Publications by authors named "Qin Yang"

974 Publications

Homocysteine alters vasoreactivity of human internal mammary artery by affecting the K channel family.

Ann Transl Med 2021 Apr;9(8):625

Center for Basic Medical Research & Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

Background: Hyperhomocysteinemia is an independent risk factor for atherosclerotic heart disease. We previously demonstrated that disruption of calcium-activated potassium (K) channel activity is involved in homocysteine-induced dilatory dysfunction of porcine coronary arteries. Recently we reported that the K channel family, including large-, intermediate-, and small-conductance K (BK, IK, and SK) subtypes, are abundantly expressed in human internal mammary artery (IMA). In this study, we further investigated whether homocysteine affects the expression and functionality of the K channel family in this commonly used graft for coronary artery bypass surgery (CABG).

Methods: Residual IMA segments obtained from patients undergoing CABG were studied in a myograph for the role of K subtypes in both vasorelaxation and vasoconstriction. The expression and distribution of K subtypes were detected by Western blot and immunohistochemistry.

Results: Both the BK channel activator NS1619 and the IK/SK channel activator NS309 evoked significant IMA relaxation. Homocysteine exposure suppressed NS1619-induced relaxation whereas showed no influence on NS309-induced response. Blockade of BK but not IK and SK subtypes significantly suppressed acetylcholine-induced relaxation and enhanced U46619-induced contraction. Homocysteine compromised the vasodilating activity of the BK subtype in IMA, associated with a lowered protein level of the BK β1-subunit. Homocysteine potentiated the role of IK and SK subtypes in mediating endothelium-dependent relaxation without affecting the expression of these channels.

Conclusions: Homocysteine reduces the expression of BK β1-subunit and compromises the vasodilating activity of BK channels in IMA. Unlike BK, IK and SK subtypes are unessential for IMA vasoregulation, whereas the loss of BK functionality in hyperhomocysteinemia enhances the role of IK and SK subtypes in mediating endothelial dilator function. Targeting BK channels may form a strategy to improve the postoperative graft performance in CABG patients with hyperhomocysteinemia who receive IMA grafting.
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http://dx.doi.org/10.21037/atm-20-6821DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106027PMC
April 2021

CTHRC1 promotes liver metastasis by reshaping infiltrated macrophages through physical interactions with TGF-β receptors in colorectal cancer.

Oncogene 2021 May 13. Epub 2021 May 13.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Metastasis is a major cause of cancer-related deaths. Tumor-intrinsic properties can determine whether tumor metastasis occurs or not. Here, by comparing the gene expression patterns in primary colorectal cancer (CRC) patients with or without metastasis, we found that Collagen Triple Helix Repeat Containing 1 (CTHRC1) in primary CRC served as a metastasis-associated gene. Animal experiments verified that CTHRC1 secreted by CRC cells promoted hepatic metastasis, which was closely correlated with macrophage infiltration. Depletion of macrophages by liposomal clodronate largely abolished the promoting effect of CTHRC1 on CRC liver metastasis. Furthermore, we demonstrated that CTHRC1 modulated macrophage polarization to M2 phenotypes through TGF-β signaling. A mechanistic study revealed that CTHRC1 bound directly to TGF-β receptor II and TGF-β receptor III, stabilized the TGF-β receptor complex, and activated TGF-β signaling. The combination treatment of CTHRC1 monoclonal antibody and anti-PD-1 blocking antibody effectively suppressed CRC hepatic metastasis. Taken together, our data demonstrated that CTHRC1 is an intrinsic marker of CRC metastasis and further revealed that CTHRC1 promoted CRC liver metastasis by reshaping infiltrated macrophages through TGF-β signaling, suggesting that CTHRC1 could be a potential biomarker for the early prediction of and a therapeutic target of CRC hepatic metastasis.
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http://dx.doi.org/10.1038/s41388-021-01827-0DOI Listing
May 2021

Comprehensive and high-resolution emission inventory of atmospheric pollutants for the northernmost cities agglomeration of Harbin-Changchun, China: Implications for local atmospheric environment management.

J Environ Sci (China) 2021 Jun 18;104:150-168. Epub 2020 Dec 18.

Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.

Using a bottom-up estimation method, a comprehensive, high-resolution emission inventory of gaseous and particulate atmospheric pollutants for multiple anthropogenic sectors with typical local sources has been developed for the Harbin-Changchun city agglomeration (HCA). The annual emissions for CO, NOx, SO, NH, VOC, PM, PM, BC and OC during 2017 in the HCA were estimated to be 5.82 Tg, 0.70 Tg, 0.34 Tg, 0.75 Tg, 0.81Tg, 0.67 Tg, 1.59 Tg, 0.12 Tg and 0.26 Tg, respectively. For PM and SO, the emissions from industry processes were the dominant contributors representing 54.7% and 49.5%, respectively, of the total emissions, while 95.3% and 44.5% of the total NH and NOx emissions, respectively, were from or associated with agricultural activities and transportation. Spatiotemporal distributions showed that most emissions (except NH) occurred in November to March and were concentrated in the central cities of Changchun and Harbin and the surrounding cities. Open burning of straw made an important contribution to PM in the central regions of the northeastern plain during autumn and spring, while domestic coal combustion for heating purposes was significant with respect to SO and PM emissions during autumn and winter. Furthermore, based on Principal Component Analysis and Multivariable Linear Regression model, air temperature, relative humidity, electricity and energy consumption, and the urban and rural population were optimized to be representative indicators for rapidly assessing the magnitude of regional atmospheric pollutants in the HCA. Such indicators and equations were demonstrated to be useful for local atmospheric environment management.
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http://dx.doi.org/10.1016/j.jes.2020.11.026DOI Listing
June 2021

Prostaglandin E attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization.

J Cell Mol Med 2021 May 13. Epub 2021 May 13.

Wuxi School of Medicine, Jiangnan University, Wuxi, China.

