Publications by authors named "Qiaoyan Li"

25 Publications

  • Page 1 of 1

Identification of a prognostic chemoresistance-related gene signature associated with immune microenvironment in breast cancer.

Bioengineered 2021 Dec;12(1):8419-8434

Department of Pharmacy, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan, China.

Breast cancer is the most common form of cancer among women globally, and chemoresistance is a major challenge to disease treatment that is associated with a poor prognosis. This study was formulated to identify a reliable prognostic biosignature capable of predicting the survival of patients with chemoresistant breast cancer (CRBC) and evaluating the associated tumor immune microenvironment. Through a series of protein-protein interaction and weighted correlation network analyses, genes that were significantly associated with breast cancer chemoresistance were identified. Moreover, univariate Cox regression and lasso-penalized Cox regression analyses were employed to generate a prognostic model, and the prognostic utility of this model was then assessed using time-dependent receiver operating characteristic (ROC) and Kaplan-Meier survival curves. Finally, The CIBERSORT and ESTIMATE algorithms were additionally leveraged to assess relationships between the tumor immune microenvironment and patient prognostic signatures. Overall, a multigenic prognostic biosignature capable of predicting CRBC patient risk was successfully developed based on bioinformatics analysis and in vitro experiments. This biosignature was able to stratify CRBC patients into high- and low-risk subgroups. ROC curves also revealed that this biosignature achieved high diagnostic efficiency, and multivariate regression analyses indicated that this risk signature was an independent risk factor linked to CRBC patient outcomes. In addition, this signature was associated with the infiltration of the tumor microenvironment by multiple immune cell types. In conclusion, the chemoresistance-associated prognostic gene signature developed herein was able to effectively evaluate the prognosis of CRBC patients and to reflect the overall composition of the tumor immune microenvironment.
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http://dx.doi.org/10.1080/21655979.2021.1977768DOI Listing
December 2021

Insight into the enhancing activity and stability of Ce modified VO/AC during cyclic desulfurization-regeneration-denitrification.

J Hazard Mater 2021 Sep 30;424(Pt B):127397. Epub 2021 Sep 30.

State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Cyclic desulfurization-regeneration-denitrification over carbon-based catalysts is a promising technology for SO and NOx simultaneous elimination in steel industry. Regeneration is imperative to the long-term operation of the process, while the research is limited. In this work, Ce modified VO/AC catalyst (CeVOx/AC) with higher desulfurization and denitrification activity was prepared and the effect of cyclic regeneration was investigated. Results illustrated that the desulfurization and denitrification activity of CeVOx/AC gradually improved with increasing the regeneration cycles at the optimum regeneration temperature of 470 °C in N. The increasing Ce, V and oxygen vacancies, enhanced surface acidity and improved redox ability contributed to the catalytic activity of regenerated catalysts. For desulfurization, more SO transformed into HSO rather than to metal sulfates after cyclic regeneration. For denitrification, the improved redox ability accelerated the oxidation of NO to active NO, bridged nitrites and nitrates, and the enhanced acidity facilitated the NH adsorption, further generating more -NH and promoting the SCR activity of regenerated samples. The CeVOx/AC with good activity and regenerative stability shows great application potential in steel industry for the simultaneous SO and NOx removal.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127397DOI Listing
September 2021

Room-temperature growth of covalent organic frameworks as the stationary phase for open-tubular capillary electrochromatography.

Analyst 2021 Oct 25;146(21):6643-6649. Epub 2021 Oct 25.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China.

Covalent organic frameworks (COFs) are a class of porous materials with high surface area, high porosity, good stability and tunable structure that have been widely used in the separation area. In this work, we have proposed the synthesis of a novel COF composed of 4,4',4''-(1,3,5-triazine-2,4,6-triyl)trianiline (Tz) and 1,4-dihydroxyterephthalaldehyde (Da) onto the capillary inner surface for electrochromatographic separation. Fourier transform infrared (FT-IR) spectroscopy, elemental analysis (EA) and scanning electron microscopy (SEM) have facilitated the characterization of the prepared capillary columns. The COF (TzDa) modified OT-CEC column exhibited satisfactory separation selectivity towards neutral compounds (such as chlorobenzenes and alkylbenzenes), acidic and basic compounds (such as phenols and anilines), food additives (vanillin and its analogues) and small biomolecules (such as amino acids and polypeptides). Furthermore, the TzDa modified capillary was quite stable and reproducible. The relative standard deviations for retention times of the test analytes (alkylbenzenes) were as follows: for intra-day ( = 3) runs (≤1.74%), inter-day ( = 3) runs (≤2.25%) and between columns ( = 3) (≤4.83%). This new type of COF-based stationary phase has tremendous potential in separation science.
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http://dx.doi.org/10.1039/d1an01402aDOI Listing
October 2021

A lipase-based chiral stationary phase for direct chiral separation in capillary electrochromatography.

