Publications by authors named "Qin Luo"

252 Publications

The influence of polarization misalignment for modulation transfer spectrum in atom gravimeter.

Rev Sci Instrum 2021 Jul;92(7):073002

MOE Key Laboratory of Fundamental Physical Quantities Measurement and Hubei Key Laboratory of Gravitation and Quantum Physics, PGMF and School of Physics, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.

The accuracy of atom gravimeters is directly related to the Raman laser, which is used to manipulate the atomic wave packet, and the frequency of the Raman laser could be affected by temperature when the laser polarization is not along the preferred axis of the electro-optic crystal employed in the modulation transfer spectrum (MTS). This effect has been researched by modulating the laser polarization in the MTS in this work. The experimental results show that both the laser frequency and gravity measurement results have a sinusoidal dependence on temperature, and the period of the fluctuation is 0.8 °C. The systematic effect can reach 12.4 μGal when the polarization misalignment is 15°, which is a remarkable contribution to the absolute gravity measurement. The amplitude of this effect could be reduced by adjusting the laser polarization to the crystal's preferred axis. According to the result, the included angle between the laser polarization and the crystal's preferred axis should be smaller than 5° if 2 μGal accuracy is required.
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http://dx.doi.org/10.1063/5.0053340DOI Listing
July 2021

Effect of Pramipexole on Inflammatory Response in Central Nervous System of Parkinson's Disease Rat Model.

Arch Med Res 2021 Jul 1. Epub 2021 Jul 1.

Department of Neurology, Second Affiliated Hospital of Xinjiang Medical University. Electronic address:

Background: Pramipexole is the dopamine receptor agonist commonly used for treatment of PD, the effect of which on immunity played an important role in pathological process is also deserved to be further studied.

Aim Of Study: We observed the effect of pramipexole on behavior and central nervous system (CNS) inflammatory cytokines of Parkinson's Disease (PD) model rats.

Methods: We injected 3.0 μL lipopolysaccharide into the right substantia nigra compact (SNc) and ventral tegmental area (VTA) of sprague-dawley (SD) rats to establish PD models which were divided as treated group feeded with pramipexole for 14 d and untreated group feeded with saline. And SD rats were selected as control group feeded with saline. We conducted rotation test on PD model rats before and after treatment. We also performed euthanasia on all rats to obtain the striatum area and nearby tissues after treatment, measuring mRNA expression and concentration of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) by reverse transcription polymerase chain reaction test and elisa method, respectively.

Results: It was observed that the degree of behavior improvement in treated group was greater than that in untreated group. In addition, mRNA expression and concentrations of IL-6 and TNF-α in treated group were lower than those in untreated group and higher than those in control group.

Conclusions: Pramipexole improved behavior of PD model rat, and down regulated the mRNA expression and concentrations of IL-6 and TNF-α in their CNS.
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http://dx.doi.org/10.1016/j.arcmed.2021.06.007DOI Listing
July 2021

Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway.

Neoplasia 2021 Jul 18;23(7):692-703. Epub 2021 Jun 18.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, PR China.

Cancer-associated fibroblasts cells (CAFs) confer a rapid growth and metastasis ability of endometrial cancer (EC) via exosomes-mediated cellular communication. Long non-coding RNA nuclear enriched abundant transcript 1 (lncRNA NEAT1) drives the malignant phenotypes of EC cells. However, the role of exosomal NEAT1 from CAFs in EC progression remains ambiguous, which needs to be investigated. In our study, NEAT1 and YKL-40 were up-regulated, while miR-26a/b-5p was down-regulated in EC tissues. Moreover, NEAT1 expression was increased in CAF-exosomes compared with that in NF-exosomes. In addition, the exosomal NEAT1 derived from CAFs could transfer to EC cells and promote YKL-40 expression. Further exploration showed that exosomal NEAT1 enhanced YKL-40 expression via regulating miR-26a/b-5p-STAT3 axis in EC cells. More importantly, exosomal NEAT1 accelerated in vivo tumor growth via miR-26a/b-5p-STAT3-YKL-40 axis. Taken together, our study reveals that exosomal NEAT1 from CAFs contributes to EC progression via miR-26a/b-5p-mediated STAT3/YKL-40 pathway, which indicates the therapeutic potential of exosomal NEAT1 for treating EC.
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http://dx.doi.org/10.1016/j.neo.2021.05.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233173PMC
July 2021

Combined Treatment of Cinobufotalin and Gefitinib Exhibits Potent Efficacy against Lung Cancer.

Evid Based Complement Alternat Med 2021 20;2021:6612365. Epub 2021 Mar 20.

Department of Respiratory Neurology, The Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830000, China.

This study aimed to evaluate the efficacy of cinobufotalin combined with gefitinib in the treatment of lung cancer. A549 cells were treated with gefitinib, cinobufotalin, or cinobufotalin plus gefitinib. MTT assay, annexin-V/PI staining and flow cytometry, TUNEL staining, DCFH-DA staining, Western blot, and real-time RT-PCR were performed to investigate the synergistic inhibitory effect of cinobufotalin combined with gefitinib on the growth of A549 cells. Results showed that cinobufotalin synergized with gefitinib displayed inhibited cell viability and enhanced apoptosis in the combination group. Cinobufotalin combined with gefitinib induced a significant enhancement in reactive oxygen species (ROS) production accompanied by cell cycle arrest in the S phase arrest, characterized by upregulation of p21 and downregulation of cyclin A, cyclin E, and CDK2. Besides, cinobufotalin plus gefitinib downregulated the levels of HGF and c-Met. In summary, cinobufotalin combined with gefitinib impedes viability and facilitates apoptosis of A549 cells, indicating that the combined therapy might be a new promising treatment for lung cancer patients who are resistant to gefitinib.
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http://dx.doi.org/10.1155/2021/6612365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189783PMC
March 2021

The benefit of exercise-based rehabilitation programs in patients with pulmonary hypertension: a systematic review and meta-analysis of randomized controlled trials.

Pulm Circ 2021 Apr-Jun;11(2):20458940211007810. Epub 2021 May 27.

Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Several studies have suggested that exercise capacity and quality of life are reduced in patients with pulmonary hypertension, and exercise-based rehabilitation can improve exercise capacity and quality of life in patients with pulmonary hypertension. The aim of this study is to assess the efficacy and safety of exercise-based rehabilitation in patients with pulmonary hypertension through a meta-analysis of randomized controlled trials. We searched PubMed, Embase, Medline, and the Cochrane Central Register of Controlled Trials up to November 2018. All randomized controlled trials comparing exercise capacity and quality of life between patients undergoing exercise-based rehabilitation and those undergoing non-exercise training were included. Data were extracted separately and independently by two investigators, and discrepancies were arbitrated by the third investigator. We used the random-effects model to analyze the results, the GRADE to assess the risk of bias in the included studies, and I statistic to estimate the degree of heterogeneity. Nine randomized controlled trials are included; however, only seven randomized controlled trials were able to extract data. Including inpatients and outpatients, the total number of participants was 234, most of whom were diagnosed as pulmonary artery hypertension. The study duration ranged from 3 to 15 weeks. The mean six-minute walking distance after exercise training was 51.94 m higher than control (27.65-76.23 m,  = 234, 7 randomized controlled trials, low quality evidence), the mean peak oxygen uptake was 2.96 ml/kg/min higher (2.49-3.43 ml/kg/min,  = 179, 4 randomized controlled trials, low-quality evidence) than in the control group. In conclusion, our finding suggests that an exercise-based training program positively influences exercise capacity in patients with pulmonary hypertension.
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http://dx.doi.org/10.1177/20458940211007810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165522PMC
May 2021

Exosomal S100A4 derived from highly metastatic hepatocellular carcinoma cells promotes metastasis by activating STAT3.

Signal Transduct Target Ther 2021 May 26;6(1):187. Epub 2021 May 26.

Department of General Surgery, Huashan Hospital, Cancer Metastasis Institute, Fudan University, Shanghai, China.

Intercellular cross-talk plays important roles in cancer progression and metastasis. Yet how these cancer cells interact with each other is still largely unknown. Exosomes released by tumor cells have been proved to be effective cell-to-cell signal mediators. We explored the functional roles of exosomes in metastasis and the potential prognostic values for hepatocellular carcinoma (HCC). Exosomes were extracted from HCC cells of different metastatic potentials. The metastatic effects of exosomes derived from highly metastatic HCC cells (HMH) were evaluated both in vitro and in vivo. Exosomal proteins were identified with iTRAQ mass spectrum and verified in cell lines, xenograft tumor samples, and functional analyses. Exosomes released by HMH significantly enhanced the in vitro invasion and in vivo metastasis of low metastatic HCC cells (LMH). S100 calcium-binding protein A4 (S100A4) was identified as a functional factor in exosomes derived from HMH. S100A4 exosomes significantly promoted tumor metastasis both in vitro and in vivo compared with S100A4 exosomes or controls. Moreover, exosomal S100A4 could induce expression of osteopontin (OPN), along with other tumor metastasis/stemness-related genes. Exosomal S100A4 activated OPN transcription via STAT3 phosphorylation. HCC patients with high exosomal S100A4 in plasma also had a poorer prognosis. In conclusion, exosomes from HMH could promote the metastatic potential of LMH, and exosomal S100A4 is a key enhancer for HCC metastasis, activating STAT3 phosphorylation and up-regulating OPN expression. This suggested exosomal S100A4 to be a novel prognostic marker and therapeutic target for HCC metastasis.
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http://dx.doi.org/10.1038/s41392-021-00579-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149717PMC
May 2021

Development and validation of a novel diagnostic nomogram model to predict primary aldosteronism in patients with hypertension.

Endocrine 2021 Sep 24;73(3):682-692. Epub 2021 May 24.

Hypertension Center of People's Hospital of Xinjiang Uygur Autonomous Region; Xinjiang Hypertension Institute, National Health Committee Key Laboratory of Hypertension Clinical Research, No. 91 Tianchi Road Urumqi, 830001, Xinjiang, China.

Purpose: Primary aldosteronism (PA) remains, to a large extent, an under-diagnosed disease. We aimed to develop and validate a novel clinical nomogram to predict PA based on routine biochemical variables including new ones, calcium-phosphorus product.

Methods: Records from 806 patients with hypertension were randomly divided into 70% (n = 564) as the training set and the remaining 30% (n = 242) as the validation set. Predictors for PA were extracted to construct a nomogram model based on regression analysis of the training set. An internal validation was performed to assess the nomogram model's discrimination and consistency using the area under the curve for receiver operating characteristic curves and calibration plots. The diagnostic accuracy was compared between nomogram and other known prediction models, using receiver operating characteristics (ROC) and decision curve analyses (DCA).

Results: Female gender, serum potassium, serum calcium-phosphorus product, and urine pH were adopted as predictors in the nomogram. The nomogram resulted in an area under the curve of 0.73 (95% confidence interval: 0.68-0.78) in the training set and an area under the curve of 0.68 (0.59-0.75) in the validation set. Predicted probability and actual probability matched well in the nomogram (p > 0.05). Based on ROC and DCA, 21-70% threshold to predict PA in the nomogram model was clinically useful.

Conclusions: We have developed a novel nomogram to predict PA in hypertensive individuals based on routine biochemical variables. External validation is needed to further demonstrate its predictive ability in primary care settings.
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http://dx.doi.org/10.1007/s12020-021-02745-7DOI Listing
September 2021

Absence of Toll-like receptor 7 protects mice against Pseudomonas aeruginosa pneumonia.

Int Immunopharmacol 2021 Jul 10;96:107739. Epub 2021 May 10.

Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. Electronic address:

Toll-like receptor 7 (TLR7) is a sensor of microbial ssRNA that participates in the immune response process in many diseases. We herein sought to establish the role of TLR7 in Pseudomonas aeruginosa pneumonia. Pneumonia model was created by intratracheally injecting Pseudomonas aeruginosa and the effects of TLR7 on survival, bacterial burden, lung pathology, cytokine and chemokine production, and pulmonary leukocyte recruitment were measured after Pseudomonas aeruginosa challenge. TLR7 expression was significantly elevated in WT mice after Pseudomonas aeruginosa infection. TLR7 mice demonstrated enhanced survival, bacterial clearance, leukocyte infiltration, and macrophages phagocytic activity, and decreased pathology and capillary leakage. Besides, improved survival and bacterial clearance were observed in WT mice treated with TLR7 antagonist IRS661. More importantly, lack of TLR7 suppressed pro-inflammatory cytokine production and induced anti-inflammatory cytokine production in mice lungs. Finally, neutralized IL-10 damaged the bacterial clearance ability of TLR7 deficient mice, leading to decreased survival. Collectively, absence of TLR7 provided protective effects during Pseudomonas aeruginosa pneumonia and suggested that TLR7 could act as a novel immune target to treat clinical cases with Pseudomonas aeruginosa pneumonia.
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http://dx.doi.org/10.1016/j.intimp.2021.107739DOI Listing
July 2021

Supplementation with Iron in Pulmonary Arterial Hypertension. Two Randomized Crossover Trials.

