Publications by authors named "Qianwen Chen"

10 Publications

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Genetic association analysis between IL9 and coronary artery disease in a Chinese Han population.

Cytokine 2021 Nov 20;150:155761. Epub 2021 Nov 20.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

Interleukin-9 (IL-9) plays important role in coronary artery disease (CAD). However, the exact relationship between them is not explored yet. Here, four tag SNPs covering IL9 (rs31563, rs2069868, rs2069870 and rs31564) were selected to conduct case-control association analyses in a total of 3704 individuals from Chinese Han population (1863 CAD vs 1841 control). Results showed that: first, rs2069868 was associated with CAD combined with hypertension (P = 0.027); second, IL9 haplotype (CGAT) was associated with CAD (P = 0.035), and the combination genotype of "rs31563_CC/rs31564_TT" would remarkably decrease the risk of CAD (P = 0.001); third, significant associations were found between rs2069870 and decreased LDL-c levels and decreased total cholesterol levels, and between rs31563 and increased HDL-c levels (P < 0.05). Therefore, we conclude that IL9 might play a causal role in CAD by interacted with CAD traditional risk factors, which might confer a new way to improve the prevention and treatment of CAD.
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http://dx.doi.org/10.1016/j.cyto.2021.155761DOI Listing
November 2021

Observation of the curative effect of Guizhi-Shaoyao-Zhimu decoction combined with methotrexate in the treatment of early rheumatoid arthritis based on ultrasonic evaluation: study protocol of a randomized, double-blind, controlled clinical trial.

Trials 2021 Nov 3;22(1):764. Epub 2021 Nov 3.

Department of Rheumatism, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200021, China.

Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease with the primary clinical symptoms of joint swelling and pain. Early detection of erosion and synovial inflammation at an active stage resulting from RA can prevent damage to the joints and activity restriction. However, there are still many patients who do not respond to these treatments; the development of newer, safer drugs is urgently needed. Compared to Western medicine, Guizhi-Shaoyao-Zhimu decoction has equal or higher efficacy and safety for RA patients. With the widespread use of musculoskeletal ultrasound (MSUS), this technology holds great value for the degree of joint damage in RA patients, guiding the clinical selection of treatments, and assessing of prognosis. Therefore, we designed a double-blinded randomized controlled clinical trial to measure the safety and efficacy of Guizhi-Shaoyao-Zhimu decoction in treating early-stage RA using MSUS.

Methods: This study is a randomized, double-blinded, parallel group, placebo-controlled trial. A total of 152 adult participants with early RA will be enrolled, with balanced treatment allocation (1:1). The experimental intervention will be Guizhi-Shaoyao-Zhimu decoction plus the conventional medicine methotrexate and the control intervention will be placebo plus the conventional drug methotrexate for 3 months. In addition, both groups will receive folic acid during treatment to prevent side effects from methotrexate. The primary outcomes are the erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), hepatic and renal function, visual analog scale, disease activity score in 28 joints, measurement scale for TCM symptoms, and 7-joint ultrasound score.

Discussion: We designed this double-blinded randomized controlled clinical trial to evaluate the efficacy and safety of Guizhi-Shaoyao-Zhimu decoction in RA patients using MSUS. The results of this trial may provide insights into how to improve the clinical symptoms of RA patients and delay further joint destruction. We hope that this trial may provide preliminary evidence of the efficacy of Guizhi-Shaoyao-Zhimu decoction in treating RA patients and that these results aid researchers, practitioners, and patients alike.

Aim: The main aim of the study is to clarify the efficacy and safety of Guizhi-Shaoyao-Zhimu decoction in patients with early RA.

Trial Registration: Chinese Clinical Trials Register ChiCTR2000036141 . Registered on 21 August 2020 (retroactively registered).
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http://dx.doi.org/10.1186/s13063-021-05579-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565030PMC
November 2021

Short- and medium-chain chlorinated paraffins in soil from an urban area of northern China: Levels, distribution, and homolog patterns.

