Publications by authors named "Qianyu Zhao"

20 Publications

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A study on the characteristics of cognitive function in patients with multiple system atrophy in China.

Sci Rep 2021 Mar 2;11(1):4995. Epub 2021 Mar 2.

Department of Neurology, Dongfang Hospital, Beijing University of Chinese Medicine, Fengtai District, Beijing, 100078, China.

Nonmotor symptoms in patients with multiple system atrophy (MSA) have received an increasing amount of attention in recent years, but no research on MSA patients' cognitive characteristics has been conducted in China. To evaluate the cognitive function of MSA patients in China. Using a case-control study design, 256 MSA patients and 64 controls were evaluated by the Montreal cognitive assessment (MoCA) scale to characterize their cognitive function. Like the controls, 60.5% of the patients with MSA had cognitive impairment, but the characteristics of cognitive impairment between the two groups were different. The cognitive impairment in MSA patients was prominent in the cognitive domains of visuospatial/executive functions, naming, attention, and orientation; particularly, the visuospatial/executive functions were the most significantly impaired, while impairment in language function was mainly seen in the controls. Besides, impairments in visuospatial/executive functions, attention, language, and orientation were more prominent in MSA-P (MSA with predominant Parkinsonism) patients than in MSA-C (MSA with predominant cerebellar ataxia). The cognitive impairments were more severe in patients with probable MSA than in patients with possible MSA. In addition, the results showed that the level of cognitive function was negatively correlated with the severity of MSA. This study, which characterized the cognitive function of MSA patients with the largest sample size known so far in China, found that patients with MSA do have cognitive impairment and display specific characteristics. Therefore, the cognitive impairment of MSA should be paid more attention.The study has been registered in the Chinese Clinical Trial Registry (ChiCTR) (Registration No: ChiCTR1900022462).
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http://dx.doi.org/10.1038/s41598-021-84393-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925668PMC
March 2021

Breast milk EPA associated with infant distractibility when EPA level is low.

Nutrition 2021 06 11;86:111143. Epub 2021 Jan 11.

Chinese Academy of Sciences Key Laboratory of Nutrition, Metabolism, and Food Safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China. Electronic address:

Objectives: Dietary ω-3 polyunsaturated fatty acids (ω-3 PUFAs) may affect infants' executive function (EF), although it remains unclear whether this may be the effect of total ω-3 PUFAs or any specific ω-3 PUFA. We assessed the associations between ω-3 PUFAs in breast milk and EF in infants at 8 mo of age.

Methods: Milk samples from the mothers of 120 breast-fed infants were collected at 42 d and 8 mo postpartum in Beijing, China. Infant's EF was evaluated by planning tasks and A-not-B tasks, including working memory, distractibility, and inhibition of prepotent response at age 8 mo.

Results: Eicosapentaenoic acid (EPA) concentrations in breast milk were significantly higher at 42 d than 8 mo postpartum. Breast milk EPA levels at both 42 d (P = 0.037) and 8 mo (P = 0.005) postpartum were negatively associated with infant distractibility when EPA levels were low (< 0.05%). No significant association was observed for other ω-3 PUFAs with infant EF scores.

Conclusions: Our results suggest a beneficial effect of higher EPA in breast milk (improving infant's attention) when its levels are below a certain threshold.
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http://dx.doi.org/10.1016/j.nut.2021.111143DOI Listing
June 2021

Synthesis of magnetic covalent organic framework molecularly imprinted polymers at room temperature: A novel imprinted strategy for thermo-sensitive substance.

Talanta 2021 Apr 13;225:121958. Epub 2020 Dec 13.

