Publications by authors named "Qiling Song"

14 Publications

  • Page 1 of 1

Biomimetic hydroxyapate/polydopamine composites with good biocompatibility and efficiency for uncontrolled bleeding.

J Biomed Mater Res B Appl Biomater 2021 Apr 13. Epub 2021 Apr 13.

State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian, P.R. China.

Uncontrolled bleeding is thought to be the most deadly cause of pre-hospital, traffic, and military accidents death. However, the popular commercial hemostats can only realize the hemostasis of mild bleeding. Therefore, we developed polydopamine (PDA) composite materials (PMs), which applied hydroxyapatite as the parent body. The PMs were produced via lyophilization and functionalized with amino, phenol hydroxyls groups, which endowed hydrophobicity to materials. This ensured a high aggregation ability of blood cells to the PMs and they were tested to be as high as 300% compared with the negative control group. The clotting time was shortened to 79.7% compared with the usually used commercial hemostat (Celox) in the test of in vitro hemostasis. Through the results of PT and APTT tests, blood coagulation index test, and the analysis of intracellular Ca activation, we further understood the mechanism of the hemostasis of the materials, which explained the low blood loss and quick coagulation time of the PM hemostats in detail. Besides, the low hemolysis and cytotoxicity of the PMs suggested the good biocompatibility of the hemostats, which was further proved by the regular morphology maintained by erythrocytes in the hemolysis tests. The study of nanoscale composites led the research for the methods of hemostasis.
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http://dx.doi.org/10.1002/jbm.b.34849DOI Listing
April 2021

Characterization of a novel levopimaradiene synthase gene responsible for the biosynthesis of terpene trilactones in .

Plant Signal Behav 2021 Apr 11;16(4):1885906. Epub 2021 Feb 11.

College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei, China.

Terpene trilactones (TTLs) are the main medicinal compounds of . Levopimaradiene synthase (LPS) is the crucial enzyme that catalyzes TTLs biosynthesis in . In this study, a novel LPS gene (designated as ) was cloned from leaves. The open reading frame of gene was 2520 bp in length, encoding a predicted polypeptide of 840 amino acids. Phylogenetic analysis revealed that the GbLPS2 was highly homologous with reported LPS proteins in other plants. On the basis of the genomic DNA (gDNA) template, a 4308 bp gDNA sequence of and a 913 bp promoter sequence were amplified. -acting elements in promoter analysis indicated that could be regulated by methyl jasmonate (MeJA) and abscisic acid (ABA). Tissue-specific expression analysis revealed that was mainly expressed in roots and ovulate strobilus. MeJA treatment could significantly induce the expression level of and increase the content of TTLs. This study illustrates the structure and the tissue-specific expression pattern of and demonstrates that exogenous hormones regulated the expression of and TTL content in . Our results provide a target gene for the enhancement of TTL content in via genetic engineering.
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http://dx.doi.org/10.1080/15592324.2021.1885906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971208PMC
April 2021

Functional analysis of a novel gene () from .

Plant Signal Behav 2021 Feb 1;16(2):1850627. Epub 2020 Dec 1.

College of Art, Yangtze University , Jingzhou, Hubei, China.

Cryptochrome (CRY) is a blue light receptor that is widely distributed in animals, plants, and microorganisms. as a coding gene of cryptochrome that regulates the organism gene expression and plays an important role in organism growth and development. In this study, we identified four photolyase/cryptochrome (PHR/CRY) members from the genome of . Phylogenetic tree analysis showed that the Ginkgo PHR/CRY family members were closely related to and . We isolated a cryptochrome gene, , from and analyzed its structure and function. shared high similarity with from expression level was higher in stems and leaves and lower in roots, male strobili, female strobili. expression level fluctuated periodically within 24 h, gradually increased in the dark, and decreased under blue light. The newly germinated ginkgo seedlings were cultured under dark, white light, and blue light conditions. The blue light normally induced photomorphogenesis of ginkgo seedlings, which included hypocotyl elongation inhibition, leaf expansion inhibition, and chlorophyll formation. Treating dark-adapted ginkgo leaves with blue light could induce stomatal opening. At the same time, blue light reduced the expression level of in the process of inducing photomorphogenesis and stoma opening. Our results provide evidence that expression is affected by space, circadian cycle and light, and also proves that is related to ginkgo circadian clock, photomorphogenesis and stoma opening process.
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http://dx.doi.org/10.1080/15592324.2020.1850627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849775PMC
February 2021

Full-Length Transcriptome from Seed Provides Insight into the Transcript Variants Involved in Oil Biosynthesis.

