Publications by authors named "Qin Li"

2,329 Publications

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Corrigendum: Neuroprotective Effects of the Anti-Cancer Drug Lapatinib Against Epileptic Seizures Suppressing Glutathione Peroxidase 4-Dependent Ferroptosis.

Front Pharmacol 2021 3;12:810295. Epub 2022 Jan 3.

Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, China.

[This corrects the article DOI: 10.3389/fphar.2020.601572.].
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http://dx.doi.org/10.3389/fphar.2021.810295DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8762335PMC
January 2022

Integrative genomic and transcriptomic analysis reveals immune subtypes and prognostic markers in ovarian clear cell carcinoma.

Br J Cancer 2022 Jan 18. Epub 2022 Jan 18.

Department of Gynecologic Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.

Background: We performed an integrative genomic and transcriptomic profiling to identify molecular subtypes and prognostic markers with special focus on immune-related pathways.

Methods: Totally, 50 Chinese patients were subjected to targeted next-generation sequencing and transcriptomic sequencing.

Results: Two distinct subgroups were identified as immune (22.0%) and non-immune (78.0%) based on the immune-pathway related hierarchical clustering. Surprisingly, patients with immune subtype had a significantly worse survival. The prognostic capacity was validated in external cohorts. The immune group had higher expression of genes involved in pro-inflammation and checkpoints. PD-1 signalling pathway was enriched in the immune subtype. Besides, the immune cluster presented enriched expression of genes involved in epithelial-mesenchymal transition, angiogenesis and PI3K-AKT-mTOR signalling, while the non-immune subtype had higher expression of metabolic pathways. The immune subtype had a higher mutation rate of PIK3CA though significance was not achieved. Lastly, we established a prognostic immune signature for overall survival. Interestingly, the immune signature could also be applied to renal clear cell carcinoma, but not to other histologic subtype of ovarian cancer.

Conclusions: An immune subtype of OCCC was identified with poor survival and enrichment of PD-1 and PI3K-AKT-mTOR signalling. We constructed and validated a robust prognostic immune signature of OCCC patients.
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http://dx.doi.org/10.1038/s41416-022-01705-wDOI Listing
January 2022

Heteroresistance to Amikacin in Carbapenem-Resistant Strains.

Front Microbiol 2021 2;12:682239. Epub 2021 Dec 2.

Department of Pathogen Biology, School of Basic Medicine, Public Center of Experimental Technology of Pathogen Biology Technology Platform, Southwest Medical University, Luzhou, China.

Heteroresistance can lead to treatment failure and is difficult to detect by the methods currently employed by clinical laboratories. The aim of this study was to investigate the prevalence of the amikacin-heteroresistant strains and explore potential amikacin heteroresistance mechanism through whole-genome sequencing (WGS) and quantitative reverse-transcription PCR (qRT-PCR). In this study, 13 isolates (8.39%) were considered as amikacin-heteroresistant strains among a total of 155 strains. The majority of the heterogeneous phenotypes (11/13, 84.61%) was unstable and the minimal inhibitory concentrations (MICs) fully or partially reverted back to the level of susceptibility of the parental isolate. The frequency of heteroresistant subpopulation ranged from 2.94×10 to 5.59×10. Whole-genome sequencing and single-nucleotide variants (SNVs) analysis showed that there were different nucleotide and resultant amino acid alterations among an amikacin-heteroresistant strain S38 and the resistant subpopulation S38L in several genes. Quantitative reverse-transcription PCR analysis revealed that the increased expression of aminoglycoside resistance genes detected in amikacin-heteroresistant strains might be associated with amikacin heteroresistance. The findings raise concerns for the emergence of amikacin-heteroresistant strains and the use of amikacin as therapy for the treatment of multidrug-resistant strains.
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http://dx.doi.org/10.3389/fmicb.2021.682239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8753984PMC
December 2021

Cell components, interaction types and functional groups involved in the in vitro binding of bisphenol A by Lactiplantibacillus plantarum RS20D and DL7X.

J Appl Microbiol 2022 Jan 13. Epub 2022 Jan 13.

College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China.

Aims: The current study aimed to evaluate the capacity of two Lactiplantibacillus plantarum strains to remove Bisphenol A (BPA) and to determine the preliminary removal mechanisms underlying this process.

Methods And Results: The BPA removal capacity of L. plantarum RS20D and DL7X was assessed by HPLC analysis. The effect of various treatments (physical, chemical, and enzymatic) on two strains were studied to understand which interaction types worked. The different cellular components of them were also subjected to binding assays. Additionally, Fourier-transform infrared spectroscopy (FTIR) was performed to identify the functional groups related to the BPA binding process. Results show that various treatments enhanced the binding capacity of two strains, the effect of sodium dodecyl sulfate was the most outstanding (P=0.000, P=0.000). Hydrogen bonding and hydrophobic interactions likely occurred. Peptidoglycans showed the highest binding capability, protoplasts and teichoic acids might also exert a binding effect. -OH, C=O, -CH, -NH, C-N, C-O, and P=O participated in BPA binding by the two L. plantarum lines.

Conclusions: Peptidoglycans, protoplasts, and teichoic acid played a vital role in the binding of BPA.

Significance And Impact Of The Study: Our results provided a theoretical foundation for developing effective dietary strategies with foodborne L. plantarum to remove food contaminants.
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http://dx.doi.org/10.1111/jam.15450DOI Listing
January 2022

CircRNA casein kinase 1 gamma 1 (circ-CSNK1G1) plays carcinogenic effects in thyroid cancer by acting as miR-149-5p sponge and relieving the suppression of miR-149-5p on mitogen-activated protein kinase 1 (MAPK1).

J Clin Lab Anal 2022 Jan 13:e24188. Epub 2022 Jan 13.

Department of Endocrine-metabolic, Yichun People's Hospital, Yichun, China.

Background: The initiation and development of thyroid cancer may be associated with the deregulation of circular RNAs (circRNAs). The purpose of this work was to explore the role of circRNA casein kinase 1 gamma 1 (circ-CSNK1G1) in thyroid cancer.