Talanta 2021 Oct 11;233:122488. Epub 2021 May 11.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, 100080, China. Electronic address:

Candida antarctica lipase B (CALB) is a natural biocatalyst with an intrinsically strong chiral environment and a high degree of enantio-selectivity, which is widely used in the separation of racemates. Here, a facile and efficient covalent immobilization approach was utilized to immobilize CALB onto the capillary inner wall as a novel chiral stationary phase to explore and broaden its application in the direct chiral separation by electrochromatography. The obtained CALB immobilized capillary column was characterized by scanning electron microscopy (SEM), fluorescence imaging and Fourier transform infrared spectroscopy (FT-IR). The enantioseparation property of the CALB immobilized capillary column was confirmed by direct chiral separation of several pairs of monoamine neurotransmitter enantiomers in OT-CEC mode. Outstanding enantioseparation performance for three types of monoamine neurotransmitter enantiomers including epinephrine, norepinephrine and phenylephrine was obtained by the CALB immobilized column. Thanks to the effectiveness of covalent bonding method and the intrinsic stability of CALB, the prepared CALB immobilized capillary columns were quite steady and reproducible. The relative standard deviations for retention times of the enantiomers were as follows: for intra-day (n = 5) runs (≤0.25%), inter-day (n = 3) runs (≤0.72%) and between-columns (n = 3) (≤2.42%). After 90 consecutive runs in CEC mode, the CALB immobilized column still exhibited desirable enantionseparation performance.
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http://dx.doi.org/10.1016/j.talanta.2021.122488DOI Listing
October 2021

MiR-142-3p ameliorates high glucose-induced renal tubular epithelial cell injury by targeting BOD1.

Clin Exp Nephrol 2021 Nov 18;25(11):1182-1192. Epub 2021 Jun 18.

Department of Pharmacy, Henan Provincial People's Hospital (Zhengzhou University People's Hospital), No. 7 Weiwu Road, Zhengzhou, 450003, Henan, China.

Background: Tubular injury plays a crucial role in the pathogenesis of diabetic nephropathy (DN). It is well known that many microRNAs (miRNAs) exert crucial effects on tubular injury. This study intends to explore the effect of miR-142-3p on the apoptosis and oxidative stress of high glucose (HG)-treated renal tubular epithelial cells (HK-2) and its underlying mechanism.

Materials And Methods: HK-2 cells were exposed to HG to mimic cell injury. MTT assays and flow cytometry analyses were conducted to measure cell viability and cell apoptosis, respectively. RT-qPCR and western blot analyses were carried out to detect RNA and protein levels, respectively. The levels of oxidative stress markers were evaluated by ELISA. The binding between miR-142-3p and biorientation of chromosomes in cell division 1 (BOD1) was validated by a luciferase reporter assay.

Result: MiR-142-3p is low-expressed in HG-stimulated HK-2 cells. Functionally, miR-142-3p overexpression attenuates the apoptosis and oxidative stress of HG-stimulated HK-2 cells. Mechanistically, BOD1 was confirmed to be targeted by miR-142-3p in HK-2 cells. Moreover, BOD1 overexpression reversed the suppressive effect of miR-142-3p overexpression on the apoptosis and oxidative stress of HK-2 cells treated with HG.

Conclusion: MiR-142-3p ameliorates HG-induced renal tubular epithelial cell injury by targeting BOD1. The finding might provide novel insight into the role of miR-142-3p/BOD1 axis in DN treatment.
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http://dx.doi.org/10.1007/s10157-021-02102-yDOI Listing
November 2021

Managing Defects Density and Interfacial Strain via Underlayer Engineering for Inverted CsPbI Br Perovskite Solar Cells with All-Layer Dopant-Free.

Small 2021 Jul 12;17(28):e2101902. Epub 2021 Jun 12.

Soochow Institute for Energy and Materials Innovation (SIEMIS), School of Energy, Soochow University, Suzhou, 215006, China.

Inorganic perovskite CsPbI Br has advantages of excellent thermal stability and reasonable bandgap, which make it suitable for top layer of tandem solar cells. Nevertheless, solution-processed all-inorganic perovskites generally suffer from high-density defects as well as significant tensile strain near underlayer/perovskite interface, both leading to compromised device efficiency and stability. In this work, the defect density as well as interfacial tensile strain in inverted CsPbI Br perovskite solar cells (PeSCs) is remarkably reduced by using a bilayer underlayer composed of dopant-free 2,2',7,7'-tetrakis(N,N-dip-methoxyphenylamine)-9,9'-spirobifluorene (Spiro-OMeTAD) and copper phthalocyanine 3,4',4″,4'″-tetrasulfonated acid tetrasodium salt (TS-CuPc) nanoparticles. As compared to control devices with pristine Spiro-OMeTAD, devices based on Spiro-OMeTAD/TS-CuPc exhibit remarkably improved photovoltaic performance and enhanced thermal/humidity stability due to the better perovskite crystallization, improved interfacial passivation, and hole-collection as well as efficient interfacial strain release. As a result, a champion efficiency of 14.85% can be achieved, which is approaching to the best reported for dopant-free and inverted all-inorganic PeSCs. The work thus provides an efficient strategy to simultaneously regulate the defects density and strain issue related to inorganic perovskites.
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http://dx.doi.org/10.1002/smll.202101902DOI Listing
July 2021

In situ room-temperature preparation of a covalent organic framework as stationary phase for high-efficiency capillary electrochromatographic separation.

