individuals were analyzed by using nested PCR.

Results: The relative abundance of Malassezia species was 84.96% in healthy subjects, more than twice that in patients with PS (P<0.01). M. restricta (43.09%) and M. globosa (41.38%) were the main Malassezia species in patients with PS followed by M. furfur (4.84%) and M. sympodialis (2.49%). M. sympodialis accounted for 18. 81% of the Malassezia species in healthy subjects, which was nearly eight times higher than in patients with PS (P<0.01). Further, M. furfur was detected both on lesional and non-lesional psoriatic skin, but it was not found on the skin of healthy individuals.

Conclusions: The Malassezia species composition in patients with PS differed from that of healthy individuals. M. restricta and M. globosa were the main Malassezia species in patients with PS.
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http://dx.doi.org/10.1016/j.mycmed.2021.101111DOI Listing
June 2021

Tracing Carbon Nanotubes (CNTs) in Rat Peripheral Nerve Regenerated with Conductive Conduits Composed of Poly(lactide--glycolide) and Fluorescent CNTs.

ACS Biomater Sci Eng 2020 11 15;6(11):6344-6355. Epub 2020 Oct 15.

State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.

Nerve regeneration can be promoted using nerve guide conduits (NGCs). Carbon nanotubes (CNTs) are often used to prepare conductive NGCs, however, the major concern for their applications is the final location of the implanted CNTs in vivo. Herein, photoluminescent multiwalled CNTs (MWCNTs) were prepared and electrospun with poly(lactide--glycolide) (PLGA), followed by shaping into multichannel NGCs for repairing of injured rat sciatic nerve, thereby the distribution of CNTs in vivo could be detected via bioimaging. Photoluminescent MWCNTs (MWCNT-FITC) were prepared by functionalization with poly(glycidyl methacrylate) (PGMA) and fluorescein-isothiocyanate-isomer I (FITC) subsequently. The conductivity of the PLGA/MWCNT-FITC fibers was approx. 10 S/cm at 3 wt % MWCNTs. Compared with PLGA fibers, Schwann cells on PLGA/MWCNT-FITC fibers matured at a faster rate, accordingly, nerve regeneration was promoted by the PLGA/MWCNT-FITC NGC. With a confocal laser scanning microscope and small-animal imaging system, the location of MWCNTs was detected. Alongside the degradation of PLGA, MWCNTs intended to aggregate and were entrapped in the regenerated nerve tissue without migrating into surrounding tissues and other organs (liver, kidneys, and spleen). This study provides a useful characterization method for MWCNTs and the guidance for in vivo applications of MWCNTs in tissue engineering.
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http://dx.doi.org/10.1021/acsbiomaterials.0c01065DOI Listing
November 2020

Multilayer bare stent technique in treating intact mycotic suprarenal aortic aneurysm: a case report.

Eur Heart J Case Rep 2020 Dec 6;4(6):1-5. Epub 2020 Nov 6.

Department of Vascular Surgery, Shanghai Changhai Hospital, Navy Medical University, Shanghai 200082, People's Republic of China.

Background: Invasive aspergillosis (IA) related mycotic aortic aneurysm is rare in immunocompetent patient. The endovascular therapy remains controversial due to potential risk of graft infection, while the suprarenal cases might face catastrophic complications during open surgery.

Case Summary: We presented an IA case with suprarenal abdominal aortic aneurysm confirmed by joint effusion. Multilayer bare stent technique was performed to preserve visceral blood flow and promote aneurysmal thrombus formation, along with antifungal treatment. Two years of follow-up revealed complete aneurysm thrombosis without evidence of infection.

Discussion: Fungal infection that affects the aorta is difficult to recognize due to often negative blood cultures. Close observation is recommended in case of suspicion of mycotic aneurysms. Multilayer bare stent technique can restore luminal laminar blood flow and reduce the risk of infection in intact mycotic suprarenal aortic aneurysm.
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http://dx.doi.org/10.1093/ehjcr/ytaa361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793158PMC
December 2020

Roles of oxygen level and hypoxia-inducible factor signaling pathway in cartilage, bone and osteochondral tissue engineering.

Biomed Mater 2021 Feb 18;16(2):022006. Epub 2021 Feb 18.

State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.