Alternative polarization of macrophages regulates multiple biological processes. While M1-polarized macrophages generally mediate rapid immune responses, M2-polarized macrophages induce chronic and mild immune responses. In either case, polyunsaturated fatty acid (PUFA)-derived lipid mediators act as both products and regulators of macrophages. Prostaglandin E (PGE ) is an eicosanoid derived from eicosapentaenoic acid, which is converted by cyclooxygenase, followed by prostaglandin E synthase successively. We found that PGE played an anti-inflammatory role by inhibiting LPS and interferon-γ-induced M1 polarization and promoting interleukin-4-mediated M2 polarization (M2a). Further, we found that although PGE had no direct effect on the growth of prostate cancer cells in vitro, PGE could inhibit prostate cancer in vivo in a nude mouse model of neoplasia. Notably, we found that PGE significantly inhibited prostate cancer cell growth in a cancer cell-macrophage co-culture system. Experimental results showed that PGE inhibited the polarization of tumour-associated M2 macrophages (TAM), consequently producing indirect anti-tumour activity. Mechanistically, we identified that PGE regulated the expression and activation of protein kinase A, which is critical for macrophage polarization. In summary, this study indicates that PGE can selectively promote M2a polarization, while inhibiting M1 and TAM polarization, thus exerting an anti-inflammatory effect and anti-tumour effect in prostate cancer.
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http://dx.doi.org/10.1111/jcmm.16570DOI Listing
May 2021

gen. et sp. nov., a new diaporthalean fungus from Chinese chestnut branches in southern China.

MycoKeys 2021 16;79:1-16. Epub 2021 Apr 16.

The Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing 100083, China Beijing Forestry University Beijing China.

-like fungi are distributed in several families of Diaporthales, mainly Juglanconidaceae, Melanconidaceae, Melanconiellaceae and Pseudomelanconidaceae. A new -like genus of Pseudomelanconidaceae was discovered on branches of Chinese chestnut () in southern China, which was confirmed by both morphology and phylogenetic analysis of combined ITS, LSU, and sequences. The new genus is characterized by two types of conidia from natural substrate and manual media of PDA, respectively. Conidia from Chinese chestnut branches are pale brown, ellipsoid, multiguttulate, aseptate with hyaline sheath. While conidia from PDA plates are pale brown, long dumbbell-shaped, narrow at the middle and wide at both ends, multiguttulate, aseptate, and also with hyaline sheath. All Pseudomelanconidaceae species were only reported on tree branches in China until now. More interesting taxa may be discovered if detailed surveys on tree-inhabiting fungi are carried out in East Asia in the future.
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http://dx.doi.org/10.3897/mycokeys.79.65221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065008PMC
April 2021

Irisin ameliorates endoplasmic reticulum stress and liver fibrosis through inhibiting PERK-mediated destabilization of HNRNPA1 in hepatic stellate cells.

Biol Chem 2021 May 20;402(6):703-715. Epub 2021 Jan 20.

Department of Pathophysiology, Guizhou Medical University, No. 9 Beijing Road, Yunyan District, Guiyang City550004, Guizhou Province, China.

Liver fibrosis is a common consequence of chronic liver diseases involved with the activation of hepatic stellate cells (HSCs) and endoplasmic reticulum (ER) stress. Irisin is a small polypeptide hormone that shows beneficial effects on metabolic disorders. The current study aimed to investigate the biological function of irisin on hepatic fibrosis. A mouse model of carbon tetrachloride (CCl)-induced hepatic fibrosis was established. CCl-treated mice showed elevated serum levels of AST and ALT, increased collagen accumulation, induced ER stress, and upregulated expressions of pro-fibrotic proteins in the liver compared to the controls. The administration of irisin, however, ameliorated CCl-induced hepatic fibrosis in both cultured HSCs and mice. PKR-like ER kinase (PERK) is a key component of the ER stress-associated signaling pathway. We found that irisin treatment improved the stability of heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) via regulating the phosphorylation of PERK in mouse livers and isolated HSCs. Also, the knockdown of HNRNPA1 eliminated the hepatoprotective effects of irisin on hepatic fibrosis and ER stress. In summary, this study showed that irisin alleviated ER stress and hepatic fibrosis by inhibiting PERK-mediated HNRNPA1 destabilization, suggesting that irisin may represent a promising therapeutic strategy for patients with liver fibrosis.
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http://dx.doi.org/10.1515/hsz-2020-0251DOI Listing
May 2021

Exploring the bacterial community for starters in traditional high-salt fermented Chinese fish (Suanyu).

Food Chem 2021 Apr 20;358:129863. Epub 2021 Apr 20.

School of Liquor and Food Engineering, Guizhou University, Guiyang, China; Guizhou Provincial Key Laboratory of Agricultural and Animal Products Storage and Processing, Guiyang, China; Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guiyang, China. Electronic address:

Traditional high-salt fermented Suanyu is an ethnic fermented fish product in southwest China. Lactic acid bacteria (LAB) are the most appropriate strains because of their technological properties during ripening fermentation. The diversity of LAB in high-salt fermented Chinese Suanyu was examined through high-throughput sequencing (HTS), and the most suitable LAB strain was acquired through strain isolation and characterization, surimi simulation fermentation system, and principal component analysis (PCA). The processing adaptability of the strain was examined via Suanyu fermentation. Results showed that Lactobacillus, Tetragenococcus, and Weissella were the dominant bacteria in Suanyu, and their contributions were 53.99%, 35.60%, and 4.10%, respectively. The most suitable strain (Lactobacillus plantarum B7) rapidly produced acid, exhibited a strong antibacterial activity, showed salt tolerance, and had no amino acid decarboxylase activity. pH decreased to about 3.6. Eventually, the ability to tolerate 20% salt was observed, and the activity of amino acid decarboxylase was negative. Fermented Suanyu with B7 rapidly produced acid (11.7% d). The non-protein nitrogen (NPN) and total free amino acid (FAA) contents of fermented Suanyu were higher and its total volatile base nitrogen (TVB-N), thiobarbituric acid (TBARS), and biogenic amines (BAs) levels were lower than those of naturally fermented Suanyu. Therefore, B7 is a potential microbial starter for Suanyu industrial production.
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http://dx.doi.org/10.1016/j.foodchem.2021.129863DOI Listing
April 2021

Feeding state greatly modulates the effect of xenobiotics on gut microbiome metabolism: A case study of tetracycline.