J Chromatogr A 2021 Jul 11;1649:462239. Epub 2021 May 11.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 100080, China. Electronic address:

Covalent organic frameworks (COFs), considered as a series of newly emerging porous organic materials, have been widely utilized in separation fields. Herein, a novel COF (TFPB-BD) was first employed as stationary phase for high-efficiency capillary electrochromatographic separation. Benzidine (BD) and 1,3,5-Tris-(4-formylphenyl)benzene (TFPB) were selected as organic linkers and then introduced into the aldehyde group modified capillary for the in situ growth of TFPB-BD onto the capillary inner wall at room temperature. The morphology and formation of TFPB coated capillary column were confirmed by a variety of tools including Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS). It's interesting that the TFPB globular crystals with nanoscale were uniformly and densely modified on the capillary inner surface. Hence, the prepared column exhibited prominent separation performance for the test analytes including alkylbenzenes, chlorobenzenes and phenolic compounds with high efficiency and high resolution. The maximum column efficiency can reach about 1.8 × 10 plates•m. Additionally, the high resolutions of anilines, amino acids and parabens were also achieved on the TFPB modified capillary. The precisions (RSDs) of the retention times of alkylbenzenes of intra-day runs (n = 3), inter-day runs (n = 3) and parallel columns (n = 3) were all less than 2.83%. This innovative COF-based stationary phase gives great promise for the chromatographic separation field.
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http://dx.doi.org/10.1016/j.chroma.2021.462239DOI Listing
July 2021

Benzoic acid-modified monolithic column for separation of hydrophilic compounds by capillary electrochromatography with high content of water in mobile phase.

J Chromatogr A 2021 Jun 22;1647:462166. Epub 2021 Apr 22.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 100080, China. Electronic address:

Hydrophilic column combined with mobile phase containing high content of water is a green method for the separation of polar compounds, but there are few related studies, and the separation efficiency and performance of existing columns still needs to be improved. In this work, a novel monolithic column for separation of hydrophilic compounds under both high water content and HILIC condition, was prepared by in-situ polymerization using 4-vinylbenzoic acid (VBA) and 1-(Acryloyloxy)-3-(methacryloyloxy)-2-propanol (AMAP) as functional monomers. The poly(VBA-co-AMAP) monolithic column showed good separation performance towards various polar compounds under different chromatographic conditions based on the π-interaction, hydrophobic and hydrogen bonding interactions provided by 4-vinylbenzoic acid functional monomer. The highest column efficiency for adenine was over 2.15 × 10 plates m (theoretical plate, N). In addition, the monolith showed good stability and reproducibility, the relative standard deviations (RSDs) of retention times within days (n = 5), between days (n = 5), between columns (n = 3) and between batches (n = 3) were 0.47-1.13%, 1.20-2.68%, 0.59-1.78% and 1.54-3.60%, respectively. This novel type of monolith has great application potential in the separation of hydrophilic compounds.
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http://dx.doi.org/10.1016/j.chroma.2021.462166DOI Listing
June 2021

Facile preparation of ethanediamine-β-cyclodextrin modified capillary column for electrochromatographic enantioseparation of Dansyl amino acids.

J Chromatogr A 2021 Apr 18;1643:462082. Epub 2021 Mar 18.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 10080, China. Electronic address:

Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed. A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary. The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS). This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes. The EDA-β-CD modified column showed eminent enantioseparation performance towards five Dansyl-DL-amino acids (the DL-forms of valine, threonine, leucine, phenylalanine, serine). Besides, the prepared columns were perfectly reproducible and stable. The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3) are below 0.54%, 1.35% and 4.89%, individually. This innovative chiral stationary phase shows a broader application view and scope in chiral recognition domain.
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http://dx.doi.org/10.1016/j.chroma.2021.462082DOI Listing
April 2021

Glycine-modified organic polymer monolith featuring zwitterionic functionalities for hydrophilic capillary electrochromatography.