Ann Am Thorac Soc 2021 06;18(6):981-988

National Heart and Lung Institute and.

Iron deficiency, in the absence of anemia, is common in patients with idiopathic and heritable pulmonary arterial hypertension (PAH) and is associated with a worse clinical outcome. Oral iron absorption may be impeded by elevated circulating hepcidin concentrations. The safety and benefit of parenteral iron replacement in this patient population is unclear. To evaluate the safety and efficacy of parenteral iron replacement in PAH. In two randomized, double-blind, placebo-controlled 12-week crossover studies, 39 patients in Europe received a single infusion of ferric carboxymaltose (Ferinject) (1,000 mg or 15 mg/kg if weight <66.7 kg) or saline as placebo, and 17 patients in China received iron dextran (Cosmofer) (20 mg iron/kg body weight) or saline placebo. All patients had idiopathic or heritable PAH and iron deficiency at entry as defined by a serum ferritin <37 μg/L or iron <10.3 μmol/L or transferrin saturations <16.4%. Both iron treatments were well tolerated and improved iron status. Analyzed separately and combined, there was no effect on any measure of exercise capacity (using cardiopulmonary exercise testing or 6-minute walk test) or cardiopulmonary hemodynamics, as assessed by right heart catheterization, cardiac magnetic resonance, or plasma NT-proBNP (N-terminal-pro hormone brain natriuretic peptide) at 12 weeks. Iron repletion by administration of a slow-release iron preparation as a single infusion to patients with PAH with iron deficiency without overt anemia was well tolerated but provided no significant clinical benefit at 12 weeks. Clinical trial registered with ClinicalTrials.gov (NCT01447628).
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http://dx.doi.org/10.1513/AnnalsATS.202009-1131OCDOI Listing
June 2021

mtROS-mediated Akt/AMPK/mTOR pathway was involved in Copper-induced autophagy and it attenuates Copper-induced apoptosis in RAW264.7 mouse monocytes.

Redox Biol 2021 05 1;41:101912. Epub 2021 Mar 1.

Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agriculture University, Wenjiang, Chengdu, 611130, China. Electronic address:

Copper (Cu) is a trace element necessary in animals as well as human beings. However, excessive Cu is toxic to immunocytes, but the precise mechanism is largely unclear so far. This work was conducted aiming to examine the Cu-mediated autophagy mechanism together with its role in Cu toxicology in RAW264.7 cells. Here, we demonstrated that CuSO reduced the cell viability depending on its dose. CuSO could obviously increase autophagy in RAW264.7 cells. According to the obtained results, CuSO induced autophagy through Akt/AMPK/mTOR pathway which characterized by down regulation of p-Akt (Ser473)/Akt, p-mTOR/mTOR, p-ULK1(Ser757)/ULK1 and subsequent up-regulation of p-AMPKα/AMPKα and p-ULK1(Ser555)/ULK1. Furthermore, CuSO significantly induced the production of mitochondrial reactive oxygen species (mtROS). In addition, CuSO-mediated apoptosis and autophagy might be suppressed through suppressing mtROS generation by exposure to Mito-TEMPO. Intriguingly, autophagy promotion with rapamycin could decrease the apoptosis and the inhibition of autophagy with knock down Atg5 could enhance the apoptosis induced by CuSO. Moreover, our results suggested that mtROS is the original cause in CuSO-induced apoptosis and autophagy. Additionally, CuSO induced autophagy through mtROS-dependent Akt/AMPK/mTOR signalling pathwayin RAW264.7 cells. Moreover, autophagy activation might potentially generate a protection mechanism for improving CuSO-induced RAW264.7 cell apoptosis.
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http://dx.doi.org/10.1016/j.redox.2021.101912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944049PMC
May 2021

Minor Change of Plasma Renin Activity during the Saline Infusion Test Provide an Auxiliary Diagnostic Value for Primary Aldosteronism.

Int J Endocrinol 2021 17;2021:5757305. Epub 2021 Feb 17.

Hypertension Center of People's Hospital of Xinjiang Uygur Autonomous Region, Xinjiang Hypertension Institute, National Health Committee Key Laboratory of Hypertension Clinical Research, Urumqi, China.

Objective: To clarify whether it has some hidden diagnostic values for PA, especially in the case of an inconclusive SIT result, we investigated the difference in changes of plasma renin activity (PRA) during SIT between patients with PA and non-PA.

Methods: We measured and compared the SIT parameters of 159 PA patients, 368 non-PA patients, and 43 inconclusive patients who were included in this study.

Results: The PA group showed a minor change of PRA during the SIT (ΔPRA, defined as (pre-SIT PRA-post-SIT PRA)) compared with the non-PA group (0.17 ng/ml/h vs. 1.07 ng/ml/h, < 0.001). According to ROC analysis, ΔPRA showed a greater AUC than post-SIT PRA (0.897 vs. 0.855, < 0.001). The cutoff value was 0.5 ng/ml/h, with 90.3% sensitivity and 78.6% specificity. When combined with ARR post-SIT, it showed 81.6% sensitivity and 97.0% specificity for PA diagnosis. Further analysis of 43 patients with an inconclusive SIT result who completed AVS found that ΔPRA was smaller in the confirmed PA group compared with the unconfirmed PA group (0.19 ng/ml/h vs. 0.29 ng/ml/h, < 0.05); there was no significant difference in PAC post-SIT between two groups. ΔPRA ≤ 0.21 ng/ml/h provides 71.4% sensitivity, 80.0% specificity, and 87.0% PPV for their PA diagnosis.

Conclusions: PA patients show minor PRA change during SIT; the change of PRA during SIT provides an auxiliary diagnostic value for PA, especially in patients with an inconclusive SIT result.
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http://dx.doi.org/10.1155/2021/5757305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904345PMC
February 2021

The value of cardiopulmonary exercise testing in the diagnosis of pulmonary hypertension.

J Thorac Dis 2021 Jan;13(1):178-188

Center for Pulmonary Vascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Cardiopulmonary exercise testing (CPET) continuously analyzes the gas exchange of patients during rest, exercise, recovery, and simultaneously records the response of the cardiopulmonary system. This study aimed to observe the characteristics of CPET in patients with pulmonary hypertension (PH) and to explore the cutoff value of CPET variables in detecting PH. The diagnostic value of CPET was also investigated in a subgroup of patients who had an incorrect or missed diagnosis of PH by echocardiography.