The repair and treatment of articular cartilage injury is a huge challenge of orthopedics. Currently, most of the clinical methods applied in treating cartilage injuries are mainly to relieve pains rather than to cure them, while the strategy of tissue engineering is highly expected to achieve the successful repair of osteochondral defects. Clear understandings of the physiological structures and mechanical properties of cartilage, bone and osteochondral tissues have been established, but the understanding of their physiological heterogeneity still needs further investigation. Apart from the gradients in the micromorphology and composition of cartilage-to-bone extracellular matrixes, an oxygen gradient also exists in natural osteochondral tissue. The response of hypoxia-inducible factor (HIF)-mediated cells to oxygen would affect the differentiation of stem cells and the maturation of osteochondral tissue. This article reviews the roles of oxygen level and HIF signaling pathway in the development of articular cartilage tissue, and their prospective applications in bone and cartilage tissue engineering. The strategies for regulating HIF signaling pathway and how these strategies finding their potential applications in the regeneration of integrated osteochondral tissue are also discussed.
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http://dx.doi.org/10.1088/1748-605X/abdb73DOI Listing
February 2021

Down-regulation of HCP5 inhibits cell proliferation, migration, and invasion through regulating EPHA7 by competitively binding miR-101 in osteosarcoma.

Braz J Med Biol Res 2021 8;54(2):e9161. Epub 2021 Jan 8.

Jingzhou Institute of Technology, College of Textile and Apparel Design and Art, Jingzhou, China.

Patients with osteosarcoma (OS) usually have poor overall survival because of frequent metastasis. Long non-coding RNAs (lncRNAs) have been reported to be associated with tumorigenesis and metastasis. In this study, we investigated the expression and roles of lncRNA human histocompatibility leukocyte antigen (HLA) complex P5 (HCP5) in OS, aiming to provide a novel molecular mechanism for OS. HCP5 was up-regulated both in OS tissues and cell lines and high expression of HCP5 was associated to low survival in OS patients. Down-regulation of HCP5 inhibited cell proliferation, migration, and invasion, suggesting its carcinogenic role in OS. miR-101 was targeted by HCP5 and its expression was decreased in OS. The inhibitor of miR-101 reversed the impact of HCP5 down-regulation on cell proliferation, apoptosis, and metastasis in OS. Ephrin receptor 7 (EPHA7) was proved to be a target of miR-101 and had ability to recover the effects of miR-101 inhibitor in OS. In conclusion, lncRNA HCP5 knockdown suppressed cell proliferation, migration, and invasion, and induced apoptosis through depleting the expression of EPHA7 by binding to miR-101, providing a potential therapeutic strategy of HCP5 in OS.
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http://dx.doi.org/10.1590/1414-431X20209161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798137PMC
February 2021

Hierarchical and heterogeneous hydrogel system as a promising strategy for diversified interfacial tissue regeneration.

Biomater Sci 2021 Mar;9(5):1547-1573

State Key Laboratory of Organic-Inorganic Composites; Beijing Laboratory of Biomedical Materials; Beijing University of Chemical Technology, Beijing 100029, P.R. China.

Despite breakthroughs in tissue engineering, a tremendous problem still lies in repairing the interfacial tissue which connects soft tissue to hard tissue, particularly cartilage-bone, tendon/ligament-bone, and cementum-ligament-bone interfaces. The challenge comes from the complicated biophysical and biochemical characteristics of interfacial tissues, involving a graded variation of chemical components, structures, and mechanical properties as well as a heterogeneous cell distribution from the soft end to the hard end. Accordingly, significant progress has been made in the design of hierarchical and heterogeneous hydrogel systems in order to solve this problem. Advanced programmable technologies, such as 3D printing and microfluidic platforms, have shown potential in constructing templates or scaffolds with tissue-specific characteristics. The structural specialty of the three aforementioned interfacial tissues is summarized in this review. Then the text concentrates on how to utilize different scale hydrogels (from chemical variation, nanoscale, microscale to cellular regulation) to fabricate gradient constructions for regenerating interfacial tissues, together with in vitro and in vivo outcomes. In particular, the fabrication of continuous gradients is highlighted in this review. Promisingly, the versatile designs involved in fabricating hierarchical and heterogeneous hydrogel systems are predicted to tackle the unsolved problems, and this interfacial tissue engineering methodology is expected to expand its use in therapeutic applications.
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http://dx.doi.org/10.1039/d0bm01595dDOI Listing
March 2021

Mangiferin enhances the antifungal activities of caspofungin by destroying polyamine accumulation.