J Chromatogr A 2020 Oct 19;1629:461497. Epub 2020 Aug 19.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 10080, China. Electronic address:

Allylglycine, a conventional amino acid derivative, possesses typical zwitterionic and hydrophilic functionalities deriving from the carboxyl and amino groups in its structure. A novel monolithic column poly(allylglycine-co-1, 3, 5-triacryloylhexahydro-1, 3, 5-triazine) (AGly-co-TAT) with powerful hydrophilic selectivity and obvious zwitterionic feature was synthesized successfully with the monomer allyglycine and the cross-linker 1, 3, 5-triacryloylhexahydro-1, 3, 5-triazine through in-situ copolymerization for capillary electrochromatography. The obtained monolithic column has good permeability. Due to the zwitterionic functional groups of allylglycine, the poly(AGly-co-TAT) monolithic column can generate a cathodic and anodic electroosmotic flow (EOF) by changing the mobile phase pH, which is beneficial to expand its application range. The separations of different series of polar analytes, thioureas, xanthines, phenols, peptides and acidic compounds are achieved on this hydrophilic monolithic column due to the powerful hydrophilic, electrostatic and hydrogen bond interactions. Using this monolithic column, hydrophilic separations are achieved even at a lower level of 50% organic solvent. The separation efficiency up to 1.41 × 10 N m and 1.19 × 10 N m is achieved for the separation of theophylline and phenol, respectively. For a real sample, cytochrome C digestion, the monolithic column shows good separation performance, which offers the potential application of the monolithic column on proteomics study.
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http://dx.doi.org/10.1016/j.chroma.2020.461497DOI Listing
October 2020

Fluoro-functionalized stationary phases for electrochromatographic separation of organic fluorides.

J Chromatogr A 2020 Aug 28;1625:461269. Epub 2020 May 28.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 10080, China. Electronic address:

Fluorous affinity means remarkably specific interaction between highly organic fluorides. This work aims to explore the potential of fluoro-functionalized stationary phase for the separation of organic fluorides by means of fluorous-fluorous interaction. Here, by using the Michael addition strategy between 1H,1H,2H,2H-perfluorodecanethiol (PFDT) and polydopamine (PD), a novel fluoro-functionalized stationary phase was synthesized for open-tubular capillary electrochromatography (OT-CEC). The [email protected] was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray Photoelectron Spectrometer (XPS). The [email protected]@capillary exhibited outstanding separation performance towards neutral compounds (such as alkylbenzenes and chlorobenzenes) and organic fluorides (such as fluorobenzenes and perfluoroalkyl methacrylates etc.) with high resolution and high separation efficiency by hydrophobic interaction and fluorous-fluorous interaction. In addition, the column shows good stability and reproducibility. The relative standard deviations (RSDs) of the retention time for intra-day (n = 5) and inter-day (n = 3) runs and between columns (n = 3) are less than 0.39%, 1.22% and 3.87%, respectively. This novel type of fluoro-functionalized stationary phase represents a great application potential in organic fluorides separation field.
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http://dx.doi.org/10.1016/j.chroma.2020.461269DOI Listing
August 2020

A new insight into the promotional effect of nitrogen-doping in activated carbon for selective catalytic reduction of NO with NH.

Sci Total Environ 2020 Oct 12;740:140158. Epub 2020 Jun 12.

State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, PR China. Electronic address:

A series of N-doped carbons were prepared to investigate the effect of different N-containing groups on selective catalytic reduction (SCR) of NOx with NH. Combined the SCR activity with the results of porosity analysis and X-ray photoelectron spectroscopy, it's deduced that the pyridinic N (N-6) rather than the surface area or doped total N was mainly responsible for the promoted SCR activity. The electron paramagnetic resonance and O-temperature programmed desorption (O-TPD) experiments indicated that N-6 created numerous of oxygen vacancy. The NO+O-TPD and transient response of NH further demonstrated that the increased oxygen vacancy enhanced the absorbability and reactivity of NOx, therefore the SCR reaction was elevated by accelerating the reaction in the Langmuir-Hinshelwood (L-H) mechanism. Furthermore, the NH-TPD suggested that N-6 was conductive to the NH adsorption. In situ DRIFTs of NH adsorption and reaction illustrated that the increased NH mainly existed as NH species, which were quickly consumed by NO+O, further elevated the reaction between gaseous NO and adsorbed NH in the Eley-Rideal (E-R) mechanism. The N-6 groups doped in the activated carbons facilitated the L-H and E-R reactions and thus promoted the SCR activity.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140158DOI Listing
October 2020

Differences in Gut Microbial Diversity are Driven by Drug Use and Drug Cessation by Either Compulsory Detention or Methadone Maintenance Treatment.

Microorganisms 2020 Mar 13;8(3). Epub 2020 Mar 13.

School of Public Health, Lanzhou University, No. 222 TianshuiNanlu, Lanzhou 730000, China.