Methods: Treatment-naïve patients with suspected PH who were admitted to Fuwai Hospital from January 2017 to August 2018 were consecutively enrolled. The gold standard criterion for PH was defined as mean pulmonary artery pressure (mPAP) ≥25 mmHg at rest, measured by right heart catheterization. General clinical materials, echocardiography, hemodynamics, and CPET data of the patients were collected and compared between groups. Logistic regression analyses were performed to explore the CPET variables that were independently associated with PH. To further validate the value of CPET for diagnosing PH, the CPET cutoff values obtained from receiver operating characteristic (ROC) curve analysis were used in patients who had an incorrect or missed diagnosis by echocardiography.

Results: Five hundred and fifty-nine patients were included in the study. Among them, patients with PH had significantly poorer CPET variables. Multivariate logistic regression analysis showed that peak work rate (WR), peak oxygen uptake (VO), and end-tidal carbon dioxide partial pressure (PetCO) at the anaerobic threshold (AT) were independently associated with PH after adjustment for age, sex, and body mass index. The above three CPET variables were all negatively correlated with mPAP. The combined CPET variable including peak WR, peak VO and PetCO at AT had the largest area under the ROC curve for the diagnosis of PH (0.890, 95% CI: 0.852-0.927, P<0.001). The cutoff value was 0.86, and the sensitivity and specificity were 81.8% and 86.5%, respectively. Using this cutoff value, 83.7% of patients who were misdiagnosed and 67.9% of patients who had a missed diagnosis by echocardiography were identified.

Conclusions: PH patients have decreased cardiopulmonary reserve, lower exercise tolerance, and increased ineffective ventilation. The combination of peak WR, peakVO, and PetCO at AT had increased sensitivity and specificity for the diagnosis of PH, and increased the specificity for identifying patients who had been misdiagnosed as PH by echocardiography.
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http://dx.doi.org/10.21037/jtd-20-1061bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867820PMC
January 2021

Ultrasound cycloplasty in Chinese glaucoma patients: Results of a 6-month prospective clinical study.

Ophthalmic Res 2021 Feb 4. Epub 2021 Feb 4.

Purpose: The aim of this study was to evaluate the short-term efficacy and safety of ultrasound cycloplasty (UCP) procedure in Chinese glaucoma patients.

Methods: As a single-centre, prospective, non-comparative study, 23 eyes of 23 patients suffering from glaucoma with uncontrolled intraocular pressure (IOP) ≥ 21 mmHg underwent a multi-dose UCP treatment with the activations of 6, 8 or10 sectors. Types of glaucoma include primary open-angle glaucoma (POAG) (7/23), primary angle closure glaucoma (PACG) (9/23) and secondary glaucoma (SG) (7/23). A complete ophthalmic examination including intraocular pressure (IOP) measurements was performed before UCP procedure and at 1 day, 1 month, 3months and 6 months after the procedure. An IOP reduction of ≥20% and IOP > 5 mmHg without increasing hypotensive medication at the follow-up visit was defined as therapeutic success. The post-operative complications were also recorded and compared to baseline for safety evaluation.

Results: The mean baseline IOP of 23 treated eyes was 37.2 ± 12.1 mmHg. The IOP reduction after UCP procedure were 23%, 49%, 33% and 34% at 1 day, 1 month, 3 months and 6 months, respectively. Thus, the corresponding overall therapeutic success rates reached 61% (14/23), 83% (19/23), 65% (15/23) and 61% (14/23), respectively. Baseline IOPs of 8 and 10 sectors group (37.0 ± 9.9 mmHg and 50.1 ± 12.2 mmHg) were significantly higher than that of 6 sectors group (30.1 ± 8.2 mmHg). Therapeutic success rates of 6, 8 and 10 sectors groups reached 44% (4/9), 56% (5/9) and 100% (5/5), respectively. There were the highest percentage of IOP reduction (50% and 41%) and therapeutic success rate (6/7; 86% and 7/9; 78%) in SG group and PACG groups respectively. In addition, pre-operative ocular pain symptoms of four patients were all disappeared within one week after UCP. No serious intra-operative or post-operative complications occurred.

Conclusion: UCP procedure is an effective and well-tolerated treatment to reduce IOP in Chinese glaucoma patients, which offered a novel alternative for glaucoma treatment.
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http://dx.doi.org/10.1159/000515013DOI Listing
February 2021

TLR7 Expression Aggravates Invasive Pulmonary Aspergillosis by Suppressing Anti- Immunity of Macrophages.

Infect Immun 2021 Mar 17;89(4). Epub 2021 Mar 17.

Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China

Toll-like receptors (TLRs) play a critical role in early immune recognition of , which can regulate host defense during invasive pulmonary (IPA). However, the role of TLR7 in the pathogenesis of IPA remains unknown. In this study, an model of IPA was established to investigate the contribution of TLR7 to host anti- immunity upon invasive pulmonary infection. The effects of TLR7 on phagocytosis and killing capacities of by macrophages and neutrophils were investigated We found that TLR7 knockout mice exhibited lower lung inflammatory response and tissue injury, higher fungal clearance, and greater survival in an model of IPA compared with wild-type mice. TLR7 activation by R837 ligand led to wild-type mice being more susceptible to invasive pulmonary infection. Macrophages, but not neutrophils, were required for the protection against IPA observed in TLR7 knockout mice. Mechanistically, TLR7 impaired phagocytosis and killing of by macrophages but not neutrophils. Together, these data identify TLR7 as an important negative regulator of anti- innate immunity in IPA, and we propose that targeting TLR7 will be beneficial in the treatment of IPA.
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http://dx.doi.org/10.1128/IAI.00019-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8090942PMC
March 2021

Effect of spironolactone on cardiovascular morbidity and mortality in patients with hypertension and glucose metabolism disorders (ESCAM): a study protocol for a pragmatic randomised controlled trial.

BMJ Open 2020 11 19;10(11):e038694. Epub 2020 Nov 19.

Hypertension Center of People's Hospital of Xinjiang Uygur Autonomous Region, Xinjiang Hypertension Institute, National Health Committee Key Laboratory of Hypertension Clinical Research, Urumqi, China.

Introduction: Hypertension combined with diabetes and hypokalemia is more likely to develop hyperaldosteronism and is at higher risk of cardiovascular events. There is evidence that activation of aldosterone and mineralocorticoid receptors may play a significant role in the occurrence of cardiovascular events in patients with hypertension and diabetes. Clinical studies have demonstrated that spironolactone can reduce the incidence of cardiovascular events in patients with chronic kidney diseases or severe heart failure. However, the effect of spironolactone on cardiovascular risk in patients with hypertension and glucose metabolism disorders (GMD) and low potassium has been scarcely studied. Therefore, this study aims to evaluate whether add-on spironolactone (conventional antihypertensive drugs alone vs conventional antihypertensive drugs+spironolactone) can reduce the morbidity and mortality of cardiovascular events in this population.