J Agric Food Chem 2020 Dec 29;68(49):14670-14683. Epub 2020 Nov 29.

Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of Ministry of Education and the Key Laboratory of Non-Wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.

Abel., belonging to the genus of Theaceae, has been widely used as a cooking oil, lubricant, and in cosmetics. Because of complicated polyploidization and large genomes, reference genome information is still lacking. Systematic characterization of gene models based on transcriptome data is a fast and economical approach for . Pacific Biosciences single-molecule long-read isoform sequencing (Iso-Seq) and Illumina RNA-Seq combined with gas chromatography were performed for exploration of oil biosynthesis, accumulation, and comprehensive transcriptome analysis in seeds at five different developmental stages. We report the first full-length transcriptome data set of seeds comprising 40,143 deredundant high-quality isoforms. Among these isoforms, 37,982 were functionally annotated, and 271 (2.43%) belonged to fatty acid metabolism. A total of 8,344 full-length unique transcript models were obtained, and 8,151 (97.69%) of them produced more than two isoforms, suggesting a high degree of transcriptome complexity in seeds. A total of 783 alternative splicing (AS) events were identified, among which the retained intron was the most abundant. We also obtained 1,910 long noncoding RNAs (lncRNAs) and found that AS events occurred in these lncRNAs. Potential transcript variants of genes involved in oil biosynthesis were also investigated. After performing weighted correlation network analysis, we found seven "gene modules" and hub genes for each module showing a significant association with oil content. The series test of clusters classified these modules into four significant profiles based on gene expression patterns. Protein-protein interaction network analysis showed that upregulated interacted with 17 genes encoding the enzymes playing key roles in oil synthesis. and transcriptional factors also showed significant interactions with key genes involved in oil synthesis. Collectively, our data advance the knowledge of RNA isoform diversity in seeds at different developmental stages and provide a rich resource for functional studies on oil synthesis in .
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http://dx.doi.org/10.1021/acs.jafc.0c05381DOI Listing
December 2020

Efficient antibacterial dextran-montmorillonite composite sponge for rapid hemostasis with wound healing.

Int J Biol Macromol 2020 Oct 22;160:1130-1143. Epub 2020 May 22.

State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, PR China. Electronic address:

Montmorillonite (MMT) powder, as the most effective hemostats in natural silicates, is restricted for commercial application due to its embolic effect. Until now, it's still a challenge to control the leakage of MMT and avoid its side-effects. Herein, poly aldehyde dextran (PDA)/MMT composite sponge (PM) with commendable tissue adhesion, antibacterial, and wound healing performances is developed for massive hemorrhage control. Based on the high degree of perfect synergism of PDA and MMT, the PM sponge can rapidly seal the wound, and promote cells aggregation and adhesion, whole coagulation system activation, resulting in shortened clotting time from 480 s to <10 s in vitro. Therefore, PM sponge with low exothermic effects achieves hemostasis in limited time, decreasing nearly 95% blood loss in the femoral artery and vein incision in rat models. Furthermore, with the intensive tissue adhesion (~47 kPa), PM sponge not only exhibits antibacterial activity to Escherichia coli, but also succeeds in accelerating wound healing. Importantly, the low cytotoxic sponge verifies to be a little hemolytic and skin irritant hemostat. Thus, the biocompatible PM sponge may provide a new strategy for reintroduction of MMT in hemostatic fields, and a safe-effective avenue for clays to control bleeding.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.140DOI Listing
October 2020

Comparative transcriptome analysis revealing the potential mechanism of seed germination stimulated by exogenous gibberellin in Fraxinus hupehensis.

BMC Plant Biol 2019 May 15;19(1):199. Epub 2019 May 15.

College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, Hubei, China.

Background: Fraxinus hupehensis is an endangered tree species that is endemic to in China; the species has very high commercial value because of its intricate shape and potential to improve and protect the environment. Its seeds show very low germination rates in natural conditions. Preliminary experiments indicated that gibberellin (GA) effectively stimulated the seed germination of F. hupehensis. However, little is known about the physiological and molecular mechanisms underlying the effect of GA on F. hupehensis seed germination.