J Hazard Mater 2021 Jul 16;413:125441. Epub 2021 Feb 16.

School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798; Nanyang Environment and Water Research Institute, Nanyang Technological University, Singapore 637141; Singapore Phenome Center, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 636921. Electronic address:

The human gut microbiome is crucial in modulating host health mostly through bacterial metabolites. Chemical exposure is typical external stress which alters its composition and functionality. To date, very few studies have investigated the effect of feeding state on chemical-induced gut microbial metabolic dysregulations. Here, we set up an in vitro human gut microbiome and incorporated a metabolomics approach to investigate the effect of tetracycline (TET) at multiple doses (i.e., 10, 1, and 0.01 mg/L) on gut microbiome under the fed and fasted states. Overall, the metabolome was highly responsive at the fed state with 62 metabolites dysregulated while only 14 were altered at the fasted state under 10 mg/L (clinical TET dose). As expected, nutrient consumption was significantly inhibited under clinical TET dose at the fed state accumulating nutrients such as glutamate and leucine. Interestingly, at the fed state, TET could increase the synthesis of indole and phenyl derivatives including indole-3-aldehyde and hydrocinnamate, while inhibiting indoxyl, tryptamine, and vitamin B production, all of which have host health implications. Furthermore, metabolites like indoxyl and xanthurenic acid were still responsive at 0.01 mg/L (dietary TET dose). Collectively, results demonstrated that the feeding state greatly modulates the chemical-induced gut microbial metabolic alterations.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125441DOI Listing
July 2021

Landscape Pattern Evolution Processes of Wetlands and Their Driving Factors in the Xiong'an New Area of China.

Int J Environ Res Public Health 2021 Apr 21;18(9). Epub 2021 Apr 21.

College of Water Science and Engineering, Zhengzhou University, Zhengzhou 450001, China.

Wetland landscape patterns are the result of various ecological and hydrological processes. Based on the land use landscape types from 1980 to 2017, a transfer matrix, landscape pattern analysis index, and principal component analysis were used to analyze the landscape pattern evolution in the Xiong'an New Area of China, which has a large area with a lake and river wetlands. The results showed that the wetland area has changed greatly since 2000 and the beach land has decreased greatly, while the area of the lake and river wetlands has increased slightly. Beach land was the dominant landscape type of the wetland. The dominant degree of the wetland landscape showed a slightly decreasing trend, and the patches tended to be scattered. The shape complexity of the ponds was the lowest, while that of rivers was the highest. The fragmentation degree of the wetland patches increased, the proportion of landscape types tended to be equalized, and the landscape heterogeneity increased. The leading factors of the wetland landscape change can be summarized as socioeconomic, meteorological, and hydrological processes, with a cumulative contribution rate of 85.3%, among which socioeconomic development was the most important factor. The results have important guiding significance for the ecological restoration and management of wetlands in the Xiong'an New Area and other wetland ecosystems with rivers and lakes.
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http://dx.doi.org/10.3390/ijerph18094403DOI Listing
April 2021

Rho/ROCK-MYOCD in regulating airway smooth muscle growth and remodeling.

Authors:
Qin Yang Wei Shi

Am J Physiol Lung Cell Mol Physiol 2021 Apr 28. Epub 2021 Apr 28.

Department of Surgery, Children's Hospital of Los Angeles, United States.

Abnormal airway remodeling is a common pathologic change seen in chronic respiratory diseases. Altered proliferation and differentiation of airway smooth muscle cells (ASMCs) are the major component of airway remodeling, and the resultant structural abnormalities are difficult to restore. Understanding of airway smooth muscle regulation is urgently needed in order to identify potential intervention targets. MYOCD (or Myocardin) and Myocardin related transcription factors (MRTFs) are key co-transcription factors in muscle growth, which have not been extensively investigated in airway smooth muscle cells. In addition, the RhoA/ROCK signaling pathway is known to play an important role in airway remodeling partly through regulating the proliferation and differentiation of ASMCs, which may be connected with MYOCD/MRTF co-transcription factors. This minireview focuses on this newly recognized and potentially important RhoA/ROCK-MYOCD/MRTFs pathway in controlling airway smooth muscle growth and remodeling.
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http://dx.doi.org/10.1152/ajplung.00034.2021DOI Listing
April 2021

Inhibition of receptor-interacting protein kinase-3 in the necroptosis pathway attenuates inflammatory bone loss in experimental apical periodontitis in Balb/c mice.

Int Endod J 2021 Apr 25. Epub 2021 Apr 25.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Sichuan, China.

Aim: To explore the role of necroptosis in apical periodontitis (AP), this study investigated necroptosis in a Fusobacterium nucleatum (Fn)-induced AP model of Balb/c mice and explored related intracellular signalling pathways in L929 cells affected by Fn.

Methodology: For the in vivo experiments, expression of receptor-interacting protein kinase-3 (RIP3) was inhibited using an adeno-associated virus and then the Balb/c mice model of AP was established by injecting Fn into the root canal of the first mandibular molars. Bone loss and number of osteoclasts were measured via micro-computed tomography and tartrate-resistant acid phosphatase staining, respectively; expression of RIP3 and phosphorylated mixed lineage kinase domain-like protein (pMLKL) was detected by immunohistochemistry and western blotting; expression of mRNA of inflammatory cytokines was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). For the in vitro experiments, L929 cells transfected with RIP3-Mus-siRNA or negative control siRNA were co-cultured with Fn; thereafter, western blotting, detection of cell death and viability and qRT-PCR analyses were performed to assess the activation of necroptosis pathway and expression of mRNA of inflammatory cytokines. Data were analysed with unpaired t-test and one-way analysis of variance with significance set at p < .05.