Methods And Analysis: In this multicentre, randomised, parallel-controlled study, a total of 7140 hypertensive patients aged 45-75 years with GMD and low potassium will be randomised in a 1:1 manner to the control or the spironolactone group (20 mg/day or with a maximum dose of 40 mg). The primary objective is to estimate the difference in the HR of composite cardiovascular events between the two groups. We will also assess the effects of spironolactone on individual cardiovascular events and the progression of diabetes and renal dysfunction.

Ethics And Dissemination: This protocol was approved by the Independent Ethics Committee of People's Hospital of Xinjiang Uygur Autonomous Region (no. 2020020618). The results will be disseminated in peer-reviewed journals and at scientific conferences.

Trial Registration Number: ChiCTR2000028909.
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http://dx.doi.org/10.1136/bmjopen-2020-038694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678363PMC
November 2020

L51P, a novel mutation in the PAS domain of hERG channel, confers long QT syndrome by impairing channel activation.

Am J Transl Res 2020 15;12(12):8040-8049. Epub 2020 Dec 15.

Department of Cardiovascular Medicine, The Second Xiangya Hospital, Central South University Changsha, Hunan, China.

The (hERG) potassium channel mediates the repolarization of ventricular action potentials. Mutations in the cause long QT syndrome (LQTS) and are associated with cardiac arrhythmias and sudden death. Here, we functionally analyzed a mutation of hERG potassium channel (p.L51P), gaining novel insights into clinical genotype-phenotype relationships. Potassium currents were recorded by whole-cell patch clamping in HEK293 cells transiently transfected with wild-type and/or mutant hERG potassium channel. Immunofluorescence assay and confocal imaging were undertaken to study the effects of L51P mutation on channel trafficking. The models of the protein structure of hERG and its mutations are predicted by Amber16 software. Molecular dynamics (MD) of individual protein were performed with Particle Mesh Ewald (PME). The production of MD simulations of hERG-WT and hERG-Mut at constant pressure and temperature were carried out with SHAKE. L51 was a conservative amino acid, located in the Per-Arnt-Sim (PAS) domain of the amino terminus. L51P caused loss of function via impairing channel activation. L51P was predicted to destroy hydrophobic structure in the PAS domain, thus causing the failure of channel opening. In summary, the present study identifies L51P as a novel mutation of hERG potassium channel. L51P mutation mechanistically impairs channel activation, reducing channel functionality.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791479PMC
December 2020

Numerical investigation on image degradation caused by aero-optical effects of the supersonic mixing layer.

Appl Opt 2020 Dec;59(35):11059-11069

The supersonic mixing layer over the optic window of a high-speed imaging guided vehicle is the primary turbulent flow causing aero-optical effects such as the target image blurring, jittering, and offsetting. Employing the large eddy simulation and ray tracing methods, this paper presents a numerical investigation on target image degradation of a beam passing through the supersonic mixing layer under different conditions. The distorted wavefront, degraded target image, and peak signal-to-noise ratio of the degraded image are proposed to quantitatively evaluate the target image degradation. On the whole, the degraded target image is mainly shown as image blurring, and the larger the convective Mach number of the flow field, the more severe the image degradation. It is found that the beam incident position should be away from transition area of the supersonic mixing layer for a clearer image, the larger inlet fluid density difference results in a more severe target image degradation due to the stronger density fluctuation of turbulent flow structures, and the optimal beam incident angle is about 30° for the current calculation conditions.
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http://dx.doi.org/10.1364/AO.409548DOI Listing
December 2020

Bioactive dammarane triterpenoid saponins from the leaves of Cyclocarya paliurus.

Phytochemistry 2021 Mar 22;183:112618. Epub 2020 Dec 22.

College of Pharmacy, Guilin Medical University, Guilin, 541199, People's Republic of China. Electronic address:

Thirteen undescribed dammarane triterpenoid saponins (cypaliurusides A-M), including eleven seco-dammarane type triterpenoids, were isolated from Cyclocarya paliurus. Each of these compounds has the unique feature of having a monosaccharide attached to C-11, rather than C-12, compared to the same type of saponins found in this plant. The structures of them were determined by comprehensive analysis of 1D, 2D NMR and HRESIMS data. Cypaliuruside J showed significant α-glucosidase inhibitory effect with IC value of 2.22 ± 0.13 μM. In addition, Cypaliurusides F and K exhibited modest cytotoxic activities against selected human cancer cell lines in vitro, with IC values ranging from 4.61 ± 0.13 to 15.23 ± 3.88 μM.
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http://dx.doi.org/10.1016/j.phytochem.2020.112618DOI Listing
March 2021

Nuclear magnetic resonance reveals postprandial low-density lipoprotein cholesterol determined by enzymatic method could be a misleading indicator.

Clin Chim Acta 2021 Mar 15;514:59-65. Epub 2020 Dec 15.

Department of Cardiovascular Medicine, the Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China; Research Institute of Blood Lipid and Atherosclerosis, Changsha, Hunan 410011, China. Electronic address:

Background: Serum concentration of low-density lipoprotein cholesterol (LDL-C) is markedly reduced after a meal. Does postprandial cholesterol in LDL truly decline via clearance of LDL particles or is there simply a redistribution of cholesterol in LDL subclasses? Thus, we sought to evaluate whether postprandial decline of LDL-C reflects a reduction of LDL particle and to assess the correlation between proprotein convertase subtilisin/kexin type 9 (PCSK9) concentration and postprandial atherogenic lipoproteins profile.

Methods: Eighty-seven persons were enrolled in this study. We measured lipid profiles by enzymatic and nuclear magnetic resonance (NMR)-based methods and serum PCSK9 concentration by enzyme-linked immunosorbent assays before and after a meal. Plasma samples were collected after a 10-h fasting and 2 and 4 h post-meal.