School of Chemistry and Chemical Engineering, Harbin Institute of Technology, No.92, West Dazhi Street, Nangang District, Harbin, Heilongjiang, 150001, China. Electronic address:

Molecularly imprinted polymers (MIPs) with specific selective recognition have shown excellent performance in the rapid and efficient separation and enrichment of targets in complex systems. Unfortunately, it is not suitable for thermosensitive substances with biological functions. To this end, an imine-linked MIPs with covalent organic frameworks and magnetic nanoparticles was developed by using a room temperature synthesis strategy for the purification of Cyaninin-3-O-glucoside (C3G) from black chokeberry. The prepared material recognized C3G through π-π interaction, assisted by hydrogen bond, and will not be disturbed by water environment. The adsorption capacity and equilibrium binding constant were 86.92 mg g and 1.46 L mg, respectively. Based on this special structure, it can also act as a "protective umbrella" and improve the stability of C3G. Furthermore, it exhibited high selectivity compared with dummy template imprinting technique. After purification, the purity of C3G was obviously improved (from 11.96% to 84.72%). This work provided a new strategy for the selective separation of anthocyanin and a method to develop MIPs for thermosensitive substances.
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http://dx.doi.org/10.1016/j.talanta.2020.121958DOI Listing
April 2021

Carbon and Nitrogen Stable Isotopes Evidence for the Environmental Impact of the Cage Fish Farm in Poyang Lake, China.

Bull Environ Contam Toxicol 2020 Dec 17;105(6):860-867. Epub 2020 Nov 17.

School of Resources Environmental and Chemical Engineering, The Key Laboratory of Poyang Lake Environment and Resource Utilization Ministry of Education, Nanchang University, Nanchang, 330031, People's Republic of China.

The carbon and nitrogen stable isotope compositions of the sediment organic matter, fish feed and fish feces were measured to assess the impact of the aquaculture waste from the cage fish farm in Poyang Lake. The results provide evidence of the non-negligible effect of aquaculture waste on the sediment organic matter. The δC and δN of sediments varied from - 27.62 to - 25.66‰ and 4.83 to 6.92‰, respectively. The sediment organic matter had a mixed source of waste feed, fish feces and plankton. The average contribution ratio of waste feed and fish feces was 53.1% and 24.6%, respectively. The aquaculture derived organic matter ratio was high with ranging from 57.6 to 95.6%. The aquaculture waste seemed to disperse widely enough to influence the study site about 2.5 km distance from the cage. The dispersion and resuspension were the main two factors which can explain the great effect area found in this study.
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http://dx.doi.org/10.1007/s00128-020-03042-1DOI Listing
December 2020

Using High-Temporal-Resolution Ambient Data to Investigate Gas-Particle Partitioning of Ammonium over Different Seasons.

Environ Sci Technol 2020 08 31;54(16):9834-9843. Epub 2020 Jul 31.

School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332-0512, United States.

Ammonium is one of the dominant inorganic water-soluble ions in fine particulate matter (PM). In this study, source apportionment and thermodynamic equilibrium models were used to analyze the relationship between pH and the partitioning of ammonium (ε(NH)) using hourly ambient samples collected from Tianjin, China. We found a "Reversed-S curve" between pH and ε(NH) from the ambient hourly aerosol dataset when the theoretical ε(NO) (an index identified in this work) was within specific ranges. A Boltzmann function was then used to fit the Reversed-S curve. For the summer data set, when ε(NO) was between 0.7 and 0.8, the fitted was 0.88. Through thermodynamic analysis, we found that the values of [H] ( = 3.08 × 10 L mol) and ε(NO) can influence the pH-ε(NH) curve. Under certain situations, the values of [H] and ε(NO) are similar to each other, and ε(NH) is sensitive to pH, suggesting that ε(NO) plays an important role in affecting the ε(NH). During summer, winter, and spring seasons, when the relative humidity was greater than 0.36 and ε(NO) was between 0.8 and 0.95, there was an obvious Reversed-S curve, with = 0.60. The theoretical [H] and ε(NO) developed in this work can be used to analyze the gas-particle partitioning of ammonia-ammonium and nitrate-nitric acid in the ambient atmosphere. Also, it is the first time that we created the joint source-NH/HNO maps to integrate sources, aerosol pH and liquid water content, and ions (altogether in one map), which can provide useful information for designing effective strategies to control particulate matter pollution.
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http://dx.doi.org/10.1021/acs.est.9b07302DOI Listing
August 2020

Functional characterization of a low-frequency V1937I variant in FASN associated with susceptibility to esophageal squamous cell carcinoma.