In this work, we investigate differences in gut microbial diversity driven by drug use or by the widely used methods for drug cessation: methadone maintenance treatment (MMT) and compulsory detention (CD). Methods: 99 participants (28 CD participants, 16 MMT patients, 27 drug users, and 28 healthy controls) were selected using strict inclusion criteria. Nutritional intake and gut microbial diversity were analyzed with bioinformatics tools and SPSS 20.0. Results: Alpha diversity was not significantly different among groups, whereas beta diversity of gut microbiota and nutrient intake were significantly higher among MMT patients. Taxa were unevenly distributed between groups, with drug users having the highest proportion of and MMT patients having the highest abundance of and Conclusion: Drug use, cessation method, and diet contribute to shaping human gut communities. High beta diversity among MMT patients is likely driven by methadone use and high nutrient intake, leading to increased orexin A and enrichment for beneficial bacteria, while diversity in CD participants is largely influenced by diet.
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http://dx.doi.org/10.3390/microorganisms8030411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143234PMC
March 2020

Changes in levels of enzymes and osmotic adjustment compounds in key species and their relevance to vegetation succession in abandoned croplands of a semiarid sandy region.

Ecol Evol 2020 Feb 3;10(4):2269-2280. Epub 2020 Feb 3.

Key Laboratory of Resource Plants West China Subalpine Botanical Garden Institute of Botany Chinese Academy of Sciences Beijing China.

Reclamation of cropland from grassland is regarded as a main reason for grassland degradation; understanding succession from abandoned cropland to grassland is thus crucial for vegetation restoration in arid and semiarid areas. Soil becomes dry when cropland is reverted to grassland, and enzyme and osmotic adjustment compounds may help plants to adapt to a drying environment. Croplands that were abandoned in various years on the Ordos Plateau in China, were selected for the analysis of the dynamics of enzymes and osmotic adjustment compounds in plant species during vegetation succession. With increasing number of years since abandonment, levels of superoxide dismutase increased in , first decreased and then increased in and , and fluctuated in . Levels of peroxidase and catalase in the four species fluctuated; levels of proline, soluble sugar, and soluble protein either decreased or first increased and then generally decreased. According to a drought resistance index, the drought resistance of the four species was ranked in descending order as follows:  >  >  > . The drought resistance ability of the different species was closely linked with vegetation succession from communities dominated by annual and biennial species (with main accompanying species of and ) to communities dominated by perennial species ( and ) when soil became dry owing to increasing evapotranspiration after cropland abandonment. The restoration of steppe after cropland abandonment on the Ordos Plateau is recommended both as high-quality forage and for environmental sustainability.
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http://dx.doi.org/10.1002/ece3.6067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042792PMC
February 2020

Visible-light-mediated photocatalytic cross-coupling of acetenyl ketones with benzyl trifluoroborate.

Org Biomol Chem 2020 02 21;18(6):1073-1077. Epub 2020 Jan 21.

Department of Pharmacy, Henan Provincial People's Hospital; Zhengzhou University People's Hospital, Zhengzhou, Henan, 450003, P. R. China.

In this report, we describe a simple visible light-triggered Barbier-type reaction by employing acetenyl ketones with benzyl trifluoroborates. Through a radical-radical cross-coupling process, this photocatalytic protocol furnished a wide range of tertiary propargyl alcohols. Mechanistic investigation indicated that proton-coupled electron transfer (PCET) might be involved in the photochemical transformations.
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http://dx.doi.org/10.1039/c9ob02624jDOI Listing
February 2020

Coproduction of hydrogen and methane in a CSTR-IC two-stage anaerobic digestion system from molasses wastewater.

Water Sci Technol 2019 Jan;79(2):270-277

School of Forestry, Northeast Forestry University, Harbin, 150040, China E-mail:

A continuous hydrogen and methane production system in a two-stage process has been investigated to increase energy recovery rate from molasses wastewater in this study. This system consisted of a continuous stirred-tank reactor for hydrogen production and an internal circulation (IC) reactor for methane production, and was studied under the influent organic loading rate (OLR) of 18, 24, 30 and 36kg COD/(m·d) (COD: chemical oxygen demand). The maximum volumetric hydrogen production rate of 2.41 L/(L·d) was obtained at the OLR of 30kg COD/(m·d) with a hydrogen content of 42%, and the maximum volumetric methane production rate of 2.4 L/(L·d) with a methane content of 74.45% was obtained at the OLR of 36kg COD/(m·d) using the effluents of hydrogen fermentation as substrate. The maximum of 71.06% of the molasses wastewater energy was converted to biogas (hydrogen and methane) at the OLR of 30kg COD/(m·d).
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http://dx.doi.org/10.2166/wst.2019.042DOI Listing
January 2019

Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR-125b with MCL-1 by regulating mitochondrial apoptosis pathways in ovarian cancer.

Cancer Med 2018 09 20;7(9):4530-4541. Epub 2018 Jul 20.

Department of Pathology, Xiangya Hospital, Central South University, Changsha, China.