Results: Compared to the fasting status, there was significant postprandial decline of LDL-C measured enzymatically (LDL-Ce) at 2nd and 4th h [99.38 (80.43, 120.65) vs 95.51 (74.25, 117.17) vs 87.01 (69.99, 108.28) mg/dl, p < 0.000]. But there was no significant reduction in LDL particle and its cholesterol content (LDL-Cn) determined by NMR. Just the postprandial large LDL particle [186.45 (151.36, 229.42) vs 176.92 (147.43, 220.91) vs 181.77 (149.05, 224.17), p < 0.000] and its cholesterol content [19.10 (15.09, 22.37) vs 18.28 (14.59, 21.84) vs 17.79 (14.62, 22.14), p < 0.000] were greatly decreased at 2nd and 4th h compared to the fasting one. Interestingly, postprandial serum PCSK9 was decreased at 2nd and 4th h compared with fasting concentration [298.75 (233.25, 396.92) vs 257.34 (207.52, 342.36) vs 250.57 (215.02, 339.66) ng/ml, p < 0.000]. The postprandial percent decrease in serum PCSK9 at 4th h was positively correlated to the percent decline in postprandial LDL-Ce (r = 0.252, p = 0.019) but was independently associated with the percent increase in remnant cholesterol (r = 0.262, p = 0.016).

Conclusions: Postprandial decline of LDL-C determined enzymatically was not confirmed by NMR-based methods. Indeed, there exists cholesterol redistribution in LDL subclasses following a meal. The decrease of postprandial PCSK9 may be secondary to the increase in intrahepatic lipids following food intake.
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http://dx.doi.org/10.1016/j.cca.2020.12.013DOI Listing
March 2021

A Dendrite-Free Lithium/Carbon Nanotube Hybrid for Lithium-Metal Batteries.

Adv Mater 2021 Jan 13;33(4):e2006702. Epub 2020 Dec 13.

School of Materials Science and Engineering, Jiangxi University of Science and Technology (JXUST), Ganzhou, 341000, P. R. China.

Lithium (Li) metal is promising in the next-generation energy storage systems. However, its practical application is still hindered by the poor cycling performance and serious safety issues for the consequence of dendritic Li. Herein, a dendrite-free Li/carbon nanotube (CNT) hybrid is proposed, which is fabricated by direct coating molten Li on CNTs, for Li-metal batteries. The favorable thermodynamic and kinetic conditions are the powerful force to drive the rapid lift upwards and infusion of molten Li into CNTs network, which is the key to form a uniform metallic layer in Li/CNTs hybrid. The obtained hybrid indicates super-stable functions even at an ultrahigh current density of 40 mA cm for 2000 cycles with a stripping/plating capacity of 2 mAh cm in symmetric cells. Subsequently, this hybrid also demonstrates a significantly decreased resistance, excellent cycling stability at high current density and flexibility in the full Li-S battery. This work provides valuable concepts in fabricating Li anodes toward Li-metal batteries and beyond for their high-level services.
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http://dx.doi.org/10.1002/adma.202006702DOI Listing
January 2021

Long-term impact of spironolactone compliance on microalbuminuria in patients with primary aldosteronism.

Hypertens Res 2021 04 10;44(4):426-434. Epub 2020 Dec 10.

Hypertension Center of People's Hospital of Xinjiang Uygur Autonomous Region; Xinjiang Hypertension Institute, National Health Committee Key Laboratory of Hypertension Clinical Research, Urumqi, 830001, Xinjiang, China.

Patients with primary aldosteronism (PA) have a high prevalence of microalbuminuria (MAU), which leads to more severe systemic vascular damage. However, the primary recommended drug treatment for PA, spironolactone (SPL), has had poor patient compliance owing to its adverse effects, and the effect of SPL compliance on MAU has not been fully evaluated in patients with PA. We analyzed the effect of SPL compliance on endothelial dysfunction by assessing MAU in patients with PA. The study included 145 confirmed PA patients who received long-term medical treatment (mean, 5 years). As expected, compliance with SPL treatment improved patients' blood pressure and serum potassium levels. Patients with PA who complied fully with SPL treatment had a lower rate of MAU than noncompliant patients (13.73% versus 34.88%, respectively; P = 0.004). Multivariate logistic regression analyses adjusted for age and sex showed that continuous SPL treatment was associated with a lower presence of MAU (odds ratio, 0.319; 95% confidence interval, 0.135-0.750; P = 0.009). This association remained significant after further adjusting for other major risk factors. However, in the subgroup analysis, the protective effect against MAU was limited in compliant patients treated with ≥40 mg/day SPL compared with noncompliant patients (9.62% versus 34.88%, respectively; P < 0.05). Our findings demonstrated that in addition to improving high blood pressure and hypokalemia, full compliance with the appropriate dose of SPL may benefit endothelial function, as reflected by a lower prevalence of MAU in patients with PA.
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http://dx.doi.org/10.1038/s41440-020-00589-8DOI Listing
April 2021

Principal component analysis of routine blood test results with Parkinson's disease: A case-control study.

Exp Gerontol 2021 02 6;144:111188. Epub 2020 Dec 6.

Department of Neurology, Second Affiliated Hospital of Xinjiang Medical University, No. 38, Nanhudonglu Road, Urumqi 830054, China. Electronic address:

This study aimed to explore the association of routine blood test values and blood cell ratios with the risk or severity of Parkinson's disease (PD). The medical records of 453 PD patients and 436 controls were retrospectively reviewed. The severity of PD was quantified by the modified Hoehn-Yahr (HY) scale. We performed principal component analysis (PCA) of significant values/ratios and used logistic regression analysis to explore the relationship between principal components (PCs) and the risk of PD. Spearman correlation and ordinal logistic regression analyses were performed to explore the relationship between indicators and the severity of PD. The PCA generated 9 PCs, which contributed to 90.86% of the total variance. Logistic regression analysis revealed positive associations of PC2 (a measure monocyte ratios) and PC6 (a measure of platelet ratios and volume) and negative associations of PC1 (a comprehensive measure of lymphocyte, eosinophil, neutrophil, and red blood cell values), PC4 (a measure of red blood cell values), and PC7 (a measure of red blood cell values and platelet volume) with the risk of PD. However, we observed no associations of variables with the severity of PD. In conclusion, PCA reduced the dimensionality of the data. Peripheral blood disorders may be associated with PD.
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http://dx.doi.org/10.1016/j.exger.2020.111188DOI Listing
February 2021

Tirofiban combined with heparin's effect and safety in the treatment of mild to moderate acute ischemic stroke.

Neurol Res 2021 Mar 4;43(3):220-224. Epub 2020 Dec 4.

Department of Neurology, Zigong First People's Hospital , Sichuan, China.