Arch Toxicol 2020 06 9;94(6):2039-2046. Epub 2020 May 9.

Department of Epidemiology and Biostatistics, Key Laboratory for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Metabolic reprogramming has been regarded as one of the core hallmarks of cancer and increased de novo fatty acid synthesis has been documented in multiple tumors including esophageal squamous cell carcinoma (ESCC). Our previous exome-wide analyses found a Val1937Ile variant (rs17848945) in the 34th exon of fatty acid synthase (FASN) that showed a strong association with the risk of ESCC. In this study, we performed a series of functional assays to investigate the biological functions underlying this variant in the development of ESCC. We demonstrated that FASN was upregulated in ESCC and both knockdown and knockout of FASN significantly inhibited ESCC cell proliferation, suggesting a tumor promoter role for this gene in ESCC. Furthermore, the results showed that overexpression of FASN[I] in the ESCC cells substantially enhanced cell proliferation, compared with overexpression of FASN[V], or the control vector. Intriguingly, we found that the FASN[I] variant can enhance the enzyme activity of FASN, and, thus, increase the amount of the FASN end-product, palmitate in the ESCC cells. We also observed elevated palmitate levels in the plasma of the FASN[I] genotype carriers among a total of 632 healthy Chinese adults. In conclusion, our results suggested that the FASN V1937I variant influenced ESCC cell proliferation and susceptibility by altering the catabolic activity of FASN on palmitate. These findings may highlight an important role of palmitate metabolism in the development of ESCC and may contribute to the personalized medicine of this disease.
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http://dx.doi.org/10.1007/s00204-020-02738-xDOI Listing
June 2020

Strategy of Fusion Covalent Organic Frameworks and Molecularly Imprinted Polymers: A Surprising Effect in Recognition and Loading of Cyanidin-3--glucoside.

ACS Appl Mater Interfaces 2020 Feb 7;12(7):8751-8760. Epub 2020 Feb 7.

School of Chemistry and Chemical Engineering , Harbin Institute of Technology , 92 West Dazhi Street , Nangang District, Harbin , Heilongjiang 150001 , People's Republic of China.

Seven magnetic covalent organic framework molecularly imprinted polymers with magnetic responsiveness, large specific surface area, and molecular recognition ability were successfully synthesized, suitable for the separation of cyanidin-3--glucoside from complex samples. Using a room-temperature synthesis strategy, covalent organic frameworks accompanied by imprinted sites were formed in one step on the functionalized magnetic nanoparticles with 1,6-hexanediamine. The composite materials were easy to prepare and showed high adsorption capacity (127 mg g) together with favorable adsorption selectivity for cyanidin-3--glucoside over other flavonoids and phenolic acids. Magnetic covalent organic framework molecularly imprinted polymers exhibited good stability in 10 adsorption-desorption cycles. This work established a new method of extracting and separating high-purity anthocyanins and even unstable natural compounds.
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http://dx.doi.org/10.1021/acsami.9b21460DOI Listing
February 2020

Simultaneous determination of alflutinib and its active metabolite in human plasma using liquid chromatography-tandem mass spectrometry.

J Pharm Biomed Anal 2019 Nov 16;176:112735. Epub 2019 Jul 16.

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201210, China; University of Chinese Academy of Science, Beijing 100049, China. Electronic address:

Alflutinib, or known as AST2818, is an irreversible tyrosine kinase inhibitor that selectively inhibits EGFR mutations, especially T790M. At present, alflutinib has undergone phase II/III clinical trials for non-small cell lung cancer (NSCLC) treatment in China. The present study aimed to analye the pharmacokinetics of alflutinib and its active metabolite AST5902 in a plasma sample of NSCLC patient. A sensitive and highly selective method was optimized and validated for the detection of alflutinib and AST5902 using a liquid chromatography-tandem mass spectrometry. After precipitating proteins with acetonitrile, alflutinib, AST5902 and AST2818-d (internal standard) were analyzed with a Waters BEH C column. The mobile phase was optimized with acetonitrile: ammonium acetate (2 mmol/L) containing 0.2% formic acid using gradient elution. Separation was achieved within a total chromatographic running time of 2.1 min. Quantification was carried out using positive ion multiple reaction monitoring mode at ion transitions m/z 569.3→441.2, 555.1→498.2 and 572.3→441.2 for alflutinib, AST5902 and AST2818-d, respectively. An excellent linearity was observed for alflutinib and AST5902 within concentration ranges of 0.20-100 and 0.050-25.0 ng·mL, respectively. Notably, the lower limit of quantification for alflutinib and AST5902 were 0.20 and 0.050 ng/mL, respectively. The intra- and inter-day accuracy of alflutinib were 0.7-2.9%, while its intra- and inter-assay precision were ≤9.1% and ≤10.5%, respectively. The accuracy of AST5902 was within -0.2-3.9%, while the intra- and inter-assay precision were ≤8.0% and ≤8.6%, respectively. The recoveries of the analysts remained constant and could be reproduced at different concentrations. Furthermore, this analytical method could be applied to determine the pharmacokinetic analysis of alflutinib and AST5902 in human plasma.
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http://dx.doi.org/10.1016/j.jpba.2019.06.032DOI Listing
November 2019

[Synthesis and applications of epoxy-functionalized bi-functional magnetic molecularly imprinted polymers for polysaccharide adsorption].

Se Pu 2019 Jul;37(7):673-682

School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150090, China.

A polysaccharide-imprinted nanoparticle composite material with bi-functional monomers molecularly imprinted polymers (Bi-MMIPs) was successfully synthesized in aqueous solution by using starch as the template, 3-aminobenzeneboronic acid (APBA) as the functional monomer, 2-acrylamide-2-methylpropanesulfonic acid (AMPS) as the co-functional monomer, and ammonium persulfate (APS) as the initiator. Transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Fourier-transform infrared (FT-IR) spectroscopy were used to characterized Bi-MMIPs. The adsorption and recognition characteristics of Bi-MMIPs for starch were investigated in detail by using the static method, dynamic method, and competitive adsorption experiments. The TEM, SEM and FT-IR results showed that the two functional monomers were loaded on the surface of Bi-MMIPs. Bi-MMIPs possessed very strong adsorption affinity and specific recognition for starch. The saturated adsorption capacity reached 13.88 mg/g, and the selectivity coefficients relative to glucosans 5000 Da and 70000 Da were 2.67 and 3.77, respectively. Despite the imprinting factor of Bi-MMIPs (α=3.04), Bi-MMIPs were regenerated easily and exhibited excellent recognition, selectivity, and reusability for adsorbing starch. To elucidate the mechanism, APBA and AMPS showed synergy effect in synthesizing bi-functional monomers by providing reversible covalent bonds and hydrogen bonds, respectively, which could effectively enhance the spatial arrangement of combining sites for template molecular.
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http://dx.doi.org/10.3724/SP.J.1123.2019.01018DOI Listing
July 2019

Inhibits -Induced Corrosion via Biomineralization in Seawater.

Front Microbiol 2019 21;10:1111. Epub 2019 May 21.

College of Ocean Science and Engineering, Shanghai Maritime University, Shanghai, China.

The marine bacterium, , grows quickly in a marine environment and can significantly accelerate the corrosion of steel materials. Here, we present an approach to inhibit -induced corrosion by biomineralization. The corrosion of steel is mitigated in seawater via the formation of a biomineralized film induced by . The film is composed of extracellular polymeric substances (EPS) and calcite, exhibiting stable anti-corrosion activity. The microbial diversity and medium chemistry tests demonstrated that the inhibition of growth by was essential for the formation of the biomineralized film.
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http://dx.doi.org/10.3389/fmicb.2019.01111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536734PMC
May 2019

Rapid Discovery of the Potential Toxic Compounds in by UHPLC/Q-Orbitrap-MS-Based Metabolomics and Correlation Analysis.

Front Pharmacol 2019 16;10:329. Epub 2019 Apr 16.

CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan, China.