Methods: The expression of circ-CSNK1G1, miR-149-5p, and mitogen-activated protein kinase 1 (MAPK1) was concluded using quantitative real-time PCR (qPCR), and the expression of MAPK1 protein was detected by Western blot assay. Cell viability was monitored by CCK-8 assay. Cell proliferation was determined by colony formation assay and EdU assay. Cell apoptosis and cycle were checked by flow cytometry assay. Cell invasion was determined by transwell assay. The predicted binding relationship between miR-149-5p and circ-CSNK1G1 or MAPK1 was verified by dual-luciferase reporter assay. The role of circ-CSNK1G1 in vivo was determined by establishing animal models.

Results: The present work discovered the upregulation of circ-CSNK1G1 in tumor tissues of thyroid cancer. In function, circ-CSNK1G1 knockdown inhibited proliferation, survival, and invasion in cancer cells, and tumor growth in mouse models. MiR-149-5p was a target of circ-CSNK1G1, and the anti-tumor effects of circ-CSNK1G1 knockdown were abolished by miR-149-5p downregulation. In addition, miR-149-5p directly targeted MAPK1, and miR-149-5p restoration-inhibited cell proliferation and invasion were recovered by MAPK1 overexpression.

Conclusion: Circ-CSNK1G1 acted as miR-149-5p to relieve the inhibition of miR-149-5p on MAPK1, thus promoting the malignant development of thyroid cancer.
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http://dx.doi.org/10.1002/jcla.24188DOI Listing
January 2022

Emerione A, a novel fungal metabolite as an inhibitor of New Delhi metallo-β-lactamase-1, restores carbapenem susceptibility in carbapenem-resistant isolates.

J Glob Antimicrob Resist 2022 Jan 8. Epub 2022 Jan 8.

Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China; Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China. Electronic address:

Objectives: Bacterial strains that produce New Delhi metal-β-lactamase 1 (NDM-1) are worldwide threats. It is still a challenging task to find a potent NDM-1 inhibitor for clinical practice.

Methods: Molecular docking and virtual screening of an in-house fungal natural product database for NDM-1 inhibitors were performed. Based on the screening results, the affinity and inhibition analysis of potential NDM-1 inhibitors was determined using purified NDM-1. The efficacy of compounds in combination with four β-lactam antibiotics (meropenem, imipenem, ceftriaxone and ampicillin) was evaluated. The morphological transforms of K. pneumoniae ATCC BAA2146 after treatment with the compounds were visualized by transmission electron microscopy.

Results: In silico screening led to the identification of four fungal products as potential NDM-1 inhibitors. Emerione A (1), a methylated polyketide with bicyclo[4.2.0]octene and 3,6-dioxabicyclo[3.1.0]hexane, has significant activity in cells (K = 11.8 ± 0.6 μM; IC = 12.1 ± 0.9 μM) and potentiates the activity of meropenem against two kinds of NDM-1-producing Enterobacteriaceae. To the best of our knowledge, emerione A (1) is the second fungal metabolite reported to exhibit NMD-1 inhibitory activity. According to the structural novelty of our database, we also found a structural new compound, asperfunolone A (2), with potential NMD-1 inhibitory activity.

Conclusion: Considering the low toxicity characteristic of emerione A (1), it may be processed as a potential lead compound for anti-NDM-1 drug development.
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http://dx.doi.org/10.1016/j.jgar.2021.12.019DOI Listing
January 2022

Illness perception and treatment experience in patients with gout: a descriptive qualitative study.

Clin Rheumatol 2022 Jan 11. Epub 2022 Jan 11.

School of Nursing, Qingdao University, Qingdao, Shandong, China.

Objective: To explore the illness perception and treatment experience of gout patients in China.

Methods: A descriptive qualitative approach was used. In-depth semi-structured individual interviews were conducted among 18 adults with gout between August 2020 and December 2020. The Common-Sense Model of Self-Regulation was used as the conceptual framework for developing interview guide and data analysis. Interviews were audio recorded, transcribed, independently coded, and analyzed for themes.

Results: The 18 gout patients aged 23-77 years old, with 16 being male. Four themes were generated to reflect the experience of patients with gout: limited knowledge of gout, various but inadequate sources of gout knowledge, multifaceted influences of gout, and seeking for diversified disease treatment methods.

Conclusion: There is a gap between patients' anticipation and practical service that is closely linked with subsequent poor gout management among the participants. The findings indicate a need of strengthening health education about gout by considering building nurse-led support groups and developing mobile health applications, as well as addressing the long-term influence of the Chinese alcoholic drinking culture to promote patients' effective disease management.

Key Points: • This qualitative study fills a knowledge gap on illness perception and treatment experience among Chinese gout patients, exploring how these patients perceived gout and manage gout. • The gout patients in this study reported disparity between personal anticipation and healthcare service that largely contributes to their poor gout management. • The Chinese alcoholic drinking culture embedded in personal, professional, and social activities posed extra difficulties and challenges on modifying lifestyle for gout patients that needs to be addressed for promoting gout management among this group.
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http://dx.doi.org/10.1007/s10067-021-06014-wDOI Listing
January 2022

Emergency Management of Medical Wastewater in Hospitals Specializing in Infectious Diseases: A Case Study of Huoshenshan Hospital, Wuhan, China.

Int J Environ Res Public Health 2021 12 30;19(1). Epub 2021 Dec 30.

School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430205, China.

Medical wastewater originating from hospitals specializing in infectious diseases pose a major risk to human and environmental health during pandemics. However, there have been few systematic studies on the management of this type of wastewater management. The function of the Huoshenshan Hospital as a designated emergency field hospital for the treatment of COVID-19 has provided lessons for the management measures of medical wastewater, mainly including: (1) Modern information technology, management schemes, and related standard systems provided the legislative foundation for emergency management of medical wastewater. (2) The three-tier prevention and control medical wastewater management system ensured the discharged wastewater met water quality standards, especially for the leak-proof sealed collection system of the first tier, and the biological and chemical treatment technology of the second tier. (3) The establishment of an effective three-tier medical wastewater quality monitoring accountability system. This system was particularly relevant for ensuring continuous data monitoring and dynamic analysis of characteristic indicators. (4) Information disclosure by government and public supervision promoted successful implementation of medical wastewater management and control measures. Public questionnaires (n = 212) further confirmed the effectiveness of information disclosure. The results of this study can act as methodological reference for the emergency management of wastewater in designated infectious disease hospitals under similar situations.
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http://dx.doi.org/10.3390/ijerph19010381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751112PMC
December 2021

Titanium Implants and Local Drug Delivery Systems Become Mutual Promoters in Orthopedic Clinics.