Virulence 2021 12;12(1):217-230

Shanghai Skin Disease Hospital, Tongji University School of Medicine , Shanghai, P.R. China.

The incidence of fungal infections has increased continuously in recent years. Caspofungin (CAS) is one of the first-line drugs for the treatment of systemic fungal infection. However, the emerging CAS-resistant clinical isolates and high economic cost for CAS administration hamper the wide application of this drug. Thus, the combined administration of CAS with other compounds that can enhance the antifungal activity and reduce the dose of CAS has gained more and more attention. In this study, we investigated the effect of mangiferin (MG) on the antifungal activities of CAS. Our results showed that MG acted synergistically with CAS against various ., including CAS-resistant . Moreover, MG could enhance the activity of CAS against biofilm. The synergism of MG and CAS was further confirmed in a mouse model of disseminated candidiasis. To explore the mechanisms, we found that SPE1-mediated polyamine biosynthesis pathway was involved in the fungal cell stress to caspofungin. Treatment of CAS alone could stimulate SPE1 expression and accumulation of polyamines, while combined treatment of MG and CAS inhibited SPE1 expression and destroyed polyamine accumulation, which might contribute to increased oxidative damage and cell death. These results provided a promising strategy for high efficient antifungal therapies and revealed novel mechanisms for CAS resistance.
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http://dx.doi.org/10.1080/21505594.2020.1870079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801120PMC
December 2021

Corrigendum: Integrated Genetics and Micronutrient Data to Inform the Causal Association Between Serum Calcium Levels and Ischemic Stroke.

Front Cell Dev Biol 2020 18;8:634957. Epub 2020 Dec 18.

Department of Neurosurgery and Institute for Functional Brain Disorders, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.

[This corrects the article DOI: 10.3389/fcell.2020.590903.].
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http://dx.doi.org/10.3389/fcell.2020.634957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775735PMC
December 2020

Crash data augmentation using variational autoencoder.

Accid Anal Prev 2021 Mar 25;151:105950. Epub 2020 Dec 25.

Department of Civil, Environmental and Construction Engineering, University of Central Florida, Orlando, FL, 32816, USA. Electronic address:

In this paper, we present a data augmentation technique to reproduce crash data. The dataset comprising crash and non-crash events are extremely imbalanced. For instance, the dataset used in this paper consists of only 625 crash events for over 6.5 million non-crash events. Thus, learning algorithms tend to perform poorly on these datasets. We have used variational autoencoder to encode all the events into a latent space. After training, the model could successfully separate crash and non-crash events. To generate data, we sampled from the latent space containing crash data. The generated data was compared with the real data from different statistical aspects. t-Test, Levene-test and Kolmogrove Smirnov test showed that the generated data was statistically similar to the real data. It was also compared to some of the minority oversampling techniques like SMOTE and ADASYN as well as the GAN framework for generating data. Crash prediction models based on Logistic Regression (LR), Support Vector Machine (SVM) and Artificial Neural Network (ANN) were used to compare the generated data from the different oversampling techniques. Overall, variational autoencoder (VAE) showed excellent results compared to the other data augmentation methods. Specificity is improved by 8% and 4% for VAE-LR and VAE-SVM respectively when compared to SMOTE while the sensitivity is improved by 6% and 5% when compared to ADASYN. Moreover, VAE generated data also helps to overcome the overfitting problem in SMOTE and ADASYN since there is flexibility in choosing the decision boundary.
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http://dx.doi.org/10.1016/j.aap.2020.105950DOI Listing
March 2021

Morphology, Multilocus Phylogeny, and Toxin Analysis Reveal , the First Lethal Species From Benin, West Africa.

Front Microbiol 2020 20;11:599047. Epub 2020 Nov 20.

CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.