Results: The Fn-induced apical lesions were associated with apical bone loss, an increased number of osteoclasts, enhanced expression of pMLKL and increased mRNA levels of inflammatory cytokines(IL-1α and IL-1β); all these effects were alleviated by RIP3 inhibition (p < .05). L929 cells infected with Fn displayed increased expression of pMLKL and increased cell death (p < .05), together with decreased cell viability (p < .05), whilst transfection with RIP3-Mus-siRNA decreased the mRNA expression of inflammatory cytokines(TNF-α and IL-6, p < .05).

Conclusions: Necroptosis may be involved in AP progression. RIP3 inhibition ameliorated the expression of inflammatory cytokines and bone resorption in Fn-induced AP lesions in Balb/c mice.
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http://dx.doi.org/10.1111/iej.13534DOI Listing
April 2021

Machine learning guided aptamer refinement and discovery.

Nat Commun 2021 04 22;12(1):2366. Epub 2021 Apr 22.

Aptitude Medical Systems Inc., Santa Barbara, CA, USA.

Aptamers are single-stranded nucleic acid ligands that bind to target molecules with high affinity and specificity. They are typically discovered by searching large libraries for sequences with desirable binding properties. These libraries, however, are practically constrained to a fraction of the theoretical sequence space. Machine learning provides an opportunity to intelligently navigate this space to identify high-performing aptamers. Here, we propose an approach that employs particle display (PD) to partition a library of aptamers by affinity, and uses such data to train machine learning models to predict affinity in silico. Our model predicted high-affinity DNA aptamers from experimental candidates at a rate 11-fold higher than random perturbation and generated novel, high-affinity aptamers at a greater rate than observed by PD alone. Our approach also facilitated the design of truncated aptamers 70% shorter and with higher binding affinity (1.5 nM) than the best experimental candidate. This work demonstrates how combining machine learning and physical approaches can be used to expedite the discovery of better diagnostic and therapeutic agents.
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http://dx.doi.org/10.1038/s41467-021-22555-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062585PMC
April 2021

The pan-cancer noninvasive screening and early detection by epigenetic techniques.

Epigenomics 2021 May 20;13(9):649-652. Epub 2021 Apr 20.

Department of Radiotherapy, The Eighth Medical Center of The Chinese PLA General Hospital, Beijing 100091, PR China.

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http://dx.doi.org/10.2217/epi-2021-0063DOI Listing
May 2021

A novel plasmid from Aerococcus urinaeequi of porcine origin co-harboring the tetracycline resistance genes tet(58) and tet(61).

Vet Microbiol 2021 Apr 9;257:109065. Epub 2021 Apr 9.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. Electronic address:

Tetracyclines are the broad-spectrum agents used in veterinary medicine and food animal production. Known mechanisms of tetracycline resistance include ribosome protection, active efflux and enzymatic inactivation. However, the presence of two different tet genes conferring different resistance mechanisms on the same plasmid has rarely been reported. In this study, we identified the tandem tetracycline resistance genes tet(61)-tet(58) on the novel plasmid pT4303. These tet genes were identified for the first time in Aerococcus urinaeequi. Reduced susceptibility to doxycycline was observed in S. aureus RN4220 harboring tet(61) when an extra tet(58) was expressed. Plasmid pT4303 was electrotransformed into S. aureus RN4220, E. faecalis JH2-2, S. suis BAA and E. coli DH5α and conferred tetracycline resistance (MIC ≥ 16) in both Gram-positive and Gram-negative bacteria, assuming that it might serve as a vehicle for the dissemination of the tetracycline resistance genes tet(61) and tet(58).
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http://dx.doi.org/10.1016/j.vetmic.2021.109065DOI Listing
April 2021

Norepinephrine Enhances Aerobic Glycolysis and May Act as a Predictive Factor for Immunotherapy in Gastric Cancer.

J Immunol Res 2021 27;2021:5580672. Epub 2021 Mar 27.

Department of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

Methods: Monoamine neurotransmitters were detected in gastric cancer tissue and paired normal tissue, and The Cancer Genome Atlas was used to identify differentially expressed norepinephrine-degrading and synthetic enzymes. Quantitative real-time PCR and the Seahorse assay were used to determine the effect of norepinephrine on gastric cancer cell glycolysis. MAOA expression in cancer tissues was analyzed by immunohistochemistry and was compared with the patient SUVmax value of PET-CT and other clinicopathological characteristics.

Results: The norepinephrine levels were markedly high in gastric cancer tissue, while the norepinephrine-degrading enzymes MAOA and MAOB showed low expression. High norepinephrine levels were associated with activated glycolysis. The MAOA or MAOB expression levels in tumor tissue were closely correlated with the patient SUV max values of PET-CT and immunotherapy evaluation indices, such as PD-L1 and the microsatellite status.

Conclusions: Norepinephrine shows relatively higher expression in gastric cancer tissue than in normal tissue, and its expression level is associated with the glycolysis levels in patients. The norepinephrine-degrading enzymes MAOA and MAOB have significant expression differences in cancer and normal tissue, and their missing or low expression may predict immune therapy outcomes for gastric cancer patients. High norepinephrine levels with metabolic abnormalities may be more suitable for metabolic targeted therapy or immunotherapy.
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http://dx.doi.org/10.1155/2021/5580672DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019630PMC
March 2021

Factors associated with a SARS-CoV-2 recurrence after hospital discharge among patients with COVID-19: systematic review and meta-analysis.

J Zhejiang Univ Sci B 2020 Dec.;21(12):940-947

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.

Background: The proportion of recurrences after discharge among patients with coronavirus disease 2019 (COVID-19) was reported to be between 9.1% and 31.0%. Little is known about this issue, however, so we performed a meta-analysis to summarize the demographical, clinical, and laboratorial characteristics of non-recurrence and recurrence groups.

Methods: Comprehensive searches were conducted using eight electronic databases. Data regarding the demographic, clinical, and laboratorial characteristics of both recurrence and non-recurrence groups were extracted, and quantitative and qualitative analyses were conducted.