Nanomaterials (Basel) 2021 Dec 24;12(1). Epub 2021 Dec 24.

The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China.

Titanium implants have always been regarded as one of the gold standard treatments for orthopedic applications, but they still face challenges such as pain, bacterial infections, insufficient osseointegration, immune rejection, and difficulty in personalizing treatment in the clinic. These challenges may lead to the patients having to undergo a painful second operation, along with increased economic burden, but the use of drugs is actively solving these problems. The use of systemic drug delivery systems through oral, intravenous, and intramuscular injection of various drugs with different pharmacological properties has effectively reduced the levels of inflammation, lowered the risk of endophytic bacterial infection, and regulated the progress of bone tumor cells, processing and regulating the balance of bone metabolism around the titanium implants. However, due to the limitations of systemic drug delivery systems-such as pharmacokinetics, and the characteristics of bone tissue in the event of different forms of trauma or disease-sometimes the expected effect cannot be achieved. Meanwhile, titanium implants loaded with drugs for local administration have gradually attracted the attention of many researchers. This article reviews the latest developments in local drug delivery systems in recent years, detailing how various types of drugs cooperate with titanium implants to enhance antibacterial, antitumor, and osseointegration effects. Additionally, we summarize the improved technology of titanium implants for drug loading and the control of drug release, along with molecular mechanisms of bone regeneration and vascularization. Finally, we lay out some future prospects in this field.
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http://dx.doi.org/10.3390/nano12010047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746425PMC
December 2021

Nb/Sn Liquid-Solid Reactive Diffusion Couples and Their Application to Determination of Phase Equilibria and Interdiffusion Coefficients of Nb-Sn Binary System.

Materials (Basel) 2021 Dec 24;15(1). Epub 2021 Dec 24.

State Key Lab of Powder Metallurgy, Central South University, Changsha 410083, China.

NbSn plays an irreplaceable role in superconducting parts due to its stable performance under high field conditions. Accurate phase equilibria and interdiffusion coefficients are of great significance for designing novel NbSn superconductors. However, the related experimental information is still in a state of scarcity because of the difficulty in fabrication of Nb-Sn alloys caused by the large difference in melting points of Nb and Sn. In this paper, a simple but pragmatic approach was first proposed to prepare the Nb/Sn liquid-solid reactive diffusion couples (LSDCs) at 1100 °C and 1200 °C, of which the phase identification of the formed layer and the measurement of composition-distance profiles were conducted. The formed layer in Nb/Sn LSDCs was confirmed to be NbSn compound. While the measured composition profiles were employed to determine the phase equilibria according to the local equilibrium hypothesis and the interdiffusion coefficients with an aid of the latest version of HitDIC software. The determined phase equilibria of NbSn, (Nb) and liquid show good agreement with the assessed phase diagram. While the calculated interdiffusion coefficients and activation energy for diffusion in NbSn are consistent with both experimental and theoretical data in the literature. Moreover, the growth of the formed NbSn layer in Nb/Sn LSDCs was also found to be diffusion controlled. All the obtained phase equilibria and interdiffusion coefficients are of great value for further thermodynamic and kinetic modeling of the Nb-Sn system. Furthermore, it is anticipated that the presently proposed approach of fabricating liquid-solid reactive diffusion couple should serve as a general one for various alloy systems with large differences in melting points.
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http://dx.doi.org/10.3390/ma15010113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746297PMC
December 2021

Non-Coding RNA m6A Modification in Cancer: Mechanisms and Therapeutic Targets.

Front Cell Dev Biol 2021 22;9:778582. Epub 2021 Dec 22.

Department of Clinical Pharmacy, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Recently, N6-methyl-adenosine (m6A) ribonucleic acid (RNA) modification, a critical and common internal RNA modification in higher eukaryotes, has generated considerable research interests. Extensive studies have revealed that non-coding RNA m6A modifications (e.g. microRNAs, long non-coding RNAs, and circular RNAs) are associated with tumorigenesis, metastasis, and other tumour characteristics; in addition, they are crucial molecular regulators of cancer progression. In this review, we discuss the relationship between non-coding RNA m6A modification and cancer progression from the perspective of various cancers. In particular, we focus on important mechanisms in tumour progression such as proliferation, apoptosis, invasion and metastasis, tumour angiogenesis. In addition, we introduce clinical applications to illustrate more vividly that non-coding RNA m6A modification has broad research prospects. With this review, we aim to summarize the latest insights and ideas into non-coding RNA m6A modification in cancer progression and targeted therapy, facilitating further research.
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http://dx.doi.org/10.3389/fcell.2021.778582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728017PMC
December 2021

CYP1B1-AS1 Is a Novel Biomarker in Glioblastoma by Comprehensive Analysis.

Dis Markers 2021 29;2021:8565943. Epub 2021 Dec 29.

Department of Neurology, Chongqing General Hospital, University of Chinese Academy of Sciences, Liangjiang New Area, Chongqing, China.

Objective: Growing evidence shows that enhancer RNAs (eRNAs) are pivotal for tumor progression. In this research, our team aimed to identify the survival-related eRNAs and further explore their potential function in glioblastoma (GBM).

Methods: RNA-sequencing data in 31 tumor types were acquired from TCGA datasets. The survival-related eRNAs were identified by the use of Kaplan-Meier survival analyses and Spearman's correlation analyses. KEGG pathway enrichment analysis was completed to investigate the underlying signal paths of the critical eRNA. Pancancer assays were applied to explore the association between CYP1B1-AS1 and CYP1B1.