Sci Total Environ 2021 Oct 8;807(Pt 2):150833. Epub 2021 Oct 8.

Jianghan University, Wuhan 430056, China. Electronic address:

Short-chain chlorinated paraffins (SCCPs) are persistent organic pollutants that are present in relatively high concentrations in various environmental media in China. Many studies have focused on chlorinated paraffins in soil from agricultural land and contaminated areas. There are limited data on the levels of chlorinated paraffins in soil from urban areas. In this study, to investigate the levels, distribution, and homolog patterns of chlorinated paraffins (CPs) in soil from a typical urban area, 130 soil samples were collected and combined to form 26 pooled samples. The samples were analyzed for 50 CP congener groups (CCl). The concentration ranges for SCCPs, medium-chain CPs (MCCP), and chlorinated nonane paraffin (C-CP) were 19-1456 ng/g (average: 234 ng/g), <10-385 ng/g (average: 54 ng/g), and 1-39 ng/g (average: 11 ng/g), respectively. The CP concentrations were not significantly correlated with the total organic carbon content (P > 0.05). Compared with other areas worldwide, the SCCP and C-CP concentrations in soil in this area were at the medium level, and the concentrations of MCCPs were at a low level. The CP concentrations were higher in soil samples collected near factories and domestic garbage disposal sites. CCl were the main SCCP homologs and CCl were the main MCCP homologs. Principal component analysis showed that the sources of C-CPs, SCCPs, and MCCPs in the soils were similar. Risk assessment showed that the concentrations of SCCPs and MCCPs in soil in this area did not pose a significant risk to soil organisms or human health.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150833DOI Listing
October 2021

First Report of Leaf Rust of Caused by in China.

Plant Dis 2021 Feb 22. Epub 2021 Feb 22.

Northwest Agriculture and Forestry University, 12469, College of Landscape Architecture and Art, Yangling, Shaanxi, China;

Maxim, mainly distributed in China, Japan, Korea and eastern Russia (Shang et al. 2012), is a widely planted ornamental and pharmaceutical tree (Zhang et al. 2015). In September 2020, leaf samples of infected by uredinia were collected in Shaanxi Province (34°15'40.06'' N, 108°3'54.54'' E, alt. 432.35m), China. Telia development was observed in late autumn. Voucher specimens were deposited in the Herbarium Mycologicum Academiae Sinicae (no. HMAS249354), China. This led to premature defoliation and in the 90% planting wide incidence. Geospatial investigations revealed that this rust was widely distributed in local urban parks, but was nonpathogenic to , , , and . This fungus was morphologically characterized and most closely matched descriptions of . Uredinia were hypophyllous, subepidermal, scattered to gregarious, oval or round, 0.10-0.30 × 0.08-0.15 mm, golden yellow to orange, somewhat pulverulent. Peridia were hemispherical, erumpent with apical pores; peridial cells minute, irregularly polygonal, hyaline to pale yellow; ostiolar cells ellipsoid or roundish. Urediniospores were subglobose, ovate or ellipsoid, 20-33 × 15-21 μm, yellow to pale orange; wall 1-2 μm thick, hyaline to pale yellow, echinulate, somewhere smooth. Pedicels were deciduous, hyaline, minute, fragile. Telia were hypophyllous, subepidermal, intermixed with uredinia, irregularly polygonal, restricted by veins, 0.34-0.91 × 0.21-0.54 mm, and orange to amber brown. Teliospores were produced parallelly single-layered, and were subglobose, oblong, sometimes angular, 23-47 × 16-34 μm, colorless to pale yellow, 1-5 mediastinal, 2-6-celled; lateral wall 1-1.6 μm thick, apical wall 1-3 μm thick, smooth, hyaline. The internal transcribed spacer (ITS) and rDNA-28S regions were amplified using ITS1F/ITS4 and NL1/NL4 (Ji et al. 2019) to confirm the identification. The aligned sequences were deposited in GenBank (accession no. MW391829, MW543709, MW541916, MW541917). Phylogenetic trees were constructed based on neighbor-joining (NJ), maximum-likelihood (ML) and Bayesian methods. ML and NJ bootstrap values were calculated by bootstrap analyses of 1,000 replicates with GTR+G+I model using MEGA-X (Kumar et al. 2018), while Bayesian Markov chain Monte Carlo analyses were performed using MrBayes ver. 3.1.2 (Huelsenbeck & Ronquist 2001; Ji et al. 2019). Phylogenetic analysis revealed that HMAS249354 and were grouped into one clade highly supported by bootstrap values of NJ, ML, and Bayesian posterior probability (Bpp) of 97%/93%/1, respectively. Koch's postulates were fulfilled with 1-year-old healthy plants of . Fresh urediniospores were collected and suspended in a 0.05% water solution of Tween 20, and 100 μl of urediniospores suspension (10 urediniospores/ml) per leaf (n=10) were sprayed, with another ten healthy leaves sprayed with sterile water as the control. The plants were placed in dark for 48 h and then moved into greenhouse at 22°C with 12 h light per day. Disease symptoms after 10-12 days' inoculation on the inoculated leaves which were identical to the original observations, while the control leaves remained healthy. Previously, was reported to infect Sieb. et Zucc. in Japan (Hiratsuka 1940) and Hayata in Taiwan, China (Dai 1979). This is the first report of leaf rust of caused by Hirats. f. in China.
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http://dx.doi.org/10.1094/PDIS-12-20-2708-PDNDOI Listing
February 2021