The dry roots of (PM), involving both the raw and processed materials, are widely used as the traditional Chinese medicine for treating various diseases in China. Hepatotoxicity has been occasionally reported in patients who consume PM. Unfortunately, no definite criteria are currently available regarding the processing technology of PM for reduction the toxicity. In this work, we aimed to investigate the variations of PM metabolite profiles induced by different processing technologies by UHPLC/Q-Orbitrap-MS and multivariate statistical analysis, and to discover the potential toxic compounds by correlating the cytotoxicity of L02 cell with the contents of metabolites in raw and processed PM samples. We could identify two potential toxic compounds, emodin-8--glucoside and torachrysone--hexose, which could be selected as the toxic markers to evaluate different processing methods. The results indicated all processed PM samples could decrease the cytotoxicity on L02 cell. The best processing technology for PM process was to steam PM in black soybean decoction (BD-PM) for 24 h.
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http://dx.doi.org/10.3389/fphar.2019.00329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6477936PMC
April 2019

High-Resolution Data Sets Unravel the Effects of Sources and Meteorological Conditions on Nitrate and Its Gas-Particle Partitioning.

Environ Sci Technol 2019 03 7;53(6):3048-3057. Epub 2019 Mar 7.

School of Civil and Environmental Engineering , Georgia Institute of Technology , Atlanta , Georgia 30332-0512 , United States.

Nitrate is one of the most abundant inorganic water-soluble ions in fine particulate matter (PM). However, the formation mechanism of nitrate in the ambient atmosphere, especially the impacts of its semivolatility and the various existing forms of nitrogen, remain under-investigated. In this study, hourly ambient observations of speciated PM components (NO, SO, etc.) were collected in Tianjin, China. Source contributions were analyzed by PMF/ME2 (Positive Matrix Factorization using the Multilinear Engine 2) program, and pH were estimated by ISORROPIA-II, to investigate the relationship between pH and nitrate. Five sources (factors) were resolved: secondary sulfate (SS), secondary nitrate (SN), dust, vehicle and coal combustion. SN and pH showed a triangle-shaped relationship. When SS was high, the fraction of nitrate partitioning into the aerosol phase exhibits a characteristic "S-curve" relationship with pH for different seasons. An index ( I) is developed and combined with pH to explore the sensitive regions of "S-curve". Controlling the emissions of anions (SO, Cl), cations (Ca, Mg, etc.) and gases (NO , NH, SO, etc.) will change pH, potentially reducing or increasing SN. The findings of this work provide an effective approach for exploring the formation mechanisms of nitrate under different influencing factors (sources, pH, and I).
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http://dx.doi.org/10.1021/acs.est.8b06524DOI Listing
March 2019

Amido surface-functionalized magnetic molecularly imprinted polymers for the efficient extraction of Sibiskoside from Sibiraea angustata.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Mar 18;1109:90-98. Epub 2019 Jan 18.

School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150090, China. Electronic address:

A general method for efficient and selective extraction of a target compound from complex natural products remains elusive, despite decades of research. By introducing a functional amido group on the surface of dispersity-enhanced magnetic nanoparticles, a nanoparticle receptor to selectively recognize Sibiskoside (a monoterpene) from the aerial portion of Sibiraea angustata by hydrogen bond interaction was synthesized. The superparamagnetic FeO nanoparticles were successively modified with tetraethyl orthosilicate (TEOS), amino and amido functional groups, and 4-vinylbenzoic acid (VBZA) was used as the functional monomer. A thin layer of poly (VBZA) imprinted with Sibiskoside was immobilized on the surface of magnetic carriers. Attributing to the amido group introduced into the magnetic particles, the template could attract and bind to the surface and promote the formation of a hydrogen bond system between the carrier, template molecules and functional monomer. High-density molecular recognition sites grew on the surface of magnetic substrate. The adsorption reached equilibrium at approximately 150 min, while fast adsorption occurred during the first 60 min. The maximum adsorption capacity has been found to be 13.75 mg g according to calculation with the Langmuir isotherm. The selectivity coefficients of Molecular imprinting polypers (MIPs) for Sibiskoside with respect to Andrographolide, Loganin, Gastrodin, geraniol-1-O-[α-l-rhamnopyranosyl-(1 → 6)-1-β-d-glucopyranoside] (GRG), Sibiscolacton and Sibiraic acid were 2.26, 1.43, 1.701.56, 1.05, 0.73 and, respectively. The results indicated that the MIPs possessed good specific adsorption capacity and selectivity toward Sibiskoside and had the potential to be a candidate for the separation and purification of monoterpenes from Sibiraea angustata, which is of great significance to obesity management.
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http://dx.doi.org/10.1016/j.jchromb.2019.01.009DOI Listing
March 2019