Results: We identified 74 survival-related eRNAs and focused on CYP1B1-AS1 which displayed the greatest cor value. CYP1B1 was identified as a regulatory target of CYP1B1-AS1. KEGG analyses suggested that CYP1B1-AS1 might play an essential role through CK-CKR mutual effect, complement and coagulation cascades, TNF signal path, and JAK-STAT signal path. The pancancer verification outcomes revealed that CYP1B1-AS1 was related to survival in 4 cancers, i.e., LIHC, KIRP, KICH, and KIRC. Association was discovered between CYP1B1-AS1 and the targeted gene, CYP1B1, in 29 cancer types.

Conclusion: The outcomes herein provided the first evidence that overexpression of CYP1B1-AS1 might be a potential molecular biomarker for predicting the prognosis of patients with GBM.
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http://dx.doi.org/10.1155/2021/8565943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733712PMC
December 2021

Platelet-Tumor Cell Hybrid Membrane-Camouflaged Nanoparticles for Enhancing Therapy Efficacy in Glioma.

Int J Nanomedicine 2021 29;16:8433-8446. Epub 2021 Dec 29.

Department of Transfusion Medicine, Allergy Center, Ministry of Education Key Laboratory of Laboratory Medicine, Zhejiang Provincial People's Hospital People's Hospital of Hangzhou Medical College, Hangzhou, 310014, People's Republic of China.

Purpose: Cell membrane-camouflaged nanoparticles (NPs) are drawing increasing attention because their surfaces acquire some characteristics of the cell membranes, making them a unique class of biomimetic materials for diverse applications. Modification of cell membrane or combination of different types of membranes can enhance their functionality.

Methods: We prepared platelet and tumor cell membrane camouflaged β-mangostin-loaded NPs, which were synthesized with platelet-C6 hybrid biomimetic coating, poly(lactic-co-glycolic acid), and β-mangostin (β-PCNPs). Then, we evaluated their targeting ability and anticancer activity against glioma in vitro and in vivo.

Results: Biomimetic coating enhanced active drug targeting and immune escape properties of nanocarrier in C6 and THP-1 cells, respectively, which improved their cytotoxicity. β-PCNPs were characterized to study the inherent properties of both source cells. Compared with bare β-NPs, β-PCNPs exhibited high tumor-targeting capability and induced apoptosis of C6 cells in vitro. Similarly, intravenous administration of drug through β-PCNPs resulted in enhanced tumor-targeting and exhibited excellent rate of inhibition of glioma tumor growth in mice. Moreover, the blood circulation time of drug in mice in the β-PCNP group was markedly prolonged and these mice exhibited better outcome than those in the β-NP group.

Conclusion: These results provide a new strategy of utilizing PCNPs as carriers for drug delivery, which improves the targeting efficiency and therapeutic efficacy of chemotherapeutic agents for glioma therapy.
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http://dx.doi.org/10.2147/IJN.S333279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727453PMC
January 2022

Urinary monohydroxylated polycyclic aromatic hydrocarbons in the general population from 26 provincial capital cities in China: Levels, influencing factors, and health risks.

Environ Int 2022 Jan 4;160:107074. Epub 2022 Jan 4.

Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, PR China; Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, Key Laboratory of City Cluster Environmental Safety and Green Development, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, PR China. Electronic address:

Polycyclic aromatic hydrocarbons (PAHs) derived from the incomplete combustion of organic materials are associated with adverse health effects. However, little is known about PAH exposure levels and their influencing factors on a large scale in developing countries. In this study, urinary monohydroxylated metabolites of PAHs (OH-PAHs), including the metabolites of naphthalene, fluorene, phenanthrene, pyrene, chrysene, and benzo[a]pyrene, were measured in 1154 samples in the general population nationwide from 26 provincial capitals in China. Concentrations of OH-PAHs ranged from 1.39 to 228 μg/L. OH-Nap, metabolite of naphthalene, was the predominant compound, accounting for 65.1% of totals. People in eastern, southwest and northeast China, such as Shanghai, Kunming, Nanning, and Changchun, suffered more PAH exposure than other regions which might associate with sampling time, living habits of the subjects, and the imbalance of economic development and energy consumption across regions. Urinary OH-PAH concentrations were associated with body mass index, gender, and age, and smoking was the main correlating factor. Inhalation and diet might be the main exposure route of human exposure to PAHs, especially for smokers by inhalation. Hazard indices showed that no subject was exposed to PAHs with potential non-carcinogenic risk. Furthermore, the carcinogenic risk was the most significant health effects, with almost all subjects having carcinogenic risk values higher than the acceptable level of 10. Naphthalene and phenanthrene were the main contributors. The results also suggested a possible relationship between PAH exposure and lung cancer in the Chinese population. This first nationwide study on human internal exposure to PAHs provides a large body of scientific information for governmental decision-making about associated human health and the prevention of human exposure to PAHs.
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http://dx.doi.org/10.1016/j.envint.2021.107074DOI Listing
January 2022

ASTE1 frameshift mutation triggers the immune response in Epstein-Barr virus-associated gastric cancer.

Signal Transduct Target Ther 2022 Jan 5;7(1). Epub 2022 Jan 5.

Department of Gastric Surgery, Fudan University Shanghai Cancer Center, and Shanghai Key Laboratory of Medical Epigenetics, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

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http://dx.doi.org/10.1038/s41392-021-00771-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8727626PMC
January 2022

Application of L. Extract at the Early Stage of Tumor Development Helps Cyclophosphamide Inhibit the Growth of Tumor Cells.

Biomed Environ Sci 2021 Dec;34(12):1010-1014

Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun 130021, Jilin, China.

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http://dx.doi.org/10.3967/bes2021.136DOI Listing
December 2021

Common Genomic Aberrations in Mouse and Human Breast Cancers with Concurrent P53 Deficiency and Activated PTEN-PI3K-AKT Pathway.

Int J Biol Sci 2022 1;18(1):229-241. Epub 2022 Jan 1.

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030.