Systematic profiling of the effective ingredients and mechanism of Scabiosa comosa and S. tschilliensis against hepatic fibrosis combined with network pharmacology.

Sci Rep 2021 01 28;11(1):2600. Epub 2021 Jan 28.

Department of Pharmacy, Medical University of Inner Mongolia, Jinshan Development Zone, Hohhot, Inner Mongolia, China.

Scabiosa comosa and S. tschilliensis (SCST) are traditionally used for liver diseases in Mongolian medicine. However, their active ingredients and molecular mechanisms are unknown. The present study employed network pharmacology and experimental verification approaches to decipher the common pharmacological mechanisms of SCST on liver fibrosis, which is the key step in liver diseases. We predicted the targets of all available SCST ingredients with the SWISS and SuperPred servers and clustered the targets related to liver fibrosis from DrugBank, the OMIM database and the literature. We further evaluated the links between the herbal ingredients and pharmacological actions to explore the potential mechanism of action of SCST. We found that the PPARG signalling pathway could be regulated by SCST for liver fibrosis through enrichment analysis. The key targets included 8 co-targets, including HSP90AA1, PPARG, HSP90AB1, STAT1, etc., which play pivotal roles in the pathogenesis of liver fibrosis. Additionally, the top 15 key compounds included flavonoids and phenylpropanoids. Central to the pathogenesis of liver fibrosis is trans-differentiation or activation of hepatic stellate cells (HSCs). Therefore, LX2 cells, an immortalized human HSC line, were studied. Here, a total 37 components were isolated and identified from the inflorescences of SCST, including the new compound tschilliensisin, and the first separated components, β-sitosterol and luteolin, and these compounds were assessed against anti-hepatic fibrosis. An MTT assay and quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting analyses demonstrated that the flavonoids of SCST revealed anti-hepatic fibrosis effects via anti-proliferation and increases in the Stat1, Pparg, Hsp90aa1 genes and STAT1 and PPARG proteins in LX-2 cells. In conclusion, these results indicate that SCST has multi-targeted and multi-component synergistic anti-hepatic fibrosis effects.
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http://dx.doi.org/10.1038/s41598-021-81399-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843997PMC
January 2021

Sulfate modified g-CN with enhanced photocatalytic activity towards hydrogen evolution: the role of sulfate in photocatalysis.