Calcium‑sensing receptors in human peripheral T lymphocytes and AMI: Cause and effect.

Int J Mol Med 2018 Dec 10;42(6):3437-3446. Epub 2018 Oct 10.

Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150086, P.R. China.

Acute myocardial infarction (AMI) is a disease associated with inflammation. T lymphocytes are involved by secreting cytokines and inflammatory factors. In our previous study, it was found that the T lymphocytes exhibited certain functional changes, the onset of which was induced by modulating calcium‑sensing receptor (CaSR) in AMI. In the present study, western blotting was used to verified the expression of T lymphocyte CaSR and pathway proteins, including phosphorylated extracellular signal‑regulated kinase (P‑ERK)1/2 and phosphorylated c‑Jun N‑terminal kinase (P‑JNK), and used cytometric bead array to detect the secretion of interleukin (IL)‑4, IL‑6, IL‑10 and tumor necrosis factor (TNF)‑α in AMI onset, the results demonstrated that they were all increased. In addition, the expression of T lymphocyte pathway proteins, including P‑ERK1/2 and P‑JNK, and the secretion of IL‑4, IL‑6, IL‑10 and TNF‑α decreased after T lymphocytes being transfected by CaSR small interfering RNA. By contrast, the neonatal mouse cardiomyocytes under hypoxia and hypoxia/re‑oxygenation exhibited ultrastructural damage, increased apoptosis, increased production of lactate dehydrogenase (LDH) and malondialdehyde, and reduced superoxide dismutase; these indicators changed extensively when cardiomyocytes were co‑cultured with T lymphocytes. However, the effects were reversed when the cardiomyocytes were co‑cultured with CaSR‑silenced T lymphocytes. These results indicated that CaSR may modulate T lymphocytes to release cytokines through mitogen‑activated protein kinase pathways and affect cardiomyocyte injury. The relationship between AMI and T lymphocyte CaSR is reciprocal.
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http://dx.doi.org/10.3892/ijmm.2018.3924DOI Listing
December 2018

Aerosols in an arid environment: The role of aerosol water content, particulate acidity, precursors, and relative humidity on secondary inorganic aerosols.

Sci Total Environ 2019 Jan 24;646:564-572. Epub 2018 Jul 24.

School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

Meteorological conditions, gas-phase precursors, and aerosol acidity (pH) can influence the formation of secondary inorganic aerosols (SIA) in fine particulate matter (PM). Most works related to the influence of pH and gas-phase precursors on SIA have been laboratory research, but field observation research is very scarce, especially in arid environments. The relationship among SIA, pH, gas-phase precursors, and meteorological conditions are investigated in Hohhot, a major city in China with an arid environment. Secondary inorganic species, e.g., SO, NO, were typically found at low levels, reflecting the low level of secondary aerosol. It is interesting to note that the level of SO in Hohhot was higher than in other cities while SO was relatively lower than in other cities. Multiple receptor models were used to explore the contributions to the SIA and quantify the source impacts on the SIA. Annual average aerosol pH in Hohhot was 5.6 (range 1.1-8.4) which was estimated by a thermodynamic equilibrium model. Additionally, a statistical method was used to evaluate the influence of SIA sources on ambient aerosol concentrations. Aerosol water content and particulate acidity were found to be positively associated with secondary SO, while NO and RH had a significant impact on secondary NO in an arid atmosphere. The findings explain the relationship between gaseous precursors, relative humidity, aerosol pH and temperature in the arid city of Hohhot.
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http://dx.doi.org/10.1016/j.scitotenv.2018.07.321DOI Listing
January 2019

Dietary Fiber Intake and Endometrial Cancer Risk: A Systematic Review and Meta-Analysis.