Simultaneous P53 loss and activation of the PTEN-restricted PI3K-AKT pathway frequently occur in aggressive breast cancers. P53 loss causes genome instability, while PTEN loss and/or activating mutations of PIK3CA and AKT promote cancer cell proliferation that also increases incidences of genomic aberrations. However, the genomic alterations associated with P53 loss and activated PTEN-PI3K-AKT signaling in breast cancer have not been defined. Spatiotemporally controlled breast cancer models with inactivation of both P53 and Pten in adult mice have not been established for studying genomic alterations. Herein, we deleted both floxed and genes in the mammary gland epithelial cells in adult mice using a RCAS virus-mediated Cre-expressing system. These mice developed small tumors in 21 weeks, and poorly differentiated larger tumors in 26 weeks. In these tumors, we identified 360 genes mutated by nonsynonymous point mutations and small insertions and deletions (NSPMs/InDels), 435 genes altered by copy number amplifications (CNAs), and 450 genes inactivated by copy number deletions (CNDs). Importantly, 22.2%, 75.9% and 27.3% of these genes were also altered in human breast tumors with P53 and PTEN losses or P53 loss and activated PI3K-AKT signaling by NSPMs/InDels, CNAs and CNDs, respectively. Therefore, inactivation of P53 and Pten in adult mice causes rapid-growing breast tumors, and these tumors recapitulate a significant number of genetic aberrations in human breast tumors with inactivated P53 and activated PTEN-PI3K-AKT signaling. Further characterization of these commonly altered genes in breast cancer should help to identify novel cancer-driving genes and molecular targets for developing therapeutics.
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http://dx.doi.org/10.7150/ijbs.65763DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692138PMC
January 2022

Knockdown of XIST up-regulates 263294miR-340-5p to relieve myocardial ischaemia-reperfusion injury via inhibiting cyclin D1.

ESC Heart Fail 2021 Dec 30. Epub 2021 Dec 30.

Department of Cardiovascular Medicine Ward II, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 16 North Tongbai Road, Zhongyuan District, Zhengzhou, Henan, 450000, China.

Aim: Long non-coding RNAs (lncRNAs) are known to participate in various human diseases, while the role of X inactive-specific transcript (XIST) binding microRNA-340-5p (miR-340-5p) remains seldom studied. We aim to identify the role of the XIST/miR-340-5p/cyclin D1 (CCND1) axis in the myocardial ischaemia-reperfusion injury (MIRI).

Methods And Results: The mouse MIRI models were established. The expression of XIST, miR-340-5p, and CCND1 in mouse myocardial tissues in MIRI mice was assessed. The MIRI mice were respectively treated with altered XIST, miR-340-5p, or CCND1. The changes of myocardial enzyme activity were assessed, and the cardiac function was evaluated. Myocardial pathological changes, cardiomyocyte apoptosis and related apoptotic factors, oxidative stress and inflammatory factors were observed in myocardial tissues in mice with MIRI. The binding relationships between XIST and miR-340-5p, and between miR-340-5p and CCND1 were confirmed. XIST and CCND1 were up-regulated while miR-340-5p was down-regulated in MIRI mice. Silenced XIST could elevated miR-340-5p expression and reduced CCND1 expression, so as to promoted cardiac function and suppressed myocardial enzyme activity, ameliorated pathological changes, decelerated cardiomyocyte apoptosis by elevating Bcl-2 but reducing the levels of Bax and Caspase-3, attenuated inflammatory response by repressing IL-6 and TNF-α levels, and mitigated oxidative stress by reducing MDA contents and increasing CAT, GSH-Px, and SOD levels in MIRI mice. XIST sponged miR-340-5p and miR-340-5p targeted CCND1.

Conclusions: Knockdown of XIST up-regulates miR-340-5p to relieve MIRI via inhibiting CCND1.
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http://dx.doi.org/10.1002/ehf2.13766DOI Listing
December 2021

Knowledge, attitudes and practices related to influenza and influenza vaccine among healthcare workers in Chongqing, China-a cross-sectional study.

Hum Vaccin Immunother 2021 Dec 29:1-9. Epub 2021 Dec 29.

School of Public Health and Management, Collaborative Innovation Center of Social Risks Governance in Health, Chongqing Medical University, Chongqing, China.

Influenza is associated with a substantial disease burden, and influenza vaccination is recommended to all healthcare workers. We aimed to assess healthcare workers' knowledge, attitudes, and practices about influenza and its vaccine in Chongqing, China. A cross-sectional study was conducted at selected hospitals from September to November 2019, in which healthcare workers filled in a self-administered questionnaire. Both sentinel (42.92%) and non-sentinel hospitals (57.08%) were included. The majority were nurses (52.48%) and physicians (32.37%). Half (50.42%) of the respondents had a good command of knowledge, and the proportion of healthcare workers having a positive attitude accounted for 62.68%. The primary information sources were colleagues (58.81%), followed by television, newspapers and media (30.18%). The number of healthcare workers reported having got vaccinated last year was only 237 (16.78%), and the main reason was protecting themselves from influenza (93.25%). While the most common reasons given for not getting vaccinated were having no time (65.70%), believing it is unnecessary to get vaccinated (29.62%), worrying about the quality of influenza vaccine (27.49%) or the adverse reactions (25.70%). Factors associated with self-reported high vaccination were sentinel hospital (aOR: 1.427; 95% CI: 1.057-1.925), high-risk department (aOR: 1.919; 95% CI: 1.423-2.589), positive attitude (aOR: 2.429; 95% CI: 1.697-3.477) and taking the initiative to learn influenza information (aOR: 3.000; 95% CI: 1.983-4.538). We concluded that healthcare workers in Chongqing had some misconceptions although many of them showed a positive attitude toward the influenza vaccine. Various strategies, including educational training and on-site vaccination, are necessary to improve the knowledge and overall vaccination coverage.
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http://dx.doi.org/10.1080/21645515.2021.2007013DOI Listing
December 2021

Comment on: Cardiovascular events in adult polymyositis and dermatomyositis: a meta-analysis of observational studies.

Authors:
Li Qin Han Wang

Rheumatology (Oxford) 2021 Dec 28. Epub 2021 Dec 28.

Department of Cardiology, The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu, China.