Recently, an increasing number of studies have focused on the key function of long noncoding RNAs (lncRNAs) in biological activity. Abnormal lncRNA expression was found to relate to the development and pathogenesis of multiple cancers. LncRNA LINC00152 served as an oncogene in multiple cancers; however, its role in ovarian cancer remains unknown. In our research study, LINC00152 was upregulated in ovarian cancer tissues and cell lines. An increasing LINC00152 level was positively correlated with the histological grade, clinical stage, and poor prognosis of ovarian cancer patients. In addition, knockdown of LINC00152 reduced cell growth, induced cell apoptosis, and suppressed tumor growth. Moreover, we revealed that LINC00152 and Myeloid cell leukemia-1 (MCL-1) were targeted by miR-125b and had the same miR-125b combining site. The miR-125b level was negatively correlated with the expression of LINC00152, while MCL-1 was positively related to the LINC00152 level. MiR-125b could affect LINC00152 levels as evaluated by qRT-PCR. Finally, we affirmed that LINC00152 mediated cell proliferation by affecting MCL-1 expression and MCL-1-mediated mitochondrial apoptosis pathways and by working as a competitive endogenous RNA (ceRNA) of miR-125b. In summary, based on ceRNA theory, the combined research on miR-125b and MCL-1, and taking LINC00152 as a new study point, we provide new insight into the molecular mechanism of reversing cell proliferation in ovarian cancer.
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http://dx.doi.org/10.1002/cam4.1547DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6144155PMC
September 2018

Tree-ring widths are good proxies of annual variation in forest productivity in temperate forests.

Sci Rep 2017 05 16;7(1):1945. Epub 2017 May 16.

Key Laboratory for Forest Resources & Ecosystem Processes of Beijing, and College of Forestry, Beijing Forestry University, Beijing, 100083, China.

Tree rings have long been used to calibrate the net primary production (NPP) time-series predicted by process-based models, based on an implicit assumption that ring-width indices (RWI) can well reflect temporal NPP change. However, this assumption has seldom been tested systematically. In this study, 36 plots were set in three forest types from four sites along a latitudinal gradient in northeast China. For each plot, we constructed chronologies and stand NPP of the past 20 years to examine: is RWI a good proxy of inter-annual variation of forest NPP for different forest types under different climate? If it is, why? Our results indicate that RWI was closely related to stand NPP in most cases, and could be used as a good proxy of NPP in temperate forests. Standard and arstan chronologies were better related to NPP series than residual chronology. Stand NPP time-series were mainly determined by large trees, and the correlation between RWI and NPP was also higher for larger trees. We suggest that large trees and dominant species of canopy layer should be sampled for chronology construction. Large trees are major contributors of forest biomass and productivity, and should have priority in forest conservation in a rapid-warming world.
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http://dx.doi.org/10.1038/s41598-017-02022-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5434002PMC
May 2017

Effects of biophysical constraints, climate and phylogeny on forest shrub allometries along an altitudinal gradient in Northeast China.

Sci Rep 2017 03 7;7:43769. Epub 2017 Mar 7.

College of Forestry, Beijing Forestry University, Beijing 100083, China.

Whether there is a general allometry law across plant species with different sizes and under different environment has long been controversial and shrubs are particularly useful to examine these questions. Here we sampled 939 individuals from 50 forest shrub species along a large altitudinal gradient. We tested several allometry models with four relationships simultaneously (between stem diameter, height, leaf, stem and aboveground biomass), including geometric, elastic and stress similarity, and metabolic scaling theory's predictions on small plants (MST) and trees (MST). We also tested if allometric exponents change markedly with climate and phylogeny. The predicted exponents of MST, elastic similarity and stress similarity (models for trees) were not supported by our data, while MST and geometric similarity gained more support, suggesting the finite size effect is more important for shrub allometries than being a woody plant. The influence of climate and phylogeny on allometric exponents were not significant or very weak, again suggesting strong biophysical constraints on shrub allometries. Our results reveal clear differences of shrub allometries from previous findings on trees (e.g. much weaker climatic and phylogenic control). Comparisons of herbs, shrubs and trees along a same climatic gradient are needed for better understanding of plant allometries.
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http://dx.doi.org/10.1038/srep43769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5339776PMC
March 2017

Effects of cytochrome P450 3A4 and non-genetic factors on initial voriconazole serum trough concentrations in hematological patients with different cytochrome P450 2C19 genotypes.

Xenobiotica 2017 Dec 3;47(12):1121-1129. Epub 2017 Jan 3.

a Department of Pharmacy and.