Results: Ten studies involving 2071 COVID-19 cases were included in this analysis. The proportion of recurrence cases involving patients with COVID-19 was 17.65% (between 12.38% and 25.16%) while older patients were more likely to experience recurrence (weighted mean difference (WMD)=1.67, range between 0.08 and 3.26). The time from discharge to recurrence was 13.38 d (between 12.08 and 14.69 d). Patients were categorized as having moderate severity (odds ratio (OR)=2.69, range between 1.30 and 5.58), while those with clinical symptoms including cough (OR=5.52, range between 3.18 and 9.60), sputum production (OR=5.10, range between 2.60 and 9.97), headache (OR=3.57, range between 1.36 and 9.35), and dizziness (OR=3.17, range between 1.12 and 8.96) were more likely to be associated with recurrence. Patients presenting with bilateral pulmonary infiltration and decreased leucocyte, platelet, and CD4 T counts were at risk of COVID-19 recurrence (OR=1.71, range between 1.07 and 2.75; WMD=-1.06, range between -1.55 and -0.57, WMD=-40.39, range between -80.20 and -0.48, and WMD=-55.26, range between -105.92 and -4.60, respectively).

Conclusions: The main factors associated with the recurrence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) after hospital discharge were older age, moderate severity, bilateral pulmonary infiltration, laboratory findings including decreased leucocytes, platelets, and CD4 T counts, and clinical symptoms including cough, sputum production, headache, and dizziness. These factors can be considered warning indicators for the recurrence of SARS-CoV-2 and might help the development of specific management strategies.
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http://dx.doi.org/10.1631/jzus.B2000304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759453PMC
April 2021

Biomarkers and key pathways in atrial fibrillation associated with mitral valve disease identified by multi-omics study.

Ann Transl Med 2021 Mar;9(5):393

Center for Basic Medical Research & Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences & Graduate School, Peking Union Medical College, Beijing, China.

Background: Mitral valve disease (MVD)-associated atrial fibrillation (AF) is one of the most common arrhythmias with an increased risk of thromboembolic events. This study aimed to identify the molecular mechanisms and possible biomarkers for chronic AF in MVD by using multi-omics methods.

Methods: This prospective study enrolled patients with MVD (n=100) undergoing mitral valve replacement surgery. The patients were allocated into chronic AF and sinus rhythm (SR) groups. Plasma samples were collected preoperatively. Proteomics was performed with isobaric tags for relative and absolute quantitation (iTRAQ) to identify differential proteins (DPs) between the two groups. The selected DPs were then validated in a new cohort of patients by enzyme-linked immunosorbent assay (ELISA). A gas chromatography-mass spectrometer was used in the metabolomics study to identify differential metabolites (DMs). Bioinformatics analyses were performed to analyze the results.

Results: Among the 447 plasma proteins and 322 metabolites detected, 57 proteins and 55 metabolites, including apolipoprotein A-I (ApoA-I), apolipoprotein A-II (ApoA-II), LIM domain only protein 7 (LMO7), and vitronectin (VN) were differentially expressed between AF and SR patients. Bioinformatics analyses identified enriched pathways related to AF, including peroxisome proliferator-activated receptor alpha (PPARα), the renin angiotensin aldosterone system (RAAS), galactose, biosynthesis of unsaturated fatty acids, and linoleic acid metabolism.

Conclusions: Using integrated multi-omics technologies in MVD-associated AF patients, the present study, for the first time, revealed important signaling pathways, such as PPARα, as well as possible roles of other signaling pathways, including the RAAS and galactose metabolism to understand the molecular mechanism of MVD-associated AF. It also identified a large number of DPs and DMs. Some identified proteins and metabolites, such as ApoA-I, ApoA-II, LMO7, and VN, may be further developed as biomarkers for MVD-associated AF.
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http://dx.doi.org/10.21037/atm-20-3767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033373PMC
March 2021

Inhibition of regulator of G protein signaling 10, aggravates rheumatoid arthritis progression by promoting NF-κB signaling pathway.

Mol Immunol 2021 Jun 6;134:236-246. Epub 2021 Apr 6.

The State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Sichuan, People's Republic of China. Electronic address:

Rheumatoid arthritis (RA) is the most common inflammatory arthropathy, with evidence pointing to an immune-mediated etiology that propagates chronic inflammation. Although targeted immune therapeutics and aggressive treatment strategies have substantially improved, a complete understanding of the associated pathological mechanisms of the disease remains elusive. This study aimed at investigating whether regulator of G protein signaling 10 (RGS10) could affect rheumatoid arthritis (RA) pathology by regulating the immune response. A DBA/J1 mouse model of RA was established and evaluated for disease severity. RGS10 expression was inhibited by adeno-associated virus in vivo. Moreover, small interfering RNA was used to downregulate RGS10 expression in raw 264.7 cells in vitro. Results showed that RGS10 inhibition augmented RA severity, and attenuated the increase in expression of inflammatory factors. Furthermore, activated NF-κB signaling pathways were detected following RGS10 inhibition. These results revealed that RGS10 inhibition directly aggravated the RA pathological process by activating the NF-κB signaling pathway. Therefore, RGS10 is a promising novel therapeutic target for RA treatment with a potential clinical impact.
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http://dx.doi.org/10.1016/j.molimm.2021.03.024DOI Listing
June 2021

Baicalin Represses Type Three Secretion System of through PQS System.

Molecules 2021 Mar 10;26(6). Epub 2021 Mar 10.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi'an 710069, China.

Therapeutics that target the virulence of pathogens rather than their viability offer a promising alternative for treating infectious diseases and circumventing antibiotic resistance. In this study, we searched for anti-virulence compounds against from Chinese herbs and investigated baicalin from as such an active anti-virulence compound. The effect of baicalin on a range of important virulence factors in was assessed using -based reporters and by phenotypical assays. The molecular mechanism of the virulence inhibition by baicalin was investigated using genetic approaches. The impact of baicalin on pathogenicity was evaluated by both in vitro assays and in vivo animal models. The results show that baicalin diminished a plenty of important virulence factors in , including the Type III secretion system (T3SS). Baicalin treatment reduced the cellular toxicity of on the mammalian cells and attenuated in vivo pathogenicity in a infection model. In a rat pulmonary infection model, baicalin significantly reduced the severity of lung pathology and accelerated lung bacterial clearance. The PqsR of the quinolone signal (PQS) system was found to be required for baicalin's impact on T3SS. These findings indicate that baicalin is a promising therapeutic candidate for treating infections.
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http://dx.doi.org/10.3390/molecules26061497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001617PMC
March 2021

Generation and identification of endothelial-specific Hrh2 knockout mice.