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http://dx.doi.org/10.1093/rheumatology/keab951DOI Listing
December 2021

The Association between Outdoor Ambient Temperature and the Risk of Low Birth Weight: A Population-Based Cohort Study in Rural Henan, China.

Biomed Environ Sci 2021 Nov;34(11):905-909

Department of Environmental Health & Environment and Health Innovation Team, School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China.

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http://dx.doi.org/10.3967/bes2021.124DOI Listing
November 2021

Performance of a deep learning-based CT image denoising method: Generalizability over dose, reconstruction kernel, and slice thickness.

Med Phys 2021 Dec 25. Epub 2021 Dec 25.

Center for Devices and Radiological Health, US Food and Drug Administration (FDA), Silver Spring, Maryland, USA.

Purpose: Deep learning (DL) is rapidly finding applications in low-dose CT image denoising. While having the potential to improve the image quality (IQ) over the filtered back projection method (FBP) and produce images quickly, performance generalizability of the data-driven DL methods is not fully understood yet. The main purpose of this work is to investigate the performance generalizability of a low-dose CT image denoising neural network in data acquired under different scan conditions, particularly relating to these three parameters: reconstruction kernel, slice thickness, and dose (noise) level. A secondary goal is to identify any underlying data property associated with the CT scan settings that might help predict the generalizability of the denoising network.

Methods: We select the residual encoder-decoder convolutional neural network (REDCNN) as an example of a low-dose CT image denoising technique in this work. To study how the network generalizes on the three imaging parameters, we grouped the CT volumes in the Low-Dose Grand Challenge (LDGC) data into three pairs of training datasets according to their imaging parameters, changing only one parameter in each pair. We trained REDCNN with them to obtain six denoising models. We test each denoising model on datasets of matching and mismatching parameters with respect to its training sets regarding dose, reconstruction kernel, and slice thickness, respectively, to evaluate the denoising performance changes. Denoising performances are evaluated on patient scans, simulated phantom scans, and physical phantom scans using IQ metrics including mean-squared error (MSE), contrast-dependent modulation transfer function (MTF), pixel-level noise power spectrum (pNPS), and low-contrast lesion detectability (LCD).

Results: REDCNN had larger MSE when the testing data were different from the training data in reconstruction kernel, but no significant MSE difference when varying slice thickness in the testing data. REDCNN trained with quarter-dose data had slightly worse MSE in denoising higher-dose images than that trained with mixed-dose data (17%-80%). The MTF tests showed that REDCNN trained with the two reconstruction kernels and slice thicknesses yielded images of similar image resolution. However, REDCNN trained with mixed-dose data preserved the low-contrast resolution better compared to REDCNN trained with quarter-dose data. In the pNPS test, it was found that REDCNN trained with smooth-kernel data could not remove high-frequency noise in the test data of sharp kernel, possibly because the lack of high-frequency noise in the smooth-kernel data limited the ability of the trained model in removing high-frequency noise. Finally, in the LCD test, REDCNN improved the lesion detectability over the original FBP images regardless of whether the training and testing data had matching reconstruction kernels.

Conclusions: REDCNN is observed to be poorly generalizable between reconstruction kernels, more robust in denoising data of arbitrary dose levels when trained with mixed-dose data, and not highly sensitive to slice thickness. It is known that reconstruction kernel affects the in-plane pNPS shape of a CT image, whereas slice thickness and dose level do not, so it is possible that the generalizability performance of this CT image denoising network highly correlates to the pNPS similarity between the testing and training data.
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http://dx.doi.org/10.1002/mp.15430DOI Listing
December 2021

Added to pre-existing inflammation, mRNA-lipid nanoparticles induce inflammation exacerbation (IE).

J Control Release 2021 Dec 22. Epub 2021 Dec 22.

Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. Electronic address:

Current nucleoside-modified RNA lipid nanoparticle (modmRNA-LNP) technology has successfully paved the way for the highest clinical efficacy data from next-generation vaccinations against SARS-CoV-2 during the COVID-19 pandemic. However, such modmRNA-LNP technology has not been characterized in common pre-existing inflammatory or immune-challenged conditions, raising the risk of adverse clinical effects when administering modmRNA-LNPs to deliver therapeutic proteins or vaccinate against infectious diseases. Herein, we induce an acute-inflammation model in mice with lipopolysaccharide (LPS) intratracheally (IT), 1 mg kg, or intravenously (IV), 2 mg kg, and then IV administer modmRNA-LNP, 0.32 mg kg, after 4 h, and screen for inflammatory markers, such as pro-inflammatory cytokines. ModmRNA-LNP at this dose caused no significant elevation of cytokine levels in naive mice. In contrast, shortly after LPS immune stimulation, modmRNA-LNP enhanced inflammatory cytokine responses, Interleukin-6 (IL-6) in serum and Macrophage Inflammatory Protein 2 (MIP-2) in liver by 95-fold and 52-fold, respectively. Our report identifies this phenomenon as inflammation exacerbation (IE), which was proven to be specific to the LNP, acting independent of mRNA cargo, and was demonstrated to be time- and dose-dependent. Macrophage depletion and TLR3 -/- and TLR4-/- knockout mouse studies revealed macrophages were the immune cells involved or responsible for IE. Finally, we show that pretreatment with anti-inflammatory drugs, such as corticosteroids, can partially alleviate IE response in mice. Our findings characterize the importance of LNP-mediated IE phenomena in gram negative bacterial inflammation, the generalizability of modmRNA-LNP in other forms of chronic or acute inflammatory and immune contexts needs to be addressed.
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http://dx.doi.org/10.1016/j.jconrel.2021.12.027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8695324PMC
December 2021

Body composition in relation to postoperative anastomotic leakage and overall survival in patients with esophageal cancer.

Nutrition 2021 Nov 2;94:111534. Epub 2021 Nov 2.

Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:

Background: Body composition was reported to be related to the prognosis of patients with cancer. This study aimed to investigate the influence of preoperative body composition on anastomotic leakage and overall survival in patients with esophageal cancer.