1. Polymorphisms of cytochrome P450 2C19 (CYP2C19) is an important factor contributing to variability of voriconazole pharmacokinetics. Polymorphisms of CYP3A4, CYP3A5, CYP2C9 and non-genetic factors such as age, gender, body mass index (BMI), transaminase levels, concomitant medications might also affect voriconazole initial steady serum trough concentration (VIC) in haematological patients, but the effects were not clear. 2. Eighteen single-nucleotide polymorphisms in CYP2C19, CYP3A4, CYP3A5, CYP2C9 were genotyped. Patients were stratified into two groups according to CYP2C19 genotype. Group 1 were patients with CYP2C19*2 or CYP2C19*3, and Group 2 were homozygous extensive metabolizers. The effects were studied in different groups. VIC was adjusted on daily dose (VIC/D) for overcoming effect of dose. 3. A total of 106 blood samples from 86 patients were included. In final optimal scaling regression models, polymorphisms of rs4646437 (CYP3A4), age, BMI was identified to be factors of VIC/D in Group 1 (R=.255, p < .001). Only age was confirmed as a factor of VIC/D in Group 2 (R=0.144, p = .021). 4. Besides polymorphisms of CYP2C19, in individualized medication of voriconazole in haematological patients, polymorphisms of CYP3A4, and non-genetic factors as BMI, age should also be taken into account, especially for individuals with CYP2C19*2 or CYP2C19*3.
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http://dx.doi.org/10.1080/00498254.2016.1271960DOI Listing
December 2017

Upregulation of p27Kip1 by demethylation sensitizes cisplatin-resistant human ovarian cancer SKOV3 cells.

Mol Med Rep 2016 Aug 14;14(2):1659-66. Epub 2016 Jun 14.

Department of Gynecology and Obstetrics, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.

Ovarian cancer has a poor prognosis due to its chemoresistance, and p27Kip1 (p27) has been implicated in tumor prognosis and drug-resistance. However, the regulatory mechanisms of p27 in drug‑resistance in ovarian cancer remain unknown. The current study successfully established chemoresistant cell lines using paclitaxel (TAX), cisplatin (DDP) and carboplatin (CBP) in SKOV3 ovarian cancer cells. The results indicated that the expression levels of p27 were dramatically downregulated in chemoresistant cells. However, 5-aza-2'-deoxycytidine (5-aza) treatment restored p27 expression in DDP-resistant cells, and increased their sensitivity to DDP. In addition, it was observed that the methylation of DDP‑resistant cells, which was downregulated by 5‑aza treatment, was significantly higher compared with SKOV3 cells. Additionally, the overexpression of p27 arrested the cell cycle in S phase and promoted an apoptotic response to DDP. In conclusion, p27 was involved in chemoresistance of SKOV3 cells. Upregulated p27 expression induced by demethylation may enhance sensitivity to DDP through the regulation of the cell cycle.
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http://dx.doi.org/10.3892/mmr.2016.5399DOI Listing
August 2016

[Jaridonin induces apoptosis in human esophageal cancer cells by depleting GSH and inducing DNA damage].

Zhonghua Zhong Liu Za Zhi 2015 Jan;37(1):11-7

Clinical Pharmacology Laboratory, Henan Province People's Hospital, Zhengzhou 450003, China. Email:

Objective: The aim of this study was to explore the molecular mechanism of apoptosis in esophageal cancer cells induced by Isodon rubescens.

Methods: The DNA-damage effect of Jaridonin was detected by single cell gel electrophoresis (SCGE). The p53 protein was determined by Western blot. GSH assay kit was employed to determine the GSH content in human esophageal cancer EC-1 cells. Intracellular levels of hydrogen peroxide (H2O2) or superoxide (O(2).-) were determined using the redox-sensitive probes 2', 7'-dichlorodihydrofluorescein diacetate (DCF) or dihydroethidium (DHE), and the fluorescence signal was assayed by fluorescence microscopy and by flow cytometry.

Results: Jaridonin induced DNA damage in EC-1 cells remarkably. The olive tail moments (OTM) of control and 20, 40 µmol/L Jaridonin were 3.2, 45.2 and 89.0, respectively. Compared with the control, the differences were significant (P < 0.01 for both). Jaridonin resulted in extensive p53 up-regulation in the EC-1 cells. More importantly, the p53 up-regulation occurred as early as 2 h after Jaridonin incubation, and in a time-dependent manner (P < 0.05). p53 siRNA transfection inhibited apoptosis in the EC-1 cells, and the Jaridonin-induced apoptosis rate was reduced from 38.5% to 8.8%. Intracellular level of H2O2 was increased by Jaridonin, whereas the level of O(2).- was barely changed. The GSH content in EC-1 cells was reduced from (10.3 ± 1.6) nmol/mg protein to (4.6 ± 2.1) nmol/mg protein after 20 µmol/L Jaridonin incubation for 8 h, and it was further reduced with the increase of Jaridonin concentration. Jaridonin induced DNA damage, H2O2 accumulation and apoptosis were significantly attenuated in the presence of GSH, but Jaridonin showed little effect on normal human liver L-02 cells.

Conclusions: Jaridonin selectively induces apoptosis in esophageal cancer EC-1 cells through H2O2-mediated DNA damage by depleting GSH.
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January 2015

miR-7 and miR-218 epigenetically control tumor suppressor genes RASSF1A and Claudin-6 by targeting HoxB3 in breast cancer.