Results: We compared dormant seeds (CK group) and germinated seeds after treatment with water (W group) and GA (G group) in terms of seed vigor and several other physiological indicators related to germination, hormone content, and transcriptomics. Results showed that GA treatment increases seed vigor, energy requirements, and trans-Zetain (ZT) and GA contents but decreases sugar and abscisic acid (ABA) contents. A total of 116,932 unigenes were obtained from F. hupehensis transcriptome. RNA-seq analysis identified 31,856, 33,188 and 2056 differentially expressed genes (DEGs) between the W and CK groups, the G and CK groups, and the G and W groups, respectively. Up-regulation of eight selected DEGs of the glycolytic pathway accelerated the oxidative decomposition of sugar to release energy for germination. Up-regulated genes involved in ZT (two genes) and GA (one gene) biosynthesis, ABA degradation pathway (one gene), and ABA signal transduction (two genes) may contribute to seed germination. Two down-regulated genes associated with GA signal transduction were also observed in the G group. GA-regulated genes may alter hormone levels to facilitate germination. Candidate transcription factors played important roles in GA-promoted F. hupehensis seed germination, and Quantitative Real-time PCR (qRT-PCR) analysis verified the expression patterns of these genes.

Conclusion: Exogenous GA increased the germination rate, vigor, and water absorption rate of F. hupehensis seeds. Our results provide novel insights into the transcriptional regulation mechanism of effect of exogenous GA on F. hupehensis seed germination. The transcriptome data generated in this study may be used for further molecular research on this unique species.
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http://dx.doi.org/10.1186/s12870-019-1801-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521437PMC
May 2019

A highly efficient, in situ wet-adhesive dextran derivative sponge for rapid hemostasis.

Biomaterials 2019 06 16;205:23-37. Epub 2019 Mar 16.

State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian, 116024, PR China. Electronic address:

Uncontrolled hemorrhage is closely related to the high risk of death. However, local hemostats still have various defects and side effects. Herein, an aldehyde dextran (PDA) sponge with proper absorption and adhesion properties is developed for hemorrhage control. PDA sponge with pore size of ∼30-50 μm fabricated by lyophilization not only absorbs blood quickly (47.7 g/g), but also possesses strong tissue adhesion (∼100 kPa). PDA sponge with low cytotoxicity and hemolysis achieves effective hemostasis and remarkable blood loss reduction in the ear vein, femoral artery and liver injuries of rabbit models. Furthermore, the exploration of hemostatic mechanisms related to tissue, blood, plasma, cells and coagulation system indicates that PDA sponge can significantly accelerate coagulation by rapid wound block, fast cells aggregation and initiation, and high coagulation factors concentration, instead of by the coagulation cascade activation. Importantly, this hemostat exhibits excellent biodegradability and nearly no skin irritation. Overall, the biodegradable and tissue adhesive PDA sponge will be a promising quick-hemostatic dressing for uncontrollable hemorrhage.
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http://dx.doi.org/10.1016/j.biomaterials.2019.03.016DOI Listing
June 2019

Mussel-inspired degradable antibacterial polydopamine/silica nanoparticle for rapid hemostasis.

Biomaterials 2018 10 26;179:83-95. Epub 2018 Jun 26.

State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian, 116024, PR China. Electronic address:

High-performance hemostasis becomes increasingly essential in civilian and military trauma. However, available topical hemostats still exist various drawbacks and side-effects. Herein, a silica nanoparticle coated with polydopamine (PDA/SiNP) with good degradability, antibacterial performance was developed for hemorrhage control. PDA/SiNP formed a porous network via lyophilization and rendered material with phenol hydroxyls, aminos, proper hydrophobicity, promising for further cells aggregation and inducing clotting. The degradation behaviors in vitro indicated that the weight loss of PDA/SiNP could attain approximately 40% just after 24 h. All results demonstrated that clotting time of blood was shortened by nearly 150 s for PDA/SiNP compared with that of commercial Celox in vitro hemostasis. PDA/SiNP could significantly accelerate coagulation by activating the extrinsic pathway of the coagulation cascade, adhering platelets and aggregating erythrocytes. Therefore, not only the PDA/SiNP achieved adequate hemostasis with low exothermic effects, but also blood loss was remarkably reduced in the femoral artery and vein injury, liver injury models. Importantly, PDA/SiNP exhibited long-lasting inhibition of Escherichia coli even after 208 h. Also, the hemolysis of PDA/SiNP with low cytotoxicity was much lower, while erythrocytes maintained regular morphology. Thus, amorphous nanoscale PDA/SiNP provided a new avenue for design of silica hemostats and nonmetallic ion antimicrobial.
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http://dx.doi.org/10.1016/j.biomaterials.2018.06.037DOI Listing
October 2018

Characterization, Function, and Transcriptional Profiling Analysis of 3-Hydroxy-3-methylglutaryl-CoA Synthase Gene (GbHMGS1) towards Stresses and Exogenous Hormone Treatments in Ginkgo biloba.