Methods: In this retrospective study, 93 patients with esophageal cancers were evaluated. Skeletal muscle area, intermuscular adipose tissue, visceral adipose tissue (VAT), and subcutaneous adipose tissue were measured on computed tomography images at the level of the third lumbar vertebra. Subsequently, each body composition index was also calculated by dividing the body composition by the square of the height. The cut-off values of body compositions were defined using X-tile software (version 3.6.1; Yale University, New Haven, CTA). Univariate and multivariate analyses were performed to evaluate the risk factors of anastomotic leakage. Kaplan-Meier method and Cox regression analysis were used to evaluate the risk factors of overall survival.

Results: VAT and visceral fat index (VFI) were higher in patients with anastomotic fistula than in those without anastomotic fistula, but none of them were independent risk factors. Patients with higher body mass index (BMI), higher VFI, and higher subcutaneous fat index (SFI) had better overall survival. By multivariate analysis, SFI >27.6 cm/m was still significantly associated with overall survival.

Conclusion: Patients with higher VAT and VFI were prone to have an anastomotic leakage. Lower BMI, VFI, and SFI were associated with a reduction in overall survival.
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http://dx.doi.org/10.1016/j.nut.2021.111534DOI Listing
November 2021

A novel targeted co-delivery nanosystem for enhanced ovarian cancer treatment via multidrug resistance reversion and mTOR-mediated signaling pathway.

J Nanobiotechnology 2021 Dec 23;19(1):444. Epub 2021 Dec 23.

Henan Provincial People's Hospital, Zhengzhou, 450003, China.

Background: Multidrug resistance (MDR) is the main challenge of successful chemotherapy for ovarian cancer patients, with 50% to 75% of ovarian cancer patients eventually relapsed due to it. One of the effective strategies for treating MDR and improving therapeutic efficiency of ovarian cancer is to use nanotechnology-based targeted drug delivery systems. In this study, a novel nano targeted co-delivery system modified by hyaluronic acid (HA) was developed by using gold nanorods coated with functionalized mesoporous silica nanoparticles (HA-PTX/[email protected]) for combined delivery of hydrophobic chemotherapy drug Paclitaxel (PTX) and lethal-7a (let-7a), a microRNA (miR), to overcome MDR in ovarian cancer. Furthermore, we also analyzed the molecular mechanism of this nanotherapeutic system in the treatment of ovarian cancer.

Results: HA-modified nanocomplexes can specifically bind to the CD44 receptor, which is highly expressed in SKOV3/SKOV3 cells, achieving effective cell uptake and 150% enhancement of tumor site permeability. The nanosystem realized the stable combination and protective transportation of PTX and miRs. Analysis of drug-resistant SKOV3 cells and an SKOV3 xenograft model in BALB/c-nude mice showed significant downregulation of P-glycoprotein in heterogeneous tumor sites, PTX release, and subsequent induction of apoptosis. More importantly, this nanosystem could synergistically inhibit the growth of ovarian tumors. Further studies suggest that mTOR-mediated signaling pathways play an important role in reversing drug resistance and inducing apoptosis.

Conclusions: To sum up, these data provide a model for overcoming PTX resistance in ovarian cancer.
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http://dx.doi.org/10.1186/s12951-021-01139-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697442PMC
December 2021

The Two-Steps Reaction Fluorescent Probe for the Selective Detection of Cysteine and Its Applications.

Chem Biodivers 2021 Dec 21:e202100862. Epub 2021 Dec 21.

School of Pharmacy, Xinxiang, Medical University, Jinsui Road 601 Xinxiang, Henan, 453003, China.

We reported the specific fluorescent probe (MC-BOD-XDS) with two-steps reaction based on monosulfanyl-coumarin-BODIPY for selective detection of cysteine, high activity sulfanyl-coumarin as the multiple reaction group instead of a group internal standard fluorophore. The reaction mechanism of MC-BOD-XDS for detecting cysteine was different from the reported probes about the nucleophilic aromatic substitution reaction (SNAr) of chlorinated BODIPY. The fluorescent color of MC-BOD-XDS changed from yellow to red, and then to orange. The linear calibration diagram showed that it can potentially be used for quantitatively detection of Cys. Its potential applications were demonstrated by employing it for detection of Cys in artificial urine and in fluorescent imaging in HeLa cells.
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http://dx.doi.org/10.1002/cbdv.202100862DOI Listing
December 2021

Coordinatively Unsaturated Selenides over CuFeSe toward Highly Efficient Mercury Immobilization.

Environ Sci Technol 2022 Jan 21;56(1):575-584. Epub 2021 Dec 21.

School of Energy Science and Engineering, Central South University, Changsha 410083, China.

Metal selenides have been demonstrated as promising Hg remediators, while their inadequate adsorption rate primarily impedes their application feasibility. Based on the critical role of coordinatively unsaturated selenide ligands in immobilizing Hg, this work proposed a novel strategy to enhance the Hg adsorption rate of metal selenides by magnitudes by purposefully adjusting the selenide saturation. Copper iron diselenide (CuFeSe), in which the surface reconstruction tended to occur at ambient temperature, was adopted as the concentrator of unsaturated selenides. The adsorption rate of CuFeSe reached as high as 900.71 μg·g·min, far exceeding those of the previously reported metal selenides by at least 1 magnitude. The excellent resistance of CuFeSe to flue gas interference and temperature fluctuation warrants its applicability in real-world conditions. The theoretical investigations and mechanistic interpretations based on density functional theory (DFT) calculation further confirmed the indispensable role of unsaturated selenides in Hg adsorption. This work aims not only to develop a Hg remediator with extensive applicability in coal combustion flue gas but also to take a step toward the rational design of selenide-based sorbents for diverse environmental remediation by the facile surface functionalization of coordinatively adjustable ligands.
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http://dx.doi.org/10.1021/acs.est.1c05337DOI Listing
January 2022

Early versus delayed hip reduction in the surgical treatment of femoral head fracture combined with posterior hip dislocation: a comparative study.

BMC Musculoskelet Disord 2021 Dec 20;22(1):1057. Epub 2021 Dec 20.

Department of Orthopedics, West China Hospital, Sichuan University, 37# Guoxue Alley, Chengdu, 610041, Sichuan, People's Republic of China.