Tirofiban can be used to treat patients with acute ischemic stroke (AIS), this study was to evaluate the efficacy and safety of tirofiban combined with heparin in the treatment of mild to moderate AIS. A total of 98 patients with mild to moderate AIS randomly were divided into 2 groups within 48 h: the treatment group treated with tirofiban and, and the control group treated with aspirin + clopidogrel. The treatment group was given the same scheme as the control group after the treatment of tirofiban combined with heparin for 48 h. It was found that, compared with the control group, a significant decreased National Institute of Health stroke scale (NIHSS) was found in 48 h and 14 d, especially to the Barthel index (BI) in the treatment group (P < 0.05). Furthermore, Modified Rankin Scale (MRS, ≤2) in the treatment group was significantly upregulated in 90 d (P < 0.05). However, there were no significant differences in the adverse drug reactions between the two groups. It was indicated that nerve function and long-term prognosis in patients undergoing heparin for mild to moderate AIS were obviously improved than the control group.
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http://dx.doi.org/10.1080/01616412.2020.1839690DOI Listing
March 2021

MiR-142-5p Suppresses Lung Cancer Cell Metastasis by Targeting Yin Yang 1 to Regulate Epithelial-Mesenchymal Transition.

Cell Reprogram 2020 Dec;22(6):328-336

General Department (Area1), the Third Affiliated Hospital of Xinjiang Medical University, Urumqi, China.

This study aimed to investigate the mechanism of miR-142-5p and Yin Yang 1 (YY1) on regulating epithelial-mesenchymal transition (EMT) in lung cancer cell metastasis. The expressions of YY1 and miR-142-5p in different lung cancer cell lines were negatively correlated. The results of the dual-luciferase reporter assay further validated that miR-142-5p directly targeted YY1. Subsequently, transwell assays, wound-healing assay, and transplantation tumor model in nude mice proved that YY1 could promote the metastasis of lung cancer cells, whereas miR-142-5p impaired the stimulating effect of YY1 on the metastasis ability of lung cancer cells and . Western blot and quantitative real-time polymerase chain reaction analysis of the EMT-related proteins indicated that YY1 could enhance the metastasis ability of lung cancer cells by promoting EMT. On the contrary, miR-142-5p constrained the expression of mesenchymal markers by targeting YY1, reversed the differentiation of cells into mesenchymal cells, and weakened the metastasis ability of tumor cells and . In summary, miR-142-5p may regulate the expressions of EMT-related proteins by targeting YY1, thereby inhibiting lung cancer metastasis, which provides a promising therapeutic target for lung cancer.
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http://dx.doi.org/10.1089/cell.2020.0023DOI Listing
December 2020

High-Flow Nasal Cannula for Chronic Obstructive Pulmonary Disease with Acute Compensated Hypercapnic Respiratory Failure: A Randomized, Controlled Trial.

Int J Chron Obstruct Pulmon Dis 2020 24;15:3051-3061. Epub 2020 Nov 24.

Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of China.

Introduction: Currently, there is a lack of evidence on the utilization of high-flow nasal cannula (HFNC) in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) accompanied by hypercapnic respiratory failure. We aimed to explore the efficacy and safety of HFNC compared with conventional oxygen therapy (COT) in such patients.

Methods: This was a prospective, randomized, controlled trial. Patients with AECOPD with a baseline arterial blood gas pH ≥7.35, PaO <60 mmHg, and PaCO >45 mmHg were enrolled. The primary endpoint was treatment failure, which needs mechanical ventilation.

Results: A total of 320 patients were randomized to either the HFNC group (n = 160) or the COT group (n = 160). Sixteen (10.0%) patients in the HFNC group had treatment failure during hospitalization, which was significantly lower than the COT group figure of 31 (19.4%) patients ( = 0.026). Twenty-four hours after recruitment, the PaCO of the HFNC group was lower than that of the COT group (54.1 ± 9.79 mmHg vs 56.9 ± 10.1 mmHg, = 0.030). PaCO higher than 59 mmHg after HFNC for 24 h was identified as an independent risk factor for treatment failure [OR 1.078, 95% CI 1.006-1.154, = 0.032].

Conclusion: In AECOPD patients with acute compensated hypercapnic respiratory failure, HFNC improved the prognosis compared with COT. Therefore, HFNC might be considered for first-line oxygen therapy in select patients.

Trial Registration Number: ClinicalTrials.Gov: NCT02439333.
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http://dx.doi.org/10.2147/COPD.S283020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699989PMC
June 2021

Exercise pathophysiology differs between connective tissue diseases-associated pulmonary arterial hypertension and idiopathic pulmonary arterial hypertension.

Clin Exp Rheumatol 2020 Nov 10. Epub 2020 Nov 10.

Center for Pulmonary Vascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objectives: Previous studies demonstrated that connective tissue diseases-associated pulmonary arterial hypertension (CTD-PAH) had a worse prognosis than idiopathic pulmonary arterial hypertension (IPAH), although the former one had better haemodynamic profiles and right heart function. To find potential explanations for this contradictory phenomenon, we compared the exercise pathophysiology of CTD-PAH with that of IPAH using cardiopulmonary exercise testing (CPET).

Methods: Ninety-three CTD-PAH patients were retrospectively enrolled and matched 1:1 with 93 IPAH patients according to age, gender, body mass index, and body surface area. Multiple linear regression analysis was performed to adjust confounding factors.

Results: CTD-PAH had higher rest heart rate ([email protected]) and lower rest oxygen uptake/HR (VO2/[email protected]) than IPAH. During exercise, the peak power ([email protected]), [email protected], peak metabolic equivalents ([email protected]), peak minute ventilation ([email protected]), peak tidal volume ([email protected]), [email protected], peak systolic blood pressure ([email protected]) and peak diastolic blood pressure ([email protected]) of CTD-PAH were lower than those of IPAH. After adjustment, CTD-PAH still had lower values of [email protected], [email protected], [email protected], [email protected], VO2/[email protected], [email protected] and had higher [email protected] than IPAH.

Conclusions: CTD-PAH had more impaired ventilation, cardiac function and muscular strength (reflected by CPET-derived parameters) than IPAH, in despite of better haemodynamic profiles and comparable heart structure (assessed by echocardiography) and functional status (indicated by World Health Organisation functional class, N-terminal pro-brain natriuretic peptide and six-minute walk distance).
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November 2020

Quantification of artificial sweeteners in alcoholic drinks using direct analysis in real-time QTRAP mass spectrometry.

Food Chem 2021 Apr 8;342:128331. Epub 2020 Oct 8.