Nutrients 2018 Jul 22;10(7). Epub 2018 Jul 22.

CAS Key Laboratory of Nutrition, Metabolism and Food safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yueyang Road, New Life Science Building, Room A1926, Shanghai 200031, China.

Epidemiological studies are inconclusive regarding the association between dietary fiber intake and endometrial cancer risk. Thus, we aimed to conduct a meta-analysis to clarify the association between dietary fiber and endometrial cancer risk. We searched the PubMed and ISI Web databases for relevant studies through March 2018. The association between dietary fiber and endometrial cancer risk was evaluated by conducting a meta-analysis including 3 cohort and 12 case⁻control studies. A significant negative association was observed between total dietary fiber intake and endometrial cancer risk in 11 case⁻control studies (odds ratios (OR) 0.76, 95% confidence interval (CI): 0.64⁻0.89, ² = 35.2%, = 0.117), but a marginal positive association was observed in three cohort studies (relative risk (RR) 1.22, 95% CI: 1.00⁻1.49, ² = 0.0%, = 0.995). Particularly, a negative association was observed in North America (OR = 0.70, 95% CI: 0.59⁻0.83, ² = 8.9%, = 0.362). In addition, a positive association was observed in cereal fiber (RR = 1.26, 95% CI: 1.03⁻1.52, ² = 0.0%, = 0.530, 3 cohort studies) and a negative association was observed in vegetable fiber (OR = 0.74, 95% CI: 0.58⁻0.94, ² = 0.0%, = 0.445, 3 case⁻control studies). In conclusion, negative associations with endometrial cancer risk were observed for higher total dietary fiber intake and higher vegetable fiber intake in the case⁻control studies. However, results from the cohort studies suggested positive relationships of higher total fiber intake and higher cereal fiber intake with endometrial cancer risk.
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http://dx.doi.org/10.3390/nu10070945DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6073518PMC
July 2018

Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils.

Oxid Med Cell Longev 2017 10;2017:3869561. Epub 2017 Sep 10.

Department of Clinical Laboratory, Harbin Medical University Cancer Hospital, Harbin 150086, China.

The calcium-sensing receptors (CaSRs) play an important role in many tissues and organs that are involved in inflammatory reactions. Peripheral blood polymorphonuclear neutrophils (PMNs) are important inflammatory cells. However, the expression and functions of CaSR in peripheral blood PMNs are still not reported. In this study, we collected rat peripheral blood PMNs to observe the relationship between CaSR and PMNs. From the results, we found first that the CaSR protein was expressed in PMNs, and it increased after PMNs were activated with fMLP. In addition, CaSR activator cincalcet promoted the expression of CaSR and P-p65 (NF-B signaling pathway protein) and Bcl-xl (antiapoptosis protein), and it increased the secretion of interleukin-6 (IL-6) and myeloperoxidase (MPO); meanwhile, it decreased proapoptosis protein Bax expression and the production of IL-10 and reactive oxygen species (ROS). At the same time, cincalcet also decreased the PMN apoptosis rate analyzed by flow cytometry. However, CaSR inhibitor NPS-2143 and NF-B signaling pathway inhibitor PDTC reverse the results cited earlier. All of these results indicated that CaSR can regulate PMN functions and status to play a role in inflammation, which is probably through the NF-B signaling pathway.
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http://dx.doi.org/10.1155/2017/3869561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5610836PMC
July 2018

The oxidation metabolites of endomorphin 1 and its fragments induced by free radicals.

J Pept Sci 2009 May;15(5):337-44

School of Life Science and State Key Laboratory of Applied Organic Chemistry, School of Basic Medical Sciences, Institute of Biochemistry and Molecular Biology, Lanzhou University, Lanzhou 730000, China.