Transgenic Res 2021 Mar 30. Epub 2021 Mar 30.

Department of Clinical Pharmacy, 920th Hospital of Joint Logistics Support Force, 212 Daguan Rd, Kunming, 650032, China.

Histamine H receptor (HRH2) is closely associated with the development of cardiovascular and cerebrovascular diseases. However, systematic Hrh2 knockout mice did not exactly reflect the HRH2 function in specific cell or tissue types. To better understand the physiological and pathophysiological functions of endothelial HRH2, this study constructed a targeting vector that contained loxp sites flanking the ATG start codon located in Hrh2 exon 2 upstream and a neomycin (Neo) resistance gene flanked by self-deletion anchor sites within the mouse Hrh2 allele. The targeting vector was then electroporated into C57BL/6J embryonic stem (ES) cells, and positively targeted ES cell clones were micoinjected into C57BL/6J blastocysts, which were implanted into pseudopregnant females to obtain chimeric mice. The F1 generation of Hrh2 mice was generated via crossing chimeric mice with wild-type mice to excise Neo. We also successfully generated endothelial cell-specific knockout (ECKO) mice by crossing Hrh2 mice with Cdh5-Cre mice that specifically express Cre in endothelial cells and identified that Hrh2 deletion was only observed in endothelial cells. Hrh2 and Hrh2 mice were normal, healthy and fertile and did not display any obvious abnormalities. These novel animal models will create new prospects for exploring roles of HRH2 during the development and treatment of related diseases.
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http://dx.doi.org/10.1007/s11248-021-00244-zDOI Listing
March 2021

Meibum lipid composition in type 2 diabetics with dry eye.

Exp Eye Res 2021 May 26;206:108522. Epub 2021 Mar 26.

Department of Ophthalmology,Yangpu Hospital, Tongji University School of Medicine, Shanghai, 20090, China. Electronic address:

Purpose: The purpose of this investigation was to analyze and compare the composition of meibum between type 2 diabetics with dry eye disease (DED) and control subjects to better reveal the pathologic mechanisms of the meibomian gland degeneration (MGD) and DED in type 2 diabetes mellitus (T2DM).

Methods: 90 subjects were divided into the following 4 groups: DM-DED group: T2DM patients with DED (n = 30); DM control group: DM patients without DED (n = 18); DED group: DED patients without DM (n = 26); naive control group: normal subjects (n = 16). The lipid composition of meibum samples collected from these subjects was analyzed by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) system. The content of lipid features from 12 major lipid classes was compared among the 4 groups.

Results: A significantly lower level of triacylglycerols (TG) and wax esters (WE) was found between DM-DED patients and normal controls (P < 0.01), whereas the level of Cholesteryl Ester (CE) in DM-DED patients increased compared with DED patients (P < 0.05). The level of (O-acyl)-omega-hydroxy fatty acids (OAHFA) in DM-DED patients was significantly lower than that in normal controls (P < 0.01). An opposite higher level of phospholipids (PLs) was observed in DM-DED patients than that in normal controls (P < 0.01).

Conclusions: T2DM could influence the expression of meibum lipids to further aggravate DED and MGD. Lower expression of TG,WE and OAHFA, higher expression of CE and PLs were discovered in meibum lipids of T2DM-DED.
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http://dx.doi.org/10.1016/j.exer.2021.108522DOI Listing
May 2021

Gypenoside XLIX protects against acute kidney injury by suppressing IGFBP7/IGF1R-mediated programmed cell death and inflammation.

Phytomedicine 2021 May 5;85:153541. Epub 2021 Mar 5.

Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, the Key Laboratory of Anti-inflammatory of Immune Medicines, Ministry of Education, Hefei, 230032, China. Electronic address:

Background: Acute kidney injury (AKI), characterised by excessive inflammatory cell recruitment and programmed cell death, has a high morbidity and mortality; however, effective and specific therapies for AKI are still lacking.

Objective: This study aimed to evaluate the renoprotective effects of gypenoside XLIX (Gyp XLIX) in AKI.

Methods: The protective effects of Gyp XLIX were tested in two AKI mouse models established using male C57BL/6 mice (aged 6-8 weeks) by a single intraperitoneal injection of cisplatin (20 mg/kg) or renal ischemia-reperfusion for 40 min. Gyp XLIX was administered intraperitoneally before cisplatin administration or renal ischemia-reperfusion. Renal function, tubular injury, renal inflammation and programmed cell death were evaluated. In addition, the renoprotective effects of Gyp XLIX were also evaluated in cisplatin- or hypoxia-treated tubular epithelial cells. The mechanisms underlying these effects were then explored using RNA sequencing.

Results: In vivo, Gyp XLIX substantially suppressed the increase in serum creatinine and blood urea nitrogen levels. Moreover, tubular damage was alleviated by Gyp XLIX as shown by periodic acid-Schiff staining, electron microscopy and molecular analysis of KIM-1. Consistently, we found that Gyp XLIX suppressed renal necroptosis though the RIPK1/RIPK3/MLKL pathway. The anti-inflammatory and antinecroptotic effects were further confirmed in vitro. Mechanistically, RNA sequencing showed that Gyp XLIX markedly suppressed the levels of IGF binding protein 7 (IGFBP7). Co-immunoprecipitation and western blot analysis further showed that Gyp XLIX reduced the binding of IGFBP7 to IGF1 receptor (IGF1R). Additionally, picropodophyllin, an inhibitor of IGF1R, abrogated the therapeutic effects of Gyp XLIX on cisplatin-induced renal cell injury; this finding indicated that Gyp XLIX may function by activating IGF1R-mediated downstream signalling Additionally, we also detected the metabolic distribution of Gyp XLIX after injection; Gyp XLIX had a high concentration in the kidney and exhibited a long retention time. These findings may shed light on the application of Gyp XLIX for AKI treatment clinically.