Background: Few studies focus on the treatment of femoral head fracture combined with posterior hip dislocation, and the safe interval time between injury and hip reduction remains controversial. The purpose of this study was to evaluate and compare the outcome of early and delayed hip reduction in the surgical treatment of femoral head fracture combined with posterior hip dislocation.

Methods: A total of 71 patients were evaluated in this retrospective study. Based on the time to hip reduction, they were divided into early group (within 6 h after injury) and delayed group (between 6 and 12 h after injury). The two groups were compared in reference to hospital day, fracture healing time, the occurrence of complications and final functional outcome. The Thompson-Epstein criteria, modified Merle D'Aubigné and Postel scores, visual analog scale (VAS) and Medical Outcomes Short Form 12-item questionnaire score (SF-12) were used for final functional evaluation.

Results: The mean hospital stay and fracture healing time in the early group were significantly lower than those in the delayed group. The incidence of infection, post-traumatic osteoarthritis, and avascular necrosis of the femoral head (ANFH) in the delayed group were higher than that in the early group. The early group had better functional outcomes in terms of Thompson-Epstein criteria, modified Merle D'Aubigné and Postel scores and physical component scale (PCS) than the delayed group.

Conclusions: For the treatment of femoral head fracture combined with posterior hip dislocation, the early and prompt hip reduction can effectively facilitate the fracture healing and patient rehabilitation, and obtain a better functional outcome.
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http://dx.doi.org/10.1186/s12891-021-04968-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8690980PMC
December 2021

Aqueous angiopoietin-like levels correlate with optical coherence tomography angiography metrics in diabetic macular edema.

Int J Ophthalmol 2021 18;14(12):1888-1894. Epub 2021 Dec 18.

Department of Ophthalmology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.

Aim: To quantitatively detect aqueous levels of angiopoietin-like (ANGPTL)3, ANGPTL4, and ANGPTL6 and investigate their correlation with optical coherence tomography angiography (OCTA) findings in patients with diabetic macular edema (DME).

Methods: This cross-sectional study included 23 patients (27 eyes) with type 2 diabetes and 16 control subjects (20 eyes). All patients underwent OCTA imaging and ultra-wide field fundus photography. Diabetic patients were categorized into two groups according to the presence or absence of diabetic retinopathy (DME group, 14 patients, 16 eyes); and non-diabetic retinopathy (NDR) group, 9 patients, 11 eyes, respectively. Aqueous levels of ANGPTL3, ANGPTL4, and ANGPTL6 were assessed using suspension array technology, and foveal-centered 3×3 mm OCTA scans were automatically graded to determine the central, inner, and full vessel density (CVD, IVD, FVD); central, inner, and full perfusion density (CPD, IPD, FPD), foveal avascular zone (FAZ) area, FAZ perimeter, and FAZ circularity index (FAZ-CI) on superficial capillary plexuses. Additionally, central subfield thickness (CST), cube volume (CV), and cube average thickness (CAT) were measured in a model of macular cube 512×128.

Results: Aqueous ANGPTL3 levels were not significantly different among the three groups (>0.05). ANGPTL4 levels were significantly higher in the DME group than the control and NDR groups (<0.0001 and <0.001), while ANGPTL6 levels were significantly higher in the DME group than the control group (<0.05). In the whole cohort, the aqueous ANGPTL3 levels correlated negatively with the IVD, FVD, IPD, and FPD, and positively with the CV and CAT. The aqueous ANGPTL4 levels correlated negatively with the CVD, IVD, FVD, CPD, IPD, and FPD, and positively with the FAZ perimeter, CST, CV, and CAT. The aqueous ANGPTL6 levels correlated negatively with the IVD, FVD, IPD, FPD, FAZ-CI and positively with CST, CV, CAT.

Conclusion: ANGPTL4 and ANGPTL6 may be associated with vascular leakage in DME and may represent good targets for DME therapy. In addition, OCTA metrics may be useful for evaluating macular ischemia in DME.
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http://dx.doi.org/10.18240/ijo.2021.12.12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8640760PMC
December 2021

Combined berberine and probiotic treatment as an effective regimen for improving postprandial hyperlipidemia in type 2 diabetes patients: a double blinded placebo controlled randomized study.

Gut Microbes 2022 Jan-Dec;14(1):2003176

Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Non-fasting lipidemia (nFL), mainly contributed by postprandial lipidemia (PL), has recently been recognized as an important cardiovascular disease (CVD) risk as fasting lipidemia (FL). PL serves as a common feature of dyslipidemia in Type 2 Diabetes (T2D), albeit effective therapies targeting on PL were limited. In this study, we aimed to evaluate whether the therapy combining probiotics (Prob) and berberine (BBR), a proven antidiabetic and hypolipidemic regimen via altering gut microbiome, could effectively reduce PL in T2D and to explore the underlying mechanism. Blood PL (120 min after taking 100 g standard carbohydrate meal) was examined in 365 participants with T2D from the Probiotics and BBR on the Efficacy and Change of Gut Microbiota in Patients with Newly Diagnosed Type 2 Diabetes (PREMOTE study), a random, placebo-controlled, and multicenter clinical trial. Prob+BBR was superior to BBR or Prob alone in improving postprandial total cholesterol (pTC) and low-density lipoprotein cholesterol (pLDLc) levels with decrement of multiple species of postprandial lipidomic metabolites after 3 months follow-up. This effect was linked to the changes of fecal level responding to BBR alone or Prob+BBR treatment. Four genes encoding long-chain acyl-CoA synthetase were identified in the genome of this strain, and transcriptionally activated by BBR. BBR treatment further decreased the concentration of FFA in the culture medium of compared to vehicle. Thus, the activation of by BBR could enhance FFA import and mobilization in and diliminish the intraluminal lipids for absorption to mediate the effect of Prob+BBR on PL. Our study confirmed that BBR and Prob () could exert a synergistic hypolipidemic effect on PL, acting as a gut lipid sink to achieve better lipidemia and CVD risk control in T2D.
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http://dx.doi.org/10.1080/19490976.2021.2003176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8726654PMC
December 2021
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