Phys Chem Chem Phys 2020 May 28;22(18):10116-10122. Epub 2020 Apr 28.

School of Materials, State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-Sen University, Guangzhou 510275, China.

Sulfate modified graphitic carbon nitride (g-CN) was prepared by simple co-pyrolysis of dicyandiamide and ammonium sulfate, and shows seven times higher photocatalytic activity towards hydrogen production than pristine g-CN. The origin of its improved photocatalytic activity was comprehensively investigated, and it was found that there are two kinds of sulfate (strongly adsorbed sulfate and a weakly adsorbed one) in the modified sample, both of which play important but slightly different roles in the photocatalysis. Compared to the strongly adsorbed one, the weakly adsorbed sulfate is more beneficial for charge separation and thus promotes more electrons to participate in the photocatalytic reaction. By applying the above synthesis method, most sulfate in our best photocatalyst exists as weakly adsorbed species, which is confirmed by advanced characterization techniques as well as DFT calculations. The increased number of electrons and improved charge separation, which are induced by the weakly adsorbed sulfate, are key to boosting the photocatalytic activity of g-CN. Hence, this work provides comprehensive insights into the effect of sulfate on the photocatalytic activity of g-CN, which help in the design of more efficient photocatalysts by suitable surface modification.
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http://dx.doi.org/10.1039/c9cp07002hDOI Listing
May 2020

COL2A1 protective variant reduces sporadic rhegmatogenous retinal detachment severity.

Exp Eye Res 2020 02 31;191:107907. Epub 2019 Dec 31.

Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China. Electronic address:

Rhegmatogenous retinal detachment (RRD) is the most common type of RD, the separation of neurosensory retina from the underlying retinal pigment epithelium. The RRD patients can be benefited from appropriate treatment if detected early, especially for the people predicted at high risk. In this study, we aimed to investigate the genetic association and clinical correlation of collagen type II alpha 1 (COL2A1) variants with sporadic RRD in a southern Chinese population. Totally 156 RRD patients and 254 control subjects were recruited, and 12 COL2A1 tag single nucleotide polymorphisms were genotyped by the TaqMan assay. The RRD patients had poorer visual acuity (P < 0.001) and lower intraocular pressure (IOP; P < 0.001) in their surgical eyes compared to the fellow eyes. The COL2A1 rs1793958 variant was significantly associated with RRD in the genotypic (P = 0.024), allelic (P = 0.011, odds ratio (OR) = 0.669), recessive (P = 0.011, OR = 0.384) and homozygous models (P = 0.007, OR = 0.348). RRD patients carrying the rs1793958 G allele had smaller retinal detachment area (P = 0.041) and smaller IOP differences (P = 0.046) between the surgical and fellow eyes compared to those carrying the wildtype AA genotype. In summary, this study revealed that the COL2A1 rs1793958 variant is associated with reduced risk of sporadic RRD, and patients carrying rs1793958 G allele have lower RRD severity.
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http://dx.doi.org/10.1016/j.exer.2019.107907DOI Listing
February 2020

Hybrid Protective Layer for Stable Sodium Metal Anodes at High Utilization.

ACS Appl Mater Interfaces 2019 Oct 2;11(41):37693-37700. Epub 2019 Oct 2.

Shenzhen Key Laboratory of Organic Pollution Prevention and Control, Environmental Science and Engineering Research Center , Harbin Institute of Technology , Shenzhen , Guangdong 518055 , China.