Conclusion: Gyp XLIX may serve as a potential therapeutic agent for AKI treatment via IGFBP7/ IGF1R-dependent mechanisms.
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http://dx.doi.org/10.1016/j.phymed.2021.153541DOI Listing
May 2021

Cross fixation bandage with long hair on scalp wound after surgery.

Indian J Dermatol Venereol Leprol 2021 Mar-Apr;87(2):306-308

Department of Dermatovenereology, West China Hospital, Sichuan University, Chengdu, China.

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http://dx.doi.org/10.25259/IJDVL_328_20DOI Listing
March 2020

Interplay of stereo-electronic, vibronic and environmental effects in tuning the chiroptical properties of an Ir(III) cyclometalated N-heterocyclic carbene.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jun 1;254:119631. Epub 2021 Mar 1.

Scuola Normale Superiore, Piazza dei Cavalieri 7, I-56126 Pisa, Italy. Electronic address:

Chiroptical spectra are among the most suitable techniques for investigating the ground and excited electronic states of chiral systems, but their interpretation is not straightforward and strongly benefits from quantum chemical simulations, provided that the employed computational model is sufficiently accurate and deals properly with stereo-electronic, vibrational averaging and environmental effects. Since the synergy among all these effects is only rarely accounted for, especially for large and flexible organometallic systems, the main aim of this contribution is to illustrate the latest developments of computational approaches rooted into the density functional theory for describing stereo-electronic effects and complemented by effective techniques to deal with vibrational modulation effects and solvatochromic shifts. In this connection, chiral iridium complexes offer an especially suitable case study in view of their bright phosphorescence, which is particularly significant for building effective light emitting diodes (OLEDs) and biomarkers and can be finely tuned by the nature of the metal ligands. For instance, a recently synthesized family of cycloiridiated complexes, KC and KD, bearing a pentahelicenic N-heterocyclic carbene (KB), has shown an enhanced long-lasting, bright phosphorescence. Deeper insights into the still unclear nature and origin of the enhancement could be gained by the interpretation of the chiroptical spectra, which is quite challenging in view of the presence of two sources of chirality, the chiral center on Ir and the chiral axis related to the helicene ligand, in addition to the relativistic effects related to the presence of the Ir center. At the same time, the large dimensions of KC and KD hamper the use of the most sophisticated (but prohibitively expensive) computational models, so that more approximate approaches must be validated on a suitable model compound. To this end, after optimizing the computational scheme on a model system devoid of the helicene moiety (KA), we have performed a comprehensive investigation of the KC and KD spectra, whose interpretation is further aided by novel graphical tools. The discussion and analysis of the results will not be focused on the theoretical background, but, rather, on practical details (specific functional, basis set, vibronic model, solvent regime) with the aim of providing general guidelines for the use of last-generation computational spectroscopy tools also by non-specialists.
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http://dx.doi.org/10.1016/j.saa.2021.119631DOI Listing
June 2021

Novel SARS2 variants identified in a Chinese girl with HUPRA syndrome.

Mol Genet Genomic Med 2021 Apr 10;9(4):e1650. Epub 2021 Mar 10.

Department of Nephrology, Children's Hospital, Chongqing Medical University, Chongqing, China.

Background: Hyperuricemia, pulmonary hypertension, renal failure, and alkaline intoxication syndrome (HUPRA syndrome) is a rare autosomal recessive mitochondrial disease. SARS2 gene encoding seryl-tRNA synthetase is the only pathogenic gene of HUPRA syndrome. All the previously reported cases with HUPRA syndrome were detected for homozygous mutation.

Methods: We identified compound heterozygous mutations causing HUPRA syndrome using whole-exome sequencing, and verifed pathogenicity with ACMG standards. All the previously published cases with SARS2 mutations were reviewed.

Results: SARS2 gene compound heterozygotes variants were detected in this Chinese patient (c.667G>A/c.1205G>A). Bioinformatics studies and protein models predict that a new variant (c.667G>A) is likely to be pathogenic. A total of six patients, five of whom were previously reported with HUPRA syndrome, were analyzed. All of the six had typical clinical manifestations of HUPRA syndrome, except the Chinese girl who had no pulmonary hypertension or alkaline intoxication. The shrunken kidney was more prominent in our proband. The average survival time for previously reported patients was 17 months, and the Chinese girl was 70 months. Three mutation variants were found, including five homozygous mutants, three of which were Palestinian (c.1169A > G), two of which were from a Spanish family (c.1205G> A), and one was a new variant (c.667G>A/c.1205G>A).

Conclusion: We found a new pathogenic form (compound heterozygous mutation) causing HUPRA syndrome, and identified a novel pathogenic site (c.667G>A) of the SARS2 gene, expanding the spectrum of SARS2 pathogenic variants. The mild phenotype in complex heterozygous mutations is described.
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http://dx.doi.org/10.1002/mgg3.1650DOI Listing
April 2021

Efficacy and Safety of CAR-T Therapy for Relapse or Refractory Multiple Myeloma: A systematic review and meta-analysis.

Int J Med Sci 2021 18;18(8):1786-1797. Epub 2021 Feb 18.

Department of Hematology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.