Endomorphin 1 (EM1), an endogenous micro-opioid receptor agonist, acts as a free radical scavenger in vitro and an antioxidant in vivo. The modification of EM1 by ROS and the properties of the OM attracted our attention. In vitro assays were performed via RP-HPLC, spectrophotometric measurements, EPR and amino acid analysis, Schmorl's reaction to define the formation of melanin-like compounds transformed from EM1, collectively named EM1-melanin and by solubility assay, radioligand-binding assay, NADH oxidation, superoxide anion scavenging assay to study some physical and chemical properties of EM1-melanin. Possible pathways of the formation of EM1-melanin were proposed.
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http://dx.doi.org/10.1002/psc.1116DOI Listing
May 2009

Endomorphin 1 effectively protects cadmium chloride-induced hepatic damage in mice.

Toxicology 2008 Sep 25;251(1-3):35-44. Epub 2008 Jul 25.

Key Laboratory of Preclinied Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Institute of Biochemistry and Molecular Biology and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China.

The antioxidative capacity of endomorphin 1 (EM1), an endogenous mu-opioid receptor agonist, has been demonstrated by in vivo assays. The present study reports the effect of EM1 on hepatic damage induced by cadmium chloride (Cd(II)) in adult male mouse. Mouse were given intraperitoneally (i.p.) a single dose of Cd(II) (1mg/kg body weight per day) and the animals were co-administrated with a dose of EM1 (50 microM/kg body weight per day) for 6 days. Since hepatic damage induced by Cd(II) is related to oxidative stress, lipid peroxidation (LPO), protein carbonyl (PCO), superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) were evaluated. The parameter indicating tissue damage such as liver histopathology was also determined. In addition, the concentrations of Cd and zinc (Zn) in the liver were analyzed. The intoxication of Cd(II) lead to the enhanced production of LPO and PCO, treatment with EM1 can effectively ameliorate the increase of LPO and PCO compared to the Cd(II) group. The increased activities of CAT, SOD and the elevated GSH induced by Cd(II) may relate to an adaptive-response to the oxidative damage, the effect of EM1 can restore the elevated antioxidant defense. Our results suggested that the structure features and the ability of chelating metal of EM1 may play a major role in the antioxidant effect of EM1 in vivo and opioid receptors may be involved in the protection of hepatic damage induced by Cd(II).
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http://dx.doi.org/10.1016/j.tox.2008.07.051DOI Listing
September 2008

Endomorphins and morphine limit anoxia-reoxygenation-induced brain mitochondrial dysfunction in the mouse.

Life Sci 2008 Mar 24;82(13-14):752-63. Epub 2008 Jan 24.

Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Institute of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Lanzhou University, 222 Tianshui South Road, Lanzhou, 730000, PR China.

The protection of brain mitochondria from oxidative stress is an important therapeutic strategy against ischemia-reperfusion injury and neurodegenerative disorders. Isolated brain mitochondria subjected to a 5 min period of anoxia followed by 5 min reoxygenation mirrored the effect of oxidative stress in the brain. The present study attempts to evaluate the protective effects of endomorphin 1 (EM1), endomorphin 2 (EM2), and morphine (Mor) in an in vitro mouse brain mitochondria anoxia-reoxygenation model. Endomorphins (EM1/2) and Mor were added to mitochondria prior to anoxia or reoxygenation. EM1/2 and Mor markedly improved mitochondrial respiratory activity with a decrease in state 4 and increases in state 3, respiratory control ratio (RCR) and the oxidative phosphorylation efficiency (ADP/O ratio), suggesting that they may play a protective role in mitochondria. These drugs inhibited alterations in mitochondrial membrane fluidity, lipoperoxidation, and cardiolipin (CL) release, which indicates protection of the mitochondrial membranes from oxidative damage. The protective effects of these drugs were concentration-dependent. Furthermore, these drugs blocked the enhanced release of cytochrome c (Cyt c), and consequently inhibited the cell apoptosis induced by the release of Cyt c. Our results suggest that EM1/2 and Mor effectively protect brain mitochondria against oxidative stresses induced by in vitro anoxia-reoxygenation and may play an important role in the prevention of deleterious effects during brain ischemia-reperfusion and neurodegenerative diseases.
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http://dx.doi.org/10.1016/j.lfs.2008.01.004DOI Listing
March 2008
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