Na metal is a promising anode for Na batteries owing to its high theoretical capacity and low reduction potential. Nevertheless, an unstable and inhomogeneous solid electrolyte interphase originated from the instantaneous reactions between the Na metal anode and organic liquid electrolyte causes the intractable hurdles of dendrite growth and low Coulombic efficiency. Here, a sodium fluoride (NaF)-poly(vinylidene difluoride) (PVDF) inorganic-organic hybrid protective layer is constructed on a commercial Cu current collector via a simple blade-coating technique. A flexible PVDF matrix can endure volume change, maintaining the integrity of the anode/coating interface, while NaF particles provide improved Na diffusion conductivity and mechanical strength, suppressing the dendrite initiation and growth. Based on these synergetic effects, an excellent cycle life of more than ∼2100 h is realized at 1 mA cm at 50% depth of discharge (DOD), which outperforms 10-fold lifetime of the Cu current collector (∼170 h). Moreover, the Cu current collector with a NaF-PVDF protective layer also delivers good cycling stability at 5 mA cm and an ultrahigh DOD (80%). The rational design of the hybrid protective layer offers a new approach to realize stable Na metal batteries.
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http://dx.doi.org/10.1021/acsami.9b12059DOI Listing
October 2019

Design, synthesis and biological evaluation of novel neuchromenin analogues as potential antifungal agents.

Eur J Med Chem 2019 Jul 14;173:228-239. Epub 2019 Apr 14.

Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry and Pharmacy, Northwest A&F University, Yangling, 712100, Shaanxi Province, China. Electronic address:

In continuation of our program to discover new potential antifungal agents, thirty-two neuchromenin analogues were synthesized and characterized by the spectroscopic analysis. By using the mycelium growth rate method, the target compounds were evaluated systematically for antifungal activities in vitro against six plant pathogenic fungi, and structure-activity relationships (SAR) were derived. Compounds 6b-c, and 6l showed obvious inhibition activity on each of the fungi at 50 μg/mL. For the corresponding fungi, 7 of the compounds showed average inhibition rates of >80% at 50 μg/mL; especially, compounds 6b, 6d-e, and 6i-l displayed more potent antifungal activity against A. solani than that of thiabendazole (a positive control). Moreover, compound 6c also exhibited good activity against C. lunata with EC values of 12.7 μg/mL, and the value was much superior to that of thiabendazole (EC = 59.7 μg/mL). SAR analysis showed that the presence of conjugated structure, bearing a C=C bond conjugated to the C=O group, obviously decreased the activity; the type and position of the substituted R significantly influenced the activity. Furthermore, the significantly bioactive compounds 6b-e, 6g, 6i and 6l showed very low toxicities against HL-7702, BEL-7402 and HCT-8 cells. Resistance development assay indicated that compounds 6b-e and 6l failed to induce the two tested strains of fungi to develop resistance. SEM analysis initially revealed that compound 6d may exert its antifungal effect by damaging fungal cell wall.
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http://dx.doi.org/10.1016/j.ejmech.2019.04.029DOI Listing
July 2019

Lithiophilic Ag Nanoparticle Layer on Cu Current Collector toward Stable Li Metal Anode.

ACS Appl Mater Interfaces 2019 Feb 14;11(8):8148-8154. Epub 2019 Feb 14.

Department of Chemistry , Southern University of Science and Technology (SUSTech) , Shenzhen , Guangdong 518055 , P. R. China.

Intractable hurdles of low Coulombic efficiency and dendritic Li formation during a repeated deposition/stripping process hinder the commercial use of  Li metal anode for next-generation battery systems. Achieving uniform Li nucleation is one of the effective strategies to address these issues, and it is of practical importance to realize this on a commercial Cu current collector that is lithiophobic. Herein, we design a nanostructured Ag lithiophilic layer on a Cu foil via an electroless plating process for a Li metal current collector. The deposition of lithiophilic Ag particles that are homogeneously distributed on the Cu foil can reduce the nucleation overpotential, realizing uniform Li nucleation and subsequently flat Li plating. As a result, a stable cycle stability of up to 360 h (1 mA cm) and an average Columbic efficiency of 94.5% for 100 cycles (1 mA cm) are achieved. Furthermore, CuAg full cells with LiFePO as a cathode exhibit good cycle performances and low polarization voltage. This approach provides another facile way for a stable lithium metal anode.
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http://dx.doi.org/10.1021/acsami.9b01521DOI Listing
February 2019
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