State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address:

Artificial sweeteners have been widely used as replacements for sugars in foods. Rapid determination of artificial sweeteners contained in various foods are highly desirable for the routine analysis. Here, we report a robust approach based on direct analysis in real time coupled with QTRAP mass spectrometry to screen and quantitate simultaneously seven artificial sweeteners, including aspartame, saccharin, acesulfame-K, neotame, sucralose, cyclamate and alitame in alcoholic drinks. The detection method merely involved a simple sample pretreatment process, with a good linearity, low limit of quantification, satisfied recovery and relative standard deviation for each target compound. More importantly, the approach is highly sensitive and accurate in monitoring the seven artificial sweeteners in whisky, Chinese liquors, beer and wines obtained from the supermarket. The results demonstrated that the approach described here could be suitable for large-scale application in routine quality control analysis of artificial sweeteners.
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http://dx.doi.org/10.1016/j.foodchem.2020.128331DOI Listing
April 2021

Nocturnal hypoxia in patients with idiopathic pulmonary arterial hypertension.

Pulm Circ 2020 Jul-Sep;10(3):2045894019885364. Epub 2020 Aug 21.

Center for Pulmonary Vascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Sleep-disordered breathing causes a variety of cardiovascular complications and increases the risk of a poor prognosis in patients. There is still some controversy regarding the clinical diagnosis and treatment of sleep-disordered breathing in patients with pulmonary hypertension. The aim of this study was to determine the incidence of desaturation in idiopathic pulmonary arterial hypertension (IPAH) patients, evaluate the effect of desaturation on the clinical status of patients with IPAH, and identify possible influencing factors.

Methods: Patients with IPAH diagnosed by right heart catheterization who underwent overnight cardiorespiratory monitoring from January 2018 to July 2019 were enrolled. Nocturnal hypoxic time was defined as the time that oxygen saturation remained below 90%. Desaturation was defined as a nocturnal oxygen saturation level less than 90% for more than 10% of the total recording time. Baseline clinical characteristics and parameters were collected to compare IPAH patients with and without desaturation. In addition, logistic regression was performed to identify possible factors associated with desaturation in IPAH patients.

Results: Fifty patients with IPAH were included. Among them, 17 patients presented desaturation. Patients with desaturation were older, had a shorter six-min walking distance (6MWD), had a higher mean right atrial pressure, and had a lower daytime arterial oxygen partial pressure than patients without desaturation, and there were significant differences in the VE/VCO and VE/VCO slope ( < 0.05). The multivariate logistic regression analysis indicated that the 6 MWD (OR = 0.971, 95% CI: 0.948-0.994,  = 0.013) and; VE/VCO slope (OR = 1.095, 95% CI: 1.010-1.307,  = 0.032) were independently associated with desaturation after adjusting for age, sex, and body mass index.

Conclusion: Nocturnal hypoxia is common in IPAH patients. Desaturation may aggravate the clinical situation of patients with IPAH. In IPAH patients, a poor exercise capacity (6 MWD) and the VE/VCO slope can predict desaturation after adjusting for age, sex, and body mass index.
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http://dx.doi.org/10.1177/2045894019885364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443990PMC
August 2020

Peripheral anterior chamber depth and screening techniques for primary angle closure disease in community elderly Chinese.

BMC Ophthalmol 2020 Aug 28;20(1):353. Epub 2020 Aug 28.

Department of Ophthalmology, Shanghai Eye Disease Prevention & Treatment Center/ Shanghai Eye Hospital, No. 380 Kangding Road, Shanghai, 200040, China.

Background: To investigate the distribution of peripheral anterior chamber depth (ACD) and the relationship between peripheral ACD and gonioscopy compared to other ocular parameters for primary angle closure disease (PACD) screening. We performed a population-based survey in Pudong New District of Shanghai, China, in 2011.

Methods: Cross-sectional study. Adults 50 and older were enrolled from a population-based study using cluster random sampling in Pudong New District, Shanghai. Remote ocular screening was performed with digital anterior eye structure photography. Van Herrick measurements were used to evaluate the peripheral ACD, the depth of the peripheral anterior chamber, and corneal thickness (CT), and the ACD to CT ratio was calculated. Subjects with peripheral ACD less than 0.5 CT were made follow-up appointments for clinical examination with gonioscopy. Receiver operating characteristic curves (ROC) were generated to show the performance of different tests in screening for primary angle closure disease (PACD).

Results: Two thousand five hundred twenty-eight adults participated in the study with 91 patients diagnosed with PACD. Two thousand four hundred sixty-three subjects had valid data in the right eye available for analysis. The mean peripheral ACD values for men and women were significantly different: 1.04 ± 0.46 (range 0.11-2.93) CT and 0.87 ± 0.41 (range 0.12-2.96) CT respectively (t = - 4.18; P<0.0001). Multivariate logistic regression analysis reveals that peripheral ACD declined by 0.31 CT (P < 0.0001) per diopter of SE and was 0.19 CT (P < 0.0001) shallower in women than in men (r2 = 0.1304, P < 0.0001). Peripheral ACD performed best in screening for PACD.

Conclusions: Peripheral ACD measurement is recommended for PACD screening in community elderly Chinese.
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http://dx.doi.org/10.1186/s12886-020-01618-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456037PMC
August 2020

[Clinical research on the surgical treatment of complex maxillary sinus diseases by pyriform aperture extend prelacimal recess approach].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2020 Apr;34(4):364-368

Department of Otolaryngology Head and Neck Surgery,the Second Affiliated Hospital of the University of South China,Hengyang,421001,China.

The aim of this study is to explore the operative method, indication and clinical effect of enlarging anterior lacrimal Fossa via Piriform foramen for complex lesions of maxillary Sinus. By imaging and pathology, twenty-one patients with tumors and bone cysts were confirmed that the lesion base was located in the anterior wall of maxillary sinus, alveolar recess and zygomatic recessor even more broadly. All patients were treated with pyriform aperture extend prelacimal recess approach surgery, and followed up for average 25 months. All the 21 operations went smoothly, including 11 cases diagnosed with papilloma, 4 cases diagnosed with maxillary sinus carcinoma, 1 case diagnosed with moderate dysplasia, 1 case diagnosed with fibroangioma, 1 case diagnosed with schwannomas, 1 case diagnosed with maxillary sinus osteoma, 1 case diagnosed with maxillary sinus bone cyst and 1 case diagnosed with dentigerous cyst. Pyriform aperture extend prelacimal recess approach can thoroughly deal with lesions located in each part of the maxillary sinus. Intraoperative blood loss(191.6±44.7) mL. After operations, 6 patients had mild facial swelling, and 8 cases experienced facial numbness; There were no facial scar, collapse and deformation, anterior nares narrowing, tears overflow and other complications. None of the patients relapsed. The enlarged anterior lacrimal fossa via piriform aperture can provide enough space for complete resection of the complex lesions of maxillary Sinus, which are located in anterior wall, Alveolar recess, zygomatic recess and various parts of the base, the postoperative reaction is light, the complication is few.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2020.04.019DOI Listing
April 2020
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