Biochem Biophys Res Commun 2012 Jul 14;424(1):28-33. Epub 2012 Jun 14.

Department of Gynecology and Obstetrics, Second Xiangya Hospital of Central South University, 139 Middle Renmin Road, Changsha, Hunan 410011, China.

Many microRNAs have been implicated as key regulators of cellular growth and differentiation and have been found to dysregulate proliferation in human tumors, including breast cancer. Cancer-linked microRNAs also alter the epigenetic landscape by way of DNA methylation and post-translational modifications of histones. Aberrations in Hox gene expression are important for oncogene or tumor suppressor during abnormal development and malignancy. Although recent studies suggest that HoxB3 is critical in breast cancer, the putative role(s) of microRNAs impinging on HoxB3 is not yet fully understood. In this study, we found that the expression levels of miR-7 and miR-218 were strongly and reversely associated with HoxB3 expression. Stable overexpression of miR-7 and miR-218 was accompanied by reactivation of tumor suppressor genes including RASSF1A and Claudin-6 by means of epigenetic switches in DNA methylation and histone modification, giving rise to inhibition of the cell cycle and clone formation of breast cancer cells. The current study provides a novel link between overexpression of collinear Hox genes and multiple microRNAs in human breast malignancy.
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http://dx.doi.org/10.1016/j.bbrc.2012.06.028DOI Listing
July 2012

Expression of the hepatocyte growth factor and c-Met in colon cancer: correlation with clinicopathological features and overall survival.

Tumori 2012 Jan-Feb;98(1):105-12

Department of Surgery, Capital Institute of Pediatrics, Peking Union Medical College, Beijing, People’s Republic of China.

Aim And Background: The hepatocyte growth factor (HGF)/c-Met signaling system has been implicated in the development and progression of colon cancer, but the relationship between the expression of HGF or c-MET and clinicopathologic features remains controversial. In the study, we analyzed the expression of HGF and c-Met in colon cancer and assessed the influence of the expression of this growth factor and its receptor on clinical and histological parameters and patient survival.

Methods And Study Design: We investigated the mRNA expression of HGF and c-Met with real-time PCR in 90 unselected colon carcinomas and the corresponding normal mucosa. Furthermore, HGF and c-Met protein expression was investigated with immunohistochemistry in all the samples.

Results: The mRNA and protein expression levels of HGF and c-Met were significantly higher in colon cancer than in matched normal mucosa. The protein level in most of the cases investigated was correlated with the mRNA level. Overexpression of HGF and c-Met, at both protein and mRNA levels, was correlated with depth of invasion, lymph node metastases and overall AJCC stage. According to univariate analysis, the mean survival time was shorter in the HGF-positive and c-Met-positive groups. Multivariate Cox analysis showed that high M stage and the expression of c-Met independently had a negative impact on overall survival.

Conclusions: The HGF/c-Met signaling pathway may be involved in the pathogenesis and progression of colon cancer. C-Met overexpression can be used as a useful parameter to evaluate the prognosis of colon cancer.
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http://dx.doi.org/10.1700/1053.11508DOI Listing
July 2012

Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells.

Virol J 2010 Oct 14;7:270. Epub 2010 Oct 14.

Guangzhou Center for Disease Control and Prevention, 23 Zhongshan 3rd Road, Guangzhou, Guangdong, China.

Background: Dengue viruses (DENs) are the wildest transmitted mosquito-borne pathogens throughout tropical and sub-tropical regions worldwide. Infection with DENs can cause severe flu-like illness and potentially fatal hemorrhagic fever. Although RNA interference triggered by long-length dsRNA was considered a potent antiviral pathway in the mosquito, only limited studies of the value of small interfering RNA (siRNA) have been conducted.

Results: A 21 nt siRNA targeting the membrane glycoprotein precursor gene of DEN-1 was synthesized and transfected into mosquito C6/36 cells followed by challenge with DEN. The stability of the siRNA in cells was monitored by flow cytometry. The antiviral effect of siRNA was evaluated by measurement of cell survival rate using the MTT method and viral RNA was quantitated with real-time RT-PCR. The presence of cells containing siRNA at 0.25, 1, 3, 5, 7 days after transfection were 66.0%, 52.1%, 32.0%, 13.5% and 8.9%, respectively. After 7 days incubation with DEN, there was reduced cytopathic effect, increased cell survival rate (76.9 ± 4.5% vs 23.6 ± 14.6%) and reduced viral RNA copies (Ct value 19.91 ± 0.63 vs 14.56 ± 0.39) detected in transfected C6/36 cells.

Conclusions: Our data showed that synthetic siRNA against the DEN-1 membrane glycoprotein precursor gene effectively inhibited DEN-1 viral RNA replication and increased C6/36 cell survival rate. siRNA may offer a potential new strategy for prevention and treatment of DEN infection.
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http://dx.doi.org/10.1186/1743-422X-7-270DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965154PMC
October 2010
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