Molecules 2017 Oct 12;22(10). Epub 2017 Oct 12.

College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China.

3-Hydroxy-3-methylglutaryl-CoA synthase (HMGS) is one of the rate-limiting enzymes in the mevalonate pathway as it catalyzes the condensation of acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA. In this study, A gene (designated as ) was cloned from for the first time. contained a 1422-bp open-reading frame encoding 474 amino acids. Comparative and bioinformatics analysis revealed that GbHMGS1 was extensively homologous to HMGSs from other plant species. Phylogenetic analysis indicated that the GbHMGS1 belonged to the plant HMGS superfamily, sharing a common evolutionary ancestor with other HMGSs, and had a further relationship with other gymnosperm species. The yeast complement assay of GbHMGS1 in -deficient strain YSC6274 demonstrated that gene encodes a functional HMGS enzyme. The recombinant protein of GbHMGS1 was successfully expressed in . The in vitro enzyme activity assay showed that the and values of GbHMGS1 were 195.4 min and 689 μM, respectively. was constitutively expressed in all tested tissues, including the roots, stems, leaves, female flowers, male flowers and fruits. The transcript accumulation for was highest in the leaves. Expression profiling analyses revealed that expression was induced by abiotic stresses (ultraviolet B and cold) and hormone treatments (salicylic acid, methyl jasmonate, and ethephon) in , indicating that gene was involved in the response to environmental stresses and plant hormones.
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http://dx.doi.org/10.3390/molecules22101706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151752PMC
October 2017

Downregulated microRNA-23b promotes BMP9-mediated osteogenesis in C2C12 myoblast cells by targeting Runx2.

Mol Med Rep 2016 Mar 27;13(3):2492-8. Epub 2016 Jan 27.

Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing 40016, P.R. China.

MicroRNAs are identified as negative regulators in gene expression through silencing gene expression at the post-transcriptional and translational levels. Bone morphogenetic protein 9 (BMP9) is the most effective in inducing osteogenesis in the BMP family, the members of which were originally identified as osteoinductive cytokines. In the current study, the role of miR‑23b in the progression of BMP9‑induced C2C12 myoblasts was investigated. The results indicated that miR‑23b was significantly downregulated in C2C12 myoblasts induced by BMP9. Overexpression of miR‑23b significantly inhibited osteogenesis in the C2C12 myoblasts. In addition, it was observed that Runx2 was negatively regulated by miR‑23b at the post‑transcriptional level, via a specific target site within the 3'UTR of Runx2. Knockdown of Runx2 promoted miR‑23b‑induced inhibition of osteogenesis in C2C12 myoblasts. The expression of Runx2 was observed to be frequently upregulated in osteoblast cell lines and inversely correlated with miR‑23b expression. Thus, the results of the present study suggest that miR‑23b inhibits BMP9‑induced C2C12 myoblast osteogenesis via targeting of the Runx2 gene, acting as a suppressor. The current study contributes to the understanding of the functions of BMP9 in ossification.
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http://dx.doi.org/10.3892/mmr.2016.4814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768947PMC
March 2016

miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells.

Int J Mol Med 2015 Dec 9;36(6):1497-506. Epub 2015 Oct 9.

Key Laboratory of Diagnostic Medicine designated by the Chinese Ministry of Education, Chongqing Medical University, Chongqing 400016, P.R. China.

Bone morphogenetic proteins (BMPs), particularly BMP9, have been shown to promote the osteogenic differentiation of murine multilineage cells (MMCs) and to promote bone formation in bone diseases; however, the mechanisms involved remain poorly understood. MicroRNAs (miRNAs or miRs) have been proven to regulate mesenchymal stem cell (MSC) differentiation. In this study, we identified a novel mechanism that unravels the functional axis of a key miRNA (miR-21) which contributes to BMP9‑induced osteogenic differentiation. We screened differentially expressed miRNAs in MMCs during BMP9‑induced osteogenic differentiation and found that miR-21 was significantly upregulated by BMP9 during the osteogenesis of MMCs. Furthermore, miR-21 was confirmed to promote the osteogenic differentiation of the MMCs by suppressing Smad7, which negatively regulates the osteogenic differentiation of MMCs. The upregulation of miR-21 may promote the osteogenic differentiation of MMCs in synergy with BMP9. The findings of our study revealed a novel function of miR-21, and suggest that the overexpression of miR-21 contributes to bone formation by promoting BMP9‑induced osteogenic differentiation. Our data may provide a molecular basis for the development of novel therapeutic strategies to treat bone diseases, such as osteoporosis and other inflammatory bone diseases.
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http://dx.doi.org/10.3892/ijmm.2015.2363DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678163PMC
December 2015

Effects of elevated ozone, carbon dioxide, and the combination of both on the grain quality of Chinese hybrid rice.