Many species of sect. (Fr.) Quél. cause death of people after consumption around the world. , a new species of sect. from Benin, is described here. The taxon represents the first lethal species of sect. known from Benin. Morphology and molecular phylogenetic analyses based on five genes (ITS, nrLSU, , α, and β) revealed that is a distinct species. The species is characterized by its smooth, white pileus sometimes covered by a patchy volval remnant, a bulbous stipe with a white limbate volva, broadly ellipsoid to ellipsoid, amyloid basidiospores, and abundant inflated cells in the volva. Screening for the most notorious toxins by liquid chromatography-high-resolution mass spectrometry revealed the presence of α-amanitin, β-amanitin, and phallacidin in .
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http://dx.doi.org/10.3389/fmicb.2020.599047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7714729PMC
November 2020

Related Factors of Hepatocellular Carcinoma Recurrence Associated With Hyperglycemia After Liver Transplantation.

Transplant Proc 2021 Jan-Feb;53(1):177-192. Epub 2020 Dec 4.

Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China. Electronic address:

Background: Recurrence of hepatocellular carcinoma (HCC) is the main factor affecting the prognosis of patients with HCC undergoing liver transplantation (LT). In this study, we investigated the influencing factors of tumor recurrence and survival after LT for HCC, especially the long-term correlation with elevated fasting blood glucose (FBG).

Methods: Clinical data from 165 patients with HCC after LT in the General Hospital of Southern Theater Command of PLA between January 2013 and December 2016 were retrospectively analyzed. Disease-free survival (DFS) and overall survival (OS) rates, demographic characteristics, tumor characteristics, and surgical and postoperative data were evaluated.

Results: Among 165 patients, 144 completed over 60 months of follow-up; the median follow-up period was more than 36 months. DFS rates were 76.97%, 51.52%, and 34.73% for 1, 3, and 5 years, respectively. The OS rate for 5 years was 40.28%. Independent risk factors for 1-year DFS were maximum tumor diameter >5 cm, age <49 years, and platelet transfusion. Independent risk factors for 3- and 5-year DFS were maximum tumor diameter >5 cm, capsular invasion, and FBG ≥6.1 mmol/L. Independent risk factors for OS were maximum tumor diameter >5 cm, capsular invasion, and FBG ≥6.1 mmol/L.

Conclusion: Elevated FBG after LT for HCC may promote medium- to long-term tumor recurrence and affect OS. Age <49 years, platelet transfusion, maximum tumor diameter, capsular invasion, and microvascular invasion in patients with HCC also impact survival and tumor recurrence after LT.
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http://dx.doi.org/10.1016/j.transproceed.2020.10.027DOI Listing
April 2021

Integrated Genetics and Micronutrient Data to Inform the Causal Association Between Serum Calcium Levels and Ischemic Stroke.

Front Cell Dev Biol 2020 11;8:590903. Epub 2020 Nov 11.

Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

There has been an increased interest for observational studies or randomized controlled trials exploring the impact of calcium intake on cardiovascular diseases (CVD) including coronary artery disease (CAD) and ischemic stroke (IS). However, a direct relationship between total calcium intake and CVD has not been well established and remains controversial. Mendelian randomization (MR) studies have been performed to evaluate the causal association between serum calcium levels and CAD risk and found that increased serum calcium levels could increase the risk of CAD. However, MR analysis found no significant association between genetically higher serum calcium levels and IS as well as its subtypes. Hence, three MR studies reported inconsistent effects of serum calcium levels on CAD and IS. Here, we performed an updated MR study to investigate the association of serum calcium levels with the risk of IS using large-scale genome-wide association study (GWAS) datasets. We selected 14 independent genetic variants as the potential instrumental variables from a large-scale serum calcium GWAS dataset and extracted summary statistics corresponding to the 14 serum calcium genetic variants from the MEGASTROKE Consortium IS GWAS dataset. Interestingly, we found a significant association between serum calcium levels and IS risk using the robust inverse-variance weighted (IVW) and penalized robust IVW methods, with β = 0.243 and = 0.002. Importantly, the MR results from the robust MR-Egger and penalized robust MR-Egger methods further supported the causal association between serum calcium levels and IS risk, with β = 0.256 and = 0.005. Meanwhile, the estimates from other MR methods are also consistent with the above findings.
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http://dx.doi.org/10.3389/fcell.2020.590903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686510PMC
November 2020