Multiple myeloma (MM) is incurable in spite of recent treatment improvements, highlighting the development of new therapies. Chimeric antigen receptor (CAR) T-cell therapy has dramatically changed the therapeutic effectiveness in high-risk B-cell malignancies. For relapsed/refractory multiple myeloma (RRMM), preclinical evaluations of CAR-T therapy have shown promising efficacy, thus various active clinical trials are under way. Herein, we conducted this review to summarize efficacy and safety of CAR-T therapy and provide more evidence to guide clinical treatments. We systematically searched literature based on databases (PubMed, EMBASE, Cochrane Central Register of Controlled Trials), and conference abstracts reported from American Society of Hematology (ASH), European Hematology Association (EHA) and American Society of Clinical Oncology (ASCO), in addition to other sources (www.clinicaltrials.gov, article citations). Data assessed efficacy and safety of CAR-T therapy in patients with RRMM were extracted and evaluated, and then systematically analyzed by Comprehensive Meta-analysis 3.0 (CMA 3.0). A total of 23 studies including 350 participants from different countries, diagnosed as RRMM and treated with CAR-T therapy (containing 7 antigens targeted by CARs) were combined. In summary, we discovered the pooled overall response rate (77%), complete response rate (37%) and minimal residual disease (MRD) negativity rate within responders (78%). Furthermore, the pooled relapse rate of responders was 38% and median progression-free survival was 8 months. The pooled survival rate was 87% at last follow-up (median, 12 months). In addition, the pooled grade 3-4 rates of cytokine release syndrome (CRS) and neurologic toxicities (NT) were 14% and 13%, respectively. Our study suggests that CAR-T therapy has demonstrated efficacy and safety in RRMM patients. BCMA-targeted CAR-T and anti-BCMA contained regimen have shown better efficacy.
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http://dx.doi.org/10.7150/ijms.46811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7976586PMC
February 2021

Assessing the Measurement Invariance of the Gratitude Questionnaire-5 in Chinese and American Adolescents.

Span J Psychol 2021 Mar 22;24:e17. Epub 2021 Mar 22.

University of South Carolina (US).

Given the possibility of cultural differences in the meaning and levels of gratitude among children, we evaluated the measurement invariance of the Gratitude Questionnaire-5 (GQ-5) and differences in latent means across adolescents from two distinct cultures, China and America. Data were obtained from 1,991 Chinese and 1,685 American adolescents. Confirmatory factor analysis and multigroup confirmatory factor analysis were performed to examine the factor structure and the measurement equivalence across Chinese and American adolescents. The Cronbach's alpha and Item-total Correlations of the GQ-5 were also evaluated. Results of confirmatory factor analyses provided support for the expected one-factor structure. Also, a series of multi-group confirmatory factor analyses supported full configural invariance, full metric invariance, and partial scalar invariance between the two groups. Furthermore, the findings suggested that the GQ-5 is suitable for conducting mean level comparisons. The subsequent comparison of latent means revealed that the Chinese adolescents reported significantly lower gratitude than American adolescents.
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http://dx.doi.org/10.1017/SJP.2021.19DOI Listing
March 2021

Interaction analysis of hydrochemical factors and dissolved heavy metals in the karst Caohai Wetland based on PHREEQC, cooccurrence network and redundancy analyses.

Sci Total Environ 2021 May 24;770:145361. Epub 2021 Jan 24.

Guizhou Academy of Sciences, Shanxi Road 1, Guiyang 550001, PR China.

In this study, to clarify the interaction between dissolved heavy metals and the coexisting chemical factors in karst wetland waters, surface water samples were collected from the Caohai Wetland during a water year, and the hydrochemistry and heavy metal pollution characteristics of the samples were analyzed. The main influencing factors of heavy metals in different water periods were identified through a cooccurrence network analysis. To further analyze the influence mechanism of these main influencing factors, the forms of heavy metals in the water were simulated with PHREEQC software, and the effects of these main influencing factors on the forms were analyzed by redundancy analysis. The results show that Ca was the main cation in the wetland water, while the main anion was HCO. The hydrochemical facies of the Caohai Wetland in the wet and dry seasons were Ca-Mg-SO-HCO and Ca-HCO, respectively. Cd was the main pollutant in the Caohai Wetland, with Cd levels seriously exceeding the standards. The characteristics of the karst water in the Caohai Wetland are apparent. The cooccurrence network analysis shows that pH, dissolved oxygen (DO), electrical conductivity (EC), SO and HCO are the main factors regulating heavy metals. The results of morphological simulation and analysis were used to explore the mechanism of action of these factors. These data provide geochemical information useful for water quality assessment and management plans on heavy metal pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145361DOI Listing
May 2021

Protectin DX restores Treg/T17 cell balance in rheumatoid arthritis by inhibiting NLRP3 inflammasome via miR-20a.

Cell Death Dis 2021 Mar 15;12(3):280. Epub 2021 Mar 15.

Department of Anesthesia and Critical Care, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Regulatory T-cell (Treg)/T-helper 17 (T17) cell balance plays an important role in the progression of rheumatoid arthritis (RA). Our study explored the protective effect of protectin DX (PDX), which restored Treg/T17 cell balance in RA, and the role of the nucleotide-binding domain (NOD)-like receptor protein 3 (NLRP3) inflammasome pathway in this process. Using mass spectrometry, we discovered that level of PDX decreased in active-RA patients and increased in inactive-RA patients compared with HCs, and serum PDX was a potential biomarker in RA activity detection (area under the curve [AUC] = 0.86). In addition, a collagen-induced arthritis (CIA) mice model was constructed and PDX obviously delayed RA progression in the CIA model, upregulating Tregs and anti-inflammatory cytokines while downregulating T17 cells and pro-inflammatory cytokines. Moreover, NLRP3 knockout and rescue experiments demonstrated that NLRP3 participated in PDX-mediated Treg/T17 cell balance restoration, joint injury amelioration and inflammatory-response attenuation using Nlrp3 mice. Furthermore, microarray and verified experiments confirmed that PDX reduced NLRP3 expression via miRNA-20a (miR-20a). In summary, we confirmed for the first time that PDX could effectively ameliorate CIA progression by restoring Treg/T17 cell balance, which was mediated by inhibition of the NLRP3 inflammasome pathway via miR-20a.
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http://dx.doi.org/10.1038/s41419-021-03562-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961047PMC
March 2021

Correction to Ripened Pu-erh Tea Extract Promotes Gut Microbiota Resilience against Dextran Sulfate Sodium Induced Colitis.

J Agric Food Chem 2021 Mar 11;69(11):3559. Epub 2021 Mar 11.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330031, People's Republic of China.

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http://dx.doi.org/10.1021/acs.jafc.1c01333DOI Listing
March 2021