Environ Pollut 2014 Jun 6;189:9-17. Epub 2014 Mar 6.

Key Lab of Crop Genetics & Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. Electronic address:

The effects of CO2 and/or O3 elevation on rice grain quality were investigated in chamber experiments with gas fumigation performed from transplanting until maturity in 2011 and 2012. Compared with the control (current CO2 and O3 concentration), elevated CO2 caused a tendency of an increase in grain chalkiness and a decrease in mineral nutrient concentrations. In contrast, elevated O3 significantly increased grain chalkiness and the concentrations of essential nutrients, while changes in starch pasting properties indicated a trend of deterioration in the cooking and eating quality. In the combination of elevated CO2 and O3 treatment, only chalkiness degree was significantly affected. It is concluded that the O3 concentration projected for the coming few decades will have more substantial effects on grain quality of Chinese hybrid rice than the projected high CO2 concentration alone, and the combination of two gases caused fewer significant changes in grain quality than individual gas treatments.
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http://dx.doi.org/10.1016/j.envpol.2014.02.016DOI Listing
June 2014

Total steroidal alkaloids from Veratrum patulum L. Inhibit platelet aggregation, thrombi formation and decrease bleeding time in rats.

J Ethnopharmacol 2012 May 18;141(1):183-6. Epub 2012 Feb 18.

School of Pharmaceutical Science and Technology, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian City, Liaoning Province 116024, PR China.

Ethnopharmacological Relevance: Veratrum patulum L. (Liliaceae) is used as one source of the Chinese traditional drug "Lilu" which has been used in the treatment of aphasia arising from apoplexy, wind type dysentery, headache, etc. for thousands of years.

Aim Of The Study: To evaluate the antithrombotic effect of Veratrum patulum L. (Liliaceae) total steroidal alkaloids (VpA) and explore the potential mechanisms.

Materials And Methods: The antithrombotic effect of VpA was evaluated in two experimental thrombosis models in rat. The potential antithrombotic mechanisms of VpA were explored by determining the coagulation parameters and platelet aggregation induced by various agonists. Cutting-tail rat model was used to evaluate the influence of VpA on bleeding time.

Results: VpA significantly inhibit the formation of arterial and venous thrombosis and showed different inhibitory effect on the platelet aggregation induced by various agonists with the following potency order: collagen>ADP>thrombin. VpA showed no influence on the coagulation parameters in rat. But VpA decreased the cutting-tail bleeding time in rat.

Conclusion: VpA possess antithrombotic effect on venous and arterial thrombosis. The antithrombotic effect of VpA is due to its inhibition to platelet aggregation, especially induced by collagen.
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http://dx.doi.org/10.1016/j.jep.2012.02.017DOI Listing
May 2012

Preparation of yuanhuacine and relative daphne diterpene esters from Daphne genkwa and structure-activity relationship of potent inhibitory activity against DNA topoisomerase I.

Bioorg Med Chem 2006 Jun 20;14(11):3888-95. Epub 2006 Feb 20.

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Zhongshan Road 158-90, Dalian 116012, China.

Two new daphne diterpene esters Yuanhuajine (2) and Yuanhuagine (4), together with three known daphne diterpene esters yuanhuacine (1), yuanhuadine (3), and yuanhuapine (5), were isolated and identified from Daphne genkwa, a traditional Chinese medicine. Their structures were elucidated by a combination of UV, IR, MS and NMR ((1)H NMR, (13)C NMR, HSQC, and HMBC) spectra. In order to explore the structure-activity relationship, three compounds 6, 7, and 8 were prepared as three derivatives of 1. Inhibitory activities against DNA topoisomerase I (topo I) were assessed for the compounds 1-8. These compounds, except for 8, exhibited potent inhibitory activities against DNA topo I at IC(50) levels of 11.1-53.4 microM and they are new type of topo I inhibitors bearing different structures compared with the known topo I inhibitors. The agarose-gel electrophoresis experiments showed that the orthoester group of daphne diterpene esters was necessary for the inhibitory activity against DNA topo I, and the inhibition against DNA topo I is probably one of the anti-tumor mechanisms of daphne diterpene esters.
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http://dx.doi.org/10.1016/j.bmc.2006.01.055DOI Listing
June 2006